MD10G1014900.v1.1

proline-rich receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0013267
Physical Location & Seq
Reverse (-)
2013344 .. 2013901
558 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1014900.v1.1.491

Sequence Viewer

Length: 558 bp
ATGCCACCGCCCCCGCCTCATGTACGCCCTCCGCCACCGCAGCTGCCACCACCGTCCCCATCACCGGACAATAATCCCACTGTCATAGTCATAGTGTTCATCTCATTCGGTTCTGTTTTCTTCCTTGCATTCCTTGCAGTTTCCCTGCTTTGTTGCATCAAGAGGAGAAAGAAGAAGACAATCCAAGAAACCAACTTCATTCACATTGACAAACACCGGAAGATGAAGGAAGCAATTGTAGAAGGTCCCAACGGTCCGCAAGCCGTGGTGTTATCGGTCGAGGATGATGTCCACGTCGACGAGGAGATTAGGAAGAATGAGACAATTGCTCACAAGGGGTTGCATGGAAAAGTAAAGAGTTCAGATGTCCAGTCGGATACCGAAATCGTTAACGTCGGCGAACATCGGAACGTGAAGGAAGACATAGAAGGTCCGCATGGATCGAAAGCTGTGGTGCTAACAGTCGAAGACGATGTTCATATCGATGAAATATTAAGGAAGAACGAGAAGGTTGGTAAAGGTTTGCATGGCAATGCAGAGGCAGTGAAAATCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

186

Amino Acids

20.4

Weight (kDa)

6.36

Isoelectric Point (pI)

49.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013267)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43790
fragaria_vesca FvH4_2g14670
malus_domestica MD05G1014900.v1.1 MD10G1014900.v1.1
prunus_persica Prupe.8G018800_v2.0.a1
pyrus_communis pycom05g00820 pycom10g00920
rosa_chinensis RchiOBHm_Chr6g0277141
rosa_laevigata RLG00000013328
rosa_multiflora Rmu_sc0003539.1_g000022 Rmu_sc0006244.1_g000041
rosa_roxburghii Rroxscaffold_7G00191570
rosa_rugosa Rorug06G0106500
rosa_samantha Rh6AG217300 Rh6BG221300 Rh6CG224200 Rh6DG213900
rosa_wichuraiana Rw6G018970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 297
AciI CCGC 6 cut(s) 8, 14, 32, 38, 257, 434
AclWI GGATC 1 cut(s) 448
AfaI GTAC 1 cut(s) 24
AfiI CCNNNNNNNGG 1 cut(s) 64
AjiI CACGTC 1 cut(s) 295
AluBI AGCT 2 cut(s) 43, 449
AluI AGCT 2 cut(s) 43, 449
Alw26I GTCTC 1 cut(s) 314
AlwI GGATC 1 cut(s) 448
ApeKI GCWGC 2 cut(s) 40, 43
AspS9I GGNCC 3 cut(s) 245, 254, 431
AsuHPI GGTGA 1 cut(s) 54
AvaII GGWCC 3 cut(s) 245, 254, 431
BbsI GAAGAC 3 cut(s) 182, 426, 474
BbvI GCAGC 2 cut(s) 30, 52
BccI CCATC 1 cut(s) 67
BceAI ACGGC 1 cut(s) 248
BciVI GTATCC 1 cut(s) 370
BcoDI GTCTC 1 cut(s) 314
BfuI GTATCC 1 cut(s) 370
BisI GCNGC 2 cut(s) 41, 44
BlsI GCNGC 2 cut(s) 42, 45
Bme18I GGWCC 3 cut(s) 245, 254, 431
BmgBI CACGTC 1 cut(s) 295
BmgT120I GGNCC 3 cut(s) 245, 254, 431
BmiI GGNNCC 1 cut(s) 247
BmsI GCATC 1 cut(s) 165
BpiI GAAGAC 3 cut(s) 182, 426, 474
Bsa29I ATCGAT 2 cut(s) 483, 552
BsaJI CCNNGG 1 cut(s) 264
BsaWI WCCGGW 2 cut(s) 64, 216
Bsc4I CCNNNNNNNGG 1 cut(s) 64
Bse1I ACTGG 1 cut(s) 370
Bse3DI GCAATG 1 cut(s) 538
BseCI ATCGAT 2 cut(s) 483, 552
BseDI CCNNGG 1 cut(s) 264
BseGI GGATG 1 cut(s) 289
BseLI CCNNNNNNNGG 1 cut(s) 64
BseMI GCAATG 1 cut(s) 538
BseNI ACTGG 1 cut(s) 370
BseRI GAGGAG 2 cut(s) 178, 317
BseXI GCAGC 2 cut(s) 30, 52
Bsh1285I CGRYCG 1 cut(s) 279
BshVI ATCGAT 2 cut(s) 483, 552
BsiEI CGRYCG 1 cut(s) 279
BsiSI CCGG 2 cut(s) 65, 217
BslFI GGGAC 2 cut(s) 40, 231
BslI CCNNNNNNNGG 1 cut(s) 64
BsmAI GTCTC 1 cut(s) 314
BsmFI GGGAC 2 cut(s) 40, 231
BsmI GAATGC 1 cut(s) 128
Bsp143I GATC 1 cut(s) 440
BspACI CCGC 6 cut(s) 8, 14, 32, 38, 257, 434
BspDI ATCGAT 2 cut(s) 483, 552
BspLI GGNNCC 1 cut(s) 247
BspPI GGATC 1 cut(s) 448
BsrDI GCAATG 1 cut(s) 538
BsrI ACTGG 1 cut(s) 370
BssECI CCNNGG 1 cut(s) 264
BssMI GATC 1 cut(s) 440
Bst4CI ACNGT 4 cut(s) 54, 82, 254, 463
BstAPI GCANNNNNTGC 1 cut(s) 134
BstC8I GCNNGC 1 cut(s) 261
BstDSI CCRYGG 1 cut(s) 264
BstF5I GGATG 1 cut(s) 289
BstKTI GATC 1 cut(s) 443
BstMAI GTCTC 1 cut(s) 314
BstMBI GATC 1 cut(s) 440
BstMCI CGRYCG 1 cut(s) 279
BstMWI GCNNNNNNNGC 2 cut(s) 40, 134
BstV1I GCAGC 2 cut(s) 30, 52
BstV2I GAAGAC 3 cut(s) 182, 426, 474
Bsu15I ATCGAT 2 cut(s) 483, 552
BsuI GTATCC 1 cut(s) 370
BsuTUI ATCGAT 2 cut(s) 483, 552
BtgI CCRYGG 1 cut(s) 264
BtrI CACGTC 1 cut(s) 295
BtsCI GGATG 1 cut(s) 289
BtsI GCAGTG 1 cut(s) 549
BtsIMutI CAGTG 2 cut(s) 78, 549
Cac8I GCNNGC 1 cut(s) 261
Cfr13I GGNCC 3 cut(s) 245, 254, 431
ClaI ATCGAT 2 cut(s) 483, 552
CpoI CGGWCCG 1 cut(s) 254
Csp6I GTAC 1 cut(s) 23
CspI CGGWCCG 1 cut(s) 254
CviAII CATG 4 cut(s) 20, 344, 437, 527
CviJI RGCY 3 cut(s) 43, 263, 449
CviKI_1 RGCY 3 cut(s) 43, 263, 449
CviQI GTAC 1 cut(s) 23
DpnI GATC 1 cut(s) 442
DpnII GATC 1 cut(s) 440
EciI GGCGGA 1 cut(s) 21
Eco47I GGWCC 3 cut(s) 245, 254, 431
EcoO109I RGGNCCY 1 cut(s) 245
FaeI CATG 4 cut(s) 23, 347, 440, 530
FaiI YATR 8 cut(s) 21, 86, 92, 345, 425, 438, 480, 528
FaqI GGGAC 2 cut(s) 40, 231
FatI CATG 4 cut(s) 19, 343, 436, 526
FauI CCCGC 1 cut(s) 21
FblI GTMKAC 1 cut(s) 297
Fnu4HI GCNGC 2 cut(s) 41, 44
FokI GGATG 1 cut(s) 296
Fsp4HI GCNGC 2 cut(s) 41, 44
GluI GCNGC 2 cut(s) 41, 44
HapII CCGG 2 cut(s) 65, 217
Hin1II CATG 4 cut(s) 23, 347, 440, 530
HincII GTYRAC 2 cut(s) 298, 391
HindII GTYRAC 2 cut(s) 298, 391
HpaI GTTAAC 1 cut(s) 391
HpaII CCGG 2 cut(s) 65, 217
HphI GGTGA 1 cut(s) 54
Hpy166II GTNNAC 3 cut(s) 292, 298, 391
Hpy188I TCNGA 3 cut(s) 364, 376, 408
Hpy188III TCNNGA 1 cut(s) 160
Hpy8I GTNNAC 3 cut(s) 292, 298, 391
Hpy99I CGWCG 3 cut(s) 299, 302, 398
HpyAV CCTTC 5 cut(s) 220, 236, 409, 422, 502
HpyCH4III ACNGT 4 cut(s) 54, 82, 254, 463
HpyCH4IV ACGT 3 cut(s) 294, 393, 411
HpyCH4V TGCA 6 cut(s) 128, 137, 156, 343, 526, 536
HpyF10VI GCNNNNNNNGC 2 cut(s) 40, 134
HpySE526I ACGT 3 cut(s) 294, 393, 411
Hsp92II CATG 4 cut(s) 23, 347, 440, 530
KspAI GTTAAC 1 cut(s) 391
Kzo9I GATC 1 cut(s) 440
LpnPI CCDG 4 cut(s) 78, 158, 230, 383
Lsp1109I GCAGC 2 cut(s) 30, 52
LweI GCATC 1 cut(s) 165
MaeII ACGT 3 cut(s) 294, 393, 411
MalI GATC 1 cut(s) 442
MboI GATC 1 cut(s) 440
MboII GAAGA 8 cut(s) 112, 184, 187, 232, 325, 431, 479, 511
MfeI CAATTG 2 cut(s) 234, 324
MluCI AATT 2 cut(s) 234, 324
MmeI TCCRAC 1 cut(s) 354
MnlI CCTC 6 cut(s) 27, 39, 156, 274, 295, 532
MseI TTAA 2 cut(s) 390, 494
MslI CAYNNNNRTG 2 cut(s) 483, 531
MspA1I CMGCKG 1 cut(s) 43
MspI CCGG 2 cut(s) 65, 217
MunI CAATTG 2 cut(s) 234, 324
Mva1269I GAATGC 1 cut(s) 128
MwoI GCNNNNNNNGC 2 cut(s) 40, 134
NdeII GATC 1 cut(s) 440
NlaIII CATG 4 cut(s) 23, 347, 440, 530
NlaIV GGNNCC 1 cut(s) 247
PctI GAATGC 1 cut(s) 128
PkrI GCNGC 2 cut(s) 42, 45
PpuMI RGGWCCY 1 cut(s) 245
Psp5II RGGWCCY 1 cut(s) 245
PspN4I GGNNCC 1 cut(s) 247
PspPI GGNCC 3 cut(s) 245, 254, 431
PspPPI RGGWCCY 1 cut(s) 245
PvuII CAGCTG 1 cut(s) 43
RsaI GTAC 1 cut(s) 24
RsaNI GTAC 1 cut(s) 23
RseI CAYNNNNRTG 2 cut(s) 483, 531
Rsr2I CGGWCCG 1 cut(s) 254
RsrII CGGWCCG 1 cut(s) 254
SalI GTCGAC 1 cut(s) 296
SaqAI TTAA 2 cut(s) 390, 494
SatI GCNGC 2 cut(s) 41, 44
Sau3AI GATC 1 cut(s) 440
Sau96I GGNCC 3 cut(s) 245, 254, 431
SetI ASST 9 cut(s) 45, 247, 297, 396, 414, 433, 451, 513, 523
SfaNI GCATC 1 cut(s) 165
SgrDI CGTCGACG 1 cut(s) 296
SinI GGWCC 3 cut(s) 245, 254, 431
SmiMI CAYNNNNRTG 2 cut(s) 483, 531
Sse9I AATT 2 cut(s) 234, 324
SsiI CCGC 6 cut(s) 8, 14, 32, 38, 257, 434
SspI AATATT 1 cut(s) 492
TaaI ACNGT 4 cut(s) 54, 82, 254, 463
TaiI ACGT 3 cut(s) 297, 396, 414
TaqI TCGA 6 cut(s) 279, 297, 443, 465, 483, 552
TaqII GACCGA 1 cut(s) 265
TasI AATT 2 cut(s) 234, 324
Tru1I TTAA 2 cut(s) 390, 494
Tru9I TTAA 2 cut(s) 390, 494
TscAI CASTG 2 cut(s) 85, 549
TseI GCWGC 2 cut(s) 40, 43
TspDTI ATGAA 5 cut(s) 88, 187, 239, 467, 501
TspRI CASTG 2 cut(s) 85, 549
VpaK11BI GGWCC 3 cut(s) 245, 254, 431
XmiI GTMKAC 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.