Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0002512

Physical Location & Seq

Genomic Coordinates Forward (+)

22855130 .. 22857659

Gene Structure 2530 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1191700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 942 bp

ATGGAGTCCATCGCCGTCCTCATGCCGATCCCAATGTCCTCCTACTTGGAGCAGGAGCTCGAAAAGCGCTTCAACCTCCTCAAGCTCTGGACCGTCCCCAATAAAACCCAGTTCATCAAGGACCACTCCGCGTCCATCCGTGCCATCGTCGGCAACGCCTCCGCGGGAGCCGACGCCGAGCTCATCGCCGCTCTGCCTAAGCTGGAGATTGTTTCCAGTTTCAGCGTTGGGATCGATAAGGTCGATTTGAAGACGTGTAAGGAAAAGGGTATTCGGGTCACCAACACCCCCGACGTTTTGACCGACGACGTGGCGGACCTTGCTATTGGGCTGACGTTGGCGGTGCTGAGACGGCTGTGTGAGAGTGATCGGTATGTGAGGAGTGGGCAGTGGAAGGAGGGCGACTACAAGTTGACCACCAAGTTCACTGGGAAAACTGTTGGCATCATTGGTCTCGGAAGAATTGGCAAGGCAGTTGCAAAGAGAGCCGAGGCTTTTAGCTGCCCAATTGCTTACTTTTCCAGGACAGAAAAGCCAGATTTGAAGTTCAAATACTACCCAAGTGTTGTGGAGTTGGCCGCCAACTGTGATGTTCTAGTTGTTGCGTGCCCCTTAACTGAAGAGACCCGCCACATTATCAATCGCGAAGTCATTAATGCTTTGGGGCCAAAGGGTGTTCTCATCAACGTTGGGAGAGGCCCTCATGTTGACGAACCTGAGCTGGTATCTGCTCTGGTAGAAGGCCGGTTAGGTGGGGCAGGGCTTGACGTTTATGAGAAGGAACCAGAAGTACCTGAAGAGCTGTTTGCGCTCGAGAATGTGGTCCTCTTGCCTCATGTCGGAAGTGGCACGGTAGAAACTCGCAGTACCATGGCAGACCTTGTGATTGGGAATCTTGAGGCTCACTTCTTGAAGAAACCATTGTTGACACCAGTGGTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0008152 GO:0009853 GO:0009854 GO:0009987 GO:0016491 GO:0016614 GO:0016616 GO:0016618 GO:0030267 GO:0043094 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0055114

KEGG Pathways

Metabolic & Signaling

ko00130 ko00260 ko00350 ko00360 ko00630 ko00960 ko01100 ko01110 ko01130 ko01200 map00130 map00260 map00350 map00360 map00630 map00960 map01100 map01110 map01130 map01200

Pfam Domains

Protein Families

2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

314

Amino Acids

34.07

Weight (kDa)

5.86

Isoelectric Point (pI)

28.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
2-Hacid_dh	PF00389	14 - 312	9.3e-25	D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C	PF02826	109 - 281	6.7e-56	D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002512)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79870 AT1G79870
fragaria_vesca	FvH4_6g31711 FvH4_6g31720 FvH4_6g31721
malus_domestica	MD09G1209700.v1.1 MD09G1209800.v1.1 MD17G1191700.v1.1
prunus_persica	Prupe.3G067700_v2.0.a1 Prupe.3G067700_v2.0.a1 Prupe.3G067700_v2.0.a1 Prupe.3G067800_v2.0.a1
pyrus_communis	pycom09g12600 pycom09g12630 pycom17g19870
rosa_chinensis	RchiOBHm_Chr2g0138801 RchiOBHm_Chr2g0138891 RchiOBHm_Chr2g0138911
rosa_laevigata	RLG00000019761 RLG00000019763 RLG00000019764
rosa_multiflora	Rmu_co8474001.1_g000001 Rmu_sc0010783.1_g000009 Rmu_sc0011444.1_g000002 Rmu_sc0016387.1_g000002 Rmu_sc0017407.1_g000002
rosa_roxburghii	Rroxscaffold_2G00105920 Rroxscaffold_2G00105950
rosa_rugosa	Rorug02G0352600 Rorug02G0352800 Rorug02G0352900
rosa_samantha	Rh2AG402500 Rh2AG403000 Rh2BG411600 Rh2CG388600 Rh2DG422700 Rh2DG423100 Rh2DG423200
rosa_wichuraiana	Rw2G032720 Rw2G032780 Rw2G032790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	191
AccII	CGCG	3 cut(s)	131, 164, 645
AciI	CCGC	8 cut(s)	129, 162, 164, 189, 314, 341, 579, 628
AclI	AACGTT	1 cut(s)	687
AclWI	GGATC	2 cut(s)	22, 239
AcoI	YGGCCR	1 cut(s)	576
AcuI	CTGAAG	2 cut(s)	639, 816
AcyI	GRCGYC	1 cut(s)	174
AfaI	GTAC	2 cut(s)	792, 868
AfeI	AGCGCT	1 cut(s)	68
AfiI	CCNNNNNNNGG	1 cut(s)	839
AflIII	ACRYGT	1 cut(s)	254
AgsI	TTSAA	5 cut(s)	73, 250, 544, 550, 913
AjiI	CACGTC	2 cut(s)	255, 310
AjnI	CCWGG	1 cut(s)	521
AluBI	AGCT	7 cut(s)	58, 85, 181, 202, 501, 721, 802
AluI	AGCT	7 cut(s)	58, 85, 181, 202, 501, 721, 802
Alw21I	GWGCWC	2 cut(s)	60, 183
Alw26I	GTCTC	3 cut(s)	343, 458, 617
AlwI	GGATC	2 cut(s)	22, 239
Ama87I	CYCGRG	1 cut(s)	812
Aor51HI	AGCGCT	1 cut(s)	68
AoxI	GGCC	4 cut(s)	576, 665, 697, 742
ApeKI	GCWGC	1 cut(s)	501
AseI	ATTAAT	1 cut(s)	654
AspLEI	GCGC	2 cut(s)	69, 811
AspS9I	GGNCC	6 cut(s)	90, 121, 316, 665, 698, 823
AsuHPI	GGTGA	1 cut(s)	271
AvaI	CYCGRG	1 cut(s)	812
AvaII	GGWCC	4 cut(s)	90, 121, 316, 823
BaeGI	GKGCMC	1 cut(s)	611
BanII	GRGCYC	2 cut(s)	60, 183
BarI	GAAGNNNNNNTAC	2 cut(s)	536, 568
BbsI	GAAGAC	1 cut(s)	257
Bbv12I	GWGCWC	2 cut(s)	60, 183
BbvI	GCAGC	1 cut(s)	488
BccI	CCATC	3 cut(s)	17, 143, 152
BceAI	ACGGC	1 cut(s)	368
BciT130I	CCWGG	1 cut(s)	523
BcoDI	GTCTC	3 cut(s)	343, 458, 617
BfaI	CTAG	1 cut(s)	596
BfoI	RGCGCY	1 cut(s)	70
BisI	GCNGC	3 cut(s)	189, 502, 579
BlsI	GCNGC	3 cut(s)	190, 503, 580
Bme1390I	CCNGG	1 cut(s)	523
Bme18I	GGWCC	4 cut(s)	90, 121, 316, 823
BmeT110I	CYCGRG	1 cut(s)	812
BmgBI	CACGTC	2 cut(s)	255, 310
BmgT120I	GGNCC	6 cut(s)	90, 121, 316, 665, 698, 823
BmiI	GGNNCC	3 cut(s)	169, 666, 783
BmrFI	CCNGG	1 cut(s)	523
BmrI	ACTGGG	2 cut(s)	103, 438
BmsI	GCATC	1 cut(s)	453
BmuI	ACTGGG	2 cut(s)	103, 438
BpiI	GAAGAC	1 cut(s)	257
BplI	GAGNNNNNCTC	2 cut(s)	685, 717
BpmI	CTGGAG	1 cut(s)	224
Bpu10I	CCTNAGC	2 cut(s)	198, 717
BpuEI	CTTGAG	2 cut(s)	65, 917
Bsa29I	ATCGAT	1 cut(s)	234
BsaHI	GRCGYC	1 cut(s)	174
BsaI	GGTCTC	2 cut(s)	458, 617
BsaJI	CCNNGG	3 cut(s)	162, 489, 870
Bsc4I	CCNNNNNNNGG	1 cut(s)	839
Bse118I	RCCGGY	1 cut(s)	744
Bse1I	ACTGG	4 cut(s)	109, 216, 433, 932
BseBI	CCWGG	1 cut(s)	523
BseCI	ATCGAT	1 cut(s)	234
BseDI	CCNNGG	3 cut(s)	162, 489, 870
BseGI	GGATG	1 cut(s)	135
BseLI	CCNNNNNNNGG	1 cut(s)	839
BseMII	CTCAG	2 cut(s)	338, 708
BseNI	ACTGG	4 cut(s)	109, 216, 433, 932
BseRI	GAGGAG	2 cut(s)	68, 394
BseSI	GKGCMC	1 cut(s)	611
BseXI	GCAGC	1 cut(s)	488
Bsh1236I	CGCG	3 cut(s)	131, 164, 645
BshFI	GGCC	4 cut(s)	578, 667, 699, 744
BshVI	ATCGAT	1 cut(s)	234
BsiHKAI	GWGCWC	2 cut(s)	60, 183
BsiHKCI	CYCGRG	1 cut(s)	812
BsiSI	CCGG	1 cut(s)	745
BslFI	GGGAC	1 cut(s)	80
BslI	CCNNNNNNNGG	1 cut(s)	839
BsmAI	GTCTC	3 cut(s)	343, 458, 617
BsmBI	CGTCTC	1 cut(s)	343
BsmFI	GGGAC	1 cut(s)	80
BsnI	GGCC	4 cut(s)	578, 667, 699, 744
Bso31I	GGTCTC	2 cut(s)	458, 617
BsoBI	CYCGRG	1 cut(s)	812
Bsp1286I	GDGCHC	3 cut(s)	60, 183, 611
Bsp143I	GATC	3 cut(s)	27, 231, 367
Bsp19I	CCATGG	1 cut(s)	870
Bsp68I	TCGCGA	1 cut(s)	645
BspACI	CCGC	8 cut(s)	129, 162, 164, 189, 314, 341, 579, 628
BspANI	GGCC	4 cut(s)	578, 667, 699, 744
BspCNI	CTCAG	2 cut(s)	339, 709
BspDI	ATCGAT	1 cut(s)	234
BspFNI	CGCG	3 cut(s)	131, 164, 645
BspLI	GGNNCC	3 cut(s)	169, 666, 783
BspPI	GGATC	2 cut(s)	22, 239
BspQI	GCTCTTC	1 cut(s)	792
BspTNI	GGTCTC	2 cut(s)	458, 617
BsrBI	CCGCTC	1 cut(s)	191
BsrFI	RCCGGY	1 cut(s)	744
BsrI	ACTGG	4 cut(s)	109, 216, 433, 932
BssAI	RCCGGY	1 cut(s)	744
BssECI	CCNNGG	3 cut(s)	162, 489, 870
BssMI	GATC	3 cut(s)	27, 231, 367
BssNI	GRCGYC	1 cut(s)	174
BssT1I	CCWWGG	1 cut(s)	870
Bst2UI	CCWGG	1 cut(s)	523
Bst4CI	ACNGT	4 cut(s)	94, 439, 587, 853
Bst6I	CTCTTC	2 cut(s)	615, 792
BstACI	GRCGYC	1 cut(s)	174
BstC8I	GCNNGC	1 cut(s)	607
BstDEI	CTNAG	3 cut(s)	198, 347, 717
BstDSI	CCRYGG	2 cut(s)	162, 870
BstEII	GGTNACC	1 cut(s)	277
BstF5I	GGATG	1 cut(s)	135
BstFNI	CGCG	3 cut(s)	131, 164, 645
BstH2I	RGCGCY	1 cut(s)	70
BstHHI	GCGC	2 cut(s)	69, 811
BstKTI	GATC	3 cut(s)	30, 234, 370
BstMAI	GTCTC	3 cut(s)	343, 458, 617
BstMBI	GATC	3 cut(s)	27, 231, 367
BstMWI	GCNNNNNNNGC	5 cut(s)	64, 320, 352, 485, 808
BstNI	CCWGG	1 cut(s)	523
BstPI	GGTNACC	1 cut(s)	277
BstSCI	CCNGG	1 cut(s)	521
BstSLI	GKGCMC	1 cut(s)	611
BstUI	CGCG	3 cut(s)	131, 164, 645
BstV1I	GCAGC	1 cut(s)	488
BstV2I	GAAGAC	1 cut(s)	257
Bsu15I	ATCGAT	1 cut(s)	234
BsuRI	GGCC	4 cut(s)	578, 667, 699, 744
BsuTUI	ATCGAT	1 cut(s)	234
BtgI	CCRYGG	2 cut(s)	162, 870
BtgZI	GCGATG	1 cut(s)	169
BtrI	CACGTC	2 cut(s)	255, 310
BtsCI	GGATG	1 cut(s)	135
BtsI	GCAGTG	1 cut(s)	395
BtsIMutI	CAGTG	3 cut(s)	395, 426, 939
BtuMI	TCGCGA	1 cut(s)	645
Cac8I	GCNNGC	1 cut(s)	607
CfoI	GCGC	2 cut(s)	69, 811
Cfr10I	RCCGGY	1 cut(s)	744
Cfr13I	GGNCC	6 cut(s)	90, 121, 316, 665, 698, 823
Cfr42I	CCGCGG	1 cut(s)	165
ClaI	ATCGAT	1 cut(s)	234
CseI	GACGC	2 cut(s)	120, 182
Csp6I	GTAC	2 cut(s)	791, 867
CspCI	CAANNNNNGTGG	2 cut(s)	549, 584
CviAII	CATG	4 cut(s)	22, 704, 836, 871
CviQI	GTAC	2 cut(s)	791, 867
DdeI	CTNAG	3 cut(s)	198, 347, 717
DpnI	GATC	3 cut(s)	29, 233, 369
DpnII	GATC	3 cut(s)	27, 231, 367
EaeI	YGGCCR	1 cut(s)	576
Eam1104I	CTCTTC	2 cut(s)	615, 792
EarI	CTCTTC	2 cut(s)	615, 792
EciI	GGCGGA	1 cut(s)	329
Ecl136II	GAGCTC	2 cut(s)	58, 181
Eco130I	CCWWGG	1 cut(s)	870
Eco24I	GRGCYC	2 cut(s)	60, 183
Eco31I	GGTCTC	2 cut(s)	458, 617
Eco47I	GGWCC	4 cut(s)	90, 121, 316, 823
Eco47III	AGCGCT	1 cut(s)	68
Eco53kI	GAGCTC	2 cut(s)	58, 181
Eco57I	CTGAAG	2 cut(s)	639, 816
Eco88I	CYCGRG	1 cut(s)	812
Eco91I	GGTNACC	1 cut(s)	277
EcoICRI	GAGCTC	2 cut(s)	58, 181
EcoO109I	RGGNCCY	1 cut(s)	698
EcoO65I	GGTNACC	1 cut(s)	277
EcoRII	CCWGG	1 cut(s)	521
EcoT14I	CCWWGG	1 cut(s)	870
EcoT38I	GRGCYC	2 cut(s)	60, 183
ErhI	CCWWGG	1 cut(s)	870
Esp3I	CGTCTC	1 cut(s)	343
FaeI	CATG	4 cut(s)	25, 707, 839, 874
FaiI	YATR	6 cut(s)	23, 375, 705, 774, 837, 872
FaqI	GGGAC	1 cut(s)	80
FatI	CATG	4 cut(s)	21, 703, 835, 870
FauI	CCCGC	2 cut(s)	157, 635
Fnu4HI	GCNGC	3 cut(s)	189, 502, 579
FokI	GGATG	1 cut(s)	122
FriOI	GRGCYC	2 cut(s)	60, 183
Fsp4HI	GCNGC	3 cut(s)	189, 502, 579
FspBI	CTAG	1 cut(s)	596
GlaI	GCGC	2 cut(s)	68, 810
GluI	GCNGC	3 cut(s)	189, 502, 579
GsuI	CTGGAG	1 cut(s)	224
HaeII	RGCGCY	1 cut(s)	70
HaeIII	GGCC	4 cut(s)	578, 667, 699, 744
HapII	CCGG	1 cut(s)	745
HgaI	GACGC	2 cut(s)	120, 182
HhaI	GCGC	2 cut(s)	69, 811
Hin1I	GRCGYC	1 cut(s)	174
Hin1II	CATG	4 cut(s)	25, 707, 839, 874
Hin6I	GCGC	2 cut(s)	67, 809
HinP1I	GCGC	2 cut(s)	67, 809
HincII	GTYRAC	3 cut(s)	414, 709, 927
HindII	GTYRAC	3 cut(s)	414, 709, 927
HinfI	GANTC	2 cut(s)	5, 892
HpaII	CCGG	1 cut(s)	745
HphI	GGTGA	1 cut(s)	271
Hpy166II	GTNNAC	4 cut(s)	414, 426, 709, 927
Hpy188I	TCNGA	2 cut(s)	458, 842
Hpy188III	TCNNGA	5 cut(s)	88, 644, 814, 896, 910
Hpy8I	GTNNAC	4 cut(s)	414, 426, 709, 927
Hpy99I	CGWCG	5 cut(s)	152, 176, 296, 308, 311
HpyAV	CCTTC	3 cut(s)	388, 734, 772
HpyCH4III	ACNGT	4 cut(s)	94, 439, 587, 853
HpyCH4IV	ACGT	6 cut(s)	254, 294, 309, 335, 687, 768
HpyCH4V	TGCA	1 cut(s)	479
HpyF10VI	GCNNNNNNNGC	5 cut(s)	64, 320, 352, 485, 808
HpyF3I	CTNAG	3 cut(s)	198, 347, 717
HpySE526I	ACGT	6 cut(s)	254, 294, 309, 335, 687, 768
Hsp92I	GRCGYC	1 cut(s)	174
Hsp92II	CATG	4 cut(s)	25, 707, 839, 874
HspAI	GCGC	2 cut(s)	67, 809
KspI	CCGCGG	1 cut(s)	165
Kzo9I	GATC	3 cut(s)	27, 231, 367
LguI	GCTCTTC	1 cut(s)	792
LmnI	GCTCC	3 cut(s)	49, 55, 167
Lsp1109I	GCAGC	1 cut(s)	488
LweI	GCATC	1 cut(s)	453
MaeI	CTAG	1 cut(s)	596
MaeII	ACGT	6 cut(s)	254, 294, 309, 335, 687, 768
MaeIII	GTNAC	1 cut(s)	277
MalI	GATC	3 cut(s)	29, 233, 369
MbiI	CCGCTC	1 cut(s)	191
MboI	GATC	3 cut(s)	27, 231, 367
MboII	GAAGA	5 cut(s)	262, 471, 632, 809, 925
MfeI	CAATTG	1 cut(s)	507
MhlI	GDGCHC	3 cut(s)	60, 183, 611
MluCI	AATT	2 cut(s)	462, 507
MlyI	GAGTC	1 cut(s)	14
MmeI	TCCRAC	1 cut(s)	820
MseI	TTAA	2 cut(s)	614, 654
MspA1I	CMGCKG	1 cut(s)	164
MspI	CCGG	1 cut(s)	745
MspR9I	CCNGG	1 cut(s)	523
MunI	CAATTG	1 cut(s)	507
MvaI	CCWGG	1 cut(s)	523
MvnI	CGCG	3 cut(s)	131, 164, 645
MwoI	GCNNNNNNNGC	5 cut(s)	64, 320, 352, 485, 808
NcoI	CCATGG	1 cut(s)	870
NdeII	GATC	3 cut(s)	27, 231, 367
NlaIII	CATG	4 cut(s)	25, 707, 839, 874
NlaIV	GGNNCC	3 cut(s)	169, 666, 783
NmeAIII	GCCGAG	2 cut(s)	202, 514
NmuCI	GTSAC	1 cut(s)	277
NruI	TCGCGA	1 cut(s)	645
PaeR7I	CTCGAG	1 cut(s)	812
PciSI	GCTCTTC	1 cut(s)	792
PcsI	WCGNNNNNNNCGW	2 cut(s)	240, 300
PfeI	GAWTC	1 cut(s)	892
PfoI	TCCNGGA	1 cut(s)	521
PkrI	GCNGC	3 cut(s)	190, 503, 580
PleI	GAGTC	1 cut(s)	13
PpsI	GAGTC	1 cut(s)	13
PshBI	ATTAAT	1 cut(s)	654
Psp124BI	GAGCTC	2 cut(s)	60, 183
Psp1406I	AACGTT	1 cut(s)	687
Psp6I	CCWGG	1 cut(s)	521
PspEI	GGTNACC	1 cut(s)	277
PspGI	CCWGG	1 cut(s)	521
PspN4I	GGNNCC	3 cut(s)	169, 666, 783
PspPI	GGNCC	6 cut(s)	90, 121, 316, 665, 698, 823
PsrI	GAACNNNNNNTAC	2 cut(s)	774, 806
RruI	TCGCGA	1 cut(s)	645
RsaI	GTAC	2 cut(s)	792, 868
RsaNI	GTAC	2 cut(s)	791, 867
SacI	GAGCTC	2 cut(s)	60, 183
SacII	CCGCGG	1 cut(s)	165
SapI	GCTCTTC	1 cut(s)	792
SaqAI	TTAA	2 cut(s)	614, 654
SatI	GCNGC	3 cut(s)	189, 502, 579
Sau3AI	GATC	3 cut(s)	27, 231, 367
Sau96I	GGNCC	6 cut(s)	90, 121, 316, 665, 698, 823
SchI	GAGTC	1 cut(s)	14
ScrFI	CCNGG	1 cut(s)	523
SduI	GDGCHC	3 cut(s)	60, 183, 611
SfaNI	GCATC	1 cut(s)	453
Sfr274I	CTCGAG	1 cut(s)	812
Sfr303I	CCGCGG	1 cut(s)	165
SgrBI	CCGCGG	1 cut(s)	165
SinI	GGWCC	4 cut(s)	90, 121, 316, 823
SlaI	CTCGAG	1 cut(s)	812
SmlI	CTYRAG	3 cut(s)	80, 812, 896
SmoI	CTYRAG	3 cut(s)	80, 812, 896
Sse9I	AATT	2 cut(s)	462, 507
SsiI	CCGC	8 cut(s)	129, 162, 164, 189, 314, 341, 579, 628
SspMI	CTAG	1 cut(s)	596
SstI	GAGCTC	2 cut(s)	60, 183
StyD4I	CCNGG	1 cut(s)	521
StyI	CCWWGG	1 cut(s)	870
TaaI	ACNGT	4 cut(s)	94, 439, 587, 853
TaiI	ACGT	6 cut(s)	257, 297, 312, 338, 690, 771
TaqI	TCGA	4 cut(s)	60, 234, 243, 813
TaqII	GACCGA	1 cut(s)	317
TasI	AATT	2 cut(s)	462, 507
TauI	GCSGC	2 cut(s)	191, 581
TfiI	GAWTC	1 cut(s)	892
Tru1I	TTAA	2 cut(s)	614, 654
Tru9I	TTAA	2 cut(s)	614, 654
TscAI	CASTG	3 cut(s)	395, 433, 939
TseFI	GTSAC	1 cut(s)	277
TseI	GCWGC	1 cut(s)	501
Tsp45I	GTSAC	1 cut(s)	277
TspDTI	ATGAA	1 cut(s)	103
TspGWI	ACGGA	1 cut(s)	128
TspRI	CASTG	3 cut(s)	395, 433, 939
VpaK11BI	GGWCC	4 cut(s)	90, 121, 316, 823
VspI	ATTAAT	1 cut(s)	654
XhoI	CTCGAG	1 cut(s)	812
XspI	CTAG	1 cut(s)	596

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.