Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0005279

Physical Location & Seq

Genomic Coordinates Reverse (-)

20012220 .. 20017992

Gene Structure 5773 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000032853

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1557 bp

ATGAGATTTCGGGGTTGGAGTGGTCTGAAAGGGGATGGGTGCCGTGGTTTTGGGGTTGTTCTAGGCATTGGAGGGAGATGGGTTTTGGGTTTAGAGGCCCAAACCCTTAAACCTCTGATTCCCAAACTCATTTGCGCCTTAACCCTTAAACCCTCACCGCCAAAGCCGTCGACCCAATCACCATCTTCCTACGACCTCAACGGCGCCGTTTCTTCTTCAACACCCATTGATTTGACAATGGCCTCCGACGAACTAGACGGCGAGGTGAAGACCTTCAAGAGCTTGGGACTCTGCGACCAATTGGTCGAGGCCGTTGCCAAGCTGGACTGGCAAAACCCTACCCCCATTCAAGTCGAAGCAATCCCTCATGCCCTAGAAGGAAAGGACTTGATCGGACTTGCCCAAACCGGGTCGGGTAAAACCGCAGCTTTTGCCCTTCCCATATTGCAAGCTCTCATTGATTCTCCTCATGCATTTTTTGCATGCATTCTCGCCCCAACTAGGGAGCTTGCAATTCAGATTTCTGAGCAGTTTGAAGCTTTGGGGTATGAGTTTGGTGCCAAGTCCGCAGTGCTTGTTGGAGGAGTGGATATGGTCCAACAGACTATTATGCTTGCAAAGAGGCCACACATTATTGTTGCTACACCTGGACGCCTCATGGATCATGTATCCAACACAAAAGGATTTTCTCTTCGCACGTTGAAGTATTTGGTTCTAGATGAGGCAGACAGATTGCTTAATGAGGACTTTGAAAAGACACTTGATGACATTCTGAAAGTCATCCCTCGCGAACGGAGAACATATTTGTTTTCTGCTACCATGACTAAAAAGGTCAAGAAGCTGCAAAGGGCTTGTCTACGGAACCCTGTGAAGATTGAAGTAGCATCTAAATATTCTACCGTTGATACACTAAAGCAGCAGTATCGTTTTCTGCCATCCCAGCATAAGGACTGTTATCTTGTATATATTCTGACCGAGAAGTGTCAATGTACAACTATGGTTTTTACTCGGACATGTGATGCAACACTCCTATTGGCTACGATGCTTCGAAATCTTGGCATAAGAGCCACCCCTATTAGTGGTCATATGAGCCAGTCAAAAAGACTTGGTGCCTTAAATATGTTTAAAGCTGGAGAGTGTAATGTACTTATCTGCACTGATGTGGCAAGTAGAGGATTGGATATTCCATCGGTGGATATGGTTATTAATTATGATATTCCCTCAAATTCTAAGGATTATATTCATCGAGTAGGAAGGACTGCTCGTGCTGGACGATCTGGGGTAGCAATCTCACTAGTGAATCAGTATGAGTTGGAGTGGTATATACAGATAGAAAAGCTTATCGGCAAAAAGTTACCTGAATTTCCTGCTCAGGAAGTGGAGGTGCTTCAATTTTTAGAACGTGTCACAGAGGCCAAAAGATTGGCCACCTCGAAATTAAAAGAAAAAGGAAGCAAGAAGAGGAAGGGAGGAGGAAATGAAGGCGAGGACGATGTTGATATTGGAAAGTATTTAGGTCAGAAGGATGTCAAGTCCAAAAAGTTTACAAAAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010467 GO:0010501 GO:0016070 GO:0016072 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022613 GO:0031974 GO:0031981 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

519

Amino Acids

57.21

Weight (kDa)

9.22

Isoelectric Point (pI)

35.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	114 - 280	5.5e-47	DEAD/DEAH box helicase
Helicase_C	PF00271	320 - 424	1.5e-29	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005279)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G60990
fragaria_vesca	FvH4_3g15190 FvH4_3g15190 FvH4_3g15190
malus_domestica	MD10G1206500.v1.1
prunus_persica	Prupe.4G135800_v2.0.a1
pyrus_communis	pycom10g17630
rosa_chinensis	RchiOBHm_Chr2g0164771 RchiOBHm_Chr5g0025631
rosa_laevigata	RLG00000021484 RLG00000021488 RLG00000031602 RLG00000032853
rosa_multiflora	Rmu_sc0002137.1_g000001
rosa_roxburghii	Rroxscaffold_1G00053540 Rroxscaffold_1G00054150 Rroxscaffold_2G00083670 Rroxscaffold_2G00086310
rosa_rugosa	Rorug02G0510100 Rorug02G0529200 Rorug05G0087300
rosa_samantha	Rh2AG578400 Rh2BG589500 Rh2CG560100 Rh2DG599900 Rh5AG179100 Rh5BG178200 Rh5DG177400
rosa_wichuraiana	Rw5G016260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	302
AccB1I	GGYRCC	4 cut(s)	39, 203, 557, 1109
AccI	GTMKAC	2 cut(s)	170, 856
AccII	CGCG	1 cut(s)	789
AciI	CCGC	3 cut(s)	158, 423, 567
AclWI	GGATC	1 cut(s)	669
AcoI	YGGCCR	1 cut(s)	1425
AcsI	RAATTY	2 cut(s)	1225, 1361
AcyI	GRCGYC	2 cut(s)	204, 652
AfaI	GTAC	2 cut(s)	991, 1146
AfiI	CCNNNNNNNGG	5 cut(s)	408, 501, 502, 946, 1079
AflIII	ACRYGT	2 cut(s)	1013, 1402
AgsI	TTSAA	8 cut(s)	219, 277, 350, 536, 703, 752, 878, 1391
AhlI	ACTAGT	1 cut(s)	1294
AjnI	CCWGG	1 cut(s)	646
AleI	CACNNNNGTG	1 cut(s)	1160
AluBI	AGCT	9 cut(s)	282, 322, 428, 452, 508, 539, 841, 1130, 1339
AluI	AGCT	9 cut(s)	282, 322, 428, 452, 508, 539, 841, 1130, 1339
AlwI	GGATC	1 cut(s)	669
AoxI	GGCC	6 cut(s)	96, 240, 309, 623, 1413, 1425
ApeKI	GCWGC	3 cut(s)	425, 841, 916
ApoI	RAATTY	2 cut(s)	1225, 1361
ArsI	GACNNNNNNTTYG	2 cut(s)	838, 870
AseI	ATTAAT	1 cut(s)	1206
Asp700I	GAANNNNTTC	1 cut(s)	272
AspLEI	GCGC	2 cut(s)	137, 206
AspS9I	GGNCC	2 cut(s)	97, 595
AsuC2I	CCSGG	1 cut(s)	409
AsuHPI	GGTGA	3 cut(s)	147, 171, 277
AsuII	TTCGAA	1 cut(s)	1048
AvaII	GGWCC	1 cut(s)	595
BalI	TGGCCA	1 cut(s)	1427
BanI	GGYRCC	4 cut(s)	39, 203, 557, 1109
BauI	CACGAG	1 cut(s)	1263
BbsI	GAAGAC	1 cut(s)	275
BbvI	GCAGC	3 cut(s)	437, 828, 928
BccI	CCATC	5 cut(s)	29, 72, 190, 943, 1195
BceAI	ACGGC	6 cut(s)	27, 151, 191, 217, 274, 296
BcgI	CGANNNNNNTGC	4 cut(s)	296, 330, 905, 939
BciT130I	CCWGG	1 cut(s)	648
BciVI	GTATCC	1 cut(s)	679
BcnI	CCSGG	1 cut(s)	409
BcuI	ACTAGT	1 cut(s)	1294
BfaI	CTAG	6 cut(s)	62, 254, 374, 501, 716, 1295
BfoI	RGCGCY	1 cut(s)	207
BfuI	GTATCC	1 cut(s)	679
BisI	GCNGC	3 cut(s)	426, 842, 917
BlsI	GCNGC	3 cut(s)	427, 843, 918
Bme1390I	CCNGG	2 cut(s)	409, 648
Bme18I	GGWCC	1 cut(s)	595
BmgT120I	GGNCC	2 cut(s)	97, 595
BmiI	GGNNCC	5 cut(s)	41, 205, 559, 863, 1111
BmrFI	CCNGG	2 cut(s)	409, 648
BmsI	GCATC	3 cut(s)	893, 1009, 1032
BpiI	GAAGAC	1 cut(s)	275
BpmI	CTGGAG	1 cut(s)	1152
Bpu10I	CCTNAGC	1 cut(s)	1371
Bpu14I	TTCGAA	1 cut(s)	1048
BpuMI	CCSGG	1 cut(s)	409
BsaHI	GRCGYC	2 cut(s)	204, 652
BsaJI	CCNNGG	1 cut(s)	43
Bsc4I	CCNNNNNNNGG	5 cut(s)	408, 501, 502, 946, 1079
Bse1I	ACTGG	2 cut(s)	332, 1093
BseBI	CCWGG	1 cut(s)	648
BseDI	CCNNGG	1 cut(s)	43
BseGI	GGATG	4 cut(s)	40, 780, 935, 1531
BseLI	CCNNNNNNNGG	5 cut(s)	408, 501, 502, 946, 1079
BseMII	CTCAG	2 cut(s)	516, 1385
BseNI	ACTGG	2 cut(s)	332, 1093
BseRI	GAGGAG	3 cut(s)	456, 597, 1485
BseXI	GCAGC	3 cut(s)	437, 828, 928
BseYI	CCCAGC	1 cut(s)	939
BsgI	GTGCAG	1 cut(s)	1138
Bsh1236I	CGCG	1 cut(s)	789
BshFI	GGCC	6 cut(s)	98, 242, 311, 625, 1415, 1427
BshNI	GGYRCC	4 cut(s)	39, 203, 557, 1109
BsiSI	CCGG	1 cut(s)	408
BslFI	GGGAC	1 cut(s)	300
BslI	CCNNNNNNNGG	5 cut(s)	408, 501, 502, 946, 1079
BsmFI	GGGAC	1 cut(s)	300
BsmI	GAATGC	1 cut(s)	486
BsnI	GGCC	6 cut(s)	98, 242, 311, 625, 1415, 1427
Bsp119I	TTCGAA	1 cut(s)	1048
Bsp1407I	TGTACA	1 cut(s)	989
Bsp143I	GATC	3 cut(s)	390, 661, 1274
Bsp68I	TCGCGA	1 cut(s)	789
BspACI	CCGC	3 cut(s)	158, 423, 567
BspANI	GGCC	6 cut(s)	98, 242, 311, 625, 1415, 1427
BspCNI	CTCAG	2 cut(s)	517, 1384
BspFNI	CGCG	1 cut(s)	789
BspLI	GGNNCC	5 cut(s)	41, 205, 559, 863, 1111
BspPI	GGATC	1 cut(s)	669
BspT104I	TTCGAA	1 cut(s)	1048
BspT107I	GGYRCC	4 cut(s)	39, 203, 557, 1109
BsrGI	TGTACA	1 cut(s)	989
BsrI	ACTGG	2 cut(s)	332, 1093
BssECI	CCNNGG	1 cut(s)	43
BssMI	GATC	3 cut(s)	390, 661, 1274
BssNI	GRCGYC	2 cut(s)	204, 652
BssSI	CACGAG	1 cut(s)	1263
Bst2BI	CACGAG	1 cut(s)	1263
Bst2UI	CCWGG	1 cut(s)	648
Bst4CI	ACNGT	2 cut(s)	901, 953
Bst6I	CTCTTC	2 cut(s)	696, 1454
BstACI	GRCGYC	2 cut(s)	204, 652
BstAPI	GCANNNNNTGC	2 cut(s)	431, 479
BstAUI	TGTACA	1 cut(s)	989
BstBI	TTCGAA	1 cut(s)	1048
BstC8I	GCNNGC	4 cut(s)	450, 484, 510, 615
BstDEI	CTNAG	3 cut(s)	525, 1230, 1371
BstDSI	CCRYGG	1 cut(s)	43
BstF5I	GGATG	4 cut(s)	40, 780, 935, 1531
BstFNI	CGCG	1 cut(s)	789
BstH2I	RGCGCY	1 cut(s)	207
BstHHI	GCGC	2 cut(s)	137, 206
BstKTI	GATC	3 cut(s)	393, 664, 1277
BstMBI	GATC	3 cut(s)	390, 661, 1274
BstMWI	GCNNNNNNNGC	5 cut(s)	328, 431, 479, 566, 940
BstNI	CCWGG	1 cut(s)	648
BstNSI	RCATGY	2 cut(s)	486, 1017
BstSCI	CCNGG	2 cut(s)	407, 646
BstUI	CGCG	1 cut(s)	789
BstV1I	GCAGC	3 cut(s)	437, 828, 928
BstV2I	GAAGAC	1 cut(s)	275
BstXI	CCANNNNNNTGG	1 cut(s)	1423
BsuI	GTATCC	1 cut(s)	679
BsuRI	GGCC	6 cut(s)	98, 242, 311, 625, 1415, 1427
BtgI	CCRYGG	1 cut(s)	43
BtsCI	GGATG	4 cut(s)	40, 780, 935, 1531
BtsI	GCAGTG	1 cut(s)	576
BtsIMutI	CAGTG	2 cut(s)	576, 1155
BtuMI	TCGCGA	1 cut(s)	789
Cac8I	GCNNGC	4 cut(s)	450, 484, 510, 615
CfoI	GCGC	2 cut(s)	137, 206
Cfr13I	GGNCC	2 cut(s)	97, 595
CseI	GACGC	1 cut(s)	660
Csp6I	GTAC	2 cut(s)	990, 1145
CviAII	CATG	7 cut(s)	368, 470, 483, 658, 665, 820, 1014
CviQI	GTAC	2 cut(s)	990, 1145
DdeI	CTNAG	3 cut(s)	525, 1230, 1371
DinI	GGCGCC	1 cut(s)	205
DpnI	GATC	3 cut(s)	392, 663, 1276
DpnII	GATC	3 cut(s)	390, 661, 1274
DraI	TTTAAA	1 cut(s)	1126
DrdI	GACNNNNNNGTC	1 cut(s)	302
DseDI	GACNNNNNNGTC	1 cut(s)	302
EaeI	YGGCCR	1 cut(s)	1425
Eam1104I	CTCTTC	2 cut(s)	696, 1454
EarI	CTCTTC	2 cut(s)	696, 1454
Eco47I	GGWCC	1 cut(s)	595
EcoRII	CCWGG	1 cut(s)	646
EcoT22I	ATGCAT	2 cut(s)	475, 488
EgeI	GGCGCC	1 cut(s)	205
EheI	GGCGCC	1 cut(s)	205
FaeI	CATG	7 cut(s)	371, 473, 486, 661, 668, 823, 1017
FaqI	GGGAC	1 cut(s)	300
FatI	CATG	7 cut(s)	367, 469, 482, 657, 664, 819, 1013
FauNDI	CATATG	1 cut(s)	1086
FblI	GTMKAC	2 cut(s)	170, 856
Fnu4HI	GCNGC	3 cut(s)	426, 842, 917
FokI	GGATG	4 cut(s)	47, 767, 922, 1538
Fsp4HI	GCNGC	3 cut(s)	426, 842, 917
FspBI	CTAG	6 cut(s)	62, 254, 374, 501, 716, 1295
GlaI	GCGC	2 cut(s)	136, 205
GluI	GCNGC	3 cut(s)	426, 842, 917
GsaI	CCCAGC	1 cut(s)	943
GsuI	CTGGAG	1 cut(s)	1152
HaeII	RGCGCY	1 cut(s)	207
HaeIII	GGCC	6 cut(s)	98, 242, 311, 625, 1415, 1427
HapII	CCGG	1 cut(s)	408
HgaI	GACGC	1 cut(s)	660
HhaI	GCGC	2 cut(s)	137, 206
Hin1I	GRCGYC	2 cut(s)	204, 652
Hin1II	CATG	7 cut(s)	371, 473, 486, 661, 668, 823, 1017
Hin6I	GCGC	2 cut(s)	135, 204
HinP1I	GCGC	2 cut(s)	135, 204
HincII	GTYRAC	1 cut(s)	171
HindII	GTYRAC	1 cut(s)	171
HindIII	AAGCTT	2 cut(s)	537, 1337
HinfI	GANTC	4 cut(s)	118, 288, 461, 1300
HpaII	CCGG	1 cut(s)	408
HphI	GGTGA	3 cut(s)	147, 171, 277
Hpy166II	GTNNAC	3 cut(s)	171, 857, 1545
Hpy188III	TCNNGA	5 cut(s)	277, 716, 788, 835, 1373
Hpy8I	GTNNAC	3 cut(s)	171, 857, 1545
Hpy99I	CGWCG	2 cut(s)	172, 251
HpyAV	CCTTC	7 cut(s)	283, 371, 446, 1248, 1459, 1475, 1516
HpyCH4III	ACNGT	2 cut(s)	901, 953
HpyCH4IV	ACGT	2 cut(s)	698, 1402
HpyCH4V	TGCA	9 cut(s)	448, 473, 482, 486, 512, 617, 844, 1022, 1155
HpyF10VI	GCNNNNNNNGC	5 cut(s)	328, 431, 479, 566, 940
HpyF3I	CTNAG	3 cut(s)	525, 1230, 1371
HpySE526I	ACGT	2 cut(s)	698, 1402
Hsp92I	GRCGYC	2 cut(s)	204, 652
Hsp92II	CATG	7 cut(s)	371, 473, 486, 661, 668, 823, 1017
HspAI	GCGC	2 cut(s)	135, 204
KasI	GGCGCC	1 cut(s)	203
Kzo9I	GATC	3 cut(s)	390, 661, 1274
LmnI	GCTCC	1 cut(s)	505
Lsp1109I	GCAGC	3 cut(s)	437, 828, 928
LweI	GCATC	3 cut(s)	893, 1009, 1032
MaeI	CTAG	6 cut(s)	62, 254, 374, 501, 716, 1295
MaeII	ACGT	2 cut(s)	698, 1402
MaeIII	GTNAC	2 cut(s)	1353, 1405
MalI	GATC	3 cut(s)	392, 663, 1276
MboI	GATC	3 cut(s)	390, 661, 1274
MboII	GAAGA	7 cut(s)	177, 204, 207, 280, 683, 883, 1471
MfeI	CAATTG	1 cut(s)	299
MlsI	TGGCCA	1 cut(s)	1427
MluCI	AATT	7 cut(s)	299, 513, 1207, 1225, 1361, 1391, 1436
MluNI	TGGCCA	1 cut(s)	1427
Mly113I	GGCGCC	1 cut(s)	204
MlyI	GAGTC	1 cut(s)	282
MmeI	TCCRAC	5 cut(s)	270, 559, 622, 696, 1293
Mox20I	TGGCCA	1 cut(s)	1427
Mph1103I	ATGCAT	2 cut(s)	475, 488
MroXI	GAANNNNTTC	1 cut(s)	272
MscI	TGGCCA	1 cut(s)	1427
MseI	TTAA	8 cut(s)	108, 140, 147, 738, 1115, 1125, 1206, 1439
MslI	CAYNNNNRTG	1 cut(s)	1160
Msp20I	TGGCCA	1 cut(s)	1427
MspI	CCGG	1 cut(s)	408
MspR9I	CCNGG	2 cut(s)	409, 648
MunI	CAATTG	1 cut(s)	299
Mva1269I	GAATGC	1 cut(s)	486
MvaI	CCWGG	1 cut(s)	648
MvnI	CGCG	1 cut(s)	789
MwoI	GCNNNNNNNGC	5 cut(s)	328, 431, 479, 566, 940
NarI	GGCGCC	1 cut(s)	204
NciI	CCSGG	1 cut(s)	409
NdeI	CATATG	1 cut(s)	1086
NdeII	GATC	3 cut(s)	390, 661, 1274
NlaIII	CATG	7 cut(s)	371, 473, 486, 661, 668, 823, 1017
NlaIV	GGNNCC	5 cut(s)	41, 205, 559, 863, 1111
NmuCI	GTSAC	1 cut(s)	1405
NruI	TCGCGA	1 cut(s)	789
NsiI	ATGCAT	2 cut(s)	475, 488
NspI	RCATGY	2 cut(s)	486, 1017
NspV	TTCGAA	1 cut(s)	1048
OliI	CACNNNNGTG	1 cut(s)	1160
PaeI	GCATGC	1 cut(s)	486
PciI	ACATGT	1 cut(s)	1013
PcsI	WCGNNNNNNNCGW	1 cut(s)	1270
PctI	GAATGC	1 cut(s)	486
PdmI	GAANNNNTTC	1 cut(s)	272
PfeI	GAWTC	3 cut(s)	118, 461, 1300
PkrI	GCNGC	3 cut(s)	427, 843, 918
PleI	GAGTC	1 cut(s)	282
PluTI	GGCGCC	1 cut(s)	207
PpsI	GAGTC	1 cut(s)	282
PscI	ACATGT	1 cut(s)	1013
PshBI	ATTAAT	1 cut(s)	1206
Psp6I	CCWGG	1 cut(s)	646
PspFI	CCCAGC	1 cut(s)	939
PspGI	CCWGG	1 cut(s)	646
PspN4I	GGNNCC	5 cut(s)	41, 205, 559, 863, 1111
PspPI	GGNCC	2 cut(s)	97, 595
RruI	TCGCGA	1 cut(s)	789
RsaI	GTAC	2 cut(s)	991, 1146
RsaNI	GTAC	2 cut(s)	990, 1145
RseI	CAYNNNNRTG	1 cut(s)	1160
SalI	GTCGAC	1 cut(s)	169
SaqAI	TTAA	8 cut(s)	108, 140, 147, 738, 1115, 1125, 1206, 1439
SatI	GCNGC	3 cut(s)	426, 842, 917
Sau3AI	GATC	3 cut(s)	390, 661, 1274
Sau96I	GGNCC	2 cut(s)	97, 595
SchI	GAGTC	1 cut(s)	282
ScrFI	CCNGG	2 cut(s)	409, 648
SfaNI	GCATC	3 cut(s)	893, 1009, 1032
SfoI	GGCGCC	1 cut(s)	205
SfuI	TTCGAA	1 cut(s)	1048
SinI	GGWCC	1 cut(s)	595
SmiMI	CAYNNNNRTG	1 cut(s)	1160
SpeI	ACTAGT	1 cut(s)	1294
SphI	GCATGC	1 cut(s)	486
Sse9I	AATT	7 cut(s)	299, 513, 1207, 1225, 1361, 1391, 1436
SsiI	CCGC	3 cut(s)	158, 423, 567
SspDI	GGCGCC	1 cut(s)	203
SspI	AATATT	1 cut(s)	893
SspMI	CTAG	6 cut(s)	62, 254, 374, 501, 716, 1295
StyD4I	CCNGG	2 cut(s)	407, 646
TaaI	ACNGT	2 cut(s)	901, 953
TaiI	ACGT	2 cut(s)	701, 1405
TaqI	TCGA	6 cut(s)	170, 306, 354, 1048, 1246, 1433
TaqII	GACCGA	1 cut(s)	989
TasI	AATT	7 cut(s)	299, 513, 1207, 1225, 1361, 1391, 1436
TatI	WGTACW	2 cut(s)	989, 1144
TfiI	GAWTC	3 cut(s)	118, 461, 1300
Tru1I	TTAA	8 cut(s)	108, 140, 147, 738, 1115, 1125, 1206, 1439
Tru9I	TTAA	8 cut(s)	108, 140, 147, 738, 1115, 1125, 1206, 1439
TscAI	CASTG	2 cut(s)	576, 1162
TseFI	GTSAC	1 cut(s)	1405
TseI	GCWGC	3 cut(s)	425, 841, 916
Tsp45I	GTSAC	1 cut(s)	1405
TspDTI	ATGAA	2 cut(s)	1232, 1494
TspGWI	ACGGA	2 cut(s)	808, 874
TspRI	CASTG	2 cut(s)	576, 1162
VpaK11BI	GGWCC	1 cut(s)	595
VspI	ATTAAT	1 cut(s)	1206
XapI	RAATTY	2 cut(s)	1225, 1361
XbaI	TCTAGA	1 cut(s)	715
XceI	RCATGY	2 cut(s)	486, 1017
XmiI	GTMKAC	2 cut(s)	170, 856
XmnI	GAANNNNTTC	1 cut(s)	272
XspI	CTAG	6 cut(s)	62, 254, 374, 501, 716, 1295
Zsp2I	ATGCAT	2 cut(s)	475, 488

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.