Rroxscaffold_2G00086310

Peptidase, trypsin-like serine and cysteine proteases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005279
Physical Location & Seq
Reverse (-)
8549446 .. 8551969
2524 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00086310.1

Sequence Viewer

Length: 882 bp
ATGGCCGCCGTCGAAAGAGAGGAGGAGGAGCTTAACACCCTTAACCAGAAGTTTTGTTTTCTGCTGGACAAGTACAAGGATTGCTACCTGGTATATATTCTTACTGAGAAGCGCCAATCTACAGCAATTGTTTTTGCTCGTTCACCAGATGTATGCAGATTGGCTTTAATGCTTCAATATCTTGGCATAAGGGCTATCCCAATTACTGGACATATGGCAGAGTCGCAGAGACTTCTTGCCTCAAGTATGTTCAAAGCTGGACAGTTTAATGTACTTATATGCACGGATATGGCAATTAGAGATTTGGATATTCCATCAGTCCATATTATTATTAGTTATGACATCCCCTCAAATCCTCAGGCAAAGAGTTACCTCAATTTCCTGCTCAAGAAGAGGAAGTCCTACAATTTCTTGAACCTGTCAGAGAAGCCAAAATATTGGCCGAATCAATCTCAGTTTAAAGCAGCTTGTCAGCTGAAAACCCTAGGTCGCAGACTCCTCTTCTCCACCGATGTCCCTCAACTCGAATCCCTAGACTTGGATGCTCCGTCGTTTGGAGACATTTCCTCCGTCAGGAAGCTCAGTTTCCCTCTCAAGGAACCGGCCTTTGATGTCAAACTACTGGGCTCGTGCAATGGTTTGGTGTTCGTAGCGGTTGTAGTCTGTGATGATTTCAAGCTTTACATCTGGAACCCATTAATGGGATTCTTCAAGGAGTTGCCCCAGCCAGGTTTGTCTACCCTGCCGGTCTATTACGGTGTTGGCTATTTGTCTGCCACCGACGACTACAAACTTTTCGCAGGCTCTTATGGGAAAGAGATCGAGATGTTATCCTTGAGAGCTCATGTTTGGAAAACAATCCAAAACCCTGGCTATAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

293

Amino Acids

33.31

Weight (kDa)

8.54

Isoelectric Point (pI)

40.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 29 - 120 2e-13 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 206
AccI GTMKAC 1 cut(s) 737
AciI CCGC 2 cut(s) 6, 653
AcoI YGGCCR 2 cut(s) 3, 440
AfaI GTAC 2 cut(s) 74, 273
AfiI CCNNNNNNNGG 8 cut(s) 206, 538, 554, 573, 595, 700, 701, 728
AgsI TTSAA 5 cut(s) 176, 253, 415, 676, 712
AjnI CCWGG 3 cut(s) 87, 727, 868
AluBI AGCT 7 cut(s) 31, 257, 467, 475, 580, 679, 842
AluI AGCT 7 cut(s) 31, 257, 467, 475, 580, 679, 842
Alw21I GWGCWC 1 cut(s) 844
Alw26I GTCTC 2 cut(s) 223, 552
AoxI GGCC 3 cut(s) 3, 440, 603
ApeKI GCWGC 1 cut(s) 464
AseI ATTAAT 1 cut(s) 698
AspA2I CCTAGG 1 cut(s) 484
AspLEI GCGC 1 cut(s) 114
AsuHPI GGTGA 1 cut(s) 135
AvrII CCTAGG 1 cut(s) 484
AxyI CCTNAGG 1 cut(s) 357
BanII GRGCYC 2 cut(s) 629, 844
BauI CACGAG 1 cut(s) 628
Bbv12I GWGCWC 1 cut(s) 844
BbvI GCAGC 1 cut(s) 476
BccI CCATC 1 cut(s) 322
BciT130I CCWGG 3 cut(s) 89, 729, 870
BcoDI GTCTC 2 cut(s) 223, 552
BfaI CTAG 2 cut(s) 485, 533
BfmI CTRYAG 1 cut(s) 120
BfoI RGCGCY 1 cut(s) 115
BisI GCNGC 2 cut(s) 6, 465
BlnI CCTAGG 1 cut(s) 484
BlsI GCNGC 2 cut(s) 7, 466
Bme1390I CCNGG 3 cut(s) 89, 729, 870
BmiI GGNNCC 2 cut(s) 600, 692
BmrFI CCNGG 3 cut(s) 89, 729, 870
BmrI ACTGGG 1 cut(s) 632
BmsI GCATC 1 cut(s) 532
BmuI ACTGGG 1 cut(s) 632
BpuEI CTTGAG 4 cut(s) 226, 371, 578, 856
BsaJI CCNNGG 2 cut(s) 484, 868
BsaXI ACNNNNNCTCC 2 cut(s) 551, 581
Bsc4I CCNNNNNNNGG 8 cut(s) 206, 538, 554, 573, 595, 700, 701, 728
Bse118I RCCGGY 2 cut(s) 601, 745
Bse1I ACTGG 2 cut(s) 211, 627
Bse21I CCTNAGG 1 cut(s) 357
Bse3DI GCAATG 1 cut(s) 640
BseBI CCWGG 3 cut(s) 89, 729, 870
BseDI CCNNGG 2 cut(s) 484, 868
BseGI GGATG 2 cut(s) 342, 547
BseLI CCNNNNNNNGG 8 cut(s) 206, 538, 554, 573, 595, 700, 701, 728
BseMI GCAATG 1 cut(s) 640
BseMII CTCAG 4 cut(s) 96, 371, 467, 595
BseNI ACTGG 2 cut(s) 211, 627
BseRI GAGGAG 4 cut(s) 35, 38, 41, 488
BseXI GCAGC 1 cut(s) 476
BseYI CCCAGC 1 cut(s) 723
BshFI GGCC 3 cut(s) 5, 442, 605
BsiHKAI GWGCWC 1 cut(s) 844
BsiSI CCGG 2 cut(s) 602, 746
BslFI GGGAC 1 cut(s) 500
BslI CCNNNNNNNGG 8 cut(s) 206, 538, 554, 573, 595, 700, 701, 728
BsmAI GTCTC 2 cut(s) 223, 552
BsmFI GGGAC 1 cut(s) 500
BsnI GGCC 3 cut(s) 5, 442, 605
Bsp1286I GDGCHC 2 cut(s) 629, 844
Bsp143I GATC 1 cut(s) 819
BspACI CCGC 2 cut(s) 6, 653
BspANI GGCC 3 cut(s) 5, 442, 605
BspCNI CTCAG 4 cut(s) 97, 370, 466, 594
BspLI GGNNCC 2 cut(s) 600, 692
BsrDI GCAATG 1 cut(s) 640
BsrFI RCCGGY 2 cut(s) 601, 745
BsrI ACTGG 2 cut(s) 211, 627
BssAI RCCGGY 2 cut(s) 601, 745
BssECI CCNNGG 2 cut(s) 484, 868
BssMI GATC 1 cut(s) 819
BssSI CACGAG 1 cut(s) 628
BssT1I CCWWGG 1 cut(s) 484
Bst2BI CACGAG 1 cut(s) 628
Bst2UI CCWGG 3 cut(s) 89, 729, 870
Bst4CI ACNGT 2 cut(s) 264, 758
Bst6I CTCTTC 2 cut(s) 386, 506
BstC8I GCNNGC 1 cut(s) 802
BstDEI CTNAG 4 cut(s) 105, 357, 453, 581
BstENI CCTNNNNNAGG 1 cut(s) 571
BstF5I GGATG 2 cut(s) 342, 547
BstH2I RGCGCY 1 cut(s) 115
BstHHI GCGC 1 cut(s) 114
BstKTI GATC 1 cut(s) 822
BstMAI GTCTC 2 cut(s) 223, 552
BstMBI GATC 1 cut(s) 819
BstNI CCWGG 3 cut(s) 89, 729, 870
BstSCI CCNGG 3 cut(s) 87, 727, 868
BstSFI CTRYAG 1 cut(s) 120
BstV1I GCAGC 1 cut(s) 476
BstXI CCANNNNNNTGG 2 cut(s) 438, 869
Bsu36I CCTNAGG 1 cut(s) 357
BsuRI GGCC 3 cut(s) 5, 442, 605
BtsCI GGATG 2 cut(s) 342, 547
Cac8I GCNNGC 1 cut(s) 802
CfoI GCGC 1 cut(s) 114
Cfr10I RCCGGY 2 cut(s) 601, 745
CsiI ACCWGGT 1 cut(s) 87
Csp6I GTAC 2 cut(s) 73, 272
CviAII CATG 1 cut(s) 845
CviQI GTAC 2 cut(s) 73, 272
DdeI CTNAG 4 cut(s) 105, 357, 453, 581
DpnI GATC 1 cut(s) 821
DpnII GATC 1 cut(s) 819
DraI TTTAAA 1 cut(s) 460
EaeI YGGCCR 2 cut(s) 3, 440
Eam1104I CTCTTC 2 cut(s) 386, 506
EarI CTCTTC 2 cut(s) 386, 506
Ecl136II GAGCTC 1 cut(s) 842
Eco130I CCWWGG 1 cut(s) 484
Eco24I GRGCYC 2 cut(s) 629, 844
Eco53kI GAGCTC 1 cut(s) 842
Eco81I CCTNAGG 1 cut(s) 357
EcoICRI GAGCTC 1 cut(s) 842
EcoNI CCTNNNNNAGG 1 cut(s) 571
EcoRII CCWGG 3 cut(s) 87, 727, 868
EcoT14I CCWWGG 1 cut(s) 484
EcoT38I GRGCYC 2 cut(s) 629, 844
ErhI CCWWGG 1 cut(s) 484
FaeI CATG 1 cut(s) 848
FaqI GGGAC 1 cut(s) 500
FatI CATG 1 cut(s) 844
FauNDI CATATG 1 cut(s) 213
FblI GTMKAC 1 cut(s) 737
Fnu4HI GCNGC 2 cut(s) 6, 465
FokI GGATG 2 cut(s) 329, 554
FriOI GRGCYC 2 cut(s) 629, 844
Fsp4HI GCNGC 2 cut(s) 6, 465
FspBI CTAG 2 cut(s) 485, 533
GlaI GCGC 1 cut(s) 113
GluI GCNGC 2 cut(s) 6, 465
GsaI CCCAGC 1 cut(s) 727
HaeII RGCGCY 1 cut(s) 115
HaeIII GGCC 3 cut(s) 5, 442, 605
HapII CCGG 2 cut(s) 602, 746
HhaI GCGC 1 cut(s) 114
Hin1II CATG 1 cut(s) 848
Hin6I GCGC 1 cut(s) 112
HinP1I GCGC 1 cut(s) 112
HindIII AAGCTT 1 cut(s) 677
HinfI GANTC 5 cut(s) 221, 445, 495, 527, 705
HpaII CCGG 2 cut(s) 602, 746
HphI GGTGA 1 cut(s) 135
Hpy166II GTNNAC 2 cut(s) 143, 738
Hpy188I TCNGA 1 cut(s) 424
Hpy188III TCNNGA 5 cut(s) 388, 412, 574, 688, 823
Hpy8I GTNNAC 2 cut(s) 143, 738
Hpy99I CGWCG 3 cut(s) 14, 553, 785
HpyCH4III ACNGT 2 cut(s) 264, 758
HpyCH4V TGCA 3 cut(s) 156, 282, 633
HpyF3I CTNAG 4 cut(s) 105, 357, 453, 581
Hsp92II CATG 1 cut(s) 848
HspAI GCGC 1 cut(s) 112
Kzo9I GATC 1 cut(s) 819
LmnI GCTCC 2 cut(s) 28, 550
Lsp1109I GCAGC 1 cut(s) 476
LweI GCATC 1 cut(s) 532
MabI ACCWGGT 1 cut(s) 87
MaeI CTAG 2 cut(s) 485, 533
MaeIII GTNAC 1 cut(s) 368
MalI GATC 1 cut(s) 821
MboI GATC 1 cut(s) 819
MboII GAAGA 3 cut(s) 403, 493, 700
MfeI CAATTG 1 cut(s) 126
MhlI GDGCHC 2 cut(s) 629, 844
MluCI AATT 5 cut(s) 126, 201, 294, 376, 406
MlyI GAGTC 2 cut(s) 230, 489
MseI TTAA 6 cut(s) 33, 42, 167, 267, 459, 698
MslI CAYNNNNRTG 1 cut(s) 287
MspA1I CMGCKG 1 cut(s) 475
MspI CCGG 2 cut(s) 602, 746
MspR9I CCNGG 3 cut(s) 89, 729, 870
MunI CAATTG 1 cut(s) 126
MvaI CCWGG 3 cut(s) 89, 729, 870
NdeI CATATG 1 cut(s) 213
NdeII GATC 1 cut(s) 819
NlaIII CATG 1 cut(s) 848
NlaIV GGNNCC 2 cut(s) 600, 692
PfeI GAWTC 3 cut(s) 445, 527, 705
PflMI CCANNNNNTGG 1 cut(s) 206
PkrI GCNGC 2 cut(s) 7, 466
PleI GAGTC 2 cut(s) 229, 489
PpsI GAGTC 2 cut(s) 229, 489
PshBI ATTAAT 1 cut(s) 698
Psp124BI GAGCTC 1 cut(s) 844
Psp6I CCWGG 3 cut(s) 87, 727, 868
PspFI CCCAGC 1 cut(s) 723
PspGI CCWGG 3 cut(s) 87, 727, 868
PspN4I GGNNCC 2 cut(s) 600, 692
PvuII CAGCTG 1 cut(s) 475
RsaI GTAC 2 cut(s) 74, 273
RsaNI GTAC 2 cut(s) 73, 272
RseI CAYNNNNRTG 1 cut(s) 287
SacI GAGCTC 1 cut(s) 844
SaqAI TTAA 6 cut(s) 33, 42, 167, 267, 459, 698
SatI GCNGC 2 cut(s) 6, 465
Sau3AI GATC 1 cut(s) 819
SchI GAGTC 2 cut(s) 230, 489
ScrFI CCNGG 3 cut(s) 89, 729, 870
SduI GDGCHC 2 cut(s) 629, 844
SexAI ACCWGGT 1 cut(s) 87
SfaNI GCATC 1 cut(s) 532
SfcI CTRYAG 1 cut(s) 120
SmiMI CAYNNNNRTG 1 cut(s) 287
SmlI CTYRAG 4 cut(s) 241, 386, 593, 835
SmoI CTYRAG 4 cut(s) 241, 386, 593, 835
Sse9I AATT 5 cut(s) 126, 201, 294, 376, 406
SsiI CCGC 2 cut(s) 6, 653
SspI AATATT 1 cut(s) 437
SspMI CTAG 2 cut(s) 485, 533
SstI GAGCTC 1 cut(s) 844
StyD4I CCNGG 3 cut(s) 87, 727, 868
StyI CCWWGG 1 cut(s) 484
TaaI ACNGT 2 cut(s) 264, 758
TaqI TCGA 3 cut(s) 12, 525, 822
TasI AATT 5 cut(s) 126, 201, 294, 376, 406
TatI WGTACW 2 cut(s) 72, 271
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 3 cut(s) 445, 527, 705
Tru1I TTAA 6 cut(s) 33, 42, 167, 267, 459, 698
Tru9I TTAA 6 cut(s) 33, 42, 167, 267, 459, 698
TseI GCWGC 1 cut(s) 464
TspGWI ACGGA 3 cut(s) 299, 537, 559
Van91I CCANNNNNTGG 1 cut(s) 206
VspI ATTAAT 1 cut(s) 698
XagI CCTNNNNNAGG 1 cut(s) 571
XmaJI CCTAGG 1 cut(s) 484
XmiI GTMKAC 1 cut(s) 737
XspI CTAG 2 cut(s) 485, 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.