Rorug04G0427600

CT11-RanBPM

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003571
Physical Location & Seq
Reverse (-)
58585250 .. 58585685
436 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0427600.1

Sequence Viewer

Length: 309 bp
ATGGCGGACTTGAGTCAAGCACCGAGTAAACCATACATCACAAAGGTTCGTGAGCTCCTCCCAATGGACTCTGGTTTCACTCTTACCGTAAAAGTAGTTGGTCGTACTAAGATGGTATTGCACAATGGACGCCAAATTGCTCAATGCTTGGTTGGCGATCAAACTAGAATGATAATCTTCAGATCTAGAAACGATCGAGCGATCTGTGGACTTGATGAAAGAGGGTTCGACTCTAACCCTGTGAAATGCCGAAATTTACATGTTCAAAGGAACAATGAGGCTTGCTGTCGGCCAAAGTGGCAGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLTH LisH

Protein Analysis

102

Amino Acids

11.63

Weight (kDa)

9.63

Isoelectric Point (pI)

39.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 19 - 66 2.5e-06 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 5
AcoI YGGCCR 1 cut(s) 290
AcsI RAATTY 1 cut(s) 253
AcuI CTGAAG 1 cut(s) 163
AcyI GRCGYC 1 cut(s) 130
AfaI GTAC 1 cut(s) 106
AfiI CCNNNNNNNGG 1 cut(s) 64
AflIII ACRYGT 1 cut(s) 259
AgsI TTSAA 1 cut(s) 266
AluBI AGCT 1 cut(s) 55
AluI AGCT 1 cut(s) 55
Alw21I GWGCWC 1 cut(s) 57
AoxI GGCC 1 cut(s) 290
ApoI RAATTY 1 cut(s) 253
BanII GRGCYC 1 cut(s) 57
Bbv12I GWGCWC 1 cut(s) 57
BccI CCATC 1 cut(s) 106
BfaI CTAG 2 cut(s) 165, 186
BglI GCCNNNNNGGC 1 cut(s) 298
BglII AGATCT 1 cut(s) 182
BoxI GACNNNNGTC 1 cut(s) 12
BpuEI CTTGAG 1 cut(s) 31
BsaHI GRCGYC 1 cut(s) 130
Bsc4I CCNNNNNNNGG 1 cut(s) 64
BseLI CCNNNNNNNGG 1 cut(s) 64
BseRI GAGGAG 1 cut(s) 47
Bsh1285I CGRYCG 1 cut(s) 196
BshFI GGCC 1 cut(s) 292
BsiEI CGRYCG 1 cut(s) 196
BsiHKAI GWGCWC 1 cut(s) 57
BslI CCNNNNNNNGG 1 cut(s) 64
BsnI GGCC 1 cut(s) 292
Bsp1286I GDGCHC 1 cut(s) 57
Bsp143I GATC 4 cut(s) 157, 182, 193, 201
BspACI CCGC 1 cut(s) 5
BspANI GGCC 1 cut(s) 292
BssMI GATC 4 cut(s) 157, 182, 193, 201
BssNI GRCGYC 1 cut(s) 130
Bst4CI ACNGT 1 cut(s) 88
BstACI GRCGYC 1 cut(s) 130
BstC8I GCNNGC 1 cut(s) 283
BstDEI CTNAG 1 cut(s) 108
BstKTI GATC 4 cut(s) 160, 185, 196, 204
BstMBI GATC 4 cut(s) 157, 182, 193, 201
BstMCI CGRYCG 1 cut(s) 196
BstMWI GCNNNNNNNGC 2 cut(s) 153, 298
BstNSI RCATGY 1 cut(s) 263
BstPAI GACNNNNGTC 1 cut(s) 12
BstX2I RGATCY 1 cut(s) 182
BstYI RGATCY 1 cut(s) 182
BsuRI GGCC 1 cut(s) 292
BtsI GCAGTG 1 cut(s) 308
BtsIMutI CAGTG 1 cut(s) 308
Cac8I GCNNGC 1 cut(s) 283
CseI GACGC 1 cut(s) 138
Csp6I GTAC 1 cut(s) 105
CviAII CATG 1 cut(s) 260
CviJI RGCY 3 cut(s) 55, 281, 292
CviKI_1 RGCY 3 cut(s) 55, 281, 292
CviQI GTAC 1 cut(s) 105
DdeI CTNAG 1 cut(s) 108
DpnI GATC 4 cut(s) 159, 184, 195, 203
DpnII GATC 4 cut(s) 157, 182, 193, 201
EaeI YGGCCR 1 cut(s) 290
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 55
Eco24I GRGCYC 1 cut(s) 57
Eco53kI GAGCTC 1 cut(s) 55
Eco57I CTGAAG 1 cut(s) 163
EcoICRI GAGCTC 1 cut(s) 55
EcoT38I GRGCYC 1 cut(s) 57
FaeI CATG 1 cut(s) 263
FaiI YATR 2 cut(s) 34, 261
FatI CATG 1 cut(s) 259
FriOI GRGCYC 1 cut(s) 57
FspBI CTAG 2 cut(s) 165, 186
HaeIII GGCC 1 cut(s) 292
HgaI GACGC 1 cut(s) 138
Hin1I GRCGYC 1 cut(s) 130
Hin1II CATG 1 cut(s) 263
HinfI GANTC 3 cut(s) 13, 68, 230
Hpy166II GTNNAC 2 cut(s) 29, 209
Hpy188I TCNGA 1 cut(s) 182
Hpy188III TCNNGA 2 cut(s) 50, 186
Hpy8I GTNNAC 2 cut(s) 29, 209
HpyCH4III ACNGT 1 cut(s) 88
HpyCH4V TGCA 1 cut(s) 121
HpyF10VI GCNNNNNNNGC 2 cut(s) 153, 298
HpyF3I CTNAG 1 cut(s) 108
Hsp92I GRCGYC 1 cut(s) 130
Hsp92II CATG 1 cut(s) 263
Kzo9I GATC 4 cut(s) 157, 182, 193, 201
LmnI GCTCC 1 cut(s) 60
LpnPI CCDG 2 cut(s) 57, 252
MaeI CTAG 2 cut(s) 165, 186
MalI GATC 4 cut(s) 159, 184, 195, 203
MboI GATC 4 cut(s) 157, 182, 193, 201
MboII GAAGA 1 cut(s) 169
MflI RGATCY 1 cut(s) 182
MhlI GDGCHC 1 cut(s) 57
MluCI AATT 2 cut(s) 135, 253
MlyI GAGTC 3 cut(s) 22, 62, 224
MnlI CCTC 3 cut(s) 68, 215, 271
MwoI GCNNNNNNNGC 2 cut(s) 153, 298
NdeII GATC 4 cut(s) 157, 182, 193, 201
NlaIII CATG 1 cut(s) 263
NspI RCATGY 1 cut(s) 263
PciI ACATGT 1 cut(s) 259
Ple19I CGATCG 1 cut(s) 196
PleI GAGTC 3 cut(s) 21, 62, 224
PpsI GAGTC 3 cut(s) 21, 62, 224
PscI ACATGT 1 cut(s) 259
PshAI GACNNNNGTC 1 cut(s) 12
Psp124BI GAGCTC 1 cut(s) 57
PsuI RGATCY 1 cut(s) 182
PvuI CGATCG 1 cut(s) 196
RsaI GTAC 1 cut(s) 106
RsaNI GTAC 1 cut(s) 105
SacI GAGCTC 1 cut(s) 57
Sau3AI GATC 4 cut(s) 157, 182, 193, 201
SchI GAGTC 3 cut(s) 22, 62, 224
SduI GDGCHC 1 cut(s) 57
SetI ASST 2 cut(s) 48, 57
SmlI CTYRAG 1 cut(s) 10
SmoI CTYRAG 1 cut(s) 10
Sse9I AATT 2 cut(s) 135, 253
SsiI CCGC 1 cut(s) 5
SspMI CTAG 2 cut(s) 165, 186
SstI GAGCTC 1 cut(s) 57
TaaI ACNGT 1 cut(s) 88
TaqI TCGA 2 cut(s) 196, 228
TasI AATT 2 cut(s) 135, 253
TscAI CASTG 1 cut(s) 308
TspDTI ATGAA 1 cut(s) 231
TspRI CASTG 1 cut(s) 308
XapI RAATTY 1 cut(s) 253
XbaI TCTAGA 1 cut(s) 185
XceI RCATGY 1 cut(s) 263
XspI CTAG 2 cut(s) 165, 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.