Rorug02G0264800

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0010583
Physical Location & Seq
Forward (+)
27953294 .. 27959308
6015 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0264800.1

Sequence Viewer

Length: 1605 bp
ATGGACTCACCAAACCTTGCGGTTGCTTCAGCACAAGTGGGAGGCGGAGGAATGAGGAAGAGTTCAACCCTTATTTGTGCTCCGATCGTGGCCGAATCGGTGGCCGAGATGGTGGTTGAGATTGGCAGGGCAAAGGCCGCTGGTGCTGACCTTGTGGAGATTCGATTGGACCATTTGAAGGTCTTCAATTGCAATCAAGATGTCAAGACCCTAATTGATCAATCCCCTTTGCCCACTCTTTTCACTTATAGACCAAAGTGGGAAGGTGGTCAGTATGATGGCGATGAAAAGAGCAGATTAGATGCGCTTCGACTAGCTATGGAGTTGGGGGCAGATTACATTGATGTTGAGCTTCAGGTTGCTCAAGAATTTATTGACTCCATACAAGACAAGAAGCCTGAAAAGCTAAAAGTTATTGTTTCTTCTCACAACTATCAAGAAACTCCATCTGTTGAGGCTCTTGGCAATCTTGTTGCAAAAATACAAGCCACTGGAGCTGATATAGTGAAGATTGCGACCTTTGCCTTGGATATCACTGATGTCGCTCGCATTTTTCAAATAACCGTGCATTCTCAAGTAAGCAATGTCCCAATAATTGGACTTGTAATGGGTGAAAGGGGTTTGATTTCACGGATACTTTGTGCCAAATTTGGTGGTTATCTTACATTTGGCACAATTGATTCAGGAGCAGTTTCAGCTCCTGGTCAACCTACTATCAAGGATATCTTGCATCTATACAATTTCAGACAGATAGGGCCTGACACAAAAGTGTTCGGTATTATTGGAAAGCCCGTCAGCCACAGCAAATCGCCTATTTTGCATAATGAAGGATTCAAGTCAGTGGGTTTCAATGGAGTTTATGTACATTTGTTGGTGGATGATATTGCCAAATTTCTCAAGACATACTCATCTGCGGATTTTGCTGGTTTCAGTGTTACAATTCCTCACAAGGAAGCTGCACTTAAGTGCTGTGACGAGGTTGATCCAGTTGCAAAGTCAATAGGAGCTATTAATTGCATTATAAGGAGACCAACTGATGGAAAGTTATATGGATTAAATACGGACTATGTCGGGGCTATCTCTGCCATAGAGGATGGACTGCGAGGTTCACTCAACAGTTCCCTTGTAACTGGTTCCCCTTTAGCTGGTAGGCTATTTGTTGTCATAGGTGCCGGAGGTGCTGGCAAGGCCCTTGCTTATGGCGCAAAACAGAAGGGAGCAAGGATTGTGATTGCCAACCGCACCTATGATCGAGCCAGAGAAATTGCAGACACAATTGGAGGACAGGCTTTGTCTATTTCTGATCTAGATAAATTCCACCCAGAGGATGGCATGATTCTTGCAAACACAACATCTATTGGAATGCAGCCCAAAGTTAATGAGACACCCATTTCTAAGCATGCTCTGAGATCTTACGCCTTGGTGTTCGATGCCGTTTACACCCCCAAAATAACCAGACTCTTGAAGGAAGCAGAAGAGTCTGGTGCCATAGTTGTTAGTGGGTCGGAGATGTTCATTGGACAGGCATATGAACAGTTCGAGAGGTTCACTGGATTGCCTGCGCCAAAGGAACTCTTCAGGAAAGTTGTGGAGAGCACCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

534

Amino Acids

57.3

Weight (kDa)

6.24

Isoelectric Point (pI)

26.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHquinase_I PF01487 26 - 246 4.8e-70 Type I 3-dehydroquinase
Shikimate_dh_N PF08501 260 - 340 6.4e-27 Shikimate dehydrogenase substrate binding domain
Shikimate_DH PF01488 382 - 452 6.3e-08 Shikimate / quinate 5-dehydrogenase
Sacchrp_dh_NADP PF03435 387 - 499 3e-07 Saccharopine dehydrogenase NADP binding domain
SDH_C PF18317 501 - 531 1.5e-09 Shikimate 5'-dehydrogenase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1022
AccB1I GGYRCC 2 cut(s) 1169, 1484
AccB7I CCANNNNNTGG 3 cut(s) 596, 1037, 1328
AciI CCGC 5 cut(s) 20, 45, 138, 914, 1240
AclWI GGATC 1 cut(s) 977
AcoI YGGCCR 2 cut(s) 90, 102
AcsI RAATTY 4 cut(s) 368, 647, 890, 1313
AcuI CTGAAG 3 cut(s) 12, 338, 1561
AfaI GTAC 1 cut(s) 864
AfiI CCNNNNNNNGG 6 cut(s) 178, 596, 1037, 1145, 1324, 1328
AflII CTTAAG 1 cut(s) 962
AgsI TTSAA 7 cut(s) 66, 178, 187, 557, 835, 850, 1465
AjnI CCWGG 1 cut(s) 700
AleI CACNNNNGTG 2 cut(s) 767, 964
AluBI AGCT 8 cut(s) 317, 352, 406, 497, 698, 956, 1007, 1145
AluI AGCT 8 cut(s) 317, 352, 406, 497, 698, 956, 1007, 1145
Alw21I GWGCWC 2 cut(s) 82, 1598
Alw26I GTCTC 2 cut(s) 1021, 1376
AlwI GGATC 1 cut(s) 977
AlwNI CAGNNNCTG 1 cut(s) 701
AoxI GGCC 5 cut(s) 90, 102, 135, 755, 1188
ApeKI GCWGC 2 cut(s) 956, 1366
ApoI RAATTY 4 cut(s) 368, 647, 890, 1313
AseI ATTAAT 1 cut(s) 1011
Asp700I GAANNNNTTC 1 cut(s) 182
AspLEI GCGC 3 cut(s) 307, 1205, 1564
AspS9I GGNCC 3 cut(s) 169, 755, 1189
AsuHPI GGTGA 1 cut(s) 623
AvaII GGWCC 1 cut(s) 169
BanI GGYRCC 2 cut(s) 1169, 1484
BbsI GAAGAC 1 cut(s) 175
Bbv12I GWGCWC 2 cut(s) 82, 1598
BbvI GCAGC 2 cut(s) 943, 1378
BccI CCATC 6 cut(s) 103, 272, 454, 1031, 1088, 1322
BceAI ACGGC 1 cut(s) 1418
BciT130I CCWGG 1 cut(s) 702
BciVI GTATCC 1 cut(s) 627
BclI TGATCA 1 cut(s) 217
BcoDI GTCTC 2 cut(s) 1021, 1376
BfaI CTAG 2 cut(s) 314, 1307
BfrI CTTAAG 1 cut(s) 962
BfuI GTATCC 1 cut(s) 627
BglII AGATCT 1 cut(s) 1409
BisI GCNGC 3 cut(s) 138, 957, 1367
BlsI GCNGC 3 cut(s) 139, 958, 1368
Bme1390I CCNGG 1 cut(s) 702
Bme18I GGWCC 1 cut(s) 169
BmgT120I GGNCC 3 cut(s) 169, 755, 1189
BmiI GGNNCC 3 cut(s) 1135, 1171, 1486
BmrFI CCNGG 1 cut(s) 702
BmsI GCATC 3 cut(s) 292, 739, 1420
BpiI GAAGAC 1 cut(s) 175
BplI GAGNNNNNCTC 3 cut(s) 1095, 1127, 1583
BpmI CTGGAG 1 cut(s) 513
BpuEI CTTGAG 3 cut(s) 348, 558, 881
BsaI GGTCTC 1 cut(s) 1021
BsaJI CCNNGG 2 cut(s) 525, 1419
BsaXI ACNNNNNCTCC 2 cut(s) 846, 876
Bsc4I CCNNNNNNNGG 6 cut(s) 178, 596, 1037, 1145, 1324, 1328
Bse1I ACTGG 4 cut(s) 496, 986, 1135, 1555
Bse3DI GCAATG 1 cut(s) 589
BseBI CCWGG 1 cut(s) 702
BseDI CCNNGG 2 cut(s) 525, 1419
BseGI GGATG 3 cut(s) 883, 1099, 1333
BseLI CCNNNNNNNGG 6 cut(s) 178, 596, 1037, 1145, 1324, 1328
BseMI GCAATG 1 cut(s) 589
BseMII CTCAG 1 cut(s) 1397
BseNI ACTGG 4 cut(s) 496, 986, 1135, 1555
BseXI GCAGC 2 cut(s) 943, 1378
BsgI GTGCAG 1 cut(s) 942
Bsh1285I CGRYCG 1 cut(s) 87
BshFI GGCC 5 cut(s) 92, 104, 137, 757, 1190
BshNI GGYRCC 2 cut(s) 1169, 1484
BsiEI CGRYCG 1 cut(s) 87
BsiHKAI GWGCWC 2 cut(s) 82, 1598
BsiSI CCGG 1 cut(s) 1173
BslFI GGGAC 1 cut(s) 572
BslI CCNNNNNNNGG 6 cut(s) 178, 596, 1037, 1145, 1324, 1328
BsmAI GTCTC 2 cut(s) 1021, 1376
BsmFI GGGAC 1 cut(s) 572
BsmI GAATGC 2 cut(s) 568, 1368
BsnI GGCC 5 cut(s) 92, 104, 137, 757, 1190
Bso31I GGTCTC 1 cut(s) 1021
Bsp1286I GDGCHC 2 cut(s) 82, 1598
Bsp1407I TGTACA 1 cut(s) 862
Bsp143I GATC 6 cut(s) 84, 217, 982, 1249, 1303, 1409
BspACI CCGC 5 cut(s) 20, 45, 138, 914, 1240
BspANI GGCC 5 cut(s) 92, 104, 137, 757, 1190
BspCNI CTCAG 1 cut(s) 1398
BspLI GGNNCC 3 cut(s) 1135, 1171, 1486
BspPI GGATC 1 cut(s) 977
BspT107I GGYRCC 2 cut(s) 1169, 1484
BspTI CTTAAG 1 cut(s) 962
BspTNI GGTCTC 1 cut(s) 1021
BsrDI GCAATG 1 cut(s) 589
BsrGI TGTACA 1 cut(s) 862
BsrI ACTGG 4 cut(s) 496, 986, 1135, 1555
BssECI CCNNGG 2 cut(s) 525, 1419
BssMI GATC 6 cut(s) 84, 217, 982, 1249, 1303, 1409
BssT1I CCWWGG 2 cut(s) 525, 1419
Bst2UI CCWGG 1 cut(s) 702
Bst4CI ACNGT 3 cut(s) 565, 1118, 1536
Bst6I CTCTTC 3 cut(s) 53, 1470, 1580
BstAFI CTTAAG 1 cut(s) 962
BstAUI TGTACA 1 cut(s) 862
BstC8I GCNNGC 4 cut(s) 547, 1183, 1401, 1560
BstDEI CTNAG 2 cut(s) 1395, 1406
BstF5I GGATG 3 cut(s) 883, 1099, 1333
BstHHI GCGC 3 cut(s) 307, 1205, 1564
BstKTI GATC 6 cut(s) 87, 220, 985, 1252, 1306, 1412
BstMAI GTCTC 2 cut(s) 1021, 1376
BstMBI GATC 6 cut(s) 84, 217, 982, 1249, 1303, 1409
BstMCI CGRYCG 1 cut(s) 87
BstNI CCWGG 1 cut(s) 702
BstNSI RCATGY 1 cut(s) 1403
BstSCI CCNGG 1 cut(s) 700
BstV1I GCAGC 2 cut(s) 943, 1378
BstV2I GAAGAC 1 cut(s) 175
BstX2I RGATCY 1 cut(s) 1409
BstYI RGATCY 1 cut(s) 1409
BsuI GTATCC 1 cut(s) 627
BsuRI GGCC 5 cut(s) 92, 104, 137, 757, 1190
BtgZI GCGATG 1 cut(s) 297
BtsCI GGATG 3 cut(s) 883, 1099, 1333
BtsIMutI CAGTG 5 cut(s) 489, 534, 846, 937, 1548
Cac8I GCNNGC 4 cut(s) 547, 1183, 1401, 1560
CaiI CAGNNNCTG 1 cut(s) 701
CfoI GCGC 3 cut(s) 307, 1205, 1564
Cfr13I GGNCC 3 cut(s) 169, 755, 1189
Csp6I GTAC 1 cut(s) 863
CspCI CAANNNNNGTGG 2 cut(s) 634, 669
CviAII CATG 2 cut(s) 1333, 1400
CviQI GTAC 1 cut(s) 863
DdeI CTNAG 2 cut(s) 1395, 1406
DpnI GATC 6 cut(s) 86, 219, 984, 1251, 1305, 1411
DpnII GATC 6 cut(s) 84, 217, 982, 1249, 1303, 1409
EaeI YGGCCR 2 cut(s) 90, 102
Eam1104I CTCTTC 3 cut(s) 53, 1470, 1580
EarI CTCTTC 3 cut(s) 53, 1470, 1580
EciI GGCGGA 1 cut(s) 60
Eco130I CCWWGG 2 cut(s) 525, 1419
Eco31I GGTCTC 1 cut(s) 1021
Eco32I GATATC 2 cut(s) 532, 724
Eco47I GGWCC 1 cut(s) 169
Eco57I CTGAAG 3 cut(s) 12, 338, 1561
EcoO109I RGGNCCY 2 cut(s) 755, 1189
EcoRII CCWGG 1 cut(s) 700
EcoRV GATATC 2 cut(s) 532, 724
EcoT14I CCWWGG 2 cut(s) 525, 1419
ErhI CCWWGG 2 cut(s) 525, 1419
FaeI CATG 2 cut(s) 1336, 1403
FalI AAGNNNNNCTT 6 cut(s) 710, 742, 945, 977, 1559, 1591
FaqI GGGAC 1 cut(s) 572
FatI CATG 2 cut(s) 1332, 1399
FauNDI CATATG 1 cut(s) 1528
FbaI TGATCA 1 cut(s) 217
Fnu4HI GCNGC 3 cut(s) 138, 957, 1367
FokI GGATG 3 cut(s) 890, 1106, 1340
Fsp4HI GCNGC 3 cut(s) 138, 957, 1367
FspBI CTAG 2 cut(s) 314, 1307
GlaI GCGC 3 cut(s) 306, 1204, 1563
GluI GCNGC 3 cut(s) 138, 957, 1367
GsuI CTGGAG 1 cut(s) 513
HaeIII GGCC 5 cut(s) 92, 104, 137, 757, 1190
HapII CCGG 1 cut(s) 1173
HhaI GCGC 3 cut(s) 307, 1205, 1564
Hin1II CATG 2 cut(s) 1336, 1403
Hin6I GCGC 3 cut(s) 305, 1203, 1562
HinP1I GCGC 3 cut(s) 305, 1203, 1562
HincII GTYRAC 1 cut(s) 707
HindII GTYRAC 1 cut(s) 707
HinfI GANTC 9 cut(s) 5, 95, 160, 377, 680, 831, 1336, 1458, 1478
HpaII CCGG 1 cut(s) 1173
HphI GGTGA 1 cut(s) 623
Hpy166II GTNNAC 4 cut(s) 707, 1109, 1438, 1548
Hpy188I TCNGA 5 cut(s) 84, 746, 1303, 1407, 1507
Hpy8I GTNNAC 4 cut(s) 707, 1109, 1438, 1548
HpyAV CCTTC 5 cut(s) 172, 257, 821, 1207, 1459
HpyCH4III ACNGT 3 cut(s) 565, 1118, 1536
HpyF3I CTNAG 2 cut(s) 1395, 1406
Hsp92II CATG 2 cut(s) 1336, 1403
HspAI GCGC 3 cut(s) 305, 1203, 1562
Ksp22I TGATCA 1 cut(s) 217
Kzo9I GATC 6 cut(s) 84, 217, 982, 1249, 1303, 1409
LmnI GCTCC 6 cut(s) 85, 494, 686, 703, 1004, 1217
Lsp1109I GCAGC 2 cut(s) 943, 1378
LweI GCATC 3 cut(s) 292, 739, 1420
MaeI CTAG 2 cut(s) 314, 1307
MaeIII GTNAC 3 cut(s) 934, 971, 1126
MalI GATC 6 cut(s) 86, 219, 984, 1251, 1305, 1411
MboI GATC 6 cut(s) 84, 217, 982, 1249, 1303, 1409
MboII GAAGA 6 cut(s) 70, 175, 414, 520, 1487, 1567
MfeI CAATTG 3 cut(s) 187, 675, 1275
MflI RGATCY 1 cut(s) 1409
MhlI GDGCHC 2 cut(s) 82, 1598
MlyI GAGTC 3 cut(s) 371, 1452, 1487
MmeI TCCRAC 1 cut(s) 1485
MroXI GAANNNNTTC 1 cut(s) 182
MseI TTAA 4 cut(s) 963, 1011, 1055, 1377
MslI CAYNNNNRTG 2 cut(s) 767, 964
MspA1I CMGCKG 1 cut(s) 140
MspCI CTTAAG 1 cut(s) 962
MspI CCGG 1 cut(s) 1173
MspR9I CCNGG 1 cut(s) 702
MunI CAATTG 3 cut(s) 187, 675, 1275
Mva1269I GAATGC 2 cut(s) 568, 1368
MvaI CCWGG 1 cut(s) 702
NdeI CATATG 1 cut(s) 1528
NdeII GATC 6 cut(s) 84, 217, 982, 1249, 1303, 1409
NlaIII CATG 2 cut(s) 1336, 1403
NlaIV GGNNCC 3 cut(s) 1135, 1171, 1486
NmeAIII GCCGAG 1 cut(s) 130
NmuCI GTSAC 1 cut(s) 971
NspI RCATGY 1 cut(s) 1403
OliI CACNNNNGTG 2 cut(s) 767, 964
PaeI GCATGC 1 cut(s) 1403
PctI GAATGC 2 cut(s) 568, 1368
PdmI GAANNNNTTC 1 cut(s) 182
PfeI GAWTC 5 cut(s) 95, 160, 680, 831, 1336
PflFI GACNNNGTC 1 cut(s) 1067
PflMI CCANNNNNTGG 3 cut(s) 596, 1037, 1328
PkrI GCNGC 3 cut(s) 139, 958, 1368
Ple19I CGATCG 1 cut(s) 87
PleI GAGTC 3 cut(s) 371, 1452, 1486
PpsI GAGTC 3 cut(s) 371, 1452, 1486
PshBI ATTAAT 1 cut(s) 1011
PsiI TTATAA 1 cut(s) 1022
Psp6I CCWGG 1 cut(s) 700
PspGI CCWGG 1 cut(s) 700
PspN4I GGNNCC 3 cut(s) 1135, 1171, 1486
PspPI GGNCC 3 cut(s) 169, 755, 1189
PstNI CAGNNNCTG 1 cut(s) 701
PsuI RGATCY 1 cut(s) 1409
PsyI GACNNNGTC 1 cut(s) 1067
PvuI CGATCG 1 cut(s) 87
RsaI GTAC 1 cut(s) 864
RsaNI GTAC 1 cut(s) 863
RseI CAYNNNNRTG 2 cut(s) 767, 964
SaqAI TTAA 4 cut(s) 963, 1011, 1055, 1377
SatI GCNGC 3 cut(s) 138, 957, 1367
Sau3AI GATC 6 cut(s) 84, 217, 982, 1249, 1303, 1409
Sau96I GGNCC 3 cut(s) 169, 755, 1189
SchI GAGTC 3 cut(s) 371, 1452, 1487
ScrFI CCNGG 1 cut(s) 702
SduI GDGCHC 2 cut(s) 82, 1598
SfaNI GCATC 3 cut(s) 292, 739, 1420
SinI GGWCC 1 cut(s) 169
SmiMI CAYNNNNRTG 2 cut(s) 767, 964
SmlI CTYRAG 4 cut(s) 363, 573, 896, 962
SmoI CTYRAG 4 cut(s) 363, 573, 896, 962
SphI GCATGC 1 cut(s) 1403
SsiI CCGC 5 cut(s) 20, 45, 138, 914, 1240
SspMI CTAG 2 cut(s) 314, 1307
StyD4I CCNGG 1 cut(s) 700
StyI CCWWGG 2 cut(s) 525, 1419
TaaI ACNGT 3 cut(s) 565, 1118, 1536
TaqI TCGA 5 cut(s) 163, 310, 1252, 1428, 1539
TatI WGTACW 1 cut(s) 862
TauI GCSGC 1 cut(s) 140
TfiI GAWTC 5 cut(s) 95, 160, 680, 831, 1336
Tru1I TTAA 4 cut(s) 963, 1011, 1055, 1377
Tru9I TTAA 4 cut(s) 963, 1011, 1055, 1377
TscAI CASTG 5 cut(s) 496, 541, 846, 937, 1555
TseFI GTSAC 1 cut(s) 971
TseI GCWGC 2 cut(s) 956, 1366
Tsp45I GTSAC 1 cut(s) 971
TspDTI ATGAA 4 cut(s) 300, 840, 1504, 1545
TspGWI ACGGA 2 cut(s) 646, 1076
TspRI CASTG 5 cut(s) 496, 541, 846, 937, 1555
Tth111I GACNNNGTC 1 cut(s) 1067
Van91I CCANNNNNTGG 3 cut(s) 596, 1037, 1328
Vha464I CTTAAG 1 cut(s) 962
VpaK11BI GGWCC 1 cut(s) 169
VspI ATTAAT 1 cut(s) 1011
XapI RAATTY 4 cut(s) 368, 647, 890, 1313
XbaI TCTAGA 1 cut(s) 1306
XceI RCATGY 1 cut(s) 1403
XcmI CCANNNNNNNNNTGG 1 cut(s) 1325
XmnI GAANNNNTTC 1 cut(s) 182
XspI CTAG 2 cut(s) 314, 1307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.