Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0006729

Physical Location & Seq

Genomic Coordinates Reverse (-)

10894543 .. 10899191

Gene Structure 4649 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1076700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1320 bp

ATGGCAGTTCTCGGAGAGACAACCGAAGCCTCAAACAACTCCCTCTCCCACAAGATCACCGCCAAAACCCCATTATTCAACCTCAAACTCTACGCTGTTATCGCCATCCTCGTCCTCTGCCTCCTCGCAGCCGCCCTTCTCATCTTCCTCTGCCTCCGCATAACTCGAGTCTCCCGAAAACGCAAGATGCGCGTGAAGCACAGCTCCGGCTCAATCCCCCTAGTCTCCAAGGAGATCGCCGAGATCAAGGAACCGAGTCCACGCGCCGCCGACAATGGCGAGAGAAAAATGGGAAATGAAAAGTTGAAGGAAGCTCCGCTGGAGGTGACGGAGATTGTTGACATCGAAAGAGGGAAAGGGAACCGGAGCGGGTTGGAAAGTGACGGGTCGGGCCCGCATAACATCGGGTGGGGCCGGTGGTACAGCTTGAAGGAGCTGGAGATCGCGACACGCGGGTTTTCTCCACAGAACGTGATTGGAGAAGGAGGATACGGCATCGTCTACAGCGGCGAGCTGCAGGACGGCTCTGTCGTGGCCGTCAAGAACCTTCTGAACAACAAGGGTCAGGCAGAGAACGAGTTTAAGGTGGAAGTCGAAGCCATTGGGAAAGTAAAGCATAAGAACTTGGTGGGTCTAATTGGTTATTGTGCAGAAGGTGCTCAAAGGATGCTTGTGTACGAGTACATTGACAACGGCAACTTGGAGCAATGGTTGCACGGCGATGTTGGGCCTGTCAGCCCTCTGACCTGGGATATTCGTATGAAAATCGCCATCGGGACAGCAAAAGGGCTGGCCTATTTGCATGAAGGGTTAGAACCCAAGGTGGTGCACCGTGATATAAAATCCAGTAACATTCTTCTGGATAGAAAGTGGAACGCAAAAGTATCAGATTTTGGTCTGGCCAAGCTCTTAGGATCGGAGGCAAGCTATGTGACTACACGCGTTATGGGGACATTCGGATATGTAGCTCCAGAATATGCAAGCACAGGTATGCTTAACGAGGGGAGTGATGTATATAGTTTCGGAGTTCTACTAATGGAGATAATTACAGGAAGAAACCCAATTGACTATTCCAAACCAGCAGGAGAGATGAACCTGGTTGATTGGTTTAAAGGAATGATCCAAAGTCGCCGTGGAGAGGATGTTGTTGATCCTCTGATTGCAGTTCAGCCCCCTCCAAGAGCTATGAAGCGAGTATTGCTGGTTTGTCTCCGATGTATAGATCTAGATGTCCATAAGCGACCGAAAATGGGGCAAATTGTTCATATGCTTGAGGCAGATGACTTCCCTTTTCGTTCAGAAACTCGATCAGCTCGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

440

Amino Acids

48.46

Weight (kDa)

8.73

Isoelectric Point (pI)

35.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	156 - 424	1.7e-47	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	156 - 422	6e-45	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006729)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G34500
fragaria_vesca	FvH4_2g08720 FvH4_2g08720 FvH4_2g08720
malus_domestica	MD05G1067100.v1.1 MD10G1076700.v1.1
prunus_persica	Prupe.8G097500_v2.0.a1
pyrus_communis	pycom05g05790 pycom10g06000
rosa_chinensis	RchiOBHm_Chr6g0264191
rosa_laevigata	RLG00000014281
rosa_multiflora	Rmu_sc0005325.1_g000012 Rmu_sc0007214.1_g000014
rosa_roxburghii	Rroxscaffold_176G00431530 Rroxscaffold_176G00431580 Rroxscaffold_4G00299000 Rroxscaffold_7G00203440
rosa_rugosa	Rorug06G0009700 Rorug06G0009800
rosa_samantha	Rh6AG130100 Rh6BG125900 Rh6BG126900 Rh6BG127300 Rh6CG125800 Rh6DG112600
rosa_wichuraiana	Rw6G011320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	527
AccBSI	CCGCTC	1 cut(s)	369
AccI	GTMKAC	1 cut(s)	501
AccII	CGCG	5 cut(s)	192, 264, 446, 453, 942
AciI	CCGC	9 cut(s)	60, 132, 157, 267, 317, 369, 395, 453, 507
AclWI	GGATC	3 cut(s)	922, 1114, 1145
AcoI	YGGCCR	2 cut(s)	534, 900
AfaI	GTAC	3 cut(s)	422, 677, 683
AfiI	CCNNNNNNNGG	2 cut(s)	1138, 1250
AflIII	ACRYGT	1 cut(s)	940
AgsI	TTSAA	3 cut(s)	79, 307, 430
AjnI	CCWGG	2 cut(s)	746, 1095
AjuI	GAANNNNNNNTTGG	2 cut(s)	1054, 1086
Alw21I	GWGCWC	2 cut(s)	661, 831
Alw26I	GTCTC	4 cut(s)	11, 175, 229, 1214
Alw44I	GTGCAC	1 cut(s)	827
AlwI	GGATC	3 cut(s)	922, 1114, 1145
Ama87I	CYCGRG	1 cut(s)	165
AoxI	GGCC	6 cut(s)	391, 412, 534, 728, 792, 900
ApaI	GGGCCC	1 cut(s)	395
ApaLI	GTGCAC	1 cut(s)	827
ApeKI	GCWGC	2 cut(s)	128, 514
AspLEI	GCGC	2 cut(s)	192, 266
AspS9I	GGNCC	4 cut(s)	391, 392, 412, 728
AsuHPI	GGTGA	2 cut(s)	49, 337
AvaI	CYCGRG	1 cut(s)	165
BaeGI	GKGCMC	2 cut(s)	395, 831
BalI	TGGCCA	1 cut(s)	902
BanII	GRGCYC	1 cut(s)	395
Bbv12I	GWGCWC	2 cut(s)	661, 831
BbvI	GCAGC	2 cut(s)	140, 501
BccI	CCATC	2 cut(s)	113, 779
BceAI	ACGGC	6 cut(s)	508, 521, 538, 709, 733, 1116
BciT130I	CCWGG	2 cut(s)	748, 1097
BciVI	GTATCC	1 cut(s)	482
BcoDI	GTCTC	4 cut(s)	11, 175, 229, 1214
BfaI	CTAG	2 cut(s)	221, 1226
BfmI	CTRYAG	2 cut(s)	502, 515
BfuI	GTATCC	1 cut(s)	482
BglII	AGATCT	1 cut(s)	1222
BisI	GCNGC	5 cut(s)	129, 132, 267, 508, 515
BlsI	GCNGC	5 cut(s)	130, 133, 268, 509, 516
Bme1390I	CCNGG	2 cut(s)	748, 1097
BmeT110I	CYCGRG	1 cut(s)	165
BmgT120I	GGNCC	4 cut(s)	391, 392, 412, 728
BmiI	GGNNCC	4 cut(s)	252, 362, 393, 413
BmrFI	CCNGG	2 cut(s)	748, 1097
BmsI	GCATC	3 cut(s)	177, 504, 657
BpmI	CTGGAG	3 cut(s)	341, 458, 954
BpuEI	CTTGAG	1 cut(s)	1292
BsaJI	CCNNGG	4 cut(s)	228, 747, 819, 1132
BsaWI	WCCGGW	1 cut(s)	363
BsaXI	ACNNNNNCTCC	6 cut(s)	29, 59, 695, 725, 1128, 1158
Bsc4I	CCNNNNNNNGG	2 cut(s)	1138, 1250
Bse118I	RCCGGY	1 cut(s)	414
Bse1I	ACTGG	1 cut(s)	846
Bse3DI	GCAATG	1 cut(s)	713
BseBI	CCWGG	2 cut(s)	748, 1097
BseDI	CCNNGG	4 cut(s)	228, 747, 819, 1132
BseGI	GGATG	3 cut(s)	105, 672, 1147
BseLI	CCNNNNNNNGG	2 cut(s)	1138, 1250
BseMI	GCAATG	1 cut(s)	713
BseNI	ACTGG	1 cut(s)	846
BseRI	GAGGAG	1 cut(s)	113
BseSI	GKGCMC	2 cut(s)	395, 831
BseXI	GCAGC	2 cut(s)	140, 501
BsgI	GTGCAG	1 cut(s)	669
Bsh1236I	CGCG	5 cut(s)	192, 264, 446, 453, 942
Bsh1285I	CGRYCG	1 cut(s)	1244
BshFI	GGCC	6 cut(s)	393, 414, 536, 730, 794, 902
BsiEI	CGRYCG	1 cut(s)	1244
BsiHKAI	GWGCWC	2 cut(s)	661, 831
BsiHKCI	CYCGRG	1 cut(s)	165
BsiSI	CCGG	3 cut(s)	207, 364, 415
BslFI	GGGAC	2 cut(s)	790, 964
BslI	CCNNNNNNNGG	2 cut(s)	1138, 1250
BsmAI	GTCTC	4 cut(s)	11, 175, 229, 1214
BsmFI	GGGAC	2 cut(s)	790, 964
BsnI	GGCC	6 cut(s)	393, 414, 536, 730, 794, 902
BsoBI	CYCGRG	1 cut(s)	165
Bsp120I	GGGCCC	1 cut(s)	391
Bsp1286I	GDGCHC	3 cut(s)	395, 661, 831
Bsp143I	GATC	9 cut(s)	54, 234, 243, 441, 914, 1119, 1150, 1222, 1307
Bsp68I	TCGCGA	1 cut(s)	446
BspACI	CCGC	9 cut(s)	60, 132, 157, 267, 317, 369, 395, 453, 507
BspANI	GGCC	6 cut(s)	393, 414, 536, 730, 794, 902
BspFNI	CGCG	5 cut(s)	192, 264, 446, 453, 942
BspLI	GGNNCC	4 cut(s)	252, 362, 393, 413
BspMAI	CTGCAG	1 cut(s)	519
BspPI	GGATC	3 cut(s)	922, 1114, 1145
BsrBI	CCGCTC	1 cut(s)	369
BsrDI	GCAATG	1 cut(s)	713
BsrFI	RCCGGY	1 cut(s)	414
BsrI	ACTGG	1 cut(s)	846
BssAI	RCCGGY	1 cut(s)	414
BssECI	CCNNGG	4 cut(s)	228, 747, 819, 1132
BssMI	GATC	9 cut(s)	54, 234, 243, 441, 914, 1119, 1150, 1222, 1307
BssT1I	CCWWGG	2 cut(s)	228, 819
Bst2UI	CCWGG	2 cut(s)	748, 1097
Bst4CI	ACNGT	1 cut(s)	833
BstAPI	GCANNNNNTGC	2 cut(s)	656, 712
BstC8I	GCNNGC	5 cut(s)	395, 512, 792, 925, 982
BstDEI	CTNAG	1 cut(s)	910
BstDSI	CCRYGG	1 cut(s)	1132
BstF5I	GGATG	3 cut(s)	105, 672, 1147
BstFNI	CGCG	5 cut(s)	192, 264, 446, 453, 942
BstHHI	GCGC	2 cut(s)	192, 266
BstKTI	GATC	9 cut(s)	57, 237, 246, 444, 917, 1122, 1153, 1225, 1310
BstMAI	GTCTC	4 cut(s)	11, 175, 229, 1214
BstMBI	GATC	9 cut(s)	54, 234, 243, 441, 914, 1119, 1150, 1222, 1307
BstMCI	CGRYCG	1 cut(s)	1244
BstMWI	GCNNNNNNNGC	6 cut(s)	101, 189, 196, 656, 712, 1198
BstNI	CCWGG	2 cut(s)	748, 1097
BstSCI	CCNGG	2 cut(s)	746, 1095
BstSFI	CTRYAG	2 cut(s)	502, 515
BstSLI	GKGCMC	2 cut(s)	395, 831
BstUI	CGCG	5 cut(s)	192, 264, 446, 453, 942
BstV1I	GCAGC	2 cut(s)	140, 501
BstX2I	RGATCY	1 cut(s)	1222
BstYI	RGATCY	1 cut(s)	1222
BsuI	GTATCC	1 cut(s)	482
BsuRI	GGCC	6 cut(s)	393, 414, 536, 730, 794, 902
BtgI	CCRYGG	1 cut(s)	1132
BtgZI	GCGATG	1 cut(s)	735
BtsCI	GGATG	3 cut(s)	105, 672, 1147
BtuMI	TCGCGA	1 cut(s)	446
Cac8I	GCNNGC	5 cut(s)	395, 512, 792, 925, 982
CfoI	GCGC	2 cut(s)	192, 266
Cfr10I	RCCGGY	1 cut(s)	414
Cfr13I	GGNCC	4 cut(s)	391, 392, 412, 728
CsiI	ACCWGGT	1 cut(s)	1095
Csp6I	GTAC	3 cut(s)	421, 676, 682
CviAII	CATG	1 cut(s)	803
CviQI	GTAC	3 cut(s)	421, 676, 682
DdeI	CTNAG	1 cut(s)	910
DpnI	GATC	9 cut(s)	56, 236, 245, 443, 916, 1121, 1152, 1224, 1309
DpnII	GATC	9 cut(s)	54, 234, 243, 441, 914, 1119, 1150, 1222, 1307
DraI	TTTAAA	1 cut(s)	1111
DrdI	GACNNNNNNGTC	1 cut(s)	527
DseDI	GACNNNNNNGTC	1 cut(s)	527
EaeI	YGGCCR	2 cut(s)	534, 900
Eco130I	CCWWGG	2 cut(s)	228, 819
Eco24I	GRGCYC	1 cut(s)	395
Eco88I	CYCGRG	1 cut(s)	165
EcoRII	CCWGG	2 cut(s)	746, 1095
EcoT14I	CCWWGG	2 cut(s)	228, 819
EcoT38I	GRGCYC	1 cut(s)	395
ErhI	CCWWGG	2 cut(s)	228, 819
FaeI	CATG	1 cut(s)	806
FaqI	GGGAC	2 cut(s)	790, 964
FatI	CATG	1 cut(s)	802
FauI	CCCGC	3 cut(s)	362, 402, 446
FauNDI	CATATG	1 cut(s)	1266
FblI	GTMKAC	1 cut(s)	501
Fnu4HI	GCNGC	5 cut(s)	129, 132, 267, 508, 515
FokI	GGATG	3 cut(s)	92, 679, 1154
FriOI	GRGCYC	1 cut(s)	395
Fsp4HI	GCNGC	5 cut(s)	129, 132, 267, 508, 515
FspBI	CTAG	2 cut(s)	221, 1226
GlaI	GCGC	2 cut(s)	191, 265
GluI	GCNGC	5 cut(s)	129, 132, 267, 508, 515
GsuI	CTGGAG	3 cut(s)	341, 458, 954
HaeIII	GGCC	6 cut(s)	393, 414, 536, 730, 794, 902
HapII	CCGG	3 cut(s)	207, 364, 415
HhaI	GCGC	2 cut(s)	192, 266
Hin1II	CATG	1 cut(s)	806
Hin6I	GCGC	2 cut(s)	190, 264
HinP1I	GCGC	2 cut(s)	190, 264
HincII	GTYRAC	1 cut(s)	340
HindII	GTYRAC	1 cut(s)	340
HinfI	GANTC	2 cut(s)	168, 256
HpaII	CCGG	3 cut(s)	207, 364, 415
HphI	GGTGA	2 cut(s)	49, 337
Hpy166II	GTNNAC	5 cut(s)	260, 340, 502, 676, 829
Hpy188III	TCNNGA	7 cut(s)	174, 445, 541, 775, 860, 971, 1226
Hpy8I	GTNNAC	5 cut(s)	260, 340, 502, 676, 829
HpyAV	CCTTC	7 cut(s)	146, 301, 424, 476, 557, 647, 800
HpyCH4III	ACNGT	1 cut(s)	833
HpyCH4IV	ACGT	1 cut(s)	471
HpyCH4V	TGCA	7 cut(s)	517, 650, 715, 802, 829, 980, 1163
HpyF10VI	GCNNNNNNNGC	6 cut(s)	101, 189, 196, 656, 712, 1198
HpyF3I	CTNAG	1 cut(s)	910
HpySE526I	ACGT	1 cut(s)	471
Hsp92II	CATG	1 cut(s)	806
HspAI	GCGC	2 cut(s)	190, 264
Kzo9I	GATC	9 cut(s)	54, 234, 243, 441, 914, 1119, 1150, 1222, 1307
LmnI	GCTCC	6 cut(s)	209, 319, 366, 433, 703, 973
Lsp1109I	GCAGC	2 cut(s)	140, 501
LweI	GCATC	3 cut(s)	177, 504, 657
MabI	ACCWGGT	1 cut(s)	1095
MaeI	CTAG	2 cut(s)	221, 1226
MaeII	ACGT	1 cut(s)	471
MaeIII	GTNAC	4 cut(s)	325, 380, 848, 931
MalI	GATC	9 cut(s)	56, 236, 245, 443, 916, 1121, 1152, 1224, 1309
MbiI	CCGCTC	1 cut(s)	369
MboI	GATC	9 cut(s)	54, 234, 243, 441, 914, 1119, 1150, 1222, 1307
MboII	GAAGA	3 cut(s)	136, 848, 1065
MfeI	CAATTG	1 cut(s)	1062
MflI	RGATCY	1 cut(s)	1222
MhlI	GDGCHC	3 cut(s)	395, 661, 831
MlsI	TGGCCA	1 cut(s)	902
MluCI	AATT	4 cut(s)	636, 1044, 1062, 1257
MluI	ACGCGT	1 cut(s)	940
MluNI	TGGCCA	1 cut(s)	902
MlyI	GAGTC	2 cut(s)	177, 265
MmeI	TCCRAC	1 cut(s)	354
Mox20I	TGGCCA	1 cut(s)	902
MscI	TGGCCA	1 cut(s)	902
MseI	TTAA	3 cut(s)	582, 996, 1110
MslI	CAYNNNNRTG	2 cut(s)	720, 989
Msp20I	TGGCCA	1 cut(s)	902
MspA1I	CMGCKG	2 cut(s)	319, 507
MspI	CCGG	3 cut(s)	207, 364, 415
MspR9I	CCNGG	2 cut(s)	748, 1097
MunI	CAATTG	1 cut(s)	1062
MvaI	CCWGG	2 cut(s)	748, 1097
MvnI	CGCG	5 cut(s)	192, 264, 446, 453, 942
MwoI	GCNNNNNNNGC	6 cut(s)	101, 189, 196, 656, 712, 1198
NdeI	CATATG	1 cut(s)	1266
NdeII	GATC	9 cut(s)	54, 234, 243, 441, 914, 1119, 1150, 1222, 1307
NlaIII	CATG	1 cut(s)	806
NlaIV	GGNNCC	4 cut(s)	252, 362, 393, 413
NmeAIII	GCCGAG	1 cut(s)	265
NmuCI	GTSAC	3 cut(s)	325, 380, 931
NruI	TCGCGA	1 cut(s)	446
PaeR7I	CTCGAG	1 cut(s)	165
PcsI	WCGNNNNNNNCGW	4 cut(s)	108, 172, 528, 1312
PkrI	GCNGC	5 cut(s)	130, 133, 268, 509, 516
PleI	GAGTC	2 cut(s)	176, 264
PpsI	GAGTC	2 cut(s)	176, 264
Psp6I	CCWGG	2 cut(s)	746, 1095
PspGI	CCWGG	2 cut(s)	746, 1095
PspN4I	GGNNCC	4 cut(s)	252, 362, 393, 413
PspOMI	GGGCCC	1 cut(s)	391
PspPI	GGNCC	4 cut(s)	391, 392, 412, 728
PspXI	VCTCGAGB	1 cut(s)	165
PstI	CTGCAG	1 cut(s)	519
PsuI	RGATCY	1 cut(s)	1222
RruI	TCGCGA	1 cut(s)	446
RsaI	GTAC	3 cut(s)	422, 677, 683
RsaNI	GTAC	3 cut(s)	421, 676, 682
RseI	CAYNNNNRTG	2 cut(s)	720, 989
SaqAI	TTAA	3 cut(s)	582, 996, 1110
SatI	GCNGC	5 cut(s)	129, 132, 267, 508, 515
Sau3AI	GATC	9 cut(s)	54, 234, 243, 441, 914, 1119, 1150, 1222, 1307
Sau96I	GGNCC	4 cut(s)	391, 392, 412, 728
SchI	GAGTC	2 cut(s)	177, 265
ScrFI	CCNGG	2 cut(s)	748, 1097
SduI	GDGCHC	3 cut(s)	395, 661, 831
SexAI	ACCWGGT	1 cut(s)	1095
SfaNI	GCATC	3 cut(s)	177, 504, 657
SfcI	CTRYAG	2 cut(s)	502, 515
Sfr274I	CTCGAG	1 cut(s)	165
SlaI	CTCGAG	1 cut(s)	165
SmiMI	CAYNNNNRTG	2 cut(s)	720, 989
SmlI	CTYRAG	2 cut(s)	165, 1271
SmoI	CTYRAG	2 cut(s)	165, 1271
Sse9I	AATT	4 cut(s)	636, 1044, 1062, 1257
SsiI	CCGC	9 cut(s)	60, 132, 157, 267, 317, 369, 395, 453, 507
SspMI	CTAG	2 cut(s)	221, 1226
StyD4I	CCNGG	2 cut(s)	746, 1095
StyI	CCWWGG	2 cut(s)	228, 819
TaaI	ACNGT	1 cut(s)	833
TaiI	ACGT	1 cut(s)	474
TaqI	TCGA	5 cut(s)	166, 345, 594, 1306, 1315
TaqII	GACCGA	1 cut(s)	1258
TasI	AATT	4 cut(s)	636, 1044, 1062, 1257
TatI	WGTACW	1 cut(s)	681
TauI	GCSGC	3 cut(s)	134, 269, 510
Tru1I	TTAA	3 cut(s)	582, 996, 1110
Tru9I	TTAA	3 cut(s)	582, 996, 1110
TseFI	GTSAC	3 cut(s)	325, 380, 931
TseI	GCWGC	2 cut(s)	128, 514
Tsp45I	GTSAC	3 cut(s)	325, 380, 931
TspDTI	ATGAA	6 cut(s)	312, 776, 819, 1106, 1202, 1253
TspGWI	ACGGA	1 cut(s)	344
VneI	GTGCAC	1 cut(s)	827
XbaI	TCTAGA	1 cut(s)	1225
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1130
XhoI	CTCGAG	1 cut(s)	165
XmiI	GTMKAC	1 cut(s)	501
XspI	CTAG	2 cut(s)	221, 1226

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.