Rw2G032050

Thionin-like protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0004296
Physical Location & Seq
Forward (+)
52832840 .. 52833307
468 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G032050.1

Sequence Viewer

Length: 345 bp
ATGGATACCAAGGAAGTCAGGTCCTTCGCCATCATCACTCACCTAGTGGTATTTGGGATGTTATTGGTTCAGTCCACTGCTTCTTTCAAAAGCTGCTACGAGAGCTGCTTCCTTCTCTGTGTCATCACTCCCCACCAATCTGTTGGCTCATGCTCCTTCAAATGCTTGAAAGACTGCATCATACCGACGTCTTATAGTACCAACACAGATAACCAATACTTTTGCGCGCTCGGTTGTGCTTCTTCATTGTGTTCCAGTTTCAGTACCAAGCAAAACCCAGAGTTGGAGAAAGTGGGTGGCTGCGTAGATTCATGCTCAGAGACCTGTAGTACCAAGAACACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

114

Amino Acids

12.36

Weight (kDa)

6.03

Isoelectric Point (pI)

32.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 191
AccII CGCG 1 cut(s) 227
AcyI GRCGYC 1 cut(s) 188
AdeI CACNNNGTG 1 cut(s) 46
AfaI GTAC 3 cut(s) 199, 265, 331
AfiI CCNNNNNNNGG 1 cut(s) 283
AgsI TTSAA 3 cut(s) 88, 160, 169
AluBI AGCT 2 cut(s) 93, 105
AluI AGCT 2 cut(s) 93, 105
Alw26I GTCTC 1 cut(s) 314
ApeKI GCWGC 3 cut(s) 93, 105, 300
AspLEI GCGC 2 cut(s) 227, 229
AspS9I GGNCC 1 cut(s) 21
AsuHPI GGTGA 1 cut(s) 32
AvaII GGWCC 1 cut(s) 21
BbvI GCAGC 3 cut(s) 80, 92, 287
BccI CCATC 1 cut(s) 38
BcoDI GTCTC 1 cut(s) 314
BfaI CTAG 1 cut(s) 44
BfmI CTRYAG 1 cut(s) 325
BisI GCNGC 3 cut(s) 94, 106, 301
BlsI GCNGC 3 cut(s) 95, 107, 302
Bme18I GGWCC 1 cut(s) 21
BmgT120I GGNCC 1 cut(s) 21
BmsI GCATC 1 cut(s) 186
BsaHI GRCGYC 1 cut(s) 188
BsaI GGTCTC 1 cut(s) 314
BsaJI CCNNGG 1 cut(s) 9
Bsc4I CCNNNNNNNGG 1 cut(s) 283
Bse1I ACTGG 1 cut(s) 255
BseDI CCNNGG 1 cut(s) 9
BseGI GGATG 1 cut(s) 63
BseLI CCNNNNNNNGG 1 cut(s) 283
BseMII CTCAG 1 cut(s) 330
BseNI ACTGG 1 cut(s) 255
BsePI GCGCGC 1 cut(s) 225
BseXI GCAGC 3 cut(s) 80, 92, 287
Bsh1236I CGCG 1 cut(s) 227
BslI CCNNNNNNNGG 1 cut(s) 283
BsmAI GTCTC 1 cut(s) 314
Bso31I GGTCTC 1 cut(s) 314
BspCNI CTCAG 1 cut(s) 329
BspFNI CGCG 1 cut(s) 227
BspTNI GGTCTC 1 cut(s) 314
BsrI ACTGG 1 cut(s) 255
BssECI CCNNGG 1 cut(s) 9
BssHII GCGCGC 1 cut(s) 225
BssNI GRCGYC 1 cut(s) 188
BssT1I CCWWGG 1 cut(s) 9
BstACI GRCGYC 1 cut(s) 188
BstC8I GCNNGC 1 cut(s) 227
BstDEI CTNAG 1 cut(s) 316
BstF5I GGATG 1 cut(s) 63
BstFNI CGCG 1 cut(s) 227
BstHHI GCGC 2 cut(s) 227, 229
BstMAI GTCTC 1 cut(s) 314
BstMWI GCNNNNNNNGC 1 cut(s) 102
BstSFI CTRYAG 1 cut(s) 325
BstUI CGCG 1 cut(s) 227
BstV1I GCAGC 3 cut(s) 80, 92, 287
BstXI CCANNNNNNTGG 1 cut(s) 143
BtsCI GGATG 1 cut(s) 63
BtsI GCAGTG 1 cut(s) 75
BtsIMutI CAGTG 1 cut(s) 75
Cac8I GCNNGC 1 cut(s) 227
CfoI GCGC 2 cut(s) 227, 229
Cfr13I GGNCC 1 cut(s) 21
Csp6I GTAC 3 cut(s) 198, 264, 330
CviAII CATG 2 cut(s) 150, 312
CviJI RGCY 4 cut(s) 93, 105, 147, 300
CviKI_1 RGCY 4 cut(s) 93, 105, 147, 300
CviQI GTAC 3 cut(s) 198, 264, 330
DdeI CTNAG 1 cut(s) 316
DraIII CACNNNGTG 1 cut(s) 46
Eco130I CCWWGG 1 cut(s) 9
Eco31I GGTCTC 1 cut(s) 314
Eco47I GGWCC 1 cut(s) 21
EcoO109I RGGNCCY 1 cut(s) 21
EcoT14I CCWWGG 1 cut(s) 9
ErhI CCWWGG 1 cut(s) 9
FaeI CATG 2 cut(s) 153, 315
FaiI YATR 4 cut(s) 151, 182, 195, 313
FatI CATG 2 cut(s) 149, 311
Fnu4HI GCNGC 3 cut(s) 94, 106, 301
FokI GGATG 1 cut(s) 70
Fsp4HI GCNGC 3 cut(s) 94, 106, 301
FspBI CTAG 1 cut(s) 44
GlaI GCGC 2 cut(s) 226, 228
GluI GCNGC 3 cut(s) 94, 106, 301
HhaI GCGC 2 cut(s) 227, 229
Hin1I GRCGYC 1 cut(s) 188
Hin1II CATG 2 cut(s) 153, 315
Hin6I GCGC 2 cut(s) 225, 227
HinP1I GCGC 2 cut(s) 225, 227
HinfI GANTC 1 cut(s) 308
HphI GGTGA 1 cut(s) 32
Hpy166II GTNNAC 1 cut(s) 75
Hpy188I TCNGA 1 cut(s) 319
Hpy8I GTNNAC 1 cut(s) 75
Hpy99I CGWCG 1 cut(s) 190
HpyAV CCTTC 3 cut(s) 34, 122, 166
HpyCH4IV ACGT 1 cut(s) 188
HpyCH4V TGCA 1 cut(s) 177
HpyF10VI GCNNNNNNNGC 1 cut(s) 102
HpyF3I CTNAG 1 cut(s) 316
HpySE526I ACGT 1 cut(s) 188
Hsp92I GRCGYC 1 cut(s) 188
Hsp92II CATG 2 cut(s) 153, 315
HspAI GCGC 2 cut(s) 225, 227
LmnI GCTCC 1 cut(s) 158
LpnPI CCDG 4 cut(s) 4, 268, 291, 337
Lsp1109I GCAGC 3 cut(s) 80, 92, 287
LweI GCATC 1 cut(s) 186
MaeI CTAG 1 cut(s) 44
MaeII ACGT 1 cut(s) 188
MboII GAAGA 1 cut(s) 234
MmeI TCCRAC 1 cut(s) 264
MvnI CGCG 1 cut(s) 227
MwoI GCNNNNNNNGC 1 cut(s) 102
NlaIII CATG 2 cut(s) 153, 315
PauI GCGCGC 1 cut(s) 225
PfeI GAWTC 1 cut(s) 308
PkrI GCNGC 3 cut(s) 95, 107, 302
PpuMI RGGWCCY 1 cut(s) 21
Psp5II RGGWCCY 1 cut(s) 21
PspPI GGNCC 1 cut(s) 21
PspPPI RGGWCCY 1 cut(s) 21
PteI GCGCGC 1 cut(s) 225
RsaI GTAC 3 cut(s) 199, 265, 331
RsaNI GTAC 3 cut(s) 198, 264, 330
SatI GCNGC 3 cut(s) 94, 106, 301
Sau96I GGNCC 1 cut(s) 21
SetI ASST 7 cut(s) 23, 45, 95, 107, 191, 326, 344
SfaNI GCATC 1 cut(s) 186
SfcI CTRYAG 1 cut(s) 325
SinI GGWCC 1 cut(s) 21
SspMI CTAG 1 cut(s) 44
StyI CCWWGG 1 cut(s) 9
TaiI ACGT 1 cut(s) 191
TfiI GAWTC 1 cut(s) 308
TscAI CASTG 1 cut(s) 82
TseI GCWGC 3 cut(s) 93, 105, 300
TspDTI ATGAA 2 cut(s) 234, 300
TspRI CASTG 1 cut(s) 82
VpaK11BI GGWCC 1 cut(s) 21
XcmI CCANNNNNNNNNTGG 1 cut(s) 140
XspI CTAG 1 cut(s) 44
ZraI GACGTC 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.