MD09G1106000.v1.1

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0007613
Physical Location & Seq
Reverse (-)
7682031 .. 7684010
1980 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1106000.v1.1.491

Sequence Viewer

Length: 984 bp
ATGGCTTTTCAAAAGATCATCTCAGTCTTTCTTCTTCAGGTTCTGCTTGTTTTGTTGGCCTTAGACTCGAGCAATGCGCAGAAATTGAAAGTCGGGTTCTACTACAAAACATGCCCTGATTTAGAGGCTATTGTGGCAAGGACTACTTATCAATACATATCTAGGGCATCAACTCTTGCTGCTCCTATTCTAAGGATGCATTTTCATGATTGTTTTGTTAGGGGATGTGATGGTTCTGTGCTATTGAATTCCACACCAAACAGTCAAGCTGAGAAAGAAGCCATCCCCAACCAAAGCTTAAGAGGGTTCCATGTCATTGATGCAGTAAAATCTGCAGTAGAAAAGAAGTGTCCTGGTGTCGTTTCTTGTGCTGATATCTTGGCCTTAGTAGCTCGTGACGCAGTTAGAATGGTTCATGGCTCATTTTGGGAGGTTCCGACTGGACGAAGAGATGGAAGAGTGTCATTAGCCTCAGAGGCCAATAGAGGCTTACCTCCTCCTTTTGCTGACATAACTCAACTCAAAGCAATATTTGCAGCCAAGGGTTTAAGTGCTAAAGACCTAGCGGTTCTGTCAGGAGGACACACCATAGGCACATCTCACTGCCCGTCCTTCACAAATCGCTTGTATAACTTCACCGGAAAAGGCGACACAGACCCGAAATTGGACAAAAATTATATAGCTCGGCTGAAGACAAAATGCAAGCCAGGGGACACGACTAACCTGGTTGAGATGGATCCTGGAAGCTTCAAAACGTTTGATGAAGATTACTACACTCTAGTAACTCAAAGAAGAGGACTTTTCCAGTCTGACTCAGCTCTTCTTGATGATACTGAGACAAAAGCCTATGTGACAAAGCAAGCCACAACTCGTGGATCCACCTTTCTAACAGATTTCGGTGTCTCAATGGTGAATATGGGTAATATTGGAGTCCTCACAGGCAAAAACGGTGAAATAAGGAAGCAATGTGCTTTTGTTAATTGA

Protein Analysis

328

Amino Acids

35.69

Weight (kDa)

9.02

Isoelectric Point (pI)

25.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 50 - 289 6.6e-85 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 78
AciI CCGC 1 cut(s) 566
AclI AACGTT 1 cut(s) 755
AclWI GGATC 4 cut(s) 731, 744, 870, 883
AcsI RAATTY 1 cut(s) 247
AcuI CTGAAG 2 cut(s) 20, 710
AfiI CCNNNNNNNGG 1 cut(s) 664
AflII CTTAAG 1 cut(s) 298
AgsI TTSAA 4 cut(s) 11, 88, 247, 751
AjnI CCWGG 4 cut(s) 352, 706, 723, 739
AluBI AGCT 6 cut(s) 269, 297, 392, 683, 747, 818
AluI AGCT 6 cut(s) 269, 297, 392, 683, 747, 818
Alw26I GTCTC 2 cut(s) 830, 907
AlwI GGATC 4 cut(s) 731, 744, 870, 883
AlwNI CAGNNNCTG 1 cut(s) 43
Ama87I CYCGRG 1 cut(s) 67
AoxI GGCC 3 cut(s) 57, 381, 477
ApeKI GCWGC 2 cut(s) 179, 536
ApoI RAATTY 1 cut(s) 247
AspLEI GCGC 1 cut(s) 79
AsuHPI GGTGA 3 cut(s) 628, 922, 962
AvaI CYCGRG 1 cut(s) 67
BamHI GGATCC 2 cut(s) 736, 875
BauI CACGAG 2 cut(s) 393, 870
BbsI GAAGAC 1 cut(s) 698
BbvI GCAGC 2 cut(s) 166, 548
BccI CCATC 4 cut(s) 224, 290, 446, 727
BciT130I CCWGG 4 cut(s) 354, 708, 725, 741
BcoDI GTCTC 2 cut(s) 830, 907
BfaI CTAG 3 cut(s) 162, 563, 779
BfmI CTRYAG 1 cut(s) 333
BfrI CTTAAG 1 cut(s) 298
BglI GCCNNNNNGGC 1 cut(s) 476
BisI GCNGC 2 cut(s) 180, 537
BlsI GCNGC 2 cut(s) 181, 538
Bme1390I CCNGG 4 cut(s) 354, 708, 725, 741
BmeT110I CYCGRG 1 cut(s) 67
BmiI GGNNCC 4 cut(s) 308, 435, 738, 877
BmrFI CCNGG 4 cut(s) 354, 708, 725, 741
BmsI GCATC 3 cut(s) 176, 186, 310
BpiI GAAGAC 1 cut(s) 698
BsaJI CCNNGG 2 cut(s) 540, 707
BsaWI WCCGGW 1 cut(s) 638
Bsc4I CCNNNNNNNGG 1 cut(s) 664
Bse1I ACTGG 2 cut(s) 445, 805
Bse3DI GCAATG 2 cut(s) 79, 971
BseBI CCWGG 4 cut(s) 354, 708, 725, 741
BseDI CCNNGG 2 cut(s) 540, 707
BseGI GGATG 3 cut(s) 201, 230, 282
BseLI CCNNNNNNNGG 1 cut(s) 664
BseMI GCAATG 2 cut(s) 79, 971
BseMII CTCAG 5 cut(s) 36, 261, 486, 825, 828
BseNI ACTGG 2 cut(s) 445, 805
BseRI GAGGAG 1 cut(s) 486
BseXI GCAGC 2 cut(s) 166, 548
BshFI GGCC 3 cut(s) 59, 383, 479
BsiHKCI CYCGRG 1 cut(s) 67
BsiSI CCGG 1 cut(s) 639
BslFI GGGAC 1 cut(s) 725
BslI CCNNNNNNNGG 1 cut(s) 664
BsmAI GTCTC 2 cut(s) 830, 907
BsmFI GGGAC 1 cut(s) 725
BsnI GGCC 3 cut(s) 59, 383, 479
BsoBI CYCGRG 1 cut(s) 67
Bsp143I GATC 3 cut(s) 15, 736, 875
BspACI CCGC 1 cut(s) 566
BspANI GGCC 3 cut(s) 59, 383, 479
BspCNI CTCAG 5 cut(s) 35, 262, 485, 826, 827
BspHI TCATGA 1 cut(s) 205
BspLI GGNNCC 4 cut(s) 308, 435, 738, 877
BspMAI CTGCAG 1 cut(s) 337
BspPI GGATC 4 cut(s) 731, 744, 870, 883
BspQI GCTCTTC 1 cut(s) 825
BspTI CTTAAG 1 cut(s) 298
BsrDI GCAATG 2 cut(s) 79, 971
BsrI ACTGG 2 cut(s) 445, 805
BssECI CCNNGG 2 cut(s) 540, 707
BssMI GATC 3 cut(s) 15, 736, 875
BssSI CACGAG 2 cut(s) 393, 870
BssT1I CCWWGG 1 cut(s) 540
Bst2BI CACGAG 2 cut(s) 393, 870
Bst2UI CCWGG 4 cut(s) 354, 708, 725, 741
Bst4CI ACNGT 2 cut(s) 263, 950
Bst6I CTCTTC 4 cut(s) 442, 451, 787, 825
BstAFI CTTAAG 1 cut(s) 298
BstAPI GCANNNNNTGC 1 cut(s) 533
BstC8I GCNNGC 2 cut(s) 704, 861
BstDEI CTNAG 8 cut(s) 22, 61, 191, 270, 385, 472, 814, 834
BstF5I GGATG 3 cut(s) 201, 230, 282
BstHHI GCGC 1 cut(s) 79
BstKTI GATC 3 cut(s) 18, 739, 878
BstMAI GTCTC 2 cut(s) 830, 907
BstMBI GATC 3 cut(s) 15, 736, 875
BstMWI GCNNNNNNNGC 5 cut(s) 134, 389, 398, 476, 533
BstNI CCWGG 4 cut(s) 354, 708, 725, 741
BstNSI RCATGY 1 cut(s) 114
BstSCI CCNGG 4 cut(s) 352, 706, 723, 739
BstSFI CTRYAG 1 cut(s) 333
BstV1I GCAGC 2 cut(s) 166, 548
BstV2I GAAGAC 1 cut(s) 698
BstX2I RGATCY 2 cut(s) 736, 875
BstYI RGATCY 2 cut(s) 736, 875
BsuRI GGCC 3 cut(s) 59, 383, 479
BtsCI GGATG 3 cut(s) 201, 230, 282
BtsI GCAGTG 1 cut(s) 601
BtsIMutI CAGTG 1 cut(s) 601
Cac8I GCNNGC 2 cut(s) 704, 861
CaiI CAGNNNCTG 1 cut(s) 43
CciI TCATGA 1 cut(s) 205
CfoI GCGC 1 cut(s) 79
CseI GACGC 1 cut(s) 407
CsiI ACCWGGT 1 cut(s) 723
CviAII CATG 4 cut(s) 111, 206, 311, 416
DdeI CTNAG 8 cut(s) 22, 61, 191, 270, 385, 472, 814, 834
DpnI GATC 3 cut(s) 17, 738, 877
DpnII GATC 3 cut(s) 15, 736, 875
Eam1104I CTCTTC 4 cut(s) 442, 451, 787, 825
EarI CTCTTC 4 cut(s) 442, 451, 787, 825
Eco130I CCWWGG 1 cut(s) 540
Eco32I GATATC 1 cut(s) 376
Eco57I CTGAAG 2 cut(s) 20, 710
Eco88I CYCGRG 1 cut(s) 67
EcoRI GAATTC 1 cut(s) 247
EcoRII CCWGG 4 cut(s) 352, 706, 723, 739
EcoRV GATATC 1 cut(s) 376
EcoT14I CCWWGG 1 cut(s) 540
EcoT22I ATGCAT 1 cut(s) 201
ErhI CCWWGG 1 cut(s) 540
FaeI CATG 4 cut(s) 114, 209, 314, 419
FalI AAGNNNNNCTT 2 cut(s) 130, 162
FaqI GGGAC 1 cut(s) 725
FatI CATG 4 cut(s) 110, 205, 310, 415
Fnu4HI GCNGC 2 cut(s) 180, 537
FokI GGATG 3 cut(s) 208, 237, 269
Fsp4HI GCNGC 2 cut(s) 180, 537
FspBI CTAG 3 cut(s) 162, 563, 779
FspI TGCGCA 1 cut(s) 78
GlaI GCGC 1 cut(s) 78
GluI GCNGC 2 cut(s) 180, 537
HaeIII GGCC 3 cut(s) 59, 383, 479
HapII CCGG 1 cut(s) 639
HgaI GACGC 1 cut(s) 407
HhaI GCGC 1 cut(s) 79
Hin1II CATG 4 cut(s) 114, 209, 314, 419
Hin6I GCGC 1 cut(s) 77
HinP1I GCGC 1 cut(s) 77
HindIII AAGCTT 2 cut(s) 295, 745
HinfI GANTC 3 cut(s) 65, 812, 930
HpaII CCGG 1 cut(s) 639
HphI GGTGA 3 cut(s) 628, 922, 962
Hpy188I TCNGA 3 cut(s) 438, 475, 811
Hpy188III TCNNGA 4 cut(s) 206, 395, 576, 824
HpyAV CCTTC 1 cut(s) 622
HpyCH4III ACNGT 2 cut(s) 263, 950
HpyCH4IV ACGT 1 cut(s) 755
HpyCH4V TGCA 5 cut(s) 199, 323, 335, 536, 702
HpyF10VI GCNNNNNNNGC 5 cut(s) 134, 389, 398, 476, 533
HpyF3I CTNAG 8 cut(s) 22, 61, 191, 270, 385, 472, 814, 834
HpySE526I ACGT 1 cut(s) 755
Hsp92II CATG 4 cut(s) 114, 209, 314, 419
HspAI GCGC 1 cut(s) 77
Kzo9I GATC 3 cut(s) 15, 736, 875
LguI GCTCTTC 1 cut(s) 825
LmnI GCTCC 1 cut(s) 187
Lsp1109I GCAGC 2 cut(s) 166, 548
LweI GCATC 3 cut(s) 176, 186, 310
MabI ACCWGGT 1 cut(s) 723
MaeI CTAG 3 cut(s) 162, 563, 779
MaeII ACGT 1 cut(s) 755
MaeIII GTNAC 3 cut(s) 395, 781, 850
MalI GATC 3 cut(s) 17, 738, 877
MboI GATC 3 cut(s) 15, 736, 875
MboII GAAGA 8 cut(s) 23, 26, 459, 468, 703, 776, 804, 812
MflI RGATCY 2 cut(s) 736, 875
MluCI AATT 5 cut(s) 83, 247, 662, 673, 979
MlyI GAGTC 3 cut(s) 59, 806, 939
MmeI TCCRAC 1 cut(s) 461
Mph1103I ATGCAT 1 cut(s) 201
MseI TTAA 3 cut(s) 299, 548, 978
MslI CAYNNNNRTG 1 cut(s) 204
MspCI CTTAAG 1 cut(s) 298
MspI CCGG 1 cut(s) 639
MspR9I CCNGG 4 cut(s) 354, 708, 725, 741
MvaI CCWGG 4 cut(s) 354, 708, 725, 741
MwoI GCNNNNNNNGC 5 cut(s) 134, 389, 398, 476, 533
NdeII GATC 3 cut(s) 15, 736, 875
NlaIII CATG 4 cut(s) 114, 209, 314, 419
NlaIV GGNNCC 4 cut(s) 308, 435, 738, 877
NmeAIII GCCGAG 1 cut(s) 664
NmuCI GTSAC 2 cut(s) 395, 850
NsbI TGCGCA 1 cut(s) 78
NsiI ATGCAT 1 cut(s) 201
NspI RCATGY 1 cut(s) 114
PaeR7I CTCGAG 1 cut(s) 67
PagI TCATGA 1 cut(s) 205
PciSI GCTCTTC 1 cut(s) 825
PfoI TCCNGGA 1 cut(s) 739
PkrI GCNGC 2 cut(s) 181, 538
PleI GAGTC 3 cut(s) 59, 806, 938
PpsI GAGTC 3 cut(s) 59, 806, 938
Psp1406I AACGTT 1 cut(s) 755
Psp6I CCWGG 4 cut(s) 352, 706, 723, 739
PspGI CCWGG 4 cut(s) 352, 706, 723, 739
PspN4I GGNNCC 4 cut(s) 308, 435, 738, 877
PspXI VCTCGAGB 1 cut(s) 67
PstI CTGCAG 1 cut(s) 337
PstNI CAGNNNCTG 1 cut(s) 43
PsuI RGATCY 2 cut(s) 736, 875
RseI CAYNNNNRTG 1 cut(s) 204
SapI GCTCTTC 1 cut(s) 825
SaqAI TTAA 3 cut(s) 299, 548, 978
SatI GCNGC 2 cut(s) 180, 537
Sau3AI GATC 3 cut(s) 15, 736, 875
SchI GAGTC 3 cut(s) 59, 806, 939
ScrFI CCNGG 4 cut(s) 354, 708, 725, 741
SexAI ACCWGGT 1 cut(s) 723
SfaNI GCATC 3 cut(s) 176, 186, 310
SfcI CTRYAG 1 cut(s) 333
Sfr274I CTCGAG 1 cut(s) 67
SlaI CTCGAG 1 cut(s) 67
SmiMI CAYNNNNRTG 1 cut(s) 204
SmlI CTYRAG 2 cut(s) 67, 298
SmoI CTYRAG 2 cut(s) 67, 298
Sse9I AATT 5 cut(s) 83, 247, 662, 673, 979
SsiI CCGC 1 cut(s) 566
SspI AATATT 2 cut(s) 531, 925
SspMI CTAG 3 cut(s) 162, 563, 779
StyD4I CCNGG 4 cut(s) 352, 706, 723, 739
StyI CCWWGG 1 cut(s) 540
TaaI ACNGT 2 cut(s) 263, 950
TaiI ACGT 1 cut(s) 758
TaqI TCGA 1 cut(s) 68
TasI AATT 5 cut(s) 83, 247, 662, 673, 979
Tru1I TTAA 3 cut(s) 299, 548, 978
Tru9I TTAA 3 cut(s) 299, 548, 978
TscAI CASTG 1 cut(s) 608
TseFI GTSAC 2 cut(s) 395, 850
TseI GCWGC 2 cut(s) 179, 536
Tsp45I GTSAC 2 cut(s) 395, 850
TspDTI ATGAA 3 cut(s) 194, 404, 777
TspRI CASTG 1 cut(s) 608
Vha464I CTTAAG 1 cut(s) 298
XapI RAATTY 1 cut(s) 247
XceI RCATGY 1 cut(s) 114
XhoI CTCGAG 1 cut(s) 67
XspI CTAG 3 cut(s) 162, 563, 779
Zsp2I ATGCAT 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.