MD10G1047400.v1.1

Hydroxyproline-rich glycoprotein family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006684
Physical Location & Seq
Forward (+)
6348188 .. 6356700
8513 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1047400.v1.1.491

Sequence Viewer

Length: 1026 bp
ATGTTACGACGACGCCTCACTTTTTCCTCCTTCAACTCCAACTGCCCCCACAAATCTGTATTGAGTGGGTGGCGCCGACGGCCGTACGCTAGTAACTCTAACGCGGCTGCTGCCGAGGCAGAGGCAGAGGGGGAGGCGGAGGCACCGAATGGAAATGTCCCCAAACCCAAAGTTAAGGGGAAAAGGCTTCCCAAAGCGGAGCGCCAAGTTATGCTTGAATCCTTTGTCAACCAGTACAGAGCAAAGAATGCCGGGGCATTCCCTACCGTTTCATATGCTCAGAAAGAATTGGGTGGCTCTTACTATGTCGTGAAGAGCATCCTTCAGGAGCTGCAGTACAGAGCTAAGATGTCTCCCTCGTGTCCTGCCATTGAGGATCTGCCAGTACAACAGAAAGTTAGCGAAACTGAGCCTTTGACTCATGTTGAAGAGAAATCAACCGGTGAGGTGCCAGCTGATTCCAATGCTCAAAATGTTTCTGCGGCGATGTATGATTTGCCAAGGCAAGAAGAAGGTAGGGAAATTAAATCTGTGACTCAAGTTGAAGAGAATTCAACCAGTGAGATGTCAGCTGATGTCAGTGTTCAAATTGATTCTAAGGCGACATATGATACTGATCTTAACGGTGCAAATGGCGGGAATTTAGAACCCAAAGAAGAGGCCCAGACTTCCTCCTCAGTTAACGTTGTGTCTGAAGAGGTTACCACTCCAGGACCGAAGAGTAGTCAAACAGAGGAAGCCCAAGCTGATCAACATGAATTTGTTGCAAAAGAAAAAGATCTGCCAATAGGGGAAAGTAGTAGAGTTGCTCATCCAAGTTTAGGTGATACAAAGGATATGATAAAACAATATGATGACTCAAAGCGTAAAACAGAGCAATATAAAGAGGTCCCTGAGCCAGACAAACTTTCCAAAGAGCGTTCCAGTACGCAGGCTGAAGATGCAGAGCCATCGAAGACATCATCTCTTTGGGGGAATATGAAGTCATTTGCCAATGGCATTATTAGTCTTTTCAGAAAGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

342

Amino Acids

37.51

Weight (kDa)

5.36

Isoelectric Point (pI)

60.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HTH_AT3G52170 PF25896 64 - 113 1.7e-24 Helix-turn-helix domain in AT3G52170-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 72, 142, 448
AccII CGCG 1 cut(s) 104
AciI CCGC 5 cut(s) 104, 137, 197, 482, 636
AclI AACGTT 1 cut(s) 684
AclWI GGATC 1 cut(s) 384
AcoI YGGCCR 1 cut(s) 80
AcsI RAATTY 3 cut(s) 550, 640, 758
AcuI CTGAAG 3 cut(s) 308, 714, 957
AcyI GRCGYC 2 cut(s) 13, 73
AfaI GTAC 5 cut(s) 86, 236, 338, 387, 928
AfiI CCNNNNNNNGG 1 cut(s) 821
AgeI ACCGGT 1 cut(s) 440
AgsI TTSAA 6 cut(s) 34, 218, 428, 545, 555, 587
AjnI CCWGG 1 cut(s) 709
AluBI AGCT 5 cut(s) 331, 344, 455, 572, 746
AluI AGCT 5 cut(s) 331, 344, 455, 572, 746
Alw26I GTCTC 1 cut(s) 357
AlwI GGATC 1 cut(s) 384
AlwNI CAGNNNCTG 1 cut(s) 331
AoxI GGCC 2 cut(s) 80, 660
ApeKI GCWGC 3 cut(s) 107, 110, 331
ApoI RAATTY 3 cut(s) 550, 640, 758
AsiGI ACCGGT 1 cut(s) 440
AspLEI GCGC 2 cut(s) 75, 204
AspS9I GGNCC 3 cut(s) 661, 713, 889
AsuC2I CCSGG 1 cut(s) 253
AsuHPI GGTGA 2 cut(s) 455, 836
AvaII GGWCC 2 cut(s) 713, 889
BanI GGYRCC 3 cut(s) 72, 142, 448
BauI CACGAG 1 cut(s) 358
BbsI GAAGAC 1 cut(s) 962
BbvI GCAGC 3 cut(s) 94, 97, 318
BccI CCATC 1 cut(s) 958
BceAI ACGGC 2 cut(s) 67, 95
BcgI CGANNNNNNTGC 2 cut(s) 933, 967
BciT130I CCWGG 1 cut(s) 711
BclI TGATCA 1 cut(s) 748
BcnI CCSGG 1 cut(s) 253
BcoDI GTCTC 1 cut(s) 357
BfaI CTAG 1 cut(s) 90
BfmI CTRYAG 1 cut(s) 332
BfoI RGCGCY 2 cut(s) 76, 205
BglII AGATCT 1 cut(s) 778
BisI GCNGC 5 cut(s) 105, 108, 111, 332, 483
BlsI GCNGC 5 cut(s) 106, 109, 112, 333, 484
Bme1390I CCNGG 2 cut(s) 253, 711
Bme18I GGWCC 2 cut(s) 713, 889
BmgT120I GGNCC 3 cut(s) 661, 713, 889
BmiI GGNNCC 4 cut(s) 74, 144, 450, 891
BmrFI CCNGG 2 cut(s) 253, 711
BmsI GCATC 2 cut(s) 327, 931
BpiI GAAGAC 1 cut(s) 962
BpmI CTGGAG 1 cut(s) 693
Bpu10I CCTNAGC 1 cut(s) 894
BpuEI CTTGAG 1 cut(s) 522
BpuMI CCSGG 1 cut(s) 253
BsaBI GATNNNNATC 1 cut(s) 615
BsaHI GRCGYC 2 cut(s) 13, 73
BsaJI CCNNGG 3 cut(s) 114, 252, 500
BsaWI WCCGGW 1 cut(s) 440
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bsc4I CCNNNNNNNGG 1 cut(s) 821
Bse118I RCCGGY 1 cut(s) 440
Bse1I ACTGG 4 cut(s) 232, 383, 558, 924
Bse8I GATNNNNATC 1 cut(s) 615
BseBI CCWGG 1 cut(s) 711
BseDI CCNNGG 3 cut(s) 114, 252, 500
BseGI GGATG 2 cut(s) 318, 811
BseJI GATNNNNATC 1 cut(s) 615
BseLI CCNNNNNNNGG 1 cut(s) 821
BseMII CTCAG 4 cut(s) 293, 399, 690, 885
BseNI ACTGG 4 cut(s) 232, 383, 558, 924
BseRI GAGGAG 1 cut(s) 664
BseX3I CGGCCG 1 cut(s) 80
BseXI GCAGC 3 cut(s) 94, 97, 318
Bsh1236I CGCG 1 cut(s) 104
Bsh1285I CGRYCG 1 cut(s) 83
BshFI GGCC 2 cut(s) 82, 662
BshNI GGYRCC 3 cut(s) 72, 142, 448
BshTI ACCGGT 1 cut(s) 440
BsiEI CGRYCG 1 cut(s) 83
BsiSI CCGG 2 cut(s) 252, 441
BsiWI CGTACG 1 cut(s) 84
BslFI GGGAC 2 cut(s) 143, 875
BslI CCNNNNNNNGG 1 cut(s) 821
BsmAI GTCTC 1 cut(s) 357
BsmFI GGGAC 2 cut(s) 143, 875
BsmI GAATGC 2 cut(s) 253, 257
BsnI GGCC 2 cut(s) 82, 662
Bsp143I GATC 4 cut(s) 376, 616, 748, 778
BspACI CCGC 5 cut(s) 104, 137, 197, 482, 636
BspANI GGCC 2 cut(s) 82, 662
BspCNI CTCAG 4 cut(s) 292, 400, 689, 886
BspFNI CGCG 1 cut(s) 104
BspLI GGNNCC 4 cut(s) 74, 144, 450, 891
BspMAI CTGCAG 1 cut(s) 336
BspPI GGATC 1 cut(s) 384
BspQI GCTCTTC 1 cut(s) 308
BspT107I GGYRCC 3 cut(s) 72, 142, 448
BsrFI RCCGGY 1 cut(s) 440
BsrI ACTGG 4 cut(s) 232, 383, 558, 924
BssAI RCCGGY 1 cut(s) 440
BssECI CCNNGG 3 cut(s) 114, 252, 500
BssMI GATC 4 cut(s) 376, 616, 748, 778
BssNI GRCGYC 2 cut(s) 13, 73
BssSI CACGAG 1 cut(s) 358
BssT1I CCWWGG 1 cut(s) 500
Bst2BI CACGAG 1 cut(s) 358
Bst2UI CCWGG 1 cut(s) 711
Bst4CI ACNGT 2 cut(s) 268, 626
Bst6I CTCTTC 6 cut(s) 308, 423, 540, 651, 690, 713
BstACI GRCGYC 2 cut(s) 13, 73
BstAPI GCANNNNNTGC 1 cut(s) 248
BstC8I GCNNGC 2 cut(s) 453, 933
BstDEI CTNAG 6 cut(s) 279, 345, 408, 597, 676, 894
BstEII GGTNACC 1 cut(s) 700
BstF5I GGATG 2 cut(s) 318, 811
BstFNI CGCG 1 cut(s) 104
BstH2I RGCGCY 2 cut(s) 76, 205
BstHHI GCGC 2 cut(s) 75, 204
BstKTI GATC 4 cut(s) 379, 619, 751, 781
BstMAI GTCTC 1 cut(s) 357
BstMBI GATC 4 cut(s) 376, 616, 748, 778
BstMCI CGRYCG 1 cut(s) 83
BstMWI GCNNNNNNNGC 5 cut(s) 79, 110, 116, 248, 941
BstNI CCWGG 1 cut(s) 711
BstPI GGTNACC 1 cut(s) 700
BstSCI CCNGG 2 cut(s) 251, 709
BstSFI CTRYAG 1 cut(s) 332
BstUI CGCG 1 cut(s) 104
BstV1I GCAGC 3 cut(s) 94, 97, 318
BstV2I GAAGAC 1 cut(s) 962
BstX2I RGATCY 2 cut(s) 376, 778
BstYI RGATCY 2 cut(s) 376, 778
BstZI CGGCCG 1 cut(s) 80
BsuRI GGCC 2 cut(s) 82, 662
BtgZI GCGATG 1 cut(s) 500
BtsCI GGATG 2 cut(s) 318, 811
BtsIMutI CAGTG 2 cut(s) 565, 586
Cac8I GCNNGC 2 cut(s) 453, 933
CaiI CAGNNNCTG 1 cut(s) 331
CfoI GCGC 2 cut(s) 75, 204
Cfr10I RCCGGY 1 cut(s) 440
Cfr13I GGNCC 3 cut(s) 661, 713, 889
CseI GACGC 1 cut(s) 21
Csp6I GTAC 5 cut(s) 85, 235, 337, 386, 927
CspAI ACCGGT 1 cut(s) 440
CviAII CATG 2 cut(s) 422, 755
CviQI GTAC 5 cut(s) 85, 235, 337, 386, 927
DdeI CTNAG 6 cut(s) 279, 345, 408, 597, 676, 894
DinI GGCGCC 1 cut(s) 74
DpnI GATC 4 cut(s) 378, 618, 750, 780
DpnII GATC 4 cut(s) 376, 616, 748, 778
EaeI YGGCCR 1 cut(s) 80
EagI CGGCCG 1 cut(s) 80
Eam1104I CTCTTC 6 cut(s) 308, 423, 540, 651, 690, 713
EarI CTCTTC 6 cut(s) 308, 423, 540, 651, 690, 713
EciI GGCGGA 1 cut(s) 152
EclXI CGGCCG 1 cut(s) 80
Eco130I CCWWGG 1 cut(s) 500
Eco47I GGWCC 2 cut(s) 713, 889
Eco52I CGGCCG 1 cut(s) 80
Eco57I CTGAAG 3 cut(s) 308, 714, 957
Eco91I GGTNACC 1 cut(s) 700
EcoO109I RGGNCCY 1 cut(s) 889
EcoO65I GGTNACC 1 cut(s) 700
EcoRI GAATTC 1 cut(s) 550
EcoRII CCWGG 1 cut(s) 709
EcoT14I CCWWGG 1 cut(s) 500
EgeI GGCGCC 1 cut(s) 74
EheI GGCGCC 1 cut(s) 74
ErhI CCWWGG 1 cut(s) 500
FaeI CATG 2 cut(s) 425, 758
FalI AAGNNNNNCTT 2 cut(s) 198, 230
FaqI GGGAC 2 cut(s) 143, 875
FatI CATG 2 cut(s) 421, 754
FauI CCCGC 1 cut(s) 629
FauNDI CATATG 2 cut(s) 274, 607
FbaI TGATCA 1 cut(s) 748
Fnu4HI GCNGC 5 cut(s) 105, 108, 111, 332, 483
FokI GGATG 2 cut(s) 305, 798
Fsp4HI GCNGC 5 cut(s) 105, 108, 111, 332, 483
FspBI CTAG 1 cut(s) 90
GlaI GCGC 2 cut(s) 74, 203
GluI GCNGC 5 cut(s) 105, 108, 111, 332, 483
GsuI CTGGAG 1 cut(s) 693
HaeII RGCGCY 2 cut(s) 76, 205
HaeIII GGCC 2 cut(s) 82, 662
HapII CCGG 2 cut(s) 252, 441
HgaI GACGC 1 cut(s) 21
HhaI GCGC 2 cut(s) 75, 204
Hin1I GRCGYC 2 cut(s) 13, 73
Hin1II CATG 2 cut(s) 425, 758
Hin6I GCGC 2 cut(s) 73, 202
HinP1I GCGC 2 cut(s) 73, 202
HincII GTYRAC 2 cut(s) 229, 682
HindII GTYRAC 2 cut(s) 229, 682
HinfI GANTC 6 cut(s) 218, 418, 458, 535, 593, 857
HpaI GTTAAC 1 cut(s) 682
HpaII CCGG 2 cut(s) 252, 441
HphI GGTGA 2 cut(s) 455, 836
Hpy166II GTNNAC 2 cut(s) 229, 682
Hpy188I TCNGA 3 cut(s) 282, 694, 1016
Hpy188III TCNNGA 2 cut(s) 310, 326
Hpy8I GTNNAC 2 cut(s) 229, 682
Hpy99I CGWCG 3 cut(s) 12, 15, 81
HpyAV CCTTC 3 cut(s) 40, 332, 506
HpyCH4III ACNGT 2 cut(s) 268, 626
HpyCH4IV ACGT 1 cut(s) 684
HpyCH4V TGCA 4 cut(s) 334, 629, 767, 944
HpyF10VI GCNNNNNNNGC 5 cut(s) 79, 110, 116, 248, 941
HpyF3I CTNAG 6 cut(s) 279, 345, 408, 597, 676, 894
HpySE526I ACGT 1 cut(s) 684
Hsp92I GRCGYC 2 cut(s) 13, 73
Hsp92II CATG 2 cut(s) 425, 758
HspAI GCGC 2 cut(s) 73, 202
KasI GGCGCC 1 cut(s) 72
Ksp22I TGATCA 1 cut(s) 748
KspAI GTTAAC 1 cut(s) 682
Kzo9I GATC 4 cut(s) 376, 616, 748, 778
LguI GCTCTTC 1 cut(s) 308
LmnI GCTCC 2 cut(s) 199, 328
Lsp1109I GCAGC 3 cut(s) 94, 97, 318
LweI GCATC 2 cut(s) 327, 931
MaeI CTAG 1 cut(s) 90
MaeII ACGT 1 cut(s) 684
MaeIII GTNAC 4 cut(s) 3, 92, 532, 700
MalI GATC 4 cut(s) 378, 618, 750, 780
MboI GATC 4 cut(s) 376, 616, 748, 778
MboII GAAGA 9 cut(s) 325, 440, 521, 557, 668, 707, 730, 950, 967
MflI RGATCY 2 cut(s) 376, 778
MluCI AATT 6 cut(s) 287, 522, 550, 588, 640, 758
Mly113I GGCGCC 1 cut(s) 73
MlyI GAGTC 3 cut(s) 412, 529, 851
MmeI TCCRAC 1 cut(s) 63
MseI TTAA 4 cut(s) 174, 525, 621, 681
MspA1I CMGCKG 2 cut(s) 455, 572
MspI CCGG 2 cut(s) 252, 441
MspR9I CCNGG 2 cut(s) 253, 711
Mva1269I GAATGC 2 cut(s) 253, 257
MvaI CCWGG 1 cut(s) 711
MvnI CGCG 1 cut(s) 104
MwoI GCNNNNNNNGC 5 cut(s) 79, 110, 116, 248, 941
NarI GGCGCC 1 cut(s) 73
NciI CCSGG 1 cut(s) 253
NdeI CATATG 2 cut(s) 274, 607
NdeII GATC 4 cut(s) 376, 616, 748, 778
NlaIII CATG 2 cut(s) 425, 758
NlaIV GGNNCC 4 cut(s) 74, 144, 450, 891
NmeAIII GCCGAG 1 cut(s) 139
NmuCI GTSAC 1 cut(s) 532
PciSI GCTCTTC 1 cut(s) 308
PctI GAATGC 2 cut(s) 253, 257
PfeI GAWTC 3 cut(s) 218, 458, 593
Pfl23II CGTACG 1 cut(s) 84
PfoI TCCNGGA 1 cut(s) 709
PinAI ACCGGT 1 cut(s) 440
PkrI GCNGC 5 cut(s) 106, 109, 112, 333, 484
PleI GAGTC 3 cut(s) 412, 529, 851
PluTI GGCGCC 1 cut(s) 76
PpsI GAGTC 3 cut(s) 412, 529, 851
PpuMI RGGWCCY 1 cut(s) 889
Psp1406I AACGTT 1 cut(s) 684
Psp5II RGGWCCY 1 cut(s) 889
Psp6I CCWGG 1 cut(s) 709
PspEI GGTNACC 1 cut(s) 700
PspGI CCWGG 1 cut(s) 709
PspLI CGTACG 1 cut(s) 84
PspN4I GGNNCC 4 cut(s) 74, 144, 450, 891
PspPI GGNCC 3 cut(s) 661, 713, 889
PspPPI RGGWCCY 1 cut(s) 889
PstI CTGCAG 1 cut(s) 336
PstNI CAGNNNCTG 1 cut(s) 331
PsuI RGATCY 2 cut(s) 376, 778
PvuII CAGCTG 2 cut(s) 455, 572
RsaI GTAC 5 cut(s) 86, 236, 338, 387, 928
RsaNI GTAC 5 cut(s) 85, 235, 337, 386, 927
SapI GCTCTTC 1 cut(s) 308
SaqAI TTAA 4 cut(s) 174, 525, 621, 681
SatI GCNGC 5 cut(s) 105, 108, 111, 332, 483
Sau3AI GATC 4 cut(s) 376, 616, 748, 778
Sau96I GGNCC 3 cut(s) 661, 713, 889
SchI GAGTC 3 cut(s) 412, 529, 851
ScrFI CCNGG 2 cut(s) 253, 711
SfaNI GCATC 2 cut(s) 327, 931
SfcI CTRYAG 1 cut(s) 332
SfoI GGCGCC 1 cut(s) 74
SinI GGWCC 2 cut(s) 713, 889
SmlI CTYRAG 1 cut(s) 537
SmoI CTYRAG 1 cut(s) 537
Sse9I AATT 6 cut(s) 287, 522, 550, 588, 640, 758
SsiI CCGC 5 cut(s) 104, 137, 197, 482, 636
SspDI GGCGCC 1 cut(s) 72
SspMI CTAG 1 cut(s) 90
StyD4I CCNGG 2 cut(s) 251, 709
StyI CCWWGG 1 cut(s) 500
TaaI ACNGT 2 cut(s) 268, 626
TaiI ACGT 1 cut(s) 687
TaqI TCGA 1 cut(s) 953
TaqII GACCGA 1 cut(s) 730
TasI AATT 6 cut(s) 287, 522, 550, 588, 640, 758
TatI WGTACW 3 cut(s) 234, 336, 385
TauI GCSGC 2 cut(s) 107, 485
TfiI GAWTC 3 cut(s) 218, 458, 593
Tru1I TTAA 4 cut(s) 174, 525, 621, 681
Tru9I TTAA 4 cut(s) 174, 525, 621, 681
TscAI CASTG 2 cut(s) 565, 586
TseFI GTSAC 1 cut(s) 532
TseI GCWGC 3 cut(s) 107, 110, 331
Tsp45I GTSAC 1 cut(s) 532
TspDTI ATGAA 3 cut(s) 261, 771, 995
TspRI CASTG 2 cut(s) 565, 586
VpaK11BI GGWCC 2 cut(s) 713, 889
XapI RAATTY 3 cut(s) 550, 640, 758
XspI CTAG 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.