MD10G1086600.v1.1

Diphthamide biosynthesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002470
Physical Location & Seq
Forward (+)
12814070 .. 12816181
2112 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1086600.v1.1.491

Sequence Viewer

Length: 249 bp
ATGTCTTACGACGACGTAGAGATCGAAGACATGGAGTGGAACGAGGAGCTGCAGGCGTACACGTACCCGTGCCCGTGCGGGGACTTATTTCAGATCACAAAGGACGACCTGAAATTGGGGGAAGAGATCGCTCGGTGCCCTAGCTGCTCTCTCTACATCACCGTCATTTACAACATCGAAGATTTCTTGGATAAGAAGAGCAACAAGCCCCTCGAGCCCTCCAAGCAGCAACCAATCACTGTCGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CSL

Protein Analysis

83

Amino Acids

9.48

Weight (kDa)

4.12

Isoelectric Point (pI)

74.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_CSL PF05207 4 - 58 1.4e-24 CSL zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 135
AciI CCGC 1 cut(s) 78
AfaI GTAC 2 cut(s) 59, 65
AfiI CCNNNNNNNGG 2 cut(s) 79, 115
AflIII ACRYGT 1 cut(s) 60
AluBI AGCT 2 cut(s) 49, 144
AluI AGCT 2 cut(s) 49, 144
Ama87I CYCGRG 1 cut(s) 212
ApeKI GCWGC 3 cut(s) 49, 144, 226
AsuHPI GGTGA 1 cut(s) 151
AvaI CYCGRG 1 cut(s) 212
BaeGI GKGCMC 2 cut(s) 74, 140
BanI GGYRCC 1 cut(s) 135
BanII GRGCYC 1 cut(s) 219
BbsI GAAGAC 1 cut(s) 33
BbvI GCAGC 3 cut(s) 36, 131, 238
BfaI CTAG 1 cut(s) 141
BfmI CTRYAG 1 cut(s) 50
BisI GCNGC 3 cut(s) 50, 145, 227
BlsI GCNGC 3 cut(s) 51, 146, 228
BmeT110I CYCGRG 1 cut(s) 212
BmiI GGNNCC 1 cut(s) 137
BpiI GAAGAC 1 cut(s) 33
BsaAI YACGTR 1 cut(s) 63
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 115
BseLI CCNNNNNNNGG 2 cut(s) 79, 115
BseRI GAGGAG 1 cut(s) 59
BseSI GKGCMC 2 cut(s) 74, 140
BseXI GCAGC 3 cut(s) 36, 131, 238
BshNI GGYRCC 1 cut(s) 135
BsiHKCI CYCGRG 1 cut(s) 212
BslFI GGGAC 1 cut(s) 95
BslI CCNNNNNNNGG 2 cut(s) 79, 115
BsmFI GGGAC 1 cut(s) 95
BsoBI CYCGRG 1 cut(s) 212
Bsp1286I GDGCHC 3 cut(s) 74, 140, 219
Bsp143I GATC 3 cut(s) 21, 93, 126
BspACI CCGC 1 cut(s) 78
BspLI GGNNCC 1 cut(s) 137
BspMAI CTGCAG 1 cut(s) 54
BspQI GCTCTTC 1 cut(s) 191
BspT107I GGYRCC 1 cut(s) 135
BssMI GATC 3 cut(s) 21, 93, 126
Bst4CI ACNGT 2 cut(s) 163, 241
Bst6I CTCTTC 2 cut(s) 117, 191
BstBAI YACGTR 1 cut(s) 63
BstC8I GCNNGC 1 cut(s) 54
BstKTI GATC 3 cut(s) 24, 96, 129
BstMBI GATC 3 cut(s) 21, 93, 126
BstMWI GCNNNNNNNGC 3 cut(s) 144, 214, 223
BstSFI CTRYAG 1 cut(s) 50
BstSLI GKGCMC 2 cut(s) 74, 140
BstV1I GCAGC 3 cut(s) 36, 131, 238
BstV2I GAAGAC 1 cut(s) 33
BtsIMutI CAGTG 1 cut(s) 237
Cac8I GCNNGC 1 cut(s) 54
Csp6I GTAC 2 cut(s) 58, 64
CviAII CATG 2 cut(s) 31, 246
CviJI RGCY 4 cut(s) 49, 144, 208, 217
CviKI_1 RGCY 4 cut(s) 49, 144, 208, 217
CviQI GTAC 2 cut(s) 58, 64
DpnI GATC 3 cut(s) 23, 95, 128
DpnII GATC 3 cut(s) 21, 93, 126
Eam1104I CTCTTC 2 cut(s) 117, 191
EarI CTCTTC 2 cut(s) 117, 191
Eco24I GRGCYC 1 cut(s) 219
Eco88I CYCGRG 1 cut(s) 212
EcoT38I GRGCYC 1 cut(s) 219
FaeI CATG 2 cut(s) 34, 249
FaiI YATR 2 cut(s) 32, 247
FaqI GGGAC 1 cut(s) 95
FatI CATG 2 cut(s) 30, 245
FauI CCCGC 1 cut(s) 71
Fnu4HI GCNGC 3 cut(s) 50, 145, 227
FriOI GRGCYC 1 cut(s) 219
Fsp4HI GCNGC 3 cut(s) 50, 145, 227
FspBI CTAG 1 cut(s) 141
GluI GCNGC 3 cut(s) 50, 145, 227
Hin1II CATG 2 cut(s) 34, 249
HphI GGTGA 1 cut(s) 151
Hpy166II GTNNAC 1 cut(s) 60
Hpy188I TCNGA 1 cut(s) 93
Hpy8I GTNNAC 1 cut(s) 60
Hpy99I CGWCG 2 cut(s) 14, 17
HpyCH4III ACNGT 2 cut(s) 163, 241
HpyCH4IV ACGT 2 cut(s) 15, 62
HpyCH4V TGCA 1 cut(s) 52
HpyF10VI GCNNNNNNNGC 3 cut(s) 144, 214, 223
HpySE526I ACGT 2 cut(s) 15, 62
Hsp92II CATG 2 cut(s) 34, 249
Kzo9I GATC 3 cut(s) 21, 93, 126
LguI GCTCTTC 1 cut(s) 191
LmnI GCTCC 1 cut(s) 46
LpnPI CCDG 2 cut(s) 38, 122
Lsp1109I GCAGC 3 cut(s) 36, 131, 238
MaeI CTAG 1 cut(s) 141
MaeII ACGT 2 cut(s) 15, 62
MalI GATC 3 cut(s) 23, 95, 128
MboI GATC 3 cut(s) 21, 93, 126
MboII GAAGA 4 cut(s) 38, 134, 191, 208
MhlI GDGCHC 3 cut(s) 74, 140, 219
MluCI AATT 1 cut(s) 113
MnlI CCTC 3 cut(s) 37, 221, 229
MwoI GCNNNNNNNGC 3 cut(s) 144, 214, 223
NdeII GATC 3 cut(s) 21, 93, 126
NlaIII CATG 2 cut(s) 34, 249
NlaIV GGNNCC 1 cut(s) 137
PaeR7I CTCGAG 1 cut(s) 212
PciSI GCTCTTC 1 cut(s) 191
PcsI WCGNNNNNNNCGW 1 cut(s) 21
PkrI GCNGC 3 cut(s) 51, 146, 228
Ppu21I YACGTR 1 cut(s) 63
PspN4I GGNNCC 1 cut(s) 137
PspXI VCTCGAGB 1 cut(s) 212
PstI CTGCAG 1 cut(s) 54
RsaI GTAC 2 cut(s) 59, 65
RsaNI GTAC 2 cut(s) 58, 64
SapI GCTCTTC 1 cut(s) 191
SatI GCNGC 3 cut(s) 50, 145, 227
Sau3AI GATC 3 cut(s) 21, 93, 126
SduI GDGCHC 3 cut(s) 74, 140, 219
SetI ASST 5 cut(s) 18, 51, 65, 111, 146
SfcI CTRYAG 1 cut(s) 50
Sfr274I CTCGAG 1 cut(s) 212
SlaI CTCGAG 1 cut(s) 212
SmlI CTYRAG 1 cut(s) 212
SmoI CTYRAG 1 cut(s) 212
Sse9I AATT 1 cut(s) 113
SsiI CCGC 1 cut(s) 78
SspMI CTAG 1 cut(s) 141
TaaI ACNGT 2 cut(s) 163, 241
TaiI ACGT 2 cut(s) 18, 65
TaqI TCGA 3 cut(s) 24, 177, 213
TasI AATT 1 cut(s) 113
TscAI CASTG 1 cut(s) 244
TseI GCWGC 3 cut(s) 49, 144, 226
TspRI CASTG 1 cut(s) 244
XhoI CTCGAG 1 cut(s) 212
XspI CTAG 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.