Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0003571

Physical Location & Seq

Genomic Coordinates Forward (+)

38756672 .. 38759532

Gene Structure 2861 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1300300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 726 bp

ATGTCCCTGTTCTGGATTGTTGTTCATCAGCTTGCAGAGATCGAAGCAATGGCCGCGTCGAAGAAAGTCATAACGAGGGAAGAGTGGGAGAAGAAGCTCAAGGATGTAAAAATTAGGAAAGAAGACATGAATAAATTGGTGATGAACTTCCTTGTCACTGAGGGTTATGTGGATGCTGCTGAGAAATTCCGTAAGGAATCTGGAACCGAACATATTGATCTTGCAACAATAACGGAACGCATGGCTGTCAAGAAGGCAGTACAATGCGGTAATGTGGAGGATGCGATTGAGAAAGTGAATGACTTAAACCCTGAGATTCTGGATACAAATCCTCAATTATTTTTCCATCTCCAACAGCAAAGGTTGATAGAACTAATTCGGAATGGAAAAGTCGAAGAGGCTCTTGAGTTTGCTCAGGAAGAGCTAGCACCAAGGGGAGAAGAAAATCAAAGCTTTTTAGAAGAGTTGGAGAGGACTGTTGCACTGCTAGCTTTTGAAGATGTGAACAACTGTCCCGTCGGAGAGCTTCTCGACATATCACAGCGCCTAAAGACAGCAAGTGAGGTGAATGCAGCCATCCTTACCAGCCAGAGTCATGAAAAAGATCCGAAGCTTCCAAGCTTATTGAAGATGCTGTTATGGGCTCAGAACCAGCTTGATGAAAAAGCCGCTTATCCTCGGATAAACGATCTTTCCACTGCCAAGCTAGAAGACCCTACCGTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CLTH LisH

Protein Analysis

242

Amino Acids

27.48

Weight (kDa)

4.87

Isoelectric Point (pI)

41.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LisH	PF08513	41 - 67	3e-09	LisH
CTLH	PF10607	76 - 219	7.4e-39	CTLH/CRA C-terminal to LisH motif domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003571)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G61150 AT1G61150 AT1G61150 AT1G61150 AT1G61150 AT1G61150 AT1G61150 AT1G61150 AT1G61150
fragaria_vesca	FvH4_3g04570 FvH4_3g04570 FvH4_3g04570 FvH4_6g14210
malus_domestica	MD05G1322300.v1.1 MD10G1300300.v1.1
prunus_persica	Prupe.4G040500_v2.0.a1 Prupe.4G040500_v2.0.a1 Prupe.4G040500_v2.0.a1 Prupe.4G040500_v2.0.a1 Prupe.4G040500_v2.0.a1
pyrus_communis	pycom05g29660 pycom10g25300
rosa_chinensis	RchiOBHm_Chr5g0007491
rosa_laevigata	RLG00000031461
rosa_multiflora	Rmu_co8051752.1_g000001 Rmu_ssc0000182.1_g000006
rosa_roxburghii	Rroxscaffold_1G00068900
rosa_rugosa	Rorug04G0427200 Rorug04G0427300 Rorug04G0427400 Rorug04G0427600
rosa_samantha	Rh5AG055600 Rh5CG063000 Rh5DG052300
rosa_wichuraiana	Rw5G005450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	719
AccII	CGCG	1 cut(s)	56
AciI	CCGC	3 cut(s)	54, 267, 669
AclWI	GGATC	1 cut(s)	599
AcoI	YGGCCR	1 cut(s)	51
AcsI	RAATTY	1 cut(s)	185
AfaI	GTAC	1 cut(s)	261
AfiI	CCNNNNNNNGG	1 cut(s)	12
AgsI	TTSAA	2 cut(s)	497, 628
AlwI	GGATC	1 cut(s)	599
AoxI	GGCC	1 cut(s)	51
ApeKI	GCWGC	2 cut(s)	176, 572
ApoI	RAATTY	1 cut(s)	185
Asp700I	GAANNNNTTC	1 cut(s)	375
AspLEI	GCGC	1 cut(s)	546
AsuHPI	GGTGA	2 cut(s)	151, 577
AsuNHI	GCTAGC	2 cut(s)	424, 487
BanII	GRGCYC	1 cut(s)	646
BbsI	GAAGAC	2 cut(s)	129, 717
BbvI	GCAGC	2 cut(s)	163, 584
BccI	CCATC	2 cut(s)	354, 584
BciVI	GTATCC	1 cut(s)	316
BfaI	CTAG	4 cut(s)	425, 488, 707, 724
BfoI	RGCGCY	1 cut(s)	547
BfuI	GTATCC	1 cut(s)	316
BisI	GCNGC	4 cut(s)	54, 177, 573, 669
BlsI	GCNGC	4 cut(s)	55, 178, 574, 670
BmiI	GGNNCC	1 cut(s)	205
BmsI	GCATC	3 cut(s)	163, 271, 621
BmtI	GCTAGC	2 cut(s)	428, 491
BpiI	GAAGAC	2 cut(s)	129, 717
BplI	GAGNNNNNCTC	2 cut(s)	513, 545
Bpu10I	CCTNAGC	1 cut(s)	414
BpuEI	CTTGAG	2 cut(s)	83, 425
BsaBI	GATNNNNATC	1 cut(s)	327
BsaJI	CCNNGG	2 cut(s)	431, 677
Bsc4I	CCNNNNNNNGG	1 cut(s)	12
Bse3DI	GCAATG	1 cut(s)	54
Bse8I	GATNNNNATC	1 cut(s)	327
BseDI	CCNNGG	2 cut(s)	431, 677
BseGI	GGATG	4 cut(s)	109, 178, 286, 576
BseJI	GATNNNNATC	1 cut(s)	327
BseLI	CCNNNNNNNGG	1 cut(s)	12
BseMI	GCAATG	1 cut(s)	54
BseMII	CTCAG	5 cut(s)	150, 171, 303, 428, 659
BseXI	GCAGC	2 cut(s)	163, 584
Bsh1236I	CGCG	1 cut(s)	56
BshFI	GGCC	1 cut(s)	53
BslFI	GGGAC	1 cut(s)	498
BslI	CCNNNNNNNGG	1 cut(s)	12
BsmFI	GGGAC	1 cut(s)	498
BsmI	GAATGC	1 cut(s)	574
BsnI	GGCC	1 cut(s)	53
Bsp1286I	GDGCHC	1 cut(s)	646
Bsp143I	GATC	4 cut(s)	39, 217, 604, 688
BspACI	CCGC	3 cut(s)	54, 267, 669
BspANI	GGCC	1 cut(s)	53
BspCNI	CTCAG	5 cut(s)	151, 172, 304, 427, 658
BspFNI	CGCG	1 cut(s)	56
BspHI	TCATGA	1 cut(s)	595
BspLI	GGNNCC	1 cut(s)	205
BspOI	GCTAGC	2 cut(s)	428, 491
BspPI	GGATC	1 cut(s)	599
BspQI	GCTCTTC	1 cut(s)	414
BsrDI	GCAATG	1 cut(s)	54
BssECI	CCNNGG	2 cut(s)	431, 677
BssMI	GATC	4 cut(s)	39, 217, 604, 688
BssT1I	CCWWGG	1 cut(s)	431
Bst4CI	ACNGT	3 cut(s)	478, 512, 721
Bst6I	CTCTTC	4 cut(s)	75, 390, 414, 456
BstC8I	GCNNGC	3 cut(s)	33, 426, 489
BstDEI	CTNAG	5 cut(s)	159, 180, 312, 414, 645
BstF5I	GGATG	4 cut(s)	109, 178, 286, 576
BstFNI	CGCG	1 cut(s)	56
BstH2I	RGCGCY	1 cut(s)	547
BstHHI	GCGC	1 cut(s)	546
BstKTI	GATC	4 cut(s)	42, 220, 607, 691
BstMBI	GATC	4 cut(s)	39, 217, 604, 688
BstMWI	GCNNNNNNNGC	2 cut(s)	53, 488
BstUI	CGCG	1 cut(s)	56
BstV1I	GCAGC	2 cut(s)	163, 584
BstV2I	GAAGAC	2 cut(s)	129, 717
BstX2I	RGATCY	1 cut(s)	604
BstYI	RGATCY	1 cut(s)	604
BsuI	GTATCC	1 cut(s)	316
BsuRI	GGCC	1 cut(s)	53
BtsCI	GGATG	4 cut(s)	109, 178, 286, 576
BtsI	GCAGTG	2 cut(s)	482, 696
BtsIMutI	CAGTG	3 cut(s)	156, 482, 696
Cac8I	GCNNGC	3 cut(s)	33, 426, 489
CciI	TCATGA	1 cut(s)	595
CfoI	GCGC	1 cut(s)	546
CseI	GACGC	1 cut(s)	45
Csp6I	GTAC	1 cut(s)	260
CviAII	CATG	3 cut(s)	127, 241, 596
CviQI	GTAC	1 cut(s)	260
DdeI	CTNAG	5 cut(s)	159, 180, 312, 414, 645
DpnI	GATC	4 cut(s)	41, 219, 606, 690
DpnII	GATC	4 cut(s)	39, 217, 604, 688
DrdI	GACNNNNNNGTC	1 cut(s)	719
DseDI	GACNNNNNNGTC	1 cut(s)	719
EaeI	YGGCCR	1 cut(s)	51
Eam1104I	CTCTTC	4 cut(s)	75, 390, 414, 456
EarI	CTCTTC	4 cut(s)	75, 390, 414, 456
Eco130I	CCWWGG	1 cut(s)	431
Eco24I	GRGCYC	1 cut(s)	646
EcoT14I	CCWWGG	1 cut(s)	431
EcoT38I	GRGCYC	1 cut(s)	646
ErhI	CCWWGG	1 cut(s)	431
FaeI	CATG	3 cut(s)	130, 244, 599
FaiI	YATR	8 cut(s)	71, 128, 168, 213, 242, 536, 597, 640
FalI	AAGNNNNNCTT	2 cut(s)	387, 419
FaqI	GGGAC	1 cut(s)	498
FatI	CATG	3 cut(s)	126, 240, 595
Fnu4HI	GCNGC	4 cut(s)	54, 177, 573, 669
FokI	GGATG	4 cut(s)	116, 185, 293, 563
FriOI	GRGCYC	1 cut(s)	646
Fsp4HI	GCNGC	4 cut(s)	54, 177, 573, 669
FspBI	CTAG	4 cut(s)	425, 488, 707, 724
GlaI	GCGC	1 cut(s)	545
GluI	GCNGC	4 cut(s)	54, 177, 573, 669
HaeII	RGCGCY	1 cut(s)	547
HaeIII	GGCC	1 cut(s)	53
HgaI	GACGC	1 cut(s)	45
HhaI	GCGC	1 cut(s)	546
Hin1II	CATG	3 cut(s)	130, 244, 599
Hin6I	GCGC	1 cut(s)	544
HinP1I	GCGC	1 cut(s)	544
HindIII	AAGCTT	3 cut(s)	451, 611, 619
HinfI	GANTC	3 cut(s)	197, 316, 592
HphI	GGTGA	2 cut(s)	151, 577
Hpy166II	GTNNAC	1 cut(s)	505
Hpy188I	TCNGA	5 cut(s)	381, 521, 609, 648, 681
Hpy188III	TCNNGA	8 cut(s)	13, 201, 250, 320, 404, 416, 530, 596
Hpy8I	GTNNAC	1 cut(s)	505
Hpy99I	CGWCG	2 cut(s)	61, 521
HpyAV	CCTTC	1 cut(s)	247
HpyCH4III	ACNGT	3 cut(s)	478, 512, 721
HpyCH4V	TGCA	4 cut(s)	35, 224, 482, 572
HpyF10VI	GCNNNNNNNGC	2 cut(s)	53, 488
HpyF3I	CTNAG	5 cut(s)	159, 180, 312, 414, 645
Hsp92II	CATG	3 cut(s)	130, 244, 599
HspAI	GCGC	1 cut(s)	544
Kzo9I	GATC	4 cut(s)	39, 217, 604, 688
LguI	GCTCTTC	1 cut(s)	414
LpnPI	CCDG	8 cut(s)	20, 186, 305, 324, 401, 598, 602, 665
Lsp1109I	GCAGC	2 cut(s)	163, 584
LweI	GCATC	3 cut(s)	163, 271, 621
MaeI	CTAG	4 cut(s)	425, 488, 707, 724
MaeIII	GTNAC	1 cut(s)	154
MalI	GATC	4 cut(s)	41, 219, 606, 690
MboI	GATC	4 cut(s)	39, 217, 604, 688
MflI	RGATCY	1 cut(s)	604
MhlI	GDGCHC	1 cut(s)	646
MluCI	AATT	5 cut(s)	111, 134, 185, 335, 375
MlyI	GAGTC	1 cut(s)	601
MmeI	TCCRAC	3 cut(s)	376, 447, 499
MnlI	CCTC	8 cut(s)	69, 154, 271, 342, 391, 465, 556, 687
MroXI	GAANNNNTTC	1 cut(s)	375
MseI	TTAA	1 cut(s)	305
Mva1269I	GAATGC	1 cut(s)	574
MvnI	CGCG	1 cut(s)	56
MwoI	GCNNNNNNNGC	2 cut(s)	53, 488
NdeII	GATC	4 cut(s)	39, 217, 604, 688
NheI	GCTAGC	2 cut(s)	424, 487
NlaIII	CATG	3 cut(s)	130, 244, 599
NlaIV	GGNNCC	1 cut(s)	205
NmuCI	GTSAC	1 cut(s)	154
PagI	TCATGA	1 cut(s)	595
PciSI	GCTCTTC	1 cut(s)	414
PctI	GAATGC	1 cut(s)	574
PdmI	GAANNNNTTC	1 cut(s)	375
PfeI	GAWTC	2 cut(s)	197, 316
PkrI	GCNGC	4 cut(s)	55, 178, 574, 670
PleI	GAGTC	1 cut(s)	600
PpsI	GAGTC	1 cut(s)	600
PspN4I	GGNNCC	1 cut(s)	205
PsuI	RGATCY	1 cut(s)	604
RsaI	GTAC	1 cut(s)	261
RsaNI	GTAC	1 cut(s)	260
SapI	GCTCTTC	1 cut(s)	414
SaqAI	TTAA	1 cut(s)	305
SatI	GCNGC	4 cut(s)	54, 177, 573, 669
Sau3AI	GATC	4 cut(s)	39, 217, 604, 688
SchI	GAGTC	1 cut(s)	601
SduI	GDGCHC	1 cut(s)	646
SfaNI	GCATC	3 cut(s)	163, 271, 621
SmlI	CTYRAG	2 cut(s)	98, 404
SmoI	CTYRAG	2 cut(s)	98, 404
Sse9I	AATT	5 cut(s)	111, 134, 185, 335, 375
SsiI	CCGC	3 cut(s)	54, 267, 669
SspMI	CTAG	4 cut(s)	425, 488, 707, 724
StyI	CCWWGG	1 cut(s)	431
TaaI	ACNGT	3 cut(s)	478, 512, 721
TaqI	TCGA	4 cut(s)	42, 59, 393, 531
TasI	AATT	5 cut(s)	111, 134, 185, 335, 375
TatI	WGTACW	1 cut(s)	259
TauI	GCSGC	2 cut(s)	56, 671
TfiI	GAWTC	2 cut(s)	197, 316
Tru1I	TTAA	1 cut(s)	305
Tru9I	TTAA	1 cut(s)	305
TscAI	CASTG	3 cut(s)	163, 489, 703
TseFI	GTSAC	1 cut(s)	154
TseI	GCWGC	2 cut(s)	176, 572
Tsp45I	GTSAC	1 cut(s)	154
TspDTI	ATGAA	5 cut(s)	14, 143, 158, 612, 675
TspGWI	ACGGA	2 cut(s)	179, 248
TspRI	CASTG	3 cut(s)	163, 489, 703
XapI	RAATTY	1 cut(s)	185
XmnI	GAANNNNTTC	1 cut(s)	375
XspI	CTAG	4 cut(s)	425, 488, 707, 724

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.