Rw6G009490

Diphthamide biosynthesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0002470
Physical Location & Seq
Reverse (-)
15911670 .. 15911909
240 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G009490.1

Sequence Viewer

Length: 240 bp
ATGTCGTACGACGATGTGGAGATCGAAGATATGGAGTGGAATGACGAGCTCCAGGCGTACACATACCCATGCCCATGCGGAGACTTGTTCCAAATCACCAAGGAAGACCTGAAATTGGGGGAAGAGATCGCCCGGTGCCCTAGTTGTACTCTCTACATCACCGTCGTTTACAACATCGAAGACTTCATCGATAACAAGCCCTTCGAGCCCTCCAAGCAACAATCCCTCACCGTCGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CSL

Protein Analysis

79

Amino Acids

9.16

Weight (kDa)

4.05

Isoelectric Point (pI)

84.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_CSL PF05207 4 - 58 4.5e-23 CSL zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 135
AciI CCGC 1 cut(s) 78
AfaI GTAC 3 cut(s) 8, 59, 148
AfiI CCNNNNNNNGG 1 cut(s) 115
AjnI CCWGG 1 cut(s) 51
AluBI AGCT 1 cut(s) 49
AluI AGCT 1 cut(s) 49
Alw21I GWGCWC 1 cut(s) 51
Alw26I GTCTC 1 cut(s) 75
AsuC2I CCSGG 1 cut(s) 133
AsuHPI GGTGA 3 cut(s) 88, 151, 220
BaeGI GKGCMC 1 cut(s) 140
BanI GGYRCC 1 cut(s) 135
BanII GRGCYC 2 cut(s) 51, 210
BbsI GAAGAC 2 cut(s) 111, 186
Bbv12I GWGCWC 1 cut(s) 51
BciT130I CCWGG 1 cut(s) 53
BcnI CCSGG 1 cut(s) 133
BcoDI GTCTC 1 cut(s) 75
BfaI CTAG 1 cut(s) 141
Bme1390I CCNGG 2 cut(s) 53, 133
BmiI GGNNCC 1 cut(s) 137
BmrFI CCNGG 2 cut(s) 53, 133
BpiI GAAGAC 2 cut(s) 111, 186
BpmI CTGGAG 1 cut(s) 35
BpuMI CCSGG 1 cut(s) 133
Bsa29I ATCGAT 1 cut(s) 189
BsaJI CCNNGG 1 cut(s) 99
Bsc4I CCNNNNNNNGG 1 cut(s) 115
BseBI CCWGG 1 cut(s) 53
BseCI ATCGAT 1 cut(s) 189
BseDI CCNNGG 1 cut(s) 99
BseLI CCNNNNNNNGG 1 cut(s) 115
BseSI GKGCMC 1 cut(s) 140
BshNI GGYRCC 1 cut(s) 135
BshVI ATCGAT 1 cut(s) 189
BsiHKAI GWGCWC 1 cut(s) 51
BsiSI CCGG 1 cut(s) 133
BsiWI CGTACG 1 cut(s) 6
BslI CCNNNNNNNGG 1 cut(s) 115
BsmAI GTCTC 1 cut(s) 75
Bsp1286I GDGCHC 3 cut(s) 51, 140, 210
Bsp143I GATC 2 cut(s) 21, 126
BspACI CCGC 1 cut(s) 78
BspDI ATCGAT 1 cut(s) 189
BspLI GGNNCC 1 cut(s) 137
BspT107I GGYRCC 1 cut(s) 135
BssECI CCNNGG 1 cut(s) 99
BssMI GATC 2 cut(s) 21, 126
BssT1I CCWWGG 1 cut(s) 99
Bst2UI CCWGG 1 cut(s) 53
Bst4CI ACNGT 2 cut(s) 163, 232
Bst6I CTCTTC 1 cut(s) 117
BstDEI CTNAG 1 cut(s) 237
BstKTI GATC 2 cut(s) 24, 129
BstMAI GTCTC 1 cut(s) 75
BstMBI GATC 2 cut(s) 21, 126
BstMWI GCNNNNNNNGC 2 cut(s) 205, 214
BstNI CCWGG 1 cut(s) 53
BstSCI CCNGG 2 cut(s) 51, 131
BstSLI GKGCMC 1 cut(s) 140
BstV2I GAAGAC 2 cut(s) 111, 186
Bsu15I ATCGAT 1 cut(s) 189
BsuTUI ATCGAT 1 cut(s) 189
ClaI ATCGAT 1 cut(s) 189
Csp6I GTAC 3 cut(s) 7, 58, 147
CviAII CATG 2 cut(s) 69, 75
CviJI RGCY 3 cut(s) 49, 199, 208
CviKI_1 RGCY 3 cut(s) 49, 199, 208
CviQI GTAC 3 cut(s) 7, 58, 147
DdeI CTNAG 1 cut(s) 237
DpnI GATC 2 cut(s) 23, 128
DpnII GATC 2 cut(s) 21, 126
Eam1104I CTCTTC 1 cut(s) 117
EarI CTCTTC 1 cut(s) 117
Ecl136II GAGCTC 1 cut(s) 49
Eco130I CCWWGG 1 cut(s) 99
Eco24I GRGCYC 2 cut(s) 51, 210
Eco53kI GAGCTC 1 cut(s) 49
EcoICRI GAGCTC 1 cut(s) 49
EcoRII CCWGG 1 cut(s) 51
EcoT14I CCWWGG 1 cut(s) 99
EcoT38I GRGCYC 2 cut(s) 51, 210
ErhI CCWWGG 1 cut(s) 99
FaeI CATG 2 cut(s) 72, 78
FaiI YATR 4 cut(s) 32, 64, 70, 76
FatI CATG 2 cut(s) 68, 74
FriOI GRGCYC 2 cut(s) 51, 210
FspBI CTAG 1 cut(s) 141
GsuI CTGGAG 1 cut(s) 35
HapII CCGG 1 cut(s) 133
Hin1II CATG 2 cut(s) 72, 78
HpaII CCGG 1 cut(s) 133
HphI GGTGA 3 cut(s) 88, 151, 220
Hpy166II GTNNAC 2 cut(s) 60, 169
Hpy8I GTNNAC 2 cut(s) 60, 169
Hpy99I CGWCG 3 cut(s) 14, 167, 236
HpyAV CCTTC 1 cut(s) 211
HpyCH4III ACNGT 2 cut(s) 163, 232
HpyF10VI GCNNNNNNNGC 2 cut(s) 205, 214
HpyF3I CTNAG 1 cut(s) 237
Hsp92II CATG 2 cut(s) 72, 78
Kzo9I GATC 2 cut(s) 21, 126
LmnI GCTCC 1 cut(s) 54
LpnPI CCDG 4 cut(s) 38, 65, 122, 146
MaeI CTAG 1 cut(s) 141
MalI GATC 2 cut(s) 23, 128
MboI GATC 2 cut(s) 21, 126
MboII GAAGA 4 cut(s) 38, 116, 134, 191
MhlI GDGCHC 3 cut(s) 51, 140, 210
MluCI AATT 1 cut(s) 113
MnlI CCTC 2 cut(s) 220, 236
MslI CAYNNNNRTG 2 cut(s) 67, 73
MspI CCGG 1 cut(s) 133
MspR9I CCNGG 2 cut(s) 53, 133
MvaI CCWGG 1 cut(s) 53
MwoI GCNNNNNNNGC 2 cut(s) 205, 214
NciI CCSGG 1 cut(s) 133
NdeII GATC 2 cut(s) 21, 126
NlaIII CATG 2 cut(s) 72, 78
NlaIV GGNNCC 1 cut(s) 137
Pfl23II CGTACG 1 cut(s) 6
Psp124BI GAGCTC 1 cut(s) 51
Psp6I CCWGG 1 cut(s) 51
PspGI CCWGG 1 cut(s) 51
PspLI CGTACG 1 cut(s) 6
PspN4I GGNNCC 1 cut(s) 137
RsaI GTAC 3 cut(s) 8, 59, 148
RsaNI GTAC 3 cut(s) 7, 58, 147
RseI CAYNNNNRTG 2 cut(s) 67, 73
SacI GAGCTC 1 cut(s) 51
Sau3AI GATC 2 cut(s) 21, 126
ScrFI CCNGG 2 cut(s) 53, 133
SduI GDGCHC 3 cut(s) 51, 140, 210
SetI ASST 2 cut(s) 51, 111
SmiMI CAYNNNNRTG 2 cut(s) 67, 73
Sse9I AATT 1 cut(s) 113
SsiI CCGC 1 cut(s) 78
SspMI CTAG 1 cut(s) 141
SstI GAGCTC 1 cut(s) 51
StyD4I CCNGG 2 cut(s) 51, 131
StyI CCWWGG 1 cut(s) 99
TaaI ACNGT 2 cut(s) 163, 232
TaqI TCGA 4 cut(s) 24, 177, 189, 204
TasI AATT 1 cut(s) 113
TatI WGTACW 1 cut(s) 146
TspDTI ATGAA 1 cut(s) 175
XspI CTAG 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.