Prupe.8G112600_v2.0.a1

Diphthamide biosynthesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0002470
Physical Location & Seq
Forward (+)
14139245 .. 14141987
2743 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G112600.2

Sequence Viewer

Length: 249 bp
ATGTCGTACGACGATGTGGAGATCGAAGATATGGAGTGGAACAATGAGCTCCAGGCGTACACATACCCCTGTCCATGCGGGGACTTGTTTCAGATCACCAAGGAGGACCTGAAGTTGGGCGAAGAGATCGCTCGGTGCCCTAGTTGCTCCCTCTACATCACCGTCGTTTACAACGTCGAAGATTTCTTAGATAAGAAAAGTAACAAACCCATCGAGCCCTCCAAGCAGCAACCCATTACTGTCGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CSL

Protein Analysis

83

Amino Acids

9.45

Weight (kDa)

4.18

Isoelectric Point (pI)

66.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 135
AciI CCGC 1 cut(s) 78
AcuI CTGAAG 1 cut(s) 131
AfaI GTAC 2 cut(s) 8, 59
AfiI CCNNNNNNNGG 1 cut(s) 115
AjnI CCWGG 1 cut(s) 51
AluBI AGCT 1 cut(s) 49
AluI AGCT 1 cut(s) 49
Alw21I GWGCWC 1 cut(s) 51
ApeKI GCWGC 1 cut(s) 226
AspS9I GGNCC 1 cut(s) 106
AsuHPI GGTGA 2 cut(s) 88, 151
AvaII GGWCC 1 cut(s) 106
BaeGI GKGCMC 1 cut(s) 140
BanI GGYRCC 1 cut(s) 135
BanII GRGCYC 2 cut(s) 51, 219
Bbv12I GWGCWC 1 cut(s) 51
BbvI GCAGC 1 cut(s) 238
BccI CCATC 1 cut(s) 218
BciT130I CCWGG 1 cut(s) 53
BfaI CTAG 1 cut(s) 141
BisI GCNGC 1 cut(s) 227
BlsI GCNGC 1 cut(s) 228
Bme1390I CCNGG 1 cut(s) 53
Bme18I GGWCC 1 cut(s) 106
BmgT120I GGNCC 1 cut(s) 106
BmiI GGNNCC 1 cut(s) 137
BmrFI CCNGG 1 cut(s) 53
BpmI CTGGAG 1 cut(s) 35
BsaJI CCNNGG 1 cut(s) 99
Bsc4I CCNNNNNNNGG 1 cut(s) 115
BseBI CCWGG 1 cut(s) 53
BseDI CCNNGG 1 cut(s) 99
BseLI CCNNNNNNNGG 1 cut(s) 115
BseSI GKGCMC 1 cut(s) 140
BseXI GCAGC 1 cut(s) 238
BshNI GGYRCC 1 cut(s) 135
BsiHKAI GWGCWC 1 cut(s) 51
BsiWI CGTACG 1 cut(s) 6
BslFI GGGAC 1 cut(s) 95
BslI CCNNNNNNNGG 1 cut(s) 115
BsmFI GGGAC 1 cut(s) 95
Bsp1286I GDGCHC 3 cut(s) 51, 140, 219
Bsp143I GATC 3 cut(s) 21, 93, 126
BspACI CCGC 1 cut(s) 78
BspLI GGNNCC 1 cut(s) 137
BspT107I GGYRCC 1 cut(s) 135
BssECI CCNNGG 1 cut(s) 99
BssMI GATC 3 cut(s) 21, 93, 126
BssT1I CCWWGG 1 cut(s) 99
Bst2UI CCWGG 1 cut(s) 53
Bst4CI ACNGT 2 cut(s) 163, 241
Bst6I CTCTTC 1 cut(s) 117
BstDEI CTNAG 1 cut(s) 187
BstKTI GATC 3 cut(s) 24, 96, 129
BstMBI GATC 3 cut(s) 21, 93, 126
BstMWI GCNNNNNNNGC 2 cut(s) 144, 223
BstNI CCWGG 1 cut(s) 53
BstSCI CCNGG 1 cut(s) 51
BstSLI GKGCMC 1 cut(s) 140
BstV1I GCAGC 1 cut(s) 238
Cfr13I GGNCC 1 cut(s) 106
Csp6I GTAC 2 cut(s) 7, 58
CviAII CATG 1 cut(s) 75
CviJI RGCY 2 cut(s) 49, 217
CviKI_1 RGCY 2 cut(s) 49, 217
CviQI GTAC 2 cut(s) 7, 58
DdeI CTNAG 1 cut(s) 187
DpnI GATC 3 cut(s) 23, 95, 128
DpnII GATC 3 cut(s) 21, 93, 126
Eam1104I CTCTTC 1 cut(s) 117
EarI CTCTTC 1 cut(s) 117
Ecl136II GAGCTC 1 cut(s) 49
Eco130I CCWWGG 1 cut(s) 99
Eco24I GRGCYC 2 cut(s) 51, 219
Eco47I GGWCC 1 cut(s) 106
Eco53kI GAGCTC 1 cut(s) 49
Eco57I CTGAAG 1 cut(s) 131
EcoICRI GAGCTC 1 cut(s) 49
EcoO109I RGGNCCY 1 cut(s) 106
EcoRII CCWGG 1 cut(s) 51
EcoT14I CCWWGG 1 cut(s) 99
EcoT38I GRGCYC 2 cut(s) 51, 219
ErhI CCWWGG 1 cut(s) 99
FaeI CATG 1 cut(s) 78
FaiI YATR 3 cut(s) 32, 64, 76
FaqI GGGAC 1 cut(s) 95
FatI CATG 1 cut(s) 74
FauI CCCGC 1 cut(s) 71
Fnu4HI GCNGC 1 cut(s) 227
FriOI GRGCYC 2 cut(s) 51, 219
Fsp4HI GCNGC 1 cut(s) 227
FspBI CTAG 1 cut(s) 141
GluI GCNGC 1 cut(s) 227
GsuI CTGGAG 1 cut(s) 35
Hin1II CATG 1 cut(s) 78
HphI GGTGA 2 cut(s) 88, 151
Hpy166II GTNNAC 2 cut(s) 60, 169
Hpy188I TCNGA 1 cut(s) 93
Hpy8I GTNNAC 2 cut(s) 60, 169
Hpy99I CGWCG 3 cut(s) 14, 167, 179
HpyCH4III ACNGT 2 cut(s) 163, 241
HpyCH4IV ACGT 1 cut(s) 174
HpyF10VI GCNNNNNNNGC 2 cut(s) 144, 223
HpyF3I CTNAG 1 cut(s) 187
HpySE526I ACGT 1 cut(s) 174
Hsp92II CATG 1 cut(s) 78
Kzo9I GATC 3 cut(s) 21, 93, 126
LmnI GCTCC 2 cut(s) 54, 152
LpnPI CCDG 4 cut(s) 38, 65, 82, 122
Lsp1109I GCAGC 1 cut(s) 238
MaeI CTAG 1 cut(s) 141
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 1 cut(s) 200
MalI GATC 3 cut(s) 23, 95, 128
MboI GATC 3 cut(s) 21, 93, 126
MboII GAAGA 3 cut(s) 38, 134, 191
MhlI GDGCHC 3 cut(s) 51, 140, 219
MnlI CCTC 3 cut(s) 97, 161, 229
MseI TTAA 1 cut(s) 247
MspR9I CCNGG 1 cut(s) 53
MvaI CCWGG 1 cut(s) 53
MwoI GCNNNNNNNGC 2 cut(s) 144, 223
NdeII GATC 3 cut(s) 21, 93, 126
NlaIII CATG 1 cut(s) 78
NlaIV GGNNCC 1 cut(s) 137
PcsI WCGNNNNNNNCGW 1 cut(s) 171
Pfl23II CGTACG 1 cut(s) 6
PkrI GCNGC 1 cut(s) 228
PpuMI RGGWCCY 1 cut(s) 106
Psp124BI GAGCTC 1 cut(s) 51
Psp5II RGGWCCY 1 cut(s) 106
Psp6I CCWGG 1 cut(s) 51
PspGI CCWGG 1 cut(s) 51
PspLI CGTACG 1 cut(s) 6
PspN4I GGNNCC 1 cut(s) 137
PspPI GGNCC 1 cut(s) 106
PspPPI RGGWCCY 1 cut(s) 106
RsaI GTAC 2 cut(s) 8, 59
RsaNI GTAC 2 cut(s) 7, 58
SacI GAGCTC 1 cut(s) 51
SaqAI TTAA 1 cut(s) 247
SatI GCNGC 1 cut(s) 227
Sau3AI GATC 3 cut(s) 21, 93, 126
Sau96I GGNCC 1 cut(s) 106
ScrFI CCNGG 1 cut(s) 53
SduI GDGCHC 3 cut(s) 51, 140, 219
SetI ASST 3 cut(s) 51, 111, 177
SinI GGWCC 1 cut(s) 106
SsiI CCGC 1 cut(s) 78
SspMI CTAG 1 cut(s) 141
SstI GAGCTC 1 cut(s) 51
StyD4I CCNGG 1 cut(s) 51
StyI CCWWGG 1 cut(s) 99
TaaI ACNGT 2 cut(s) 163, 241
TaiI ACGT 1 cut(s) 177
TaqI TCGA 3 cut(s) 24, 177, 213
Tru1I TTAA 1 cut(s) 247
Tru9I TTAA 1 cut(s) 247
TseI GCWGC 1 cut(s) 226
VpaK11BI GGWCC 1 cut(s) 106
XspI CTAG 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.