Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0010429

Physical Location & Seq

Genomic Coordinates Forward (+)

493753 .. 497567

Gene Structure 3815 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1003200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2166 bp

ATGATGCTTCTGCCAGTGCCAGGTTCGACTTGCTTCCCTTCCATTCAATTGGCTTCTCCGACACTCATCCGCCACCACCACCATGTCTGGCCTCTGTCCGCCACCGCAGCCGCTGCCGTCACCAATTCATCCGAACCCCATCTGCCCCCAATTCCATCTTCCTCAACTACGATTCATTTAACCTCAACCTCGAGACGACGCCGCTTTGGGGACGACCAAGCTGCCGCTTCCCCCTACGACTTCGGTCCCCTCCTCACCTTCCTCGCCAACTCATTGTCATCCGATAATGATTCCGCCTCCTCCTCTTCCTCTTCGTCCTCCTCCTCCTCTTCCTCTTCGTCCTCCTCCTCCTCCACCTCCTCTCCAACCTCACTCGACCCGTCCGAGTTCCAGCTGGCCCAGTCCTACCGCGCTGTCCCTGCTCCACTCTGGCACTCCCTCCTCAAGTCCCTCTGCGCCTCCCACTCCTCCTCCGACATTGGACTTGCCTACGCCGTCGTTTCGTGGCTCCAAAAGCACAACCTCTGCTTCTCCTACGAACTCCTCTACTCCATCCTCATCCACGCCCTCGGCCGCTCCGAGAAGCTCTACGAGGCTTTCTTGCTCTCCCAGCGCCAATCCCTCACTCCCCTCACCTACAATGCTCTCATCGGCGCCTGTGCTCGCAACGGCGACCTCGAGAAGGCACTCCATTTGATGTCCCGCATGCGCCAGGACGGTTACCGCTCCGACTTCGTCAACTACAGCTTGATCATTCAGTCCCTCACCCGCTCCAATAAGATTGATTCCCCAATCATGTTCAAGCTCTACAGGGAGATTGAGTCCGAGAGCATTGAGATTGATGGCCAGCTCTTCAACGACATCATTGCTGGTTTCGCCAAAGCCGGCGAGCCCTCCCAGGCTGTGCACCTTCTGGCCATGGTCCAGGCCACTGGCTTGAGCCCCAAAACTGCCACTCTGGTTGCTGTCATCTCAGCCTTGGGGAATTCCGGCAGGGTCGTTGAAGCCGAAGCTATCTTTGAGGAAATGAGAGAAGGAGGATTGCAACCCAGGACTAGGGCTTACAATGCCCTTCTCAAAGGCTATGTGAAGGCAGGTCAACTGAAAGATGCCGAATCCATTGTGTCCCAGATGGAGAAGAGCGGTATTTCTCCTGATGAGCACACTTACAGCCTGCTCATTGATGCCTATGCGAATGCAGGTAGGTGGGAAAGCGCAAGAATTGTGTTGAAAGAGATGGAAGCTAGCAATGTGCAGCCTAATTCCTATGTTTTCAGCAGGATTTTAGCCAGCTACCGTGATAGAGGGGAGTGGCAAAAGTCGTTCCAAGTTTTGAGGGAGATGAAGAGCAGTGGAGTAAGACCCGATAGGCATTTTTACAATGTCATGATTGACACTTTTGGAAAGTCCAACTGTCTTGATCATGCTATGGCTACCTTTGAAAGGATGCTATCTGAGGGAATCCACCCGGACACCGTCACCTGGAATACGCTTATAGATTGTCATTGCAAGTCAGGCCACCATGTAAGGGCGGAGGAGTTGTTTGAGGAAATGCAGCAAAGTGGGTGCGCCCCTTGCGCCACCACCTATAACATCATGATCAATTCCTTTGGGGAGCAGCAAAGATGGGATGAGGTGAAAGGCTTGTTGGGGAAGATGCAGTCTCAGGGTTTACTGCCTAACATAGTTACCTATACCACATTAGTTGACATTTATGGAAAGTCGGGGAGATTTACTGATGCAATAGAGTGTTTGGAGGTTATGAAGTCTGCAGGCATGAAGCCATCCCCGACGATGTACAATGCCCTAATTAATGCCTATGCACAAAGAGGTTTGTCAGAGCAAGCACTGAACGCGTTTAGGGTAATGAGAGCAGATGGATTAAAACCCAGTCCCTTGGCTCTCAATTCGTTAATCAACGCATTTGGTGAAGATAGAAGGGATACTGAGGCCTTTTCTGTGTTGCAGTACATGAAGGAAAATGACCTGAAACCAGATGTAGTTACGTATACTACACTTATGAAAACCCTTATCCGTGTTGATAAATTTTGTAAGGTTCCAGCGGTGTATGAAGAAATGATTATGTCTGGCTGCACTCCAGATAGGAAAGCGAGGGCAATGTTACGGTCTGCGCTGAAATACATGAAACAGACACTCAGATCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003729 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006417 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009295 GO:0009451 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009657 GO:0009658 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010239 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0016020 GO:0016043 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031425 GO:0032268 GO:0032270 GO:0034248 GO:0034250 GO:0034357 GO:0034641 GO:0042644 GO:0042646 GO:0042651 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0045727 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0060255 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363 GO:2000112

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

722

Amino Acids

79.71

Weight (kDa)

7.03

Isoelectric Point (pI)

55.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_24	PF23276	203 - 304	1.4e-07	Fungal tetratrico peptide repeats
PPR_2	PF13041	212 - 255	1.1e-07	PPR repeat family
PPR	PF01535	212 - 240	8e-08	PPR repeat
PPR_long	PF17177	213 - 367	4.3e-10	Pentacotripeptide-repeat region of PRORP
PPR_long	PF17177	262 - 402	9.7e-13	Pentacotripeptide-repeat region of PRORP
TPR_24	PF23276	273 - 373	9.1e-06	Fungal tetratrico peptide repeats
PPR_3	PF13812	339 - 399	4.7e-12	Pentatricopeptide repeat domain
PPR_2	PF13041	352 - 399	5.7e-15	PPR repeat family
PPR	PF01535	354 - 383	1.9e-06	PPR repeat
PPR_long	PF17177	371 - 457	1.9e-10	Pentacotripeptide-repeat region of PRORP
TPR_24	PF23276	372 - 482	1.3e-11	Fungal tetratrico peptide repeats
PPR_1	PF12854	382 - 414	4.5e-06	PPR repeat
PPR_3	PF13812	409 - 469	2.5e-09	Pentatricopeptide repeat domain
PPR_long	PF17177	460 - 615	2.9e-10	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	460 - 494	8.1e-06	PPR repeat family
PPR_3	PF13812	481 - 539	5e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	487 - 519	1.1e-11	PPR repeat
PPR_2	PF13041	490 - 537	4.4e-17	PPR repeat family
PPR	PF01535	493 - 523	3.4e-10	PPR repeat
PPR_2	PF13041	525 - 570	3.3e-10	PPR repeat family
PPR_3	PF13812	549 - 609	1.1e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	557 - 589	4.1e-07	PPR repeat
PPR_2	PF13041	560 - 596	2.6e-07	PPR repeat family
PPR	PF01535	563 - 592	8e-06	PPR repeat
PPR_3	PF13812	592 - 644	4.5e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	598 - 642	4.1e-07	PPR repeat family
TPR_24	PF23276	600 - 691	1.1e-07	Fungal tetratrico peptide repeats
PPR_3	PF13812	621 - 676	4.3e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	635 - 677	1.3e-09	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0010429)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G42310
fragaria_vesca	FvH4_2g37460 FvH4_2g37460 FvH4_2g37460 FvH4_5g30181
malus_domestica	MD12G1003200.v1.1 MD15G1002100.v1.1
prunus_persica	Prupe.1G354600_v2.0.a1
pyrus_communis	pycom08g00200 pycom15g00100
rosa_chinensis	RchiOBHm_Chr6g0304261
rosa_laevigata	RLG00000010997
rosa_multiflora	Rmu_co8272487.1_g000001 Rmu_ssc0000218.1_g000026
rosa_roxburghii	Rroxscaffold_7G00163900
rosa_rugosa	Rorug06G0329300
rosa_samantha	Rh6AG442900 Rh6BG459600 Rh6CG455600 Rh6DG442400
rosa_wichuraiana	Rw6G038420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1085, 1190
AccB1I	GGYRCC	1 cut(s)	653
AccBSI	CCGCTC	4 cut(s)	576, 726, 771, 1143
AccI	GTMKAC	1 cut(s)	2009
AccII	CGCG	2 cut(s)	411, 1856
AclWI	GGATC	1 cut(s)	2154
AcoI	YGGCCR	3 cut(s)	571, 844, 915
AcsI	RAATTY	2 cut(s)	985, 2045
AcyI	GRCGYC	2 cut(s)	199, 654
AfaI	GTAC	2 cut(s)	1799, 1970
AfiI	CCNNNNNNNGG	7 cut(s)	20, 208, 682, 1056, 1443, 1482, 1528
AflIII	ACRYGT	1 cut(s)	1854
AgsI	TTSAA	6 cut(s)	47, 802, 856, 1004, 1231, 1442
AjnI	CCWGG	6 cut(s)	19, 711, 897, 924, 1049, 1481
AjuI	GAANNNNNNNTTGG	4 cut(s)	142, 174, 1631, 1663
AluBI	AGCT	9 cut(s)	221, 394, 586, 747, 805, 850, 1013, 1244, 1293
AluI	AGCT	9 cut(s)	221, 394, 586, 747, 805, 850, 1013, 1244, 1293
Alw21I	GWGCWC	3 cut(s)	664, 909, 1164
Alw26I	GTCTC	2 cut(s)	187, 1668
Alw44I	GTGCAC	1 cut(s)	905
AlwI	GGATC	1 cut(s)	2154
AlwNI	CAGNNNCTG	1 cut(s)	113
Ama87I	CYCGRG	2 cut(s)	190, 677
AoxI	GGCC	8 cut(s)	89, 396, 571, 844, 915, 927, 1516, 1950
ApaLI	GTGCAC	1 cut(s)	905
ApeKI	GCWGC	7 cut(s)	107, 113, 221, 1255, 1555, 1618, 2091
ApoI	RAATTY	2 cut(s)	985, 2045
AseI	ATTAAT	1 cut(s)	1812
AspLEI	GCGC	9 cut(s)	413, 458, 615, 656, 711, 1217, 1571, 1580, 2134
AspS9I	GGNCC	3 cut(s)	245, 397, 922
AsuC2I	CCSGG	1 cut(s)	1469
AsuHPI	GGTGA	7 cut(s)	112, 247, 625, 757, 1471, 1648, 1940
AsuNHI	GCTAGC	1 cut(s)	1244
AvaI	CYCGRG	2 cut(s)	190, 677
AvaII	GGWCC	2 cut(s)	245, 922
BaeGI	GKGCMC	1 cut(s)	909
BalI	TGGCCA	2 cut(s)	846, 917
BanI	GGYRCC	1 cut(s)	653
BanII	GRGCYC	2 cut(s)	894, 944
Bbv12I	GWGCWC	3 cut(s)	664, 909, 1164
BbvI	GCAGC	7 cut(s)	100, 119, 208, 1267, 1567, 1630, 2078
BccI	CCATC	9 cut(s)	147, 163, 560, 836, 1126, 1231, 1620, 1792, 1871
BceAI	ACGGC	3 cut(s)	101, 479, 685
BcgI	CGANNNNNNTGC	4 cut(s)	203, 237, 848, 882
BciT130I	CCWGG	6 cut(s)	21, 713, 899, 926, 1051, 1483
BciVI	GTATCC	1 cut(s)	1936
BclI	TGATCA	3 cut(s)	750, 1420, 1599
BcnI	CCSGG	1 cut(s)	1469
BcoDI	GTCTC	2 cut(s)	187, 1668
BfaI	CTAG	2 cut(s)	1056, 1245
BfmI	CTRYAG	3 cut(s)	742, 808, 1770
BfoI	RGCGCY	2 cut(s)	616, 657
BfuAI	ACCTGC	2 cut(s)	1085, 1190
BfuI	GTATCC	1 cut(s)	1936
Bme1390I	CCNGG	7 cut(s)	21, 713, 899, 926, 1051, 1469, 1483
Bme18I	GGWCC	2 cut(s)	245, 922
BmeT110I	CYCGRG	2 cut(s)	190, 677
BmgT120I	GGNCC	3 cut(s)	245, 397, 922
BmiI	GGNNCC	4 cut(s)	247, 509, 655, 2058
BmrFI	CCNGG	7 cut(s)	21, 713, 899, 926, 1051, 1469, 1483
BmrI	ACTGGG	2 cut(s)	394, 1884
BmsI	GCATC	5 cut(s)	1099, 1174, 1437, 1647, 1729
BmtI	GCTAGC	1 cut(s)	1248
BmuI	ACTGGG	2 cut(s)	394, 1884
BoxI	GACNNNNGTC	1 cut(s)	243
BpmI	CTGGAG	1 cut(s)	2082
BpuEI	CTTGAG	2 cut(s)	428, 958
BpuMI	CCSGG	1 cut(s)	1469
BsaAI	YACGTR	1 cut(s)	2007
BsaHI	GRCGYC	2 cut(s)	199, 654
BsaJI	CCNNGG	6 cut(s)	568, 897, 918, 978, 1049, 1896
BsaXI	ACNNNNNCTCC	8 cut(s)	346, 376, 455, 485, 806, 836, 1526, 1556
Bsc4I	CCNNNNNNNGG	7 cut(s)	20, 208, 682, 1056, 1443, 1482, 1528
Bse118I	RCCGGY	1 cut(s)	884
Bse1I	ACTGG	4 cut(s)	14, 400, 937, 1890
Bse3DI	GCAATG	4 cut(s)	864, 1255, 1504, 2124
BseBI	CCWGG	6 cut(s)	21, 713, 899, 926, 1051, 1483
BseDI	CCNNGG	6 cut(s)	568, 897, 918, 978, 1049, 1896
BseGI	GGATG	8 cut(s)	66, 128, 278, 552, 558, 1452, 1636, 1784
BseLI	CCNNNNNNNGG	7 cut(s)	20, 208, 682, 1056, 1443, 1482, 1528
BseMI	GCAATG	4 cut(s)	864, 1255, 1504, 2124
BseMII	CTCAG	4 cut(s)	987, 1446, 1679, 1938
BseNI	ACTGG	4 cut(s)	14, 400, 937, 1890
BseSI	GKGCMC	1 cut(s)	909
BseX3I	CGGCCG	1 cut(s)	571
BseXI	GCAGC	7 cut(s)	100, 119, 208, 1267, 1567, 1630, 2078
BseYI	CCCAGC	1 cut(s)	609
BsgI	GTGCAG	2 cut(s)	1274, 2077
Bsh1236I	CGCG	2 cut(s)	411, 1856
Bsh1285I	CGRYCG	1 cut(s)	574
BshFI	GGCC	8 cut(s)	91, 398, 573, 846, 917, 929, 1518, 1952
BshNI	GGYRCC	1 cut(s)	653
BsiEI	CGRYCG	1 cut(s)	574
BsiHKAI	GWGCWC	3 cut(s)	664, 909, 1164
BsiHKCI	CYCGRG	2 cut(s)	190, 677
BsiSI	CCGG	3 cut(s)	885, 990, 1469
BslFI	GGGAC	8 cut(s)	224, 231, 401, 433, 685, 745, 1111, 1878
BslI	CCNNNNNNNGG	7 cut(s)	20, 208, 682, 1056, 1443, 1482, 1528
BsmAI	GTCTC	2 cut(s)	187, 1668
BsmBI	CGTCTC	1 cut(s)	187
BsmFI	GGGAC	8 cut(s)	224, 231, 401, 433, 685, 745, 1111, 1878
BsmI	GAATGC	1 cut(s)	1201
BsnI	GGCC	8 cut(s)	91, 398, 573, 846, 917, 929, 1518, 1952
BsoBI	CYCGRG	2 cut(s)	190, 677
Bsp1286I	GDGCHC	5 cut(s)	664, 894, 909, 944, 1164
Bsp1407I	TGTACA	1 cut(s)	1797
Bsp143I	GATC	4 cut(s)	750, 1420, 1599, 2159
Bsp19I	CCATGG	1 cut(s)	918
BspANI	GGCC	8 cut(s)	91, 398, 573, 846, 917, 929, 1518, 1952
BspCNI	CTCAG	4 cut(s)	986, 1447, 1678, 1939
BspFNI	CGCG	2 cut(s)	411, 1856
BspHI	TCATGA	2 cut(s)	1386, 1596
BspLI	GGNNCC	4 cut(s)	247, 509, 655, 2058
BspMAI	CTGCAG	1 cut(s)	1774
BspMI	ACCTGC	2 cut(s)	1085, 1190
BspOI	GCTAGC	1 cut(s)	1248
BspPI	GGATC	1 cut(s)	2154
BspQI	GCTCTTC	3 cut(s)	857, 1133, 1340
BspT107I	GGYRCC	1 cut(s)	653
BsrBI	CCGCTC	4 cut(s)	576, 726, 771, 1143
BsrDI	GCAATG	4 cut(s)	864, 1255, 1504, 2124
BsrFI	RCCGGY	1 cut(s)	884
BsrGI	TGTACA	1 cut(s)	1797
BsrI	ACTGG	4 cut(s)	14, 400, 937, 1890
BssAI	RCCGGY	1 cut(s)	884
BssECI	CCNNGG	6 cut(s)	568, 897, 918, 978, 1049, 1896
BssMI	GATC	4 cut(s)	750, 1420, 1599, 2159
BssNAI	GTATAC	1 cut(s)	2010
BssNI	GRCGYC	2 cut(s)	199, 654
BssT1I	CCWWGG	3 cut(s)	918, 978, 1896
Bst1107I	GTATAC	1 cut(s)	2010
Bst2UI	CCWGG	6 cut(s)	21, 713, 899, 926, 1051, 1483
Bst4CI	ACNGT	5 cut(s)	719, 1298, 1415, 1477, 2127
Bst6I	CTCTTC	7 cut(s)	310, 316, 334, 340, 857, 1133, 1340
BstACI	GRCGYC	2 cut(s)	199, 654
BstAPI	GCANNNNNTGC	1 cut(s)	113
BstAUI	TGTACA	1 cut(s)	1797
BstBAI	YACGTR	1 cut(s)	2007
BstDEI	CTNAG	5 cut(s)	973, 1455, 1665, 1947, 2156
BstDSI	CCRYGG	1 cut(s)	918
BstEII	GGTNACC	1 cut(s)	719
BstENI	CCTNNNNNAGG	2 cut(s)	680, 1441
BstF5I	GGATG	8 cut(s)	66, 128, 278, 552, 558, 1452, 1636, 1784
BstFNI	CGCG	2 cut(s)	411, 1856
BstH2I	RGCGCY	2 cut(s)	616, 657
BstHHI	GCGC	9 cut(s)	413, 458, 615, 656, 711, 1217, 1571, 1580, 2134
BstKTI	GATC	4 cut(s)	753, 1423, 1602, 2162
BstMAI	GTCTC	2 cut(s)	187, 1668
BstMBI	GATC	4 cut(s)	750, 1420, 1599, 2159
BstMCI	CGRYCG	1 cut(s)	574
BstNI	CCWGG	6 cut(s)	21, 713, 899, 926, 1051, 1483
BstNSI	RCATGY	1 cut(s)	709
BstPAI	GACNNNNGTC	1 cut(s)	243
BstPI	GGTNACC	1 cut(s)	719
BstSCI	CCNGG	7 cut(s)	19, 711, 897, 924, 1049, 1467, 1481
BstSFI	CTRYAG	3 cut(s)	742, 808, 1770
BstSLI	GKGCMC	1 cut(s)	909
BstSNI	TACGTA	1 cut(s)	2007
BstUI	CGCG	2 cut(s)	411, 1856
BstV1I	GCAGC	7 cut(s)	100, 119, 208, 1267, 1567, 1630, 2078
BstX2I	RGATCY	1 cut(s)	2159
BstXI	CCANNNNNNTGG	3 cut(s)	49, 932, 1897
BstYI	RGATCY	1 cut(s)	2159
BstZ17I	GTATAC	1 cut(s)	2010
BstZI	CGGCCG	1 cut(s)	571
BsuI	GTATCC	1 cut(s)	1936
BsuRI	GGCC	8 cut(s)	91, 398, 573, 846, 917, 929, 1518, 1952
BtgI	CCRYGG	1 cut(s)	918
BtsCI	GGATG	8 cut(s)	66, 128, 278, 552, 558, 1452, 1636, 1784
BtsI	GCAGTG	1 cut(s)	1357
BtsIMutI	CAGTG	4 cut(s)	21, 930, 1357, 1847
BveI	ACCTGC	2 cut(s)	1085, 1190
CaiI	CAGNNNCTG	1 cut(s)	113
CciI	TCATGA	2 cut(s)	1386, 1596
CfoI	GCGC	9 cut(s)	413, 458, 615, 656, 711, 1217, 1571, 1580, 2134
Cfr10I	RCCGGY	1 cut(s)	884
Cfr13I	GGNCC	3 cut(s)	245, 397, 922
CseI	GACGC	1 cut(s)	207
Csp6I	GTAC	2 cut(s)	1798, 1969
CspCI	CAANNNNNGTGG	4 cut(s)	943, 978, 1687, 1722
CviQI	GTAC	2 cut(s)	1798, 1969
DdeI	CTNAG	5 cut(s)	973, 1455, 1665, 1947, 2156
DinI	GGCGCC	1 cut(s)	655
DpnI	GATC	4 cut(s)	752, 1422, 1601, 2161
DpnII	GATC	4 cut(s)	750, 1420, 1599, 2159
EaeI	YGGCCR	3 cut(s)	571, 844, 915
EagI	CGGCCG	1 cut(s)	571
Eam1104I	CTCTTC	7 cut(s)	310, 316, 334, 340, 857, 1133, 1340
EarI	CTCTTC	7 cut(s)	310, 316, 334, 340, 857, 1133, 1340
EciI	GGCGGA	4 cut(s)	59, 88, 283, 1547
EclXI	CGGCCG	1 cut(s)	571
Eco105I	TACGTA	1 cut(s)	2007
Eco130I	CCWWGG	3 cut(s)	918, 978, 1896
Eco147I	AGGCCT	1 cut(s)	1952
Eco24I	GRGCYC	2 cut(s)	894, 944
Eco47I	GGWCC	2 cut(s)	245, 922
Eco52I	CGGCCG	1 cut(s)	571
Eco88I	CYCGRG	2 cut(s)	190, 677
Eco91I	GGTNACC	1 cut(s)	719
EcoNI	CCTNNNNNAGG	2 cut(s)	680, 1441
EcoO65I	GGTNACC	1 cut(s)	719
EcoRI	GAATTC	1 cut(s)	985
EcoRII	CCWGG	6 cut(s)	19, 711, 897, 924, 1049, 1481
EcoT14I	CCWWGG	3 cut(s)	918, 978, 1896
EcoT38I	GRGCYC	2 cut(s)	894, 944
EgeI	GGCGCC	1 cut(s)	655
EheI	GGCGCC	1 cut(s)	655
ErhI	CCWWGG	3 cut(s)	918, 978, 1896
Esp3I	CGTCTC	1 cut(s)	187
FaqI	GGGAC	8 cut(s)	224, 231, 401, 433, 685, 745, 1111, 1878
FauI	CCCGC	2 cut(s)	710, 776
FbaI	TGATCA	3 cut(s)	750, 1420, 1599
FblI	GTMKAC	1 cut(s)	2009
FokI	GGATG	8 cut(s)	53, 115, 265, 539, 545, 1459, 1643, 1771
FriOI	GRGCYC	2 cut(s)	894, 944
FspBI	CTAG	2 cut(s)	1056, 1245
GlaI	GCGC	9 cut(s)	412, 457, 614, 655, 710, 1216, 1570, 1579, 2133
GsaI	CCCAGC	1 cut(s)	613
GsuI	CTGGAG	1 cut(s)	2082
HaeII	RGCGCY	2 cut(s)	616, 657
HaeIII	GGCC	8 cut(s)	91, 398, 573, 846, 917, 929, 1518, 1952
HapII	CCGG	3 cut(s)	885, 990, 1469
HgaI	GACGC	1 cut(s)	207
HhaI	GCGC	9 cut(s)	413, 458, 615, 656, 711, 1217, 1571, 1580, 2134
Hin1I	GRCGYC	2 cut(s)	199, 654
Hin6I	GCGC	9 cut(s)	411, 456, 613, 654, 709, 1215, 1569, 1578, 2132
HinP1I	GCGC	9 cut(s)	411, 456, 613, 654, 709, 1215, 1569, 1578, 2132
HincII	GTYRAC	3 cut(s)	739, 1100, 1708
HindII	GTYRAC	3 cut(s)	739, 1100, 1708
HinfI	GANTC	6 cut(s)	172, 290, 785, 821, 1115, 1461
HpaII	CCGG	3 cut(s)	885, 990, 1469
HphI	GGTGA	7 cut(s)	112, 247, 625, 757, 1471, 1648, 1940
Hpy166II	GTNNAC	6 cut(s)	739, 907, 1100, 1673, 1708, 2010
Hpy188III	TCNNGA	7 cut(s)	192, 679, 1154, 1387, 1418, 1597, 2099
Hpy8I	GTNNAC	6 cut(s)	739, 907, 1100, 1673, 1708, 2010
Hpy99I	CGWCG	3 cut(s)	201, 500, 1795
HpyAV	CCTTC	9 cut(s)	48, 268, 676, 920, 1028, 1082, 1084, 1932, 1969
HpyCH4III	ACNGT	5 cut(s)	719, 1298, 1415, 1477, 2127
HpyCH4IV	ACGT	1 cut(s)	2006
HpyF3I	CTNAG	5 cut(s)	973, 1455, 1665, 1947, 2156
HpySE526I	ACGT	1 cut(s)	2006
Hsp92I	GRCGYC	2 cut(s)	199, 654
HspAI	GCGC	9 cut(s)	411, 456, 613, 654, 709, 1215, 1569, 1578, 2132
KasI	GGCGCC	1 cut(s)	653
KroI	GCCGGC	1 cut(s)	884
KroNI	GCCGGC	1 cut(s)	886
Ksp22I	TGATCA	3 cut(s)	750, 1420, 1599
Kzo9I	GATC	4 cut(s)	750, 1420, 1599, 2159
LguI	GCTCTTC	3 cut(s)	857, 1133, 1340
LmnI	GCTCC	6 cut(s)	427, 513, 581, 731, 776, 1615
Lsp1109I	GCAGC	7 cut(s)	100, 119, 208, 1267, 1567, 1630, 2078
LweI	GCATC	5 cut(s)	1099, 1174, 1437, 1647, 1729
MaeI	CTAG	2 cut(s)	1056, 1245
MaeII	ACGT	1 cut(s)	2006
MaeIII	GTNAC	6 cut(s)	118, 719, 1477, 1687, 2002, 2121
MalI	GATC	4 cut(s)	752, 1422, 1601, 2161
MbiI	CCGCTC	4 cut(s)	576, 726, 771, 1143
MboI	GATC	4 cut(s)	750, 1420, 1599, 2159
MfeI	CAATTG	1 cut(s)	47
MflI	RGATCY	1 cut(s)	2159
MhlI	GDGCHC	5 cut(s)	664, 894, 909, 944, 1164
MlsI	TGGCCA	2 cut(s)	846, 917
MluI	ACGCGT	1 cut(s)	1854
MluNI	TGGCCA	2 cut(s)	846, 917
Mly113I	GGCGCC	1 cut(s)	654
MlyI	GAGTC	1 cut(s)	830
MmeI	TCCRAC	5 cut(s)	83, 389, 498, 753, 1434
Mox20I	TGGCCA	2 cut(s)	846, 917
MroNI	GCCGGC	1 cut(s)	884
MscI	TGGCCA	2 cut(s)	846, 917
MseI	TTAA	4 cut(s)	179, 1812, 1883, 1913
MslI	CAYNNNNRTG	1 cut(s)	81
Msp20I	TGGCCA	2 cut(s)	846, 917
MspA1I	CMGCKG	3 cut(s)	113, 394, 2063
MspI	CCGG	3 cut(s)	885, 990, 1469
MspR9I	CCNGG	7 cut(s)	21, 713, 899, 926, 1051, 1469, 1483
MunI	CAATTG	1 cut(s)	47
Mva1269I	GAATGC	1 cut(s)	1201
MvaI	CCWGG	6 cut(s)	21, 713, 899, 926, 1051, 1483
MvnI	CGCG	2 cut(s)	411, 1856
NaeI	GCCGGC	1 cut(s)	886
NarI	GGCGCC	1 cut(s)	654
NciI	CCSGG	1 cut(s)	1469
NcoI	CCATGG	1 cut(s)	918
NdeII	GATC	4 cut(s)	750, 1420, 1599, 2159
NgoMIV	GCCGGC	1 cut(s)	884
NheI	GCTAGC	1 cut(s)	1244
NlaIV	GGNNCC	4 cut(s)	247, 509, 655, 2058
NmeAIII	GCCGAG	1 cut(s)	549
NmuCI	GTSAC	2 cut(s)	118, 1477
NspI	RCATGY	1 cut(s)	709
PaeI	GCATGC	1 cut(s)	709
PaeR7I	CTCGAG	2 cut(s)	190, 677
PagI	TCATGA	2 cut(s)	1386, 1596
PceI	AGGCCT	1 cut(s)	1952
PciSI	GCTCTTC	3 cut(s)	857, 1133, 1340
PcsI	WCGNNNNNNNCGW	4 cut(s)	381, 576, 675, 1005
PctI	GAATGC	1 cut(s)	1201
PdiI	GCCGGC	1 cut(s)	886
PfeI	GAWTC	5 cut(s)	172, 290, 785, 1115, 1461
PflFI	GACNNNGTC	2 cut(s)	734, 1475
PleI	GAGTC	1 cut(s)	829
PluTI	GGCGCC	1 cut(s)	657
PpsI	GAGTC	1 cut(s)	829
Ppu21I	YACGTR	1 cut(s)	2007
PshAI	GACNNNNGTC	1 cut(s)	243
PshBI	ATTAAT	1 cut(s)	1812
Psp6I	CCWGG	6 cut(s)	19, 711, 897, 924, 1049, 1481
PspEI	GGTNACC	1 cut(s)	719
PspFI	CCCAGC	1 cut(s)	609
PspGI	CCWGG	6 cut(s)	19, 711, 897, 924, 1049, 1481
PspN4I	GGNNCC	4 cut(s)	247, 509, 655, 2058
PspPI	GGNCC	3 cut(s)	245, 397, 922
PstI	CTGCAG	1 cut(s)	1774
PstNI	CAGNNNCTG	1 cut(s)	113
PsuI	RGATCY	1 cut(s)	2159
PsyI	GACNNNGTC	2 cut(s)	734, 1475
PvuII	CAGCTG	1 cut(s)	394
RsaI	GTAC	2 cut(s)	1799, 1970
RsaNI	GTAC	2 cut(s)	1798, 1969
RseI	CAYNNNNRTG	1 cut(s)	81
SapI	GCTCTTC	3 cut(s)	857, 1133, 1340
SaqAI	TTAA	4 cut(s)	179, 1812, 1883, 1913
Sau3AI	GATC	4 cut(s)	750, 1420, 1599, 2159
Sau96I	GGNCC	3 cut(s)	245, 397, 922
SchI	GAGTC	1 cut(s)	830
ScrFI	CCNGG	7 cut(s)	21, 713, 899, 926, 1051, 1469, 1483
SduI	GDGCHC	5 cut(s)	664, 894, 909, 944, 1164
SfaNI	GCATC	5 cut(s)	1099, 1174, 1437, 1647, 1729
SfcI	CTRYAG	3 cut(s)	742, 808, 1770
SfoI	GGCGCC	1 cut(s)	655
Sfr274I	CTCGAG	2 cut(s)	190, 677
SinI	GGWCC	2 cut(s)	245, 922
SlaI	CTCGAG	2 cut(s)	190, 677
SmiMI	CAYNNNNRTG	1 cut(s)	81
SmlI	CTYRAG	4 cut(s)	190, 443, 677, 937
SmoI	CTYRAG	4 cut(s)	190, 443, 677, 937
SnaBI	TACGTA	1 cut(s)	2007
SphI	GCATGC	1 cut(s)	709
SseBI	AGGCCT	1 cut(s)	1952
SspDI	GGCGCC	1 cut(s)	653
SspMI	CTAG	2 cut(s)	1056, 1245
StuI	AGGCCT	1 cut(s)	1952
StyD4I	CCNGG	7 cut(s)	19, 711, 897, 924, 1049, 1467, 1481
StyI	CCWWGG	3 cut(s)	918, 978, 1896
TaaI	ACNGT	5 cut(s)	719, 1298, 1415, 1477, 2127
TaiI	ACGT	1 cut(s)	2009
TaqI	TCGA	4 cut(s)	26, 191, 375, 678
TaqII	GACCGA	1 cut(s)	233
TatI	WGTACW	2 cut(s)	1797, 1968
TauI	GCSGC	4 cut(s)	113, 204, 227, 576
TfiI	GAWTC	5 cut(s)	172, 290, 785, 1115, 1461
Tru1I	TTAA	4 cut(s)	179, 1812, 1883, 1913
Tru9I	TTAA	4 cut(s)	179, 1812, 1883, 1913
TscAI	CASTG	4 cut(s)	21, 937, 1357, 1854
TseFI	GTSAC	2 cut(s)	118, 1477
TseI	GCWGC	7 cut(s)	107, 113, 221, 1255, 1555, 1618, 2091
Tsp45I	GTSAC	2 cut(s)	118, 1477
TspDTI	ATGAA	9 cut(s)	117, 164, 1358, 1778, 1793, 1988, 2036, 2085, 2159
TspGWI	ACGGA	1 cut(s)	2024
TspRI	CASTG	4 cut(s)	21, 937, 1357, 1854
Tth111I	GACNNNGTC	2 cut(s)	734, 1475
VneI	GTGCAC	1 cut(s)	905
VpaK11BI	GGWCC	2 cut(s)	245, 922
VspI	ATTAAT	1 cut(s)	1812
XagI	CCTNNNNNAGG	2 cut(s)	680, 1441
XapI	RAATTY	2 cut(s)	985, 2045
XceI	RCATGY	1 cut(s)	709
XhoI	CTCGAG	2 cut(s)	190, 677
XmiI	GTMKAC	1 cut(s)	2009
XspI	CTAG	2 cut(s)	1056, 1245

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.