MD17G1238500.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0010583
Physical Location & Seq
Reverse (-)
28766483 .. 28767103
621 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1238500.v1.1.491

Sequence Viewer

Length: 498 bp
ATGGATCGTAATCATGCAAATGAATCTTCTTCAACCAAAGTTGAAAGGAGGGTTGTTGAGAAGAACAGGAGAAATCGTATGAAACTTCTTTACTCCAACCTCTACTCCCTCCTCCCTAAACAAACTTTTAAGGAGCCACTAAGTCAGACAGATCAAATAGATGAAGCCATAAACTACATAAAAAGCCAAGAGTCGAAGCTGCAGAAGCTAAAGGAGAAGAAGGAGAGCTTAATGCGCACTAGAAAAAGATCATATGCTACAGCATGCGTAAATGTTGAGAGCACAAGGAGCGCAAAGGCACCCCACATCAAAATCCATGAGAAAGACTCCACACTAGAGGTAGTACTGATAAGTGGCTTAGAAAATCATCAGTTCATGTTCTACGACATCATTCGCCTGCTTGACGAAGAACATGCCAACGTTGTGCATGCTAACTTTTCAACTTTGGGAGACTCAACATTTCACATAGTACGTGCAGAAGTACGTAAAACTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03320 map03320
Pfam Domains
Protein Families
HLH ubiquitin

Protein Analysis

166

Amino Acids

19.23

Weight (kDa)

9.52

Isoelectric Point (pI)

53.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 15 - 62 7.8e-09 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 236
AccB1I GGYRCC 1 cut(s) 298
AclI AACGTT 1 cut(s) 420
AclWI GGATC 1 cut(s) 12
AfaI GTAC 3 cut(s) 345, 471, 483
AgsI TTSAA 3 cut(s) 33, 44, 441
AluBI AGCT 4 cut(s) 199, 208, 228, 495
AluI AGCT 4 cut(s) 199, 208, 228, 495
Alw21I GWGCWC 1 cut(s) 284
Alw26I GTCTC 1 cut(s) 444
AlwI GGATC 1 cut(s) 12
ApeKI GCWGC 1 cut(s) 199
AspLEI GCGC 2 cut(s) 237, 293
BanI GGYRCC 1 cut(s) 298
Bbv12I GWGCWC 1 cut(s) 284
BbvI GCAGC 1 cut(s) 186
BcgI CGANNNNNNTGC 2 cut(s) 395, 429
BcoDI GTCTC 1 cut(s) 444
BfaI CTAG 3 cut(s) 240, 335, 492
BfmI CTRYAG 2 cut(s) 200, 258
BisI GCNGC 1 cut(s) 200
BlsI GCNGC 1 cut(s) 201
BmcAI AGTACT 1 cut(s) 345
BmiI GGNNCC 2 cut(s) 135, 300
BplI GAGNNNNNCTC 2 cut(s) 311, 343
BsaAI YACGTR 2 cut(s) 473, 485
BsaBI GATNNNNATC 1 cut(s) 9
BsaXI ACNNNNNCTCC 4 cut(s) 61, 89, 91, 119
Bse8I GATNNNNATC 1 cut(s) 9
BseJI GATNNNNATC 1 cut(s) 9
BseRI GAGGAG 1 cut(s) 101
BseXI GCAGC 1 cut(s) 186
BsgI GTGCAG 1 cut(s) 495
BshNI GGYRCC 1 cut(s) 298
BsiHKAI GWGCWC 1 cut(s) 284
BsmAI GTCTC 1 cut(s) 444
Bsp1286I GDGCHC 1 cut(s) 284
Bsp143I GATC 3 cut(s) 4, 151, 248
BspLI GGNNCC 2 cut(s) 135, 300
BspMAI CTGCAG 1 cut(s) 204
BspPI GGATC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 298
BssMI GATC 3 cut(s) 4, 151, 248
BstBAI YACGTR 2 cut(s) 473, 485
BstC8I GCNNGC 3 cut(s) 265, 398, 429
BstDEI CTNAG 2 cut(s) 140, 358
BstHHI GCGC 2 cut(s) 237, 293
BstKTI GATC 3 cut(s) 7, 154, 251
BstMAI GTCTC 1 cut(s) 444
BstMBI GATC 3 cut(s) 4, 151, 248
BstMWI GCNNNNNNNGC 3 cut(s) 205, 234, 288
BstNSI RCATGY 3 cut(s) 267, 416, 431
BstSFI CTRYAG 2 cut(s) 200, 258
BstSNI TACGTA 1 cut(s) 485
BstV1I GCAGC 1 cut(s) 186
Cac8I GCNNGC 3 cut(s) 265, 398, 429
CfoI GCGC 2 cut(s) 237, 293
Csp6I GTAC 3 cut(s) 344, 470, 482
CviAII CATG 6 cut(s) 14, 264, 317, 376, 413, 428
CviJI RGCY 8 cut(s) 136, 167, 186, 199, 208, 228, 357, 495
CviKI_1 RGCY 8 cut(s) 136, 167, 186, 199, 208, 228, 357, 495
CviQI GTAC 3 cut(s) 344, 470, 482
DdeI CTNAG 2 cut(s) 140, 358
DpnI GATC 3 cut(s) 6, 153, 250
DpnII GATC 3 cut(s) 4, 151, 248
Eco105I TACGTA 1 cut(s) 485
FaeI CATG 6 cut(s) 17, 267, 320, 379, 416, 431
FalI AAGNNNNNCTT 2 cut(s) 212, 244
FatI CATG 6 cut(s) 13, 263, 316, 375, 412, 427
FauNDI CATATG 1 cut(s) 253
Fnu4HI GCNGC 1 cut(s) 200
Fsp4HI GCNGC 1 cut(s) 200
FspAI RTGCGCAY 1 cut(s) 236
FspBI CTAG 3 cut(s) 240, 335, 492
FspI TGCGCA 1 cut(s) 236
GlaI GCGC 2 cut(s) 236, 292
GluI GCNGC 1 cut(s) 200
HhaI GCGC 2 cut(s) 237, 293
Hin1II CATG 6 cut(s) 17, 267, 320, 379, 416, 431
Hin6I GCGC 2 cut(s) 235, 291
HinP1I GCGC 2 cut(s) 235, 291
HinfI GANTC 4 cut(s) 23, 191, 326, 452
Hpy188I TCNGA 1 cut(s) 147
HpyAV CCTTC 1 cut(s) 214
HpyCH4IV ACGT 3 cut(s) 420, 472, 484
HpyCH4V TGCA 4 cut(s) 17, 202, 427, 476
HpyF10VI GCNNNNNNNGC 3 cut(s) 205, 234, 288
HpyF3I CTNAG 2 cut(s) 140, 358
HpySE526I ACGT 3 cut(s) 420, 472, 484
Hsp92II CATG 6 cut(s) 17, 267, 320, 379, 416, 431
HspAI GCGC 2 cut(s) 235, 291
Kzo9I GATC 3 cut(s) 4, 151, 248
LmnI GCTCC 2 cut(s) 133, 288
LpnPI CCDG 2 cut(s) 52, 410
Lsp1109I GCAGC 1 cut(s) 186
MaeI CTAG 3 cut(s) 240, 335, 492
MaeII ACGT 3 cut(s) 420, 472, 484
MalI GATC 3 cut(s) 6, 153, 250
MboI GATC 3 cut(s) 4, 151, 248
MboII GAAGA 5 cut(s) 18, 21, 73, 229, 419
MhlI GDGCHC 1 cut(s) 284
MlyI GAGTC 3 cut(s) 200, 320, 446
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 5 cut(s) 42, 110, 119, 122, 331
MseI TTAA 2 cut(s) 129, 230
MslI CAYNNNNRTG 1 cut(s) 18
MwoI GCNNNNNNNGC 3 cut(s) 205, 234, 288
NdeI CATATG 1 cut(s) 253
NdeII GATC 3 cut(s) 4, 151, 248
NlaIII CATG 6 cut(s) 17, 267, 320, 379, 416, 431
NlaIV GGNNCC 2 cut(s) 135, 300
NsbI TGCGCA 1 cut(s) 236
NspI RCATGY 3 cut(s) 267, 416, 431
PaeI GCATGC 2 cut(s) 267, 431
PfeI GAWTC 1 cut(s) 23
PkrI GCNGC 1 cut(s) 201
PleI GAGTC 3 cut(s) 199, 320, 446
PpsI GAGTC 3 cut(s) 199, 320, 446
Ppu21I YACGTR 2 cut(s) 473, 485
Psp1406I AACGTT 1 cut(s) 420
PspN4I GGNNCC 2 cut(s) 135, 300
PstI CTGCAG 1 cut(s) 204
RsaI GTAC 3 cut(s) 345, 471, 483
RsaNI GTAC 3 cut(s) 344, 470, 482
RseI CAYNNNNRTG 1 cut(s) 18
SaqAI TTAA 2 cut(s) 129, 230
SatI GCNGC 1 cut(s) 200
Sau3AI GATC 3 cut(s) 4, 151, 248
ScaI AGTACT 1 cut(s) 345
SchI GAGTC 3 cut(s) 200, 320, 446
SduI GDGCHC 1 cut(s) 284
SetI ASST 9 cut(s) 102, 201, 210, 230, 342, 423, 475, 487, 497
SfcI CTRYAG 2 cut(s) 200, 258
SmiMI CAYNNNNRTG 1 cut(s) 18
SnaBI TACGTA 1 cut(s) 485
SphI GCATGC 2 cut(s) 267, 431
SspMI CTAG 3 cut(s) 240, 335, 492
TaiI ACGT 3 cut(s) 423, 475, 487
TaqI TCGA 1 cut(s) 194
TatI WGTACW 1 cut(s) 343
TfiI GAWTC 1 cut(s) 23
Tru1I TTAA 2 cut(s) 129, 230
Tru9I TTAA 2 cut(s) 129, 230
TseI GCWGC 1 cut(s) 199
TspDTI ATGAA 4 cut(s) 36, 95, 177, 364
XceI RCATGY 3 cut(s) 267, 416, 431
XspI CTAG 3 cut(s) 240, 335, 492
ZrmI AGTACT 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.