Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0003474

Physical Location & Seq

Genomic Coordinates Reverse (-)

12520620 .. 12526155

Gene Structure 5536 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1157100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1542 bp

ATGTATAATAAGTCTCGTTTTGTCTTAAAATCTTTTGCCGGGTTCTGGCATCCAACGCCATCTCCTCCTCCACTCTTGACTCTATCTCTCTTCTGCTTTTCACAGACCCAAAGTGCTGGGCTCCATTTTCAGACAAACGTTCTCTCTCTCTCTCTCTCTCTGAAGGAGGCAAAGACTGAGGAGAAGTTGGTCCATTTGCTCAAATCCAATCCATTTTTGCTTCTGGGTTTGAGTGGGTTGATGGTTTCCATAGATGGAAAGACAGACCTTGAGTCTGATGAAGAAATCCCTTTTAGCTCATCAGCAGCCACCAGTAAAGTAGAGGCCTTGGACTTTGAGATGATTCATTTGCGGTCACGGGCAGATTTGGCGAACTCATGGGTTTCAACACATATGTGGATAAGAGTTCCAAGGAGTATTTATATAGTCTTATTTAAAGCAAAGATCAATGTGTTGCTACCCCTTGGTCCGTTGGCAATCTTGCTACATTATATTACTGGAAAGCATGGATGGGTCTTCTTCTTCAGCTTAGTGGGCATCGTACCTTTAGCAGAGCGTATAGGATATGTGACTGAGCAACTTGCTTTTTACACCGGACCAACAGTTGGGGGTCTGTTAAATGCCACATTTGGAAATGCAACGGAAATGATTATATCAATATATGCATTAAAGAACGGAATGATAAGGGTTGTTCAGCAGTCTTTATTGGGATCAATCTTGTCTAACATGCTCTTAGTGCTCGGATGTGCCTTCTTCACTGGTGGAATCATTCATTACCAAAAAATTCAGGTTTTCAACAAGTCTGCCGCCCTTGTGAATTCCGGATTGCTGTTGATGGCTGTCATGGGGTTAATGTTTCCAGCTGTGCTTCATTTTACGCACTCAGAGATCCACTTTGGGAAGTCAGAGTTGTCTCTTTCAAGATTTAGCAGCTGTGTAATGCTAGTGGCATATGCAAGTTACCTCTTCTTTCAACTTAGAAGTCATCGTAATCTTTATAATCCCATTGAGGAGGAAGGAATCAGGGATGAAGATGAATCCGATGAAGAAGAGGCTCCCGAGATAACTCATTGGGAAGCAATTGGCTGGCTTGCTGTTTTAACCGTCTGGGTGTCCGTTTTGTCTGGATACCTTGTTGATGCTATCCAGGGAGCATCTGACTCATTCAACATGCCTGTGGCATTTATCAGTGTTATACTACTACCAATTGTAGGAAATGCTGCAGAGCATGCAAGTGCTATCATGTTTGCCATGAAGGACAAGCTTGATATCACACTTGGAGTTGCAATTGGATCATCTACACAAATATCCATGTTTGTGATACCCTTCTGCGTAGTTGTGGGGTGGATCATGGGACTGCCAATGGACTTGAATTTTCAACTGTTCGAAACTGCCACACTTTTTATTACAGTGTTAGTCGTGGCATTTATGTTGCAGGATGGGACGTCAAACTATTTTAAAGGCTTGATGCTTATTCTGTGCTATCTCATAGTTGCAGCCAGTTTTTTTGTGCACGTCGATCCTTCAGAAGATGGTAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0009705 GO:0016020 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_Ca_ex

Protein Analysis

514

Amino Acids

56.6

Weight (kDa)

5.58

Isoelectric Point (pI)

36.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_Ca_ex	PF01699	171 - 326	4e-22	Sodium/calcium exchanger protein
Na_Ca_ex	PF01699	360 - 502	4e-23	Sodium/calcium exchanger protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003474)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g37510 FvH4_6g37510
malus_domestica	MD09G1157100.v1.1 MD17G1143500.v1.1
prunus_persica	Prupe.3G168500_v2.0.a1 Prupe.3G168500_v2.0.a1 Prupe.3G168500_v2.0.a1 Prupe.3G168500_v2.0.a1 Prupe.3G168500_v2.0.a1 Prupe.3G168500_v2.0.a1 Prupe.3G168500_v2.0.a1 Prupe.3G168500_v2.0.a1 Prupe.3G168500_v2.0.a1
pyrus_communis	pycom09g07580 pycom09g07590 pycom17g13660
rosa_chinensis	RchiOBHm_Chr2g0150371 RchiOBHm_Chr2g0150391 RchiOBHm_Chr7g0199401
rosa_laevigata	RLG00000020493 RLG00000020495
rosa_multiflora	Rmu_sc0002179.1_g000009 Rmu_ssc0000480.1_g000006
rosa_roxburghii	Rroxscaffold_2G00097160 Rroxscaffold_2G00097180
rosa_rugosa	Rorug02G0421000 Rorug02G0421000 Rorug02G0421000 Rorug02G0421100
rosa_samantha	Rh2AG480100 Rh2AG480300 Rh2CG466400 Rh2CG466600 Rh2DG501100 Rh5DG402100
rosa_wichuraiana	Rw2G039180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	999
AatII	GACGTC	1 cut(s)	1448
AccB7I	CCANNNNNTGG	1 cut(s)	605
AccIII	TCCGGA	1 cut(s)	821
AciI	CCGC	2 cut(s)	352, 807
AclI	AACGTT	1 cut(s)	138
AclWI	GGATC	5 cut(s)	718, 883, 1300, 1355, 1514
AcsI	RAATTY	3 cut(s)	783, 817, 1372
AcuI	CTGAAG	3 cut(s)	182, 508, 1509
AcyI	GRCGYC	1 cut(s)	1445
AfaI	GTAC	1 cut(s)	543
AfiI	CCNNNNNNNGG	3 cut(s)	45, 605, 1211
AgsI	TTSAA	7 cut(s)	387, 796, 921, 974, 1168, 1372, 1379
AhdI	GACNNNNNGTC	1 cut(s)	271
AjiI	CACGTC	1 cut(s)	1516
AjnI	CCWGG	1 cut(s)	1146
AleI	CACNNNNGTG	1 cut(s)	394
AluBI	AGCT	5 cut(s)	297, 528, 863, 933, 1264
AluI	AGCT	5 cut(s)	297, 528, 863, 933, 1264
Alw21I	GWGCWC	2 cut(s)	741, 1515
Alw26I	GTCTC	2 cut(s)	18, 918
Alw44I	GTGCAC	1 cut(s)	1511
AlwI	GGATC	5 cut(s)	718, 883, 1300, 1355, 1514
Ama87I	CYCGRG	1 cut(s)	1058
Aor13HI	TCCGGA	1 cut(s)	821
AoxI	GGCC	1 cut(s)	324
ApaLI	GTGCAC	1 cut(s)	1511
ApeKI	GCWGC	4 cut(s)	305, 930, 1220, 1496
ApoI	RAATTY	3 cut(s)	783, 817, 1372
AspS9I	GGNCC	3 cut(s)	190, 467, 596
AsuC2I	CCSGG	1 cut(s)	40
AsuII	TTCGAA	1 cut(s)	1386
AvaI	CYCGRG	1 cut(s)	1058
AvaII	GGWCC	3 cut(s)	190, 467, 596
BaeGI	GKGCMC	1 cut(s)	1515
BanII	GRGCYC	1 cut(s)	123
BbsI	GAAGAC	1 cut(s)	508
Bbv12I	GWGCWC	2 cut(s)	741, 1515
BbvI	GCAGC	4 cut(s)	317, 942, 1207, 1508
BccI	CCATC	7 cut(s)	67, 235, 248, 504, 829, 1433, 1526
BciT130I	CCWGG	1 cut(s)	1148
BciVI	GTATCC	1 cut(s)	1121
BcnI	CCSGG	1 cut(s)	40
BcoDI	GTCTC	2 cut(s)	18, 918
BfaI	CTAG	1 cut(s)	944
BfmI	CTRYAG	1 cut(s)	1221
BfuI	GTATCC	1 cut(s)	1121
BisI	GCNGC	5 cut(s)	306, 807, 931, 1221, 1497
BlsI	GCNGC	5 cut(s)	307, 808, 932, 1222, 1498
Bme1390I	CCNGG	2 cut(s)	40, 1148
Bme18I	GGWCC	3 cut(s)	190, 467, 596
BmeRI	GACNNNNNGTC	1 cut(s)	271
BmeT110I	CYCGRG	1 cut(s)	1058
BmgBI	CACGTC	1 cut(s)	1516
BmgT120I	GGNCC	3 cut(s)	190, 467, 596
BmiI	GGNNCC	2 cut(s)	122, 1056
BmrFI	CCNGG	2 cut(s)	40, 1148
BmsI	GCATC	5 cut(s)	58, 546, 1129, 1163, 1458
BpiI	GAAGAC	1 cut(s)	508
Bpu14I	TTCGAA	1 cut(s)	1386
BpuEI	CTTGAG	1 cut(s)	290
BpuMI	CCSGG	1 cut(s)	40
BsaHI	GRCGYC	1 cut(s)	1445
BsaJI	CCNNGG	4 cut(s)	327, 410, 463, 1147
BsaWI	WCCGGW	2 cut(s)	593, 821
BsaXI	ACNNNNNCTCC	2 cut(s)	46, 76
Bsc4I	CCNNNNNNNGG	3 cut(s)	45, 605, 1211
Bse1I	ACTGG	4 cut(s)	312, 502, 763, 1500
BseAI	TCCGGA	1 cut(s)	821
BseBI	CCWGG	1 cut(s)	1148
BseDI	CCNNGG	4 cut(s)	327, 410, 463, 1147
BseGI	GGATG	5 cut(s)	49, 515, 749, 1033, 1444
BseLI	CCNNNNNNNGG	3 cut(s)	45, 605, 1211
BseMII	CTCAG	3 cut(s)	168, 564, 897
BseNI	ACTGG	4 cut(s)	312, 502, 763, 1500
BseRI	GAGGAG	4 cut(s)	54, 57, 194, 1025
BseSI	GKGCMC	1 cut(s)	1515
BseXI	GCAGC	4 cut(s)	317, 942, 1207, 1508
BseYI	CCCAGC	1 cut(s)	116
BshFI	GGCC	1 cut(s)	326
BsiHKAI	GWGCWC	2 cut(s)	741, 1515
BsiHKCI	CYCGRG	1 cut(s)	1058
BsiSI	CCGG	3 cut(s)	39, 594, 822
BslFI	GGGAC	2 cut(s)	1368, 1456
BslI	CCNNNNNNNGG	3 cut(s)	45, 605, 1211
BsmAI	GTCTC	2 cut(s)	18, 918
BsmFI	GGGAC	2 cut(s)	1368, 1456
BsnI	GGCC	1 cut(s)	326
BsoBI	CYCGRG	1 cut(s)	1058
Bsp119I	TTCGAA	1 cut(s)	1386
Bsp1286I	GDGCHC	3 cut(s)	123, 741, 1515
Bsp13I	TCCGGA	1 cut(s)	821
Bsp143I	GATC	6 cut(s)	444, 710, 888, 1292, 1347, 1519
BspACI	CCGC	2 cut(s)	352, 807
BspANI	GGCC	1 cut(s)	326
BspCNI	CTCAG	3 cut(s)	169, 565, 896
BspEI	TCCGGA	1 cut(s)	821
BspLI	GGNNCC	2 cut(s)	122, 1056
BspMAI	CTGCAG	1 cut(s)	1225
BspPI	GGATC	5 cut(s)	718, 883, 1300, 1355, 1514
BspT104I	TTCGAA	1 cut(s)	1386
BsrI	ACTGG	4 cut(s)	312, 502, 763, 1500
BssECI	CCNNGG	4 cut(s)	327, 410, 463, 1147
BssMI	GATC	6 cut(s)	444, 710, 888, 1292, 1347, 1519
BssNI	GRCGYC	1 cut(s)	1445
BssT1I	CCWWGG	3 cut(s)	327, 410, 463
Bst2UI	CCWGG	1 cut(s)	1148
Bst4CI	ACNGT	4 cut(s)	604, 1105, 1383, 1411
Bst6I	CTCTTC	3 cut(s)	95, 971, 1044
BstACI	GRCGYC	1 cut(s)	1445
BstAPI	GCANNNNNTGC	1 cut(s)	1229
BstBI	TTCGAA	1 cut(s)	1386
BstC8I	GCNNGC	3 cut(s)	1088, 1092, 1230
BstDEI	CTNAG	6 cut(s)	177, 529, 573, 733, 883, 977
BstF5I	GGATG	5 cut(s)	49, 515, 749, 1033, 1444
BstKTI	GATC	6 cut(s)	447, 713, 891, 1295, 1350, 1522
BstMAI	GTCTC	2 cut(s)	18, 918
BstMBI	GATC	6 cut(s)	444, 710, 888, 1292, 1347, 1519
BstMWI	GCNNNNNNNGC	5 cut(s)	55, 368, 534, 736, 1229
BstNI	CCWGG	1 cut(s)	1148
BstNSI	RCATGY	3 cut(s)	730, 1174, 1232
BstSCI	CCNGG	2 cut(s)	38, 1146
BstSFI	CTRYAG	1 cut(s)	1221
BstSLI	GKGCMC	1 cut(s)	1515
BstV1I	GCAGC	4 cut(s)	317, 942, 1207, 1508
BstV2I	GAAGAC	1 cut(s)	508
BstX2I	RGATCY	1 cut(s)	888
BstXI	CCANNNNNNTGG	1 cut(s)	116
BstYI	RGATCY	1 cut(s)	888
BsuI	GTATCC	1 cut(s)	1121
BsuRI	GGCC	1 cut(s)	326
BtrI	CACGTC	1 cut(s)	1516
BtsCI	GGATG	5 cut(s)	49, 515, 749, 1033, 1444
BtsIMutI	CAGTG	3 cut(s)	756, 1195, 1416
Cac8I	GCNNGC	3 cut(s)	1088, 1092, 1230
Cfr13I	GGNCC	3 cut(s)	190, 467, 596
Csp6I	GTAC	1 cut(s)	542
CviQI	GTAC	1 cut(s)	542
DdeI	CTNAG	6 cut(s)	177, 529, 573, 733, 883, 977
DpnI	GATC	6 cut(s)	446, 712, 890, 1294, 1349, 1521
DpnII	GATC	6 cut(s)	444, 710, 888, 1292, 1347, 1519
DraI	TTTAAA	2 cut(s)	436, 1459
DriI	GACNNNNNGTC	1 cut(s)	271
Eam1104I	CTCTTC	3 cut(s)	95, 971, 1044
Eam1105I	GACNNNNNGTC	1 cut(s)	271
EarI	CTCTTC	3 cut(s)	95, 971, 1044
Eco130I	CCWWGG	3 cut(s)	327, 410, 463
Eco147I	AGGCCT	1 cut(s)	326
Eco24I	GRGCYC	1 cut(s)	123
Eco32I	GATATC	1 cut(s)	1270
Eco47I	GGWCC	3 cut(s)	190, 467, 596
Eco57I	CTGAAG	3 cut(s)	182, 508, 1509
Eco88I	CYCGRG	1 cut(s)	1058
EcoRI	GAATTC	1 cut(s)	817
EcoRII	CCWGG	1 cut(s)	1146
EcoRV	GATATC	1 cut(s)	1270
EcoT14I	CCWWGG	3 cut(s)	327, 410, 463
EcoT22I	ATGCAT	1 cut(s)	667
EcoT38I	GRGCYC	1 cut(s)	123
ErhI	CCWWGG	3 cut(s)	327, 410, 463
FaqI	GGGAC	2 cut(s)	1368, 1456
FauNDI	CATATG	2 cut(s)	393, 952
Fnu4HI	GCNGC	5 cut(s)	306, 807, 931, 1221, 1497
FokI	GGATG	5 cut(s)	36, 522, 756, 1040, 1451
FriOI	GRGCYC	1 cut(s)	123
Fsp4HI	GCNGC	5 cut(s)	306, 807, 931, 1221, 1497
FspBI	CTAG	1 cut(s)	944
GluI	GCNGC	5 cut(s)	306, 807, 931, 1221, 1497
GsaI	CCCAGC	1 cut(s)	120
HaeIII	GGCC	1 cut(s)	326
HapII	CCGG	3 cut(s)	39, 594, 822
Hin1I	GRCGYC	1 cut(s)	1445
HindIII	AAGCTT	1 cut(s)	1262
HinfI	GANTC	7 cut(s)	79, 272, 343, 765, 1020, 1037, 1160
HpaII	CCGG	3 cut(s)	39, 594, 822
Hpy166II	GTNNAC	1 cut(s)	1513
Hpy188I	TCNGA	9 cut(s)	132, 162, 277, 743, 886, 907, 1042, 1159, 1528
Hpy188III	TCNNGA	5 cut(s)	76, 822, 921, 1058, 1125
Hpy8I	GTNNAC	1 cut(s)	1513
Hpy99I	CGWCG	1 cut(s)	1520
HpyAV	CCTTC	6 cut(s)	157, 760, 1010, 1249, 1336, 1533
HpyCH4III	ACNGT	4 cut(s)	604, 1105, 1383, 1411
HpyCH4IV	ACGT	3 cut(s)	138, 1445, 1515
HpyCH4V	TGCA	9 cut(s)	638, 665, 956, 1223, 1232, 1286, 1435, 1496, 1513
HpyF10VI	GCNNNNNNNGC	5 cut(s)	55, 368, 534, 736, 1229
HpyF3I	CTNAG	6 cut(s)	177, 529, 573, 733, 883, 977
HpySE526I	ACGT	3 cut(s)	138, 1445, 1515
Hsp92I	GRCGYC	1 cut(s)	1445
Kpn2I	TCCGGA	1 cut(s)	821
Kzo9I	GATC	6 cut(s)	444, 710, 888, 1292, 1347, 1519
LmnI	GCTCC	3 cut(s)	126, 1060, 1151
Lsp1109I	GCAGC	4 cut(s)	317, 942, 1207, 1508
LweI	GCATC	5 cut(s)	58, 546, 1129, 1163, 1458
MaeI	CTAG	1 cut(s)	944
MaeII	ACGT	3 cut(s)	138, 1445, 1515
MaeIII	GTNAC	3 cut(s)	354, 568, 959
MalI	GATC	6 cut(s)	446, 712, 890, 1294, 1349, 1521
MboI	GATC	6 cut(s)	444, 710, 888, 1292, 1347, 1519
MfeI	CAATTG	3 cut(s)	1080, 1206, 1287
MflI	RGATCY	1 cut(s)	888
MhlI	GDGCHC	3 cut(s)	123, 741, 1515
MluCI	AATT	7 cut(s)	783, 817, 1080, 1206, 1287, 1372, 1537
MlyI	GAGTC	3 cut(s)	73, 281, 1154
MmeI	TCCRAC	1 cut(s)	77
MnlI	CCTC	9 cut(s)	75, 78, 160, 172, 316, 974, 1003, 1006, 1045
Mph1103I	ATGCAT	1 cut(s)	667
MroI	TCCGGA	1 cut(s)	821
MseI	TTAA	8 cut(s)	26, 435, 617, 668, 851, 1100, 1458, 1540
MslI	CAYNNNNRTG	4 cut(s)	394, 1175, 1233, 1316
MspA1I	CMGCKG	2 cut(s)	863, 933
MspI	CCGG	3 cut(s)	39, 594, 822
MspR9I	CCNGG	2 cut(s)	40, 1148
MunI	CAATTG	3 cut(s)	1080, 1206, 1287
MvaI	CCWGG	1 cut(s)	1148
MwoI	GCNNNNNNNGC	5 cut(s)	55, 368, 534, 736, 1229
NciI	CCSGG	1 cut(s)	40
NdeI	CATATG	2 cut(s)	393, 952
NdeII	GATC	6 cut(s)	444, 710, 888, 1292, 1347, 1519
NlaIV	GGNNCC	2 cut(s)	122, 1056
NmuCI	GTSAC	2 cut(s)	354, 568
NsiI	ATGCAT	1 cut(s)	667
NspI	RCATGY	3 cut(s)	730, 1174, 1232
NspV	TTCGAA	1 cut(s)	1386
OliI	CACNNNNGTG	1 cut(s)	394
PaeI	GCATGC	1 cut(s)	1232
PceI	AGGCCT	1 cut(s)	326
PfeI	GAWTC	4 cut(s)	343, 765, 1020, 1037
PflMI	CCANNNNNTGG	1 cut(s)	605
PkrI	GCNGC	5 cut(s)	307, 808, 932, 1222, 1498
PleI	GAGTC	3 cut(s)	73, 280, 1154
PpsI	GAGTC	3 cut(s)	73, 280, 1154
PsiI	TTATAA	1 cut(s)	999
Psp1406I	AACGTT	1 cut(s)	138
Psp6I	CCWGG	1 cut(s)	1146
PspFI	CCCAGC	1 cut(s)	116
PspGI	CCWGG	1 cut(s)	1146
PspN4I	GGNNCC	2 cut(s)	122, 1056
PspPI	GGNCC	3 cut(s)	190, 467, 596
PstI	CTGCAG	1 cut(s)	1225
PsuI	RGATCY	1 cut(s)	888
PvuII	CAGCTG	2 cut(s)	863, 933
RsaI	GTAC	1 cut(s)	543
RsaNI	GTAC	1 cut(s)	542
RseI	CAYNNNNRTG	4 cut(s)	394, 1175, 1233, 1316
SaqAI	TTAA	8 cut(s)	26, 435, 617, 668, 851, 1100, 1458, 1540
SatI	GCNGC	5 cut(s)	306, 807, 931, 1221, 1497
Sau3AI	GATC	6 cut(s)	444, 710, 888, 1292, 1347, 1519
Sau96I	GGNCC	3 cut(s)	190, 467, 596
SchI	GAGTC	3 cut(s)	73, 281, 1154
ScrFI	CCNGG	2 cut(s)	40, 1148
SduI	GDGCHC	3 cut(s)	123, 741, 1515
SfaNI	GCATC	5 cut(s)	58, 546, 1129, 1163, 1458
SfcI	CTRYAG	1 cut(s)	1221
SfuI	TTCGAA	1 cut(s)	1386
SinI	GGWCC	3 cut(s)	190, 467, 596
SmiMI	CAYNNNNRTG	4 cut(s)	394, 1175, 1233, 1316
SmlI	CTYRAG	1 cut(s)	269
SmoI	CTYRAG	1 cut(s)	269
SphI	GCATGC	1 cut(s)	1232
Sse9I	AATT	7 cut(s)	783, 817, 1080, 1206, 1287, 1372, 1537
SseBI	AGGCCT	1 cut(s)	326
SsiI	CCGC	2 cut(s)	352, 807
SspMI	CTAG	1 cut(s)	944
StuI	AGGCCT	1 cut(s)	326
StyD4I	CCNGG	2 cut(s)	38, 1146
StyI	CCWWGG	3 cut(s)	327, 410, 463
TaaI	ACNGT	4 cut(s)	604, 1105, 1383, 1411
TaiI	ACGT	3 cut(s)	141, 1448, 1518
TaqI	TCGA	2 cut(s)	1386, 1518
TasI	AATT	7 cut(s)	783, 817, 1080, 1206, 1287, 1372, 1537
TauI	GCSGC	1 cut(s)	809
TfiI	GAWTC	4 cut(s)	343, 765, 1020, 1037
Tru1I	TTAA	8 cut(s)	26, 435, 617, 668, 851, 1100, 1458, 1540
Tru9I	TTAA	8 cut(s)	26, 435, 617, 668, 851, 1100, 1458, 1540
TscAI	CASTG	3 cut(s)	763, 1195, 1416
TseFI	GTSAC	2 cut(s)	354, 568
TseI	GCWGC	4 cut(s)	305, 930, 1220, 1496
Tsp45I	GTSAC	2 cut(s)	354, 568
TspDTI	ATGAA	8 cut(s)	294, 335, 761, 860, 1044, 1050, 1059, 1268
TspGWI	ACGGA	4 cut(s)	459, 656, 690, 1105
TspRI	CASTG	3 cut(s)	763, 1195, 1416
Van91I	CCANNNNNTGG	1 cut(s)	605
VneI	GTGCAC	1 cut(s)	1511
VpaK11BI	GGWCC	3 cut(s)	190, 467, 596
XapI	RAATTY	3 cut(s)	783, 817, 1372
XceI	RCATGY	3 cut(s)	730, 1174, 1232
XspI	CTAG	1 cut(s)	944
ZraI	GACGTC	1 cut(s)	1446
Zsp2I	ATGCAT	1 cut(s)	667

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.