MD11G1300400.v1.1

Monothiol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0004905
Physical Location & Seq
Forward (+)
41659872 .. 41660195
324 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1300400.v1.1.491

Sequence Viewer

Length: 324 bp
ATGGACACGATAACAAGCATGGTGGCCCAAAAGCCGGTGGTGATCTTCAGCAAGAGCTCTTGTTGCATGAGCCACACCATCAAGTCACTCATATCGAGCTACGGGGCAAACCCAACAGTGTATGAGCTAGATGAAATGCCAAATGGGCAGGCTATTGAAAGTGCACTAGTTCAGCAGCTCAAGTGCCAACCAAGTGTGCCGGCCGTTTTCATAGGGCAACAGTTTGTAGGCGGAGCTGATCGGGTCATGAGCCTCCAAGTCAGAAACCAGCTTGTCCCAATGCTCATAAGGTCCAATGCTATTTGGGTTTGGACTAGAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glutaredoxin

Protein Analysis

108

Amino Acids

11.78

Weight (kDa)

8.57

Isoelectric Point (pI)

43.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 13 - 76 2.6e-12 Glutaredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 231
AcoI YGGCCR 1 cut(s) 201
AcuI CTGAAG 1 cut(s) 31
AfiI CCNNNNNNNGG 1 cut(s) 34
AgsI TTSAA 1 cut(s) 158
AhlI ACTAGT 1 cut(s) 166
AluBI AGCT 6 cut(s) 57, 99, 127, 178, 236, 271
AluI AGCT 6 cut(s) 57, 99, 127, 178, 236, 271
Alw21I GWGCWC 2 cut(s) 59, 166
Alw44I GTGCAC 1 cut(s) 162
AoxI GGCC 2 cut(s) 24, 201
ApaLI GTGCAC 1 cut(s) 162
ApeKI GCWGC 1 cut(s) 175
AspS9I GGNCC 2 cut(s) 25, 291
AsuHPI GGTGA 1 cut(s) 52
AvaII GGWCC 1 cut(s) 291
BaeGI GKGCMC 1 cut(s) 166
BanII GRGCYC 1 cut(s) 59
Bbv12I GWGCWC 2 cut(s) 59, 166
BbvI GCAGC 1 cut(s) 187
BccI CCATC 1 cut(s) 86
BceAI ACGGC 1 cut(s) 188
BcuI ACTAGT 1 cut(s) 166
BfaI CTAG 3 cut(s) 128, 167, 315
BglI GCCNNNNNGGC 1 cut(s) 145
BisI GCNGC 1 cut(s) 176
BlsI GCNGC 1 cut(s) 177
Bme18I GGWCC 1 cut(s) 291
BmgT120I GGNCC 2 cut(s) 25, 291
BpuEI CTTGAG 1 cut(s) 164
Bsc4I CCNNNNNNNGG 1 cut(s) 34
Bse118I RCCGGY 2 cut(s) 34, 199
BseLI CCNNNNNNNGG 1 cut(s) 34
BseSI GKGCMC 1 cut(s) 166
BseX3I CGGCCG 1 cut(s) 201
BseXI GCAGC 1 cut(s) 187
Bsh1285I CGRYCG 1 cut(s) 204
BshFI GGCC 2 cut(s) 26, 203
BsiEI CGRYCG 1 cut(s) 204
BsiHKAI GWGCWC 2 cut(s) 59, 166
BsiSI CCGG 2 cut(s) 35, 200
BslFI GGGAC 1 cut(s) 260
BslI CCNNNNNNNGG 1 cut(s) 34
BsmFI GGGAC 1 cut(s) 260
BsnI GGCC 2 cut(s) 26, 203
Bsp1286I GDGCHC 2 cut(s) 59, 166
Bsp143I GATC 2 cut(s) 42, 238
BspACI CCGC 1 cut(s) 231
BspANI GGCC 2 cut(s) 26, 203
BspHI TCATGA 1 cut(s) 246
BsrFI RCCGGY 2 cut(s) 34, 199
BssAI RCCGGY 2 cut(s) 34, 199
BssMI GATC 2 cut(s) 42, 238
Bst4CI ACNGT 2 cut(s) 118, 222
BstC8I GCNNGC 2 cut(s) 150, 201
BstKTI GATC 2 cut(s) 45, 241
BstMBI GATC 2 cut(s) 42, 238
BstMCI CGRYCG 1 cut(s) 204
BstMWI GCNNNNNNNGC 2 cut(s) 63, 145
BstSLI GKGCMC 1 cut(s) 166
BstV1I GCAGC 1 cut(s) 187
BstZI CGGCCG 1 cut(s) 201
BsuRI GGCC 2 cut(s) 26, 203
BtsIMutI CAGTG 1 cut(s) 123
Cac8I GCNNGC 2 cut(s) 150, 201
CciI TCATGA 1 cut(s) 246
Cfr10I RCCGGY 2 cut(s) 34, 199
Cfr13I GGNCC 2 cut(s) 25, 291
CspCI CAANNNNNGTGG 1 cut(s) 38
CviAII CATG 3 cut(s) 19, 67, 247
DpnI GATC 2 cut(s) 44, 240
DpnII GATC 2 cut(s) 42, 238
EaeI YGGCCR 1 cut(s) 201
EagI CGGCCG 1 cut(s) 201
EciI GGCGGA 1 cut(s) 246
Ecl136II GAGCTC 1 cut(s) 57
EclXI CGGCCG 1 cut(s) 201
Eco24I GRGCYC 1 cut(s) 59
Eco47I GGWCC 1 cut(s) 291
Eco52I CGGCCG 1 cut(s) 201
Eco53kI GAGCTC 1 cut(s) 57
Eco57I CTGAAG 1 cut(s) 31
EcoICRI GAGCTC 1 cut(s) 57
EcoT38I GRGCYC 1 cut(s) 59
FaeI CATG 3 cut(s) 22, 70, 250
FaiI YATR 7 cut(s) 20, 68, 92, 123, 212, 248, 287
FaqI GGGAC 1 cut(s) 260
FatI CATG 3 cut(s) 18, 66, 246
Fnu4HI GCNGC 1 cut(s) 176
FriOI GRGCYC 1 cut(s) 59
Fsp4HI GCNGC 1 cut(s) 176
FspBI CTAG 3 cut(s) 128, 167, 315
GluI GCNGC 1 cut(s) 176
HaeIII GGCC 2 cut(s) 26, 203
HapII CCGG 2 cut(s) 35, 200
Hin1II CATG 3 cut(s) 22, 70, 250
HpaII CCGG 2 cut(s) 35, 200
HphI GGTGA 1 cut(s) 52
Hpy166II GTNNAC 1 cut(s) 164
Hpy188I TCNGA 1 cut(s) 263
Hpy188III TCNNGA 1 cut(s) 247
Hpy8I GTNNAC 1 cut(s) 164
HpyCH4III ACNGT 2 cut(s) 118, 222
HpyCH4V TGCA 2 cut(s) 66, 164
HpyF10VI GCNNNNNNNGC 2 cut(s) 63, 145
Hsp92II CATG 3 cut(s) 22, 70, 250
KroI GCCGGC 1 cut(s) 199
KroNI GCCGGC 1 cut(s) 201
Kzo9I GATC 2 cut(s) 42, 238
LmnI GCTCC 1 cut(s) 233
LpnPI CCDG 4 cut(s) 48, 134, 213, 281
Lsp1109I GCAGC 1 cut(s) 187
MaeI CTAG 3 cut(s) 128, 167, 315
MaeIII GTNAC 1 cut(s) 84
MalI GATC 2 cut(s) 44, 240
MboI GATC 2 cut(s) 42, 238
MboII GAAGA 1 cut(s) 37
MhlI GDGCHC 2 cut(s) 59, 166
MnlI CCTC 1 cut(s) 263
MroNI GCCGGC 1 cut(s) 199
MspI CCGG 2 cut(s) 35, 200
MwoI GCNNNNNNNGC 2 cut(s) 63, 145
NaeI GCCGGC 1 cut(s) 201
NdeII GATC 2 cut(s) 42, 238
NgoMIV GCCGGC 1 cut(s) 199
NlaIII CATG 3 cut(s) 22, 70, 250
NmuCI GTSAC 1 cut(s) 84
PagI TCATGA 1 cut(s) 246
PdiI GCCGGC 1 cut(s) 201
PkrI GCNGC 1 cut(s) 177
Psp124BI GAGCTC 1 cut(s) 59
PspPI GGNCC 2 cut(s) 25, 291
SacI GAGCTC 1 cut(s) 59
SatI GCNGC 1 cut(s) 176
Sau3AI GATC 2 cut(s) 42, 238
Sau96I GGNCC 2 cut(s) 25, 291
SduI GDGCHC 2 cut(s) 59, 166
SetI ASST 8 cut(s) 59, 101, 129, 180, 238, 273, 293, 323
SinI GGWCC 1 cut(s) 291
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
SpeI ACTAGT 1 cut(s) 166
SsiI CCGC 1 cut(s) 231
SspMI CTAG 3 cut(s) 128, 167, 315
SstI GAGCTC 1 cut(s) 59
TaaI ACNGT 2 cut(s) 118, 222
TaqI TCGA 1 cut(s) 95
TscAI CASTG 1 cut(s) 123
TseFI GTSAC 1 cut(s) 84
TseI GCWGC 1 cut(s) 175
Tsp45I GTSAC 1 cut(s) 84
TspDTI ATGAA 2 cut(s) 147, 199
TspRI CASTG 1 cut(s) 123
VneI GTGCAC 1 cut(s) 162
VpaK11BI GGWCC 1 cut(s) 291
XspI CTAG 3 cut(s) 128, 167, 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.