MD17G1119200.v1.1

Rrp15p

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0009316
Physical Location & Seq
Forward (+)
10358109 .. 10359681
1573 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1119200.v1.1.491

Sequence Viewer

Length: 738 bp
ATGGCCGAAGAAACCCATCTGCCAGAAGCAGCAACAGAGCCAAGGAAGCGGAAAATAGGGAAGAAGAAAGGCTCGAAGGCAAAGAAGAAAGCGAAGATGTTACATTCGTCAGAGGAAATCAGAGCCATGAAGCCGAAGAAGTTTAACAATAAAATGAAGAAACTGTTTCTGAAACGAGCTAAGGACTACAATTCGGAGTCGGAGGAGGAGTCCAAACAGGAAGATGTGAAACCGAAACCGTTGGGAAACAATGAGAACGAGCCGATTGGCGGTGCCGCGTCGTCTTCAGGAGAAGAGTCGGAGGAAGGTGGTGGTGGTGGTGGTCGCAATAGGGGTGATACAGTTTCGGATGACGAGGAGCACGGCGAGATTCTGCCCGGAATCATGAAGTTTTCGGAGGGTTGCAATGCGTTTAGGTTGGCATTCAAGAGTATAATCAAGAAGTCAGTTTCTCTGAATGCAGTGGGACCCGTGATGTCGGCGCACAAGAAGCTTGTTGCAGAAAAGCTTGCCGAGGAGCAGACGGAGCGCAAGGCCAAGGGCGAGGCTAAGAAGGAAAAGCATACGGTGATAGAAAAAGGGCATGTGATGCCGGCCAATTATTTGGACACGCACGAAAAGTTTCTAATCGGTGTTGCTACTAAAGGAGTGGTGAAGTTGTTCAATGCTGTCAACAAGGCACAAAATGCTCAGAAAGGACTGAATCCATCCAAGTTTAAAGATGCCAAAGCCGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rrp15p

Protein Analysis

246

Amino Acids

26.81

Weight (kDa)

9.58

Isoelectric Point (pI)

47.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rrp15p PF07890 138 - 237 1.6e-18 Rrp15p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 272
AccII CGCG 1 cut(s) 278
AciI CCGC 3 cut(s) 49, 270, 276
AcoI YGGCCR 2 cut(s) 3, 594
AcuI CTGAAG 1 cut(s) 270
AfiI CCNNNNNNNGG 1 cut(s) 269
AgsI TTSAA 2 cut(s) 427, 664
AluBI AGCT 3 cut(s) 179, 493, 508
AluI AGCT 3 cut(s) 179, 493, 508
Alw21I GWGCWC 1 cut(s) 363
AoxI GGCC 3 cut(s) 3, 534, 594
ApeKI GCWGC 1 cut(s) 29
Asp700I GAANNNNTTC 2 cut(s) 621, 659
AspLEI GCGC 2 cut(s) 484, 531
AspS9I GGNCC 1 cut(s) 467
AsuC2I CCSGG 1 cut(s) 378
AsuHPI GGTGA 3 cut(s) 347, 580, 664
AvaII GGWCC 1 cut(s) 467
BanI GGYRCC 1 cut(s) 272
BbsI GAAGAC 1 cut(s) 276
Bbv12I GWGCWC 1 cut(s) 363
BbvI GCAGC 1 cut(s) 41
BccI CCATC 2 cut(s) 24, 715
BceAI ACGGC 2 cut(s) 379, 716
BcnI CCSGG 1 cut(s) 378
BisI GCNGC 2 cut(s) 30, 276
BlsI GCNGC 2 cut(s) 31, 277
Bme1390I CCNGG 1 cut(s) 378
Bme18I GGWCC 1 cut(s) 467
BmgT120I GGNCC 1 cut(s) 467
BmiI GGNNCC 3 cut(s) 274, 468, 469
BmrFI CCNGG 1 cut(s) 378
BmsI GCATC 2 cut(s) 579, 712
BpiI GAAGAC 1 cut(s) 276
Bpu10I CCTNAGC 1 cut(s) 180
BpuMI CCSGG 1 cut(s) 378
BsaJI CCNNGG 3 cut(s) 41, 513, 537
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bsc4I CCNNNNNNNGG 1 cut(s) 269
Bse118I RCCGGY 1 cut(s) 592
Bse3DI GCAATG 1 cut(s) 412
BseDI CCNNGG 3 cut(s) 41, 513, 537
BseGI GGATG 2 cut(s) 355, 707
BseLI CCNNNNNNNGG 1 cut(s) 269
BseMI GCAATG 1 cut(s) 412
BseMII CTCAG 1 cut(s) 704
BseRI GAGGAG 4 cut(s) 218, 221, 371, 530
BseXI GCAGC 1 cut(s) 41
Bsh1236I CGCG 1 cut(s) 278
BshFI GGCC 3 cut(s) 5, 536, 596
BshNI GGYRCC 1 cut(s) 272
BsiHKAI GWGCWC 1 cut(s) 363
BsiSI CCGG 2 cut(s) 378, 593
BslFI GGGAC 1 cut(s) 480
BslI CCNNNNNNNGG 1 cut(s) 269
BsmFI GGGAC 1 cut(s) 480
BsmI GAATGC 2 cut(s) 422, 463
BsnI GGCC 3 cut(s) 5, 536, 596
Bsp1286I GDGCHC 1 cut(s) 363
BspACI CCGC 3 cut(s) 49, 270, 276
BspANI GGCC 3 cut(s) 5, 536, 596
BspCNI CTCAG 1 cut(s) 703
BspFNI CGCG 1 cut(s) 278
BspHI TCATGA 1 cut(s) 384
BspLI GGNNCC 3 cut(s) 274, 468, 469
BspT107I GGYRCC 1 cut(s) 272
BsrDI GCAATG 1 cut(s) 412
BsrFI RCCGGY 1 cut(s) 592
BssAI RCCGGY 1 cut(s) 592
BssECI CCNNGG 3 cut(s) 41, 513, 537
BssT1I CCWWGG 2 cut(s) 41, 537
Bst4CI ACNGT 4 cut(s) 165, 240, 343, 568
Bst6I CTCTTC 1 cut(s) 288
BstAPI GCANNNNNTGC 2 cut(s) 589, 686
BstC8I GCNNGC 2 cut(s) 510, 594
BstDEI CTNAG 3 cut(s) 180, 549, 690
BstF5I GGATG 2 cut(s) 355, 707
BstFNI CGCG 1 cut(s) 278
BstHHI GCGC 2 cut(s) 484, 531
BstMWI GCNNNNNNNGC 5 cut(s) 46, 490, 526, 589, 686
BstNSI RCATGY 1 cut(s) 587
BstSCI CCNGG 1 cut(s) 376
BstUI CGCG 1 cut(s) 278
BstV1I GCAGC 1 cut(s) 41
BstV2I GAAGAC 1 cut(s) 276
BstXI CCANNNNNNTGG 1 cut(s) 604
BsuRI GGCC 3 cut(s) 5, 536, 596
BtsCI GGATG 2 cut(s) 355, 707
BtsI GCAGTG 1 cut(s) 468
BtsIMutI CAGTG 1 cut(s) 468
Cac8I GCNNGC 2 cut(s) 510, 594
CciI TCATGA 1 cut(s) 384
CfoI GCGC 2 cut(s) 484, 531
Cfr10I RCCGGY 1 cut(s) 592
Cfr13I GGNCC 1 cut(s) 467
CseI GACGC 1 cut(s) 267
CviAII CATG 3 cut(s) 127, 385, 584
DdeI CTNAG 3 cut(s) 180, 549, 690
DraI TTTAAA 1 cut(s) 718
EaeI YGGCCR 2 cut(s) 3, 594
Eam1104I CTCTTC 1 cut(s) 288
EarI CTCTTC 1 cut(s) 288
Eco130I CCWWGG 2 cut(s) 41, 537
Eco47I GGWCC 1 cut(s) 467
Eco57I CTGAAG 1 cut(s) 270
EcoO109I RGGNCCY 1 cut(s) 467
EcoT14I CCWWGG 2 cut(s) 41, 537
ErhI CCWWGG 2 cut(s) 41, 537
FaeI CATG 3 cut(s) 130, 388, 587
FaiI YATR 6 cut(s) 128, 386, 434, 564, 585, 736
FaqI GGGAC 1 cut(s) 480
FatI CATG 3 cut(s) 126, 384, 583
Fnu4HI GCNGC 2 cut(s) 30, 276
FokI GGATG 2 cut(s) 362, 694
Fsp4HI GCNGC 2 cut(s) 30, 276
GlaI GCGC 2 cut(s) 483, 530
GluI GCNGC 2 cut(s) 30, 276
HaeIII GGCC 3 cut(s) 5, 536, 596
HapII CCGG 2 cut(s) 378, 593
HgaI GACGC 1 cut(s) 267
HhaI GCGC 2 cut(s) 484, 531
Hin1II CATG 3 cut(s) 130, 388, 587
Hin6I GCGC 2 cut(s) 482, 529
HinP1I GCGC 2 cut(s) 482, 529
HincII GTYRAC 1 cut(s) 673
HindII GTYRAC 1 cut(s) 673
HindIII AAGCTT 2 cut(s) 491, 506
HinfI GANTC 6 cut(s) 197, 209, 296, 370, 381, 703
HpaII CCGG 2 cut(s) 378, 593
HphI GGTGA 3 cut(s) 347, 580, 664
Hpy166II GTNNAC 1 cut(s) 673
Hpy188III TCNNGA 4 cut(s) 288, 385, 427, 439
Hpy8I GTNNAC 1 cut(s) 673
Hpy99I CGWCG 1 cut(s) 283
HpyAV CCTTC 3 cut(s) 70, 299, 547
HpyCH4III ACNGT 4 cut(s) 165, 240, 343, 568
HpyCH4V TGCA 3 cut(s) 405, 461, 500
HpyF10VI GCNNNNNNNGC 5 cut(s) 46, 490, 526, 589, 686
HpyF3I CTNAG 3 cut(s) 180, 549, 690
Hsp92II CATG 3 cut(s) 130, 388, 587
HspAI GCGC 2 cut(s) 482, 529
KflI GGGWCCC 1 cut(s) 467
KroI GCCGGC 1 cut(s) 592
KroNI GCCGGC 1 cut(s) 594
LmnI GCTCC 3 cut(s) 358, 517, 526
LpnPI CCDG 5 cut(s) 36, 203, 273, 391, 606
Lsp1109I GCAGC 1 cut(s) 41
LweI GCATC 2 cut(s) 579, 712
MaeIII GTNAC 1 cut(s) 99
MhlI GDGCHC 1 cut(s) 363
MluCI AATT 2 cut(s) 190, 598
MlyI GAGTC 3 cut(s) 206, 218, 305
MmeI TCCRAC 2 cut(s) 180, 279
MnlI CCTC 8 cut(s) 106, 196, 199, 295, 349, 391, 508, 538
MroNI GCCGGC 1 cut(s) 592
MroXI GAANNNNTTC 2 cut(s) 621, 659
MseI TTAA 2 cut(s) 144, 717
MspI CCGG 2 cut(s) 378, 593
MspR9I CCNGG 1 cut(s) 378
Mva1269I GAATGC 2 cut(s) 422, 463
MvnI CGCG 1 cut(s) 278
MwoI GCNNNNNNNGC 5 cut(s) 46, 490, 526, 589, 686
NaeI GCCGGC 1 cut(s) 594
NciI CCSGG 1 cut(s) 378
NgoMIV GCCGGC 1 cut(s) 592
NlaIII CATG 3 cut(s) 130, 388, 587
NlaIV GGNNCC 3 cut(s) 274, 468, 469
NmeAIII GCCGAG 1 cut(s) 538
NspI RCATGY 1 cut(s) 587
PagI TCATGA 1 cut(s) 384
PctI GAATGC 2 cut(s) 422, 463
PdiI GCCGGC 1 cut(s) 594
PdmI GAANNNNTTC 2 cut(s) 621, 659
PfeI GAWTC 3 cut(s) 370, 381, 703
PkrI GCNGC 2 cut(s) 31, 277
PleI GAGTC 3 cut(s) 205, 217, 304
PpsI GAGTC 3 cut(s) 205, 217, 304
PpuMI RGGWCCY 1 cut(s) 467
Psp5II RGGWCCY 1 cut(s) 467
PspN4I GGNNCC 3 cut(s) 274, 468, 469
PspPI GGNCC 1 cut(s) 467
PspPPI RGGWCCY 1 cut(s) 467
SaqAI TTAA 2 cut(s) 144, 717
SatI GCNGC 2 cut(s) 30, 276
Sau96I GGNCC 1 cut(s) 467
SchI GAGTC 3 cut(s) 206, 218, 305
ScrFI CCNGG 1 cut(s) 378
SduI GDGCHC 1 cut(s) 363
SetI ASST 5 cut(s) 181, 310, 419, 495, 510
SfaNI GCATC 2 cut(s) 579, 712
SinI GGWCC 1 cut(s) 467
Sse9I AATT 2 cut(s) 190, 598
SsiI CCGC 3 cut(s) 49, 270, 276
StyD4I CCNGG 1 cut(s) 376
StyI CCWWGG 2 cut(s) 41, 537
TaaI ACNGT 4 cut(s) 165, 240, 343, 568
TaqI TCGA 1 cut(s) 74
TasI AATT 2 cut(s) 190, 598
TauI GCSGC 1 cut(s) 278
TfiI GAWTC 3 cut(s) 370, 381, 703
Tru1I TTAA 2 cut(s) 144, 717
Tru9I TTAA 2 cut(s) 144, 717
TscAI CASTG 1 cut(s) 468
TseI GCWGC 1 cut(s) 29
TspDTI ATGAA 3 cut(s) 143, 170, 401
TspGWI ACGGA 1 cut(s) 539
TspRI CASTG 1 cut(s) 468
VpaK11BI GGWCC 1 cut(s) 467
XceI RCATGY 1 cut(s) 587
XmnI GAANNNNTTC 2 cut(s) 621, 659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.