Rorug02G0107400

25.3 kDa vesicle transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004936
Physical Location & Seq
Forward (+)
9160703 .. 9163607
2905 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0107400.1

Sequence Viewer

Length: 1245 bp
ATGGCAAGTCAATCGGAGGAAGAAGCTGTTCCTATGGAGAAGAGGCCCGGAATCTTGTTCATCGGATCACCCACCGTCGGCAAAAGAACTCTCATCTCTCGATTACTGGGTTTGGACTTGGACGATGGTTCGGATTCTTCACCGTCTCAGTTGGCGGTTCATGGGTGGAGTATCAATACTAAGTACTACACAGCTGATGTGGCTGTGTGGATGGCTCATCTTGATGAGCAGTTTTCGATTGAGAAGGTTCCAATGTATGATCAGCTAGTTGCTTTGATTATGGTGTTTGATACAACTGAGTTGTCATCTCTTGTTGCACTTCAGAAATGGGTGTCGCGCAATGATCTTCAGAAATTCGATATATTGTTGTGCATAGGGAATAAAGTTGATCTTGTGCCAGGTCACCCAGTTCATGTGGAGTATAGGAGACGTCTGCTGAAGATTGGGGAGCCTTTTGCTGATTCCCAGTCGGATGTTACTGAGTATGGAATCTCTGAAACTGATGGGAGCAGTTTACTGGGGGAGGATGAACCATCTTGGGAGGCTAGAGGGTCGTGTTTGGAATGGTGCACGGAGAATAACATTGAGTACATTGAAGCTTGTGGTTCGAATGCTGAGTTTGACAAATGTTTGTCAGTTGATGGCGATTCACAAGGTGTTGAACGACTCTTTGGTGCTCTTTCTGCACATATGTGGCCTGGAATGATTCTGAAAACAGGCGATAAGATTACTGAACCATCATTACCTGAAAGAGAAGACTCGTCAGAAGAAGAATCTGATTATGAATTCGAGTATGATGTCCTATCGGCGGGTTCAGCTGAACCTTTTGATGACACAGATAAAGGATGGGGATGGGTTTCTGCCAGTGATGTTGCTGGAACCTCAGAAATGGGAAGACTGGATGCTCATAATAATCTTGTCCAAGAGAATGGAACTACTAGCGCTAATGAGGAGATGCATGCCTCTACATCAACGGCTGCATTACATAGTGATATCAACAAGGAAGTAGTGGTGCCCAATGCGGAAGAACCTGACCGCGACACAGAACTTGATCAACGCCAAGAGTTAGATGAGGATACACCTTTCGAAATTGAGGATTTGGAACAGTTGATGTCTGAAATCGGGAACATGCGTGACAGCTTGAGATTGATGCCTGATTTTCAAAGACGAGAAATGGCTGCAAAACTGGCCATGAAAATGGCATCCATGTTTGGGGGCGAGAGTGATAATGAACAGGAAAGTTGA

Protein Analysis

414

Amino Acids

46.01

Weight (kDa)

4.23

Isoelectric Point (pI)

64.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_binding PF10199 216 - 398 2.7e-16 Alpha and gamma adaptin binding protein p34
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 433
AccB1I GGYRCC 1 cut(s) 1012
AccII CGCG 2 cut(s) 337, 1038
AciI CCGC 4 cut(s) 155, 809, 1022, 1036
AclWI GGATC 1 cut(s) 73
AcoI YGGCCR 1 cut(s) 1188
AcsI RAATTY 2 cut(s) 353, 785
AcuI CTGAAG 3 cut(s) 305, 332, 458
AcyI GRCGYC 1 cut(s) 430
AdeI CACNNNGTG 1 cut(s) 656
AfaI GTAC 2 cut(s) 185, 590
AfeI AGCGCT 1 cut(s) 943
AfiI CCNNNNNNNGG 3 cut(s) 77, 808, 1212
AgsI TTSAA 3 cut(s) 596, 662, 1163
AjnI CCWGG 2 cut(s) 397, 697
AjuI GAANNNNNNNTTGG 2 cut(s) 654, 686
AleI CACNNNNGTG 1 cut(s) 691
AluBI AGCT 6 cut(s) 26, 194, 265, 599, 818, 1140
AluI AGCT 6 cut(s) 26, 194, 265, 599, 818, 1140
Alw21I GWGCWC 2 cut(s) 572, 679
Alw26I GTCTC 2 cut(s) 150, 421
Alw44I GTGCAC 1 cut(s) 568
AlwI GGATC 1 cut(s) 73
Aor51HI AGCGCT 1 cut(s) 943
AoxI GGCC 3 cut(s) 44, 695, 1188
ApaLI GTGCAC 1 cut(s) 568
ApeKI GCWGC 2 cut(s) 977, 1178
ApoI RAATTY 2 cut(s) 353, 785
Asp700I GAANNNNTTC 1 cut(s) 27
AspLEI GCGC 2 cut(s) 339, 944
AspS9I GGNCC 1 cut(s) 45
AsuC2I CCSGG 1 cut(s) 48
AsuHPI GGTGA 3 cut(s) 60, 132, 395
AsuII TTCGAA 2 cut(s) 608, 1086
BaeGI GKGCMC 2 cut(s) 572, 1017
BalI TGGCCA 1 cut(s) 1190
BanI GGYRCC 1 cut(s) 1012
BbsI GAAGAC 2 cut(s) 762, 901
Bbv12I GWGCWC 2 cut(s) 572, 679
BbvI GCAGC 2 cut(s) 964, 1165
BccI CCATC 8 cut(s) 119, 205, 497, 541, 635, 745, 840, 846
BceAI ACGGC 1 cut(s) 990
BcgI CGANNNNNNTGC 1 cut(s) 28
BciT130I CCWGG 2 cut(s) 399, 699
BciVI GTATCC 1 cut(s) 1069
BclI TGATCA 2 cut(s) 259, 1051
BcnI CCSGG 1 cut(s) 48
BcoDI GTCTC 2 cut(s) 150, 421
BfaI CTAG 3 cut(s) 266, 546, 939
BfoI RGCGCY 1 cut(s) 945
BfuI GTATCC 1 cut(s) 1069
BisI GCNGC 2 cut(s) 978, 1179
BlsI GCNGC 2 cut(s) 979, 1180
BmcAI AGTACT 1 cut(s) 185
Bme1390I CCNGG 3 cut(s) 48, 399, 699
BmgT120I GGNCC 1 cut(s) 45
BmiI GGNNCC 4 cut(s) 249, 450, 880, 1014
BmrFI CCNGG 3 cut(s) 48, 399, 699
BmrI ACTGGG 4 cut(s) 116, 401, 460, 527
BmsI GCATC 4 cut(s) 892, 945, 1140, 1211
BmuI ACTGGG 4 cut(s) 116, 401, 460, 527
BpiI GAAGAC 2 cut(s) 762, 901
Bpu14I TTCGAA 2 cut(s) 608, 1086
BpuEI CTTGAG 1 cut(s) 1162
BpuMI CCSGG 1 cut(s) 48
BsaHI GRCGYC 1 cut(s) 430
Bsc4I CCNNNNNNNGG 3 cut(s) 77, 808, 1212
Bse1I ACTGG 7 cut(s) 111, 407, 466, 522, 864, 903, 1191
Bse3DI GCAATG 1 cut(s) 346
BseBI CCWGG 2 cut(s) 399, 699
BseGI GGATG 7 cut(s) 216, 478, 532, 851, 857, 907, 1202
BseLI CCNNNNNNNGG 3 cut(s) 77, 808, 1212
BseMI GCAATG 1 cut(s) 346
BseMII CTCAG 5 cut(s) 161, 288, 471, 606, 897
BseNI ACTGG 7 cut(s) 111, 407, 466, 522, 864, 903, 1191
BseRI GAGGAG 1 cut(s) 965
BseSI GKGCMC 2 cut(s) 572, 1017
BseXI GCAGC 2 cut(s) 964, 1165
BsgI GTGCAG 1 cut(s) 669
Bsh1236I CGCG 2 cut(s) 337, 1038
BshFI GGCC 3 cut(s) 46, 697, 1190
BshNI GGYRCC 1 cut(s) 1012
BsiHKAI GWGCWC 2 cut(s) 572, 679
BsiSI CCGG 1 cut(s) 48
BslI CCNNNNNNNGG 3 cut(s) 77, 808, 1212
BsmAI GTCTC 2 cut(s) 150, 421
BsmBI CGTCTC 2 cut(s) 150, 421
BsmI GAATGC 1 cut(s) 616
BsnI GGCC 3 cut(s) 46, 697, 1190
Bsp119I TTCGAA 2 cut(s) 608, 1086
Bsp1286I GDGCHC 3 cut(s) 572, 679, 1017
Bsp143I GATC 5 cut(s) 65, 259, 343, 388, 1051
BspACI CCGC 4 cut(s) 155, 809, 1022, 1036
BspANI GGCC 3 cut(s) 46, 697, 1190
BspCNI CTCAG 5 cut(s) 160, 289, 472, 607, 896
BspFNI CGCG 2 cut(s) 337, 1038
BspLI GGNNCC 4 cut(s) 249, 450, 880, 1014
BspPI GGATC 1 cut(s) 73
BspT104I TTCGAA 2 cut(s) 608, 1086
BspT107I GGYRCC 1 cut(s) 1012
BsrDI GCAATG 1 cut(s) 346
BsrI ACTGG 7 cut(s) 111, 407, 466, 522, 864, 903, 1191
BssMI GATC 5 cut(s) 65, 259, 343, 388, 1051
BssNI GRCGYC 1 cut(s) 430
Bst2UI CCWGG 2 cut(s) 399, 699
Bst4CI ACNGT 3 cut(s) 76, 144, 1107
Bst6I CTCTTC 1 cut(s) 35
BstACI GRCGYC 1 cut(s) 430
BstBI TTCGAA 2 cut(s) 608, 1086
BstC8I GCNNGC 1 cut(s) 960
BstDEI CTNAG 6 cut(s) 147, 180, 297, 480, 615, 883
BstEII GGTNACC 1 cut(s) 401
BstF5I GGATG 7 cut(s) 216, 478, 532, 851, 857, 907, 1202
BstFNI CGCG 2 cut(s) 337, 1038
BstH2I RGCGCY 1 cut(s) 945
BstHHI GCGC 2 cut(s) 339, 944
BstKTI GATC 5 cut(s) 68, 262, 346, 391, 1054
BstMAI GTCTC 2 cut(s) 150, 421
BstMBI GATC 5 cut(s) 65, 259, 343, 388, 1051
BstMWI GCNNNNNNNGC 4 cut(s) 200, 683, 815, 1187
BstNI CCWGG 2 cut(s) 399, 699
BstNSI RCATGY 2 cut(s) 962, 1132
BstPI GGTNACC 1 cut(s) 401
BstSCI CCNGG 3 cut(s) 46, 397, 697
BstSLI GKGCMC 2 cut(s) 572, 1017
BstUI CGCG 2 cut(s) 337, 1038
BstV1I GCAGC 2 cut(s) 964, 1165
BstV2I GAAGAC 2 cut(s) 762, 901
BstXI CCANNNNNNTGG 2 cut(s) 929, 1198
BsuI GTATCC 1 cut(s) 1069
BsuRI GGCC 3 cut(s) 46, 697, 1190
BtsCI GGATG 7 cut(s) 216, 478, 532, 851, 857, 907, 1202
BtsIMutI CAGTG 1 cut(s) 871
Cac8I GCNNGC 1 cut(s) 960
CfoI GCGC 2 cut(s) 339, 944
Cfr13I GGNCC 1 cut(s) 45
Csp6I GTAC 2 cut(s) 184, 589
CviAII CATG 6 cut(s) 161, 413, 959, 1129, 1192, 1207
CviQI GTAC 2 cut(s) 184, 589
DdeI CTNAG 6 cut(s) 147, 180, 297, 480, 615, 883
DpnI GATC 5 cut(s) 67, 261, 345, 390, 1053
DpnII GATC 5 cut(s) 65, 259, 343, 388, 1051
DraIII CACNNNGTG 1 cut(s) 656
EaeI YGGCCR 1 cut(s) 1188
Eam1104I CTCTTC 1 cut(s) 35
EarI CTCTTC 1 cut(s) 35
Eco32I GATATC 1 cut(s) 994
Eco47III AGCGCT 1 cut(s) 943
Eco57I CTGAAG 3 cut(s) 305, 332, 458
Eco91I GGTNACC 1 cut(s) 401
EcoO65I GGTNACC 1 cut(s) 401
EcoRI GAATTC 1 cut(s) 785
EcoRII CCWGG 2 cut(s) 397, 697
EcoRV GATATC 1 cut(s) 994
EcoT22I ATGCAT 1 cut(s) 960
Esp3I CGTCTC 2 cut(s) 150, 421
FaeI CATG 6 cut(s) 164, 416, 962, 1132, 1195, 1210
FalI AAGNNNNNCTT 2 cut(s) 375, 407
FatI CATG 6 cut(s) 160, 412, 958, 1128, 1191, 1206
FauI CCCGC 1 cut(s) 802
FauNDI CATATG 1 cut(s) 690
FbaI TGATCA 2 cut(s) 259, 1051
Fnu4HI GCNGC 2 cut(s) 978, 1179
FokI GGATG 7 cut(s) 223, 485, 539, 858, 864, 914, 1189
Fsp4HI GCNGC 2 cut(s) 978, 1179
FspBI CTAG 3 cut(s) 266, 546, 939
GlaI GCGC 2 cut(s) 338, 943
GluI GCNGC 2 cut(s) 978, 1179
HaeII RGCGCY 1 cut(s) 945
HaeIII GGCC 3 cut(s) 46, 697, 1190
HapII CCGG 1 cut(s) 48
HhaI GCGC 2 cut(s) 339, 944
Hin1I GRCGYC 1 cut(s) 430
Hin1II CATG 6 cut(s) 164, 416, 962, 1132, 1195, 1210
Hin6I GCGC 2 cut(s) 337, 942
HinP1I GCGC 2 cut(s) 337, 942
HindIII AAGCTT 1 cut(s) 597
HinfI GANTC 9 cut(s) 51, 134, 461, 489, 647, 666, 706, 758, 773
HpaII CCGG 1 cut(s) 48
HphI GGTGA 3 cut(s) 60, 132, 395
Hpy166II GTNNAC 2 cut(s) 515, 570
Hpy188III TCNNGA 3 cut(s) 99, 221, 1123
Hpy8I GTNNAC 2 cut(s) 515, 570
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 1 cut(s) 238
HpyCH4III ACNGT 3 cut(s) 76, 144, 1107
HpyCH4IV ACGT 1 cut(s) 430
HpyCH4V TGCA 7 cut(s) 317, 372, 570, 686, 958, 980, 1181
HpyF10VI GCNNNNNNNGC 4 cut(s) 200, 683, 815, 1187
HpyF3I CTNAG 6 cut(s) 147, 180, 297, 480, 615, 883
HpySE526I ACGT 1 cut(s) 430
Hsp92I GRCGYC 1 cut(s) 430
Hsp92II CATG 6 cut(s) 164, 416, 962, 1132, 1195, 1210
HspAI GCGC 2 cut(s) 337, 942
Ksp22I TGATCA 2 cut(s) 259, 1051
Kzo9I GATC 5 cut(s) 65, 259, 343, 388, 1051
LmnI GCTCC 2 cut(s) 448, 507
Lsp1109I GCAGC 2 cut(s) 964, 1165
LweI GCATC 4 cut(s) 892, 945, 1140, 1211
MaeI CTAG 3 cut(s) 266, 546, 939
MaeII ACGT 1 cut(s) 430
MaeIII GTNAC 3 cut(s) 401, 475, 1133
MalI GATC 5 cut(s) 67, 261, 345, 390, 1053
MboI GATC 5 cut(s) 65, 259, 343, 388, 1051
MhlI GDGCHC 3 cut(s) 572, 679, 1017
MlsI TGGCCA 1 cut(s) 1190
MluCI AATT 3 cut(s) 353, 785, 1089
MluNI TGGCCA 1 cut(s) 1190
MlyI GAGTC 2 cut(s) 660, 752
MmeI TCCRAC 1 cut(s) 450
Mox20I TGGCCA 1 cut(s) 1190
Mph1103I ATGCAT 1 cut(s) 960
MroXI GAANNNNTTC 1 cut(s) 27
MscI TGGCCA 1 cut(s) 1190
MslI CAYNNNNRTG 3 cut(s) 222, 691, 1196
Msp20I TGGCCA 1 cut(s) 1190
MspA1I CMGCKG 2 cut(s) 194, 818
MspI CCGG 1 cut(s) 48
MspR9I CCNGG 3 cut(s) 48, 399, 699
Mva1269I GAATGC 1 cut(s) 616
MvaI CCWGG 2 cut(s) 399, 699
MvnI CGCG 2 cut(s) 337, 1038
MwoI GCNNNNNNNGC 4 cut(s) 200, 683, 815, 1187
NciI CCSGG 1 cut(s) 48
NdeI CATATG 1 cut(s) 690
NdeII GATC 5 cut(s) 65, 259, 343, 388, 1051
NlaIII CATG 6 cut(s) 164, 416, 962, 1132, 1195, 1210
NlaIV GGNNCC 4 cut(s) 249, 450, 880, 1014
NmuCI GTSAC 2 cut(s) 401, 1133
NsiI ATGCAT 1 cut(s) 960
NspI RCATGY 2 cut(s) 962, 1132
NspV TTCGAA 2 cut(s) 608, 1086
OliI CACNNNNGTG 1 cut(s) 691
PaeI GCATGC 1 cut(s) 962
PctI GAATGC 1 cut(s) 616
PdmI GAANNNNTTC 1 cut(s) 27
PfeI GAWTC 7 cut(s) 51, 134, 461, 489, 647, 706, 773
PkrI GCNGC 2 cut(s) 979, 1180
PleI GAGTC 2 cut(s) 660, 752
PpsI GAGTC 2 cut(s) 660, 752
Psp6I CCWGG 2 cut(s) 397, 697
PspEI GGTNACC 1 cut(s) 401
PspGI CCWGG 2 cut(s) 397, 697
PspN4I GGNNCC 4 cut(s) 249, 450, 880, 1014
PspPI GGNCC 1 cut(s) 45
PsrI GAACNNNNNNTAC 2 cut(s) 726, 758
PvuII CAGCTG 2 cut(s) 194, 818
RsaI GTAC 2 cut(s) 185, 590
RsaNI GTAC 2 cut(s) 184, 589
RseI CAYNNNNRTG 3 cut(s) 222, 691, 1196
SatI GCNGC 2 cut(s) 978, 1179
Sau3AI GATC 5 cut(s) 65, 259, 343, 388, 1051
Sau96I GGNCC 1 cut(s) 45
ScaI AGTACT 1 cut(s) 185
SchI GAGTC 2 cut(s) 660, 752
ScrFI CCNGG 3 cut(s) 48, 399, 699
SduI GDGCHC 3 cut(s) 572, 679, 1017
SfaNI GCATC 4 cut(s) 892, 945, 1140, 1211
SfuI TTCGAA 2 cut(s) 608, 1086
SmiMI CAYNNNNRTG 3 cut(s) 222, 691, 1196
SmlI CTYRAG 1 cut(s) 1141
SmoI CTYRAG 1 cut(s) 1141
SphI GCATGC 1 cut(s) 962
Sse9I AATT 3 cut(s) 353, 785, 1089
SsiI CCGC 4 cut(s) 155, 809, 1022, 1036
SspMI CTAG 3 cut(s) 266, 546, 939
StyD4I CCNGG 3 cut(s) 46, 397, 697
TaaI ACNGT 3 cut(s) 76, 144, 1107
TaiI ACGT 1 cut(s) 433
TaqI TCGA 6 cut(s) 100, 236, 357, 608, 789, 1086
TasI AATT 3 cut(s) 353, 785, 1089
TatI WGTACW 2 cut(s) 183, 588
TfiI GAWTC 7 cut(s) 51, 134, 461, 489, 647, 706, 773
TscAI CASTG 1 cut(s) 871
TseFI GTSAC 2 cut(s) 401, 1133
TseI GCWGC 2 cut(s) 977, 1178
Tsp45I GTSAC 2 cut(s) 401, 1133
TspDTI ATGAA 7 cut(s) 49, 149, 401, 543, 798, 1208, 1245
TspGWI ACGGA 1 cut(s) 587
TspRI CASTG 1 cut(s) 871
VneI GTGCAC 1 cut(s) 568
XapI RAATTY 2 cut(s) 353, 785
XceI RCATGY 2 cut(s) 962, 1132
XmnI GAANNNNTTC 1 cut(s) 27
XspI CTAG 3 cut(s) 266, 546, 939
ZraI GACGTC 1 cut(s) 431
ZrmI AGTACT 1 cut(s) 185
Zsp2I ATGCAT 1 cut(s) 960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.