Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0004936

Physical Location & Seq

Genomic Coordinates Forward (+)

15210145 .. 15212604

Gene Structure 2460 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1177400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 657 bp

ATGGTGAAGCTGACAATGATTGCTCGTGTTACTGATGGTCTCCCACTAGCAGAGGGACTGGATGATGGCCGTGATGTTAAAGACTCGGAATTCTACAAACAACAAGTCAAGGCTTTATTCAAGAACTTGTCAACCGGACAAAATGAGCCTTCAAGGATGTCAGTTGAAACTGGACCTTTTGTTTTCCACTATATCATCGAAGGACGTGTGTGTTACCTGACAATGTGTGACCGTGCCTATCCAAAGAAACTTGCCTTCCAATACCTTGAAGATCTCAAGAATGAGTTTGAGCGTGTTAATGGGACCCAAATTGAAACTGCTGCAAGACCTTATGCATTCATTAAATTTGATACATTCATACAGAAAACAAAGAAATTGTACCAGGACACTCACACTCAGCGGAATATTGCAAAGTTGAATGATGAGCTGTATGAAGTCCACCAAATAATGACTCGCAATGTTCAGGAAGTGCTTGGTGTTGGTGAAAAGTTGGACCAGGTCAGTCAAATGTCGAGCCGTTTAACATCAGAATCTCGCATATATGCTGATAAAGCAAGAGATTTGAATCGACAGGCTCTGATCCGAAAGTACGCGCCTATTGCCATTGTGCTTGGAGTTGTTTTCCTCCTTTTCTGGGTCAAAACAAAGCTCTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000149 GO:0003674 GO:0005484 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0005886 GO:0006810 GO:0006887 GO:0006906 GO:0006996 GO:0008150 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0031201 GO:0032940 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0046903 GO:0048284 GO:0051179 GO:0051234 GO:0061024 GO:0061025 GO:0071840 GO:0071944 GO:0090174 GO:0098796

KEGG Pathways

Metabolic & Signaling

ko04130 ko04145 ko05134 map04130 map04145 map05134

Pfam Domains

Protein Families

Longin Synaptobrevin

Protein Analysis

219

Amino Acids

25.3

Weight (kDa)

9.07

Isoelectric Point (pI)

37.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Longin	PF13774	37 - 118	1.5e-25	Regulated-SNARE-like domain
Synaptobrevin	PF00957	135 - 213	1.7e-18	Synaptobrevin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0004936)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11890
fragaria_vesca	FvH4_6g35230
malus_domestica	MD09G1177400.v1.1 MD17G1149000.v1.1
prunus_persica	Prupe.3G036800_v2.0.a1 Prupe.3G036800_v2.0.a1 Prupe.3G036800_v2.0.a1
pyrus_communis	pycom09g09560 pycom17g14280
rosa_chinensis	RchiOBHm_Chr2g0091761 RchiOBHm_Chr2g0146571
rosa_laevigata	RLG00000020208
rosa_multiflora	Rmu_sc0001986.1_g000029
rosa_roxburghii	Rroxscaffold_1G00025120 Rroxscaffold_2G00091680 Rroxscaffold_2G00100030
rosa_rugosa	Rorug02G0107300 Rorug02G0107300 Rorug02G0107400 Rorug02G0107500 Rorug02G0394800 Rorug02G0394800 Rorug02G0394900 Rorug02G0395000
rosa_samantha	Rh2AG451700 Rh2BG464500 Rh2CG438600 Rh2DG473500 Rh6AG134600
rosa_wichuraiana	Rw2G036900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	593
AciI	CCGC	1 cut(s)	400
AclWI	GGATC	1 cut(s)	574
AcoI	YGGCCR	1 cut(s)	67
AcsI	RAATTY	2 cut(s)	89, 344
AfaI	GTAC	2 cut(s)	380, 590
AfiI	CCNNNNNNNGG	1 cut(s)	634
AflIII	ACRYGT	1 cut(s)	205
AgsI	TTSAA	7 cut(s)	121, 153, 167, 269, 314, 418, 565
AjiI	CACGTC	1 cut(s)	206
AjnI	CCWGG	2 cut(s)	381, 495
AluBI	AGCT	3 cut(s)	10, 427, 649
AluI	AGCT	3 cut(s)	10, 427, 649
Alw26I	GTCTC	1 cut(s)	44
AlwI	GGATC	1 cut(s)	574
AlwNI	CAGNNNCTG	1 cut(s)	577
AoxI	GGCC	1 cut(s)	67
ApeKI	GCWGC	1 cut(s)	320
ApoI	RAATTY	2 cut(s)	89, 344
ArsI	GACNNNNNNTTYG	2 cut(s)	90, 122
AspLEI	GCGC	1 cut(s)	595
AspS9I	GGNCC	3 cut(s)	173, 303, 493
AsuHPI	GGTGA	2 cut(s)	16, 494
AvaII	GGWCC	3 cut(s)	173, 303, 493
BauI	CACGAG	1 cut(s)	24
BbvI	GCAGC	1 cut(s)	307
BccI	CCATC	2 cut(s)	29, 59
BceAI	ACGGC	2 cut(s)	54, 501
BciT130I	CCWGG	2 cut(s)	383, 497
BcoDI	GTCTC	1 cut(s)	44
BfaI	CTAG	1 cut(s)	47
BglII	AGATCT	1 cut(s)	271
BisI	GCNGC	1 cut(s)	321
BlsI	GCNGC	1 cut(s)	322
Bme1390I	CCNGG	2 cut(s)	383, 497
Bme18I	GGWCC	3 cut(s)	173, 303, 493
BmgBI	CACGTC	1 cut(s)	206
BmgT120I	GGNCC	3 cut(s)	173, 303, 493
BmiI	GGNNCC	2 cut(s)	304, 305
BmrFI	CCNGG	2 cut(s)	383, 497
BpuEI	CTTGAG	1 cut(s)	260
BsaBI	GATNNNNATC	1 cut(s)	564
BsaI	GGTCTC	1 cut(s)	44
BsaWI	WCCGGW	1 cut(s)	134
Bsc4I	CCNNNNNNNGG	1 cut(s)	634
Bse1I	ACTGG	2 cut(s)	63, 175
Bse3DI	GCAATG	1 cut(s)	463
Bse8I	GATNNNNATC	1 cut(s)	564
BseBI	CCWGG	2 cut(s)	383, 497
BseGI	GGATG	2 cut(s)	67, 162
BseJI	GATNNNNATC	1 cut(s)	564
BseLI	CCNNNNNNNGG	1 cut(s)	634
BseMI	GCAATG	1 cut(s)	463
BseMII	CTCAG	1 cut(s)	410
BseNI	ACTGG	2 cut(s)	63, 175
BseXI	GCAGC	1 cut(s)	307
Bsh1236I	CGCG	1 cut(s)	593
BshFI	GGCC	1 cut(s)	69
BsiSI	CCGG	1 cut(s)	135
BslFI	GGGAC	2 cut(s)	69, 316
BslI	CCNNNNNNNGG	1 cut(s)	634
BsmAI	GTCTC	1 cut(s)	44
BsmFI	GGGAC	2 cut(s)	69, 316
BsmI	GAATGC	1 cut(s)	335
BsnI	GGCC	1 cut(s)	69
Bso31I	GGTCTC	1 cut(s)	44
Bsp143I	GATC	2 cut(s)	271, 579
BspACI	CCGC	1 cut(s)	400
BspANI	GGCC	1 cut(s)	69
BspCNI	CTCAG	1 cut(s)	409
BspFNI	CGCG	1 cut(s)	593
BspLI	GGNNCC	2 cut(s)	304, 305
BspPI	GGATC	1 cut(s)	574
BspTNI	GGTCTC	1 cut(s)	44
BsrDI	GCAATG	1 cut(s)	463
BsrI	ACTGG	2 cut(s)	63, 175
BssMI	GATC	2 cut(s)	271, 579
BssSI	CACGAG	1 cut(s)	24
Bst2BI	CACGAG	1 cut(s)	24
Bst2UI	CCWGG	2 cut(s)	383, 497
Bst4CI	ACNGT	1 cut(s)	233
BstDEI	CTNAG	1 cut(s)	396
BstF5I	GGATG	2 cut(s)	67, 162
BstFNI	CGCG	1 cut(s)	593
BstHHI	GCGC	1 cut(s)	595
BstKTI	GATC	2 cut(s)	274, 582
BstMAI	GTCTC	1 cut(s)	44
BstMBI	GATC	2 cut(s)	271, 579
BstMWI	GCNNNNNNNGC	2 cut(s)	551, 599
BstNI	CCWGG	2 cut(s)	383, 497
BstSCI	CCNGG	2 cut(s)	381, 495
BstUI	CGCG	1 cut(s)	593
BstV1I	GCAGC	1 cut(s)	307
BstX2I	RGATCY	1 cut(s)	271
BstYI	RGATCY	1 cut(s)	271
BsuRI	GGCC	1 cut(s)	69
BtrI	CACGTC	1 cut(s)	206
BtsCI	GGATG	2 cut(s)	67, 162
CaiI	CAGNNNCTG	1 cut(s)	577
CfoI	GCGC	1 cut(s)	595
Cfr13I	GGNCC	3 cut(s)	173, 303, 493
CsiI	ACCWGGT	1 cut(s)	495
Csp6I	GTAC	2 cut(s)	379, 589
CviJI	RGCY	8 cut(s)	10, 69, 113, 148, 427, 516, 575, 649
CviKI_1	RGCY	8 cut(s)	10, 69, 113, 148, 427, 516, 575, 649
CviQI	GTAC	2 cut(s)	379, 589
DdeI	CTNAG	1 cut(s)	396
DpnI	GATC	2 cut(s)	273, 581
DpnII	GATC	2 cut(s)	271, 579
EaeI	YGGCCR	1 cut(s)	67
Eco31I	GGTCTC	1 cut(s)	44
Eco47I	GGWCC	3 cut(s)	173, 303, 493
EcoO109I	RGGNCCY	1 cut(s)	303
EcoRI	GAATTC	1 cut(s)	89
EcoRII	CCWGG	2 cut(s)	381, 495
EcoT22I	ATGCAT	1 cut(s)	337
FaiI	YATR	7 cut(s)	192, 333, 359, 432, 539, 541, 543
FaqI	GGGAC	2 cut(s)	69, 316
Fnu4HI	GCNGC	1 cut(s)	321
FokI	GGATG	2 cut(s)	74, 169
Fsp4HI	GCNGC	1 cut(s)	321
FspBI	CTAG	1 cut(s)	47
GlaI	GCGC	1 cut(s)	594
GluI	GCNGC	1 cut(s)	321
HaeIII	GGCC	1 cut(s)	69
HapII	CCGG	1 cut(s)	135
HhaI	GCGC	1 cut(s)	595
Hin6I	GCGC	1 cut(s)	593
HinP1I	GCGC	1 cut(s)	593
HincII	GTYRAC	1 cut(s)	132
HindII	GTYRAC	1 cut(s)	132
HinfI	GANTC	4 cut(s)	83, 451, 530, 565
HpaII	CCGG	1 cut(s)	135
HphI	GGTGA	2 cut(s)	16, 494
Hpy166II	GTNNAC	2 cut(s)	132, 439
Hpy188I	TCNGA	4 cut(s)	88, 529, 579, 584
Hpy188III	TCNNGA	3 cut(s)	121, 277, 464
Hpy8I	GTNNAC	2 cut(s)	132, 439
HpyAV	CCTTC	3 cut(s)	159, 194, 265
HpyCH4III	ACNGT	1 cut(s)	233
HpyCH4IV	ACGT	1 cut(s)	205
HpyCH4V	TGCA	3 cut(s)	323, 335, 410
HpyF10VI	GCNNNNNNNGC	2 cut(s)	551, 599
HpyF3I	CTNAG	1 cut(s)	396
HpySE526I	ACGT	1 cut(s)	205
HspAI	GCGC	1 cut(s)	593
KflI	GGGWCCC	1 cut(s)	303
Kzo9I	GATC	2 cut(s)	271, 579
Lsp1109I	GCAGC	1 cut(s)	307
MabI	ACCWGGT	1 cut(s)	495
MaeI	CTAG	1 cut(s)	47
MaeII	ACGT	1 cut(s)	205
MaeIII	GTNAC	3 cut(s)	28, 212, 227
MalI	GATC	2 cut(s)	273, 581
MboI	GATC	2 cut(s)	271, 579
MboII	GAAGA	1 cut(s)	281
MflI	RGATCY	1 cut(s)	271
MluCI	AATT	4 cut(s)	89, 309, 344, 374
MlyI	GAGTC	2 cut(s)	77, 445
MmeI	TCCRAC	1 cut(s)	471
MnlI	CCTC	2 cut(s)	46, 635
Mph1103I	ATGCAT	1 cut(s)	337
MseI	TTAA	4 cut(s)	78, 297, 342, 521
MspA1I	CMGCKG	1 cut(s)	400
MspI	CCGG	1 cut(s)	135
MspR9I	CCNGG	2 cut(s)	383, 497
Mva1269I	GAATGC	1 cut(s)	335
MvaI	CCWGG	2 cut(s)	383, 497
MvnI	CGCG	1 cut(s)	593
MwoI	GCNNNNNNNGC	2 cut(s)	551, 599
NdeII	GATC	2 cut(s)	271, 579
NlaIV	GGNNCC	2 cut(s)	304, 305
NmuCI	GTSAC	1 cut(s)	227
NsiI	ATGCAT	1 cut(s)	337
PctI	GAATGC	1 cut(s)	335
PfeI	GAWTC	2 cut(s)	530, 565
PflFI	GACNNNGTC	1 cut(s)	497
PkrI	GCNGC	1 cut(s)	322
PleI	GAGTC	2 cut(s)	77, 445
PpsI	GAGTC	2 cut(s)	77, 445
PpuMI	RGGWCCY	1 cut(s)	303
Psp5II	RGGWCCY	1 cut(s)	303
Psp6I	CCWGG	2 cut(s)	381, 495
PspGI	CCWGG	2 cut(s)	381, 495
PspN4I	GGNNCC	2 cut(s)	304, 305
PspPI	GGNCC	3 cut(s)	173, 303, 493
PspPPI	RGGWCCY	1 cut(s)	303
PstNI	CAGNNNCTG	1 cut(s)	577
PsuI	RGATCY	1 cut(s)	271
PsyI	GACNNNGTC	1 cut(s)	497
RsaI	GTAC	2 cut(s)	380, 590
RsaNI	GTAC	2 cut(s)	379, 589
SaqAI	TTAA	4 cut(s)	78, 297, 342, 521
SatI	GCNGC	1 cut(s)	321
Sau3AI	GATC	2 cut(s)	271, 579
Sau96I	GGNCC	3 cut(s)	173, 303, 493
SchI	GAGTC	2 cut(s)	77, 445
ScrFI	CCNGG	2 cut(s)	383, 497
SetI	ASST	9 cut(s)	12, 178, 208, 219, 267, 331, 429, 501, 651
SexAI	ACCWGGT	1 cut(s)	495
SinI	GGWCC	3 cut(s)	173, 303, 493
SmlI	CTYRAG	1 cut(s)	275
SmoI	CTYRAG	1 cut(s)	275
Sse9I	AATT	4 cut(s)	89, 309, 344, 374
SsiI	CCGC	1 cut(s)	400
SspI	AATATT	1 cut(s)	406
SspMI	CTAG	1 cut(s)	47
StyD4I	CCNGG	2 cut(s)	381, 495
TaaI	ACNGT	1 cut(s)	233
TaiI	ACGT	1 cut(s)	208
TaqI	TCGA	3 cut(s)	198, 512, 568
TasI	AATT	4 cut(s)	89, 309, 344, 374
TfiI	GAWTC	2 cut(s)	530, 565
Tru1I	TTAA	4 cut(s)	78, 297, 342, 521
Tru9I	TTAA	4 cut(s)	78, 297, 342, 521
TseFI	GTSAC	1 cut(s)	227
TseI	GCWGC	1 cut(s)	320
Tsp45I	GTSAC	1 cut(s)	227
TspDTI	ATGAA	3 cut(s)	328, 346, 447
Tth111I	GACNNNGTC	1 cut(s)	497
VpaK11BI	GGWCC	3 cut(s)	173, 303, 493
XapI	RAATTY	2 cut(s)	89, 344
XspI	CTAG	1 cut(s)	47
Zsp2I	ATGCAT	1 cut(s)	337

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.