Rorug02G0421000

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003474
Physical Location & Seq
Reverse (-)
54083805 .. 54094067
10263 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0421000.1

Sequence Viewer

Length: 3675 bp
ATGTACGGCGGCTCATCCAAGCACGGCCGATTAGGCGGCGGCGCCGGCCGGGGAGCCGGAGCCGCCAAGCTCGGCCGAGCCTCCTTCCCTCCGCCTACCCGCAACTCCGCCGCCCCAGCAGCAGCCAACCGCCTCTCCCTCGGCGGCTCCAACCATCGAGGAGGCCGTAACCCCCCCGCCCCTGCCACGTCAGCCGCCGCCCCGGCCGTGGAGGAGAAGTTCAGCTTAGTCGCCGGCAACAATCCGGTGGCGTTCTCCATGATCATTCGGTTGGTGCCGGACTTGGTCGACGAGATCAGGCGCGTCGAGGCCCAAGGTGGGACCCCCCGCATCAAATTTGACTCCATGACTAATAATCCCAACGGCAATGTTATTGATGTGGGTGGGAAGGAGTTTAGATTTACATGGTCTCGGGATGGTGACCTCTGTGACATATATGAAGAACGTGAAACTGGTGAAGATGGAAATGGTTTGCTTGTTGAATCAGGATGTGCATGGCGCAAAGTGAATGTTCAGCGTATATTAGATGAGTCAACTAAGAATCATGTTAAGAGGCGGTCGGAGGAAGCTGAACGCTTGCACAAATCACGCAAAGCCATTGTGCTAGAACAGAATCCAGCCATGAAGAATCAACTAAAGCAATTTACTGCTGCCACGGCTAATAGTTCATGGAGGAATTTCAAACAGAAGAAAGAGCCTCCCTTTAAAAAGCAGAAAGTTGAACCACCTCAAGCTCCTCCAAAGTCTGCATATAAATCTGGGGTATCATCAGCAACTACTGCAAAGGGTAGACAGTCATCATCACCTCTTCCGTCTACACCTGAGAAGTCTGCATCTCCATTTGGAATGCTAAATATTTCAAAGACTCATACAAGTGCAGATGAAACTATACCCTCTCAAGTCATTATTAAGGACAAACCTGCTCCTAGCTTGGATAAAGAAACCTCAATCAGGACTAGTGCAGCATGGGAAACTGCTGGCAAAGGCAGTGGTCGAGCTAAGCCAACAGATTTGCAGAGTTTGTTGATTACACTGCTCATGGAGAATCCCAAGGGGATGAGCTTAAAGGCTTTGGAGAAAGCTATTGGAGATTCATTTCCTAACTCTGTGAAAAAAATTGAGCCCATTCTTAAGAAGATCGCAAATTCCCAAGCTCCAGGGAAGTATCTCTTGAAGCCAGGAGTGGAGTTAGAAAGCTTCAAGAAACCTTTGTTTGAATCTGGAAGTTCCCCAGAAGAGAACCTTCGCCGGACTCCTGCTACGGATAACGTCTGTGATCCTTTGCCTGCTCAAATACCAGAGTTTAAGGAGAAGGTTTCTTCCAGCGAAATGGAGGAACAGGGCCAGGGACAATTGAATGCTACAGTTGGAGAAGAACCACAGGTATTCCAAAAAATTGATAACCAACAGCAGTCACCTGATCTTTTTGGTGAAAAAAGGGCCTCTGACAACAGTGAAGCCCAAATAGGTAGTTCTAGTTCTAGTGACAGTGGAAGTGACAGTGACAGTGAAAGTGACAGCAGTGATTCCGGAAGTGACAGTGGAAGTCCTAGCAGAAGTAAAAGCCCAAAAGGAAGTGGGAGTGGGAGTAGCAGCGATAGTGAAAGTGATGTGTCTTCTAATAGCAAGGAGGTTTCTGATGAGGATGTGAACATTATGAGTGATGATGATAAAGAGCCCAAACATCAAACACAAGCCTCTGAAGCTGCTTTGCCCATTCCATGGGAAACTCCTGATGGCAGGCCTGTGCAGAGTGGGAATGATGACAAGCAAGATGATCTTGAATCTGATGCAATTGATATTGAAACTGTGGAACAGGATGCTGAATTGGCTGTTGTTAGTAGCTCCGCTCCTAATAAATGTGATCAACCTGTTGATGAAACAAAACCTTCTTCACCTGAGCATGTTGAGCATCATGACCGCCAGATTCTTGTAGACAGTTTGTTTGGAAAGACTGCAGACAAGGATGACTTCAGGTATGAGCAGTCTGATAGCTCTGAGAGGATGACTAAAGGTAAATCCAAAAGAGGTTTGGAGGTAAAGCACTCTGATGAAAAATCTGAACGCAGAAAAAGGTCAAAAACGGAAAACTCACCACAACCTCCTGCTTCTGTGGGTAAGGGTGTTCAATTTTCAGAGGATTATCATAATTTGTCTCCTGATAGACTCATTGAAGGCTCTTATAAGGGCCCTGTATCTCAAACGCTAAATAAAGCTGATAGGGATGGGAATACTGAGTTTGGCTCTCAAAAGGGCTACAATCAAGCAGTTTCTGGAAGATATAGCTCGGAGTCTAAACAATTAAGTCGGAAGTCTTATGATCATAGTGCACAAGCAAAGGCTCCTGATCAAGGTGAAAGACCTGATAGATATTCCGAGAGCTTGGGCCATGGTCTGCAGTACTCTGAAAAGAGCTCTCATGTGCATGAAGGCTTTCCCCTGCGAAAAGATAATGTTCATAGTAGTAGTCAAAAGGACGGTTATGCTAATGAAAAGAAGGTTTTAAGAAATTCCAAGGAAGGTGGTGGCCGAGGCAAACAATCAGTGCCCTTGGACTCCCACTACCAGAAACATGGTGATGTGGCTGGCAAAATCAAGGAAGATAGACAGGTTTCAAGTATGTTCTTCAGTTCTTCGCCGAAGGAAAACAGGAATGGAGCCGACAGATCCCCAGTTGTTAATGGTAGAGGTAGTAAACTCCAAAGAGAGGTTTCAGAATTGGAGTTGGGCGAACTTCGTGAGGCCGTGCCCGAGGAAACAACAGTTAAGAAGCAATTTGAGAGAAAAAGTTCATTTAAGCAGTCAGAAAACAAACTGGGCACCAGGGATACCTGGGCTTCAGACTTCAGTAAATCAAAACCTGCAGGAAAGCCATCCTCACCAGATTTAAACTTGAAGTTTCCTAGTAATGCAGAAGGCTTAGTTAAAAAAAGGAACTACGAAGATCGTATTGAGGATTTAACGGGGTCTCAACAGAGAGCTCTGCAGTCTCAGTCACTGCACCTTTCACGAGTAGATAATTCTGATGTGGGGCGCCCATTCAGCAGATCAGCAGATAACAGTAGTAAATCAATACAAAATGAAGTTGGGGGCAGACAAGGGATCAGTTTGGATGGCTATGGAGAAAGCAATAAGAAAGCACCTCCTAGGGCTTCTCAGCACCATGACTCTAAACGAGGGCCACTGTCCCACACCAATAAAGAAAGTAAAAGACTTGCATCTAGTATGATGATGGAACAGACTGATGTACGAAAGGATTCAATGTTGGCAGAGGGCACCGACAGTGATAGAAAAAGAAGGGATTCTTCTTCTGATGAAAATAGTTGTTCTTATACAAAGTATGAGAAGGATGAGCCTGAGCTCAAGGGACCAATAAAAGATTTCTCTCAGTACAAGGAGTATGTGCAAGAATATCATGATAAGTATGATAGCTACTGCTCGTTAAACAAGATCTTAGAGAGTTACAGGAATGAGTTCCTAAAGCTAGGGAAGGACCTTGAATTTGCTTCGGGCACAGAGAGATATTATAACATCTTGGACCAGCTGAAGGAGTCCTATCGTCAGTGTGGATCGAGACATAAGAGGCTCAAAAAGATATTTGTGGTGCTTCATGAGGAACTAAAGCACCTTAAGCAAAGGATCAAAGAGTTTGCACTCCCATACACGAAGGACTGA

Protein Analysis

1224

Amino Acids

134.7

Weight (kDa)

7.86

Isoelectric Point (pI)

56.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Occludin_ELL PF07303 1123 - 1218 6.9e-19 Occludin homology domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2184, 3528
Acc36I ACCTGC 2 cut(s) 928, 2869
AccB1I GGYRCC 5 cut(s) 41, 274, 2819, 3033, 3275
AccB7I CCANNNNNTGG 1 cut(s) 1722
AccBSI CCGCTC 1 cut(s) 1850
AccI GTMKAC 4 cut(s) 288, 790, 815, 1935
AccII CGCG 1 cut(s) 303
AccIII TCCGGA 1 cut(s) 1529
AclWI GGATC 5 cut(s) 1271, 2661, 3110, 3577, 3647
AcoI YGGCCR 5 cut(s) 25, 46, 73, 204, 2527
AcsI RAATTY 5 cut(s) 335, 676, 1144, 2509, 3500
AcuI CTGAAG 6 cut(s) 1722, 1957, 2611, 2823, 2830, 3566
AcyI GRCGYC 2 cut(s) 42, 3034
AfaI GTAC 4 cut(s) 5, 2402, 3249, 3392
AfiI CCNNNNNNNGG 7 cut(s) 208, 318, 951, 1722, 2351, 2707, 3547
AflII CTTAAG 2 cut(s) 1130, 3629
AhlI ACTAGT 1 cut(s) 956
AjiI CACGTC 1 cut(s) 189
AjnI CCWGG 5 cut(s) 1156, 1177, 1344, 2822, 2831
AloI GAACNNNNNNTCC 2 cut(s) 203, 235
Alw21I GWGCWC 4 cut(s) 2332, 2417, 2983, 3363
Alw26I GTCTC 5 cut(s) 414, 2160, 2973, 2994, 3568
Alw44I GTGCAC 1 cut(s) 2328
AlwI GGATC 5 cut(s) 1271, 2661, 3110, 3577, 3647
AlwNI CAGNNNCTG 2 cut(s) 2273, 2998
Ama87I CYCGRG 2 cut(s) 411, 2750
Aor13HI TCCGGA 1 cut(s) 1529
ApaI GGGCCC 1 cut(s) 2192
ApaLI GTGCAC 1 cut(s) 2328
ApeKI GCWGC 6 cut(s) 119, 122, 650, 962, 1593, 1706
ApoI RAATTY 5 cut(s) 335, 676, 1144, 2509, 3500
ArsI GACNNNNNNTTYG 4 cut(s) 1928, 1960, 3485, 3517
Asp700I GAANNNNTTC 5 cut(s) 2433, 2788, 3256, 3301, 3473
AspA2I CCTAGG 1 cut(s) 3146
AspLEI GCGC 4 cut(s) 44, 303, 501, 3036
AsuC2I CCSGG 2 cut(s) 50, 203
AvaI CYCGRG 2 cut(s) 411, 2750
AvaII GGWCC 4 cut(s) 321, 3368, 3493, 3538
AvrII CCTAGG 1 cut(s) 3146
BaeGI GKGCMC 7 cut(s) 2192, 2332, 2550, 2751, 2822, 3278, 3515
BanI GGYRCC 5 cut(s) 41, 274, 2819, 3033, 3275
BanII GRGCYC 6 cut(s) 1125, 1680, 2192, 2417, 2983, 3363
BauI CACGAG 1 cut(s) 3009
BbsI GAAGAC 1 cut(s) 1608
Bbv12I GWGCWC 4 cut(s) 2332, 2417, 2983, 3363
BbvI GCAGC 6 cut(s) 131, 134, 637, 974, 1605, 1693
BccI CCATC 8 cut(s) 162, 410, 455, 1730, 2219, 2881, 3107, 3226
BceAI ACGGC 7 cut(s) 22, 40, 150, 191, 379, 672, 2729
BciT130I CCWGG 5 cut(s) 1158, 1179, 1346, 2824, 2833
BciVI GTATCC 1 cut(s) 2821
BclI TGATCA 4 cut(s) 261, 1864, 2320, 2347
BcnI CCSGG 2 cut(s) 50, 203
BcoDI GTCTC 5 cut(s) 414, 2160, 2973, 2994, 3568
BcuI ACTAGT 1 cut(s) 956
BfmI CTRYAG 5 cut(s) 1362, 1956, 2396, 2862, 2984
BfoI RGCGCY 2 cut(s) 45, 3037
BfrI CTTAAG 2 cut(s) 1130, 3629
BfuAI ACCTGC 2 cut(s) 928, 2869
BfuI GTATCC 1 cut(s) 2821
BglI GCCNNNNNGGC 2 cut(s) 33, 203
BglII AGATCT 1 cut(s) 3450
BlnI CCTAGG 1 cut(s) 3146
BlpI GCTNAGC 1 cut(s) 999
BmcAI AGTACT 1 cut(s) 2402
Bme1390I CCNGG 7 cut(s) 50, 203, 1158, 1179, 1346, 2824, 2833
Bme18I GGWCC 4 cut(s) 321, 3368, 3493, 3538
BmeT110I CYCGRG 2 cut(s) 411, 2750
BmgBI CACGTC 1 cut(s) 189
BmrFI CCNGG 7 cut(s) 50, 203, 1158, 1179, 1346, 2824, 2833
BmrI ACTGGG 2 cut(s) 2666, 2825
BmsI GCATC 6 cut(s) 339, 842, 1780, 1810, 1921, 3227
BmuI ACTGGG 2 cut(s) 2666, 2825
BpiI GAAGAC 1 cut(s) 1608
BplI GAGNNNNNCTC 2 cut(s) 2228, 2260
BpmI CTGGAG 1 cut(s) 1140
Bpu10I CCTNAGC 2 cut(s) 1899, 3357
Bpu1102I GCTNAGC 1 cut(s) 999
BpuEI CTTGAG 3 cut(s) 714, 882, 3347
BpuMI CCSGG 2 cut(s) 50, 203
BsaHI GRCGYC 2 cut(s) 42, 3034
BsaI GGTCTC 2 cut(s) 414, 2973
BsaWI WCCGGW 2 cut(s) 244, 1529
BsaXI ACNNNNNCTCC 6 cut(s) 119, 149, 203, 233, 1573, 1603
Bsc4I CCNNNNNNNGG 7 cut(s) 208, 318, 951, 1722, 2351, 2707, 3547
Bse118I RCCGGY 2 cut(s) 44, 233
Bse1I ACTGG 3 cut(s) 457, 2672, 2820
Bse3DI GCAATG 1 cut(s) 373
BseAI TCCGGA 1 cut(s) 1529
BseBI CCWGG 5 cut(s) 1158, 1179, 1346, 2824, 2833
BseLI CCNNNNNNNGG 7 cut(s) 208, 318, 951, 1722, 2351, 2707, 3547
BseMI GCAATG 1 cut(s) 373
BseMII CTCAG 8 cut(s) 813, 1890, 1989, 2226, 3005, 3170, 3348, 3401
BseNI ACTGG 3 cut(s) 457, 2672, 2820
BseRI GAGGAG 3 cut(s) 174, 227, 726
BseSI GKGCMC 7 cut(s) 2192, 2332, 2550, 2751, 2822, 3278, 3515
BseX3I CGGCCG 4 cut(s) 25, 46, 73, 204
BseXI GCAGC 6 cut(s) 131, 134, 637, 974, 1605, 1693
BseYI CCCAGC 1 cut(s) 115
BsgI GTGCAG 4 cut(s) 897, 981, 1769, 2984
Bsh1236I CGCG 1 cut(s) 303
Bsh1285I CGRYCG 5 cut(s) 28, 49, 76, 207, 560
BshNI GGYRCC 5 cut(s) 41, 274, 2819, 3033, 3275
BsiEI CGRYCG 5 cut(s) 28, 49, 76, 207, 560
BsiHKAI GWGCWC 4 cut(s) 2332, 2417, 2983, 3363
BsiHKCI CYCGRG 2 cut(s) 411, 2750
BsiSI CCGG 9 cut(s) 45, 49, 57, 203, 234, 245, 278, 1249, 1530
BslFI GGGAC 4 cut(s) 334, 1362, 3172, 3381
BslI CCNNNNNNNGG 7 cut(s) 208, 318, 951, 1722, 2351, 2707, 3547
BsmAI GTCTC 5 cut(s) 414, 2160, 2973, 2994, 3568
BsmFI GGGAC 4 cut(s) 334, 1362, 3172, 3381
BsmI GAATGC 2 cut(s) 852, 1363
Bso31I GGTCTC 2 cut(s) 414, 2973
BsoBI CYCGRG 2 cut(s) 411, 2750
Bsp120I GGGCCC 1 cut(s) 2188
Bsp13I TCCGGA 1 cut(s) 1529
Bsp1720I GCTNAGC 1 cut(s) 999
Bsp19I CCATGG 2 cut(s) 1721, 2389
BspCNI CTCAG 8 cut(s) 814, 1891, 1990, 2227, 3004, 3169, 3349, 3400
BspEI TCCGGA 1 cut(s) 1529
BspFNI CGCG 1 cut(s) 303
BspHI TCATGA 3 cut(s) 1915, 3415, 3610
BspMAI CTGCAG 4 cut(s) 1960, 2400, 2866, 2988
BspMI ACCTGC 2 cut(s) 928, 2869
BspPI GGATC 5 cut(s) 1271, 2661, 3110, 3577, 3647
BspT107I GGYRCC 5 cut(s) 41, 274, 2819, 3033, 3275
BspTI CTTAAG 2 cut(s) 1130, 3629
BspTNI GGTCTC 2 cut(s) 414, 2973
BsrBI CCGCTC 1 cut(s) 1850
BsrDI GCAATG 1 cut(s) 373
BsrFI RCCGGY 2 cut(s) 44, 233
BsrI ACTGG 3 cut(s) 457, 2672, 2820
BssAI RCCGGY 2 cut(s) 44, 233
BssNI GRCGYC 2 cut(s) 42, 3034
BssSI CACGAG 1 cut(s) 3009
BssT1I CCWWGG 7 cut(s) 313, 1050, 1721, 2389, 2514, 2550, 3146
Bst2BI CACGAG 1 cut(s) 3009
Bst2UI CCWGG 5 cut(s) 1158, 1179, 1346, 2824, 2833
Bst6I CTCTTC 2 cut(s) 813, 1230
BstACI GRCGYC 2 cut(s) 42, 3034
BstAFI CTTAAG 2 cut(s) 1130, 3629
BstAPI GCANNNNNTGC 1 cut(s) 779
BstC8I GCNNGC 7 cut(s) 46, 235, 578, 979, 1287, 1742, 2587
BstDSI CCRYGG 4 cut(s) 207, 654, 1721, 2389
BstEII GGTNACC 1 cut(s) 419
BstENI CCTNNNNNAGG 2 cut(s) 949, 2349
BstFNI CGCG 1 cut(s) 303
BstH2I RGCGCY 2 cut(s) 45, 3037
BstHHI GCGC 4 cut(s) 44, 303, 501, 3036
BstMAI GTCTC 5 cut(s) 414, 2160, 2973, 2994, 3568
BstMCI CGRYCG 5 cut(s) 28, 49, 76, 207, 560
BstNI CCWGG 5 cut(s) 1158, 1179, 1346, 2824, 2833
BstNSI RCATGY 1 cut(s) 1907
BstPI GGTNACC 1 cut(s) 419
BstSCI CCNGG 7 cut(s) 48, 201, 1156, 1177, 1344, 2822, 2831
BstSFI CTRYAG 5 cut(s) 1362, 1956, 2396, 2862, 2984
BstSLI GKGCMC 7 cut(s) 2192, 2332, 2550, 2751, 2822, 3278, 3515
BstUI CGCG 1 cut(s) 303
BstV1I GCAGC 6 cut(s) 131, 134, 637, 974, 1605, 1693
BstV2I GAAGAC 1 cut(s) 1608
BstX2I RGATCY 2 cut(s) 2666, 3450
BstXI CCANNNNNNTGG 2 cut(s) 1330, 2573
BstYI RGATCY 2 cut(s) 2666, 3450
BstZI CGGCCG 4 cut(s) 25, 46, 73, 204
BsuI GTATCC 1 cut(s) 2821
BtgI CCRYGG 4 cut(s) 207, 654, 1721, 2389
BtrI CACGTC 1 cut(s) 189
BtsI GCAGTG 4 cut(s) 994, 1031, 1528, 2996
BveI ACCTGC 2 cut(s) 928, 2869
Cac8I GCNNGC 7 cut(s) 46, 235, 578, 979, 1287, 1742, 2587
CaiI CAGNNNCTG 2 cut(s) 2273, 2998
CciI TCATGA 3 cut(s) 1915, 3415, 3610
CfoI GCGC 4 cut(s) 44, 303, 501, 3036
Cfr10I RCCGGY 2 cut(s) 44, 233
CseI GACGC 1 cut(s) 292
Csp6I GTAC 4 cut(s) 4, 2401, 3248, 3391
CspCI CAANNNNNGTGG 4 cut(s) 970, 1005, 2503, 2538
CviQI GTAC 4 cut(s) 4, 2401, 3248, 3391
DinI GGCGCC 2 cut(s) 43, 3035
DraI TTTAAA 2 cut(s) 706, 2889
EaeI YGGCCR 5 cut(s) 25, 46, 73, 204, 2527
EagI CGGCCG 4 cut(s) 25, 46, 73, 204
Eam1104I CTCTTC 2 cut(s) 813, 1230
EarI CTCTTC 2 cut(s) 813, 1230
EciI GGCGGA 2 cut(s) 81, 97
Ecl136II GAGCTC 3 cut(s) 2415, 2981, 3361
EclXI CGGCCG 4 cut(s) 25, 46, 73, 204
Eco130I CCWWGG 7 cut(s) 313, 1050, 1721, 2389, 2514, 2550, 3146
Eco147I AGGCCT 1 cut(s) 1744
Eco24I GRGCYC 6 cut(s) 1125, 1680, 2192, 2417, 2983, 3363
Eco31I GGTCTC 2 cut(s) 414, 2973
Eco47I GGWCC 4 cut(s) 321, 3368, 3493, 3538
Eco52I CGGCCG 4 cut(s) 25, 46, 73, 204
Eco53kI GAGCTC 3 cut(s) 2415, 2981, 3361
Eco57I CTGAAG 6 cut(s) 1722, 1957, 2611, 2823, 2830, 3566
Eco88I CYCGRG 2 cut(s) 411, 2750
Eco91I GGTNACC 1 cut(s) 419
EcoICRI GAGCTC 3 cut(s) 2415, 2981, 3361
EcoNI CCTNNNNNAGG 2 cut(s) 949, 2349
EcoO109I RGGNCCY 5 cut(s) 321, 1440, 2188, 2189, 3493
EcoO65I GGTNACC 1 cut(s) 419
EcoRII CCWGG 5 cut(s) 1156, 1177, 1344, 2822, 2831
EcoT14I CCWWGG 7 cut(s) 313, 1050, 1721, 2389, 2514, 2550, 3146
EcoT38I GRGCYC 6 cut(s) 1125, 1680, 2192, 2417, 2983, 3363
EgeI GGCGCC 2 cut(s) 43, 3035
EheI GGCGCC 2 cut(s) 43, 3035
ErhI CCWWGG 7 cut(s) 313, 1050, 1721, 2389, 2514, 2550, 3146
FaqI GGGAC 4 cut(s) 334, 1362, 3172, 3381
FauI CCCGC 3 cut(s) 107, 184, 335
FbaI TGATCA 4 cut(s) 261, 1864, 2320, 2347
FblI GTMKAC 4 cut(s) 288, 790, 815, 1935
FriOI GRGCYC 6 cut(s) 1125, 1680, 2192, 2417, 2983, 3363
GlaI GCGC 4 cut(s) 43, 302, 500, 3035
GsaI CCCAGC 1 cut(s) 119
GsuI CTGGAG 1 cut(s) 1140
HaeII RGCGCY 2 cut(s) 45, 3037
HapII CCGG 9 cut(s) 45, 49, 57, 203, 234, 245, 278, 1249, 1530
HgaI GACGC 1 cut(s) 292
HhaI GCGC 4 cut(s) 44, 303, 501, 3036
Hin1I GRCGYC 2 cut(s) 42, 3034
Hin6I GCGC 4 cut(s) 42, 301, 499, 3034
HinP1I GCGC 4 cut(s) 42, 301, 499, 3034
HincII GTYRAC 2 cut(s) 289, 534
HindII GTYRAC 2 cut(s) 289, 534
HindIII AAGCTT 1 cut(s) 1195
HpaII CCGG 9 cut(s) 45, 49, 57, 203, 234, 245, 278, 1249, 1530
Hpy166II GTNNAC 8 cut(s) 289, 534, 791, 816, 1651, 1936, 2330, 2696
Hpy8I GTNNAC 8 cut(s) 289, 534, 791, 816, 1651, 1936, 2330, 2696
Hpy99I CGWCG 2 cut(s) 293, 308
HpyCH4IV ACGT 3 cut(s) 188, 445, 1269
HpySE526I ACGT 3 cut(s) 188, 445, 1269
Hsp92I GRCGYC 2 cut(s) 42, 3034
HspAI GCGC 4 cut(s) 42, 301, 499, 3034
KasI GGCGCC 2 cut(s) 41, 3033
KflI GGGWCCC 1 cut(s) 321
Kpn2I TCCGGA 1 cut(s) 1529
KroI GCCGGC 2 cut(s) 44, 233
KroNI GCCGGC 2 cut(s) 46, 235
Ksp22I TGATCA 4 cut(s) 261, 1864, 2320, 2347
Lsp1109I GCAGC 6 cut(s) 131, 134, 637, 974, 1605, 1693
LweI GCATC 6 cut(s) 339, 842, 1780, 1810, 1921, 3227
MaeII ACGT 3 cut(s) 188, 445, 1269
MbiI CCGCTC 1 cut(s) 1850
MfeI CAATTG 2 cut(s) 1352, 1794
MflI RGATCY 2 cut(s) 2666, 3450
Mly113I GGCGCC 2 cut(s) 42, 3034
MlyI GAGTC 9 cut(s) 335, 539, 859, 1246, 2160, 2300, 2549, 3161, 3560
MmeI TCCRAC 4 cut(s) 174, 540, 1348, 2288
MroI TCCGGA 1 cut(s) 1529
MroNI GCCGGC 2 cut(s) 44, 233
MroXI GAANNNNTTC 5 cut(s) 2433, 2788, 3256, 3301, 3473
MslI CAYNNNNRTG 4 cut(s) 873, 2049, 2424, 2577
MspA1I CMGCKG 1 cut(s) 3544
MspCI CTTAAG 2 cut(s) 1130, 3629
MspI CCGG 9 cut(s) 45, 49, 57, 203, 234, 245, 278, 1249, 1530
MspR9I CCNGG 7 cut(s) 50, 203, 1158, 1179, 1346, 2824, 2833
MunI CAATTG 2 cut(s) 1352, 1794
Mva1269I GAATGC 2 cut(s) 852, 1363
MvaI CCWGG 5 cut(s) 1158, 1179, 1346, 2824, 2833
MvnI CGCG 1 cut(s) 303
NaeI GCCGGC 2 cut(s) 46, 235
NarI GGCGCC 2 cut(s) 42, 3034
NciI CCSGG 2 cut(s) 50, 203
NcoI CCATGG 2 cut(s) 1721, 2389
NgoMIV GCCGGC 2 cut(s) 44, 233
NmeAIII GCCGAG 4 cut(s) 51, 101, 120, 2555
NmuCI GTSAC 9 cut(s) 419, 428, 1413, 1484, 1496, 1502, 1514, 1535, 2994
NspI RCATGY 1 cut(s) 1907
PagI TCATGA 3 cut(s) 1915, 3415, 3610
PceI AGGCCT 1 cut(s) 1744
PcsI WCGNNNNNNNCGW 1 cut(s) 163
PctI GAATGC 2 cut(s) 852, 1363
PdiI GCCGGC 2 cut(s) 46, 235
PdmI GAANNNNTTC 5 cut(s) 2433, 2788, 3256, 3301, 3473
PflFI GACNNNGTC 1 cut(s) 284
PflMI CCANNNNNTGG 1 cut(s) 1722
PleI GAGTC 9 cut(s) 335, 538, 859, 1246, 2160, 2299, 2549, 3161, 3559
PluTI GGCGCC 2 cut(s) 45, 3037
PpsI GAGTC 9 cut(s) 335, 538, 859, 1246, 2160, 2299, 2549, 3161, 3559
PpuMI RGGWCCY 2 cut(s) 321, 3493
PsiI TTATAA 2 cut(s) 2184, 3528
Psp124BI GAGCTC 3 cut(s) 2417, 2983, 3363
Psp5II RGGWCCY 2 cut(s) 321, 3493
Psp6I CCWGG 5 cut(s) 1156, 1177, 1344, 2822, 2831
PspEI GGTNACC 1 cut(s) 419
PspFI CCCAGC 1 cut(s) 115
PspGI CCWGG 5 cut(s) 1156, 1177, 1344, 2822, 2831
PspOMI GGGCCC 1 cut(s) 2188
PspPPI RGGWCCY 2 cut(s) 321, 3493
PsrI GAACNNNNNNTAC 2 cut(s) 1462, 1494
PstI CTGCAG 4 cut(s) 1960, 2400, 2866, 2988
PstNI CAGNNNCTG 2 cut(s) 2273, 2998
PsuI RGATCY 2 cut(s) 2666, 3450
PsyI GACNNNGTC 1 cut(s) 284
PvuII CAGCTG 1 cut(s) 3544
RsaI GTAC 4 cut(s) 5, 2402, 3249, 3392
RsaNI GTAC 4 cut(s) 4, 2401, 3248, 3391
RseI CAYNNNNRTG 4 cut(s) 873, 2049, 2424, 2577
SacI GAGCTC 3 cut(s) 2417, 2983, 3363
SalI GTCGAC 1 cut(s) 287
SbfI CCTGCAGG 1 cut(s) 2866
ScaI AGTACT 1 cut(s) 2402
SchI GAGTC 9 cut(s) 335, 539, 859, 1246, 2160, 2300, 2549, 3161, 3560
ScrFI CCNGG 7 cut(s) 50, 203, 1158, 1179, 1346, 2824, 2833
SdaI CCTGCAGG 1 cut(s) 2866
SfaNI GCATC 6 cut(s) 339, 842, 1780, 1810, 1921, 3227
SfcI CTRYAG 5 cut(s) 1362, 1956, 2396, 2862, 2984
SfoI GGCGCC 2 cut(s) 43, 3035
SinI GGWCC 4 cut(s) 321, 3368, 3493, 3538
SmiMI CAYNNNNRTG 4 cut(s) 873, 2049, 2424, 2577
SmlI CTYRAG 5 cut(s) 729, 897, 1130, 3362, 3629
SmoI CTYRAG 5 cut(s) 729, 897, 1130, 3362, 3629
SpeI ACTAGT 1 cut(s) 956
Sse8387I CCTGCAGG 1 cut(s) 2866
SseBI AGGCCT 1 cut(s) 1744
SspDI GGCGCC 2 cut(s) 41, 3033
SspI AATATT 1 cut(s) 856
SstI GAGCTC 3 cut(s) 2417, 2983, 3363
StuI AGGCCT 1 cut(s) 1744
StyD4I CCNGG 7 cut(s) 48, 201, 1156, 1177, 1344, 2822, 2831
StyI CCWWGG 7 cut(s) 313, 1050, 1721, 2389, 2514, 2550, 3146
TaiI ACGT 3 cut(s) 191, 448, 1272
TaqI TCGA 5 cut(s) 157, 288, 306, 994, 3572
TatI WGTACW 2 cut(s) 2400, 3390
TauI GCSGC 8 cut(s) 12, 39, 42, 65, 113, 147, 197, 200
TseFI GTSAC 9 cut(s) 419, 428, 1413, 1484, 1496, 1502, 1514, 1535, 2994
TseI GCWGC 6 cut(s) 119, 122, 650, 962, 1593, 1706
Tsp45I GTSAC 9 cut(s) 419, 428, 1413, 1484, 1496, 1502, 1514, 1535, 2994
TspGWI ACGGA 3 cut(s) 801, 1277, 2099
Tth111I GACNNNGTC 1 cut(s) 284
Van91I CCANNNNNTGG 1 cut(s) 1722
Vha464I CTTAAG 2 cut(s) 1130, 3629
VneI GTGCAC 1 cut(s) 2328
VpaK11BI GGWCC 4 cut(s) 321, 3368, 3493, 3538
XagI CCTNNNNNAGG 2 cut(s) 949, 2349
XapI RAATTY 5 cut(s) 335, 676, 1144, 2509, 3500
XceI RCATGY 1 cut(s) 1907
XcmI CCANNNNNNNNNTGG 1 cut(s) 2029
XmaJI CCTAGG 1 cut(s) 3146
XmiI GTMKAC 4 cut(s) 288, 790, 815, 1935
XmnI GAANNNNTTC 5 cut(s) 2433, 2788, 3256, 3301, 3473
ZrmI AGTACT 1 cut(s) 2402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.