Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0002512

Physical Location & Seq

Genomic Coordinates Forward (+)

20214985 .. 20217613

Gene Structure 2629 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1209800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1128 bp

ATGTTATTTTTGAAAGATTTTAGAAAAAGTGACATTTATGACATTTCTGAAAGCTCCACCCATCGCTACCAAAAGCTTCCCATAATCCCACGACCACCACCTTCTCAAATCCCATCCTCTCCAATCCTTACGGGGACGGCACAAACCCCGAAACCAAACAAGCCCCATTCTCCAAATCCCACAACAATGGAGTCCATCGGCGTCCTCATGCCGATCCCCATGTCCTCCTACTTGGAGCAGGAGCTTGAGAAGCGCTTCAACCTCCTCAAGCCCTGGACTGTCCCCCACAAAACCCAGTTCATCAAGGACCACTCCACCTCCATCCGCGCCATCGTCGGCAACGCCTCCGCCGGAGCCGACGCCGAGCTCATCGCCGCTCTGCCGAAGCTGGAGATTATTTCCAGTTTCAGCGTCGGAATCGATAAGGTCGATTTGAAGACGTGTAAGGAAAAGGGTATTCGGGTCACCAACACGCCCGACGTTTTGACTGATGACGTGGCGGACCTTGCTATTGGGCTGACGTTGGCGGTGCTGAGACGGCTCTGTGAGAGTGAACGGTATGTGAGGAGTGGGCAGTGGAAGAAGGGCGGCTACAAGTTGACCACCAAGTTCACCGGGAAAACTGTTGGGATCATCGGTCTCGGAAGAATTGGCAAAGCAGTTGCGAAGAGATCCGAGGCTTTTAGTTGCCCAATTGCTTACTTTTCCAGGACAGAAAAGCCAGATTCGAAGTTCAAATACTACCCAACTGTCGTGGAGTTGGCCGCCAACTGCCATGTTTTAGTTGTTGCATGCCCCTTAACCGAAGAGACCCGCCACATTATCAACCGCCAAGTCATTAATGCTTTGGGACCAAGGGGTGTTCTCATCAACATTGGGAGAGGTCCTCATGTTGACGAAGCTGAGTTGGTATCTGCTCTGGTAGAAGGCCGGTTAGGTGGGGCGGGGCTTGATGTCTATGAGAAGGAACCCCACGTACCCGAGCAGCTGTTCGCTCTCGAGAATGTGGTCCTCTTGCCTCATGTCGGAAGCGGCACGGTAGAAACTCGCAATGCCATGGCTGACCTTGTGGTTGGGAATCTTGAGGCTCACTTCTTGAAGAAACCATTGTTGACACCAGTGGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0008152 GO:0009853 GO:0009854 GO:0009987 GO:0016491 GO:0016614 GO:0016616 GO:0016618 GO:0030267 GO:0043094 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0055114

KEGG Pathways

Metabolic & Signaling

ko00130 ko00260 ko00350 ko00360 ko00630 ko00960 ko01100 ko01110 ko01130 ko01200 map00130 map00260 map00350 map00360 map00630 map00960 map01100 map01110 map01130 map01200

Pfam Domains

Protein Families

2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

376

Amino Acids

41.02

Weight (kDa)

9.02

Isoelectric Point (pI)

34.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
2-Hacid_dh	PF00389	97 - 374	2.2e-24	D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C	PF02826	171 - 343	1.5e-53	D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002512)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79870 AT1G79870
fragaria_vesca	FvH4_6g31711 FvH4_6g31720 FvH4_6g31721
malus_domestica	MD09G1209700.v1.1 MD09G1209800.v1.1 MD17G1191700.v1.1
prunus_persica	Prupe.3G067700_v2.0.a1 Prupe.3G067700_v2.0.a1 Prupe.3G067700_v2.0.a1 Prupe.3G067800_v2.0.a1
pyrus_communis	pycom09g12600 pycom09g12630 pycom17g19870
rosa_chinensis	RchiOBHm_Chr2g0138801 RchiOBHm_Chr2g0138891 RchiOBHm_Chr2g0138911
rosa_laevigata	RLG00000019761 RLG00000019763 RLG00000019764
rosa_multiflora	Rmu_co8474001.1_g000001 Rmu_sc0010783.1_g000009 Rmu_sc0011444.1_g000002 Rmu_sc0016387.1_g000002 Rmu_sc0017407.1_g000002
rosa_roxburghii	Rroxscaffold_2G00105920 Rroxscaffold_2G00105950
rosa_rugosa	Rorug02G0352600 Rorug02G0352800 Rorug02G0352900
rosa_samantha	Rh2AG402500 Rh2AG403000 Rh2BG411600 Rh2CG388600 Rh2DG422700 Rh2DG423100 Rh2DG423200
rosa_wichuraiana	Rw2G032720 Rw2G032780 Rw2G032790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	377
AccII	CGCG	1 cut(s)	327
AclWI	GGATC	3 cut(s)	208, 638, 666
AcoI	YGGCCR	1 cut(s)	762
AcyI	GRCGYC	2 cut(s)	201, 360
AfaI	GTAC	1 cut(s)	978
AfeI	AGCGCT	1 cut(s)	254
AfiI	CCNNNNNNNGG	1 cut(s)	1025
AflIII	ACRYGT	1 cut(s)	440
AgsI	TTSAA	5 cut(s)	13, 259, 436, 736, 1099
AjiI	CACGTC	2 cut(s)	441, 496
AjnI	CCWGG	2 cut(s)	272, 707
AluBI	AGCT	7 cut(s)	54, 76, 244, 367, 388, 902, 988
AluI	AGCT	7 cut(s)	54, 76, 244, 367, 388, 902, 988
Alw21I	GWGCWC	1 cut(s)	369
Alw26I	GTCTC	3 cut(s)	529, 644, 803
AlwI	GGATC	3 cut(s)	208, 638, 666
Ama87I	CYCGRG	2 cut(s)	980, 998
Aor51HI	AGCGCT	1 cut(s)	254
AoxI	GGCC	3 cut(s)	762, 928, 1122
ApeKI	GCWGC	1 cut(s)	985
AseI	ATTAAT	1 cut(s)	840
Asp700I	GAANNNNTTC	1 cut(s)	254
AspLEI	GCGC	2 cut(s)	255, 329
AspS9I	GGNCC	5 cut(s)	307, 502, 851, 884, 1009
AsuC2I	CCSGG	1 cut(s)	616
AsuHPI	GGTGA	2 cut(s)	457, 604
AsuII	TTCGAA	1 cut(s)	728
AvaI	CYCGRG	2 cut(s)	980, 998
AvaII	GGWCC	5 cut(s)	307, 502, 851, 884, 1009
BanII	GRGCYC	1 cut(s)	369
BarI	GAAGNNNNNNTAC	4 cut(s)	575, 607, 722, 754
BbsI	GAAGAC	1 cut(s)	443
Bbv12I	GWGCWC	1 cut(s)	369
BbvI	GCAGC	1 cut(s)	997
BccI	CCATC	5 cut(s)	69, 121, 203, 329, 338
BceAI	ACGGC	2 cut(s)	153, 554
BciT130I	CCWGG	2 cut(s)	274, 709
BcnI	CCSGG	1 cut(s)	616
BcoDI	GTCTC	3 cut(s)	529, 644, 803
BfoI	RGCGCY	1 cut(s)	256
BisI	GCNGC	5 cut(s)	375, 589, 765, 986, 1033
BlsI	GCNGC	5 cut(s)	376, 590, 766, 987, 1034
Bme1390I	CCNGG	3 cut(s)	274, 616, 709
Bme18I	GGWCC	5 cut(s)	307, 502, 851, 884, 1009
BmeT110I	CYCGRG	2 cut(s)	980, 998
BmgBI	CACGTC	2 cut(s)	441, 496
BmgT120I	GGNCC	5 cut(s)	307, 502, 851, 884, 1009
BmiI	GGNNCC	3 cut(s)	355, 852, 969
BmrFI	CCNGG	3 cut(s)	274, 616, 709
BmrI	ACTGGG	1 cut(s)	289
BmuI	ACTGGG	1 cut(s)	289
BpiI	GAAGAC	1 cut(s)	443
BplI	GAGNNNNNCTC	2 cut(s)	871, 903
BpmI	CTGGAG	1 cut(s)	410
Bpu14I	TTCGAA	1 cut(s)	728
BpuEI	CTTGAG	3 cut(s)	251, 266, 1103
BpuMI	CCSGG	1 cut(s)	616
Bsa29I	ATCGAT	1 cut(s)	420
BsaAI	YACGTR	1 cut(s)	976
BsaHI	GRCGYC	2 cut(s)	201, 360
BsaI	GGTCTC	2 cut(s)	644, 803
BsaJI	CCNNGG	4 cut(s)	272, 675, 854, 1056
BsaXI	ACNNNNNCTCC	2 cut(s)	302, 332
Bsc4I	CCNNNNNNNGG	1 cut(s)	1025
Bse118I	RCCGGY	1 cut(s)	930
Bse1I	ACTGG	3 cut(s)	295, 402, 1118
Bse3DI	GCAATG	1 cut(s)	1057
BseBI	CCWGG	2 cut(s)	274, 709
BseCI	ATCGAT	1 cut(s)	420
BseDI	CCNNGG	4 cut(s)	272, 675, 854, 1056
BseGI	GGATG	2 cut(s)	113, 321
BseLI	CCNNNNNNNGG	1 cut(s)	1025
BseMI	GCAATG	1 cut(s)	1057
BseMII	CTCAG	2 cut(s)	524, 894
BseNI	ACTGG	3 cut(s)	295, 402, 1118
BseRI	GAGGAG	2 cut(s)	254, 580
BseXI	GCAGC	1 cut(s)	997
Bsh1236I	CGCG	1 cut(s)	327
BshFI	GGCC	3 cut(s)	764, 930, 1124
BshVI	ATCGAT	1 cut(s)	420
BsiHKAI	GWGCWC	1 cut(s)	369
BsiHKCI	CYCGRG	2 cut(s)	980, 998
BsiSI	CCGG	3 cut(s)	351, 615, 931
BslFI	GGGAC	3 cut(s)	148, 266, 864
BslI	CCNNNNNNNGG	1 cut(s)	1025
BsmAI	GTCTC	3 cut(s)	529, 644, 803
BsmBI	CGTCTC	1 cut(s)	529
BsmFI	GGGAC	3 cut(s)	148, 266, 864
BsnI	GGCC	3 cut(s)	764, 930, 1124
Bso31I	GGTCTC	2 cut(s)	644, 803
BsoBI	CYCGRG	2 cut(s)	980, 998
Bsp119I	TTCGAA	1 cut(s)	728
Bsp1286I	GDGCHC	1 cut(s)	369
Bsp143I	GATC	3 cut(s)	213, 630, 671
Bsp19I	CCATGG	1 cut(s)	1056
BspANI	GGCC	3 cut(s)	764, 930, 1124
BspCNI	CTCAG	2 cut(s)	525, 895
BspDI	ATCGAT	1 cut(s)	420
BspFNI	CGCG	1 cut(s)	327
BspLI	GGNNCC	3 cut(s)	355, 852, 969
BspPI	GGATC	3 cut(s)	208, 638, 666
BspT104I	TTCGAA	1 cut(s)	728
BspTNI	GGTCTC	2 cut(s)	644, 803
BsrBI	CCGCTC	1 cut(s)	377
BsrDI	GCAATG	1 cut(s)	1057
BsrFI	RCCGGY	1 cut(s)	930
BsrI	ACTGG	3 cut(s)	295, 402, 1118
BssAI	RCCGGY	1 cut(s)	930
BssECI	CCNNGG	4 cut(s)	272, 675, 854, 1056
BssMI	GATC	3 cut(s)	213, 630, 671
BssNI	GRCGYC	2 cut(s)	201, 360
BssT1I	CCWWGG	2 cut(s)	854, 1056
Bst2UI	CCWGG	2 cut(s)	274, 709
Bst4CI	ACNGT	5 cut(s)	280, 558, 625, 751, 1039
Bst6I	CTCTTC	2 cut(s)	662, 801
BstACI	GRCGYC	2 cut(s)	201, 360
BstBAI	YACGTR	1 cut(s)	976
BstBI	TTCGAA	1 cut(s)	728
BstC8I	GCNNGC	1 cut(s)	793
BstDEI	CTNAG	2 cut(s)	533, 903
BstDSI	CCRYGG	1 cut(s)	1056
BstEII	GGTNACC	1 cut(s)	463
BstF5I	GGATG	2 cut(s)	113, 321
BstFNI	CGCG	1 cut(s)	327
BstH2I	RGCGCY	1 cut(s)	256
BstHHI	GCGC	2 cut(s)	255, 329
BstKTI	GATC	3 cut(s)	216, 633, 674
BstMAI	GTCTC	3 cut(s)	529, 644, 803
BstMBI	GATC	3 cut(s)	213, 630, 671
BstMWI	GCNNNNNNNGC	3 cut(s)	250, 506, 538
BstNI	CCWGG	2 cut(s)	274, 709
BstNSI	RCATGY	1 cut(s)	795
BstPI	GGTNACC	1 cut(s)	463
BstSCI	CCNGG	3 cut(s)	272, 614, 707
BstUI	CGCG	1 cut(s)	327
BstV1I	GCAGC	1 cut(s)	997
BstV2I	GAAGAC	1 cut(s)	443
BstX2I	RGATCY	1 cut(s)	671
BstXI	CCANNNNNNTGG	1 cut(s)	187
BstYI	RGATCY	1 cut(s)	671
Bsu15I	ATCGAT	1 cut(s)	420
BsuRI	GGCC	3 cut(s)	764, 930, 1124
BsuTUI	ATCGAT	1 cut(s)	420
BtgI	CCRYGG	1 cut(s)	1056
BtgZI	GCGATG	2 cut(s)	47, 355
BtrI	CACGTC	2 cut(s)	441, 496
BtsCI	GGATG	2 cut(s)	113, 321
BtsI	GCAGTG	1 cut(s)	581
BtsIMutI	CAGTG	2 cut(s)	581, 1125
Cac8I	GCNNGC	1 cut(s)	793
CfoI	GCGC	2 cut(s)	255, 329
Cfr10I	RCCGGY	1 cut(s)	930
Cfr13I	GGNCC	5 cut(s)	307, 502, 851, 884, 1009
ClaI	ATCGAT	1 cut(s)	420
CseI	GACGC	3 cut(s)	190, 368, 400
Csp6I	GTAC	1 cut(s)	977
CspCI	CAANNNNNGTGG	2 cut(s)	735, 770
CviAII	CATG	7 cut(s)	208, 220, 776, 792, 890, 1022, 1057
CviQI	GTAC	1 cut(s)	977
DdeI	CTNAG	2 cut(s)	533, 903
DpnI	GATC	3 cut(s)	215, 632, 673
DpnII	GATC	3 cut(s)	213, 630, 671
EaeI	YGGCCR	1 cut(s)	762
Eam1104I	CTCTTC	2 cut(s)	662, 801
EarI	CTCTTC	2 cut(s)	662, 801
EciI	GGCGGA	2 cut(s)	337, 515
Ecl136II	GAGCTC	1 cut(s)	367
Eco130I	CCWWGG	2 cut(s)	854, 1056
Eco24I	GRGCYC	1 cut(s)	369
Eco31I	GGTCTC	2 cut(s)	644, 803
Eco47I	GGWCC	5 cut(s)	307, 502, 851, 884, 1009
Eco47III	AGCGCT	1 cut(s)	254
Eco53kI	GAGCTC	1 cut(s)	367
Eco88I	CYCGRG	2 cut(s)	980, 998
Eco91I	GGTNACC	1 cut(s)	463
EcoICRI	GAGCTC	1 cut(s)	367
EcoO109I	RGGNCCY	1 cut(s)	884
EcoO65I	GGTNACC	1 cut(s)	463
EcoRII	CCWGG	2 cut(s)	272, 707
EcoT14I	CCWWGG	2 cut(s)	854, 1056
EcoT38I	GRGCYC	1 cut(s)	369
ErhI	CCWWGG	2 cut(s)	854, 1056
Esp3I	CGTCTC	1 cut(s)	529
FaeI	CATG	7 cut(s)	211, 223, 779, 795, 893, 1025, 1060
FaqI	GGGAC	3 cut(s)	148, 266, 864
FatI	CATG	7 cut(s)	207, 219, 775, 791, 889, 1021, 1056
FauI	CCCGC	2 cut(s)	821, 937
Fnu4HI	GCNGC	5 cut(s)	375, 589, 765, 986, 1033
FokI	GGATG	2 cut(s)	100, 308
FriOI	GRGCYC	1 cut(s)	369
Fsp4HI	GCNGC	5 cut(s)	375, 589, 765, 986, 1033
GlaI	GCGC	2 cut(s)	254, 328
GluI	GCNGC	5 cut(s)	375, 589, 765, 986, 1033
GsuI	CTGGAG	1 cut(s)	410
HaeII	RGCGCY	1 cut(s)	256
HaeIII	GGCC	3 cut(s)	764, 930, 1124
HapII	CCGG	3 cut(s)	351, 615, 931
HgaI	GACGC	3 cut(s)	190, 368, 400
HhaI	GCGC	2 cut(s)	255, 329
Hin1I	GRCGYC	2 cut(s)	201, 360
Hin1II	CATG	7 cut(s)	211, 223, 779, 795, 893, 1025, 1060
Hin6I	GCGC	2 cut(s)	253, 327
HinP1I	GCGC	2 cut(s)	253, 327
HincII	GTYRAC	3 cut(s)	600, 895, 1113
HindII	GTYRAC	3 cut(s)	600, 895, 1113
HindIII	AAGCTT	1 cut(s)	74
HinfI	GANTC	4 cut(s)	191, 417, 725, 1078
HpaII	CCGG	3 cut(s)	351, 615, 931
HphI	GGTGA	2 cut(s)	457, 604
Hpy166II	GTNNAC	5 cut(s)	554, 600, 612, 895, 1113
Hpy188I	TCNGA	5 cut(s)	49, 416, 644, 676, 1028
Hpy188III	TCNNGA	4 cut(s)	998, 1000, 1082, 1096
Hpy8I	GTNNAC	5 cut(s)	554, 600, 612, 895, 1113
Hpy99I	CGWCG	4 cut(s)	338, 362, 416, 482
HpyAV	CCTTC	4 cut(s)	111, 577, 920, 958
HpyCH4III	ACNGT	5 cut(s)	280, 558, 625, 751, 1039
HpyCH4IV	ACGT	5 cut(s)	440, 480, 495, 521, 975
HpyCH4V	TGCA	1 cut(s)	791
HpyF10VI	GCNNNNNNNGC	3 cut(s)	250, 506, 538
HpyF3I	CTNAG	2 cut(s)	533, 903
HpySE526I	ACGT	5 cut(s)	440, 480, 495, 521, 975
Hsp92I	GRCGYC	2 cut(s)	201, 360
Hsp92II	CATG	7 cut(s)	211, 223, 779, 795, 893, 1025, 1060
HspAI	GCGC	2 cut(s)	253, 327
Kzo9I	GATC	3 cut(s)	213, 630, 671
LmnI	GCTCC	4 cut(s)	59, 235, 241, 353
Lsp1109I	GCAGC	1 cut(s)	997
MaeII	ACGT	5 cut(s)	440, 480, 495, 521, 975
MaeIII	GTNAC	2 cut(s)	29, 463
MalI	GATC	3 cut(s)	215, 632, 673
MbiI	CCGCTC	1 cut(s)	377
MboI	GATC	3 cut(s)	213, 630, 671
MboII	GAAGA	6 cut(s)	448, 592, 657, 679, 818, 1111
MfeI	CAATTG	1 cut(s)	693
MflI	RGATCY	1 cut(s)	671
MhlI	GDGCHC	1 cut(s)	369
MluCI	AATT	2 cut(s)	648, 693
MlyI	GAGTC	1 cut(s)	200
MmeI	TCCRAC	2 cut(s)	394, 1006
MroXI	GAANNNNTTC	1 cut(s)	254
MseI	TTAA	2 cut(s)	800, 840
MslI	CAYNNNNRTG	1 cut(s)	185
MspA1I	CMGCKG	1 cut(s)	988
MspI	CCGG	3 cut(s)	351, 615, 931
MspR9I	CCNGG	3 cut(s)	274, 616, 709
MunI	CAATTG	1 cut(s)	693
MvaI	CCWGG	2 cut(s)	274, 709
MvnI	CGCG	1 cut(s)	327
MwoI	GCNNNNNNNGC	3 cut(s)	250, 506, 538
NciI	CCSGG	1 cut(s)	616
NcoI	CCATGG	1 cut(s)	1056
NdeII	GATC	3 cut(s)	213, 630, 671
NlaIII	CATG	7 cut(s)	211, 223, 779, 795, 893, 1025, 1060
NlaIV	GGNNCC	3 cut(s)	355, 852, 969
NmeAIII	GCCGAG	1 cut(s)	388
NmuCI	GTSAC	2 cut(s)	29, 463
NspI	RCATGY	1 cut(s)	795
NspV	TTCGAA	1 cut(s)	728
PaeI	GCATGC	1 cut(s)	795
PaeR7I	CTCGAG	1 cut(s)	998
PcsI	WCGNNNNNNNCGW	1 cut(s)	426
PdmI	GAANNNNTTC	1 cut(s)	254
PfeI	GAWTC	3 cut(s)	417, 725, 1078
PfoI	TCCNGGA	1 cut(s)	707
PkrI	GCNGC	5 cut(s)	376, 590, 766, 987, 1034
PleI	GAGTC	1 cut(s)	199
PpsI	GAGTC	1 cut(s)	199
Ppu21I	YACGTR	1 cut(s)	976
PpuMI	RGGWCCY	1 cut(s)	884
PshBI	ATTAAT	1 cut(s)	840
Psp124BI	GAGCTC	1 cut(s)	369
Psp5II	RGGWCCY	1 cut(s)	884
Psp6I	CCWGG	2 cut(s)	272, 707
PspEI	GGTNACC	1 cut(s)	463
PspGI	CCWGG	2 cut(s)	272, 707
PspN4I	GGNNCC	3 cut(s)	355, 852, 969
PspPI	GGNCC	5 cut(s)	307, 502, 851, 884, 1009
PspPPI	RGGWCCY	1 cut(s)	884
PsrI	GAACNNNNNNTAC	2 cut(s)	960, 992
PsuI	RGATCY	1 cut(s)	671
PvuII	CAGCTG	1 cut(s)	988
RsaI	GTAC	1 cut(s)	978
RsaNI	GTAC	1 cut(s)	977
RseI	CAYNNNNRTG	1 cut(s)	185
SacI	GAGCTC	1 cut(s)	369
SaqAI	TTAA	2 cut(s)	800, 840
SatI	GCNGC	5 cut(s)	375, 589, 765, 986, 1033
Sau3AI	GATC	3 cut(s)	213, 630, 671
Sau96I	GGNCC	5 cut(s)	307, 502, 851, 884, 1009
SchI	GAGTC	1 cut(s)	200
ScrFI	CCNGG	3 cut(s)	274, 616, 709
SduI	GDGCHC	1 cut(s)	369
Sfr274I	CTCGAG	1 cut(s)	998
SfuI	TTCGAA	1 cut(s)	728
SinI	GGWCC	5 cut(s)	307, 502, 851, 884, 1009
SlaI	CTCGAG	1 cut(s)	998
SmiMI	CAYNNNNRTG	1 cut(s)	185
SmlI	CTYRAG	4 cut(s)	245, 266, 998, 1082
SmoI	CTYRAG	4 cut(s)	245, 266, 998, 1082
SphI	GCATGC	1 cut(s)	795
Sse9I	AATT	2 cut(s)	648, 693
SstI	GAGCTC	1 cut(s)	369
StyD4I	CCNGG	3 cut(s)	272, 614, 707
StyI	CCWWGG	2 cut(s)	854, 1056
TaaI	ACNGT	5 cut(s)	280, 558, 625, 751, 1039
TaiI	ACGT	5 cut(s)	443, 483, 498, 524, 978
TaqI	TCGA	4 cut(s)	420, 429, 728, 999
TaqII	GACCGA	1 cut(s)	626
TasI	AATT	2 cut(s)	648, 693
TauI	GCSGC	4 cut(s)	377, 591, 767, 1035
TfiI	GAWTC	3 cut(s)	417, 725, 1078
Tru1I	TTAA	2 cut(s)	800, 840
Tru9I	TTAA	2 cut(s)	800, 840
TscAI	CASTG	2 cut(s)	581, 1125
TseFI	GTSAC	2 cut(s)	29, 463
TseI	GCWGC	1 cut(s)	985
Tsp45I	GTSAC	2 cut(s)	29, 463
TspDTI	ATGAA	1 cut(s)	289
TspRI	CASTG	2 cut(s)	581, 1125
VpaK11BI	GGWCC	5 cut(s)	307, 502, 851, 884, 1009
VspI	ATTAAT	1 cut(s)	840
XceI	RCATGY	1 cut(s)	795
XhoI	CTCGAG	1 cut(s)	998
XmnI	GAANNNNTTC	1 cut(s)	254

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.