Rw2G042280

Rrp15p

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0009316
Physical Location & Seq
Reverse (-)
68581156 .. 68584167
3012 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G042280.1

Sequence Viewer

Length: 957 bp
ATGGCCGGAGAAACCCAATTGGCAGAAGCAGCAAAAGGTCCAAACAAGAGGCGAATAGGGAAGACGAAGGGCTCTAAAGCCAGAAAGAAAGCAAAGAAGATGCCGCACGCGTCAGACGGCAGCAGAGCCATGAAGCCCAAGAAGATTGACCCGAAAATGAAGAAGCTTTATCGAAAACGGGCCAGAGAGTACGATTCAGAGGAGGAGAGTGGTGGGTCTGAAGAGGAAGGAGAAGAAAAAGAGGCGGAAGCTAAGGAGAGGCGTAAAGCAGAGGAAGGGTTCTCGGATGATGAGGAAGACGATGAAGTTCAGCCCGGAATTACGAAACTTTCAGAGGGGTGCAATGCGTTTAGGTTGGCTTTCAGGAGCATAATCAAGAAGAGTGTTGCTGACGATGTAATGGGACCAGTGTTATCAGGGAACAAGAAGCTTATTGCAGAAAAGCTGGCTGAAGAGGATGCTGAGCGCAAGGTCAAGGGAGAGGCTAAGGAAAAACAACTGGTAATAGAAAAGGGGCATGTTGTTAAACCTGTCAACTATGACCCGCATGAAAAGTTTCTAATTAGTCTTGCTACTAGAGGAGTGGTCACATTGTTCAATGCTGTCAGCAAGGCACAGAATGCTCAGAAAGGACTGGATCCTTCCAGGTTTAAAGATGCAAAAGTGATAAGAAAGCGGAGGAAAGAAGCCTTCTTTTCAGAGTTGGGCAAAACTAGTGCTTCTGCAAAGGTTCAGATGTCTAAAGGCTCAGTGGAGAATGAAGGGCCTGCCTGGGCTCCATTGCGAGATAATTTCATGCTAACAAATTCCAAGTTGAAGGACTGGGATAAAATGACGCCACTTTATAATAGTGTCATCAGAGTAGGAGGACAAAAAAATATAACAACTGGTATTATCAACATGAAGCATAAAATATGGCTGGAAGACCAAAATGGATCCTCCCTGGAGAATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rrp15p

Protein Analysis

318

Amino Acids

35.49

Weight (kDa)

9.5

Isoelectric Point (pI)

41.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rrp15p PF07890 117 - 235 1.1e-17 Rrp15p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 846
AccII CGCG 1 cut(s) 110
AciI CCGC 4 cut(s) 104, 245, 545, 676
AclWI GGATC 4 cut(s) 632, 645, 930, 943
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 805
AcuI CTGAAG 2 cut(s) 240, 471
AcyI GRCGYC 1 cut(s) 836
AfaI GTAC 1 cut(s) 191
AflIII ACRYGT 1 cut(s) 108
AgsI TTSAA 2 cut(s) 598, 817
AhlI ACTAGT 1 cut(s) 713
AjnI CCWGG 3 cut(s) 644, 770, 942
AluBI AGCT 4 cut(s) 166, 251, 430, 445
AluI AGCT 4 cut(s) 166, 251, 430, 445
AlwI GGATC 4 cut(s) 632, 645, 930, 943
AoxI GGCC 3 cut(s) 3, 180, 764
ApeKI GCWGC 2 cut(s) 29, 120
ApoI RAATTY 1 cut(s) 805
Asp700I GAANNNNTTC 1 cut(s) 555
AspLEI GCGC 1 cut(s) 468
AspS9I GGNCC 4 cut(s) 38, 180, 404, 764
AsuC2I CCSGG 1 cut(s) 315
AvaII GGWCC 2 cut(s) 38, 404
BamHI GGATCC 2 cut(s) 637, 935
BanII GRGCYC 2 cut(s) 74, 778
BbsI GAAGAC 3 cut(s) 68, 303, 930
BbvI GCAGC 2 cut(s) 41, 132
BceAI ACGGC 1 cut(s) 133
BciT130I CCWGG 3 cut(s) 646, 772, 944
BcnI CCSGG 1 cut(s) 315
BcuI ACTAGT 1 cut(s) 713
BfaI CTAG 2 cut(s) 576, 714
BisI GCNGC 3 cut(s) 30, 104, 121
BlpI GCTNAGC 1 cut(s) 462
BlsI GCNGC 3 cut(s) 31, 105, 122
Bme1390I CCNGG 4 cut(s) 315, 646, 772, 944
Bme18I GGWCC 2 cut(s) 38, 404
BmgT120I GGNCC 4 cut(s) 38, 180, 404, 764
BmiI GGNNCC 4 cut(s) 405, 639, 777, 937
BmrFI CCNGG 4 cut(s) 315, 646, 772, 944
BmrI ACTGGG 1 cut(s) 832
BmsI GCATC 3 cut(s) 90, 448, 646
BmuI ACTGGG 1 cut(s) 832
BpiI GAAGAC 3 cut(s) 68, 303, 930
Bpu10I CCTNAGC 2 cut(s) 252, 486
Bpu1102I GCTNAGC 1 cut(s) 462
BpuMI CCSGG 1 cut(s) 315
BsaHI GRCGYC 1 cut(s) 836
BsaJI CCNNGG 2 cut(s) 771, 942
Bse1I ACTGG 5 cut(s) 407, 504, 639, 827, 892
Bse3DI GCAATG 2 cut(s) 349, 779
BseBI CCWGG 3 cut(s) 646, 772, 944
BseDI CCNNGG 2 cut(s) 771, 942
BseGI GGATG 2 cut(s) 292, 463
BseMI GCAATG 2 cut(s) 349, 779
BseMII CTCAG 3 cut(s) 453, 638, 762
BseNI ACTGG 5 cut(s) 407, 504, 639, 827, 892
BseRI GAGGAG 3 cut(s) 215, 218, 594
BseXI GCAGC 2 cut(s) 41, 132
Bsh1236I CGCG 1 cut(s) 110
BshFI GGCC 3 cut(s) 5, 182, 766
BsiSI CCGG 2 cut(s) 6, 315
BslFI GGGAC 1 cut(s) 417
BsmFI GGGAC 1 cut(s) 417
BsmI GAATGC 1 cut(s) 625
BsnI GGCC 3 cut(s) 5, 182, 766
Bsp1286I GDGCHC 2 cut(s) 74, 778
Bsp143I GATC 2 cut(s) 637, 935
Bsp1720I GCTNAGC 1 cut(s) 462
BspACI CCGC 4 cut(s) 104, 245, 545, 676
BspANI GGCC 3 cut(s) 5, 182, 766
BspCNI CTCAG 3 cut(s) 454, 637, 761
BspFNI CGCG 1 cut(s) 110
BspLI GGNNCC 4 cut(s) 405, 639, 777, 937
BspPI GGATC 4 cut(s) 632, 645, 930, 943
BsrDI GCAATG 2 cut(s) 349, 779
BsrI ACTGG 5 cut(s) 407, 504, 639, 827, 892
BssECI CCNNGG 2 cut(s) 771, 942
BssMI GATC 2 cut(s) 637, 935
BssNI GRCGYC 1 cut(s) 836
Bst2UI CCWGG 3 cut(s) 646, 772, 944
Bst6I CTCTTC 3 cut(s) 216, 374, 447
BstACI GRCGYC 1 cut(s) 836
BstAPI GCANNNNNTGC 1 cut(s) 620
BstC8I GCNNGC 3 cut(s) 108, 447, 768
BstDEI CTNAG 5 cut(s) 252, 462, 486, 624, 748
BstF5I GGATG 2 cut(s) 292, 463
BstFNI CGCG 1 cut(s) 110
BstHHI GCGC 1 cut(s) 468
BstKTI GATC 2 cut(s) 640, 938
BstMBI GATC 2 cut(s) 637, 935
BstMWI GCNNNNNNNGC 2 cut(s) 29, 620
BstNI CCWGG 3 cut(s) 646, 772, 944
BstNSI RCATGY 1 cut(s) 521
BstSCI CCNGG 4 cut(s) 313, 644, 770, 942
BstUI CGCG 1 cut(s) 110
BstV1I GCAGC 2 cut(s) 41, 132
BstV2I GAAGAC 3 cut(s) 68, 303, 930
BstX2I RGATCY 2 cut(s) 637, 935
BstYI RGATCY 2 cut(s) 637, 935
BsuRI GGCC 3 cut(s) 5, 182, 766
BtsCI GGATG 2 cut(s) 292, 463
BtsIMutI CAGTG 2 cut(s) 414, 756
Cac8I GCNNGC 3 cut(s) 108, 447, 768
CfoI GCGC 1 cut(s) 468
Cfr13I GGNCC 4 cut(s) 38, 180, 404, 764
CseI GACGC 2 cut(s) 99, 844
Csp6I GTAC 1 cut(s) 190
CviAII CATG 5 cut(s) 130, 518, 548, 796, 901
CviQI GTAC 1 cut(s) 190
DdeI CTNAG 5 cut(s) 252, 462, 486, 624, 748
DpnI GATC 2 cut(s) 639, 937
DpnII GATC 2 cut(s) 637, 935
DraI TTTAAA 1 cut(s) 652
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 216, 374, 447
EarI CTCTTC 3 cut(s) 216, 374, 447
EciI GGCGGA 1 cut(s) 260
Eco24I GRGCYC 2 cut(s) 74, 778
Eco47I GGWCC 2 cut(s) 38, 404
Eco57I CTGAAG 2 cut(s) 240, 471
EcoO109I RGGNCCY 1 cut(s) 764
EcoRII CCWGG 3 cut(s) 644, 770, 942
EcoT38I GRGCYC 2 cut(s) 74, 778
FaeI CATG 5 cut(s) 133, 521, 551, 799, 904
FaqI GGGAC 1 cut(s) 417
FatI CATG 5 cut(s) 129, 517, 547, 795, 900
FauI CCCGC 1 cut(s) 552
Fnu4HI GCNGC 3 cut(s) 30, 104, 121
FokI GGATG 2 cut(s) 299, 470
FriOI GRGCYC 2 cut(s) 74, 778
Fsp4HI GCNGC 3 cut(s) 30, 104, 121
FspBI CTAG 2 cut(s) 576, 714
GlaI GCGC 1 cut(s) 467
GluI GCNGC 3 cut(s) 30, 104, 121
HaeIII GGCC 3 cut(s) 5, 182, 766
HapII CCGG 2 cut(s) 6, 315
HgaI GACGC 2 cut(s) 99, 844
HhaI GCGC 1 cut(s) 468
Hin1I GRCGYC 1 cut(s) 836
Hin1II CATG 5 cut(s) 133, 521, 551, 799, 904
Hin6I GCGC 1 cut(s) 466
HinP1I GCGC 1 cut(s) 466
HincII GTYRAC 1 cut(s) 535
HindII GTYRAC 1 cut(s) 535
HindIII AAGCTT 2 cut(s) 164, 428
HinfI GANTC 1 cut(s) 194
HpaII CCGG 2 cut(s) 6, 315
Hpy166II GTNNAC 1 cut(s) 535
Hpy188I TCNGA 9 cut(s) 115, 199, 220, 286, 334, 627, 700, 735, 860
Hpy188III TCNNGA 2 cut(s) 364, 376
Hpy8I GTNNAC 1 cut(s) 535
HpyAV CCTTC 7 cut(s) 61, 221, 269, 651, 700, 755, 811
HpyCH4V TGCA 4 cut(s) 342, 437, 659, 725
HpyF10VI GCNNNNNNNGC 2 cut(s) 29, 620
HpyF3I CTNAG 5 cut(s) 252, 462, 486, 624, 748
Hsp92I GRCGYC 1 cut(s) 836
Hsp92II CATG 5 cut(s) 133, 521, 551, 799, 904
HspAI GCGC 1 cut(s) 466
Kzo9I GATC 2 cut(s) 637, 935
LmnI GCTCC 2 cut(s) 366, 781
Lsp1109I GCAGC 2 cut(s) 41, 132
LweI GCATC 3 cut(s) 90, 448, 646
MaeI CTAG 2 cut(s) 576, 714
MaeIII GTNAC 1 cut(s) 586
MalI GATC 2 cut(s) 639, 937
MboI GATC 2 cut(s) 637, 935
MfeI CAATTG 1 cut(s) 17
MflI RGATCY 2 cut(s) 637, 935
MhlI GDGCHC 2 cut(s) 74, 778
MluCI AATT 5 cut(s) 17, 318, 561, 790, 805
MluI ACGCGT 1 cut(s) 108
MroXI GAANNNNTTC 1 cut(s) 555
MseI TTAA 2 cut(s) 525, 651
MspI CCGG 2 cut(s) 6, 315
MspR9I CCNGG 4 cut(s) 315, 646, 772, 944
MunI CAATTG 1 cut(s) 17
Mva1269I GAATGC 1 cut(s) 625
MvaI CCWGG 3 cut(s) 646, 772, 944
MvnI CGCG 1 cut(s) 110
MwoI GCNNNNNNNGC 2 cut(s) 29, 620
NciI CCSGG 1 cut(s) 315
NdeII GATC 2 cut(s) 637, 935
NlaIII CATG 5 cut(s) 133, 521, 551, 799, 904
NlaIV GGNNCC 4 cut(s) 405, 639, 777, 937
NmuCI GTSAC 1 cut(s) 586
NspI RCATGY 1 cut(s) 521
PctI GAATGC 1 cut(s) 625
PdmI GAANNNNTTC 1 cut(s) 555
PfeI GAWTC 1 cut(s) 194
PkrI GCNGC 3 cut(s) 31, 105, 122
PsiI TTATAA 1 cut(s) 846
Psp6I CCWGG 3 cut(s) 644, 770, 942
PspGI CCWGG 3 cut(s) 644, 770, 942
PspN4I GGNNCC 4 cut(s) 405, 639, 777, 937
PspPI GGNCC 4 cut(s) 38, 180, 404, 764
PsuI RGATCY 2 cut(s) 637, 935
RsaI GTAC 1 cut(s) 191
RsaNI GTAC 1 cut(s) 190
SaqAI TTAA 2 cut(s) 525, 651
SatI GCNGC 3 cut(s) 30, 104, 121
Sau3AI GATC 2 cut(s) 637, 935
Sau96I GGNCC 4 cut(s) 38, 180, 404, 764
ScrFI CCNGG 4 cut(s) 315, 646, 772, 944
SduI GDGCHC 2 cut(s) 74, 778
SfaNI GCATC 3 cut(s) 90, 448, 646
SinI GGWCC 2 cut(s) 38, 404
SpeI ACTAGT 1 cut(s) 713
Sse9I AATT 5 cut(s) 17, 318, 561, 790, 805
SsiI CCGC 4 cut(s) 104, 245, 545, 676
SspMI CTAG 2 cut(s) 576, 714
StyD4I CCNGG 4 cut(s) 313, 644, 770, 942
TaqI TCGA 1 cut(s) 172
TasI AATT 5 cut(s) 17, 318, 561, 790, 805
TauI GCSGC 1 cut(s) 106
TfiI GAWTC 1 cut(s) 194
Tru1I TTAA 2 cut(s) 525, 651
Tru9I TTAA 2 cut(s) 525, 651
TscAI CASTG 2 cut(s) 414, 756
TseFI GTSAC 1 cut(s) 586
TseI GCWGC 2 cut(s) 29, 120
Tsp45I GTSAC 1 cut(s) 586
TspDTI ATGAA 7 cut(s) 146, 173, 318, 564, 774, 784, 917
TspRI CASTG 2 cut(s) 414, 756
VpaK11BI GGWCC 2 cut(s) 38, 404
XapI RAATTY 1 cut(s) 805
XceI RCATGY 1 cut(s) 521
XmnI GAANNNNTTC 1 cut(s) 555
XspI CTAG 2 cut(s) 576, 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.