MD10G1224500.v1.1

BEST Arabidopsis thaliana protein match is

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0007028
Physical Location & Seq
Forward (+)
32215589 .. 32216882
1294 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1224500.v1.1.491

Sequence Viewer

Length: 963 bp
ATGGATTATCCGTTTTCGGGCGAAATGAGTGACTATTTTACTCTTGGAGACCTAGAAAAGCAAATTTCTGTAGATCCAATATCACTGAAAGAATCTTATAGAGAGGTGAGTGCTGCCCCAGTAGCGGTGGTGCCTGGTGTTGCTGCTCCAAAGCCGAATGCACTTGACCAACTACCGCCCCTGTTGCCGCCCTCAAAGCCCAAGTTCTTAAGCTTCAGCCTCCCAAACTCAGCAACCTCATCGCCGCGAGTGAAGAAGAAATGGAAAGAAGAAAGCCAAGCGTCTCCCCGGCTGGAGAATCTGAGCCACCTGCAGCAGGAAATTGAGTTCTGGCGGACCAAGTCATGTGGTCAACGAGCACTTGCCCCATCAGATGACTTTGATCTCTGGTTGTTCAAACCGAATGCCGCTGATAAAGTCACCACGTACCACAGCAGCCTCTCCAGAGCTAGGTCCAACAGAGGTGCTGATAAAAATGGAAAGAACAGGGACCCACATGATGACGGATTCACGTGTGGGGCCCTGTGCTTGTTCATTCCTGGCTTTGGTGGCAAGGCAAAGCCAGTGAGAGCAAGAAAGGTAGAGCCTGATGAATTGGGAAATGTCATATCCAGGACAGTTTCCTTGGAAAAGTTTGAATGTGGCTCTTGGGCTTCAGCTATGATAAATGAGCAGGAAGATGACACCACCGGTCACTACTTTGATCTGCCATTGGAGCTGATACGGAGCAGCGTCAACGAGGCAGAATCGCCAATTTCAACGTCTTTTGTGTTTGGCACGGATCGAAAGGGTGCCCTGAAGAACGCATCAACGAGAGCACAAGGCTGGAAATCGCACGACTCGTCACGCCACGTTCGGTTTTCTACATCATCAGATGCTACATCACCAACTGCTTGCGTTACGCCTCGCTTGCGCAAAGCAAGGGATGATTTCAAAGCTTTCTTAGAAGCACAGAGTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

321

Amino Acids

35.3

Weight (kDa)

8.19

Isoelectric Point (pI)

44.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 318
Acc16I TGCGCA 1 cut(s) 914
Acc36I ACCTGC 1 cut(s) 318
AccB1I GGYRCC 2 cut(s) 130, 791
AccII CGCG 1 cut(s) 247
AciI CCGC 6 cut(s) 125, 176, 188, 245, 334, 408
AclWI GGATC 2 cut(s) 68, 789
AcsI RAATTY 1 cut(s) 63
AcuI CTGAAG 3 cut(s) 199, 639, 818
AcvI CACGTG 1 cut(s) 513
AfaI GTAC 2 cut(s) 428, 958
AfiI CCNNNNNNNGG 5 cut(s) 17, 124, 316, 450, 545
AflII CTTAAG 1 cut(s) 208
AflIII ACRYGT 1 cut(s) 512
AgeI ACCGGT 1 cut(s) 689
AgsI TTSAA 4 cut(s) 397, 638, 759, 934
AjnI CCWGG 3 cut(s) 133, 538, 611
AloI GAACNNNNNNTCC 2 cut(s) 311, 343
AluBI AGCT 5 cut(s) 213, 449, 659, 718, 938
AluI AGCT 5 cut(s) 213, 449, 659, 718, 938
Alw21I GWGCWC 2 cut(s) 361, 820
Alw26I GTCTC 2 cut(s) 42, 288
AlwI GGATC 2 cut(s) 68, 789
AoxI GGCC 1 cut(s) 519
ApaI GGGCCC 1 cut(s) 523
ApeKI GCWGC 5 cut(s) 113, 143, 313, 435, 729
ApoI RAATTY 1 cut(s) 63
AsiGI ACCGGT 1 cut(s) 689
AspLEI GCGC 1 cut(s) 915
AspS9I GGNCC 5 cut(s) 336, 453, 490, 519, 520
AsuC2I CCSGG 1 cut(s) 289
AsuHPI GGTGA 3 cut(s) 118, 412, 876
AvaII GGWCC 3 cut(s) 336, 453, 490
BaeGI GKGCMC 2 cut(s) 523, 796
BanI GGYRCC 2 cut(s) 130, 791
BanII GRGCYC 1 cut(s) 523
BbrPI CACGTG 1 cut(s) 513
Bbv12I GWGCWC 2 cut(s) 361, 820
BbvI GCAGC 5 cut(s) 100, 130, 325, 447, 741
BccI CCATC 1 cut(s) 376
BcgI CGANNNNNNTGC 2 cut(s) 222, 256
BciT130I CCWGG 3 cut(s) 135, 540, 613
BcnI CCSGG 1 cut(s) 289
BcoDI GTCTC 2 cut(s) 42, 288
BfaI CTAG 2 cut(s) 53, 450
BfmI CTRYAG 2 cut(s) 69, 311
BfrI CTTAAG 1 cut(s) 208
BfuAI ACCTGC 1 cut(s) 318
BisI GCNGC 8 cut(s) 114, 144, 188, 245, 314, 408, 436, 730
BlsI GCNGC 8 cut(s) 115, 145, 189, 246, 315, 409, 437, 731
Bme1390I CCNGG 4 cut(s) 135, 289, 540, 613
Bme18I GGWCC 3 cut(s) 336, 453, 490
BmgT120I GGNCC 5 cut(s) 336, 453, 490, 519, 520
BmiI GGNNCC 6 cut(s) 132, 491, 492, 520, 521, 793
BmrFI CCNGG 4 cut(s) 135, 289, 540, 613
BmrI ACTGGG 1 cut(s) 113
BmsI GCATC 2 cut(s) 815, 865
BmuI ACTGGG 1 cut(s) 113
BpmI CTGGAG 2 cut(s) 314, 427
BpuMI CCSGG 1 cut(s) 289
BsaAI YACGTR 2 cut(s) 426, 513
BsaI GGTCTC 1 cut(s) 42
BsaJI CCNNGG 2 cut(s) 287, 624
BsaWI WCCGGW 1 cut(s) 689
Bsc4I CCNNNNNNNGG 5 cut(s) 17, 124, 316, 450, 545
Bse118I RCCGGY 1 cut(s) 689
Bse1I ACTGG 2 cut(s) 119, 563
BseBI CCWGG 3 cut(s) 135, 540, 613
BseDI CCNNGG 2 cut(s) 287, 624
BseGI GGATG 1 cut(s) 931
BseLI CCNNNNNNNGG 5 cut(s) 17, 124, 316, 450, 545
BseMII CTCAG 2 cut(s) 243, 293
BseNI ACTGG 2 cut(s) 119, 563
BseSI GKGCMC 2 cut(s) 523, 796
BseXI GCAGC 5 cut(s) 100, 130, 325, 447, 741
Bsh1236I CGCG 1 cut(s) 247
BshFI GGCC 1 cut(s) 521
BshNI GGYRCC 2 cut(s) 130, 791
BshTI ACCGGT 1 cut(s) 689
BsiHKAI GWGCWC 2 cut(s) 361, 820
BsiSI CCGG 2 cut(s) 289, 690
BslFI GGGAC 1 cut(s) 503
BslI CCNNNNNNNGG 5 cut(s) 17, 124, 316, 450, 545
BsmAI GTCTC 2 cut(s) 42, 288
BsmBI CGTCTC 1 cut(s) 288
BsmFI GGGAC 1 cut(s) 503
BsmI GAATGC 2 cut(s) 163, 409
BsnI GGCC 1 cut(s) 521
Bso31I GGTCTC 1 cut(s) 42
Bsp120I GGGCCC 1 cut(s) 519
Bsp1286I GDGCHC 4 cut(s) 361, 523, 796, 820
Bsp143I GATC 4 cut(s) 73, 382, 703, 781
BspACI CCGC 6 cut(s) 125, 176, 188, 245, 334, 408
BspANI GGCC 1 cut(s) 521
BspCNI CTCAG 2 cut(s) 242, 294
BspFNI CGCG 1 cut(s) 247
BspLI GGNNCC 6 cut(s) 132, 491, 492, 520, 521, 793
BspMAI CTGCAG 1 cut(s) 315
BspMI ACCTGC 1 cut(s) 318
BspPI GGATC 2 cut(s) 68, 789
BspT107I GGYRCC 2 cut(s) 130, 791
BspTI CTTAAG 1 cut(s) 208
BspTNI GGTCTC 1 cut(s) 42
BsrFI RCCGGY 1 cut(s) 689
BsrI ACTGG 2 cut(s) 119, 563
BssAI RCCGGY 1 cut(s) 689
BssECI CCNNGG 2 cut(s) 287, 624
BssMI GATC 4 cut(s) 73, 382, 703, 781
BssT1I CCWWGG 1 cut(s) 624
Bst2UI CCWGG 3 cut(s) 135, 540, 613
Bst4CI ACNGT 1 cut(s) 619
BstAFI CTTAAG 1 cut(s) 208
BstBAI YACGTR 2 cut(s) 426, 513
BstC8I GCNNGC 2 cut(s) 895, 911
BstDEI CTNAG 3 cut(s) 229, 302, 943
BstENI CCTNNNNNAGG 1 cut(s) 314
BstF5I GGATG 1 cut(s) 931
BstFNI CGCG 1 cut(s) 247
BstHHI GCGC 1 cut(s) 915
BstKTI GATC 4 cut(s) 76, 385, 706, 784
BstMAI GTCTC 2 cut(s) 42, 288
BstMBI GATC 4 cut(s) 73, 382, 703, 781
BstMWI GCNNNNNNNGC 6 cut(s) 122, 184, 196, 549, 715, 910
BstNI CCWGG 3 cut(s) 135, 540, 613
BstSCI CCNGG 4 cut(s) 133, 287, 538, 611
BstSFI CTRYAG 2 cut(s) 69, 311
BstSLI GKGCMC 2 cut(s) 523, 796
BstUI CGCG 1 cut(s) 247
BstV1I GCAGC 5 cut(s) 100, 130, 325, 447, 741
BstX2I RGATCY 1 cut(s) 73
BstYI RGATCY 1 cut(s) 73
BsuRI GGCC 1 cut(s) 521
BtgZI GCGATG 1 cut(s) 225
BtsCI GGATG 1 cut(s) 931
BtsIMutI CAGTG 2 cut(s) 83, 570
BveI ACCTGC 1 cut(s) 318
Cac8I GCNNGC 2 cut(s) 895, 911
CfoI GCGC 1 cut(s) 915
Cfr10I RCCGGY 1 cut(s) 689
Cfr13I GGNCC 5 cut(s) 336, 453, 490, 519, 520
CseI GACGC 2 cut(s) 270, 721
Csp6I GTAC 2 cut(s) 427, 957
CspAI ACCGGT 1 cut(s) 689
CspCI CAANNNNNGTGG 2 cut(s) 328, 363
CviAII CATG 2 cut(s) 345, 497
CviQI GTAC 2 cut(s) 427, 957
DdeI CTNAG 3 cut(s) 229, 302, 943
DpnI GATC 4 cut(s) 75, 384, 705, 783
DpnII GATC 4 cut(s) 73, 382, 703, 781
EciI GGCGGA 1 cut(s) 349
Eco130I CCWWGG 1 cut(s) 624
Eco24I GRGCYC 1 cut(s) 523
Eco31I GGTCTC 1 cut(s) 42
Eco47I GGWCC 3 cut(s) 336, 453, 490
Eco57I CTGAAG 3 cut(s) 199, 639, 818
Eco72I CACGTG 1 cut(s) 513
EcoNI CCTNNNNNAGG 1 cut(s) 314
EcoO109I RGGNCCY 3 cut(s) 490, 519, 520
EcoRII CCWGG 3 cut(s) 133, 538, 611
EcoT14I CCWWGG 1 cut(s) 624
EcoT38I GRGCYC 1 cut(s) 523
ErhI CCWWGG 1 cut(s) 624
Esp3I CGTCTC 1 cut(s) 288
FaeI CATG 2 cut(s) 348, 500
FaiI YATR 6 cut(s) 99, 346, 498, 608, 662, 961
FaqI GGGAC 1 cut(s) 503
FatI CATG 2 cut(s) 344, 496
Fnu4HI GCNGC 8 cut(s) 114, 144, 188, 245, 314, 408, 436, 730
FokI GGATG 1 cut(s) 938
FriOI GRGCYC 1 cut(s) 523
Fsp4HI GCNGC 8 cut(s) 114, 144, 188, 245, 314, 408, 436, 730
FspBI CTAG 2 cut(s) 53, 450
FspI TGCGCA 1 cut(s) 914
GlaI GCGC 1 cut(s) 914
GluI GCNGC 8 cut(s) 114, 144, 188, 245, 314, 408, 436, 730
GsuI CTGGAG 2 cut(s) 314, 427
HaeIII GGCC 1 cut(s) 521
HapII CCGG 2 cut(s) 289, 690
HgaI GACGC 2 cut(s) 270, 721
HhaI GCGC 1 cut(s) 915
Hin1II CATG 2 cut(s) 348, 500
Hin6I GCGC 1 cut(s) 913
HinP1I GCGC 1 cut(s) 913
HincII GTYRAC 2 cut(s) 353, 736
HindII GTYRAC 2 cut(s) 353, 736
HindIII AAGCTT 2 cut(s) 211, 936
HinfI GANTC 5 cut(s) 92, 298, 507, 746, 839
HpaII CCGG 2 cut(s) 289, 690
HphI GGTGA 3 cut(s) 118, 412, 876
Hpy166II GTNNAC 2 cut(s) 353, 736
Hpy188I TCNGA 3 cut(s) 303, 373, 874
Hpy188III TCNNGA 1 cut(s) 444
Hpy8I GTNNAC 2 cut(s) 353, 736
HpyCH4III ACNGT 1 cut(s) 619
HpyCH4IV ACGT 4 cut(s) 425, 512, 761, 852
HpyCH4V TGCA 2 cut(s) 161, 313
HpyF10VI GCNNNNNNNGC 6 cut(s) 122, 184, 196, 549, 715, 910
HpyF3I CTNAG 3 cut(s) 229, 302, 943
HpySE526I ACGT 4 cut(s) 425, 512, 761, 852
Hsp92II CATG 2 cut(s) 348, 500
HspAI GCGC 1 cut(s) 913
KflI GGGWCCC 1 cut(s) 490
Kzo9I GATC 4 cut(s) 73, 382, 703, 781
LmnI GCTCC 3 cut(s) 151, 715, 726
Lsp1109I GCAGC 5 cut(s) 100, 130, 325, 447, 741
LweI GCATC 2 cut(s) 815, 865
MaeI CTAG 2 cut(s) 53, 450
MaeII ACGT 4 cut(s) 425, 512, 761, 852
MaeIII GTNAC 5 cut(s) 29, 418, 692, 843, 898
MalI GATC 4 cut(s) 75, 384, 705, 783
MboI GATC 4 cut(s) 73, 382, 703, 781
MboII GAAGA 5 cut(s) 265, 268, 281, 689, 811
MflI RGATCY 1 cut(s) 73
MhlI GDGCHC 4 cut(s) 361, 523, 796, 820
MluCI AATT 4 cut(s) 63, 321, 593, 753
MlyI GAGTC 1 cut(s) 833
MmeI TCCRAC 1 cut(s) 480
MnlI CCTC 8 cut(s) 97, 202, 230, 247, 449, 455, 733, 915
MseI TTAA 1 cut(s) 209
MspA1I CMGCKG 1 cut(s) 410
MspCI CTTAAG 1 cut(s) 208
MspI CCGG 2 cut(s) 289, 690
MspR9I CCNGG 4 cut(s) 135, 289, 540, 613
Mva1269I GAATGC 2 cut(s) 163, 409
MvaI CCWGG 3 cut(s) 135, 540, 613
MvnI CGCG 1 cut(s) 247
MwoI GCNNNNNNNGC 6 cut(s) 122, 184, 196, 549, 715, 910
NciI CCSGG 1 cut(s) 289
NdeII GATC 4 cut(s) 73, 382, 703, 781
NlaIII CATG 2 cut(s) 348, 500
NlaIV GGNNCC 6 cut(s) 132, 491, 492, 520, 521, 793
NmuCI GTSAC 4 cut(s) 29, 418, 692, 843
NsbI TGCGCA 1 cut(s) 914
PaqCI CACCTGC 1 cut(s) 318
PcsI WCGNNNNNNNCGW 1 cut(s) 839
PctI GAATGC 2 cut(s) 163, 409
PfeI GAWTC 4 cut(s) 92, 298, 507, 746
PflFI GACNNNGTC 1 cut(s) 340
PfoI TCCNGGA 1 cut(s) 611
PinAI ACCGGT 1 cut(s) 689
PkrI GCNGC 8 cut(s) 115, 145, 189, 246, 315, 409, 437, 731
PleI GAGTC 1 cut(s) 833
PmaCI CACGTG 1 cut(s) 513
PmlI CACGTG 1 cut(s) 513
PpsI GAGTC 1 cut(s) 833
Ppu21I YACGTR 2 cut(s) 426, 513
PpuMI RGGWCCY 1 cut(s) 490
Psp5II RGGWCCY 1 cut(s) 490
Psp6I CCWGG 3 cut(s) 133, 538, 611
PspCI CACGTG 1 cut(s) 513
PspGI CCWGG 3 cut(s) 133, 538, 611
PspN4I GGNNCC 6 cut(s) 132, 491, 492, 520, 521, 793
PspOMI GGGCCC 1 cut(s) 519
PspPI GGNCC 5 cut(s) 336, 453, 490, 519, 520
PspPPI RGGWCCY 1 cut(s) 490
PstI CTGCAG 1 cut(s) 315
PsuI RGATCY 1 cut(s) 73
PsyI GACNNNGTC 1 cut(s) 340
RsaI GTAC 2 cut(s) 428, 958
RsaNI GTAC 2 cut(s) 427, 957
SaqAI TTAA 1 cut(s) 209
SatI GCNGC 8 cut(s) 114, 144, 188, 245, 314, 408, 436, 730
Sau3AI GATC 4 cut(s) 73, 382, 703, 781
Sau96I GGNCC 5 cut(s) 336, 453, 490, 519, 520
SchI GAGTC 1 cut(s) 833
ScrFI CCNGG 4 cut(s) 135, 289, 540, 613
SduI GDGCHC 4 cut(s) 361, 523, 796, 820
SfaNI GCATC 2 cut(s) 815, 865
SfcI CTRYAG 2 cut(s) 69, 311
SinI GGWCC 3 cut(s) 336, 453, 490
SmlI CTYRAG 1 cut(s) 208
SmoI CTYRAG 1 cut(s) 208
Sse9I AATT 4 cut(s) 63, 321, 593, 753
SsiI CCGC 6 cut(s) 125, 176, 188, 245, 334, 408
SspMI CTAG 2 cut(s) 53, 450
StyD4I CCNGG 4 cut(s) 133, 287, 538, 611
StyI CCWWGG 1 cut(s) 624
TaaI ACNGT 1 cut(s) 619
TaiI ACGT 4 cut(s) 428, 515, 764, 855
TaqI TCGA 1 cut(s) 784
TasI AATT 4 cut(s) 63, 321, 593, 753
TatI WGTACW 1 cut(s) 956
TauI GCSGC 3 cut(s) 190, 247, 410
TfiI GAWTC 4 cut(s) 92, 298, 507, 746
Tru1I TTAA 1 cut(s) 209
Tru9I TTAA 1 cut(s) 209
TscAI CASTG 2 cut(s) 90, 570
TseFI GTSAC 4 cut(s) 29, 418, 692, 843
TseI GCWGC 5 cut(s) 113, 143, 313, 435, 729
Tsp45I GTSAC 4 cut(s) 29, 418, 692, 843
TspDTI ATGAA 2 cut(s) 523, 606
TspGWI ACGGA 3 cut(s) 519, 739, 794
TspRI CASTG 2 cut(s) 90, 570
Tth111I GACNNNGTC 1 cut(s) 340
Vha464I CTTAAG 1 cut(s) 208
VpaK11BI GGWCC 3 cut(s) 336, 453, 490
XagI CCTNNNNNAGG 1 cut(s) 314
XapI RAATTY 1 cut(s) 63
XspI CTAG 2 cut(s) 53, 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.