Rh4BG100100

Hydroxyproline-rich glycoprotein family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0006684
Physical Location & Seq
Forward (+)
17200779 .. 17201563
785 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG100100.1

Sequence Viewer

Length: 504 bp
ATGGTAGGAGCACTTGGGTCAGATGTCGCCCTCGTGTGGTGGAATCGGGTCGCCGGTAGGAGCACTCGGGTCAACGATCCGCCGCGGCAGCGTCCGTCTTCTGCAGCTGAGCAGGAATCTCTCTGTCGCAGGCCAGCTTTAGCGACTCGGCCCTATGCTAACAATGCCGCCGCCGCCGAGGAACCAAACCCAGATGGCAACAACAAAGTCCGTGGAAGGAGGCTTAGCAACAAGGAGCTCAAAGCCCTTGTGGAATCATTTGTCCACGAGTACAGGGCAATGAATGCGGGGAAATTCCCTCCCTTTAAGTATACTCAGAGACAAGTGGGTGGCAGTTACTATAGAATTAGGGGGATCCTCCAGGAGCTACAGTTCACGGCTAAGATGTCGCCCTCGTGTGGTGGAATCGGGTCGCCGGTAGGAGCACTCAAGTCAACGATCCGCCGCGGCAGCGTCCGTCTTCTACAGCTGAGCAGGAATCTCTCTGTCGCAGGCCAGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

167

Amino Acids

18.3

Weight (kDa)

10.89

Isoelectric Point (pI)

62.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HTH_AT3G52170 PF25896 77 - 124 3.4e-22 Helix-turn-helix domain in AT3G52170-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 311
AccII CGCG 2 cut(s) 85, 447
AclWI GGATC 4 cut(s) 71, 349, 362, 433
AcsI RAATTY 1 cut(s) 293
AfaI GTAC 1 cut(s) 272
AfiI CCNNNNNNNGG 2 cut(s) 36, 398
AjnI CCWGG 1 cut(s) 360
AloI GAACNNNNNNTCC 2 cut(s) 356, 388
AluBI AGCT 6 cut(s) 107, 137, 238, 367, 469, 499
AluI AGCT 6 cut(s) 107, 137, 238, 367, 469, 499
Alw21I GWGCWC 4 cut(s) 13, 65, 240, 427
Alw26I GTCTC 1 cut(s) 313
AlwI GGATC 4 cut(s) 71, 349, 362, 433
Ama87I CYCGRG 1 cut(s) 66
AoxI GGCC 3 cut(s) 131, 149, 493
ApeKI GCWGC 3 cut(s) 88, 104, 450
ApoI RAATTY 1 cut(s) 293
AspS9I GGNCC 1 cut(s) 150
AvaI CYCGRG 1 cut(s) 66
BamHI GGATCC 1 cut(s) 354
BanII GRGCYC 1 cut(s) 240
BauI CACGAG 3 cut(s) 32, 266, 394
BbsI GAAGAC 2 cut(s) 90, 452
Bbv12I GWGCWC 4 cut(s) 13, 65, 240, 427
BbvI GCAGC 3 cut(s) 100, 116, 462
BccI CCATC 1 cut(s) 188
BceAI ACGGC 1 cut(s) 393
BciT130I CCWGG 1 cut(s) 362
BcoDI GTCTC 1 cut(s) 313
BfmI CTRYAG 4 cut(s) 102, 340, 368, 464
BlpI GCTNAGC 3 cut(s) 108, 224, 470
Bme1390I CCNGG 1 cut(s) 362
BmeT110I CYCGRG 1 cut(s) 66
BmgT120I GGNCC 1 cut(s) 150
BmiI GGNNCC 2 cut(s) 183, 356
BmrFI CCNGG 1 cut(s) 362
BpiI GAAGAC 2 cut(s) 90, 452
BpmI CTGGAG 1 cut(s) 344
Bpu1102I GCTNAGC 3 cut(s) 108, 224, 470
BpuEI CTTGAG 1 cut(s) 413
BsaJI CCNNGG 4 cut(s) 83, 177, 211, 445
BsaXI ACNNNNNCTCC 2 cut(s) 356, 386
Bsc4I CCNNNNNNNGG 2 cut(s) 36, 398
Bse118I RCCGGY 2 cut(s) 53, 415
Bse3DI GCAATG 1 cut(s) 285
BseBI CCWGG 1 cut(s) 362
BseDI CCNNGG 4 cut(s) 83, 177, 211, 445
BseLI CCNNNNNNNGG 2 cut(s) 36, 398
BseMI GCAATG 1 cut(s) 285
BseMII CTCAG 3 cut(s) 99, 329, 461
BseXI GCAGC 3 cut(s) 100, 116, 462
Bsh1236I CGCG 2 cut(s) 85, 447
BshFI GGCC 3 cut(s) 133, 151, 495
BsiHKAI GWGCWC 4 cut(s) 13, 65, 240, 427
BsiHKCI CYCGRG 1 cut(s) 66
BsiSI CCGG 2 cut(s) 54, 416
BslI CCNNNNNNNGG 2 cut(s) 36, 398
BsmAI GTCTC 1 cut(s) 313
BsmI GAATGC 1 cut(s) 289
BsnI GGCC 3 cut(s) 133, 151, 495
BsoBI CYCGRG 1 cut(s) 66
Bsp1286I GDGCHC 4 cut(s) 13, 65, 240, 427
Bsp143I GATC 3 cut(s) 76, 354, 438
Bsp1720I GCTNAGC 3 cut(s) 108, 224, 470
BspANI GGCC 3 cut(s) 133, 151, 495
BspCNI CTCAG 3 cut(s) 100, 328, 462
BspFNI CGCG 2 cut(s) 85, 447
BspLI GGNNCC 2 cut(s) 183, 356
BspMAI CTGCAG 1 cut(s) 106
BspPI GGATC 4 cut(s) 71, 349, 362, 433
BsrDI GCAATG 1 cut(s) 285
BsrFI RCCGGY 2 cut(s) 53, 415
BssAI RCCGGY 2 cut(s) 53, 415
BssECI CCNNGG 4 cut(s) 83, 177, 211, 445
BssMI GATC 3 cut(s) 76, 354, 438
BssNAI GTATAC 1 cut(s) 312
BssSI CACGAG 3 cut(s) 32, 266, 394
Bst1107I GTATAC 1 cut(s) 312
Bst2BI CACGAG 3 cut(s) 32, 266, 394
Bst2UI CCWGG 1 cut(s) 362
Bst4CI ACNGT 1 cut(s) 372
BstAPI GCANNNNNTGC 1 cut(s) 284
BstC8I GCNNGC 4 cut(s) 131, 135, 493, 497
BstDEI CTNAG 5 cut(s) 108, 224, 315, 381, 470
BstDSI CCRYGG 3 cut(s) 83, 211, 445
BstFNI CGCG 2 cut(s) 85, 447
BstKTI GATC 3 cut(s) 79, 357, 441
BstMAI GTCTC 1 cut(s) 313
BstMBI GATC 3 cut(s) 76, 354, 438
BstMWI GCNNNNNNNGC 5 cut(s) 88, 164, 173, 284, 450
BstNI CCWGG 1 cut(s) 362
BstSCI CCNGG 1 cut(s) 360
BstSFI CTRYAG 4 cut(s) 102, 340, 368, 464
BstUI CGCG 2 cut(s) 85, 447
BstV1I GCAGC 3 cut(s) 100, 116, 462
BstV2I GAAGAC 2 cut(s) 90, 452
BstX2I RGATCY 1 cut(s) 354
BstYI RGATCY 1 cut(s) 354
BstZ17I GTATAC 1 cut(s) 312
BsuRI GGCC 3 cut(s) 133, 151, 495
BtgI CCRYGG 3 cut(s) 83, 211, 445
Cac8I GCNNGC 4 cut(s) 131, 135, 493, 497
Cfr10I RCCGGY 2 cut(s) 53, 415
Cfr13I GGNCC 1 cut(s) 150
Cfr42I CCGCGG 2 cut(s) 86, 448
CseI GACGC 2 cut(s) 80, 442
Csp6I GTAC 1 cut(s) 271
CspCI CAANNNNNGTGG 2 cut(s) 193, 228
CviQI GTAC 1 cut(s) 271
DdeI CTNAG 5 cut(s) 108, 224, 315, 381, 470
DpnI GATC 3 cut(s) 78, 356, 440
DpnII GATC 3 cut(s) 76, 354, 438
EciI GGCGGA 2 cut(s) 69, 431
Ecl136II GAGCTC 1 cut(s) 238
Eco24I GRGCYC 1 cut(s) 240
Eco53kI GAGCTC 1 cut(s) 238
Eco88I CYCGRG 1 cut(s) 66
EcoICRI GAGCTC 1 cut(s) 238
EcoRII CCWGG 1 cut(s) 360
EcoT38I GRGCYC 1 cut(s) 240
FaiI YATR 3 cut(s) 156, 312, 342
FauI CCCGC 1 cut(s) 280
FblI GTMKAC 1 cut(s) 311
FriOI GRGCYC 1 cut(s) 240
GsuI CTGGAG 1 cut(s) 344
HaeIII GGCC 3 cut(s) 133, 151, 495
HapII CCGG 2 cut(s) 54, 416
HgaI GACGC 2 cut(s) 80, 442
HincII GTYRAC 2 cut(s) 73, 435
HindII GTYRAC 2 cut(s) 73, 435
HinfI GANTC 6 cut(s) 43, 116, 145, 254, 405, 478
HpaII CCGG 2 cut(s) 54, 416
Hpy166II GTNNAC 5 cut(s) 73, 265, 312, 375, 435
Hpy188I TCNGA 2 cut(s) 22, 318
Hpy8I GTNNAC 5 cut(s) 73, 265, 312, 375, 435
HpyAV CCTTC 1 cut(s) 210
HpyCH4III ACNGT 1 cut(s) 372
HpyCH4V TGCA 1 cut(s) 104
HpyF10VI GCNNNNNNNGC 5 cut(s) 88, 164, 173, 284, 450
HpyF3I CTNAG 5 cut(s) 108, 224, 315, 381, 470
KspI CCGCGG 2 cut(s) 86, 448
Kzo9I GATC 3 cut(s) 76, 354, 438
LmnI GCTCC 5 cut(s) 8, 60, 235, 364, 422
Lsp1109I GCAGC 3 cut(s) 100, 116, 462
MaeIII GTNAC 1 cut(s) 335
MalI GATC 3 cut(s) 78, 356, 440
MboI GATC 3 cut(s) 76, 354, 438
MboII GAAGA 2 cut(s) 90, 452
MflI RGATCY 1 cut(s) 354
MhlI GDGCHC 4 cut(s) 13, 65, 240, 427
MluCI AATT 2 cut(s) 293, 345
MlyI GAGTC 1 cut(s) 139
MnlI CCTC 6 cut(s) 41, 172, 213, 309, 368, 403
MseI TTAA 1 cut(s) 306
MspA1I CMGCKG 4 cut(s) 85, 107, 447, 469
MspI CCGG 2 cut(s) 54, 416
MspR9I CCNGG 1 cut(s) 362
Mva1269I GAATGC 1 cut(s) 289
MvaI CCWGG 1 cut(s) 362
MvnI CGCG 2 cut(s) 85, 447
MwoI GCNNNNNNNGC 5 cut(s) 88, 164, 173, 284, 450
NdeII GATC 3 cut(s) 76, 354, 438
NlaIV GGNNCC 2 cut(s) 183, 356
NmeAIII GCCGAG 2 cut(s) 127, 202
PctI GAATGC 1 cut(s) 289
PfeI GAWTC 5 cut(s) 43, 116, 254, 405, 478
PfoI TCCNGGA 1 cut(s) 360
PleI GAGTC 1 cut(s) 139
PpsI GAGTC 1 cut(s) 139
Psp124BI GAGCTC 1 cut(s) 240
Psp6I CCWGG 1 cut(s) 360
PspGI CCWGG 1 cut(s) 360
PspN4I GGNNCC 2 cut(s) 183, 356
PspPI GGNCC 1 cut(s) 150
PstI CTGCAG 1 cut(s) 106
PsuI RGATCY 1 cut(s) 354
PvuII CAGCTG 2 cut(s) 107, 469
RsaI GTAC 1 cut(s) 272
RsaNI GTAC 1 cut(s) 271
SacI GAGCTC 1 cut(s) 240
SacII CCGCGG 2 cut(s) 86, 448
SaqAI TTAA 1 cut(s) 306
Sau3AI GATC 3 cut(s) 76, 354, 438
Sau96I GGNCC 1 cut(s) 150
SchI GAGTC 1 cut(s) 139
ScrFI CCNGG 1 cut(s) 362
SduI GDGCHC 4 cut(s) 13, 65, 240, 427
SetI ASST 6 cut(s) 109, 139, 240, 369, 471, 501
SfcI CTRYAG 4 cut(s) 102, 340, 368, 464
Sfr303I CCGCGG 2 cut(s) 86, 448
SgrBI CCGCGG 2 cut(s) 86, 448
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
Sse9I AATT 2 cut(s) 293, 345
SstI GAGCTC 1 cut(s) 240
StyD4I CCNGG 1 cut(s) 360
TaaI ACNGT 1 cut(s) 372
TasI AATT 2 cut(s) 293, 345
TatI WGTACW 1 cut(s) 270
TauI GCSGC 7 cut(s) 85, 88, 170, 173, 176, 447, 450
TfiI GAWTC 5 cut(s) 43, 116, 254, 405, 478
Tru1I TTAA 1 cut(s) 306
Tru9I TTAA 1 cut(s) 306
TseI GCWGC 3 cut(s) 88, 104, 450
TspDTI ATGAA 1 cut(s) 296
TspGWI ACGGA 3 cut(s) 84, 200, 446
XapI RAATTY 1 cut(s) 293
XmiI GTMKAC 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.