Rroxscaffold_176G00431530

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000176
OG0006729
Physical Location & Seq
Reverse (-)
1234072 .. 1236972
2901 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_176G00431530.1

Sequence Viewer

Length: 708 bp
ATGCAAATGAAAGATCTAGAAGTACAAATCTGGTTATCAAAGAATGACATCCCTAAGAACATGAAGACGCTGATCATGCAAAATGTACACAAACAACTTGAGCAAAACAAAGATGTTCATGTGGGGAATATCCTCTCTATTCTTCCATTGGAGCAGAAAAGATTTATCAAGCGACATCTCTGTTTGCCTATGCTGAAAAAAGTGCCAATGCTTCGAGTTATGGATGAAGAAGTATTGAAGGCAATCTTTGACAATCTTAAGCCAGTGAGAGGCAATTTTCATGGCGAAGAACTACTCAATTGGGCATCTACATTTTCTGCACTGCCCGTCTCCACAAGATTTGTTAAGTCTCTCACAAAAGTAGAAGGGTTTGCTCTAATGGCCAGTGATTTGAAGAGTGTAGCCTCCCAATCGTGGCGGCATTTCAGCGCAGAGATGAACCTAGTTGATTGGTTTAAAGGAATGATTTCAAGTCCCCGTGGGGAGGAGATTTTAGATCCATTGATAGAAGTTCAGCCCTCTCCAAGAGCTTTGAAGCGAGCATTGTTGGTTTGTCTCCGCTGTATAAATGTCCATAAGCGGCCAAAAATGGGGCAAATTGTTCATATGCTTGAAGCAGAAGACTTTCCTTTTCGTTCAGTGGCTGCCGTCTCTAAGCCATTCGGACGACGGATATTCTCCGTCGTTGATAATGCACTTAGACGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans Pkinase_Tyr cNMP_binding

Protein Analysis

235

Amino Acids

27.05

Weight (kDa)

9.85

Isoelectric Point (pI)

52.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 418, 559, 580
AclWI GGATC 1 cut(s) 491
AcoI YGGCCR 2 cut(s) 381, 581
AfaI GTAC 2 cut(s) 24, 87
AfiI CCNNNNNNNGG 4 cut(s) 269, 414, 484, 590
AflII CTTAAG 1 cut(s) 257
AgsI TTSAA 5 cut(s) 238, 394, 471, 535, 614
AluBI AGCT 1 cut(s) 530
AluI AGCT 1 cut(s) 530
Alw26I GTCTC 4 cut(s) 334, 354, 560, 655
AlwI GGATC 1 cut(s) 491
AlwNI CAGNNNCTG 1 cut(s) 644
AoxI GGCC 2 cut(s) 381, 581
ApeKI GCWGC 1 cut(s) 644
Asp700I GAANNNNTTC 2 cut(s) 466, 624
AspLEI GCGC 1 cut(s) 431
BalI TGGCCA 1 cut(s) 383
BbsI GAAGAC 2 cut(s) 71, 627
BbvI GCAGC 1 cut(s) 631
BceAI ACGGC 1 cut(s) 632
BclI TGATCA 1 cut(s) 72
BcoDI GTCTC 4 cut(s) 334, 354, 560, 655
BfaI CTAG 2 cut(s) 17, 443
BfrI CTTAAG 1 cut(s) 257
BglII AGATCT 1 cut(s) 13
BisI GCNGC 3 cut(s) 419, 581, 645
BlsI GCNGC 3 cut(s) 420, 582, 646
BmsI GCATC 1 cut(s) 314
BpiI GAAGAC 2 cut(s) 71, 627
BpuEI CTTGAG 1 cut(s) 119
BsaJI CCNNGG 1 cut(s) 478
Bsc4I CCNNNNNNNGG 4 cut(s) 269, 414, 484, 590
Bse1I ACTGG 2 cut(s) 263, 384
BseDI CCNNGG 1 cut(s) 478
BseGI GGATG 2 cut(s) 48, 229
BseLI CCNNNNNNNGG 4 cut(s) 269, 414, 484, 590
BseNI ACTGG 2 cut(s) 263, 384
BseRI GAGGAG 1 cut(s) 500
BseXI GCAGC 1 cut(s) 631
BsgI GTGCAG 1 cut(s) 303
BshFI GGCC 2 cut(s) 383, 583
BslFI GGGAC 1 cut(s) 459
BslI CCNNNNNNNGG 4 cut(s) 269, 414, 484, 590
BsmAI GTCTC 4 cut(s) 334, 354, 560, 655
BsmBI CGTCTC 2 cut(s) 334, 655
BsmFI GGGAC 1 cut(s) 459
BsnI GGCC 2 cut(s) 383, 583
Bsp1407I TGTACA 1 cut(s) 85
Bsp143I GATC 3 cut(s) 13, 72, 496
BspACI CCGC 3 cut(s) 418, 559, 580
BspANI GGCC 2 cut(s) 383, 583
BspPI GGATC 1 cut(s) 491
BspTI CTTAAG 1 cut(s) 257
BsrGI TGTACA 1 cut(s) 85
BsrI ACTGG 2 cut(s) 263, 384
BssECI CCNNGG 1 cut(s) 478
BssMI GATC 3 cut(s) 13, 72, 496
Bst6I CTCTTC 1 cut(s) 389
BstAFI CTTAAG 1 cut(s) 257
BstAUI TGTACA 1 cut(s) 85
BstC8I GCNNGC 1 cut(s) 540
BstDEI CTNAG 3 cut(s) 54, 654, 698
BstDSI CCRYGG 1 cut(s) 478
BstF5I GGATG 2 cut(s) 48, 229
BstHHI GCGC 1 cut(s) 431
BstKTI GATC 3 cut(s) 16, 75, 499
BstMAI GTCTC 4 cut(s) 334, 354, 560, 655
BstMBI GATC 3 cut(s) 13, 72, 496
BstMWI GCNNNNNNNGC 2 cut(s) 76, 380
BstV1I GCAGC 1 cut(s) 631
BstV2I GAAGAC 2 cut(s) 71, 627
BstX2I RGATCY 2 cut(s) 13, 496
BstYI RGATCY 2 cut(s) 13, 496
BsuRI GGCC 2 cut(s) 383, 583
BtgI CCRYGG 1 cut(s) 478
BtsCI GGATG 2 cut(s) 48, 229
BtsI GCAGTG 1 cut(s) 320
BtsIMutI CAGTG 4 cut(s) 270, 320, 391, 645
Cac8I GCNNGC 1 cut(s) 540
CaiI CAGNNNCTG 1 cut(s) 644
CfoI GCGC 1 cut(s) 431
CseI GACGC 1 cut(s) 76
Csp6I GTAC 2 cut(s) 23, 86
CspCI CAANNNNNGTGG 2 cut(s) 322, 357
CviAII CATG 4 cut(s) 61, 76, 119, 281
CviJI RGCY 8 cut(s) 262, 383, 404, 517, 530, 583, 644, 658
CviKI_1 RGCY 8 cut(s) 262, 383, 404, 517, 530, 583, 644, 658
CviQI GTAC 2 cut(s) 23, 86
DdeI CTNAG 3 cut(s) 54, 654, 698
DpnI GATC 3 cut(s) 15, 74, 498
DpnII GATC 3 cut(s) 13, 72, 496
DraI TTTAAA 1 cut(s) 457
EaeI YGGCCR 2 cut(s) 381, 581
Eam1104I CTCTTC 1 cut(s) 389
EarI CTCTTC 1 cut(s) 389
Esp3I CGTCTC 2 cut(s) 334, 655
FaeI CATG 4 cut(s) 64, 79, 122, 284
FalI AAGNNNNNCTT 2 cut(s) 230, 262
FaqI GGGAC 1 cut(s) 459
FatI CATG 4 cut(s) 60, 75, 118, 280
FauNDI CATATG 1 cut(s) 606
FbaI TGATCA 1 cut(s) 72
Fnu4HI GCNGC 3 cut(s) 419, 581, 645
FokI GGATG 2 cut(s) 35, 236
Fsp4HI GCNGC 3 cut(s) 419, 581, 645
FspBI CTAG 2 cut(s) 17, 443
GlaI GCGC 1 cut(s) 430
GluI GCNGC 3 cut(s) 419, 581, 645
HaeIII GGCC 2 cut(s) 383, 583
HgaI GACGC 1 cut(s) 76
HhaI GCGC 1 cut(s) 431
Hin1II CATG 4 cut(s) 64, 79, 122, 284
Hin6I GCGC 1 cut(s) 429
HinP1I GCGC 1 cut(s) 429
Hpy166II GTNNAC 1 cut(s) 88
Hpy188I TCNGA 1 cut(s) 665
Hpy188III TCNNGA 1 cut(s) 17
Hpy8I GTNNAC 1 cut(s) 88
Hpy99I CGWCG 2 cut(s) 672, 686
HpyAV CCTTC 2 cut(s) 232, 359
HpyCH4V TGCA 4 cut(s) 4, 79, 320, 695
HpyF10VI GCNNNNNNNGC 2 cut(s) 76, 380
HpyF3I CTNAG 3 cut(s) 54, 654, 698
Hsp92II CATG 4 cut(s) 64, 79, 122, 284
HspAI GCGC 1 cut(s) 429
Ksp22I TGATCA 1 cut(s) 72
Kzo9I GATC 3 cut(s) 13, 72, 496
LmnI GCTCC 1 cut(s) 151
LpnPI CCDG 3 cut(s) 16, 276, 397
Lsp1109I GCAGC 1 cut(s) 631
LweI GCATC 1 cut(s) 314
MaeI CTAG 2 cut(s) 17, 443
MalI GATC 3 cut(s) 15, 74, 498
MboI GATC 3 cut(s) 13, 72, 496
MboII GAAGA 6 cut(s) 76, 134, 239, 299, 406, 632
MfeI CAATTG 1 cut(s) 298
MflI RGATCY 2 cut(s) 13, 496
MlsI TGGCCA 1 cut(s) 383
MluCI AATT 3 cut(s) 274, 298, 597
MluNI TGGCCA 1 cut(s) 383
MnlI CCTC 5 cut(s) 143, 263, 415, 478, 529
Mox20I TGGCCA 1 cut(s) 383
MroXI GAANNNNTTC 2 cut(s) 466, 624
MscI TGGCCA 1 cut(s) 383
MseI TTAA 3 cut(s) 258, 345, 456
Msp20I TGGCCA 1 cut(s) 383
MspA1I CMGCKG 1 cut(s) 561
MspCI CTTAAG 1 cut(s) 257
MunI CAATTG 1 cut(s) 298
MwoI GCNNNNNNNGC 2 cut(s) 76, 380
NdeI CATATG 1 cut(s) 606
NdeII GATC 3 cut(s) 13, 72, 496
NlaIII CATG 4 cut(s) 64, 79, 122, 284
PdmI GAANNNNTTC 2 cut(s) 466, 624
PkrI GCNGC 3 cut(s) 420, 582, 646
PstNI CAGNNNCTG 1 cut(s) 644
PsuI RGATCY 2 cut(s) 13, 496
RsaI GTAC 2 cut(s) 24, 87
RsaNI GTAC 2 cut(s) 23, 86
SaqAI TTAA 3 cut(s) 258, 345, 456
SatI GCNGC 3 cut(s) 419, 581, 645
Sau3AI GATC 3 cut(s) 13, 72, 496
SetI ASST 2 cut(s) 444, 532
SfaNI GCATC 1 cut(s) 314
SmlI CTYRAG 2 cut(s) 98, 257
SmoI CTYRAG 2 cut(s) 98, 257
Sse9I AATT 3 cut(s) 274, 298, 597
SsiI CCGC 3 cut(s) 418, 559, 580
SspMI CTAG 2 cut(s) 17, 443
TaqI TCGA 1 cut(s) 214
TasI AATT 3 cut(s) 274, 298, 597
TatI WGTACW 2 cut(s) 22, 85
TauI GCSGC 2 cut(s) 421, 583
Tru1I TTAA 3 cut(s) 258, 345, 456
Tru9I TTAA 3 cut(s) 258, 345, 456
TscAI CASTG 4 cut(s) 270, 327, 391, 645
TseI GCWGC 1 cut(s) 644
TspDTI ATGAA 7 cut(s) 23, 77, 107, 240, 269, 452, 593
TspGWI ACGGA 2 cut(s) 670, 685
TspRI CASTG 4 cut(s) 270, 327, 391, 645
Vha464I CTTAAG 1 cut(s) 257
XbaI TCTAGA 1 cut(s) 16
XmnI GAANNNNTTC 2 cut(s) 466, 624
XspI CTAG 2 cut(s) 17, 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.