Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0003474

Physical Location & Seq

Genomic Coordinates Reverse (-)

12932234 .. 12937843

Gene Structure 5610 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1143500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1203 bp

ATGATTCATTTGCGGTCACGAGCAGATTTGGCGAAGTCATGGGTTTCAAGACATATGTGGATAAGAGTTCCGAGGAGTATTTATATAGTCTTATTTAAAGCAAAGATCAATATGTTGCTACCCTTTGGTCCTTTGGCGATTTTGCTACATTATATTACTGGAAAGCATGGATGGGTATTCTTCTTCAGCTTAGTGGGCATCGTACCTTTAGCAGAGCGTCTAGGATATGCCACTGAGCAACTTGCTTTCTACACTGGACCAACAGTTGGGGGTCTGTTAAATGCCACATTTGGAAATGCCACAGAAATGATTATATCAATATATGCATTAAAGAACGGAATGATAAGGGTTGTTCAGCAATCTTTATTAGGATCAATCTTGTCCAACATGCTTTTAGTGCTCGGATGTGCCTTCTTCACTGGTGGAATCATTCATTACCAAAAAATTCAGGTTTTCAACAAGTCTGCAGCCCTCGTGAATTCAGGATTGCTGTTGATGGCTGTCATGGGGTTAATGTTTCCGGCTGTGCTTCATTTTACGCGCTCAGAGATCCACTTTGGGAAGTCAGAGTTATCTCTTTCAAGATTTAGCAGCTGTGTAATGCTAGTGGCATATGCGAGCTACCTTTTCTTTCAACTTAGAAGTCATCGTAATCTTTATAATCCCATTGAGGAGGAAGGAGTCAGGGGTGAAGATGAATCCGATGAGGAAGAGGCTCCCGAGATAACTCATTGGGAAGCAATTGGCTGGCTTGCTGTTTTAACTGTCTGGGTGTCCGTTTTGTCTGGATACCTTGTGGATGCTATCCAGGGAGCGTCTGACTCATTCAACATGCCTGTGGCATTTATCAGTGTTATACTACTACCAATTGTAGGAAATGCTGCAGAGCATGCAAGTGCTATCATGTTTGCCATGAAGGACAAGCTTGATATCACACTCGGAGTTGCAATTGGATCATCTACACAAATATCCATGTTTGTGATACCCTTCTGCGTAGTTGTTGGGTGGATCATGGGACAGCCAATGGACTTGAATTTTCAACTGTTTGAGACTGCCACACTTTTTATTACAGTGTTAGTCGTGGCATTTATGTTGCAGGAAGGGACGTCAAACTACTTTAAAGGCTTGATGCTTATTCTGTGCTATCTCATAGTTGCCGCCAGTTTTTTTGTACACGTCGATCCTTCAGATGATGGTAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0009705 GO:0016020 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_Ca_ex

Protein Analysis

401

Amino Acids

44.25

Weight (kDa)

5.99

Isoelectric Point (pI)

37.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_Ca_ex	PF01699	58 - 213	3.6e-23	Sodium/calcium exchanger protein
Na_Ca_ex	PF01699	247 - 389	2.4e-24	Sodium/calcium exchanger protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003474)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g37510 FvH4_6g37510
malus_domestica	MD09G1157100.v1.1 MD17G1143500.v1.1
prunus_persica	Prupe.3G168500_v2.0.a1 Prupe.3G168500_v2.0.a1 Prupe.3G168500_v2.0.a1 Prupe.3G168500_v2.0.a1 Prupe.3G168500_v2.0.a1 Prupe.3G168500_v2.0.a1 Prupe.3G168500_v2.0.a1 Prupe.3G168500_v2.0.a1 Prupe.3G168500_v2.0.a1
pyrus_communis	pycom09g07580 pycom09g07590 pycom17g13660
rosa_chinensis	RchiOBHm_Chr2g0150371 RchiOBHm_Chr2g0150391 RchiOBHm_Chr7g0199401
rosa_laevigata	RLG00000020493 RLG00000020495
rosa_multiflora	Rmu_sc0002179.1_g000009 Rmu_ssc0000480.1_g000006
rosa_roxburghii	Rroxscaffold_2G00097160 Rroxscaffold_2G00097180
rosa_rugosa	Rorug02G0421000 Rorug02G0421000 Rorug02G0421000 Rorug02G0421100
rosa_samantha	Rh2AG480100 Rh2AG480300 Rh2CG466400 Rh2CG466600 Rh2DG501100 Rh5DG402100
rosa_wichuraiana	Rw2G039180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	660
AatII	GACGTC	1 cut(s)	1109
AccB7I	CCANNNNNTGG	1 cut(s)	266
AccII	CGCG	1 cut(s)	541
AciI	CCGC	2 cut(s)	13, 1158
AclWI	GGATC	5 cut(s)	379, 544, 961, 1016, 1175
AcsI	RAATTY	3 cut(s)	444, 478, 1033
AcuI	CTGAAG	2 cut(s)	169, 1170
AcyI	GRCGYC	1 cut(s)	1106
AfaI	GTAC	2 cut(s)	204, 1173
AfiI	CCNNNNNNNGG	2 cut(s)	266, 872
AflIII	ACRYGT	1 cut(s)	1174
AgsI	TTSAA	7 cut(s)	48, 457, 582, 635, 829, 1033, 1040
AjiI	CACGTC	1 cut(s)	1177
AjnI	CCWGG	1 cut(s)	807
AluBI	AGCT	4 cut(s)	189, 594, 621, 925
AluI	AGCT	4 cut(s)	189, 594, 621, 925
Alw21I	GWGCWC	1 cut(s)	402
Alw26I	GTCTC	1 cut(s)	1043
AlwI	GGATC	5 cut(s)	379, 544, 961, 1016, 1175
Ama87I	CYCGRG	1 cut(s)	719
ApeKI	GCWGC	3 cut(s)	467, 591, 881
ApoI	RAATTY	3 cut(s)	444, 478, 1033
AspLEI	GCGC	1 cut(s)	543
AspS9I	GGNCC	2 cut(s)	128, 257
AsuHPI	GGTGA	1 cut(s)	701
AvaI	CYCGRG	1 cut(s)	719
AvaII	GGWCC	2 cut(s)	128, 257
BauI	CACGAG	2 cut(s)	18, 473
Bbv12I	GWGCWC	1 cut(s)	402
BbvI	GCAGC	3 cut(s)	479, 603, 868
BccI	CCATC	3 cut(s)	165, 490, 1187
BciT130I	CCWGG	1 cut(s)	809
BciVI	GTATCC	1 cut(s)	782
BcoDI	GTCTC	1 cut(s)	1043
BfaI	CTAG	2 cut(s)	221, 605
BfmI	CTRYAG	2 cut(s)	465, 882
BfuI	GTATCC	1 cut(s)	782
BisI	GCNGC	4 cut(s)	468, 592, 882, 1158
BlsI	GCNGC	4 cut(s)	469, 593, 883, 1159
Bme1390I	CCNGG	1 cut(s)	809
Bme18I	GGWCC	2 cut(s)	128, 257
BmeT110I	CYCGRG	1 cut(s)	719
BmgBI	CACGTC	1 cut(s)	1177
BmgT120I	GGNCC	2 cut(s)	128, 257
BmiI	GGNNCC	1 cut(s)	717
BmrFI	CCNGG	1 cut(s)	809
BmsI	GCATC	3 cut(s)	207, 790, 1119
BsaHI	GRCGYC	1 cut(s)	1106
BsaJI	CCNNGG	2 cut(s)	71, 808
Bsc4I	CCNNNNNNNGG	2 cut(s)	266, 872
Bse1I	ACTGG	4 cut(s)	163, 259, 424, 1161
BseBI	CCWGG	1 cut(s)	809
BseDI	CCNNGG	2 cut(s)	71, 808
BseGI	GGATG	3 cut(s)	176, 410, 805
BseLI	CCNNNNNNNGG	2 cut(s)	266, 872
BseMII	CTCAG	2 cut(s)	225, 558
BseNI	ACTGG	4 cut(s)	163, 259, 424, 1161
BseRI	GAGGAG	2 cut(s)	88, 686
BseXI	GCAGC	3 cut(s)	479, 603, 868
Bsh1236I	CGCG	1 cut(s)	541
BsiHKAI	GWGCWC	1 cut(s)	402
BsiHKCI	CYCGRG	1 cut(s)	719
BsiSI	CCGG	1 cut(s)	521
BslFI	GGGAC	2 cut(s)	1029, 1117
BslI	CCNNNNNNNGG	2 cut(s)	266, 872
BsmAI	GTCTC	1 cut(s)	1043
BsmFI	GGGAC	2 cut(s)	1029, 1117
BsoBI	CYCGRG	1 cut(s)	719
Bsp1286I	GDGCHC	1 cut(s)	402
Bsp1407I	TGTACA	1 cut(s)	1171
Bsp143I	GATC	6 cut(s)	105, 371, 549, 953, 1008, 1180
BspACI	CCGC	2 cut(s)	13, 1158
BspCNI	CTCAG	2 cut(s)	226, 557
BspFNI	CGCG	1 cut(s)	541
BspLI	GGNNCC	1 cut(s)	717
BspMAI	CTGCAG	2 cut(s)	469, 886
BspPI	GGATC	5 cut(s)	379, 544, 961, 1016, 1175
BsrGI	TGTACA	1 cut(s)	1171
BsrI	ACTGG	4 cut(s)	163, 259, 424, 1161
BssECI	CCNNGG	2 cut(s)	71, 808
BssMI	GATC	6 cut(s)	105, 371, 549, 953, 1008, 1180
BssNI	GRCGYC	1 cut(s)	1106
BssSI	CACGAG	2 cut(s)	18, 473
Bst2BI	CACGAG	2 cut(s)	18, 473
Bst2UI	CCWGG	1 cut(s)	809
Bst4CI	ACNGT	4 cut(s)	265, 766, 1044, 1072
Bst6I	CTCTTC	1 cut(s)	705
BstACI	GRCGYC	1 cut(s)	1106
BstAPI	GCANNNNNTGC	1 cut(s)	890
BstAUI	TGTACA	1 cut(s)	1171
BstC8I	GCNNGC	4 cut(s)	619, 749, 753, 891
BstDEI	CTNAG	4 cut(s)	190, 234, 544, 638
BstF5I	GGATG	3 cut(s)	176, 410, 805
BstFNI	CGCG	1 cut(s)	541
BstHHI	GCGC	1 cut(s)	543
BstKTI	GATC	6 cut(s)	108, 374, 552, 956, 1011, 1183
BstMAI	GTCTC	1 cut(s)	1043
BstMBI	GATC	6 cut(s)	105, 371, 549, 953, 1008, 1180
BstMWI	GCNNNNNNNGC	4 cut(s)	29, 195, 397, 890
BstNI	CCWGG	1 cut(s)	809
BstNSI	RCATGY	3 cut(s)	391, 835, 893
BstSCI	CCNGG	1 cut(s)	807
BstSFI	CTRYAG	2 cut(s)	465, 882
BstUI	CGCG	1 cut(s)	541
BstV1I	GCAGC	3 cut(s)	479, 603, 868
BstX2I	RGATCY	1 cut(s)	549
BstYI	RGATCY	1 cut(s)	549
BsuI	GTATCC	1 cut(s)	782
BtrI	CACGTC	1 cut(s)	1177
BtsCI	GGATG	3 cut(s)	176, 410, 805
BtsIMutI	CAGTG	5 cut(s)	231, 252, 417, 856, 1077
Cac8I	GCNNGC	4 cut(s)	619, 749, 753, 891
CfoI	GCGC	1 cut(s)	543
Cfr13I	GGNCC	2 cut(s)	128, 257
CseI	GACGC	2 cut(s)	206, 804
Csp6I	GTAC	2 cut(s)	203, 1172
CviQI	GTAC	2 cut(s)	203, 1172
DdeI	CTNAG	4 cut(s)	190, 234, 544, 638
DpnI	GATC	6 cut(s)	107, 373, 551, 955, 1010, 1182
DpnII	GATC	6 cut(s)	105, 371, 549, 953, 1008, 1180
DraI	TTTAAA	2 cut(s)	97, 1120
Eam1104I	CTCTTC	1 cut(s)	705
EarI	CTCTTC	1 cut(s)	705
Eco32I	GATATC	1 cut(s)	931
Eco47I	GGWCC	2 cut(s)	128, 257
Eco57I	CTGAAG	2 cut(s)	169, 1170
Eco88I	CYCGRG	1 cut(s)	719
EcoRI	GAATTC	1 cut(s)	478
EcoRII	CCWGG	1 cut(s)	807
EcoRV	GATATC	1 cut(s)	931
EcoT22I	ATGCAT	1 cut(s)	328
FaqI	GGGAC	2 cut(s)	1029, 1117
FauNDI	CATATG	2 cut(s)	54, 613
Fnu4HI	GCNGC	4 cut(s)	468, 592, 882, 1158
FokI	GGATG	3 cut(s)	183, 417, 812
Fsp4HI	GCNGC	4 cut(s)	468, 592, 882, 1158
FspBI	CTAG	2 cut(s)	221, 605
GlaI	GCGC	1 cut(s)	542
GluI	GCNGC	4 cut(s)	468, 592, 882, 1158
HapII	CCGG	1 cut(s)	521
HgaI	GACGC	2 cut(s)	206, 804
HhaI	GCGC	1 cut(s)	543
Hin1I	GRCGYC	1 cut(s)	1106
Hin6I	GCGC	1 cut(s)	541
HinP1I	GCGC	1 cut(s)	541
HindIII	AAGCTT	1 cut(s)	923
HinfI	GANTC	5 cut(s)	4, 426, 681, 698, 821
HpaII	CCGG	1 cut(s)	521
HphI	GGTGA	1 cut(s)	701
Hpy166II	GTNNAC	1 cut(s)	1174
Hpy188I	TCNGA	8 cut(s)	72, 404, 547, 568, 703, 820, 941, 1189
Hpy188III	TCNNGA	7 cut(s)	18, 48, 475, 483, 582, 719, 786
Hpy8I	GTNNAC	1 cut(s)	1174
Hpy99I	CGWCG	1 cut(s)	1181
HpyAV	CCTTC	6 cut(s)	421, 671, 910, 997, 1094, 1194
HpyCH4III	ACNGT	4 cut(s)	265, 766, 1044, 1072
HpyCH4IV	ACGT	2 cut(s)	1106, 1176
HpyCH4V	TGCA	6 cut(s)	326, 467, 884, 893, 947, 1096
HpyF10VI	GCNNNNNNNGC	4 cut(s)	29, 195, 397, 890
HpyF3I	CTNAG	4 cut(s)	190, 234, 544, 638
HpySE526I	ACGT	2 cut(s)	1106, 1176
Hsp92I	GRCGYC	1 cut(s)	1106
HspAI	GCGC	1 cut(s)	541
Kzo9I	GATC	6 cut(s)	105, 371, 549, 953, 1008, 1180
LmnI	GCTCC	2 cut(s)	721, 812
Lsp1109I	GCAGC	3 cut(s)	479, 603, 868
LweI	GCATC	3 cut(s)	207, 790, 1119
MaeI	CTAG	2 cut(s)	221, 605
MaeII	ACGT	2 cut(s)	1106, 1176
MaeIII	GTNAC	1 cut(s)	15
MalI	GATC	6 cut(s)	107, 373, 551, 955, 1010, 1182
MboI	GATC	6 cut(s)	105, 371, 549, 953, 1008, 1180
MboII	GAAGA	5 cut(s)	172, 175, 406, 704, 722
MfeI	CAATTG	3 cut(s)	741, 867, 948
MflI	RGATCY	1 cut(s)	549
MhlI	GDGCHC	1 cut(s)	402
MluCI	AATT	7 cut(s)	444, 478, 741, 867, 948, 1033, 1198
MlyI	GAGTC	2 cut(s)	690, 815
MmeI	TCCRAC	1 cut(s)	408
MnlI	CCTC	6 cut(s)	66, 482, 664, 667, 700, 706
Mph1103I	ATGCAT	1 cut(s)	328
MseI	TTAA	7 cut(s)	96, 278, 329, 512, 761, 1119, 1201
MslI	CAYNNNNRTG	4 cut(s)	305, 836, 894, 977
MspA1I	CMGCKG	1 cut(s)	594
MspI	CCGG	1 cut(s)	521
MspR9I	CCNGG	1 cut(s)	809
MunI	CAATTG	3 cut(s)	741, 867, 948
MvaI	CCWGG	1 cut(s)	809
MvnI	CGCG	1 cut(s)	541
MwoI	GCNNNNNNNGC	4 cut(s)	29, 195, 397, 890
NdeI	CATATG	2 cut(s)	54, 613
NdeII	GATC	6 cut(s)	105, 371, 549, 953, 1008, 1180
NlaIV	GGNNCC	1 cut(s)	717
NmuCI	GTSAC	1 cut(s)	15
NsiI	ATGCAT	1 cut(s)	328
NspI	RCATGY	3 cut(s)	391, 835, 893
PaeI	GCATGC	1 cut(s)	893
PfeI	GAWTC	3 cut(s)	4, 426, 698
PflMI	CCANNNNNTGG	1 cut(s)	266
PkrI	GCNGC	4 cut(s)	469, 593, 883, 1159
PleI	GAGTC	2 cut(s)	689, 815
PpsI	GAGTC	2 cut(s)	689, 815
PsiI	TTATAA	1 cut(s)	660
Psp6I	CCWGG	1 cut(s)	807
PspGI	CCWGG	1 cut(s)	807
PspN4I	GGNNCC	1 cut(s)	717
PspPI	GGNCC	2 cut(s)	128, 257
PstI	CTGCAG	2 cut(s)	469, 886
PsuI	RGATCY	1 cut(s)	549
PvuII	CAGCTG	1 cut(s)	594
RsaI	GTAC	2 cut(s)	204, 1173
RsaNI	GTAC	2 cut(s)	203, 1172
RseI	CAYNNNNRTG	4 cut(s)	305, 836, 894, 977
SaqAI	TTAA	7 cut(s)	96, 278, 329, 512, 761, 1119, 1201
SatI	GCNGC	4 cut(s)	468, 592, 882, 1158
Sau3AI	GATC	6 cut(s)	105, 371, 549, 953, 1008, 1180
Sau96I	GGNCC	2 cut(s)	128, 257
SchI	GAGTC	2 cut(s)	690, 815
ScrFI	CCNGG	1 cut(s)	809
SduI	GDGCHC	1 cut(s)	402
SfaNI	GCATC	3 cut(s)	207, 790, 1119
SfcI	CTRYAG	2 cut(s)	465, 882
SinI	GGWCC	2 cut(s)	128, 257
SmiMI	CAYNNNNRTG	4 cut(s)	305, 836, 894, 977
SphI	GCATGC	1 cut(s)	893
Sse9I	AATT	7 cut(s)	444, 478, 741, 867, 948, 1033, 1198
SsiI	CCGC	2 cut(s)	13, 1158
SspMI	CTAG	2 cut(s)	221, 605
StyD4I	CCNGG	1 cut(s)	807
TaaI	ACNGT	4 cut(s)	265, 766, 1044, 1072
TaiI	ACGT	2 cut(s)	1109, 1179
TaqI	TCGA	1 cut(s)	1179
TasI	AATT	7 cut(s)	444, 478, 741, 867, 948, 1033, 1198
TatI	WGTACW	1 cut(s)	1171
TauI	GCSGC	1 cut(s)	1160
TfiI	GAWTC	3 cut(s)	4, 426, 698
Tru1I	TTAA	7 cut(s)	96, 278, 329, 512, 761, 1119, 1201
Tru9I	TTAA	7 cut(s)	96, 278, 329, 512, 761, 1119, 1201
TscAI	CASTG	5 cut(s)	238, 259, 424, 856, 1077
TseFI	GTSAC	1 cut(s)	15
TseI	GCWGC	3 cut(s)	467, 591, 881
Tsp45I	GTSAC	1 cut(s)	15
TspDTI	ATGAA	4 cut(s)	422, 521, 711, 929
TspGWI	ACGGA	2 cut(s)	351, 766
TspRI	CASTG	5 cut(s)	238, 259, 424, 856, 1077
Van91I	CCANNNNNTGG	1 cut(s)	266
VpaK11BI	GGWCC	2 cut(s)	128, 257
XapI	RAATTY	3 cut(s)	444, 478, 1033
XceI	RCATGY	3 cut(s)	391, 835, 893
XspI	CTAG	2 cut(s)	221, 605
ZraI	GACGTC	1 cut(s)	1107
Zsp2I	ATGCAT	1 cut(s)	328

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.