MD09G1131700.v1.1

Rrp15p

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0009316
Physical Location & Seq
Forward (+)
10137417 .. 10140187
2771 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1131700.v1.1.491

Sequence Viewer

Length: 975 bp
ATGGCCGAAGAAAACCATCTTCCAGAAGCAGCAGCAGAGCCGAGGAAGCGGAAAATAGGGAAGAAGAAGGGCTCCTGGGCAAAGAAGAGGGCGAAGATGTTACATTCGTCGGAGGAAATCAGAGCCATGAAGCCGAAGAAGATTAACAAGAAAATGAAGAAACTGTTTCTGAAACGAGCCCGGGAGTACGATTCGGAGGACGAGGAGGAGTCCAAACAGGAACATGTGAAACCGAAACCGAAACCGAAACCGCAACCGCAACCGTTGGGAAACAATGAGGAGGAGCCGAGTGGCAGTGGCGGGTCGTCTTCAGGGGAAGAGGCGGAGGAAGGTGGTGGTGGTCACAATAGGGATGATGGTTTTTCGGATGACGAGGAGCACGGCGAGTTTCTGCCCGGAATCATGAAGTTTTCGGAGGGTTGCAAGGCGTTTAGGTTGGCATTCAAGAGTATAATCAAGAAGTCTGTTTCTCAGGATGCAGTGGGACCCGTGATGTCGGCGCACAAGAAGCTTGTTGCAGAAAAGCTTGCCGAGGAGCAGACCGAGCGCAAGGCAAAGGGCGAGGCTAAGAAGGAAAAGCACACGGTGATAGAAAAGGGGCATGTGATGCCGGCCAACTATTTAGACACGCACGAAAAGTTTCTAATCGGTGTTGCTACTAAAGGAGTGGTGAAGTTGTTCAATGCTGTCAACAAGGCACAAAATGCTCAGAAAGGACTGAATCCTTCTAAGTTTAAAGATGCCAAAGTGATAAAAAAGCGGAGGAAAGAAGCATTCTTTTCCGAGTTGGGCAAAACATCTTTGAAGGCTCATACGTCTAAGGGCGCAGTGGATGGTGAAGGGCCTGCCTGGGCACCATTAAGAGATAATTACATGCTCACAAATCCCAAATTGAAAGACTGGGATAAGATGCCGGATACCGTTGCGGAAGATGACATTGGAAGGGTGGCTGGAGATAGTTCCGATGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rrp15p

Protein Analysis

325

Amino Acids

35.92

Weight (kDa)

9.25

Isoelectric Point (pI)

47.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rrp15p PF07890 144 - 263 3.9e-21 Rrp15p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 853
AciI CCGC 7 cut(s) 49, 251, 257, 300, 323, 760, 926
AcoI YGGCCR 2 cut(s) 3, 612
AcuI CTGAAG 1 cut(s) 294
AdeI CACNNNGTG 1 cut(s) 586
AfaI GTAC 1 cut(s) 188
AflIII ACRYGT 1 cut(s) 223
AgsI TTSAA 4 cut(s) 445, 682, 805, 895
AjnI CCWGG 2 cut(s) 74, 848
AjuI GAANNNNNNNTTGG 2 cut(s) 921, 953
AluBI AGCT 2 cut(s) 511, 526
AluI AGCT 2 cut(s) 511, 526
Alw21I GWGCWC 1 cut(s) 381
Ama87I CYCGRG 1 cut(s) 180
AoxI GGCC 3 cut(s) 3, 612, 842
ApeKI GCWGC 2 cut(s) 29, 32
Asp700I GAANNNNTTC 2 cut(s) 639, 677
AspLEI GCGC 3 cut(s) 502, 549, 827
AspS9I GGNCC 2 cut(s) 485, 842
AsuC2I CCSGG 3 cut(s) 181, 182, 396
AsuHPI GGTGA 3 cut(s) 598, 682, 848
AvaI CYCGRG 1 cut(s) 180
AvaII GGWCC 1 cut(s) 485
BaeGI GKGCMC 1 cut(s) 856
BanI GGYRCC 1 cut(s) 853
BanII GRGCYC 2 cut(s) 74, 181
BbsI GAAGAC 1 cut(s) 300
Bbv12I GWGCWC 1 cut(s) 381
BbvI GCAGC 2 cut(s) 41, 44
BccI CCATC 3 cut(s) 24, 350, 827
BceAI ACGGC 1 cut(s) 397
BciT130I CCWGG 2 cut(s) 76, 850
BciVI GTATCC 1 cut(s) 910
BcnI CCSGG 3 cut(s) 181, 182, 396
BfuI GTATCC 1 cut(s) 910
BisI GCNGC 2 cut(s) 30, 33
BlsI GCNGC 2 cut(s) 31, 34
Bme1390I CCNGG 5 cut(s) 76, 181, 182, 396, 850
Bme18I GGWCC 1 cut(s) 485
BmeT110I CYCGRG 1 cut(s) 180
BmgT120I GGNCC 2 cut(s) 485, 842
BmiI GGNNCC 5 cut(s) 73, 285, 486, 487, 855
BmrFI CCNGG 5 cut(s) 76, 181, 182, 396, 850
BmrI ACTGGG 1 cut(s) 910
BmsI GCATC 4 cut(s) 466, 597, 730, 900
BmuI ACTGGG 1 cut(s) 910
BpiI GAAGAC 1 cut(s) 300
BpmI CTGGAG 1 cut(s) 972
BpuMI CCSGG 3 cut(s) 181, 182, 396
BsaJI CCNNGG 5 cut(s) 41, 75, 180, 531, 849
Bse118I RCCGGY 1 cut(s) 610
Bse1I ACTGG 1 cut(s) 905
BseBI CCWGG 2 cut(s) 76, 850
BseDI CCNNGG 5 cut(s) 41, 75, 180, 531, 849
BseGI GGATG 4 cut(s) 358, 373, 481, 838
BseMII CTCAG 2 cut(s) 485, 722
BseNI ACTGG 1 cut(s) 905
BseRI GAGGAG 6 cut(s) 218, 221, 293, 296, 389, 548
BseSI GKGCMC 1 cut(s) 856
BseXI GCAGC 2 cut(s) 41, 44
BshFI GGCC 3 cut(s) 5, 614, 844
BshNI GGYRCC 1 cut(s) 853
BsiHKAI GWGCWC 1 cut(s) 381
BsiHKCI CYCGRG 1 cut(s) 180
BsiSI CCGG 4 cut(s) 181, 396, 611, 914
BslFI GGGAC 1 cut(s) 498
BsmFI GGGAC 1 cut(s) 498
BsmI GAATGC 2 cut(s) 440, 773
BsnI GGCC 3 cut(s) 5, 614, 844
BsoBI CYCGRG 1 cut(s) 180
Bsp1286I GDGCHC 4 cut(s) 74, 181, 381, 856
BspACI CCGC 7 cut(s) 49, 251, 257, 300, 323, 760, 926
BspANI GGCC 3 cut(s) 5, 614, 844
BspCNI CTCAG 2 cut(s) 484, 721
BspHI TCATGA 1 cut(s) 402
BspLI GGNNCC 5 cut(s) 73, 285, 486, 487, 855
BspT107I GGYRCC 1 cut(s) 853
BsrFI RCCGGY 1 cut(s) 610
BsrI ACTGG 1 cut(s) 905
BssAI RCCGGY 1 cut(s) 610
BssECI CCNNGG 5 cut(s) 41, 75, 180, 531, 849
Bst2UI CCWGG 2 cut(s) 76, 850
Bst4CI ACNGT 4 cut(s) 165, 264, 586, 922
Bst6I CTCTTC 2 cut(s) 80, 312
BstAPI GCANNNNNTGC 2 cut(s) 607, 704
BstC8I GCNNGC 3 cut(s) 528, 612, 846
BstDEI CTNAG 5 cut(s) 471, 567, 708, 729, 819
BstF5I GGATG 4 cut(s) 358, 373, 481, 838
BstHHI GCGC 3 cut(s) 502, 549, 827
BstMWI GCNNNNNNNGC 5 cut(s) 46, 508, 544, 607, 704
BstNI CCWGG 2 cut(s) 76, 850
BstNSI RCATGY 3 cut(s) 227, 605, 877
BstSCI CCNGG 5 cut(s) 74, 179, 180, 394, 848
BstSLI GKGCMC 1 cut(s) 856
BstV1I GCAGC 2 cut(s) 41, 44
BstV2I GAAGAC 1 cut(s) 300
BsuI GTATCC 1 cut(s) 910
BsuRI GGCC 3 cut(s) 5, 614, 844
BtsCI GGATG 4 cut(s) 358, 373, 481, 838
BtsI GCAGTG 3 cut(s) 301, 486, 834
BtsIMutI CAGTG 3 cut(s) 301, 486, 834
Cac8I GCNNGC 3 cut(s) 528, 612, 846
CciI TCATGA 1 cut(s) 402
CfoI GCGC 3 cut(s) 502, 549, 827
Cfr10I RCCGGY 1 cut(s) 610
Cfr13I GGNCC 2 cut(s) 485, 842
Cfr9I CCCGGG 1 cut(s) 180
Csp6I GTAC 1 cut(s) 187
CviAII CATG 5 cut(s) 127, 224, 403, 602, 874
CviQI GTAC 1 cut(s) 187
DdeI CTNAG 5 cut(s) 471, 567, 708, 729, 819
DraI TTTAAA 1 cut(s) 736
DraIII CACNNNGTG 1 cut(s) 586
EaeI YGGCCR 2 cut(s) 3, 612
Eam1104I CTCTTC 2 cut(s) 80, 312
EarI CTCTTC 2 cut(s) 80, 312
EciI GGCGGA 1 cut(s) 338
Eco24I GRGCYC 2 cut(s) 74, 181
Eco47I GGWCC 1 cut(s) 485
Eco57I CTGAAG 1 cut(s) 294
Eco88I CYCGRG 1 cut(s) 180
EcoO109I RGGNCCY 2 cut(s) 485, 842
EcoRII CCWGG 2 cut(s) 74, 848
EcoT38I GRGCYC 2 cut(s) 74, 181
FaeI CATG 5 cut(s) 130, 227, 406, 605, 877
FaiI YATR 7 cut(s) 128, 225, 404, 452, 603, 813, 875
FaqI GGGAC 1 cut(s) 498
FatI CATG 5 cut(s) 126, 223, 402, 601, 873
FauI CCCGC 1 cut(s) 293
Fnu4HI GCNGC 2 cut(s) 30, 33
FokI GGATG 4 cut(s) 365, 380, 488, 845
FriOI GRGCYC 2 cut(s) 74, 181
Fsp4HI GCNGC 2 cut(s) 30, 33
GlaI GCGC 3 cut(s) 501, 548, 826
GluI GCNGC 2 cut(s) 30, 33
GsuI CTGGAG 1 cut(s) 972
HaeIII GGCC 3 cut(s) 5, 614, 844
HapII CCGG 4 cut(s) 181, 396, 611, 914
HhaI GCGC 3 cut(s) 502, 549, 827
Hin1II CATG 5 cut(s) 130, 227, 406, 605, 877
Hin6I GCGC 3 cut(s) 500, 547, 825
HinP1I GCGC 3 cut(s) 500, 547, 825
HincII GTYRAC 1 cut(s) 691
HindII GTYRAC 1 cut(s) 691
HindIII AAGCTT 2 cut(s) 509, 524
HinfI GANTC 4 cut(s) 191, 209, 399, 721
HpaII CCGG 4 cut(s) 181, 396, 611, 914
HphI GGTGA 3 cut(s) 598, 682, 848
Hpy166II GTNNAC 1 cut(s) 691
Hpy188I TCNGA 9 cut(s) 112, 122, 171, 196, 367, 415, 711, 784, 964
Hpy188III TCNNGA 5 cut(s) 23, 403, 445, 457, 473
Hpy8I GTNNAC 1 cut(s) 691
Hpy99I CGWCG 1 cut(s) 112
HpyAV CCTTC 7 cut(s) 61, 323, 565, 735, 799, 833, 936
HpyCH4III ACNGT 4 cut(s) 165, 264, 586, 922
HpyCH4IV ACGT 1 cut(s) 815
HpyCH4V TGCA 3 cut(s) 423, 479, 518
HpyF10VI GCNNNNNNNGC 5 cut(s) 46, 508, 544, 607, 704
HpyF3I CTNAG 5 cut(s) 471, 567, 708, 729, 819
HpySE526I ACGT 1 cut(s) 815
Hsp92II CATG 5 cut(s) 130, 227, 406, 605, 877
HspAI GCGC 3 cut(s) 500, 547, 825
KflI GGGWCCC 1 cut(s) 485
KroI GCCGGC 1 cut(s) 610
KroNI GCCGGC 1 cut(s) 612
LmnI GCTCC 4 cut(s) 77, 283, 376, 535
Lsp1109I GCAGC 2 cut(s) 41, 44
LweI GCATC 4 cut(s) 466, 597, 730, 900
MaeII ACGT 1 cut(s) 815
MaeIII GTNAC 2 cut(s) 99, 341
MhlI GDGCHC 4 cut(s) 74, 181, 381, 856
MluCI AATT 2 cut(s) 868, 890
MlyI GAGTC 1 cut(s) 218
MmeI TCCRAC 1 cut(s) 90
MroNI GCCGGC 1 cut(s) 610
MroXI GAANNNNTTC 2 cut(s) 639, 677
MseI TTAA 3 cut(s) 144, 735, 860
MspI CCGG 4 cut(s) 181, 396, 611, 914
MspR9I CCNGG 5 cut(s) 76, 181, 182, 396, 850
Mva1269I GAATGC 2 cut(s) 440, 773
MvaI CCWGG 2 cut(s) 76, 850
MwoI GCNNNNNNNGC 5 cut(s) 46, 508, 544, 607, 704
NaeI GCCGGC 1 cut(s) 612
NciI CCSGG 3 cut(s) 181, 182, 396
NgoMIV GCCGGC 1 cut(s) 610
NlaIII CATG 5 cut(s) 130, 227, 406, 605, 877
NlaIV GGNNCC 5 cut(s) 73, 285, 486, 487, 855
NmeAIII GCCGAG 3 cut(s) 66, 312, 556
NmuCI GTSAC 1 cut(s) 341
NspI RCATGY 3 cut(s) 227, 605, 877
PagI TCATGA 1 cut(s) 402
PciI ACATGT 1 cut(s) 223
PctI GAATGC 2 cut(s) 440, 773
PdiI GCCGGC 1 cut(s) 612
PdmI GAANNNNTTC 2 cut(s) 639, 677
PfeI GAWTC 3 cut(s) 191, 399, 721
PkrI GCNGC 2 cut(s) 31, 34
PleI GAGTC 1 cut(s) 217
PpsI GAGTC 1 cut(s) 217
PpuMI RGGWCCY 1 cut(s) 485
PscI ACATGT 1 cut(s) 223
Psp5II RGGWCCY 1 cut(s) 485
Psp6I CCWGG 2 cut(s) 74, 848
PspGI CCWGG 2 cut(s) 74, 848
PspN4I GGNNCC 5 cut(s) 73, 285, 486, 487, 855
PspPI GGNCC 2 cut(s) 485, 842
PspPPI RGGWCCY 1 cut(s) 485
RsaI GTAC 1 cut(s) 188
RsaNI GTAC 1 cut(s) 187
SaqAI TTAA 3 cut(s) 144, 735, 860
SatI GCNGC 2 cut(s) 30, 33
Sau96I GGNCC 2 cut(s) 485, 842
SchI GAGTC 1 cut(s) 218
ScrFI CCNGG 5 cut(s) 76, 181, 182, 396, 850
SduI GDGCHC 4 cut(s) 74, 181, 381, 856
SetI ASST 5 cut(s) 334, 437, 513, 528, 818
SfaNI GCATC 4 cut(s) 466, 597, 730, 900
SinI GGWCC 1 cut(s) 485
SmaI CCCGGG 1 cut(s) 182
Sse9I AATT 2 cut(s) 868, 890
SsiI CCGC 7 cut(s) 49, 251, 257, 300, 323, 760, 926
StyD4I CCNGG 5 cut(s) 74, 179, 180, 394, 848
TaaI ACNGT 4 cut(s) 165, 264, 586, 922
TaiI ACGT 1 cut(s) 818
TaqII GACCGA 1 cut(s) 557
TasI AATT 2 cut(s) 868, 890
TfiI GAWTC 3 cut(s) 191, 399, 721
Tru1I TTAA 3 cut(s) 144, 735, 860
Tru9I TTAA 3 cut(s) 144, 735, 860
TscAI CASTG 3 cut(s) 301, 486, 834
TseFI GTSAC 1 cut(s) 341
TseI GCWGC 2 cut(s) 29, 32
Tsp45I GTSAC 1 cut(s) 341
TspDTI ATGAA 3 cut(s) 143, 170, 419
TspMI CCCGGG 1 cut(s) 180
TspRI CASTG 3 cut(s) 301, 486, 834
VpaK11BI GGWCC 1 cut(s) 485
XceI RCATGY 3 cut(s) 227, 605, 877
XmaI CCCGGG 1 cut(s) 180
XmnI GAANNNNTTC 2 cut(s) 639, 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.