Rorug02G0448400

Rrp15p

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0009316
Physical Location & Seq
Reverse (-)
57546378 .. 57548053
1676 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0448400.1

Sequence Viewer

Length: 1113 bp
ATGGCAACTTCTTCAAGATTTCAAGTATATGTCCTGGCTTTTTGTGCAACCCTTCTTATTCAAAGTGGCTGTTATGGCCATTCTGTGCATCAGAGAAAACATGCAGCCTTGTTCATCTTCGGGGATTCAACATTTGATGTTGGAAATAATAACTACATAAACACTTCCACTTTCTTTCAAGCAAATTTCTTCCCATATGGGGAAACCTTCTTCAGCGACCCGACTGGTAGGTTCTCCGATGGTCGTCTAATCCCAGATATCATTGCTGAATATGCAAACTTGCCAATGATTCCACCATACTTACAGCCGGGTTTTGACAGCTATACTAATGGGGTGAACTTTGCATCTTCTGGGGCTGGTGTTCTAGCTGAAACTCATCAAGGATTTGTGTTGGACCTTAAAACTCAACTGGGTTATTTCAAAAATGTGGAGAAGCAGTTGAGGCACAGACTAGGTGAAGCAGAAGCTCACACATTGTTGTCCGAAGCTGTTTACTTGATTGCCATCGGAAGCGGTGATTACTCTTTCCCATTCATAGCGAATTCAAGTTTGTTCGAGTCTCACTCACATGAAGAATATGTTGGCATGGTGATAGGAAACCTTACAAATGTGATCAAAGAAATATACGAGAAAGGAGGAAGAAAATTTGGGATTAAAGGCATAGGGAATTTGGGTTGCATACCGGGCTTGAGAATAGTTAAACCAGGAAACACAAGCACCTGTAATGAAGAAGTAAATGCACTTTCAAAACTCCACAATAGAGTACTTGCTAAAGCCCTCCTGGAGCTGAAAGGACAGCTTCAAGACTTCGTATACTCGAATCCAGATTTATACTCTTACGCAAATGACGTAGTTCATAATCCATCAAAATATGGTTTCAAGGAAGCAAAGATGGCATGCTGTGGCTCTGGTCCATTAAGAAGAATTAGTAGCTGCGGAGGGAAGCGAGGTATGACTGAGTATGAGTTATGTGACAATGTTACTGACTATGTCTACTTTGATTCCGGCCATCCCACAGAAAGGTATAACCAGAAACTTTCCAAGTTATGGTGGAGCCATACTCCTGATGTCACAGGACGTTACATCAATTTGAAAGAGCTATTTGAAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rrp15p

Protein Analysis

370

Amino Acids

41.21

Weight (kDa)

6.46

Isoelectric Point (pI)

25.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 37 - 349 2.9e-25 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1047
AccI GTMKAC 2 cut(s) 813, 993
AciI CCGC 2 cut(s) 513, 936
AcoI YGGCCR 2 cut(s) 76, 1006
AcsI RAATTY 4 cut(s) 184, 541, 644, 667
AcuI CTGAAG 1 cut(s) 196
AfaI GTAC 1 cut(s) 765
AfiI CCNNNNNNNGG 3 cut(s) 199, 1020, 1047
AjnI CCWGG 3 cut(s) 33, 703, 780
AjuI GAANNNNNNNTTGG 2 cut(s) 564, 596
AluBI AGCT 8 cut(s) 321, 368, 467, 488, 787, 799, 933, 1099
AluI AGCT 8 cut(s) 321, 368, 467, 488, 787, 799, 933, 1099
Alw26I GTCTC 1 cut(s) 564
AoxI GGCC 2 cut(s) 76, 1006
ApeKI GCWGC 2 cut(s) 104, 933
ApoI RAATTY 4 cut(s) 184, 541, 644, 667
AspS9I GGNCC 2 cut(s) 394, 911
AsuC2I CCSGG 2 cut(s) 309, 684
AsuHPI GGTGA 4 cut(s) 346, 467, 527, 601
AvaII GGWCC 2 cut(s) 394, 911
BalI TGGCCA 1 cut(s) 78
BbvI GCAGC 2 cut(s) 116, 920
BccI CCATC 5 cut(s) 233, 512, 871, 886, 1017
BciT130I CCWGG 3 cut(s) 35, 705, 782
BclI TGATCA 1 cut(s) 612
BcnI CCSGG 2 cut(s) 309, 684
BcoDI GTCTC 1 cut(s) 564
BfaI CTAG 2 cut(s) 365, 452
BisI GCNGC 2 cut(s) 105, 934
BlsI GCNGC 2 cut(s) 106, 935
BmcAI AGTACT 1 cut(s) 765
Bme1390I CCNGG 5 cut(s) 35, 309, 684, 705, 782
Bme18I GGWCC 2 cut(s) 394, 911
BmgT120I GGNCC 2 cut(s) 394, 911
BmiI GGNNCC 1 cut(s) 1055
BmrFI CCNGG 5 cut(s) 35, 309, 684, 705, 782
BmrI ACTGGG 1 cut(s) 419
BmsI GCATC 2 cut(s) 97, 353
BmuI ACTGGG 1 cut(s) 419
BplI GAGNNNNNCTC 4 cut(s) 548, 580, 1045, 1077
BpmI CTGGAG 1 cut(s) 803
BpuEI CTTGAG 1 cut(s) 709
BpuMI CCSGG 2 cut(s) 309, 684
BsaBI GATNNNNATC 1 cut(s) 503
BsaXI ACNNNNNCTCC 2 cut(s) 422, 452
Bsc4I CCNNNNNNNGG 3 cut(s) 199, 1020, 1047
Bse1I ACTGG 2 cut(s) 229, 414
Bse3DI GCAATG 1 cut(s) 261
Bse8I GATNNNNATC 1 cut(s) 503
BseBI CCWGG 3 cut(s) 35, 705, 782
BseGI GGATG 1 cut(s) 1009
BseJI GATNNNNATC 1 cut(s) 503
BseLI CCNNNNNNNGG 3 cut(s) 199, 1020, 1047
BseMI GCAATG 1 cut(s) 261
BseMII CTCAG 1 cut(s) 948
BseNI ACTGG 2 cut(s) 229, 414
BseXI GCAGC 2 cut(s) 116, 920
BshFI GGCC 2 cut(s) 78, 1008
BsiSI CCGG 3 cut(s) 308, 683, 1005
BslI CCNNNNNNNGG 3 cut(s) 199, 1020, 1047
BsmAI GTCTC 1 cut(s) 564
BsnI GGCC 2 cut(s) 78, 1008
Bsp143I GATC 1 cut(s) 612
BspACI CCGC 2 cut(s) 513, 936
BspANI GGCC 2 cut(s) 78, 1008
BspCNI CTCAG 1 cut(s) 949
BspLI GGNNCC 1 cut(s) 1055
BsrDI GCAATG 1 cut(s) 261
BsrI ACTGG 2 cut(s) 229, 414
BssMI GATC 1 cut(s) 612
BssNAI GTATAC 1 cut(s) 814
Bst1107I GTATAC 1 cut(s) 814
Bst2UI CCWGG 3 cut(s) 35, 705, 782
BstC8I GCNNGC 1 cut(s) 898
BstDEI CTNAG 1 cut(s) 957
BstF5I GGATG 1 cut(s) 1009
BstKTI GATC 1 cut(s) 615
BstMAI GTCTC 1 cut(s) 564
BstMBI GATC 1 cut(s) 612
BstMWI GCNNNNNNNGC 6 cut(s) 44, 75, 272, 442, 684, 893
BstNI CCWGG 3 cut(s) 35, 705, 782
BstNSI RCATGY 2 cut(s) 104, 900
BstSCI CCNGG 5 cut(s) 33, 307, 682, 703, 780
BstV1I GCAGC 2 cut(s) 116, 920
BstZ17I GTATAC 1 cut(s) 814
BsuRI GGCC 2 cut(s) 78, 1008
BtsCI GGATG 1 cut(s) 1009
Cac8I GCNNGC 1 cut(s) 898
Cfr13I GGNCC 2 cut(s) 394, 911
Csp6I GTAC 1 cut(s) 764
CviAII CATG 4 cut(s) 101, 569, 586, 897
CviQI GTAC 1 cut(s) 764
DdeI CTNAG 1 cut(s) 957
DpnI GATC 1 cut(s) 614
DpnII GATC 1 cut(s) 612
EaeI YGGCCR 2 cut(s) 76, 1006
Eco32I GATATC 1 cut(s) 259
Eco47I GGWCC 2 cut(s) 394, 911
Eco57I CTGAAG 1 cut(s) 196
EcoRI GAATTC 1 cut(s) 541
EcoRII CCWGG 3 cut(s) 33, 703, 780
EcoRV GATATC 1 cut(s) 259
FaeI CATG 4 cut(s) 104, 572, 589, 900
FalI AAGNNNNNCTT 2 cut(s) 783, 815
FatI CATG 4 cut(s) 100, 568, 585, 896
FauNDI CATATG 1 cut(s) 196
FbaI TGATCA 1 cut(s) 612
FblI GTMKAC 2 cut(s) 813, 993
Fnu4HI GCNGC 2 cut(s) 105, 934
FokI GGATG 1 cut(s) 996
Fsp4HI GCNGC 2 cut(s) 105, 934
FspBI CTAG 2 cut(s) 365, 452
GluI GCNGC 2 cut(s) 105, 934
GsuI CTGGAG 1 cut(s) 803
HaeIII GGCC 2 cut(s) 78, 1008
HapII CCGG 3 cut(s) 308, 683, 1005
Hin1II CATG 4 cut(s) 104, 572, 589, 900
HinfI GANTC 5 cut(s) 125, 289, 557, 820, 1001
HpaII CCGG 3 cut(s) 308, 683, 1005
HphI GGTGA 4 cut(s) 346, 467, 527, 601
Hpy166II GTNNAC 4 cut(s) 337, 493, 814, 994
Hpy188I TCNGA 4 cut(s) 93, 238, 484, 509
Hpy188III TCNNGA 4 cut(s) 15, 803, 824, 1064
Hpy8I GTNNAC 4 cut(s) 337, 493, 814, 994
HpyAV CCTTC 2 cut(s) 62, 217
HpyCH4IV ACGT 2 cut(s) 849, 1078
HpyCH4V TGCA 7 cut(s) 47, 88, 104, 275, 344, 678, 740
HpyF10VI GCNNNNNNNGC 6 cut(s) 44, 75, 272, 442, 684, 893
HpyF3I CTNAG 1 cut(s) 957
HpySE526I ACGT 2 cut(s) 849, 1078
Hsp92II CATG 4 cut(s) 104, 572, 589, 900
Ksp22I TGATCA 1 cut(s) 612
Kzo9I GATC 1 cut(s) 612
LmnI GCTCC 2 cut(s) 784, 1053
Lsp1109I GCAGC 2 cut(s) 116, 920
LweI GCATC 2 cut(s) 97, 353
MaeI CTAG 2 cut(s) 365, 452
MaeII ACGT 2 cut(s) 849, 1078
MaeIII GTNAC 4 cut(s) 971, 979, 1069, 1079
MalI GATC 1 cut(s) 614
MboI GATC 1 cut(s) 612
MboII GAAGA 9 cut(s) 3, 109, 181, 202, 339, 584, 651, 740, 933
MlsI TGGCCA 1 cut(s) 78
MluCI AATT 6 cut(s) 184, 541, 644, 667, 924, 1087
MluNI TGGCCA 1 cut(s) 78
MlyI GAGTC 1 cut(s) 566
MmeI TCCRAC 2 cut(s) 121, 372
MnlI CCTC 5 cut(s) 435, 629, 788, 932, 941
Mox20I TGGCCA 1 cut(s) 78
MscI TGGCCA 1 cut(s) 78
MseI TTAA 4 cut(s) 399, 654, 699, 917
MslI CAYNNNNRTG 1 cut(s) 567
Msp20I TGGCCA 1 cut(s) 78
MspI CCGG 3 cut(s) 308, 683, 1005
MspR9I CCNGG 5 cut(s) 35, 309, 684, 705, 782
MvaI CCWGG 3 cut(s) 35, 705, 782
MwoI GCNNNNNNNGC 6 cut(s) 44, 75, 272, 442, 684, 893
NciI CCSGG 2 cut(s) 309, 684
NdeI CATATG 1 cut(s) 196
NdeII GATC 1 cut(s) 612
NlaIII CATG 4 cut(s) 104, 572, 589, 900
NlaIV GGNNCC 1 cut(s) 1055
NmuCI GTSAC 2 cut(s) 971, 1069
NspI RCATGY 2 cut(s) 104, 900
PaeI GCATGC 1 cut(s) 900
PcsI WCGNNNNNNNCGW 1 cut(s) 846
PfeI GAWTC 4 cut(s) 125, 289, 820, 1001
PflFI GACNNNGTC 1 cut(s) 989
PflMI CCANNNNNTGG 1 cut(s) 1047
PfoI TCCNGGA 1 cut(s) 780
PkrI GCNGC 2 cut(s) 106, 935
PleI GAGTC 1 cut(s) 565
PpsI GAGTC 1 cut(s) 565
Psp6I CCWGG 3 cut(s) 33, 703, 780
PspGI CCWGG 3 cut(s) 33, 703, 780
PspN4I GGNNCC 1 cut(s) 1055
PspPI GGNCC 2 cut(s) 394, 911
PsyI GACNNNGTC 1 cut(s) 989
RsaI GTAC 1 cut(s) 765
RsaNI GTAC 1 cut(s) 764
RseI CAYNNNNRTG 1 cut(s) 567
SaqAI TTAA 4 cut(s) 399, 654, 699, 917
SatI GCNGC 2 cut(s) 105, 934
Sau3AI GATC 1 cut(s) 612
Sau96I GGNCC 2 cut(s) 394, 911
ScaI AGTACT 1 cut(s) 765
SchI GAGTC 1 cut(s) 566
ScrFI CCNGG 5 cut(s) 35, 309, 684, 705, 782
SfaNI GCATC 2 cut(s) 97, 353
SinI GGWCC 2 cut(s) 394, 911
SmiMI CAYNNNNRTG 1 cut(s) 567
SmlI CTYRAG 1 cut(s) 688
SmoI CTYRAG 1 cut(s) 688
SphI GCATGC 1 cut(s) 900
Sse9I AATT 6 cut(s) 184, 541, 644, 667, 924, 1087
SsiI CCGC 2 cut(s) 513, 936
SspMI CTAG 2 cut(s) 365, 452
StyD4I CCNGG 5 cut(s) 33, 307, 682, 703, 780
TaiI ACGT 2 cut(s) 852, 1081
TaqI TCGA 2 cut(s) 555, 818
TasI AATT 6 cut(s) 184, 541, 644, 667, 924, 1087
TatI WGTACW 1 cut(s) 763
TfiI GAWTC 4 cut(s) 125, 289, 820, 1001
Tru1I TTAA 4 cut(s) 399, 654, 699, 917
Tru9I TTAA 4 cut(s) 399, 654, 699, 917
TseFI GTSAC 2 cut(s) 971, 1069
TseI GCWGC 2 cut(s) 104, 933
Tsp45I GTSAC 2 cut(s) 971, 1069
TspDTI ATGAA 5 cut(s) 103, 523, 585, 741, 845
Tth111I GACNNNGTC 1 cut(s) 989
Van91I CCANNNNNTGG 1 cut(s) 1047
VpaK11BI GGWCC 2 cut(s) 394, 911
XapI RAATTY 4 cut(s) 184, 541, 644, 667
XceI RCATGY 2 cut(s) 104, 900
XmiI GTMKAC 2 cut(s) 813, 993
XspI CTAG 2 cut(s) 365, 452
ZrmI AGTACT 1 cut(s) 765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.