Rorug06G0230700

Monothiol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0004905
Physical Location & Seq
Reverse (-)
37158962 .. 37164921
5960 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0230700.1

Sequence Viewer

Length: 1377 bp
ATGGCATTTTGGTGCAGCAGACTCACCCAATCTAAGCTCAGGCAATTAGCCCATCACTCTAAGACCATTCCTTATGCTTCTTCTCTGCTTAACCCCACTGCTTCCAAGCCATGCGTTTCAGACAACCCATTGATCTCTCACCCTGATTTTATTTTTGCCAGTAAAGTTAGGTTCTTTGCTGCCCCAGTTCAGGCAAATGCAAATGCAAAGAAGACAGAGCAGGGCACGAGTGGTCCGCGGTTGAATGAGCATATCAGAGCCAATGTTGTTAGGCTTGTTATGGGCGAAGAACATTTTGTCATCTCAAAGAGTGAAGCTCTGGAACGTGCCAGGAATCTTGAACTTGATTTAGTCGAGGTTCAGGGCACAGCTAATCCTCCTGTTTGTAAAATCATGGATTACCATAAAGAGAAGTATAAAAAAGAAACAAGGGAGAAAGAGCAGATTAAAATCAAGTCTAAGGAAGTTAAGACTTTGCGTGCAGATACTAAGGAAGTCAAGTTTTCTCCAAAGACTGAGGCAAAGGATCTCAAGATGAAAGCAGACATGGTGAAGAGATTTATGGACAAGGGTTACCGGGTGAAGTGTACTGCCTCGGATTCTGAGGGTCGGGACTTGGGAGCAGTTTTCTCTCGTCTCATTGCGCTGATAGAAGACACAGCTTATATTGAATGTGAACCTACGGTAGGAAGAGGAAAAGAGTCATTCATAATGGTCAGGCATGTGAAATTTGGCCCACCAAAGAGTGGTGGGAAAAAAACTAAGGCTGAAAACAAAAAAAGCCCTGAGAAGGGTAGTGGTGATGGTATTGGGGTTACTACAAGAGCAGACACTGTTCCACTTCCTCCACAAGAATCTTCTGGTGTTGCTGAAAATAGGTATAGAAATACTGAGGTTTCACCAACAAGAGAAATGGATAGTAAGAGAGAGACTCCCAATAACAGAGAAATCCCAATTTCACATTTCTCACCATCAATGAGAGAGCAGCATATTCCATATCAAAGAAGAGAAGCGCCTACAAACGTGCACTTCTCATCGCCAACAAGAGAAACCAACAGAGCTGCTCCATCGGCATTTAGAAACTCTGCTCAGCTTCCTAACAGTATTCCCAAGCAAGAACCATCTAATCCTAATCCTAATCCTCCCCGTTCTGCAGGATTCAAGCAAGAACCATCTAGTCCTAATCCTCCTCGTTCTCGTCCTCCAGGACTTGGTTATGGGATATTTAGCACCCCAACTGGTAATGGTTCCGCAAAACAAGGTGTGTCGGAAAGGTTTCCGGCAAATCCAAACTCACCAAGTTCAAGACCTTATAGTAGCCAAAGACCAGGAACAGACATTGGTGAGGATGGACGGGGGATGGGGAATCTTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glutaredoxin

Protein Analysis

458

Amino Acids

50.77

Weight (kDa)

9.76

Isoelectric Point (pI)

47.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF3_N PF05198 82 - 148 1.1e-22 Translation initiation factor IF-3, N-terminal domain
IF3_C PF00707 162 - 233 4.1e-08 Translation initiation factor IF-3, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 746, 1211
AccII CGCG 1 cut(s) 238
AciI CCGC 3 cut(s) 236, 238, 1251
AclWI GGATC 1 cut(s) 534
AcsI RAATTY 1 cut(s) 728
AfaI GTAC 1 cut(s) 589
AfiI CCNNNNNNNGG 6 cut(s) 190, 686, 746, 790, 791, 1211
AgsI TTSAA 5 cut(s) 244, 341, 671, 1162, 1305
AjnI CCWGG 3 cut(s) 329, 1204, 1327
AluBI AGCT 6 cut(s) 37, 317, 371, 662, 1061, 1093
AluI AGCT 6 cut(s) 37, 317, 371, 662, 1061, 1093
Alw21I GWGCWC 1 cut(s) 1029
Alw26I GTCTC 2 cut(s) 641, 923
Alw44I GTGCAC 1 cut(s) 1025
AlwI GGATC 1 cut(s) 534
AlwNI CAGNNNCTG 1 cut(s) 833
AoxI GGCC 1 cut(s) 733
ApaLI GTGCAC 1 cut(s) 1025
ApeKI GCWGC 4 cut(s) 15, 179, 985, 1061
ApoI RAATTY 1 cut(s) 728
Asp700I GAANNNNTTC 1 cut(s) 1275
AspLEI GCGC 2 cut(s) 646, 1015
AspS9I GGNCC 2 cut(s) 233, 734
AsuC2I CCSGG 1 cut(s) 578
AsuHPI GGTGA 9 cut(s) 16, 131, 562, 592, 812, 891, 960, 1287, 1355
AvaII GGWCC 1 cut(s) 233
BaeGI GKGCMC 3 cut(s) 227, 368, 1029
BauI CACGAG 1 cut(s) 226
BbsI GAAGAC 2 cut(s) 218, 660
Bbv12I GWGCWC 1 cut(s) 1029
BbvI GCAGC 4 cut(s) 27, 166, 997, 1048
BccI CCATC 8 cut(s) 60, 797, 979, 1075, 1129, 1180, 1343, 1354
BciT130I CCWGG 3 cut(s) 331, 1206, 1329
BcnI CCSGG 1 cut(s) 578
BcoDI GTCTC 2 cut(s) 641, 923
BfaI CTAG 1 cut(s) 1176
BfmI CTRYAG 1 cut(s) 1152
BfoI RGCGCY 1 cut(s) 1016
BisI GCNGC 4 cut(s) 16, 180, 986, 1062
BlpI GCTNAGC 1 cut(s) 1089
BlsI GCNGC 4 cut(s) 17, 181, 987, 1063
Bme1390I CCNGG 4 cut(s) 331, 578, 1206, 1329
Bme18I GGWCC 1 cut(s) 233
BmgT120I GGNCC 2 cut(s) 233, 734
BmiI GGNNCC 1 cut(s) 1249
BmrFI CCNGG 4 cut(s) 331, 578, 1206, 1329
BmrI ACTGGG 1 cut(s) 179
BmuI ACTGGG 1 cut(s) 179
BpiI GAAGAC 2 cut(s) 218, 660
BplI GAGNNNNNCTC 4 cut(s) 301, 333, 916, 948
BpmI CTGGAG 1 cut(s) 1188
Bpu10I CCTNAGC 1 cut(s) 38
Bpu1102I GCTNAGC 1 cut(s) 1089
BpuEI CTTGAG 1 cut(s) 515
BpuMI CCSGG 1 cut(s) 578
BsaBI GATNNNNATC 1 cut(s) 449
BsaJI CCNNGG 2 cut(s) 236, 594
Bsc4I CCNNNNNNNGG 6 cut(s) 190, 686, 746, 790, 791, 1211
Bse1I ACTGG 3 cut(s) 159, 185, 1243
Bse3DI GCAATG 1 cut(s) 639
Bse8I GATNNNNATC 1 cut(s) 449
BseBI CCWGG 3 cut(s) 331, 1206, 1329
BseDI CCNNGG 2 cut(s) 236, 594
BseGI GGATG 2 cut(s) 1354, 1365
BseJI GATNNNNATC 1 cut(s) 449
BseLI CCNNNNNNNGG 6 cut(s) 190, 686, 746, 790, 791, 1211
BseMI GCAATG 1 cut(s) 639
BseMII CTCAG 6 cut(s) 52, 507, 594, 777, 882, 1103
BseNI ACTGG 3 cut(s) 159, 185, 1243
BseRI GAGGAG 1 cut(s) 1179
BseSI GKGCMC 3 cut(s) 227, 368, 1029
BseXI GCAGC 4 cut(s) 27, 166, 997, 1048
BsgI GTGCAG 2 cut(s) 34, 501
Bsh1236I CGCG 1 cut(s) 238
BshFI GGCC 1 cut(s) 735
BsiHKAI GWGCWC 1 cut(s) 1029
BsiSI CCGG 2 cut(s) 577, 1280
BslFI GGGAC 1 cut(s) 626
BslI CCNNNNNNNGG 6 cut(s) 190, 686, 746, 790, 791, 1211
BsmAI GTCTC 2 cut(s) 641, 923
BsmBI CGTCTC 1 cut(s) 641
BsmFI GGGAC 1 cut(s) 626
BsnI GGCC 1 cut(s) 735
Bsp1286I GDGCHC 3 cut(s) 227, 368, 1029
Bsp143I GATC 2 cut(s) 132, 526
Bsp1720I GCTNAGC 1 cut(s) 1089
BspACI CCGC 3 cut(s) 236, 238, 1251
BspANI GGCC 1 cut(s) 735
BspCNI CTCAG 6 cut(s) 51, 508, 595, 778, 883, 1102
BspFNI CGCG 1 cut(s) 238
BspLI GGNNCC 1 cut(s) 1249
BspMAI CTGCAG 1 cut(s) 1156
BspPI GGATC 1 cut(s) 534
BsrDI GCAATG 1 cut(s) 639
BsrI ACTGG 3 cut(s) 159, 185, 1243
BssECI CCNNGG 2 cut(s) 236, 594
BssMI GATC 2 cut(s) 132, 526
BssSI CACGAG 1 cut(s) 226
Bst2BI CACGAG 1 cut(s) 226
Bst2UI CCWGG 3 cut(s) 331, 1206, 1329
Bst4CI ACNGT 3 cut(s) 685, 835, 1103
Bst6I CTCTTC 3 cut(s) 548, 685, 1000
BstC8I GCNNGC 1 cut(s) 480
BstDSI CCRYGG 1 cut(s) 236
BstEII GGTNACC 1 cut(s) 572
BstENI CCTNNNNNAGG 1 cut(s) 684
BstF5I GGATG 2 cut(s) 1354, 1365
BstFNI CGCG 1 cut(s) 238
BstH2I RGCGCY 1 cut(s) 1016
BstHHI GCGC 2 cut(s) 646, 1015
BstKTI GATC 2 cut(s) 135, 529
BstMAI GTCTC 2 cut(s) 641, 923
BstMBI GATC 2 cut(s) 132, 526
BstMWI GCNNNNNNNGC 1 cut(s) 1070
BstNI CCWGG 3 cut(s) 331, 1206, 1329
BstNSI RCATGY 1 cut(s) 725
BstPI GGTNACC 1 cut(s) 572
BstSCI CCNGG 4 cut(s) 329, 576, 1204, 1327
BstSFI CTRYAG 1 cut(s) 1152
BstSLI GKGCMC 3 cut(s) 227, 368, 1029
BstUI CGCG 1 cut(s) 238
BstV1I GCAGC 4 cut(s) 27, 166, 997, 1048
BstV2I GAAGAC 2 cut(s) 218, 660
BstX2I RGATCY 1 cut(s) 526
BstYI RGATCY 1 cut(s) 526
BsuRI GGCC 1 cut(s) 735
BtgI CCRYGG 1 cut(s) 236
BtgZI GCGATG 1 cut(s) 1020
BtsCI GGATG 2 cut(s) 1354, 1365
BtsI GCAGTG 1 cut(s) 96
BtsIMutI CAGTG 2 cut(s) 96, 831
Cac8I GCNNGC 1 cut(s) 480
CaiI CAGNNNCTG 1 cut(s) 833
CfoI GCGC 2 cut(s) 646, 1015
Cfr13I GGNCC 2 cut(s) 233, 734
Cfr42I CCGCGG 1 cut(s) 239
Csp6I GTAC 1 cut(s) 588
CviAII CATG 4 cut(s) 111, 394, 547, 722
CviQI GTAC 1 cut(s) 588
DpnI GATC 2 cut(s) 134, 528
DpnII GATC 2 cut(s) 132, 526
Eam1104I CTCTTC 3 cut(s) 548, 685, 1000
EarI CTCTTC 3 cut(s) 548, 685, 1000
Eco47I GGWCC 1 cut(s) 233
Eco91I GGTNACC 1 cut(s) 572
EcoNI CCTNNNNNAGG 1 cut(s) 684
EcoO65I GGTNACC 1 cut(s) 572
EcoRII CCWGG 3 cut(s) 329, 1204, 1327
Esp3I CGTCTC 1 cut(s) 641
FaeI CATG 4 cut(s) 114, 397, 550, 725
FaqI GGGAC 1 cut(s) 626
FatI CATG 4 cut(s) 110, 393, 546, 721
Fnu4HI GCNGC 4 cut(s) 16, 180, 986, 1062
FokI GGATG 2 cut(s) 1361, 1372
Fsp4HI GCNGC 4 cut(s) 16, 180, 986, 1062
FspBI CTAG 1 cut(s) 1176
GlaI GCGC 2 cut(s) 645, 1014
GluI GCNGC 4 cut(s) 16, 180, 986, 1062
GsuI CTGGAG 1 cut(s) 1188
HaeII RGCGCY 1 cut(s) 1016
HaeIII GGCC 1 cut(s) 735
HapII CCGG 2 cut(s) 577, 1280
HhaI GCGC 2 cut(s) 646, 1015
Hin1II CATG 4 cut(s) 114, 397, 550, 725
Hin6I GCGC 2 cut(s) 644, 1013
HinP1I GCGC 2 cut(s) 644, 1013
HinfI GANTC 8 cut(s) 21, 334, 599, 701, 854, 931, 1158, 1366
HpaII CCGG 2 cut(s) 577, 1280
HphI GGTGA 9 cut(s) 16, 131, 562, 592, 812, 891, 960, 1287, 1355
Hpy166II GTNNAC 3 cut(s) 588, 677, 1027
Hpy188I TCNGA 5 cut(s) 121, 257, 598, 604, 1270
Hpy188III TCNNGA 5 cut(s) 320, 338, 532, 611, 1305
Hpy8I GTNNAC 3 cut(s) 588, 677, 1027
HpyAV CCTTC 1 cut(s) 784
HpyCH4III ACNGT 3 cut(s) 685, 835, 1103
HpyCH4IV ACGT 2 cut(s) 325, 1023
HpyCH4V TGCA 6 cut(s) 15, 200, 206, 482, 1027, 1154
HpyF10VI GCNNNNNNNGC 1 cut(s) 1070
HpySE526I ACGT 2 cut(s) 325, 1023
Hsp92II CATG 4 cut(s) 114, 397, 550, 725
HspAI GCGC 2 cut(s) 644, 1013
KspI CCGCGG 1 cut(s) 239
Kzo9I GATC 2 cut(s) 132, 526
LmnI GCTCC 2 cut(s) 620, 1069
Lsp1109I GCAGC 4 cut(s) 27, 166, 997, 1048
MaeI CTAG 1 cut(s) 1176
MaeII ACGT 2 cut(s) 325, 1023
MaeIII GTNAC 2 cut(s) 572, 814
MalI GATC 2 cut(s) 134, 528
MboI GATC 2 cut(s) 132, 526
MboII GAAGA 8 cut(s) 72, 223, 299, 565, 665, 702, 849, 1017
MflI RGATCY 1 cut(s) 526
MhlI GDGCHC 3 cut(s) 227, 368, 1029
MluCI AATT 3 cut(s) 44, 728, 954
MlyI GAGTC 3 cut(s) 15, 710, 925
MmeI TCCRAC 1 cut(s) 1248
MroXI GAANNNNTTC 1 cut(s) 1275
MseI TTAA 3 cut(s) 90, 447, 468
MslI CAYNNNNRTG 1 cut(s) 10
MspA1I CMGCKG 1 cut(s) 238
MspI CCGG 2 cut(s) 577, 1280
MspR9I CCNGG 4 cut(s) 331, 578, 1206, 1329
MvaI CCWGG 3 cut(s) 331, 1206, 1329
MvnI CGCG 1 cut(s) 238
MwoI GCNNNNNNNGC 1 cut(s) 1070
NciI CCSGG 1 cut(s) 578
NdeII GATC 2 cut(s) 132, 526
NlaIII CATG 4 cut(s) 114, 397, 550, 725
NlaIV GGNNCC 1 cut(s) 1249
NspI RCATGY 1 cut(s) 725
PdmI GAANNNNTTC 1 cut(s) 1275
PfeI GAWTC 5 cut(s) 334, 599, 854, 1158, 1366
PflMI CCANNNNNTGG 2 cut(s) 746, 1211
PfoI TCCNGGA 1 cut(s) 1204
PkrI GCNGC 4 cut(s) 17, 181, 987, 1063
PleI GAGTC 3 cut(s) 15, 709, 925
PpsI GAGTC 3 cut(s) 15, 709, 925
Psp6I CCWGG 3 cut(s) 329, 1204, 1327
PspEI GGTNACC 1 cut(s) 572
PspGI CCWGG 3 cut(s) 329, 1204, 1327
PspN4I GGNNCC 1 cut(s) 1249
PspPI GGNCC 2 cut(s) 233, 734
PstI CTGCAG 1 cut(s) 1156
PstNI CAGNNNCTG 1 cut(s) 833
PsuI RGATCY 1 cut(s) 526
RsaI GTAC 1 cut(s) 589
RsaNI GTAC 1 cut(s) 588
RseI CAYNNNNRTG 1 cut(s) 10
SacII CCGCGG 1 cut(s) 239
SaqAI TTAA 3 cut(s) 90, 447, 468
SatI GCNGC 4 cut(s) 16, 180, 986, 1062
Sau3AI GATC 2 cut(s) 132, 526
Sau96I GGNCC 2 cut(s) 233, 734
SchI GAGTC 3 cut(s) 15, 710, 925
ScrFI CCNGG 4 cut(s) 331, 578, 1206, 1329
SduI GDGCHC 3 cut(s) 227, 368, 1029
SfcI CTRYAG 1 cut(s) 1152
Sfr303I CCGCGG 1 cut(s) 239
SgrBI CCGCGG 1 cut(s) 239
SinI GGWCC 1 cut(s) 233
SmiMI CAYNNNNRTG 1 cut(s) 10
SmlI CTYRAG 1 cut(s) 530
SmoI CTYRAG 1 cut(s) 530
Sse9I AATT 3 cut(s) 44, 728, 954
SsiI CCGC 3 cut(s) 236, 238, 1251
SspMI CTAG 1 cut(s) 1176
StyD4I CCNGG 4 cut(s) 329, 576, 1204, 1327
TaaI ACNGT 3 cut(s) 685, 835, 1103
TaiI ACGT 2 cut(s) 328, 1026
TaqI TCGA 1 cut(s) 354
TasI AATT 3 cut(s) 44, 728, 954
TatI WGTACW 1 cut(s) 587
TfiI GAWTC 5 cut(s) 334, 599, 854, 1158, 1366
Tru1I TTAA 3 cut(s) 90, 447, 468
Tru9I TTAA 3 cut(s) 90, 447, 468
TscAI CASTG 2 cut(s) 103, 838
TseI GCWGC 4 cut(s) 15, 179, 985, 1061
TspDTI ATGAA 2 cut(s) 551, 697
TspRI CASTG 2 cut(s) 103, 838
Van91I CCANNNNNTGG 2 cut(s) 746, 1211
VneI GTGCAC 1 cut(s) 1025
VpaK11BI GGWCC 1 cut(s) 233
XagI CCTNNNNNAGG 1 cut(s) 684
XapI RAATTY 1 cut(s) 728
XceI RCATGY 1 cut(s) 725
XmnI GAANNNNTTC 1 cut(s) 1275
XspI CTAG 1 cut(s) 1176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.