MD17G1237900.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0010583
Physical Location & Seq
Reverse (-)
28725596 .. 28726429
834 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1237900.v1.1.491

Sequence Viewer

Length: 600 bp
ATGGATCGGAATCATGCAAATGAATCTTCTTCAACCAAAGTTGAAAGGAGGGTTGTTGAGAAGAACAGGAGAAATCGTATGAAACTTCTTTACTCCAACCTCTACTCCCTCCTCCCTAAACAAACTTTTAAGGAGCCACTAAGTCAGACAGATCAAATAGATGAAGCCATAAACTACATAAAAAGCCAAGAGTCGAAGCTGCAGAAGCTAAAGGAGAAGAAGGAGAGCTTAATGCGCACTAGAAAAAGATCATATGCTACAGCATGCGTAAATGTTGAGAGCGCAAAGGCACCCCACATCAAAATCCATGAGATAGACTCCACACTAGAGGTAGTACTGATAAGTGGCTTAGAAAATCATCAGTTCATTTTCTACGACATCATTCGCCTGCTTGACGAAGAACATGCCAACGTTGTGCATGCTAACTTTTCAACTTTGGGAGACTCAACATTTCACATAGTACGTGCAGAAATAGGCAAATCTATGTCTGAGTTTGGGGCTGCAAGAATAACTGAAAAACTTACAGATTTGTCAATGGATCCACCAGCGACCAAGAATCACAGCAAGAATACATTTAAGACTTCGAATTCCAGTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03320 map03320
Pfam Domains
Protein Families
HLH ubiquitin

Protein Analysis

200

Amino Acids

22.81

Weight (kDa)

9.21

Isoelectric Point (pI)

51.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 15 - 62 1.1e-08 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 236
AccB1I GGYRCC 1 cut(s) 289
AclI AACGTT 1 cut(s) 411
AclWI GGATC 3 cut(s) 12, 533, 546
AcsI RAATTY 1 cut(s) 586
AfaI GTAC 2 cut(s) 336, 462
AgsI TTSAA 3 cut(s) 33, 44, 432
AluBI AGCT 3 cut(s) 199, 208, 228
AluI AGCT 3 cut(s) 199, 208, 228
Alw26I GTCTC 1 cut(s) 435
AlwI GGATC 3 cut(s) 12, 533, 546
AlwNI CAGNNNCTG 1 cut(s) 597
ApeKI GCWGC 2 cut(s) 199, 500
ApoI RAATTY 1 cut(s) 586
AspLEI GCGC 2 cut(s) 237, 284
AsuII TTCGAA 1 cut(s) 584
BamHI GGATCC 1 cut(s) 538
BanI GGYRCC 1 cut(s) 289
BbvI GCAGC 2 cut(s) 186, 487
BcgI CGANNNNNNTGC 2 cut(s) 386, 420
BcoDI GTCTC 1 cut(s) 435
BfaI CTAG 2 cut(s) 240, 326
BfmI CTRYAG 2 cut(s) 200, 258
BisI GCNGC 2 cut(s) 200, 501
BlsI GCNGC 2 cut(s) 201, 502
BmcAI AGTACT 1 cut(s) 336
BmiI GGNNCC 3 cut(s) 135, 291, 540
BplI GAGNNNNNCTC 2 cut(s) 302, 334
Bpu14I TTCGAA 1 cut(s) 584
BsaAI YACGTR 1 cut(s) 464
BsaBI GATNNNNATC 1 cut(s) 9
BsaXI ACNNNNNCTCC 4 cut(s) 61, 89, 91, 119
Bse1I ACTGG 1 cut(s) 591
Bse8I GATNNNNATC 1 cut(s) 9
BseJI GATNNNNATC 1 cut(s) 9
BseMII CTCAG 1 cut(s) 480
BseNI ACTGG 1 cut(s) 591
BseRI GAGGAG 1 cut(s) 101
BseXI GCAGC 2 cut(s) 186, 487
BsgI GTGCAG 1 cut(s) 486
BshNI GGYRCC 1 cut(s) 289
BsmAI GTCTC 1 cut(s) 435
Bsp119I TTCGAA 1 cut(s) 584
Bsp143I GATC 4 cut(s) 4, 151, 248, 538
BspCNI CTCAG 1 cut(s) 481
BspLI GGNNCC 3 cut(s) 135, 291, 540
BspMAI CTGCAG 1 cut(s) 204
BspPI GGATC 3 cut(s) 12, 533, 546
BspT104I TTCGAA 1 cut(s) 584
BspT107I GGYRCC 1 cut(s) 289
BsrI ACTGG 1 cut(s) 591
BssMI GATC 4 cut(s) 4, 151, 248, 538
BstBAI YACGTR 1 cut(s) 464
BstBI TTCGAA 1 cut(s) 584
BstC8I GCNNGC 3 cut(s) 265, 389, 420
BstDEI CTNAG 3 cut(s) 140, 349, 489
BstHHI GCGC 2 cut(s) 237, 284
BstKTI GATC 4 cut(s) 7, 154, 251, 541
BstMAI GTCTC 1 cut(s) 435
BstMBI GATC 4 cut(s) 4, 151, 248, 538
BstMWI GCNNNNNNNGC 2 cut(s) 205, 234
BstNSI RCATGY 3 cut(s) 267, 407, 422
BstSFI CTRYAG 2 cut(s) 200, 258
BstV1I GCAGC 2 cut(s) 186, 487
BstX2I RGATCY 1 cut(s) 538
BstYI RGATCY 1 cut(s) 538
Cac8I GCNNGC 3 cut(s) 265, 389, 420
CaiI CAGNNNCTG 1 cut(s) 597
CfoI GCGC 2 cut(s) 237, 284
Csp6I GTAC 2 cut(s) 335, 461
CviAII CATG 5 cut(s) 14, 264, 308, 404, 419
CviJI RGCY 8 cut(s) 136, 167, 186, 199, 208, 228, 348, 500
CviKI_1 RGCY 8 cut(s) 136, 167, 186, 199, 208, 228, 348, 500
CviQI GTAC 2 cut(s) 335, 461
DdeI CTNAG 3 cut(s) 140, 349, 489
DpnI GATC 4 cut(s) 6, 153, 250, 540
DpnII GATC 4 cut(s) 4, 151, 248, 538
EcoRI GAATTC 1 cut(s) 586
FaeI CATG 5 cut(s) 17, 267, 311, 407, 422
FalI AAGNNNNNCTT 2 cut(s) 212, 244
FatI CATG 5 cut(s) 13, 263, 307, 403, 418
FauNDI CATATG 1 cut(s) 253
Fnu4HI GCNGC 2 cut(s) 200, 501
Fsp4HI GCNGC 2 cut(s) 200, 501
FspAI RTGCGCAY 1 cut(s) 236
FspBI CTAG 2 cut(s) 240, 326
FspI TGCGCA 1 cut(s) 236
GlaI GCGC 2 cut(s) 236, 283
GluI GCNGC 2 cut(s) 200, 501
HhaI GCGC 2 cut(s) 237, 284
Hin1II CATG 5 cut(s) 17, 267, 311, 407, 422
Hin6I GCGC 2 cut(s) 235, 282
HinP1I GCGC 2 cut(s) 235, 282
HinfI GANTC 6 cut(s) 10, 23, 191, 317, 443, 556
Hpy188I TCNGA 4 cut(s) 9, 147, 490, 599
HpyAV CCTTC 1 cut(s) 214
HpyCH4IV ACGT 2 cut(s) 411, 463
HpyCH4V TGCA 5 cut(s) 17, 202, 418, 467, 503
HpyF10VI GCNNNNNNNGC 2 cut(s) 205, 234
HpyF3I CTNAG 3 cut(s) 140, 349, 489
HpySE526I ACGT 2 cut(s) 411, 463
Hsp92II CATG 5 cut(s) 17, 267, 311, 407, 422
HspAI GCGC 2 cut(s) 235, 282
Kzo9I GATC 4 cut(s) 4, 151, 248, 538
LmnI GCTCC 1 cut(s) 133
LpnPI CCDG 3 cut(s) 52, 401, 558
Lsp1109I GCAGC 2 cut(s) 186, 487
MaeI CTAG 2 cut(s) 240, 326
MaeII ACGT 2 cut(s) 411, 463
MalI GATC 4 cut(s) 6, 153, 250, 540
MboI GATC 4 cut(s) 4, 151, 248, 538
MboII GAAGA 5 cut(s) 18, 21, 73, 229, 410
MflI RGATCY 1 cut(s) 538
MluCI AATT 1 cut(s) 586
MlyI GAGTC 3 cut(s) 200, 311, 437
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 5 cut(s) 42, 110, 119, 122, 322
MseI TTAA 3 cut(s) 129, 230, 576
MslI CAYNNNNRTG 1 cut(s) 18
MwoI GCNNNNNNNGC 2 cut(s) 205, 234
NdeI CATATG 1 cut(s) 253
NdeII GATC 4 cut(s) 4, 151, 248, 538
NlaIII CATG 5 cut(s) 17, 267, 311, 407, 422
NlaIV GGNNCC 3 cut(s) 135, 291, 540
NsbI TGCGCA 1 cut(s) 236
NspI RCATGY 3 cut(s) 267, 407, 422
NspV TTCGAA 1 cut(s) 584
PaeI GCATGC 2 cut(s) 267, 422
PfeI GAWTC 3 cut(s) 10, 23, 556
PkrI GCNGC 2 cut(s) 201, 502
PleI GAGTC 3 cut(s) 199, 311, 437
PpsI GAGTC 3 cut(s) 199, 311, 437
Ppu21I YACGTR 1 cut(s) 464
Psp1406I AACGTT 1 cut(s) 411
PspN4I GGNNCC 3 cut(s) 135, 291, 540
PstI CTGCAG 1 cut(s) 204
PstNI CAGNNNCTG 1 cut(s) 597
PsuI RGATCY 1 cut(s) 538
RsaI GTAC 2 cut(s) 336, 462
RsaNI GTAC 2 cut(s) 335, 461
RseI CAYNNNNRTG 1 cut(s) 18
SaqAI TTAA 3 cut(s) 129, 230, 576
SatI GCNGC 2 cut(s) 200, 501
Sau3AI GATC 4 cut(s) 4, 151, 248, 538
ScaI AGTACT 1 cut(s) 336
SchI GAGTC 3 cut(s) 200, 311, 437
SetI ASST 7 cut(s) 102, 201, 210, 230, 333, 414, 466
SfcI CTRYAG 2 cut(s) 200, 258
SfuI TTCGAA 1 cut(s) 584
SmiMI CAYNNNNRTG 1 cut(s) 18
SphI GCATGC 2 cut(s) 267, 422
Sse9I AATT 1 cut(s) 586
SspMI CTAG 2 cut(s) 240, 326
TaiI ACGT 2 cut(s) 414, 466
TaqI TCGA 2 cut(s) 194, 584
TasI AATT 1 cut(s) 586
TatI WGTACW 1 cut(s) 334
TfiI GAWTC 3 cut(s) 10, 23, 556
Tru1I TTAA 3 cut(s) 129, 230, 576
Tru9I TTAA 3 cut(s) 129, 230, 576
TseI GCWGC 2 cut(s) 199, 500
TspDTI ATGAA 4 cut(s) 36, 95, 177, 355
XapI RAATTY 1 cut(s) 586
XceI RCATGY 3 cut(s) 267, 407, 422
XspI CTAG 2 cut(s) 240, 326
ZrmI AGTACT 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.