Rw6G009860

Diphthamide biosynthesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0002470
Physical Location & Seq
Forward (+)
16672330 .. 16672578
249 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G009860.1

Sequence Viewer

Length: 249 bp
ATGTCGTACGACGACGTGGAGATCAAAGATATGGAGTGGAACGACGAGCTCGAGGCGTACACATACCCATGCCCATGCGGAGACTTGTTCCAGATTACCAAGGAAGACCTGAAATTGGGGGAAGAGATTGCTCAGTGTCCTAGCTGTACTCTCTACATCACCGTCGTTTACAACATCGAAGATTTCATGGATAAGAAACCCACCAAACCCTTTGAGCCCTCCAAGCAACAATCCCTCACCGTCGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CSL

Protein Analysis

82

Amino Acids

9.49

Weight (kDa)

4.12

Isoelectric Point (pI)

63.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_CSL PF05207 4 - 58 4.2e-23 CSL zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 78
AfaI GTAC 3 cut(s) 8, 59, 148
AfiI CCNNNNNNNGG 1 cut(s) 115
AjiI CACGTC 1 cut(s) 16
AluBI AGCT 2 cut(s) 49, 144
AluI AGCT 2 cut(s) 49, 144
Alw21I GWGCWC 1 cut(s) 51
Alw26I GTCTC 1 cut(s) 75
Ama87I CYCGRG 1 cut(s) 50
AsuHPI GGTGA 2 cut(s) 151, 229
AvaI CYCGRG 1 cut(s) 50
BanII GRGCYC 2 cut(s) 51, 219
BbsI GAAGAC 1 cut(s) 111
Bbv12I GWGCWC 1 cut(s) 51
BcoDI GTCTC 1 cut(s) 75
BfaI CTAG 1 cut(s) 141
BmeT110I CYCGRG 1 cut(s) 50
BmgBI CACGTC 1 cut(s) 16
BpiI GAAGAC 1 cut(s) 111
BsaJI CCNNGG 1 cut(s) 99
Bsc4I CCNNNNNNNGG 1 cut(s) 115
BseDI CCNNGG 1 cut(s) 99
BseLI CCNNNNNNNGG 1 cut(s) 115
BseMII CTCAG 1 cut(s) 146
BsiHKAI GWGCWC 1 cut(s) 51
BsiHKCI CYCGRG 1 cut(s) 50
BsiWI CGTACG 1 cut(s) 6
BslI CCNNNNNNNGG 1 cut(s) 115
BsmAI GTCTC 1 cut(s) 75
BsoBI CYCGRG 1 cut(s) 50
Bsp1286I GDGCHC 2 cut(s) 51, 219
Bsp143I GATC 1 cut(s) 21
BspACI CCGC 1 cut(s) 78
BspCNI CTCAG 1 cut(s) 145
BssECI CCNNGG 1 cut(s) 99
BssMI GATC 1 cut(s) 21
BssT1I CCWWGG 1 cut(s) 99
Bst4CI ACNGT 2 cut(s) 163, 241
Bst6I CTCTTC 1 cut(s) 117
BstDEI CTNAG 2 cut(s) 132, 246
BstKTI GATC 1 cut(s) 24
BstMAI GTCTC 1 cut(s) 75
BstMBI GATC 1 cut(s) 21
BstMWI GCNNNNNNNGC 1 cut(s) 223
BstV2I GAAGAC 1 cut(s) 111
BtrI CACGTC 1 cut(s) 16
BtsIMutI CAGTG 1 cut(s) 140
Csp6I GTAC 3 cut(s) 7, 58, 147
CviAII CATG 3 cut(s) 69, 75, 187
CviJI RGCY 3 cut(s) 49, 144, 217
CviKI_1 RGCY 3 cut(s) 49, 144, 217
CviQI GTAC 3 cut(s) 7, 58, 147
DdeI CTNAG 2 cut(s) 132, 246
DpnI GATC 1 cut(s) 23
DpnII GATC 1 cut(s) 21
Eam1104I CTCTTC 1 cut(s) 117
EarI CTCTTC 1 cut(s) 117
Ecl136II GAGCTC 1 cut(s) 49
Eco130I CCWWGG 1 cut(s) 99
Eco24I GRGCYC 2 cut(s) 51, 219
Eco53kI GAGCTC 1 cut(s) 49
Eco88I CYCGRG 1 cut(s) 50
EcoICRI GAGCTC 1 cut(s) 49
EcoT14I CCWWGG 1 cut(s) 99
EcoT38I GRGCYC 2 cut(s) 51, 219
ErhI CCWWGG 1 cut(s) 99
FaeI CATG 3 cut(s) 72, 78, 190
FaiI YATR 5 cut(s) 32, 64, 70, 76, 188
FatI CATG 3 cut(s) 68, 74, 186
FriOI GRGCYC 2 cut(s) 51, 219
FspBI CTAG 1 cut(s) 141
Hin1II CATG 3 cut(s) 72, 78, 190
HphI GGTGA 2 cut(s) 151, 229
Hpy166II GTNNAC 2 cut(s) 60, 169
Hpy188III TCNNGA 1 cut(s) 91
Hpy8I GTNNAC 2 cut(s) 60, 169
Hpy99I CGWCG 5 cut(s) 14, 17, 47, 167, 245
HpyCH4III ACNGT 2 cut(s) 163, 241
HpyCH4IV ACGT 1 cut(s) 15
HpyF10VI GCNNNNNNNGC 1 cut(s) 223
HpyF3I CTNAG 2 cut(s) 132, 246
HpySE526I ACGT 1 cut(s) 15
Hsp92II CATG 3 cut(s) 72, 78, 190
Kzo9I GATC 1 cut(s) 21
LpnPI CCDG 2 cut(s) 104, 122
MaeI CTAG 1 cut(s) 141
MaeII ACGT 1 cut(s) 15
MalI GATC 1 cut(s) 23
MboI GATC 1 cut(s) 21
MboII GAAGA 3 cut(s) 116, 134, 191
MhlI GDGCHC 2 cut(s) 51, 219
MluCI AATT 1 cut(s) 113
MnlI CCTC 3 cut(s) 46, 229, 245
MslI CAYNNNNRTG 2 cut(s) 67, 73
MwoI GCNNNNNNNGC 1 cut(s) 223
NdeII GATC 1 cut(s) 21
NlaIII CATG 3 cut(s) 72, 78, 190
PaeR7I CTCGAG 1 cut(s) 50
PcsI WCGNNNNNNNCGW 1 cut(s) 48
Pfl23II CGTACG 1 cut(s) 6
Psp124BI GAGCTC 1 cut(s) 51
PspLI CGTACG 1 cut(s) 6
PspXI VCTCGAGB 1 cut(s) 50
RsaI GTAC 3 cut(s) 8, 59, 148
RsaNI GTAC 3 cut(s) 7, 58, 147
RseI CAYNNNNRTG 2 cut(s) 67, 73
SacI GAGCTC 1 cut(s) 51
Sau3AI GATC 1 cut(s) 21
SduI GDGCHC 2 cut(s) 51, 219
SetI ASST 4 cut(s) 18, 51, 111, 146
Sfr274I CTCGAG 1 cut(s) 50
SlaI CTCGAG 1 cut(s) 50
SmiMI CAYNNNNRTG 2 cut(s) 67, 73
SmlI CTYRAG 1 cut(s) 50
SmoI CTYRAG 1 cut(s) 50
Sse9I AATT 1 cut(s) 113
SsiI CCGC 1 cut(s) 78
SspMI CTAG 1 cut(s) 141
SstI GAGCTC 1 cut(s) 51
StyI CCWWGG 1 cut(s) 99
TaaI ACNGT 2 cut(s) 163, 241
TaiI ACGT 1 cut(s) 18
TaqI TCGA 2 cut(s) 51, 177
TasI AATT 1 cut(s) 113
TatI WGTACW 1 cut(s) 146
TscAI CASTG 1 cut(s) 140
TspDTI ATGAA 1 cut(s) 175
TspRI CASTG 1 cut(s) 140
XhoI CTCGAG 1 cut(s) 50
XspI CTAG 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.