MD09G1233000.v1.1

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0010583
Physical Location & Seq
Forward (+)
29212030 .. 29213280
1251 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1233000.v1.1.491

Sequence Viewer

Length: 708 bp
ATGAATAATAACCAAGCAAGTCAATCTTCTTCAACCAAAGTTGAAAGGAGGGTTGTAGAGAAGAATAGGAGAAATCATATGAAACTTCTTTACTCAAACCTCTTCTCTCTCCTCCCTAACCAAAATCCTAAGCCACTAAGTCTGCCCGAACAAATAGATGAGGCCATCAACTACATAAAAAGCAGAGAGTCGAAGCTGCAGAAATCAAAGGAGAAGAAGGAGAGTTTGGTGGGCACTAGAAAAAGATCACATGCTACGTCATTCGTAAATGTTGAGAGCATGGAACCAAACAACTTAAGTATTGAGAGCGTAAGGAGCACAAAAGCACCCCAAATCCAAATCCATGAGATAGGCTCCACAGTAGAGGTAGTACTGACTAGTGGCTTAGACAATCATCAGTTCATCTTCTACGAAATTATTCGAATACTTGAAGAAGAACAGGCGGACGTTGTGCATGCTAGCTTTTCAACTCTAGGAGACACTATATTTCATGTAGTACGTGCAGAGATGCACAAGTCTGTGTTGGATTTTGGGGCTGCAAGAATAACTGAAAAGCTTAATAGATTTGTCAATGGATCCAAAAGCGACCAAGAATTACAGCAAGATGGCTTTTGGGACTTCGAATTCGAGCCTGTGGCAATAATATGGGATCAGTTGACAAGTACTCCACGAAACAAATTAATCAATCCTATTTCCCCGGTGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

236

Amino Acids

26.85

Weight (kDa)

6.67

Isoelectric Point (pI)

52.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 15 - 61 5.2e-07 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 443
AclWI GGATC 3 cut(s) 570, 583, 657
AcsI RAATTY 1 cut(s) 623
AfaI GTAC 3 cut(s) 372, 498, 664
AflII CTTAAG 1 cut(s) 295
AgsI TTSAA 4 cut(s) 33, 44, 431, 468
AhlI ACTAGT 1 cut(s) 377
AjuI GAANNNNNNNTTGG 2 cut(s) 209, 241
AluBI AGCT 3 cut(s) 196, 462, 556
AluI AGCT 3 cut(s) 196, 462, 556
Alw21I GWGCWC 1 cut(s) 320
Alw26I GTCTC 1 cut(s) 471
AlwI GGATC 3 cut(s) 570, 583, 657
AoxI GGCC 1 cut(s) 162
ApeKI GCWGC 2 cut(s) 196, 536
ApoI RAATTY 1 cut(s) 623
ArsI GACNNNNNNTTYG 2 cut(s) 608, 640
AseI ATTAAT 1 cut(s) 680
Asp700I GAANNNNTTC 1 cut(s) 417
AsuC2I CCSGG 1 cut(s) 698
AsuII TTCGAA 2 cut(s) 421, 621
AsuNHI GCTAGC 1 cut(s) 458
BaeGI GKGCMC 1 cut(s) 236
BamHI GGATCC 1 cut(s) 575
Bbv12I GWGCWC 1 cut(s) 320
BbvI GCAGC 2 cut(s) 183, 523
BccI CCATC 2 cut(s) 173, 599
BcnI CCSGG 1 cut(s) 698
BcoDI GTCTC 1 cut(s) 471
BcuI ACTAGT 1 cut(s) 377
BfaI CTAG 4 cut(s) 237, 378, 459, 473
BfmI CTRYAG 1 cut(s) 197
BfrI CTTAAG 1 cut(s) 295
BisI GCNGC 2 cut(s) 197, 537
BlsI GCNGC 2 cut(s) 198, 538
BmcAI AGTACT 2 cut(s) 372, 664
Bme1390I CCNGG 1 cut(s) 698
BmiI GGNNCC 3 cut(s) 285, 355, 577
BmrFI CCNGG 1 cut(s) 698
BmsI GCATC 1 cut(s) 498
BmtI GCTAGC 1 cut(s) 462
BplI GAGNNNNNCTC 2 cut(s) 338, 370
Bpu10I CCTNAGC 1 cut(s) 129
Bpu14I TTCGAA 2 cut(s) 421, 621
BpuMI CCSGG 1 cut(s) 698
BsaAI YACGTR 1 cut(s) 500
BsaJI CCNNGG 1 cut(s) 696
BsaXI ACNNNNNCTCC 2 cut(s) 649, 679
BseDI CCNNGG 1 cut(s) 696
BseRI GAGGAG 1 cut(s) 101
BseSI GKGCMC 1 cut(s) 236
BseXI GCAGC 2 cut(s) 183, 523
BsgI GTGCAG 1 cut(s) 522
BshFI GGCC 1 cut(s) 164
BsiHKAI GWGCWC 1 cut(s) 320
BsiSI CCGG 1 cut(s) 698
BslFI GGGAC 1 cut(s) 629
BsmAI GTCTC 1 cut(s) 471
BsmFI GGGAC 1 cut(s) 629
BsnI GGCC 1 cut(s) 164
Bsp119I TTCGAA 2 cut(s) 421, 621
Bsp1286I GDGCHC 2 cut(s) 236, 320
Bsp143I GATC 3 cut(s) 245, 575, 649
BspACI CCGC 1 cut(s) 443
BspANI GGCC 1 cut(s) 164
BspLI GGNNCC 3 cut(s) 285, 355, 577
BspMAI CTGCAG 1 cut(s) 201
BspOI GCTAGC 1 cut(s) 462
BspPI GGATC 3 cut(s) 570, 583, 657
BspT104I TTCGAA 2 cut(s) 421, 621
BspTI CTTAAG 1 cut(s) 295
BssECI CCNNGG 1 cut(s) 696
BssMI GATC 3 cut(s) 245, 575, 649
Bst4CI ACNGT 1 cut(s) 361
Bst6I CTCTTC 1 cut(s) 107
BstAFI CTTAAG 1 cut(s) 295
BstBAI YACGTR 1 cut(s) 500
BstBI TTCGAA 2 cut(s) 421, 621
BstC8I GCNNGC 2 cut(s) 456, 460
BstDEI CTNAG 3 cut(s) 129, 137, 385
BstKTI GATC 3 cut(s) 248, 578, 652
BstMAI GTCTC 1 cut(s) 471
BstMBI GATC 3 cut(s) 245, 575, 649
BstMWI GCNNNNNNNGC 1 cut(s) 315
BstNSI RCATGY 2 cut(s) 254, 458
BstSCI CCNGG 1 cut(s) 696
BstSFI CTRYAG 1 cut(s) 197
BstSLI GKGCMC 1 cut(s) 236
BstV1I GCAGC 2 cut(s) 183, 523
BstX2I RGATCY 1 cut(s) 575
BstYI RGATCY 1 cut(s) 575
BsuRI GGCC 1 cut(s) 164
Cac8I GCNNGC 2 cut(s) 456, 460
Csp6I GTAC 3 cut(s) 371, 497, 663
CviAII CATG 5 cut(s) 251, 280, 344, 455, 491
CviQI GTAC 3 cut(s) 371, 497, 663
DdeI CTNAG 3 cut(s) 129, 137, 385
DpnI GATC 3 cut(s) 247, 577, 651
DpnII GATC 3 cut(s) 245, 575, 649
Eam1104I CTCTTC 1 cut(s) 107
EarI CTCTTC 1 cut(s) 107
EciI GGCGGA 1 cut(s) 458
EcoRI GAATTC 1 cut(s) 623
FaeI CATG 5 cut(s) 254, 283, 347, 458, 494
FalI AAGNNNNNCTT 2 cut(s) 10, 42
FaqI GGGAC 1 cut(s) 629
FatI CATG 5 cut(s) 250, 279, 343, 454, 490
FauNDI CATATG 1 cut(s) 78
Fnu4HI GCNGC 2 cut(s) 197, 537
Fsp4HI GCNGC 2 cut(s) 197, 537
FspBI CTAG 4 cut(s) 237, 378, 459, 473
GluI GCNGC 2 cut(s) 197, 537
HaeIII GGCC 1 cut(s) 164
HapII CCGG 1 cut(s) 698
Hin1II CATG 5 cut(s) 254, 283, 347, 458, 494
HincII GTYRAC 1 cut(s) 657
HindII GTYRAC 1 cut(s) 657
HindIII AAGCTT 1 cut(s) 554
HinfI GANTC 1 cut(s) 188
HpaII CCGG 1 cut(s) 698
Hpy166II GTNNAC 2 cut(s) 657, 703
Hpy8I GTNNAC 2 cut(s) 657, 703
HpyAV CCTTC 1 cut(s) 211
HpyCH4III ACNGT 1 cut(s) 361
HpyCH4IV ACGT 3 cut(s) 257, 447, 499
HpyCH4V TGCA 5 cut(s) 199, 454, 503, 511, 539
HpyF10VI GCNNNNNNNGC 1 cut(s) 315
HpyF3I CTNAG 3 cut(s) 129, 137, 385
HpySE526I ACGT 3 cut(s) 257, 447, 499
Hsp92II CATG 5 cut(s) 254, 283, 347, 458, 494
Kzo9I GATC 3 cut(s) 245, 575, 649
LmnI GCTCC 2 cut(s) 315, 359
LpnPI CCDG 2 cut(s) 425, 645
Lsp1109I GCAGC 2 cut(s) 183, 523
LweI GCATC 1 cut(s) 498
MaeI CTAG 4 cut(s) 237, 378, 459, 473
MaeII ACGT 3 cut(s) 257, 447, 499
MalI GATC 3 cut(s) 247, 577, 651
MboI GATC 3 cut(s) 245, 575, 649
MboII GAAGA 8 cut(s) 18, 21, 73, 94, 226, 397, 443, 446
MflI RGATCY 1 cut(s) 575
MhlI GDGCHC 2 cut(s) 236, 320
MluCI AATT 4 cut(s) 414, 593, 623, 677
MlyI GAGTC 1 cut(s) 197
MmeI TCCRAC 1 cut(s) 504
MnlI CCTC 5 cut(s) 42, 110, 122, 154, 358
MroXI GAANNNNTTC 1 cut(s) 417
MseI TTAA 3 cut(s) 296, 558, 680
MspCI CTTAAG 1 cut(s) 295
MspI CCGG 1 cut(s) 698
MspR9I CCNGG 1 cut(s) 698
MwoI GCNNNNNNNGC 1 cut(s) 315
NciI CCSGG 1 cut(s) 698
NdeI CATATG 1 cut(s) 78
NdeII GATC 3 cut(s) 245, 575, 649
NheI GCTAGC 1 cut(s) 458
NlaIII CATG 5 cut(s) 254, 283, 347, 458, 494
NlaIV GGNNCC 3 cut(s) 285, 355, 577
NspI RCATGY 2 cut(s) 254, 458
NspV TTCGAA 2 cut(s) 421, 621
PaeI GCATGC 1 cut(s) 458
PdmI GAANNNNTTC 1 cut(s) 417
PkrI GCNGC 2 cut(s) 198, 538
PleI GAGTC 1 cut(s) 196
PpsI GAGTC 1 cut(s) 196
Ppu21I YACGTR 1 cut(s) 500
PshBI ATTAAT 1 cut(s) 680
PspN4I GGNNCC 3 cut(s) 285, 355, 577
PstI CTGCAG 1 cut(s) 201
PsuI RGATCY 1 cut(s) 575
RsaI GTAC 3 cut(s) 372, 498, 664
RsaNI GTAC 3 cut(s) 371, 497, 663
SaqAI TTAA 3 cut(s) 296, 558, 680
SatI GCNGC 2 cut(s) 197, 537
Sau3AI GATC 3 cut(s) 245, 575, 649
ScaI AGTACT 2 cut(s) 372, 664
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 1 cut(s) 698
SduI GDGCHC 2 cut(s) 236, 320
SetI ASST 8 cut(s) 102, 198, 260, 369, 450, 464, 502, 558
SfaNI GCATC 1 cut(s) 498
SfcI CTRYAG 1 cut(s) 197
SfuI TTCGAA 2 cut(s) 421, 621
SmlI CTYRAG 1 cut(s) 295
SmoI CTYRAG 1 cut(s) 295
SpeI ACTAGT 1 cut(s) 377
SphI GCATGC 1 cut(s) 458
Sse9I AATT 4 cut(s) 414, 593, 623, 677
SsiI CCGC 1 cut(s) 443
SspMI CTAG 4 cut(s) 237, 378, 459, 473
StyD4I CCNGG 1 cut(s) 696
TaaI ACNGT 1 cut(s) 361
TaiI ACGT 3 cut(s) 260, 450, 502
TaqI TCGA 4 cut(s) 191, 421, 621, 627
TasI AATT 4 cut(s) 414, 593, 623, 677
TatI WGTACW 2 cut(s) 370, 662
Tru1I TTAA 3 cut(s) 296, 558, 680
Tru9I TTAA 3 cut(s) 296, 558, 680
TseI GCWGC 2 cut(s) 196, 536
TspDTI ATGAA 4 cut(s) 17, 95, 391, 479
Vha464I CTTAAG 1 cut(s) 295
VspI ATTAAT 1 cut(s) 680
XapI RAATTY 1 cut(s) 623
XceI RCATGY 2 cut(s) 254, 458
XmnI GAANNNNTTC 1 cut(s) 417
XspI CTAG 4 cut(s) 237, 378, 459, 473
ZrmI AGTACT 2 cut(s) 372, 664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.