MD10G1080400.v1.1

pfkB family carbohydrate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009119
Physical Location & Seq
Forward (+)
11864340 .. 11868479
4140 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1080400.v1.1.491

Sequence Viewer

Length: 1290 bp
ATGGAGGAAAGCAGTGCACAGAGGCGACTGGATTCAATTTCTCGTCACCTGCTATTGCAAACTCCTCAACCTAACCATGGCCTTCAACAGGCGGTGTTATTAAGTAATGGGAAATCGGAAAGTGAAGGGTCAGAGGCTGTGGTGATAGGAGGCATGGTGTTGGACATTCATGCCACACCTTCCATCCCTTCAAATCCCAGAACCACCACTCCTGGCAAGGTCAGCTATGTATTAGGAGGGGTAGCAAGGAATGTTGCTGAGTGCATGTCAAAGCTAGGAGCTAAGCCCTTCATGATTAGCGCCTTGGGCCTTGACATGGCAGGAAACTTATTGTTGGAGCACTGGAAGTCTTCTGGGCTATCTATGGAAGGCATTTTGAAGCACCAAAATGTTGAGACTCCTGTTGTGTGCAACGTACTTGATGTTAATGGGGAGGTGGCCGCTGGTGTTGCGAGTGTAGAAGCTCTCGAAAAGTTTCTGACGTCTGAGTGGATCCAACAGTTTAAATACAGTATATGCTCTGCTCCAGTGTTGATGATTGATGCAAACCTGAATCTTTCTGCTCTAAAAGCTTCTTGCCAATTGGCAGCAGAATCTAAAATTCCAGTGTGGTTTGAGCCTGTATCGGTAGCAAAATCTAGAAGAATCAATTCAATTTCCAAGTATGTAAGTTTTGCTTCACCCAACGAGGATGAACTTGTTGCCATGGCAAATGCTTTATCCGGTGGAAATGTGTTTTCTCCAATTGAAAGGGACAGTAGCAGAAATCAATGCTCAACCGAAACCTTGTTCCATATGCTAAAACCCGCGATCTGGGTTTTGCTAGAGAAAGGTATCAAAATAGTTGTTGTGACCGTTGGTTCGAATGGAGTGTTCTTATGCTCTAAAGGAGGGCCAAGCTTCATGAGAACTGTTTGCAAGGGAAACAAACCATATGTTTCCAATGCGGAATTGTTTAACACTGTGACTGCAAGTTGTCCATCAAATACGTTCTCAAATCCTTCAGATTCTGACAAAAGCTCGTTTCTCTTTGCTGTGCATTTTCCTGCACTTCCTGCATCGGTGGTAAGGCTTACAGGAGCTGGCGATTGCTTGGTTGGGGGGACTGTTGCTTCCATTTGTGCAGGTTTAGATGTCATGCAAAGTCTAGCAGTTGGTATTGCAGCTTCCAAAGCGGCAGTTGAGGCAGAGGCCAATGTGCCTCGTGTACTTAATCTTGTCGCAATAGCAGATGATGCAAAGTTGGTATACTCGGCTGCAAAAGTTGTGTTCCATCAATCAGTGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MaoC_dehydratas PfkB

Protein Analysis

430

Amino Acids

45.31

Weight (kDa)

6.46

Isoelectric Point (pI)

36.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 46 - 305 5.2e-25 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 57
AatII GACGTC 1 cut(s) 485
Acc36I ACCTGC 2 cut(s) 57, 1115
AccI GTMKAC 1 cut(s) 1248
AccII CGCG 1 cut(s) 809
AciI CCGC 5 cut(s) 92, 441, 807, 947, 1175
AclWI GGATC 2 cut(s) 487, 500
AcoI YGGCCR 1 cut(s) 438
AcsI RAATTY 1 cut(s) 600
AcuI CTGAAG 1 cut(s) 987
AcyI GRCGYC 1 cut(s) 482
AfaI GTAC 2 cut(s) 417, 1209
AfiI CCNNNNNNNGG 4 cut(s) 77, 88, 316, 813
AgsI TTSAA 6 cut(s) 36, 86, 192, 379, 654, 749
AjnI CCWGG 1 cut(s) 211
AloI GAACNNNNNNTCC 2 cut(s) 193, 225
AluBI AGCT 9 cut(s) 225, 274, 281, 464, 572, 900, 1020, 1082, 1166
AluI AGCT 9 cut(s) 225, 274, 281, 464, 572, 900, 1020, 1082, 1166
Alw21I GWGCWC 2 cut(s) 19, 342
Alw26I GTCTC 1 cut(s) 389
Alw44I GTGCAC 1 cut(s) 15
AlwI GGATC 2 cut(s) 487, 500
AlwNI CAGNNNCTG 3 cut(s) 137, 1010, 1082
AoxI GGCC 5 cut(s) 79, 307, 438, 893, 1191
ApaLI GTGCAC 1 cut(s) 15
ApeKI GCWGC 3 cut(s) 587, 1163, 1256
ApoI RAATTY 1 cut(s) 600
ArsI GACNNNNNNTTYG 2 cut(s) 844, 876
Asp700I GAANNNNTTC 2 cut(s) 474, 649
AspLEI GCGC 1 cut(s) 302
AspS9I GGNCC 2 cut(s) 307, 893
AsuHPI GGTGA 3 cut(s) 38, 154, 672
AsuII TTCGAA 1 cut(s) 863
BaeGI GKGCMC 1 cut(s) 19
BamHI GGATCC 1 cut(s) 492
BarI GAAGNNNNNNTAC 2 cut(s) 1150, 1182
BauI CACGAG 1 cut(s) 1203
BbsI GAAGAC 1 cut(s) 342
Bbv12I GWGCWC 2 cut(s) 19, 342
BbvI GCAGC 3 cut(s) 599, 1175, 1243
BccI CCATC 3 cut(s) 191, 988, 1281
BciT130I CCWGG 1 cut(s) 213
BcoDI GTCTC 1 cut(s) 389
BfaI CTAG 4 cut(s) 275, 639, 824, 1148
BfoI RGCGCY 1 cut(s) 303
BfuAI ACCTGC 2 cut(s) 57, 1115
BisI GCNGC 5 cut(s) 441, 588, 1164, 1176, 1257
BlpI GCTNAGC 1 cut(s) 282
BlsI GCNGC 5 cut(s) 442, 589, 1165, 1177, 1258
Bme1390I CCNGG 1 cut(s) 213
BmgT120I GGNCC 2 cut(s) 307, 893
BmiI GGNNCC 1 cut(s) 494
BmrFI CCNGG 1 cut(s) 213
BmsI GCATC 3 cut(s) 532, 1067, 1225
BpiI GAAGAC 1 cut(s) 342
BpmI CTGGAG 1 cut(s) 510
Bpu1102I GCTNAGC 1 cut(s) 282
Bpu14I TTCGAA 1 cut(s) 863
BsaHI GRCGYC 1 cut(s) 482
BsaJI CCNNGG 3 cut(s) 76, 303, 705
BsaWI WCCGGW 1 cut(s) 722
BsaXI ACNNNNNCTCC 4 cut(s) 141, 171, 193, 223
Bsc4I CCNNNNNNNGG 4 cut(s) 77, 88, 316, 813
Bse1I ACTGG 4 cut(s) 33, 347, 527, 605
BseBI CCWGG 1 cut(s) 213
BseDI CCNNGG 3 cut(s) 76, 303, 705
BseGI GGATG 2 cut(s) 183, 697
BseLI CCNNNNNNNGG 4 cut(s) 77, 88, 316, 813
BseMII CTCAG 2 cut(s) 249, 477
BseNI ACTGG 4 cut(s) 33, 347, 527, 605
BseRI GAGGAG 1 cut(s) 54
BseSI GKGCMC 1 cut(s) 19
BseXI GCAGC 3 cut(s) 599, 1175, 1243
BsgI GTGCAG 2 cut(s) 1032, 1143
Bsh1236I CGCG 1 cut(s) 809
BshFI GGCC 5 cut(s) 81, 309, 440, 895, 1193
BsiHKAI GWGCWC 2 cut(s) 19, 342
BsiSI CCGG 1 cut(s) 723
BslFI GGGAC 2 cut(s) 767, 1117
BslI CCNNNNNNNGG 4 cut(s) 77, 88, 316, 813
BsmAI GTCTC 1 cut(s) 389
BsmFI GGGAC 2 cut(s) 767, 1117
BsnI GGCC 5 cut(s) 81, 309, 440, 895, 1193
Bsp119I TTCGAA 1 cut(s) 863
Bsp1286I GDGCHC 2 cut(s) 19, 342
Bsp143I GATC 2 cut(s) 492, 810
Bsp1720I GCTNAGC 1 cut(s) 282
Bsp19I CCATGG 2 cut(s) 76, 705
BspACI CCGC 5 cut(s) 92, 441, 807, 947, 1175
BspANI GGCC 5 cut(s) 81, 309, 440, 895, 1193
BspCNI CTCAG 2 cut(s) 250, 478
BspFNI CGCG 1 cut(s) 809
BspHI TCATGA 2 cut(s) 291, 903
BspLI GGNNCC 1 cut(s) 494
BspMI ACCTGC 2 cut(s) 57, 1115
BspPI GGATC 2 cut(s) 487, 500
BspT104I TTCGAA 1 cut(s) 863
BsrI ACTGG 4 cut(s) 33, 347, 527, 605
BssECI CCNNGG 3 cut(s) 76, 303, 705
BssMI GATC 2 cut(s) 492, 810
BssNAI GTATAC 1 cut(s) 1249
BssNI GRCGYC 1 cut(s) 482
BssSI CACGAG 1 cut(s) 1203
BssT1I CCWWGG 3 cut(s) 76, 303, 705
Bst1107I GTATAC 1 cut(s) 1249
Bst2BI CACGAG 1 cut(s) 1203
Bst2UI CCWGG 1 cut(s) 213
Bst4CI ACNGT 7 cut(s) 501, 512, 758, 856, 913, 964, 1108
BstACI GRCGYC 1 cut(s) 482
BstAPI GCANNNNNTGC 2 cut(s) 1055, 1235
BstBI TTCGAA 1 cut(s) 863
BstC8I GCNNGC 1 cut(s) 1084
BstDEI CTNAG 3 cut(s) 258, 282, 486
BstDSI CCRYGG 2 cut(s) 76, 705
BstENI CCTNNNNNAGG 1 cut(s) 86
BstF5I GGATG 2 cut(s) 183, 697
BstFNI CGCG 1 cut(s) 809
BstH2I RGCGCY 1 cut(s) 303
BstHHI GCGC 1 cut(s) 302
BstKTI GATC 2 cut(s) 495, 813
BstMAI GTCTC 1 cut(s) 389
BstMBI GATC 2 cut(s) 492, 810
BstMWI GCNNNNNNNGC 8 cut(s) 222, 306, 449, 569, 1055, 1172, 1184, 1235
BstNI CCWGG 1 cut(s) 213
BstNSI RCATGY 1 cut(s) 268
BstSCI CCNGG 1 cut(s) 211
BstSLI GKGCMC 1 cut(s) 19
BstUI CGCG 1 cut(s) 809
BstV1I GCAGC 3 cut(s) 599, 1175, 1243
BstV2I GAAGAC 1 cut(s) 342
BstX2I RGATCY 1 cut(s) 492
BstYI RGATCY 1 cut(s) 492
BstZ17I GTATAC 1 cut(s) 1249
BsuRI GGCC 5 cut(s) 81, 309, 440, 895, 1193
BtgI CCRYGG 2 cut(s) 76, 705
BtsCI GGATG 2 cut(s) 183, 697
BtsI GCAGTG 1 cut(s) 19
BtsIMutI CAGTG 6 cut(s) 19, 340, 534, 612, 960, 1287
BveI ACCTGC 2 cut(s) 57, 1115
Cac8I GCNNGC 1 cut(s) 1084
CaiI CAGNNNCTG 3 cut(s) 137, 1010, 1082
CciI TCATGA 2 cut(s) 291, 903
CfoI GCGC 1 cut(s) 302
Cfr13I GGNCC 2 cut(s) 307, 893
Csp6I GTAC 2 cut(s) 416, 1208
CviAII CATG 9 cut(s) 77, 154, 170, 265, 292, 316, 706, 904, 1138
CviQI GTAC 2 cut(s) 416, 1208
DdeI CTNAG 3 cut(s) 258, 282, 486
DpnI GATC 2 cut(s) 494, 812
DpnII GATC 2 cut(s) 492, 810
DraI TTTAAA 1 cut(s) 505
EaeI YGGCCR 1 cut(s) 438
Eco130I CCWWGG 3 cut(s) 76, 303, 705
Eco57I CTGAAG 1 cut(s) 987
EcoNI CCTNNNNNAGG 1 cut(s) 86
EcoRII CCWGG 1 cut(s) 211
EcoT14I CCWWGG 3 cut(s) 76, 303, 705
ErhI CCWWGG 3 cut(s) 76, 303, 705
FaeI CATG 9 cut(s) 80, 157, 173, 268, 295, 319, 709, 907, 1141
FalI AAGNNNNNCTT 2 cut(s) 661, 693
FaqI GGGAC 2 cut(s) 767, 1117
FatI CATG 9 cut(s) 76, 153, 169, 264, 291, 315, 705, 903, 1137
FauI CCCGC 1 cut(s) 814
FauNDI CATATG 2 cut(s) 795, 934
FblI GTMKAC 1 cut(s) 1248
Fnu4HI GCNGC 5 cut(s) 441, 588, 1164, 1176, 1257
FokI GGATG 2 cut(s) 170, 704
Fsp4HI GCNGC 5 cut(s) 441, 588, 1164, 1176, 1257
FspBI CTAG 4 cut(s) 275, 639, 824, 1148
GlaI GCGC 1 cut(s) 301
GluI GCNGC 5 cut(s) 441, 588, 1164, 1176, 1257
GsuI CTGGAG 1 cut(s) 510
HaeII RGCGCY 1 cut(s) 303
HaeIII GGCC 5 cut(s) 81, 309, 440, 895, 1193
HapII CCGG 1 cut(s) 723
HhaI GCGC 1 cut(s) 302
Hin1I GRCGYC 1 cut(s) 482
Hin1II CATG 9 cut(s) 80, 157, 173, 268, 295, 319, 709, 907, 1141
Hin6I GCGC 1 cut(s) 300
HinP1I GCGC 1 cut(s) 300
HindIII AAGCTT 2 cut(s) 570, 898
HinfI GANTC 6 cut(s) 32, 397, 553, 593, 645, 1007
HpaII CCGG 1 cut(s) 723
HphI GGTGA 3 cut(s) 38, 154, 672
Hpy166II GTNNAC 3 cut(s) 17, 1208, 1249
Hpy188I TCNGA 6 cut(s) 118, 133, 480, 487, 1006, 1012
Hpy188III TCNNGA 4 cut(s) 292, 467, 639, 904
Hpy8I GTNNAC 3 cut(s) 17, 1208, 1249
HpyAV CCTTC 7 cut(s) 92, 119, 189, 198, 298, 362, 1011
HpyCH4III ACNGT 7 cut(s) 501, 512, 758, 856, 913, 964, 1108
HpyCH4IV ACGT 3 cut(s) 414, 482, 989
HpyF10VI GCNNNNNNNGC 8 cut(s) 222, 306, 449, 569, 1055, 1172, 1184, 1235
HpyF3I CTNAG 3 cut(s) 258, 282, 486
HpySE526I ACGT 3 cut(s) 414, 482, 989
Hsp92I GRCGYC 1 cut(s) 482
Hsp92II CATG 9 cut(s) 80, 157, 173, 268, 295, 319, 709, 907, 1141
HspAI GCGC 1 cut(s) 300
Kzo9I GATC 2 cut(s) 492, 810
LmnI GCTCC 4 cut(s) 278, 337, 529, 1079
Lsp1109I GCAGC 3 cut(s) 599, 1175, 1243
LweI GCATC 3 cut(s) 532, 1067, 1225
MaeI CTAG 4 cut(s) 275, 639, 824, 1148
MaeII ACGT 3 cut(s) 414, 482, 989
MaeIII GTNAC 3 cut(s) 44, 850, 964
MalI GATC 2 cut(s) 494, 812
MboI GATC 2 cut(s) 492, 810
MboII GAAGA 2 cut(s) 342, 654
MfeI CAATTG 2 cut(s) 581, 744
MflI RGATCY 1 cut(s) 492
MhlI GDGCHC 2 cut(s) 19, 342
MluCI AATT 7 cut(s) 36, 581, 600, 649, 654, 744, 950
MlyI GAGTC 1 cut(s) 391
MmeI TCCRAC 3 cut(s) 141, 315, 520
MroXI GAANNNNTTC 2 cut(s) 474, 649
MseI TTAA 6 cut(s) 101, 426, 504, 957, 1212, 1288
MslI CAYNNNNRTG 1 cut(s) 387
MspA1I CMGCKG 1 cut(s) 443
MspI CCGG 1 cut(s) 723
MspR9I CCNGG 1 cut(s) 213
MunI CAATTG 2 cut(s) 581, 744
MvaI CCWGG 1 cut(s) 213
MvnI CGCG 1 cut(s) 809
MwoI GCNNNNNNNGC 8 cut(s) 222, 306, 449, 569, 1055, 1172, 1184, 1235
NcoI CCATGG 2 cut(s) 76, 705
NdeI CATATG 2 cut(s) 795, 934
NdeII GATC 2 cut(s) 492, 810
NlaIII CATG 9 cut(s) 80, 157, 173, 268, 295, 319, 709, 907, 1141
NlaIV GGNNCC 1 cut(s) 494
NmeAIII GCCGAG 1 cut(s) 1232
NmuCI GTSAC 3 cut(s) 44, 850, 964
NspI RCATGY 1 cut(s) 268
NspV TTCGAA 1 cut(s) 863
PagI TCATGA 2 cut(s) 291, 903
PaqCI CACCTGC 1 cut(s) 57
PdmI GAANNNNTTC 2 cut(s) 474, 649
PfeI GAWTC 5 cut(s) 32, 553, 593, 645, 1007
PkrI GCNGC 5 cut(s) 442, 589, 1165, 1177, 1258
PleI GAGTC 1 cut(s) 391
PpsI GAGTC 1 cut(s) 391
Psp6I CCWGG 1 cut(s) 211
PspGI CCWGG 1 cut(s) 211
PspN4I GGNNCC 1 cut(s) 494
PspPI GGNCC 2 cut(s) 307, 893
PstNI CAGNNNCTG 3 cut(s) 137, 1010, 1082
PsuI RGATCY 1 cut(s) 492
RsaI GTAC 2 cut(s) 417, 1209
RsaNI GTAC 2 cut(s) 416, 1208
RseI CAYNNNNRTG 1 cut(s) 387
SaqAI TTAA 6 cut(s) 101, 426, 504, 957, 1212, 1288
SatI GCNGC 5 cut(s) 441, 588, 1164, 1176, 1257
Sau3AI GATC 2 cut(s) 492, 810
Sau96I GGNCC 2 cut(s) 307, 893
SchI GAGTC 1 cut(s) 391
ScrFI CCNGG 1 cut(s) 213
SduI GDGCHC 2 cut(s) 19, 342
SfaNI GCATC 3 cut(s) 532, 1067, 1225
SfuI TTCGAA 1 cut(s) 863
SmiMI CAYNNNNRTG 1 cut(s) 387
Sse9I AATT 7 cut(s) 36, 581, 600, 649, 654, 744, 950
SsiI CCGC 5 cut(s) 92, 441, 807, 947, 1175
SspMI CTAG 4 cut(s) 275, 639, 824, 1148
StyD4I CCNGG 1 cut(s) 211
StyI CCWWGG 3 cut(s) 76, 303, 705
TaaI ACNGT 7 cut(s) 501, 512, 758, 856, 913, 964, 1108
TaiI ACGT 3 cut(s) 417, 485, 992
TaqI TCGA 2 cut(s) 468, 863
TasI AATT 7 cut(s) 36, 581, 600, 649, 654, 744, 950
TatI WGTACW 1 cut(s) 1207
TauI GCSGC 2 cut(s) 443, 1178
TfiI GAWTC 5 cut(s) 32, 553, 593, 645, 1007
Tru1I TTAA 6 cut(s) 101, 426, 504, 957, 1212, 1288
Tru9I TTAA 6 cut(s) 101, 426, 504, 957, 1212, 1288
TscAI CASTG 6 cut(s) 19, 347, 534, 612, 967, 1287
TseFI GTSAC 3 cut(s) 44, 850, 964
TseI GCWGC 3 cut(s) 587, 1163, 1256
Tsp45I GTSAC 3 cut(s) 44, 850, 964
TspDTI ATGAA 4 cut(s) 158, 280, 708, 892
TspRI CASTG 6 cut(s) 19, 347, 534, 612, 967, 1287
VneI GTGCAC 1 cut(s) 15
XagI CCTNNNNNAGG 1 cut(s) 86
XapI RAATTY 1 cut(s) 600
XbaI TCTAGA 1 cut(s) 638
XceI RCATGY 1 cut(s) 268
XmiI GTMKAC 1 cut(s) 1248
XmnI GAANNNNTTC 2 cut(s) 474, 649
XspI CTAG 4 cut(s) 275, 639, 824, 1148
ZraI GACGTC 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.