Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0005279

Physical Location & Seq

Genomic Coordinates Forward (+)

30541114 .. 30546566

Gene Structure 5453 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1206500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1359 bp

ATGGCGGCTACCGACGAAGAGAAGGAGGCGGCGACGACGACGACGACAGCGACGACGACGTTTAAGAATTTGGGGATATGCGACGAATTGGTGGATGCTTGTGAGAAATTGGGGTGGACAATCCCTACCCCAATTCAAGTCGAATCCATGCCTGCCGCCCTCGAAGGAAAGGACTTGATCGCACTTGCACAAACGGGTTCGGGTAAAACCGGCGCATTCGCGCTTCCGATACTGCAAGAACTAATAAAGACTCCACAATCATTTTTCGCTTGCGTTCTGTCTCCAACGAGAGAGCTTGCAATTCAGATCGCCGAGCAGTTTGAAGCTTTGGGGTCCGAGATTGGTGTGAAGTGTGCAGTGCTTGTTGGAGGAGTGGACATGGTGCAACAATCTATCAATCTTGCAAAGCGGCCACATGTCGTTGTTGCAACACCTGGACGCCTCATGGATCATCTGTCCAACACGAAAGGATTTTCTCTTCGCACGTTGAAATTTTTGGTTTTAGATGAGGCTGACAGGTTGCTTAATGAGGACTTTGAAAAGTCAATTGATGAAATTCTGAATGAAATCCCTCGTGATCGGAGGACATACTTATTCTCTGCTACTATGACTAAAAAGGTTCGGAAGCTGCAAAGGGCTTGCCTAAGGAACCCTGTGAAGATTGAAGTAGCGTCTAAGTATTCGACAGTTGACACACTAAAGCAGCAGTATCGTTTTCTGCCTGCCAAGTACAAGGATTGCTATCTTGTATATATTCTGACAGAGAAGTCTCAGTGTACAACTATGGTATTCACCCGCACATGCGATGCAACACAAGTTTTGGCTTTGATGCTTCGAAATCTTGGCATAAGGGCCATCCCAATTAGTGGTCACATGAGCCAGTCAAAAAGGCTTGGAGCCTTAAATATGTTCAAAGCTGGTGAATGTAATGTACTTATCTGCACTGATGTGGCTAGTAGAGGATTGGATATTCCATCAGTGGATATGGTTATTAATTATGACATTCCCACAAATTCTAAGGATTATATCCATAGAGTTGGAAGGACTGCTCGTGCTGGACGATCTGGGGTTGCAATCTCTCTAGTGAACCAGTATGAGTTGGAGTGGTATATACAGATAGAGAAGCTTATCGGCAAAAAGTTACCCGAATTTTCTGCTCAAGAAGAGGAAGTCCTGCTTCTGTTAGAACGTGTCGGGCAGGCGAAAAGACTAGCCCAGATGAAAATTAAAGAGAGCGGTGGCAAGAAGAGGAAGGGGGGAAACAATGGTGAGGAGGAGGATATTGACAAGTACTTAGGTCACAAAGGAGGAGGAAAGTCCGGAGGAAAGTCGAAGAAGTTCAAAAAATCAAGAGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010467 GO:0010501 GO:0016070 GO:0016072 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022613 GO:0031974 GO:0031981 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

453

Amino Acids

50.02

Weight (kDa)

9.02

Isoelectric Point (pI)

39.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	43 - 209	5.8e-48	DEAD/DEAH box helicase
ResIII	PF04851	58 - 203	9.5e-06	Type III restriction enzyme, res subunit
Helicase_C	PF00271	248 - 353	3.8e-30	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005279)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G60990
fragaria_vesca	FvH4_3g15190 FvH4_3g15190 FvH4_3g15190
malus_domestica	MD10G1206500.v1.1
prunus_persica	Prupe.4G135800_v2.0.a1
pyrus_communis	pycom10g17630
rosa_chinensis	RchiOBHm_Chr2g0164771 RchiOBHm_Chr5g0025631
rosa_laevigata	RLG00000021484 RLG00000021488 RLG00000031602 RLG00000032853
rosa_multiflora	Rmu_sc0002137.1_g000001
rosa_roxburghii	Rroxscaffold_1G00053540 Rroxscaffold_1G00054150 Rroxscaffold_2G00083670 Rroxscaffold_2G00086310
rosa_rugosa	Rorug02G0510100 Rorug02G0529200 Rorug05G0087300
rosa_samantha	Rh2AG578400 Rh2BG589500 Rh2CG560100 Rh2DG599900 Rh5AG179100 Rh5BG178200 Rh5DG177400
rosa_wichuraiana	Rw5G016260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	766
AccB7I	CCANNNNNTGG	1 cut(s)	866
AccBSI	CCGCTC	1 cut(s)	1236
AccII	CGCG	1 cut(s)	221
AccIII	TCCGGA	1 cut(s)	1319
AciI	CCGC	6 cut(s)	5, 29, 156, 409, 796, 1236
AclWI	GGATC	1 cut(s)	456
AcoI	YGGCCR	1 cut(s)	410
AcsI	RAATTY	5 cut(s)	67, 491, 555, 1012, 1148
AcyI	GRCGYC	1 cut(s)	439
AfaI	GTAC	4 cut(s)	731, 778, 933, 1292
AfiI	CCNNNNNNNGG	1 cut(s)	866
AflIII	ACRYGT	2 cut(s)	415, 1189
AgsI	TTSAA	7 cut(s)	137, 323, 490, 539, 665, 913, 1342
AjnI	CCWGG	1 cut(s)	433
AleI	CACNNNNGTG	1 cut(s)	947
AluBI	AGCT	5 cut(s)	295, 326, 628, 917, 1126
AluI	AGCT	5 cut(s)	295, 326, 628, 917, 1126
Alw26I	GTCTC	2 cut(s)	285, 774
AlwI	GGATC	1 cut(s)	456
Aor13HI	TCCGGA	1 cut(s)	1319
AoxI	GGCC	2 cut(s)	410, 852
ApeKI	GCWGC	2 cut(s)	628, 703
ApoI	RAATTY	5 cut(s)	67, 491, 555, 1012, 1148
AseI	ATTAAT	1 cut(s)	993
Asp700I	GAANNNNTTC	1 cut(s)	1337
AspLEI	GCGC	2 cut(s)	215, 223
AspS9I	GGNCC	2 cut(s)	333, 852
AsuHPI	GGTGA	3 cut(s)	784, 932, 1280
AsuII	TTCGAA	1 cut(s)	835
AvaII	GGWCC	1 cut(s)	333
AxyI	CCTNAGG	1 cut(s)	644
BauI	CACGAG	2 cut(s)	573, 1050
BbvI	GCAGC	2 cut(s)	615, 715
BccI	CCATC	2 cut(s)	863, 982
BcgI	CGANNNNNNTGC	4 cut(s)	692, 726, 1136, 1170
BciT130I	CCWGG	1 cut(s)	435
BcoDI	GTCTC	2 cut(s)	285, 774
BfaI	CTAG	3 cut(s)	954, 1082, 1211
BisI	GCNGC	6 cut(s)	6, 30, 156, 410, 629, 704
BlsI	GCNGC	6 cut(s)	7, 31, 157, 411, 630, 705
BmcAI	AGTACT	1 cut(s)	1292
Bme1390I	CCNGG	1 cut(s)	435
Bme18I	GGWCC	1 cut(s)	333
BmgT120I	GGNCC	2 cut(s)	333, 852
BmiI	GGNNCC	3 cut(s)	334, 650, 898
BmrFI	CCNGG	1 cut(s)	435
BmsI	GCATC	3 cut(s)	85, 796, 819
Bpu14I	TTCGAA	1 cut(s)	835
BpuEI	CTTGAG	1 cut(s)	1143
BsaBI	GATNNNNATC	1 cut(s)	741
BsaHI	GRCGYC	1 cut(s)	439
BsaWI	WCCGGW	1 cut(s)	1319
Bsc4I	CCNNNNNNNGG	1 cut(s)	866
Bse118I	RCCGGY	1 cut(s)	209
Bse1I	ACTGG	2 cut(s)	880, 1090
Bse21I	CCTNAGG	1 cut(s)	644
Bse8I	GATNNNNATC	1 cut(s)	741
BseAI	TCCGGA	1 cut(s)	1319
BseBI	CCWGG	1 cut(s)	435
BseGI	GGATG	2 cut(s)	100, 855
BseJI	GATNNNNATC	1 cut(s)	741
BseLI	CCNNNNNNNGG	1 cut(s)	866
BseMII	CTCAG	1 cut(s)	785
BseNI	ACTGG	2 cut(s)	880, 1090
BseRI	GAGGAG	4 cut(s)	384, 1286, 1289, 1323
BseXI	GCAGC	2 cut(s)	615, 715
BsgI	GTGCAG	2 cut(s)	375, 925
Bsh1236I	CGCG	1 cut(s)	221
BshFI	GGCC	2 cut(s)	412, 854
BsiSI	CCGG	2 cut(s)	210, 1320
BslI	CCNNNNNNNGG	1 cut(s)	866
BsmAI	GTCTC	2 cut(s)	285, 774
BsmI	GAATGC	1 cut(s)	215
BsnI	GGCC	2 cut(s)	412, 854
Bsp119I	TTCGAA	1 cut(s)	835
Bsp13I	TCCGGA	1 cut(s)	1319
Bsp1407I	TGTACA	1 cut(s)	776
Bsp143I	GATC	5 cut(s)	177, 306, 448, 577, 1061
BspACI	CCGC	6 cut(s)	5, 29, 156, 409, 796, 1236
BspANI	GGCC	2 cut(s)	412, 854
BspCNI	CTCAG	1 cut(s)	784
BspEI	TCCGGA	1 cut(s)	1319
BspFNI	CGCG	1 cut(s)	221
BspLI	GGNNCC	3 cut(s)	334, 650, 898
BspPI	GGATC	1 cut(s)	456
BspT104I	TTCGAA	1 cut(s)	835
BsrBI	CCGCTC	1 cut(s)	1236
BsrFI	RCCGGY	1 cut(s)	209
BsrGI	TGTACA	1 cut(s)	776
BsrI	ACTGG	2 cut(s)	880, 1090
BssAI	RCCGGY	1 cut(s)	209
BssMI	GATC	5 cut(s)	177, 306, 448, 577, 1061
BssNI	GRCGYC	1 cut(s)	439
BssSI	CACGAG	2 cut(s)	573, 1050
Bst2BI	CACGAG	2 cut(s)	573, 1050
Bst2UI	CCWGG	1 cut(s)	435
Bst4CI	ACNGT	1 cut(s)	688
Bst6I	CTCTTC	4 cut(s)	12, 483, 1158, 1241
BstACI	GRCGYC	1 cut(s)	439
BstAUI	TGTACA	1 cut(s)	776
BstBI	TTCGAA	1 cut(s)	835
BstC8I	GCNNGC	6 cut(s)	153, 271, 297, 640, 723, 1200
BstDEI	CTNAG	5 cut(s)	644, 675, 771, 1017, 1294
BstF5I	GGATG	2 cut(s)	100, 855
BstFNI	CGCG	1 cut(s)	221
BstHHI	GCGC	2 cut(s)	215, 223
BstKTI	GATC	5 cut(s)	180, 309, 451, 580, 1064
BstMAI	GTCTC	2 cut(s)	285, 774
BstMBI	GATC	5 cut(s)	177, 306, 448, 577, 1061
BstNI	CCWGG	1 cut(s)	435
BstNSI	RCATGY	2 cut(s)	419, 804
BstSCI	CCNGG	1 cut(s)	433
BstUI	CGCG	1 cut(s)	221
BstV1I	GCAGC	2 cut(s)	615, 715
BstXI	CCANNNNNNTGG	1 cut(s)	1037
Bsu36I	CCTNAGG	1 cut(s)	644
BsuRI	GGCC	2 cut(s)	412, 854
BtgZI	GCGATG	1 cut(s)	819
BtsCI	GGATG	2 cut(s)	100, 855
BtsI	GCAGTG	1 cut(s)	363
BtsIMutI	CAGTG	4 cut(s)	363, 779, 942, 984
Cac8I	GCNNGC	6 cut(s)	153, 271, 297, 640, 723, 1200
CfoI	GCGC	2 cut(s)	215, 223
Cfr10I	RCCGGY	1 cut(s)	209
Cfr13I	GGNCC	2 cut(s)	333, 852
CseI	GACGC	2 cut(s)	447, 660
Csp6I	GTAC	4 cut(s)	730, 777, 932, 1291
CviAII	CATG	6 cut(s)	148, 379, 416, 445, 801, 874
CviQI	GTAC	4 cut(s)	730, 777, 932, 1291
DdeI	CTNAG	5 cut(s)	644, 675, 771, 1017, 1294
DpnI	GATC	5 cut(s)	179, 308, 450, 579, 1063
DpnII	GATC	5 cut(s)	177, 306, 448, 577, 1061
DrdI	GACNNNNNNGTC	1 cut(s)	766
DseDI	GACNNNNNNGTC	1 cut(s)	766
EaeI	YGGCCR	1 cut(s)	410
Eam1104I	CTCTTC	4 cut(s)	12, 483, 1158, 1241
EarI	CTCTTC	4 cut(s)	12, 483, 1158, 1241
Eco47I	GGWCC	1 cut(s)	333
Eco81I	CCTNAGG	1 cut(s)	644
EcoRII	CCWGG	1 cut(s)	433
FaeI	CATG	6 cut(s)	151, 382, 419, 448, 804, 877
FalI	AAGNNNNNCTT	2 cut(s)	1161, 1193
FatI	CATG	6 cut(s)	147, 378, 415, 444, 800, 873
FauI	CCCGC	1 cut(s)	803
Fnu4HI	GCNGC	6 cut(s)	6, 30, 156, 410, 629, 704
FokI	GGATG	2 cut(s)	107, 842
Fsp4HI	GCNGC	6 cut(s)	6, 30, 156, 410, 629, 704
FspBI	CTAG	3 cut(s)	954, 1082, 1211
GlaI	GCGC	2 cut(s)	214, 222
GluI	GCNGC	6 cut(s)	6, 30, 156, 410, 629, 704
HaeIII	GGCC	2 cut(s)	412, 854
HapII	CCGG	2 cut(s)	210, 1320
HgaI	GACGC	2 cut(s)	447, 660
HhaI	GCGC	2 cut(s)	215, 223
Hin1I	GRCGYC	1 cut(s)	439
Hin1II	CATG	6 cut(s)	151, 382, 419, 448, 804, 877
Hin6I	GCGC	2 cut(s)	213, 221
HinP1I	GCGC	2 cut(s)	213, 221
HincII	GTYRAC	1 cut(s)	691
HindII	GTYRAC	1 cut(s)	691
HindIII	AAGCTT	2 cut(s)	324, 1124
HinfI	GANTC	2 cut(s)	143, 250
HpaII	CCGG	2 cut(s)	210, 1320
HphI	GGTGA	3 cut(s)	784, 932, 1280
Hpy166II	GTNNAC	5 cut(s)	117, 376, 691, 777, 1087
Hpy188I	TCNGA	7 cut(s)	228, 306, 337, 561, 582, 624, 759
Hpy188III	TCNNGA	4 cut(s)	575, 1160, 1320, 1350
Hpy8I	GTNNAC	5 cut(s)	117, 376, 691, 777, 1087
Hpy99I	CGWCG	9 cut(s)	17, 37, 40, 43, 46, 55, 58, 61, 86
HpyAV	CCTTC	4 cut(s)	16, 158, 1035, 1246
HpyCH4III	ACNGT	1 cut(s)	688
HpyCH4IV	ACGT	3 cut(s)	59, 485, 1189
HpyF3I	CTNAG	5 cut(s)	644, 675, 771, 1017, 1294
HpySE526I	ACGT	3 cut(s)	59, 485, 1189
Hsp92I	GRCGYC	1 cut(s)	439
Hsp92II	CATG	6 cut(s)	151, 382, 419, 448, 804, 877
HspAI	GCGC	2 cut(s)	213, 221
Kpn2I	TCCGGA	1 cut(s)	1319
Kzo9I	GATC	5 cut(s)	177, 306, 448, 577, 1061
LmnI	GCTCC	1 cut(s)	896
Lsp1109I	GCAGC	2 cut(s)	615, 715
LweI	GCATC	3 cut(s)	85, 796, 819
MaeI	CTAG	3 cut(s)	954, 1082, 1211
MaeII	ACGT	3 cut(s)	59, 485, 1189
MaeIII	GTNAC	3 cut(s)	869, 1140, 1298
MalI	GATC	5 cut(s)	179, 308, 450, 579, 1063
MbiI	CCGCTC	1 cut(s)	1236
MboI	GATC	5 cut(s)	177, 306, 448, 577, 1061
MboII	GAAGA	6 cut(s)	29, 470, 670, 1175, 1258, 1345
MfeI	CAATTG	1 cut(s)	546
MlyI	GAGTC	1 cut(s)	244
MmeI	TCCRAC	5 cut(s)	308, 346, 483, 1018, 1080
MroI	TCCGGA	1 cut(s)	1319
MroXI	GAANNNNTTC	1 cut(s)	1337
MseI	TTAA	5 cut(s)	63, 525, 902, 993, 1227
MslI	CAYNNNNRTG	1 cut(s)	947
MspI	CCGG	2 cut(s)	210, 1320
MspR9I	CCNGG	1 cut(s)	435
MunI	CAATTG	1 cut(s)	546
Mva1269I	GAATGC	1 cut(s)	215
MvaI	CCWGG	1 cut(s)	435
MvnI	CGCG	1 cut(s)	221
NdeII	GATC	5 cut(s)	177, 306, 448, 577, 1061
NlaIII	CATG	6 cut(s)	151, 382, 419, 448, 804, 877
NlaIV	GGNNCC	3 cut(s)	334, 650, 898
NmeAIII	GCCGAG	1 cut(s)	337
NmuCI	GTSAC	2 cut(s)	869, 1298
NspI	RCATGY	2 cut(s)	419, 804
NspV	TTCGAA	1 cut(s)	835
OliI	CACNNNNGTG	1 cut(s)	947
PciI	ACATGT	1 cut(s)	415
PcsI	WCGNNNNNNNCGW	4 cut(s)	41, 47, 50, 1057
PctI	GAATGC	1 cut(s)	215
PdmI	GAANNNNTTC	1 cut(s)	1337
PfeI	GAWTC	1 cut(s)	143
PflMI	CCANNNNNTGG	1 cut(s)	866
PkrI	GCNGC	6 cut(s)	7, 31, 157, 411, 630, 705
PleI	GAGTC	1 cut(s)	244
PpsI	GAGTC	1 cut(s)	244
PscI	ACATGT	1 cut(s)	415
PshBI	ATTAAT	1 cut(s)	993
Psp6I	CCWGG	1 cut(s)	433
PspGI	CCWGG	1 cut(s)	433
PspN4I	GGNNCC	3 cut(s)	334, 650, 898
PspPI	GGNCC	2 cut(s)	333, 852
RsaI	GTAC	4 cut(s)	731, 778, 933, 1292
RsaNI	GTAC	4 cut(s)	730, 777, 932, 1291
RseI	CAYNNNNRTG	1 cut(s)	947
SaqAI	TTAA	5 cut(s)	63, 525, 902, 993, 1227
SatI	GCNGC	6 cut(s)	6, 30, 156, 410, 629, 704
Sau3AI	GATC	5 cut(s)	177, 306, 448, 577, 1061
Sau96I	GGNCC	2 cut(s)	333, 852
ScaI	AGTACT	1 cut(s)	1292
SchI	GAGTC	1 cut(s)	244
ScrFI	CCNGG	1 cut(s)	435
SfaNI	GCATC	3 cut(s)	85, 796, 819
SfuI	TTCGAA	1 cut(s)	835
SinI	GGWCC	1 cut(s)	333
SmiMI	CAYNNNNRTG	1 cut(s)	947
SmlI	CTYRAG	1 cut(s)	1158
SmoI	CTYRAG	1 cut(s)	1158
SsiI	CCGC	6 cut(s)	5, 29, 156, 409, 796, 1236
SspMI	CTAG	3 cut(s)	954, 1082, 1211
StyD4I	CCNGG	1 cut(s)	433
TaaI	ACNGT	1 cut(s)	688
TaiI	ACGT	3 cut(s)	62, 488, 1192
TaqI	TCGA	5 cut(s)	141, 162, 683, 835, 1331
TatI	WGTACW	4 cut(s)	729, 776, 931, 1290
TauI	GCSGC	4 cut(s)	8, 32, 158, 412
TfiI	GAWTC	1 cut(s)	143
Tru1I	TTAA	5 cut(s)	63, 525, 902, 993, 1227
Tru9I	TTAA	5 cut(s)	63, 525, 902, 993, 1227
TscAI	CASTG	4 cut(s)	363, 779, 949, 984
TseFI	GTSAC	2 cut(s)	869, 1298
TseI	GCWGC	2 cut(s)	628, 703
Tsp45I	GTSAC	2 cut(s)	869, 1298
TspDTI	ATGAA	3 cut(s)	567, 579, 1235
TspRI	CASTG	4 cut(s)	363, 779, 949, 984
Van91I	CCANNNNNTGG	1 cut(s)	866
VpaK11BI	GGWCC	1 cut(s)	333
VspI	ATTAAT	1 cut(s)	993
XapI	RAATTY	5 cut(s)	67, 491, 555, 1012, 1148
XceI	RCATGY	2 cut(s)	419, 804
XmnI	GAANNNNTTC	1 cut(s)	1337
XspI	CTAG	3 cut(s)	954, 1082, 1211
ZrmI	AGTACT	1 cut(s)	1292

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.