MD09G1214500.v1.1

Thionin-like protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004296
Physical Location & Seq
Reverse (-)
21087927 .. 21088990
1064 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1214500.v1.1.491

Sequence Viewer

Length: 360 bp
ATGGATAAGAAGGGATTGAGGCCAGTACCGGTGGCTTATTTAGTGTTGGGGATGTTGATGCTGGCGGGGCAGTCCAGTGCTTCTTTCAAAAGCTGCTACGAGAGTTGCTTCATCCTCTGTGTCATCACCCCAAACCACTCCGTTAGCTACTGCAGTTTTCAGTGCTTGAAAGATTGCATCATACCAAGTAGGTCTCCTTTTGATCATAGTAATATCCACAAAGACAATGTCTACTTCTGCAAACTTGGATGTGCTTCTTCCTTGTGTTCCAATCTCAGCACTAAACAAAACCCAGAGGTTGAGAAGGTAGGCGGCTGCGTGGATTCTTGCTCAAAGACATGTACCATCAAGAATACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

120

Amino Acids

12.97

Weight (kDa)

8.45

Isoelectric Point (pI)

54.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 231
AciI CCGC 2 cut(s) 65, 312
AfaI GTAC 2 cut(s) 27, 343
AflIII ACRYGT 1 cut(s) 338
AgeI ACCGGT 1 cut(s) 28
AgsI TTSAA 2 cut(s) 88, 169
AluBI AGCT 2 cut(s) 93, 147
AluI AGCT 2 cut(s) 93, 147
Alw26I GTCTC 1 cut(s) 198
AoxI GGCC 1 cut(s) 20
ApeKI GCWGC 2 cut(s) 93, 315
ArsI GACNNNNNNTTYG 2 cut(s) 213, 245
AsiGI ACCGGT 1 cut(s) 28
AsuHPI GGTGA 1 cut(s) 118
BbvI GCAGC 2 cut(s) 80, 302
BccI CCATC 1 cut(s) 353
BclI TGATCA 1 cut(s) 202
BcoDI GTCTC 1 cut(s) 198
BfmI CTRYAG 1 cut(s) 151
BisI GCNGC 3 cut(s) 94, 313, 316
BlsI GCNGC 3 cut(s) 95, 314, 317
BmsI GCATC 2 cut(s) 48, 186
BsaI GGTCTC 1 cut(s) 198
BsaWI WCCGGW 1 cut(s) 28
Bse118I RCCGGY 1 cut(s) 28
Bse1I ACTGG 2 cut(s) 23, 75
BseGI GGATG 3 cut(s) 57, 111, 254
BseMII CTCAG 1 cut(s) 289
BseNI ACTGG 2 cut(s) 23, 75
BseXI GCAGC 2 cut(s) 80, 302
BshFI GGCC 1 cut(s) 22
BshTI ACCGGT 1 cut(s) 28
BsiSI CCGG 1 cut(s) 29
BsmAI GTCTC 1 cut(s) 198
BsnI GGCC 1 cut(s) 22
Bso31I GGTCTC 1 cut(s) 198
Bsp143I GATC 1 cut(s) 202
BspACI CCGC 2 cut(s) 65, 312
BspANI GGCC 1 cut(s) 22
BspCNI CTCAG 1 cut(s) 288
BspMAI CTGCAG 1 cut(s) 155
BspTNI GGTCTC 1 cut(s) 198
BsrFI RCCGGY 1 cut(s) 28
BsrI ACTGG 2 cut(s) 23, 75
BssAI RCCGGY 1 cut(s) 28
BssMI GATC 1 cut(s) 202
BstC8I GCNNGC 1 cut(s) 63
BstDEI CTNAG 1 cut(s) 275
BstF5I GGATG 3 cut(s) 57, 111, 254
BstKTI GATC 1 cut(s) 205
BstMAI GTCTC 1 cut(s) 198
BstMBI GATC 1 cut(s) 202
BstMWI GCNNNNNNNGC 1 cut(s) 67
BstNSI RCATGY 1 cut(s) 342
BstSFI CTRYAG 1 cut(s) 151
BstV1I GCAGC 2 cut(s) 80, 302
BsuRI GGCC 1 cut(s) 22
BtsCI GGATG 3 cut(s) 57, 111, 254
BtsIMutI CAGTG 2 cut(s) 82, 167
Cac8I GCNNGC 1 cut(s) 63
Cfr10I RCCGGY 1 cut(s) 28
Csp6I GTAC 2 cut(s) 26, 342
CspAI ACCGGT 1 cut(s) 28
CviAII CATG 2 cut(s) 339, 357
CviJI RGCY 5 cut(s) 22, 35, 93, 147, 315
CviKI_1 RGCY 5 cut(s) 22, 35, 93, 147, 315
CviQI GTAC 2 cut(s) 26, 342
DdeI CTNAG 1 cut(s) 275
DpnI GATC 1 cut(s) 204
DpnII GATC 1 cut(s) 202
Eco31I GGTCTC 1 cut(s) 198
FaeI CATG 2 cut(s) 342, 360
FaiI YATR 4 cut(s) 182, 207, 340, 358
FatI CATG 2 cut(s) 338, 356
FauI CCCGC 1 cut(s) 58
FbaI TGATCA 1 cut(s) 202
FblI GTMKAC 1 cut(s) 231
Fnu4HI GCNGC 3 cut(s) 94, 313, 316
FokI GGATG 3 cut(s) 64, 98, 261
Fsp4HI GCNGC 3 cut(s) 94, 313, 316
GluI GCNGC 3 cut(s) 94, 313, 316
HaeIII GGCC 1 cut(s) 22
HapII CCGG 1 cut(s) 29
Hin1II CATG 2 cut(s) 342, 360
HinfI GANTC 1 cut(s) 323
HpaII CCGG 1 cut(s) 29
HphI GGTGA 1 cut(s) 118
Hpy166II GTNNAC 1 cut(s) 232
Hpy188III TCNNGA 1 cut(s) 349
Hpy8I GTNNAC 1 cut(s) 232
HpyAV CCTTC 2 cut(s) 4, 298
HpyCH4V TGCA 3 cut(s) 153, 177, 240
HpyF10VI GCNNNNNNNGC 1 cut(s) 67
HpyF3I CTNAG 1 cut(s) 275
Hsp92II CATG 2 cut(s) 342, 360
Ksp22I TGATCA 1 cut(s) 202
Kzo9I GATC 1 cut(s) 202
LpnPI CCDG 5 cut(s) 36, 42, 47, 88, 306
Lsp1109I GCAGC 2 cut(s) 80, 302
LweI GCATC 2 cut(s) 48, 186
MalI GATC 1 cut(s) 204
MboI GATC 1 cut(s) 202
MboII GAAGA 1 cut(s) 249
MnlI CCTC 3 cut(s) 12, 125, 289
MspI CCGG 1 cut(s) 29
MwoI GCNNNNNNNGC 1 cut(s) 67
NdeII GATC 1 cut(s) 202
NlaIII CATG 2 cut(s) 342, 360
NspI RCATGY 1 cut(s) 342
PciI ACATGT 1 cut(s) 338
PfeI GAWTC 1 cut(s) 323
PflFI GACNNNGTC 1 cut(s) 227
PinAI ACCGGT 1 cut(s) 28
PkrI GCNGC 3 cut(s) 95, 314, 317
PscI ACATGT 1 cut(s) 338
PstI CTGCAG 1 cut(s) 155
PsyI GACNNNGTC 1 cut(s) 227
RsaI GTAC 2 cut(s) 27, 343
RsaNI GTAC 2 cut(s) 26, 342
SatI GCNGC 3 cut(s) 94, 313, 316
Sau3AI GATC 1 cut(s) 202
SetI ASST 5 cut(s) 95, 149, 194, 300, 309
SfaNI GCATC 2 cut(s) 48, 186
SfcI CTRYAG 1 cut(s) 151
SsiI CCGC 2 cut(s) 65, 312
TauI GCSGC 1 cut(s) 315
TfiI GAWTC 1 cut(s) 323
TscAI CASTG 2 cut(s) 82, 167
TseI GCWGC 2 cut(s) 93, 315
TspDTI ATGAA 1 cut(s) 100
TspGWI ACGGA 1 cut(s) 130
TspRI CASTG 2 cut(s) 82, 167
Tth111I GACNNNGTC 1 cut(s) 227
XceI RCATGY 1 cut(s) 342
XmiI GTMKAC 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.