Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0002512

Physical Location & Seq

Genomic Coordinates Forward (+)

20202573 .. 20206446

Gene Structure 3874 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1209700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 942 bp

ATGGAGTCCGTCGGCGTCCTCATGCCAGTCCCAATGTCCCCGTACTTAGAGCAAGAGCTCGAGAGGCGCTTCAACCTCCTCAAGCTCTGGACGGCCCCTGAAAAAGCGGAGTTCCTCAAGGCCCACTCCGGCGCCGTCCGAGCCGTCATCGGCAATGCTGGGAACGGAGCCGACGTCGGGCTCATCGAGGCGCTGCCGGAGCTGGAGATCATCGCCAGCTTCAGCGTCGGAATCGATAAGGTGGATTTGGAGAAGTGTAGGGAAAAGGGTATTCGGGTCACGAACACGCCGGACGTTTTGACCGACGACGTGGCGGACCTTGCGATTGGGTTGGCGTTGGGTGTGCTGCGACGGCTCTGTGAGGCTGACGGGTATGTGAGGAGTGGGGGGTGGAAGAACGGCGACTACAAGTTGACCACCAAGTTCACTGGAAAAACTGTGGGCATCATCGGATTGGGAAGAATTGGAAAAGCAGTTGCCAAGAGAGTCGAGGCTTTTAGCTGTCCAATCGCATACTACTCCAGAACAGAAAAACCAGATTTAAAGTACAAATACTATCCAAGTGTTGTGGAGTTGGCCTCCAACTGTGATATCCTGGTTGTTGTATGCCCGTTAACTCAAGAGACCCGCCACATTATTAATCGTGAAGTCATTGATGCTTTGGGTCCAAAGGGTATTCTCATTAACATTGGGAGGGGTCCTCATGTTGATGAATCTGAGCTGGTATCTGCACTGCTAGAAGGCCGGTTAGGTGGTGCTGGGCTCGATGTTTATGAAAATGAACCAGATGTACCTGAGCAGCTGTTTCAGCTCGAGAATGTGGTACTCTTACCTCATGTAGGAAGTGACACTGTGGAAACAGACACTGCCATGGCTGACCTTGTGATTGGGAATCTTGAGGCTCATTTTTTGAACAAGCCATTGTTGACGCCGGTGGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0008152 GO:0009853 GO:0009854 GO:0009987 GO:0016491 GO:0016614 GO:0016616 GO:0016618 GO:0030267 GO:0043094 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0055114

KEGG Pathways

Metabolic & Signaling

ko00130 ko00260 ko00350 ko00360 ko00630 ko00960 ko01100 ko01110 ko01130 ko01200 map00130 map00260 map00350 map00360 map00630 map00960 map01100 map01110 map01130 map01200

Pfam Domains

Protein Families

2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

314

Amino Acids

33.94

Weight (kDa)

5.01

Isoelectric Point (pI)

31.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
2-Hacid_dh	PF00389	13 - 312	1.1e-22	D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C	PF02826	109 - 281	5.8e-55	D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002512)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79870 AT1G79870
fragaria_vesca	FvH4_6g31711 FvH4_6g31720 FvH4_6g31721
malus_domestica	MD09G1209700.v1.1 MD09G1209800.v1.1 MD17G1191700.v1.1
prunus_persica	Prupe.3G067700_v2.0.a1 Prupe.3G067700_v2.0.a1 Prupe.3G067700_v2.0.a1 Prupe.3G067800_v2.0.a1
pyrus_communis	pycom09g12600 pycom09g12630 pycom17g19870
rosa_chinensis	RchiOBHm_Chr2g0138801 RchiOBHm_Chr2g0138891 RchiOBHm_Chr2g0138911
rosa_laevigata	RLG00000019761 RLG00000019763 RLG00000019764
rosa_multiflora	Rmu_co8474001.1_g000001 Rmu_sc0010783.1_g000009 Rmu_sc0011444.1_g000002 Rmu_sc0016387.1_g000002 Rmu_sc0017407.1_g000002
rosa_roxburghii	Rroxscaffold_2G00105920 Rroxscaffold_2G00105950
rosa_rugosa	Rorug02G0352600 Rorug02G0352800 Rorug02G0352900
rosa_samantha	Rh2AG402500 Rh2AG403000 Rh2BG411600 Rh2CG388600 Rh2DG422700 Rh2DG423100 Rh2DG423200
rosa_wichuraiana	Rw2G032720 Rw2G032780 Rw2G032790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	177
AccB1I	GGYRCC	1 cut(s)	131
AciI	CCGC	3 cut(s)	107, 314, 628
AcuI	CTGAAG	1 cut(s)	205
AcyI	GRCGYC	4 cut(s)	15, 132, 174, 929
AfaI	GTAC	4 cut(s)	44, 548, 792, 825
AfiI	CCNNNNNNNGG	2 cut(s)	177, 839
AgsI	TTSAA	2 cut(s)	73, 913
AjiI	CACGTC	1 cut(s)	310
AjnI	CCWGG	1 cut(s)	594
AluBI	AGCT	8 cut(s)	58, 85, 202, 219, 501, 721, 802, 811
AluI	AGCT	8 cut(s)	58, 85, 202, 219, 501, 721, 802, 811
Alw21I	GWGCWC	1 cut(s)	60
Alw26I	GTCTC	1 cut(s)	617
AlwNI	CAGNNNCTG	1 cut(s)	866
Ama87I	CYCGRG	2 cut(s)	59, 812
AoxI	GGCC	4 cut(s)	93, 120, 576, 742
ApeKI	GCWGC	3 cut(s)	193, 346, 799
AseI	ATTAAT	1 cut(s)	639
AspLEI	GCGC	3 cut(s)	69, 134, 193
AspS9I	GGNCC	5 cut(s)	94, 121, 316, 665, 698
AvaI	CYCGRG	2 cut(s)	59, 812
AvaII	GGWCC	3 cut(s)	316, 665, 698
BanI	GGYRCC	1 cut(s)	131
BanII	GRGCYC	3 cut(s)	60, 183, 765
Bbv12I	GWGCWC	1 cut(s)	60
BbvI	GCAGC	3 cut(s)	180, 333, 811
BceAI	ACGGC	5 cut(s)	108, 119, 128, 368, 415
BciT130I	CCWGG	1 cut(s)	596
BcoDI	GTCTC	1 cut(s)	617
BfaI	CTAG	1 cut(s)	737
BfoI	RGCGCY	3 cut(s)	70, 135, 194
BisI	GCNGC	3 cut(s)	194, 347, 800
BlsI	GCNGC	3 cut(s)	195, 348, 801
Bme1390I	CCNGG	1 cut(s)	596
Bme18I	GGWCC	3 cut(s)	316, 665, 698
BmeT110I	CYCGRG	2 cut(s)	59, 812
BmgBI	CACGTC	1 cut(s)	310
BmgT120I	GGNCC	5 cut(s)	94, 121, 316, 665, 698
BmiI	GGNNCC	5 cut(s)	96, 133, 169, 666, 699
BmrFI	CCNGG	1 cut(s)	596
BmsI	GCATC	2 cut(s)	453, 646
BplI	GAGNNNNNCTC	4 cut(s)	563, 595, 685, 717
BpmI	CTGGAG	2 cut(s)	224, 505
Bpu10I	CCTNAGC	1 cut(s)	795
BpuEI	CTTGAG	4 cut(s)	65, 101, 603, 917
Bsa29I	ATCGAT	1 cut(s)	234
BsaHI	GRCGYC	4 cut(s)	15, 132, 174, 929
BsaI	GGTCTC	1 cut(s)	617
BsaJI	CCNNGG	1 cut(s)	870
BsaXI	ACNNNNNCTCC	2 cut(s)	159, 189
Bsc4I	CCNNNNNNNGG	2 cut(s)	177, 839
Bse118I	RCCGGY	2 cut(s)	744, 931
Bse1I	ACTGG	2 cut(s)	26, 433
Bse3DI	GCAATG	1 cut(s)	160
BseBI	CCWGG	1 cut(s)	596
BseCI	ATCGAT	1 cut(s)	234
BseDI	CCNNGG	1 cut(s)	870
BseLI	CCNNNNNNNGG	2 cut(s)	177, 839
BseMI	GCAATG	1 cut(s)	160
BseMII	CTCAG	2 cut(s)	708, 786
BseNI	ACTGG	2 cut(s)	26, 433
BseRI	GAGGAG	2 cut(s)	68, 394
BseXI	GCAGC	3 cut(s)	180, 333, 811
BseYI	CCCAGC	2 cut(s)	158, 758
BsgI	GTGCAG	1 cut(s)	714
BshFI	GGCC	4 cut(s)	95, 122, 578, 744
BshNI	GGYRCC	1 cut(s)	131
BshVI	ATCGAT	1 cut(s)	234
BsiHKAI	GWGCWC	1 cut(s)	60
BsiHKCI	CYCGRG	2 cut(s)	59, 812
BsiSI	CCGG	5 cut(s)	129, 197, 290, 745, 932
BslFI	GGGAC	2 cut(s)	14, 22
BslI	CCNNNNNNNGG	2 cut(s)	177, 839
BsmAI	GTCTC	1 cut(s)	617
BsmFI	GGGAC	2 cut(s)	14, 22
BsnI	GGCC	4 cut(s)	95, 122, 578, 744
Bso31I	GGTCTC	1 cut(s)	617
BsoBI	CYCGRG	2 cut(s)	59, 812
Bsp1286I	GDGCHC	3 cut(s)	60, 183, 765
Bsp143I	GATC	1 cut(s)	207
Bsp19I	CCATGG	1 cut(s)	870
BspACI	CCGC	3 cut(s)	107, 314, 628
BspANI	GGCC	4 cut(s)	95, 122, 578, 744
BspCNI	CTCAG	2 cut(s)	709, 787
BspDI	ATCGAT	1 cut(s)	234
BspLI	GGNNCC	5 cut(s)	96, 133, 169, 666, 699
BspT107I	GGYRCC	1 cut(s)	131
BspTNI	GGTCTC	1 cut(s)	617
BsrDI	GCAATG	1 cut(s)	160
BsrFI	RCCGGY	2 cut(s)	744, 931
BsrI	ACTGG	2 cut(s)	26, 433
BssAI	RCCGGY	2 cut(s)	744, 931
BssECI	CCNNGG	1 cut(s)	870
BssMI	GATC	1 cut(s)	207
BssNI	GRCGYC	4 cut(s)	15, 132, 174, 929
BssT1I	CCWWGG	1 cut(s)	870
Bst2UI	CCWGG	1 cut(s)	596
Bst4CI	ACNGT	3 cut(s)	439, 587, 853
BstACI	GRCGYC	4 cut(s)	15, 132, 174, 929
BstC8I	GCNNGC	1 cut(s)	217
BstDEI	CTNAG	3 cut(s)	46, 717, 795
BstDSI	CCRYGG	1 cut(s)	870
BstENI	CCTNNNNNAGG	1 cut(s)	837
BstH2I	RGCGCY	3 cut(s)	70, 135, 194
BstHHI	GCGC	3 cut(s)	69, 134, 193
BstKTI	GATC	1 cut(s)	210
BstMAI	GTCTC	1 cut(s)	617
BstMBI	GATC	1 cut(s)	207
BstMWI	GCNNNNNNNGC	6 cut(s)	64, 140, 199, 320, 352, 808
BstNI	CCWGG	1 cut(s)	596
BstSCI	CCNGG	1 cut(s)	594
BstV1I	GCAGC	3 cut(s)	180, 333, 811
Bsu15I	ATCGAT	1 cut(s)	234
BsuRI	GGCC	4 cut(s)	95, 122, 578, 744
BsuTUI	ATCGAT	1 cut(s)	234
BtgI	CCRYGG	1 cut(s)	870
BtgZI	GCGATG	1 cut(s)	196
BtrI	CACGTC	1 cut(s)	310
BtsI	GCAGTG	2 cut(s)	731, 864
BtsIMutI	CAGTG	4 cut(s)	426, 731, 849, 864
Cac8I	GCNNGC	1 cut(s)	217
CaiI	CAGNNNCTG	1 cut(s)	866
CfoI	GCGC	3 cut(s)	69, 134, 193
Cfr10I	RCCGGY	2 cut(s)	744, 931
Cfr13I	GGNCC	5 cut(s)	94, 121, 316, 665, 698
ClaI	ATCGAT	1 cut(s)	234
CseI	GACGC	3 cut(s)	4, 214, 937
Csp6I	GTAC	4 cut(s)	43, 547, 791, 824
CspCI	CAANNNNNGTGG	2 cut(s)	549, 584
CviAII	CATG	4 cut(s)	22, 704, 836, 871
CviQI	GTAC	4 cut(s)	43, 547, 791, 824
DdeI	CTNAG	3 cut(s)	46, 717, 795
DinI	GGCGCC	1 cut(s)	133
DpnI	GATC	1 cut(s)	209
DpnII	GATC	1 cut(s)	207
DraI	TTTAAA	1 cut(s)	543
EciI	GGCGGA	1 cut(s)	329
Ecl136II	GAGCTC	1 cut(s)	58
Eco130I	CCWWGG	1 cut(s)	870
Eco24I	GRGCYC	3 cut(s)	60, 183, 765
Eco31I	GGTCTC	1 cut(s)	617
Eco32I	GATATC	1 cut(s)	592
Eco47I	GGWCC	3 cut(s)	316, 665, 698
Eco53kI	GAGCTC	1 cut(s)	58
Eco57I	CTGAAG	1 cut(s)	205
Eco88I	CYCGRG	2 cut(s)	59, 812
EcoICRI	GAGCTC	1 cut(s)	58
EcoNI	CCTNNNNNAGG	1 cut(s)	837
EcoO109I	RGGNCCY	1 cut(s)	698
EcoRII	CCWGG	1 cut(s)	594
EcoRV	GATATC	1 cut(s)	592
EcoT14I	CCWWGG	1 cut(s)	870
EcoT38I	GRGCYC	3 cut(s)	60, 183, 765
EgeI	GGCGCC	1 cut(s)	133
EheI	GGCGCC	1 cut(s)	133
ErhI	CCWWGG	1 cut(s)	870
FaeI	CATG	4 cut(s)	25, 707, 839, 874
FaiI	YATR	8 cut(s)	23, 375, 514, 607, 705, 774, 837, 872
FaqI	GGGAC	2 cut(s)	14, 22
FatI	CATG	4 cut(s)	21, 703, 835, 870
FauI	CCCGC	1 cut(s)	635
Fnu4HI	GCNGC	3 cut(s)	194, 347, 800
FriOI	GRGCYC	3 cut(s)	60, 183, 765
Fsp4HI	GCNGC	3 cut(s)	194, 347, 800
FspBI	CTAG	1 cut(s)	737
GlaI	GCGC	3 cut(s)	68, 133, 192
GluI	GCNGC	3 cut(s)	194, 347, 800
GsaI	CCCAGC	2 cut(s)	162, 762
GsuI	CTGGAG	2 cut(s)	224, 505
HaeII	RGCGCY	3 cut(s)	70, 135, 194
HaeIII	GGCC	4 cut(s)	95, 122, 578, 744
HapII	CCGG	5 cut(s)	129, 197, 290, 745, 932
HgaI	GACGC	3 cut(s)	4, 214, 937
HhaI	GCGC	3 cut(s)	69, 134, 193
Hin1I	GRCGYC	4 cut(s)	15, 132, 174, 929
Hin1II	CATG	4 cut(s)	25, 707, 839, 874
Hin6I	GCGC	3 cut(s)	67, 132, 191
HinP1I	GCGC	3 cut(s)	67, 132, 191
HincII	GTYRAC	3 cut(s)	414, 615, 927
HindII	GTYRAC	3 cut(s)	414, 615, 927
HinfI	GANTC	5 cut(s)	5, 231, 486, 713, 892
HpaI	GTTAAC	1 cut(s)	615
HpaII	CCGG	5 cut(s)	129, 197, 290, 745, 932
Hpy166II	GTNNAC	4 cut(s)	414, 426, 615, 927
Hpy188I	TCNGA	4 cut(s)	140, 230, 452, 718
Hpy188III	TCNNGA	8 cut(s)	61, 88, 280, 522, 620, 644, 814, 896
Hpy8I	GTNNAC	4 cut(s)	414, 426, 615, 927
Hpy99I	CGWCG	7 cut(s)	14, 176, 179, 230, 308, 311, 354
HpyAV	CCTTC	1 cut(s)	734
HpyCH4III	ACNGT	3 cut(s)	439, 587, 853
HpyCH4IV	ACGT	3 cut(s)	174, 294, 309
HpyCH4V	TGCA	1 cut(s)	731
HpyF10VI	GCNNNNNNNGC	6 cut(s)	64, 140, 199, 320, 352, 808
HpyF3I	CTNAG	3 cut(s)	46, 717, 795
HpySE526I	ACGT	3 cut(s)	174, 294, 309
Hsp92I	GRCGYC	4 cut(s)	15, 132, 174, 929
Hsp92II	CATG	4 cut(s)	25, 707, 839, 874
HspAI	GCGC	3 cut(s)	67, 132, 191
KasI	GGCGCC	1 cut(s)	131
KspAI	GTTAAC	1 cut(s)	615
Kzo9I	GATC	1 cut(s)	207
LmnI	GCTCC	2 cut(s)	167, 199
Lsp1109I	GCAGC	3 cut(s)	180, 333, 811
LweI	GCATC	2 cut(s)	453, 646
MaeI	CTAG	1 cut(s)	737
MaeII	ACGT	3 cut(s)	174, 294, 309
MaeIII	GTNAC	2 cut(s)	277, 845
MalI	GATC	1 cut(s)	209
MboI	GATC	1 cut(s)	207
MboII	GAAGA	2 cut(s)	406, 471
MhlI	GDGCHC	3 cut(s)	60, 183, 765
MluCI	AATT	1 cut(s)	462
Mly113I	GGCGCC	1 cut(s)	132
MlyI	GAGTC	2 cut(s)	14, 495
MmeI	TCCRAC	2 cut(s)	208, 606
MseI	TTAA	5 cut(s)	542, 614, 639, 684, 940
MslI	CAYNNNNRTG	2 cut(s)	708, 869
MspA1I	CMGCKG	1 cut(s)	802
MspI	CCGG	5 cut(s)	129, 197, 290, 745, 932
MspR9I	CCNGG	1 cut(s)	596
MvaI	CCWGG	1 cut(s)	596
MwoI	GCNNNNNNNGC	6 cut(s)	64, 140, 199, 320, 352, 808
NarI	GGCGCC	1 cut(s)	132
NcoI	CCATGG	1 cut(s)	870
NdeII	GATC	1 cut(s)	207
NlaIII	CATG	4 cut(s)	25, 707, 839, 874
NlaIV	GGNNCC	5 cut(s)	96, 133, 169, 666, 699
NmuCI	GTSAC	2 cut(s)	277, 845
PaeR7I	CTCGAG	2 cut(s)	59, 812
PcsI	WCGNNNNNNNCGW	3 cut(s)	171, 183, 300
PfeI	GAWTC	3 cut(s)	231, 713, 892
PkrI	GCNGC	3 cut(s)	195, 348, 801
PleI	GAGTC	2 cut(s)	13, 494
PluTI	GGCGCC	1 cut(s)	135
PpsI	GAGTC	2 cut(s)	13, 494
PpuMI	RGGWCCY	1 cut(s)	698
PshBI	ATTAAT	1 cut(s)	639
Psp124BI	GAGCTC	1 cut(s)	60
Psp5II	RGGWCCY	1 cut(s)	698
Psp6I	CCWGG	1 cut(s)	594
PspFI	CCCAGC	2 cut(s)	158, 758
PspGI	CCWGG	1 cut(s)	594
PspN4I	GGNNCC	5 cut(s)	96, 133, 169, 666, 699
PspPI	GGNCC	5 cut(s)	94, 121, 316, 665, 698
PspPPI	RGGWCCY	1 cut(s)	698
PsrI	GAACNNNNNNTAC	4 cut(s)	389, 421, 774, 806
PstNI	CAGNNNCTG	1 cut(s)	866
PvuII	CAGCTG	1 cut(s)	802
RsaI	GTAC	4 cut(s)	44, 548, 792, 825
RsaNI	GTAC	4 cut(s)	43, 547, 791, 824
RseI	CAYNNNNRTG	2 cut(s)	708, 869
SacI	GAGCTC	1 cut(s)	60
SaqAI	TTAA	5 cut(s)	542, 614, 639, 684, 940
SatI	GCNGC	3 cut(s)	194, 347, 800
Sau3AI	GATC	1 cut(s)	207
Sau96I	GGNCC	5 cut(s)	94, 121, 316, 665, 698
SchI	GAGTC	2 cut(s)	14, 495
ScrFI	CCNGG	1 cut(s)	596
SduI	GDGCHC	3 cut(s)	60, 183, 765
SfaNI	GCATC	2 cut(s)	453, 646
SfoI	GGCGCC	1 cut(s)	133
Sfr274I	CTCGAG	2 cut(s)	59, 812
SgrAI	CRCCGGYG	1 cut(s)	931
SinI	GGWCC	3 cut(s)	316, 665, 698
SlaI	CTCGAG	2 cut(s)	59, 812
SmiMI	CAYNNNNRTG	2 cut(s)	708, 869
SmlI	CTYRAG	6 cut(s)	59, 80, 116, 618, 812, 896
SmoI	CTYRAG	6 cut(s)	59, 80, 116, 618, 812, 896
Sse9I	AATT	1 cut(s)	462
SsiI	CCGC	3 cut(s)	107, 314, 628
SspDI	GGCGCC	1 cut(s)	131
SspMI	CTAG	1 cut(s)	737
SstI	GAGCTC	1 cut(s)	60
StyD4I	CCNGG	1 cut(s)	594
StyI	CCWWGG	1 cut(s)	870
TaaI	ACNGT	3 cut(s)	439, 587, 853
TaiI	ACGT	3 cut(s)	177, 297, 312
TaqI	TCGA	6 cut(s)	60, 186, 234, 489, 765, 813
TaqII	GACCGA	1 cut(s)	317
TasI	AATT	1 cut(s)	462
TatI	WGTACW	1 cut(s)	546
TfiI	GAWTC	3 cut(s)	231, 713, 892
Tru1I	TTAA	5 cut(s)	542, 614, 639, 684, 940
Tru9I	TTAA	5 cut(s)	542, 614, 639, 684, 940
TscAI	CASTG	4 cut(s)	433, 738, 856, 871
TseFI	GTSAC	2 cut(s)	277, 845
TseI	GCWGC	3 cut(s)	193, 346, 799
Tsp45I	GTSAC	2 cut(s)	277, 845
TspDTI	ATGAA	3 cut(s)	726, 789, 795
TspGWI	ACGGA	1 cut(s)	180
TspRI	CASTG	4 cut(s)	433, 738, 856, 871
VpaK11BI	GGWCC	3 cut(s)	316, 665, 698
VspI	ATTAAT	1 cut(s)	639
XagI	CCTNNNNNAGG	1 cut(s)	837
XhoI	CTCGAG	2 cut(s)	59, 812
XspI	CTAG	1 cut(s)	737
ZraI	GACGTC	1 cut(s)	175

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.