Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0006169

Physical Location & Seq

Genomic Coordinates Reverse (-)

7180293 .. 7182794

Gene Structure 2502 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1087200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2502 bp

ATGGATGCCAAAACCAAGCAAAGATTGGTGCTTTCAGTTCTTTTCTTCTGCTATCTCAAAACCCATGTCTCCCTTGCAGCCGACTCCATCACCGCAAACCAATCTCTCTCCGGCGACCAAACCATTGTCTCCGGGATTTTCGAGCTCGGTTTCTTCAATCCAGGTAATTCTTCAAACTTCTACATAGGCATGTGGTACACCAAACAAGTGGTATCCGAGAGAACCATAGTTTGGGTGGCAAACAGAGAAAAACCAGTTTCCGATAGATTTTCTTCGGTTTTGAGGATTTCAGATGGTAATTTAGTCCTCTTGAACGAGTCCAAAACCCCCATTTGGTCAACGAATTTAACCTCCTCCTCCGCCACCACTGCACGTCTTTTGGATACCGGAAACCTGGTCTTGAGATCGGGGTCTGGTAATATTACGTCGGAGGCGTTATGGCAAAGCTTTGATCATCCAGCTCACACATGGCTTCCAGGAGGTAGGATTGGGTTTAACACTGTTACCAAACAAACCCAAATTCTTACTTCATGGAAGAGTTCGGAGGATCCTGCACCGGGTCTTTTCTCGCTCGAGTTAGACCCCAACGGAAGCAATGCGTATCTCATACTGTGGAATAGGTCTAGACAGTATTGGACTAGTGGATCATGGGATCCAAAATCAAGGATTTTCAGCTTGGTTCCGGAAATGAGGCTCAATTATATATACAACTTCAGCTACTTTACGAACGAAACCGAAAGTTATTTCACCTATTCTGTTTATGATCCTAAGAGAATATCTCGTTTCGTGATGTATACTTCGGGGCAGATTCAACAGCTTACATGGTTGGATACCTCCAGGCAATGGAATTTGTTTTGGTCTCAACCGAGAAAGCAATGCGAAGTTTATGATTTGTGTGGCGCATTCGGAAGTTGCAATGAGGAAAGCAACCTCGCCTGTAATTGCTTGAACGGTTTCGAGCCCAAGTCGAAGAGAGATTGGGATTTGATGGACTACTCTGGTGGGTGTTCGAGAAAAACTCGTCTTTATTGTGAAAATGCTACTAGTGCTAACGGGAAGCCAGACCAGTTTCTTGAACTTCCTAGCATGTCATTGCCTGAAAATGAACAGTCTGTGGAGGTTGGGAGTTTTGCGGAATGTGAATCGTTCTGCCTTAGAAACTGCTCTTGCACTGCTTATGCTTACAACAGAAGTGGATGTTCGTTTTGGAAAGGAGAGCTTTTGGATTTGCAACAACTAAAGTCAAGTGACGACCAGGGAAGAACTTTATACCTCAAACTTGCAGCATCTGAGTTTAAGAGTTCGAAAAGTAACAAGGGATTGATTGTTGGTGTTGTGGCAGGCTCAACTGCCGGTGTAGCAGTTCTTTTAGGCCTTATTGTGTTTGCAATCTTGAGACGGAGAAAGCGAGTGAAAGGATTGGGAAAAGCAGTAGAGGGTTCGTTGGTGGCATTCGGGTACAGAGATATGCAAGATGCAACGAAGAACTTCTCTGAAAAATTGGGGGGAGGAGGTTTTGGTTCTGTTTTCAAAGGGACATTGCCCGATTCGTCTGTCATAGCAGTGAAGAAGCTTGAAAGTGTTAGCCAAGGGGAGAAGCAATTCAGAACAGAAGTGAGCACAATTGGGACAATCCAGCATGTCAATCTTGTCCGCCTTCGTGGGTTCTGCTCCGAGGGTTCTAAAAGGATGTTGGTGTATGATTACATGCCAAACGGATCTTTGGACTCTCACCTTTTCAACGATAAGGGCCCGGGGCTCTTGGATTGGAAAACAAGGTACCAAATTGCACTTGGGACAGCAAGAGGGTTGGCTTATCTTCACGAGAAGTGCAGAGATTGCATCATACATTGTGACATAAAGCCAGAAAACATCCTCTTAGACGCTGAACTTTGTCCGAAAGTGGCAGACTTTGGCCTTGCAAAGCTTGTTGGAAGAGAGTTTAGCAGGGTCCTGACAACCATGAGAGGGACAAGAGGCTATCTTGCGCCAGAGTGGATTTCAGGCGTGCCGATCACAGTGAAAGCGGATGTGTACAGCTACGGAATGATGCTGTTTGAATTCGTTTCAGGGAGAAGAAACTCGGAGCAATCTGAAGATGGGAAAGTTAGATTCTTCCCAGCTTATGCTGCTAGCCAGATTAGTACTCCAGAAAGTGATGTCTTGAGCCTATTGGATCCGAGTCTCGATGGAAATGCTGACGTAGAAGAGCTCACTAGGGTTTGTAGAGTTTCATGTTGGTGTGTCCAAGATGATGAAGCTCAGAGGCCGTCAATGGGGCAGGTGGTGCAAATCCTCGAGGCAGTTTCCGATGTCAACTTGCCACCAATTCCGAGAGCTCTTCAATTGTTTGGAGACGACCAGGAGCAGATAGTTTTCTTCACTGAGTCATCCTCGAGCCAGAGTTCGCGCCCGCGGAGCAATAACACATCGACTGCTTCCTCTCAGACCAAGAGCACCCCATCTTCAACCATATCCAAGGGATCTAAAGAGCATCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

B_lectin PAN_2 Pkinase S_locus_glycop

Protein Analysis

834

Amino Acids

92.65

Weight (kDa)

7.7

Isoelectric Point (pI)

42.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	75 - 179	7.4e-33	D-mannose binding lectin
S_locus_glycop	PF00954	212 - 323	2.7e-31	S-locus glycoprotein domain
PAN_2	PF08276	353 - 410	5.1e-15	PAN-like domain
PK_Tyr_Ser-Thr	PF07714	497 - 765	6.1e-40	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	498 - 764	1.1e-46	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006169)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19130
fragaria_vesca	FvH4_6g44310
malus_domestica	MD09G1098900.v1.1 MD17G1087200.v1.1
prunus_persica	Prupe.3G227300_v2.0.a1 Prupe.3G227500_v2.0.a1
pyrus_communis	pycom09g02260 pycom17g08440
rosa_chinensis	RchiOBHm_Chr2g0161171 RchiOBHm_Chr2g0161191
rosa_laevigata	RLG00000021264 RLG00000021266 RLG00000029382
rosa_multiflora	Rmu_sc0006420.1_g000040 Rmu_sc0006420.1_g000045
rosa_roxburghii	Rroxscaffold_2G00089040 Rroxscaffold_2G00089060
rosa_rugosa	Rorug02G0486800 Rorug02G0486900
rosa_samantha	Rh2AG553500 Rh2BG567200 Rh2CG537300 Rh2CG537500 Rh2DG576500 Rh2DG576600
rosa_wichuraiana	Rw2G045780 Rw2G045800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2272
AbsI	CCTCGAGG	1 cut(s)	2297
Acc36I	ACCTGC	1 cut(s)	2272
Acc65I	GGTACC	1 cut(s)	1779
AccB1I	GGYRCC	1 cut(s)	1779
AccB7I	CCANNNNNTGG	2 cut(s)	231, 843
AccI	GTMKAC	1 cut(s)	794
AccII	CGCG	2 cut(s)	2410, 2416
AccIII	TCCGGA	1 cut(s)	682
AciI	CCGC	7 cut(s)	93, 360, 1133, 1654, 2027, 2414, 2416
AcsI	RAATTY	4 cut(s)	343, 519, 847, 2060
AcuI	CTGAAG	2 cut(s)	697, 2115
AfaI	GTAC	5 cut(s)	197, 1460, 1781, 2036, 2146
AfiI	CCNNNNNNNGG	6 cut(s)	231, 333, 557, 843, 1968, 2276
AhlI	ACTAGT	2 cut(s)	638, 1043
AjiI	CACGTC	1 cut(s)	374
AjnI	CCWGG	6 cut(s)	160, 393, 475, 836, 1254, 2361
AjuI	GAANNNNNNNTTGG	2 cut(s)	214, 246
Alw21I	GWGCWC	5 cut(s)	147, 1622, 2214, 2341, 2459
Alw26I	GTCTC	6 cut(s)	73, 133, 864, 1390, 2189, 2349
AlwNI	CAGNNNCTG	1 cut(s)	1289
Ama87I	CYCGRG	4 cut(s)	572, 1753, 2297, 2395
Aor13HI	TCCGGA	1 cut(s)	682
AoxI	GGCC	4 cut(s)	1372, 1750, 1915, 2267
ApaI	GGGCCC	1 cut(s)	1754
ApeKI	GCWGC	3 cut(s)	77, 1283, 2129
ApoI	RAATTY	4 cut(s)	343, 519, 847, 2060
ArsI	GACNNNNNNTTYG	2 cut(s)	51, 83
Asp700I	GAANNNNTTC	3 cut(s)	953, 1487, 1601
Asp718I	GGTACC	1 cut(s)	1779
AspLEI	GCGC	3 cut(s)	902, 1990, 2412
AspS9I	GGNCC	3 cut(s)	1750, 1751, 1951
AsuC2I	CCSGG	4 cut(s)	133, 558, 1754, 1755
AsuHPI	GGTGA	3 cut(s)	82, 739, 1724
AsuII	TTCGAA	1 cut(s)	1304
AsuNHI	GCTAGC	1 cut(s)	2132
AvaI	CYCGRG	4 cut(s)	572, 1753, 2297, 2395
AvaII	GGWCC	1 cut(s)	1951
BaeGI	GKGCMC	1 cut(s)	1754
BamHI	GGATCC	3 cut(s)	547, 652, 2176
BanI	GGYRCC	1 cut(s)	1779
BanII	GRGCYC	6 cut(s)	147, 963, 1754, 1761, 2214, 2341
BauI	CACGAG	1 cut(s)	1823
Bbv12I	GWGCWC	5 cut(s)	147, 1622, 2214, 2341, 2459
BbvI	GCAGC	3 cut(s)	89, 1295, 2116
BccI	CCATC	6 cut(s)	95, 287, 982, 2093, 2183, 2470
BceAI	ACGGC	1 cut(s)	2254
BcgI	CGANNNNNNTGC	2 cut(s)	2177, 2211
BciT130I	CCWGG	6 cut(s)	162, 395, 477, 838, 1256, 2363
BciVI	GTATCC	3 cut(s)	223, 376, 823
BclI	TGATCA	1 cut(s)	451
BcnI	CCSGG	4 cut(s)	133, 558, 1754, 1755
BcoDI	GTCTC	6 cut(s)	73, 133, 864, 1390, 2189, 2349
BcuI	ACTAGT	2 cut(s)	638, 1043
BfaI	CTAG	6 cut(s)	624, 639, 1044, 1083, 2133, 2217
BfuAI	ACCTGC	1 cut(s)	2272
BfuI	GTATCC	3 cut(s)	223, 376, 823
BisI	GCNGC	3 cut(s)	78, 1284, 2130
BlsI	GCNGC	3 cut(s)	79, 1285, 2131
BmcAI	AGTACT	1 cut(s)	2146
Bme18I	GGWCC	1 cut(s)	1951
BmeT110I	CYCGRG	4 cut(s)	572, 1753, 2297, 2395
BmgBI	CACGTC	1 cut(s)	374
BmgT120I	GGNCC	3 cut(s)	1750, 1751, 1951
BmiI	GGNNCC	7 cut(s)	549, 654, 681, 1752, 1781, 1952, 2178
BmsI	GCATC	4 cut(s)	1295, 1465, 1851, 2040
BmtI	GCTAGC	1 cut(s)	2136
BplI	GAGNNNNNCTC	6 cut(s)	763, 795, 1003, 1035, 2378, 2410
BpmI	CTGGAG	2 cut(s)	820, 2133
Bpu14I	TTCGAA	1 cut(s)	1304
BpuEI	CTTGAG	3 cut(s)	421, 1414, 2185
BpuMI	CCSGG	4 cut(s)	133, 558, 1754, 1755
BsaI	GGTCTC	1 cut(s)	864
BsaJI	CCNNGG	7 cut(s)	1255, 1588, 1674, 1753, 1754, 2414, 2478
BsaWI	WCCGGW	2 cut(s)	386, 682
Bsc4I	CCNNNNNNNGG	6 cut(s)	231, 333, 557, 843, 1968, 2276
Bse118I	RCCGGY	1 cut(s)	1352
Bse1I	ACTGG	2 cut(s)	254, 1066
Bse3DI	GCAATG	6 cut(s)	601, 848, 881, 922, 1091, 1538
BseAI	TCCGGA	1 cut(s)	682
BseBI	CCWGG	6 cut(s)	162, 395, 477, 838, 1256, 2363
BseDI	CCNNGG	7 cut(s)	1255, 1588, 1674, 1753, 1754, 2414, 2478
BseGI	GGATG	7 cut(s)	10, 454, 1202, 1695, 1872, 2035, 2390
BseLI	CCNNNNNNNGG	6 cut(s)	231, 333, 557, 843, 1968, 2276
BseMI	GCAATG	6 cut(s)	601, 848, 881, 922, 1091, 1538
BseMII	CTCAG	4 cut(s)	1281, 2276, 2376, 2459
BseNI	ACTGG	2 cut(s)	254, 1066
BseRI	GAGGAG	3 cut(s)	343, 346, 1524
BseSI	GKGCMC	1 cut(s)	1754
BseXI	GCAGC	3 cut(s)	89, 1295, 2116
BseYI	CCCAGC	1 cut(s)	2119
BsgI	GTGCAG	3 cut(s)	354, 537, 1852
Bsh1236I	CGCG	2 cut(s)	2410, 2416
BshFI	GGCC	4 cut(s)	1374, 1752, 1917, 2269
BshNI	GGYRCC	1 cut(s)	1779
BsiHKAI	GWGCWC	5 cut(s)	147, 1622, 2214, 2341, 2459
BsiHKCI	CYCGRG	4 cut(s)	572, 1753, 2297, 2395
BsiSI	CCGG	7 cut(s)	111, 132, 387, 557, 683, 1353, 1754
BslFI	GGGAC	4 cut(s)	1549, 1642, 1810, 1984
BslI	CCNNNNNNNGG	6 cut(s)	231, 333, 557, 843, 1968, 2276
BsmAI	GTCTC	6 cut(s)	73, 133, 864, 1390, 2189, 2349
BsmBI	CGTCTC	2 cut(s)	1390, 2349
BsmFI	GGGAC	4 cut(s)	1549, 1642, 1810, 1984
BsmI	GAATGC	2 cut(s)	902, 1451
BsnI	GGCC	4 cut(s)	1374, 1752, 1917, 2269
Bso31I	GGTCTC	1 cut(s)	864
BsoBI	CYCGRG	4 cut(s)	572, 1753, 2297, 2395
Bsp119I	TTCGAA	1 cut(s)	1304
Bsp120I	GGGCCC	1 cut(s)	1750
Bsp1286I	GDGCHC	8 cut(s)	147, 963, 1622, 1754, 1761, 2214, 2341, 2459
Bsp13I	TCCGGA	1 cut(s)	682
Bsp1407I	TGTACA	1 cut(s)	2034
BspACI	CCGC	7 cut(s)	93, 360, 1133, 1654, 2027, 2414, 2416
BspANI	GGCC	4 cut(s)	1374, 1752, 1917, 2269
BspCNI	CTCAG	4 cut(s)	1282, 2275, 2377, 2458
BspEI	TCCGGA	1 cut(s)	682
BspFNI	CGCG	2 cut(s)	2410, 2416
BspLI	GGNNCC	7 cut(s)	549, 654, 681, 1752, 1781, 1952, 2178
BspMI	ACCTGC	1 cut(s)	2272
BspOI	GCTAGC	1 cut(s)	2136
BspQI	GCTCTTC	2 cut(s)	2202, 2346
BspT104I	TTCGAA	1 cut(s)	1304
BspT107I	GGYRCC	1 cut(s)	1779
BspTNI	GGTCTC	1 cut(s)	864
BsrDI	GCAATG	6 cut(s)	601, 848, 881, 922, 1091, 1538
BsrFI	RCCGGY	1 cut(s)	1352
BsrGI	TGTACA	1 cut(s)	2034
BsrI	ACTGG	2 cut(s)	254, 1066
BssAI	RCCGGY	1 cut(s)	1352
BssECI	CCNNGG	7 cut(s)	1255, 1588, 1674, 1753, 1754, 2414, 2478
BssNAI	GTATAC	1 cut(s)	795
BssSI	CACGAG	1 cut(s)	1823
BssT1I	CCWWGG	2 cut(s)	1588, 2478
Bst1107I	GTATAC	1 cut(s)	795
Bst2BI	CACGAG	1 cut(s)	1823
Bst2UI	CCWGG	6 cut(s)	162, 395, 477, 838, 1256, 2363
Bst4CI	ACNGT	6 cut(s)	502, 612, 630, 953, 1110, 2020
Bst6I	CTCTTC	5 cut(s)	530, 965, 1930, 2202, 2346
BstAPI	GCANNNNNTGC	2 cut(s)	1839, 2287
BstAUI	TGTACA	1 cut(s)	2034
BstBI	TTCGAA	1 cut(s)	1304
BstC8I	GCNNGC	4 cut(s)	1342, 2009, 2134, 2414
BstDEI	CTNAG	7 cut(s)	768, 1154, 1290, 1879, 2262, 2385, 2445
BstDSI	CCRYGG	1 cut(s)	2414
BstF5I	GGATG	7 cut(s)	10, 454, 1202, 1695, 1872, 2035, 2390
BstFNI	CGCG	2 cut(s)	2410, 2416
BstHHI	GCGC	3 cut(s)	902, 1990, 2412
BstMAI	GTCTC	6 cut(s)	73, 133, 864, 1390, 2189, 2349
BstMWI	GCNNNNNNNGC	7 cut(s)	368, 1046, 1358, 1839, 2129, 2287, 2418
BstNI	CCWGG	6 cut(s)	162, 395, 477, 838, 1256, 2363
BstNSI	RCATGY	4 cut(s)	193, 1090, 1643, 1711
BstSLI	GKGCMC	1 cut(s)	1754
BstUI	CGCG	2 cut(s)	2410, 2416
BstV1I	GCAGC	3 cut(s)	89, 1295, 2116
BstX2I	RGATCY	5 cut(s)	547, 652, 1718, 2176, 2483
BstXI	CCANNNNNNTGG	1 cut(s)	208
BstYI	RGATCY	5 cut(s)	547, 652, 1718, 2176, 2483
BstZ17I	GTATAC	1 cut(s)	795
BsuI	GTATCC	3 cut(s)	223, 376, 823
BsuRI	GGCC	4 cut(s)	1374, 1752, 1917, 2269
BtgI	CCRYGG	1 cut(s)	2414
BtrI	CACGTC	1 cut(s)	374
BtsCI	GGATG	7 cut(s)	10, 454, 1202, 1695, 1872, 2035, 2390
BtsI	GCAGTG	3 cut(s)	366, 1170, 1569
BtsIMutI	CAGTG	6 cut(s)	366, 498, 1170, 1569, 2025, 2382
BveI	ACCTGC	1 cut(s)	2272
Cac8I	GCNNGC	4 cut(s)	1342, 2009, 2134, 2414
CaiI	CAGNNNCTG	1 cut(s)	1289
CfoI	GCGC	3 cut(s)	902, 1990, 2412
Cfr10I	RCCGGY	1 cut(s)	1352
Cfr13I	GGNCC	3 cut(s)	1750, 1751, 1951
Cfr42I	CCGCGG	1 cut(s)	2417
Cfr9I	CCCGGG	1 cut(s)	1753
CseI	GACGC	1 cut(s)	1892
CsiI	ACCWGGT	1 cut(s)	393
Csp6I	GTAC	5 cut(s)	196, 1459, 1780, 2035, 2145
CspCI	CAANNNNNGTGG	2 cut(s)	1174, 1209
CviQI	GTAC	5 cut(s)	196, 1459, 1780, 2035, 2145
DdeI	CTNAG	7 cut(s)	768, 1154, 1290, 1879, 2262, 2385, 2445
Eam1104I	CTCTTC	5 cut(s)	530, 965, 1930, 2202, 2346
EarI	CTCTTC	5 cut(s)	530, 965, 1930, 2202, 2346
EciI	GGCGGA	2 cut(s)	349, 1643
Ecl136II	GAGCTC	3 cut(s)	145, 2212, 2339
Eco130I	CCWWGG	2 cut(s)	1588, 2478
Eco147I	AGGCCT	1 cut(s)	1374
Eco24I	GRGCYC	6 cut(s)	147, 963, 1754, 1761, 2214, 2341
Eco31I	GGTCTC	1 cut(s)	864
Eco47I	GGWCC	1 cut(s)	1951
Eco53kI	GAGCTC	3 cut(s)	145, 2212, 2339
Eco57I	CTGAAG	2 cut(s)	697, 2115
Eco88I	CYCGRG	4 cut(s)	572, 1753, 2297, 2395
EcoICRI	GAGCTC	3 cut(s)	145, 2212, 2339
EcoO109I	RGGNCCY	2 cut(s)	1750, 1951
EcoRI	GAATTC	1 cut(s)	2060
EcoRII	CCWGG	6 cut(s)	160, 393, 475, 836, 1254, 2361
EcoT14I	CCWWGG	2 cut(s)	1588, 2478
EcoT38I	GRGCYC	6 cut(s)	147, 963, 1754, 1761, 2214, 2341
ErhI	CCWWGG	2 cut(s)	1588, 2478
Esp3I	CGTCTC	2 cut(s)	1390, 2349
FalI	AAGNNNNNCTT	2 cut(s)	1203, 1235
FaqI	GGGAC	4 cut(s)	1549, 1642, 1810, 1984
FauI	CCCGC	1 cut(s)	2421
FbaI	TGATCA	1 cut(s)	451
FblI	GTMKAC	1 cut(s)	794
Fnu4HI	GCNGC	3 cut(s)	78, 1284, 2130
FokI	GGATG	7 cut(s)	17, 441, 1209, 1702, 1859, 2042, 2377
FriOI	GRGCYC	6 cut(s)	147, 963, 1754, 1761, 2214, 2341
Fsp4HI	GCNGC	3 cut(s)	78, 1284, 2130
FspBI	CTAG	6 cut(s)	624, 639, 1044, 1083, 2133, 2217
GlaI	GCGC	3 cut(s)	901, 1989, 2411
GluI	GCNGC	3 cut(s)	78, 1284, 2130
GsaI	CCCAGC	1 cut(s)	2123
GsuI	CTGGAG	2 cut(s)	820, 2133
HaeIII	GGCC	4 cut(s)	1374, 1752, 1917, 2269
HapII	CCGG	7 cut(s)	111, 132, 387, 557, 683, 1353, 1754
HgaI	GACGC	1 cut(s)	1892
HhaI	GCGC	3 cut(s)	902, 1990, 2412
Hin6I	GCGC	3 cut(s)	900, 1988, 2410
HinP1I	GCGC	3 cut(s)	900, 1988, 2410
HincII	GTYRAC	2 cut(s)	339, 2317
HindII	GTYRAC	2 cut(s)	339, 2317
HindIII	AAGCTT	3 cut(s)	445, 1571, 1925
HinfI	GANTC	9 cut(s)	83, 317, 808, 1142, 1547, 1727, 2112, 2182, 2387
HpaII	CCGG	7 cut(s)	111, 132, 387, 557, 683, 1353, 1754
HphI	GGTGA	3 cut(s)	82, 739, 1724
Hpy166II	GTNNAC	5 cut(s)	198, 339, 795, 2035, 2317
Hpy8I	GTNNAC	5 cut(s)	198, 339, 795, 2035, 2317
Hpy99I	CGWCG	1 cut(s)	430
HpyAV	CCTTC	1 cut(s)	1667
HpyCH4III	ACNGT	6 cut(s)	502, 612, 630, 953, 1110, 2020
HpyCH4IV	ACGT	3 cut(s)	373, 425, 2202
HpyF10VI	GCNNNNNNNGC	7 cut(s)	368, 1046, 1358, 1839, 2129, 2287, 2418
HpyF3I	CTNAG	7 cut(s)	768, 1154, 1290, 1879, 2262, 2385, 2445
HpySE526I	ACGT	3 cut(s)	373, 425, 2202
HspAI	GCGC	3 cut(s)	900, 1988, 2410
Kpn2I	TCCGGA	1 cut(s)	682
KpnI	GGTACC	1 cut(s)	1783
Ksp22I	TGATCA	1 cut(s)	451
KspI	CCGCGG	1 cut(s)	2417
LguI	GCTCTTC	2 cut(s)	2202, 2346
LmnI	GCTCC	4 cut(s)	1676, 2086, 2365, 2418
Lsp1109I	GCAGC	3 cut(s)	89, 1295, 2116
LweI	GCATC	4 cut(s)	1295, 1465, 1851, 2040
MabI	ACCWGGT	1 cut(s)	393
MaeI	CTAG	6 cut(s)	624, 639, 1044, 1083, 2133, 2217
MaeII	ACGT	3 cut(s)	373, 425, 2202
MaeIII	GTNAC	4 cut(s)	502, 1247, 1310, 1853
MfeI	CAATTG	2 cut(s)	1623, 2345
MflI	RGATCY	5 cut(s)	547, 652, 1718, 2176, 2483
MhlI	GDGCHC	8 cut(s)	147, 963, 1622, 1754, 1761, 2214, 2341, 2459
MlyI	GAGTC	5 cut(s)	77, 326, 1721, 2191, 2396
MmeI	TCCRAC	3 cut(s)	408, 807, 1912
MroI	TCCGGA	1 cut(s)	682
MroXI	GAANNNNTTC	3 cut(s)	953, 1487, 1601
MseI	TTAA	3 cut(s)	347, 495, 1296
MslI	CAYNNNNRTG	3 cut(s)	188, 1562, 2239
MspA1I	CMGCKG	1 cut(s)	2416
MspI	CCGG	7 cut(s)	111, 132, 387, 557, 683, 1353, 1754
MunI	CAATTG	2 cut(s)	1623, 2345
Mva1269I	GAATGC	2 cut(s)	902, 1451
MvaI	CCWGG	6 cut(s)	162, 395, 477, 838, 1256, 2363
MvnI	CGCG	2 cut(s)	2410, 2416
MwoI	GCNNNNNNNGC	7 cut(s)	368, 1046, 1358, 1839, 2129, 2287, 2418
NciI	CCSGG	4 cut(s)	133, 558, 1754, 1755
NheI	GCTAGC	1 cut(s)	2132
NlaIV	GGNNCC	7 cut(s)	549, 654, 681, 1752, 1781, 1952, 2178
NmuCI	GTSAC	2 cut(s)	1247, 1853
NspI	RCATGY	4 cut(s)	193, 1090, 1643, 1711
NspV	TTCGAA	1 cut(s)	1304
PaeR7I	CTCGAG	3 cut(s)	572, 2297, 2395
PaqCI	CACCTGC	1 cut(s)	2272
PceI	AGGCCT	1 cut(s)	1374
PciSI	GCTCTTC	2 cut(s)	2202, 2346
PcsI	WCGNNNNNNNCGW	2 cut(s)	431, 1405
PctI	GAATGC	2 cut(s)	902, 1451
PdmI	GAANNNNTTC	3 cut(s)	953, 1487, 1601
PfeI	GAWTC	4 cut(s)	808, 1142, 1547, 2112
PflMI	CCANNNNNTGG	2 cut(s)	231, 843
PfoI	TCCNGGA	2 cut(s)	131, 475
PkrI	GCNGC	3 cut(s)	79, 1285, 2131
PleI	GAGTC	5 cut(s)	77, 325, 1721, 2190, 2395
PpsI	GAGTC	5 cut(s)	77, 325, 1721, 2190, 2395
PpuMI	RGGWCCY	1 cut(s)	1951
Psp124BI	GAGCTC	3 cut(s)	147, 2214, 2341
Psp5II	RGGWCCY	1 cut(s)	1951
Psp6I	CCWGG	6 cut(s)	160, 393, 475, 836, 1254, 2361
PspFI	CCCAGC	1 cut(s)	2119
PspGI	CCWGG	6 cut(s)	160, 393, 475, 836, 1254, 2361
PspN4I	GGNNCC	7 cut(s)	549, 654, 681, 1752, 1781, 1952, 2178
PspOMI	GGGCCC	1 cut(s)	1750
PspPI	GGNCC	3 cut(s)	1750, 1751, 1951
PspPPI	RGGWCCY	1 cut(s)	1951
PspXI	VCTCGAGB	3 cut(s)	572, 2297, 2395
PstNI	CAGNNNCTG	1 cut(s)	1289
PsuI	RGATCY	5 cut(s)	547, 652, 1718, 2176, 2483
RsaI	GTAC	5 cut(s)	197, 1460, 1781, 2036, 2146
RsaNI	GTAC	5 cut(s)	196, 1459, 1780, 2035, 2145
RseI	CAYNNNNRTG	3 cut(s)	188, 1562, 2239
SacI	GAGCTC	3 cut(s)	147, 2214, 2341
SacII	CCGCGG	1 cut(s)	2417
SapI	GCTCTTC	2 cut(s)	2202, 2346
SaqAI	TTAA	3 cut(s)	347, 495, 1296
SatI	GCNGC	3 cut(s)	78, 1284, 2130
Sau96I	GGNCC	3 cut(s)	1750, 1751, 1951
ScaI	AGTACT	1 cut(s)	2146
SchI	GAGTC	5 cut(s)	77, 326, 1721, 2191, 2396
SduI	GDGCHC	8 cut(s)	147, 963, 1622, 1754, 1761, 2214, 2341, 2459
SexAI	ACCWGGT	1 cut(s)	393
SfaNI	GCATC	4 cut(s)	1295, 1465, 1851, 2040
Sfr274I	CTCGAG	3 cut(s)	572, 2297, 2395
Sfr303I	CCGCGG	1 cut(s)	2417
SfuI	TTCGAA	1 cut(s)	1304
SgrBI	CCGCGG	1 cut(s)	2417
SinI	GGWCC	1 cut(s)	1951
SlaI	CTCGAG	3 cut(s)	572, 2297, 2395
SmaI	CCCGGG	1 cut(s)	1755
SmiMI	CAYNNNNRTG	3 cut(s)	188, 1562, 2239
SmlI	CTYRAG	6 cut(s)	400, 572, 1393, 2164, 2297, 2395
SmoI	CTYRAG	6 cut(s)	400, 572, 1393, 2164, 2297, 2395
SpeI	ACTAGT	2 cut(s)	638, 1043
SseBI	AGGCCT	1 cut(s)	1374
SsiI	CCGC	7 cut(s)	93, 360, 1133, 1654, 2027, 2414, 2416
SspI	AATATT	1 cut(s)	421
SspMI	CTAG	6 cut(s)	624, 639, 1044, 1083, 2133, 2217
SstI	GAGCTC	3 cut(s)	147, 2214, 2341
StuI	AGGCCT	1 cut(s)	1374
StyI	CCWWGG	2 cut(s)	1588, 2478
TaaI	ACNGT	6 cut(s)	502, 612, 630, 953, 1110, 2020
TaiI	ACGT	3 cut(s)	376, 428, 2205
TatI	WGTACW	2 cut(s)	2034, 2144
TfiI	GAWTC	4 cut(s)	808, 1142, 1547, 2112
Tru1I	TTAA	3 cut(s)	347, 495, 1296
Tru9I	TTAA	3 cut(s)	347, 495, 1296
TscAI	CASTG	6 cut(s)	373, 505, 1177, 1569, 2025, 2389
TseFI	GTSAC	2 cut(s)	1247, 1853
TseI	GCWGC	3 cut(s)	77, 1283, 2129
Tsp45I	GTSAC	2 cut(s)	1247, 1853
TspDTI	ATGAA	4 cut(s)	519, 1119, 2223, 2271
TspGWI	ACGGA	4 cut(s)	603, 1414, 1731, 2058
TspMI	CCCGGG	1 cut(s)	1753
TspRI	CASTG	6 cut(s)	373, 505, 1177, 1569, 2025, 2389
Van91I	CCANNNNNTGG	2 cut(s)	231, 843
VpaK11BI	GGWCC	1 cut(s)	1951
XapI	RAATTY	4 cut(s)	343, 519, 847, 2060
XbaI	TCTAGA	1 cut(s)	623
XceI	RCATGY	4 cut(s)	193, 1090, 1643, 1711
XcmI	CCANNNNNNNNNTGG	4 cut(s)	22, 232, 465, 1790
XhoI	CTCGAG	3 cut(s)	572, 2297, 2395
XmaI	CCCGGG	1 cut(s)	1753
XmiI	GTMKAC	1 cut(s)	794
XmnI	GAANNNNTTC	3 cut(s)	953, 1487, 1601
XspI	CTAG	6 cut(s)	624, 639, 1044, 1083, 2133, 2217
ZrmI	AGTACT	1 cut(s)	2146

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.