Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0004936

Physical Location & Seq

Genomic Coordinates Reverse (-)

13601973 .. 13605559

Gene Structure 3587 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1149000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 657 bp

ATGGTGAAGCTGACAATGATTGCTCGTGTTACTGATGGTCTTCCACTAGCAGAGGGACTGGATGATGGCCGTGATGTAAAAGACTCGGAATTCTACAAACAACAAGTCAAGGCTTTATTCAAGAACTTGTCAAGAGGCCAAAATGAGCCTTCAAGGATGTCAGTTGAAACTGGTCCTTTTGTTTTCCACTATATCATCGAAGGACGTGTGTGTTACTTAACAATGTGCGACCGTGCCTATCCAAAGAAACTTGCCTTCCAATACCTTGAAGATCTCAAGAATGAATTTGAGCGTGTTAACGGGACCCAAATTGAAACTGCTGCAAGGCCTTATGCATTCATTAAATTTGATACATTCATACAGAAAACAAAGAAATTGTACCAGGACACTCGCACTCAGAGGAATATTGCAAAGTTGAATGATGAGCTCTATGAAGTCCACCAAATAATGACTCGCAATGTTCAGGAAGTGCTTGGTGTTGGTGAAAAGTTGGACCAGGTTAGTCAAATGTCCAGCCGTTTAACATCAGAATCTCGTGTGTATGCTGATAAAGCGAGAGATTTGAATCGACAGGCTCTGATCCGAAAGTATGCACCTATTGCCATTGTGCTTGGAGTGGTTTTCCTCCTTTTCTGGGTCAAAACAAAGCTCTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000149 GO:0003674 GO:0005484 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0005886 GO:0006810 GO:0006887 GO:0006906 GO:0006996 GO:0008150 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0031201 GO:0032940 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0046903 GO:0048284 GO:0051179 GO:0051234 GO:0061024 GO:0061025 GO:0071840 GO:0071944 GO:0090174 GO:0098796

KEGG Pathways

Metabolic & Signaling

ko04130 ko04145 ko05134 map04130 map04145 map05134

Pfam Domains

Protein Families

Longin Synaptobrevin

Protein Analysis

219

Amino Acids

25.36

Weight (kDa)

9.3

Isoelectric Point (pI)

38.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Longin	PF13774	37 - 118	1.6e-25	Regulated-SNARE-like domain
Synaptobrevin	PF00957	135 - 213	1e-18	Synaptobrevin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0004936)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11890
fragaria_vesca	FvH4_6g35230
malus_domestica	MD09G1177400.v1.1 MD17G1149000.v1.1
prunus_persica	Prupe.3G036800_v2.0.a1 Prupe.3G036800_v2.0.a1 Prupe.3G036800_v2.0.a1
pyrus_communis	pycom09g09560 pycom17g14280
rosa_chinensis	RchiOBHm_Chr2g0091761 RchiOBHm_Chr2g0146571
rosa_laevigata	RLG00000020208
rosa_multiflora	Rmu_sc0001986.1_g000029
rosa_roxburghii	Rroxscaffold_1G00025120 Rroxscaffold_2G00091680 Rroxscaffold_2G00100030
rosa_rugosa	Rorug02G0107300 Rorug02G0107300 Rorug02G0107400 Rorug02G0107500 Rorug02G0394800 Rorug02G0394800 Rorug02G0394900 Rorug02G0395000
rosa_samantha	Rh2AG451700 Rh2BG464500 Rh2CG438600 Rh2DG473500 Rh6AG134600
rosa_wichuraiana	Rw2G036900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AclWI	GGATC	1 cut(s)	574
AcoI	YGGCCR	1 cut(s)	67
AcsI	RAATTY	3 cut(s)	89, 284, 344
AfaI	GTAC	1 cut(s)	380
AfiI	CCNNNNNNNGG	1 cut(s)	634
AflIII	ACRYGT	1 cut(s)	205
AgsI	TTSAA	7 cut(s)	121, 153, 167, 269, 314, 418, 565
AjiI	CACGTC	1 cut(s)	206
AjnI	CCWGG	2 cut(s)	381, 495
AluBI	AGCT	3 cut(s)	10, 427, 649
AluI	AGCT	3 cut(s)	10, 427, 649
Alw21I	GWGCWC	1 cut(s)	429
AlwI	GGATC	1 cut(s)	574
AlwNI	CAGNNNCTG	1 cut(s)	577
AoxI	GGCC	3 cut(s)	67, 136, 326
ApeKI	GCWGC	1 cut(s)	320
ApoI	RAATTY	3 cut(s)	89, 284, 344
ArsI	GACNNNNNNTTYG	2 cut(s)	90, 122
AspS9I	GGNCC	3 cut(s)	173, 303, 493
AsuHPI	GGTGA	2 cut(s)	16, 494
AvaII	GGWCC	3 cut(s)	173, 303, 493
BanII	GRGCYC	1 cut(s)	429
BauI	CACGAG	2 cut(s)	24, 534
BbsI	GAAGAC	1 cut(s)	32
Bbv12I	GWGCWC	1 cut(s)	429
BbvI	GCAGC	1 cut(s)	307
BccI	CCATC	2 cut(s)	29, 59
BceAI	ACGGC	2 cut(s)	54, 501
BciT130I	CCWGG	2 cut(s)	383, 497
BfaI	CTAG	1 cut(s)	47
BglII	AGATCT	1 cut(s)	271
BisI	GCNGC	1 cut(s)	321
BlsI	GCNGC	1 cut(s)	322
Bme1390I	CCNGG	2 cut(s)	383, 497
Bme18I	GGWCC	3 cut(s)	173, 303, 493
BmgBI	CACGTC	1 cut(s)	206
BmgT120I	GGNCC	3 cut(s)	173, 303, 493
BmiI	GGNNCC	2 cut(s)	304, 305
BmrFI	CCNGG	2 cut(s)	383, 497
BpiI	GAAGAC	1 cut(s)	32
BpuEI	CTTGAG	1 cut(s)	260
BsaBI	GATNNNNATC	1 cut(s)	564
Bsc4I	CCNNNNNNNGG	1 cut(s)	634
Bse1I	ACTGG	2 cut(s)	63, 175
Bse3DI	GCAATG	1 cut(s)	463
Bse8I	GATNNNNATC	1 cut(s)	564
BseBI	CCWGG	2 cut(s)	383, 497
BseGI	GGATG	2 cut(s)	67, 162
BseJI	GATNNNNATC	1 cut(s)	564
BseLI	CCNNNNNNNGG	1 cut(s)	634
BseMI	GCAATG	1 cut(s)	463
BseMII	CTCAG	1 cut(s)	410
BseNI	ACTGG	2 cut(s)	63, 175
BseXI	GCAGC	1 cut(s)	307
Bsh1285I	CGRYCG	1 cut(s)	232
BshFI	GGCC	3 cut(s)	69, 138, 328
BsiEI	CGRYCG	1 cut(s)	232
BsiHKAI	GWGCWC	1 cut(s)	429
BslFI	GGGAC	2 cut(s)	69, 316
BslI	CCNNNNNNNGG	1 cut(s)	634
BsmFI	GGGAC	2 cut(s)	69, 316
BsmI	GAATGC	1 cut(s)	335
BsnI	GGCC	3 cut(s)	69, 138, 328
Bsp1286I	GDGCHC	1 cut(s)	429
Bsp143I	GATC	2 cut(s)	271, 579
BspANI	GGCC	3 cut(s)	69, 138, 328
BspCNI	CTCAG	1 cut(s)	409
BspLI	GGNNCC	2 cut(s)	304, 305
BspPI	GGATC	1 cut(s)	574
BsrDI	GCAATG	1 cut(s)	463
BsrI	ACTGG	2 cut(s)	63, 175
BssMI	GATC	2 cut(s)	271, 579
BssSI	CACGAG	2 cut(s)	24, 534
Bst2BI	CACGAG	2 cut(s)	24, 534
Bst2UI	CCWGG	2 cut(s)	383, 497
Bst4CI	ACNGT	1 cut(s)	233
BstAPI	GCANNNNNTGC	1 cut(s)	599
BstDEI	CTNAG	1 cut(s)	396
BstF5I	GGATG	2 cut(s)	67, 162
BstKTI	GATC	2 cut(s)	274, 582
BstMBI	GATC	2 cut(s)	271, 579
BstMCI	CGRYCG	1 cut(s)	232
BstMWI	GCNNNNNNNGC	2 cut(s)	551, 599
BstNI	CCWGG	2 cut(s)	383, 497
BstSCI	CCNGG	2 cut(s)	381, 495
BstV1I	GCAGC	1 cut(s)	307
BstV2I	GAAGAC	1 cut(s)	32
BstX2I	RGATCY	1 cut(s)	271
BstYI	RGATCY	1 cut(s)	271
BsuRI	GGCC	3 cut(s)	69, 138, 328
BtrI	CACGTC	1 cut(s)	206
BtsCI	GGATG	2 cut(s)	67, 162
CaiI	CAGNNNCTG	1 cut(s)	577
Cfr13I	GGNCC	3 cut(s)	173, 303, 493
CsiI	ACCWGGT	1 cut(s)	495
Csp6I	GTAC	1 cut(s)	379
CviQI	GTAC	1 cut(s)	379
DdeI	CTNAG	1 cut(s)	396
DpnI	GATC	2 cut(s)	273, 581
DpnII	GATC	2 cut(s)	271, 579
EaeI	YGGCCR	1 cut(s)	67
Ecl136II	GAGCTC	1 cut(s)	427
Eco147I	AGGCCT	1 cut(s)	328
Eco24I	GRGCYC	1 cut(s)	429
Eco47I	GGWCC	3 cut(s)	173, 303, 493
Eco53kI	GAGCTC	1 cut(s)	427
EcoICRI	GAGCTC	1 cut(s)	427
EcoO109I	RGGNCCY	1 cut(s)	303
EcoRI	GAATTC	1 cut(s)	89
EcoRII	CCWGG	2 cut(s)	381, 495
EcoT22I	ATGCAT	1 cut(s)	337
EcoT38I	GRGCYC	1 cut(s)	429
FaiI	YATR	6 cut(s)	192, 333, 359, 432, 543, 591
FaqI	GGGAC	2 cut(s)	69, 316
Fnu4HI	GCNGC	1 cut(s)	321
FokI	GGATG	2 cut(s)	74, 169
FriOI	GRGCYC	1 cut(s)	429
Fsp4HI	GCNGC	1 cut(s)	321
FspBI	CTAG	1 cut(s)	47
GluI	GCNGC	1 cut(s)	321
HaeIII	GGCC	3 cut(s)	69, 138, 328
HincII	GTYRAC	1 cut(s)	298
HindII	GTYRAC	1 cut(s)	298
HinfI	GANTC	4 cut(s)	83, 451, 530, 565
HpaI	GTTAAC	1 cut(s)	298
HphI	GGTGA	2 cut(s)	16, 494
Hpy166II	GTNNAC	2 cut(s)	298, 439
Hpy188I	TCNGA	5 cut(s)	88, 399, 529, 579, 584
Hpy188III	TCNNGA	4 cut(s)	121, 132, 277, 464
Hpy8I	GTNNAC	2 cut(s)	298, 439
HpyAV	CCTTC	3 cut(s)	159, 194, 265
HpyCH4III	ACNGT	1 cut(s)	233
HpyCH4IV	ACGT	1 cut(s)	205
HpyCH4V	TGCA	4 cut(s)	323, 335, 410, 593
HpyF10VI	GCNNNNNNNGC	2 cut(s)	551, 599
HpyF3I	CTNAG	1 cut(s)	396
HpySE526I	ACGT	1 cut(s)	205
KflI	GGGWCCC	1 cut(s)	303
KspAI	GTTAAC	1 cut(s)	298
Kzo9I	GATC	2 cut(s)	271, 579
Lsp1109I	GCAGC	1 cut(s)	307
MabI	ACCWGGT	1 cut(s)	495
MaeI	CTAG	1 cut(s)	47
MaeII	ACGT	1 cut(s)	205
MaeIII	GTNAC	2 cut(s)	28, 212
MalI	GATC	2 cut(s)	273, 581
MboI	GATC	2 cut(s)	271, 579
MboII	GAAGA	2 cut(s)	32, 281
MflI	RGATCY	1 cut(s)	271
MhlI	GDGCHC	1 cut(s)	429
MluCI	AATT	5 cut(s)	89, 284, 309, 344, 374
MlyI	GAGTC	2 cut(s)	77, 445
MmeI	TCCRAC	1 cut(s)	471
MnlI	CCTC	4 cut(s)	46, 128, 393, 635
Mph1103I	ATGCAT	1 cut(s)	337
MseI	TTAA	4 cut(s)	218, 297, 342, 521
MspR9I	CCNGG	2 cut(s)	383, 497
Mva1269I	GAATGC	1 cut(s)	335
MvaI	CCWGG	2 cut(s)	383, 497
MwoI	GCNNNNNNNGC	2 cut(s)	551, 599
NdeII	GATC	2 cut(s)	271, 579
NlaIV	GGNNCC	2 cut(s)	304, 305
NsiI	ATGCAT	1 cut(s)	337
PceI	AGGCCT	1 cut(s)	328
PctI	GAATGC	1 cut(s)	335
PfeI	GAWTC	2 cut(s)	530, 565
PkrI	GCNGC	1 cut(s)	322
PleI	GAGTC	2 cut(s)	77, 445
PpsI	GAGTC	2 cut(s)	77, 445
PpuMI	RGGWCCY	1 cut(s)	303
Psp124BI	GAGCTC	1 cut(s)	429
Psp5II	RGGWCCY	1 cut(s)	303
Psp6I	CCWGG	2 cut(s)	381, 495
PspGI	CCWGG	2 cut(s)	381, 495
PspN4I	GGNNCC	2 cut(s)	304, 305
PspPI	GGNCC	3 cut(s)	173, 303, 493
PspPPI	RGGWCCY	1 cut(s)	303
PstNI	CAGNNNCTG	1 cut(s)	577
PsuI	RGATCY	1 cut(s)	271
RsaI	GTAC	1 cut(s)	380
RsaNI	GTAC	1 cut(s)	379
SacI	GAGCTC	1 cut(s)	429
SaqAI	TTAA	4 cut(s)	218, 297, 342, 521
SatI	GCNGC	1 cut(s)	321
Sau3AI	GATC	2 cut(s)	271, 579
Sau96I	GGNCC	3 cut(s)	173, 303, 493
SchI	GAGTC	2 cut(s)	77, 445
ScrFI	CCNGG	2 cut(s)	383, 497
SduI	GDGCHC	1 cut(s)	429
SetI	ASST	7 cut(s)	12, 208, 267, 429, 501, 598, 651
SexAI	ACCWGGT	1 cut(s)	495
SinI	GGWCC	3 cut(s)	173, 303, 493
SmlI	CTYRAG	1 cut(s)	275
SmoI	CTYRAG	1 cut(s)	275
Sse9I	AATT	5 cut(s)	89, 284, 309, 344, 374
SseBI	AGGCCT	1 cut(s)	328
SspI	AATATT	1 cut(s)	406
SspMI	CTAG	1 cut(s)	47
SstI	GAGCTC	1 cut(s)	429
StuI	AGGCCT	1 cut(s)	328
StyD4I	CCNGG	2 cut(s)	381, 495
TaaI	ACNGT	1 cut(s)	233
TaiI	ACGT	1 cut(s)	208
TaqI	TCGA	2 cut(s)	198, 568
TasI	AATT	5 cut(s)	89, 284, 309, 344, 374
TfiI	GAWTC	2 cut(s)	530, 565
Tru1I	TTAA	4 cut(s)	218, 297, 342, 521
Tru9I	TTAA	4 cut(s)	218, 297, 342, 521
TseI	GCWGC	1 cut(s)	320
TspDTI	ATGAA	4 cut(s)	297, 328, 346, 447
VpaK11BI	GGWCC	3 cut(s)	173, 303, 493
XapI	RAATTY	3 cut(s)	89, 284, 344
XspI	CTAG	1 cut(s)	47
Zsp2I	ATGCAT	1 cut(s)	337

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.