RLG00000014486

Diphthamide biosynthesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0002470
Physical Location & Seq
Forward (+)
53848004 .. 53851242
3239 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014486

Sequence Viewer

Length: 522 bp
ATGTCGTACGACGACGTGGAGATCGAAGATATGGAGTGGAACGACGAGCTCCAGGCGTACACGTACCCGTGCCCATGCGGAGACTTGTTCCAGATCACCAAGGAAGACCTGAAATTGGGGGAAGAGATCGCTCGGTGCCCTAGTTCAACAATCCCTCACCGTCGCTTAGGATTTCAGATGGCCTTGTTATGGAGGCAGCCTAGTGGTGGAAGGATTCTTCTGAAGTTTTGTGATTTTAACTCAGTAGTGGGTGTGCAAGGCTGCATCACCAATTTCACAAGCCCTAAAACTCCATCTCCTACCCTAACAATGGTGTTCGAGGCGAGGTTCTCCTCCTTCAAGAAATCAGACTCCTCCAACAGTCCTGATGGCTCCACCAACAGCCCCGATCACACTGCTTCAACTCCCCAAGCAGATTTGATAAGAAGAAATCCAAATCCACCATCAAAGACGATTCCAAAGCCGCGAGTACAAGCTTCGATGGCGGCGGCGGAGGGGTTTTGGCTCGGCGTTGGGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CSL

Protein Analysis

174

Amino Acids

19.12

Weight (kDa)

5.39

Isoelectric Point (pI)

53.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_CSL PF05207 4 - 48 1.7e-16 CSL zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 135
AccII CGCG 1 cut(s) 466
AciI CCGC 5 cut(s) 78, 464, 485, 488, 491
AcuI CTGAAG 1 cut(s) 242
AfaI GTAC 4 cut(s) 8, 59, 65, 471
AfiI CCNNNNNNNGG 4 cut(s) 115, 189, 206, 310
AflIII ACRYGT 1 cut(s) 60
AgsI TTSAA 3 cut(s) 147, 340, 402
AjiI CACGTC 1 cut(s) 16
AjnI CCWGG 1 cut(s) 51
AluBI AGCT 2 cut(s) 49, 476
AluI AGCT 2 cut(s) 49, 476
Alw21I GWGCWC 1 cut(s) 51
Alw26I GTCTC 1 cut(s) 75
AoxI GGCC 1 cut(s) 180
ApeKI GCWGC 2 cut(s) 196, 261
AsuHPI GGTGA 3 cut(s) 88, 149, 259
BaeGI GKGCMC 2 cut(s) 74, 140
BanI GGYRCC 1 cut(s) 135
BanII GRGCYC 1 cut(s) 51
BbsI GAAGAC 1 cut(s) 111
Bbv12I GWGCWC 1 cut(s) 51
BbvI GCAGC 2 cut(s) 208, 248
BccI CCATC 5 cut(s) 172, 301, 362, 451, 475
BcgI CGANNNNNNTGC 2 cut(s) 377, 411
BciT130I CCWGG 1 cut(s) 53
BcoDI GTCTC 1 cut(s) 75
BfaI CTAG 3 cut(s) 141, 201, 520
BisI GCNGC 5 cut(s) 197, 262, 464, 486, 489
BlsI GCNGC 5 cut(s) 198, 263, 465, 487, 490
Bme1390I CCNGG 1 cut(s) 53
BmgBI CACGTC 1 cut(s) 16
BmiI GGNNCC 2 cut(s) 137, 373
BmrFI CCNGG 1 cut(s) 53
BmsI GCATC 1 cut(s) 273
BpiI GAAGAC 1 cut(s) 111
BpmI CTGGAG 1 cut(s) 35
Bpu10I CCTNAGC 1 cut(s) 166
BsaAI YACGTR 1 cut(s) 63
BsaJI CCNNGG 1 cut(s) 99
BsaXI ACNNNNNCTCC 2 cut(s) 280, 310
Bsc4I CCNNNNNNNGG 4 cut(s) 115, 189, 206, 310
BseBI CCWGG 1 cut(s) 53
BseDI CCNNGG 1 cut(s) 99
BseLI CCNNNNNNNGG 4 cut(s) 115, 189, 206, 310
BseMII CTCAG 1 cut(s) 255
BseRI GAGGAG 2 cut(s) 322, 343
BseSI GKGCMC 2 cut(s) 74, 140
BseXI GCAGC 2 cut(s) 208, 248
Bsh1236I CGCG 1 cut(s) 466
BshFI GGCC 1 cut(s) 182
BshNI GGYRCC 1 cut(s) 135
BsiHKAI GWGCWC 1 cut(s) 51
BsiWI CGTACG 1 cut(s) 6
BslI CCNNNNNNNGG 4 cut(s) 115, 189, 206, 310
BsmAI GTCTC 1 cut(s) 75
BsnI GGCC 1 cut(s) 182
Bsp1286I GDGCHC 3 cut(s) 51, 74, 140
Bsp143I GATC 4 cut(s) 21, 93, 126, 388
BspACI CCGC 5 cut(s) 78, 464, 485, 488, 491
BspANI GGCC 1 cut(s) 182
BspCNI CTCAG 1 cut(s) 254
BspFNI CGCG 1 cut(s) 466
BspLI GGNNCC 2 cut(s) 137, 373
BspT107I GGYRCC 1 cut(s) 135
BssECI CCNNGG 1 cut(s) 99
BssMI GATC 4 cut(s) 21, 93, 126, 388
BssT1I CCWWGG 1 cut(s) 99
Bst2UI CCWGG 1 cut(s) 53
Bst4CI ACNGT 2 cut(s) 161, 362
Bst6I CTCTTC 1 cut(s) 117
BstBAI YACGTR 1 cut(s) 63
BstDEI CTNAG 2 cut(s) 166, 241
BstFNI CGCG 1 cut(s) 466
BstKTI GATC 4 cut(s) 24, 96, 129, 391
BstMAI GTCTC 1 cut(s) 75
BstMBI GATC 4 cut(s) 21, 93, 126, 388
BstMWI GCNNNNNNNGC 1 cut(s) 482
BstNI CCWGG 1 cut(s) 53
BstSCI CCNGG 1 cut(s) 51
BstSLI GKGCMC 2 cut(s) 74, 140
BstUI CGCG 1 cut(s) 466
BstV1I GCAGC 2 cut(s) 208, 248
BstV2I GAAGAC 1 cut(s) 111
BsuRI GGCC 1 cut(s) 182
BtrI CACGTC 1 cut(s) 16
BtsI GCAGTG 1 cut(s) 393
BtsIMutI CAGTG 1 cut(s) 393
Csp6I GTAC 4 cut(s) 7, 58, 64, 470
CviAII CATG 1 cut(s) 75
CviQI GTAC 4 cut(s) 7, 58, 64, 470
DdeI CTNAG 2 cut(s) 166, 241
DpnI GATC 4 cut(s) 23, 95, 128, 390
DpnII GATC 4 cut(s) 21, 93, 126, 388
Eam1104I CTCTTC 1 cut(s) 117
EarI CTCTTC 1 cut(s) 117
EciI GGCGGA 1 cut(s) 506
Ecl136II GAGCTC 1 cut(s) 49
Eco130I CCWWGG 1 cut(s) 99
Eco24I GRGCYC 1 cut(s) 51
Eco53kI GAGCTC 1 cut(s) 49
Eco57I CTGAAG 1 cut(s) 242
EcoICRI GAGCTC 1 cut(s) 49
EcoRII CCWGG 1 cut(s) 51
EcoT14I CCWWGG 1 cut(s) 99
EcoT38I GRGCYC 1 cut(s) 51
ErhI CCWWGG 1 cut(s) 99
FaeI CATG 1 cut(s) 78
FaiI YATR 3 cut(s) 32, 76, 190
FatI CATG 1 cut(s) 74
Fnu4HI GCNGC 5 cut(s) 197, 262, 464, 486, 489
FriOI GRGCYC 1 cut(s) 51
Fsp4HI GCNGC 5 cut(s) 197, 262, 464, 486, 489
FspBI CTAG 3 cut(s) 141, 201, 520
GluI GCNGC 5 cut(s) 197, 262, 464, 486, 489
GsuI CTGGAG 1 cut(s) 35
HaeIII GGCC 1 cut(s) 182
Hin1II CATG 1 cut(s) 78
HindIII AAGCTT 1 cut(s) 474
HinfI GANTC 3 cut(s) 214, 350, 454
HphI GGTGA 3 cut(s) 88, 149, 259
Hpy166II GTNNAC 1 cut(s) 60
Hpy188I TCNGA 3 cut(s) 177, 222, 349
Hpy188III TCNNGA 3 cut(s) 91, 340, 365
Hpy8I GTNNAC 1 cut(s) 60
Hpy99I CGWCG 4 cut(s) 14, 17, 47, 165
HpyAV CCTTC 2 cut(s) 204, 346
HpyCH4III ACNGT 2 cut(s) 161, 362
HpyCH4IV ACGT 2 cut(s) 15, 62
HpyCH4V TGCA 2 cut(s) 256, 264
HpyF10VI GCNNNNNNNGC 1 cut(s) 482
HpyF3I CTNAG 2 cut(s) 166, 241
HpySE526I ACGT 2 cut(s) 15, 62
Hsp92II CATG 1 cut(s) 78
Kzo9I GATC 4 cut(s) 21, 93, 126, 388
LmnI GCTCC 2 cut(s) 54, 377
LpnPI CCDG 5 cut(s) 38, 65, 104, 122, 378
Lsp1109I GCAGC 2 cut(s) 208, 248
LweI GCATC 1 cut(s) 273
MaeI CTAG 3 cut(s) 141, 201, 520
MaeII ACGT 2 cut(s) 15, 62
MalI GATC 4 cut(s) 23, 95, 128, 390
MboI GATC 4 cut(s) 21, 93, 126, 388
MboII GAAGA 5 cut(s) 38, 116, 134, 209, 438
MhlI GDGCHC 3 cut(s) 51, 74, 140
MluCI AATT 2 cut(s) 113, 271
MlyI GAGTC 1 cut(s) 344
MmeI TCCRAC 1 cut(s) 381
MnlI CCTC 7 cut(s) 165, 186, 313, 318, 343, 364, 487
MseI TTAA 1 cut(s) 237
MspR9I CCNGG 1 cut(s) 53
MvaI CCWGG 1 cut(s) 53
MvnI CGCG 1 cut(s) 466
MwoI GCNNNNNNNGC 1 cut(s) 482
NdeII GATC 4 cut(s) 21, 93, 126, 388
NlaIII CATG 1 cut(s) 78
NlaIV GGNNCC 2 cut(s) 137, 373
NmeAIII GCCGAG 1 cut(s) 486
PcsI WCGNNNNNNNCGW 1 cut(s) 21
PfeI GAWTC 2 cut(s) 214, 454
Pfl23II CGTACG 1 cut(s) 6
PkrI GCNGC 5 cut(s) 198, 263, 465, 487, 490
PleI GAGTC 1 cut(s) 344
PpsI GAGTC 1 cut(s) 344
Ppu21I YACGTR 1 cut(s) 63
Psp124BI GAGCTC 1 cut(s) 51
Psp6I CCWGG 1 cut(s) 51
PspGI CCWGG 1 cut(s) 51
PspLI CGTACG 1 cut(s) 6
PspN4I GGNNCC 2 cut(s) 137, 373
RsaI GTAC 4 cut(s) 8, 59, 65, 471
RsaNI GTAC 4 cut(s) 7, 58, 64, 470
SacI GAGCTC 1 cut(s) 51
SaqAI TTAA 1 cut(s) 237
SatI GCNGC 5 cut(s) 197, 262, 464, 486, 489
Sau3AI GATC 4 cut(s) 21, 93, 126, 388
SchI GAGTC 1 cut(s) 344
ScrFI CCNGG 1 cut(s) 53
SduI GDGCHC 3 cut(s) 51, 74, 140
SetI ASST 6 cut(s) 18, 51, 65, 111, 329, 478
SfaNI GCATC 1 cut(s) 273
Sse9I AATT 2 cut(s) 113, 271
SsiI CCGC 5 cut(s) 78, 464, 485, 488, 491
SspMI CTAG 3 cut(s) 141, 201, 520
SstI GAGCTC 1 cut(s) 51
StyD4I CCNGG 1 cut(s) 51
StyI CCWWGG 1 cut(s) 99
TaaI ACNGT 2 cut(s) 161, 362
TaiI ACGT 2 cut(s) 18, 65
TaqI TCGA 3 cut(s) 24, 318, 479
TasI AATT 2 cut(s) 113, 271
TatI WGTACW 1 cut(s) 469
TauI GCSGC 3 cut(s) 466, 488, 491
TfiI GAWTC 2 cut(s) 214, 454
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
TscAI CASTG 1 cut(s) 400
TseI GCWGC 2 cut(s) 196, 261
TspRI CASTG 1 cut(s) 400
XspI CTAG 3 cut(s) 141, 201, 520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.