Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003659

Physical Location & Seq

Genomic Coordinates Reverse (-)

5317496 .. 5326928

Gene Structure 9433 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1046600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1674 bp

ATGGCATCGTGGTTCTCTCGCCTCCGCCGTTCCTCACAGCGCTTCTTCTTCACCTCCTCCGCCCTCCGAACCTCCATGTCCACCAAGCTGGCGAGAACCCAAAATACAATGAACTCCGAGAGCCCGAAAACCCCATTTCTCTTCTGGTCGAGCTCGCTGCTTCCGGTCGCACTCGCAGTCTCAGCTGGATCGCTTGCCCTCCGCCCCCAGTCGGACCCATCACTCTGTGAAGCCCCCAATTCAAATTCTCGCAACGAGTTTGTGGTTAAAGGGTTGCGCAAGGAAGTCCCACAAGAGCTTATTGATGAATTGAAGGATGTTTGTCAAGATAACATGACAATGGATTATGAGGAGAGGGATAACCATGGAAAACCGCAGAGCAGCTTTCACAGAGCGGTTAATATTCCCGACATCGTTGTGTTTCCGAGGTCTGAAGAGCAAGTTTCTAAGATAGTCAGGTCATGCGACAAGCATAAGGTTCCAATTGTACCATATGGCGGAGCTACATCCCTTGAGGGTCACACCTTGTCTCCCAATGGAGGTGTTTGCATTGACATGTCATTAATGAATCATGTTAAAGCATTACATATTGAGGACATGGATGTTGTTGTTGAGCCTGGGATTGGATGGATGGAGCTTAATGAATACCTGGCTCCTTATGGTCTATTTTTTCCCCTGGATCCAGGGCCTGGTGCAACAATTGGAGGAATGTGCGCGACACGTTGCTCTGGTTCCTTAGCTGTTAGGTATGGAACCATGCGTGATAATGTCATCAGTCTCAAGGTAGTTCTTGCAAATGGAGATATTGTTAAGACTGCATCTCGTGCTAGAAAGAGTGCTGCAGGGTATGATTTGACCCGCCTGGTGATTGGAAGTGAAGGAACACTAGGTGTAATAACGGAAGTCACTCTACGCCTTCAGAAAATTCCGCAATACTCAGTGGTTGCTATGTGTAATTTCCCTTCATTTAAGGATGCAGCAGATGTTGCTATTGCTACTATGCTGTCAGGCATTCAGGTTTCACGGGTTGAACTACTGGATGAGGTTCAAGTGAGGGCAATCAACATTGCGAATGGGAAGAATTTTCCTGAAACTCCAACTTTAATGTTCGAATTTATAGGCACAGAGGCATATTCTCATGAGCAAACCCATATAGTTCAACAGATTGTTTCTGAACACAATGGCTCAGACTTTGTTTTTGCGGAAGACCCTGAAGCTAAAAAGGAACTCTGGAAGATAAGGAAAGAGGCACTCTGGGCATGCTTTGCAATGGAGCCTAATTTTGAGGCAATGATTACGGATGTATGTGTTCCTTTATCATGTCTTGCAGAATTGATATCAAGGTCCAAAGAACAGCTGGATGCATCAGAACTTGTTTGCACAGTTATTGCTCATGCTGGTGACGGGAACTTCCACACATTTATTCTCTTTGACCCTAACAATGAAGAACATCGAAGAGAAGCAGAGAGACTGAGCCATTTTATGGTCCATGCTGCCTTGTCAATGGAAGGAACATGTACGGGTGAACATGGCGTTGGTACAGGAAAAATGAAGTATCTTGAGCAAGAACTGGGAATGGAGGCTTTGAAGACAATGAAAAGAATAAAAGCAGCATTAGATCCCAACAACATTATGAATCCAGGAAAGCTAATTCCTGCTCATGTTTGTTTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0003973 GO:0004457 GO:0004458 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006081 GO:0008144 GO:0008150 GO:0008152 GO:0008720 GO:0008891 GO:0009056 GO:0009438 GO:0009987 GO:0016491 GO:0016614 GO:0016616 GO:0016898 GO:0016899 GO:0017076 GO:0019154 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0042180 GO:0042182 GO:0042802 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044248 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046185 GO:0048037 GO:0050660 GO:0050662 GO:0051596 GO:0055114 GO:0071704 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00620 map00620

Pfam Domains

Protein Families

FAD-oxidase_C FAD_binding_4

Protein Analysis

558

Amino Acids

61.48

Weight (kDa)

5.79

Isoelectric Point (pI)

43.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_4	PF01565	136 - 272	5.7e-39	FAD binding domain
FAD-oxidase_C	PF02913	309 - 550	1.5e-73	FAD linked oxidases, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003659)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G06580
fragaria_vesca	FvH4_6g21120 FvH4_6g21120 FvH4_6g21120 FvH4_6g21120 FvH4_6g21120 FvH4_6g21120 FvH4_6g21120
malus_domestica	MD12G1046600.v1.1 MD12G1046700.v1.1 MD14G1045600.v1.1
prunus_persica	Prupe.7G088800_v2.0.a1 Prupe.7G088800_v2.0.a1 Prupe.7G088800_v2.0.a1 Prupe.7G088800_v2.0.a1 Prupe.7G088800_v2.0.a1 Prupe.7G088900_v2.0.a1 Prupe.7G088900_v2.0.a1 Prupe.7G088900_v2.0.a1 Prupe.7G088900_v2.0.a1
pyrus_communis	pycom12g04020 pycom12g04030
rosa_chinensis	RchiOBHm_Chr3g0477531
rosa_laevigata	RLG00000023711
rosa_multiflora	Rmu_co8386717.1_g000001 Rmu_sc0000775.1_g000016
rosa_roxburghii	Rroxscaffold_6G00403860 Rroxscaffold_6G00403870 Rroxscaffold_6G00404560
rosa_rugosa	Rorug03G0161800
rosa_samantha	Rh3AG211800 Rh3BG245600 Rh3CG239200 Rh3DG238700
rosa_wichuraiana	Rw3G019190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	278
AccB7I	CCANNNNNTGG	2 cut(s)	689, 1483
AccBSI	CCGCTC	1 cut(s)	395
AccII	CGCG	1 cut(s)	716
AciI	CCGC	9 cut(s)	25, 60, 202, 374, 395, 498, 859, 929, 1202
AclWI	GGATC	4 cut(s)	196, 674, 687, 1613
AcsI	RAATTY	4 cut(s)	244, 924, 1081, 1112
AcuI	CTGAAG	3 cut(s)	453, 902, 1233
AdeI	CACNNNGTG	2 cut(s)	227, 890
AfaI	GTAC	3 cut(s)	489, 1519, 1540
AfeI	AGCGCT	1 cut(s)	41
AfiI	CCNNNNNNNGG	6 cut(s)	211, 497, 539, 623, 689, 1483
AflIII	ACRYGT	3 cut(s)	555, 719, 1514
AgsI	TTSAA	6 cut(s)	243, 313, 1031, 1049, 1160, 1588
AjnI	CCWGG	7 cut(s)	616, 648, 675, 682, 688, 861, 1639
AjuI	GAANNNNNNNTTGG	2 cut(s)	1518, 1550
Alw21I	GWGCWC	1 cut(s)	155
Alw26I	GTCTC	4 cut(s)	184, 534, 782, 1462
AlwI	GGATC	4 cut(s)	196, 674, 687, 1613
AlwNI	CAGNNNCTG	1 cut(s)	689
Aor51HI	AGCGCT	1 cut(s)	41
AoxI	GGCC	1 cut(s)	686
ApeKI	GCWGC	6 cut(s)	157, 381, 839, 977, 1493, 1610
ApoI	RAATTY	4 cut(s)	244, 924, 1081, 1112
ArsI	GACNNNNNNTTYG	2 cut(s)	1181, 1213
AseI	ATTAAT	1 cut(s)	563
AspLEI	GCGC	3 cut(s)	42, 279, 716
AspS9I	GGNCC	4 cut(s)	214, 686, 1344, 1486
AsuHPI	GGTGA	4 cut(s)	43, 877, 1412, 1535
AsuII	TTCGAA	1 cut(s)	1110
AvaII	GGWCC	3 cut(s)	214, 1344, 1486
BamHI	GGATCC	1 cut(s)	679
BanII	GRGCYC	2 cut(s)	125, 155
BarI	GAAGNNNNNNTAC	4 cut(s)	894, 926, 946, 978
BauI	CACGAG	1 cut(s)	822
BbsI	GAAGAC	2 cut(s)	1212, 1595
Bbv12I	GWGCWC	1 cut(s)	155
BbvI	GCAGC	6 cut(s)	144, 393, 826, 989, 1480, 1622
BccI	CCATC	3 cut(s)	226, 621, 625
BceAI	ACGGC	1 cut(s)	12
BcgI	CGANNNNNNTGC	2 cut(s)	139, 173
BciT130I	CCWGG	7 cut(s)	618, 650, 677, 684, 690, 863, 1641
BcoDI	GTCTC	4 cut(s)	184, 534, 782, 1462
BfaI	CTAG	2 cut(s)	828, 887
BfmI	CTRYAG	1 cut(s)	840
BfoI	RGCGCY	1 cut(s)	43
BisI	GCNGC	6 cut(s)	158, 382, 840, 978, 1494, 1611
BlsI	GCNGC	6 cut(s)	159, 383, 841, 979, 1495, 1612
Bme1390I	CCNGG	7 cut(s)	618, 650, 677, 684, 690, 863, 1641
Bme18I	GGWCC	3 cut(s)	214, 1344, 1486
BmgT120I	GGNCC	4 cut(s)	214, 686, 1344, 1486
BmiI	GGNNCC	7 cut(s)	216, 480, 654, 681, 733, 754, 1275
BmrFI	CCNGG	7 cut(s)	618, 650, 677, 684, 690, 863, 1641
BmrI	ACTGGG	2 cut(s)	202, 1580
BmsI	GCATC	5 cut(s)	14, 827, 964, 1351, 1373
BmuI	ACTGGG	2 cut(s)	202, 1580
BpiI	GAAGAC	2 cut(s)	1212, 1595
Bpu10I	CCTNAGC	1 cut(s)	736
Bpu14I	TTCGAA	1 cut(s)	1110
BpuEI	CTTGAG	3 cut(s)	533, 764, 1580
BsaJI	CCNNGG	5 cut(s)	364, 425, 617, 675, 683
BsaWI	WCCGGW	1 cut(s)	163
BsaXI	ACNNNNNCTCC	4 cut(s)	514, 544, 792, 822
Bsc4I	CCNNNNNNNGG	6 cut(s)	211, 497, 539, 623, 689, 1483
Bse1I	ACTGG	3 cut(s)	208, 1041, 1575
Bse3DI	GCAATG	3 cut(s)	1065, 1275, 1296
BseBI	CCWGG	7 cut(s)	618, 650, 677, 684, 690, 863, 1641
BseDI	CCNNGG	5 cut(s)	364, 425, 617, 675, 683
BseGI	GGATG	9 cut(s)	322, 506, 607, 632, 636, 979, 1045, 1306, 1366
BseLI	CCNNNNNNNGG	6 cut(s)	211, 497, 539, 623, 689, 1483
BseMI	GCAATG	3 cut(s)	1065, 1275, 1296
BseMII	CTCAG	4 cut(s)	195, 951, 1200, 1463
BseNI	ACTGG	3 cut(s)	208, 1041, 1575
BseRI	GAGGAG	2 cut(s)	46, 365
BseXI	GCAGC	6 cut(s)	144, 393, 826, 989, 1480, 1622
Bsh1236I	CGCG	1 cut(s)	716
Bsh1285I	CGRYCG	1 cut(s)	168
BshFI	GGCC	1 cut(s)	688
BsiEI	CGRYCG	1 cut(s)	168
BsiHKAI	GWGCWC	1 cut(s)	155
BsiSI	CCGG	1 cut(s)	164
BslFI	GGGAC	1 cut(s)	272
BslI	CCNNNNNNNGG	6 cut(s)	211, 497, 539, 623, 689, 1483
BsmAI	GTCTC	4 cut(s)	184, 534, 782, 1462
BsmFI	GGGAC	1 cut(s)	272
BsmI	GAATGC	1 cut(s)	1011
BsnI	GGCC	1 cut(s)	688
Bsp119I	TTCGAA	1 cut(s)	1110
Bsp1286I	GDGCHC	2 cut(s)	125, 155
Bsp143I	GATC	3 cut(s)	188, 679, 1618
Bsp19I	CCATGG	1 cut(s)	364
BspACI	CCGC	9 cut(s)	25, 60, 202, 374, 395, 498, 859, 929, 1202
BspANI	GGCC	1 cut(s)	688
BspCNI	CTCAG	4 cut(s)	194, 950, 1199, 1464
BspFNI	CGCG	1 cut(s)	716
BspHI	TCATGA	1 cut(s)	1138
BspLI	GGNNCC	7 cut(s)	216, 480, 654, 681, 733, 754, 1275
BspMAI	CTGCAG	1 cut(s)	844
BspPI	GGATC	4 cut(s)	196, 674, 687, 1613
BspQI	GCTCTTC	1 cut(s)	429
BspT104I	TTCGAA	1 cut(s)	1110
BsrBI	CCGCTC	1 cut(s)	395
BsrDI	GCAATG	3 cut(s)	1065, 1275, 1296
BsrI	ACTGG	3 cut(s)	208, 1041, 1575
BssECI	CCNNGG	5 cut(s)	364, 425, 617, 675, 683
BssMI	GATC	3 cut(s)	188, 679, 1618
BssSI	CACGAG	1 cut(s)	822
BssT1I	CCWWGG	1 cut(s)	364
Bst2BI	CACGAG	1 cut(s)	822
Bst2UI	CCWGG	7 cut(s)	618, 650, 677, 684, 690, 863, 1641
Bst4CI	ACNGT	1 cut(s)	1384
Bst6I	CTCTTC	3 cut(s)	146, 429, 1450
BstAPI	GCANNNNNTGC	3 cut(s)	824, 986, 1265
BstBI	TTCGAA	1 cut(s)	1110
BstC8I	GCNNGC	4 cut(s)	90, 155, 195, 1261
BstDEI	CTNAG	6 cut(s)	181, 447, 736, 937, 1186, 1472
BstDSI	CCRYGG	1 cut(s)	364
BstF5I	GGATG	9 cut(s)	322, 506, 607, 632, 636, 979, 1045, 1306, 1366
BstFNI	CGCG	1 cut(s)	716
BstH2I	RGCGCY	1 cut(s)	43
BstHHI	GCGC	3 cut(s)	42, 279, 716
BstKTI	GATC	3 cut(s)	191, 682, 1621
BstMAI	GTCTC	4 cut(s)	184, 534, 782, 1462
BstMBI	GATC	3 cut(s)	188, 679, 1618
BstMCI	CGRYCG	1 cut(s)	168
BstMWI	GCNNNNNNNGC	5 cut(s)	182, 824, 986, 1256, 1265
BstNI	CCWGG	7 cut(s)	618, 650, 677, 684, 690, 863, 1641
BstNSI	RCATGY	3 cut(s)	559, 1263, 1518
BstSCI	CCNGG	7 cut(s)	616, 648, 675, 682, 688, 861, 1639
BstSFI	CTRYAG	1 cut(s)	840
BstUI	CGCG	1 cut(s)	716
BstV1I	GCAGC	6 cut(s)	144, 393, 826, 989, 1480, 1622
BstV2I	GAAGAC	2 cut(s)	1212, 1595
BstX2I	RGATCY	2 cut(s)	679, 1618
BstXI	CCANNNNNNTGG	1 cut(s)	88
BstYI	RGATCY	2 cut(s)	679, 1618
BsuRI	GGCC	1 cut(s)	688
BtgI	CCRYGG	1 cut(s)	364
BtsCI	GGATG	9 cut(s)	322, 506, 607, 632, 636, 979, 1045, 1306, 1366
BtsIMutI	CAGTG	1 cut(s)	945
Cac8I	GCNNGC	4 cut(s)	90, 155, 195, 1261
CaiI	CAGNNNCTG	1 cut(s)	689
CciI	TCATGA	1 cut(s)	1138
CfoI	GCGC	3 cut(s)	42, 279, 716
Cfr13I	GGNCC	4 cut(s)	214, 686, 1344, 1486
Csp6I	GTAC	3 cut(s)	488, 1518, 1539
CviQI	GTAC	3 cut(s)	488, 1518, 1539
DdeI	CTNAG	6 cut(s)	181, 447, 736, 937, 1186, 1472
DpnI	GATC	3 cut(s)	190, 681, 1620
DpnII	GATC	3 cut(s)	188, 679, 1618
DraIII	CACNNNGTG	2 cut(s)	227, 890
Eam1104I	CTCTTC	3 cut(s)	146, 429, 1450
EarI	CTCTTC	3 cut(s)	146, 429, 1450
EciI	GGCGGA	4 cut(s)	14, 49, 191, 513
Ecl136II	GAGCTC	1 cut(s)	153
Eco130I	CCWWGG	1 cut(s)	364
Eco24I	GRGCYC	2 cut(s)	125, 155
Eco32I	GATATC	1 cut(s)	1338
Eco47I	GGWCC	3 cut(s)	214, 1344, 1486
Eco47III	AGCGCT	1 cut(s)	41
Eco53kI	GAGCTC	1 cut(s)	153
Eco57I	CTGAAG	3 cut(s)	453, 902, 1233
EcoICRI	GAGCTC	1 cut(s)	153
EcoO109I	RGGNCCY	1 cut(s)	686
EcoRII	CCWGG	7 cut(s)	616, 648, 675, 682, 688, 861, 1639
EcoRV	GATATC	1 cut(s)	1338
EcoT14I	CCWWGG	1 cut(s)	364
EcoT22I	ATGCAT	1 cut(s)	1366
EcoT38I	GRGCYC	2 cut(s)	125, 155
ErhI	CCWWGG	1 cut(s)	364
FaqI	GGGAC	1 cut(s)	272
FauI	CCCGC	1 cut(s)	866
FauNDI	CATATG	1 cut(s)	493
Fnu4HI	GCNGC	6 cut(s)	158, 382, 840, 978, 1494, 1611
FokI	GGATG	9 cut(s)	329, 493, 614, 639, 643, 986, 1052, 1313, 1373
FriOI	GRGCYC	2 cut(s)	125, 155
Fsp4HI	GCNGC	6 cut(s)	158, 382, 840, 978, 1494, 1611
FspBI	CTAG	2 cut(s)	828, 887
FspI	TGCGCA	1 cut(s)	278
GlaI	GCGC	3 cut(s)	41, 278, 715
GluI	GCNGC	6 cut(s)	158, 382, 840, 978, 1494, 1611
HaeII	RGCGCY	1 cut(s)	43
HaeIII	GGCC	1 cut(s)	688
HapII	CCGG	1 cut(s)	164
HhaI	GCGC	3 cut(s)	42, 279, 716
Hin6I	GCGC	3 cut(s)	40, 277, 714
HinP1I	GCGC	3 cut(s)	40, 277, 714
HinfI	GANTC	2 cut(s)	568, 1636
HpaII	CCGG	1 cut(s)	164
HphI	GGTGA	4 cut(s)	43, 877, 1412, 1535
Hpy166II	GTNNAC	2 cut(s)	81, 1526
Hpy188I	TCNGA	9 cut(s)	68, 118, 214, 426, 433, 921, 1174, 1189, 1369
Hpy188III	TCNNGA	6 cut(s)	326, 407, 1088, 1139, 1231, 1559
Hpy8I	GTNNAC	2 cut(s)	81, 1526
HpyAV	CCTTC	5 cut(s)	307, 872, 926, 972, 1502
HpyCH4III	ACNGT	1 cut(s)	1384
HpyCH4IV	ACGT	1 cut(s)	721
HpyF10VI	GCNNNNNNNGC	5 cut(s)	182, 824, 986, 1256, 1265
HpyF3I	CTNAG	6 cut(s)	181, 447, 736, 937, 1186, 1472
HpySE526I	ACGT	1 cut(s)	721
HspAI	GCGC	3 cut(s)	40, 277, 714
Kzo9I	GATC	3 cut(s)	188, 679, 1618
LguI	GCTCTTC	1 cut(s)	429
LmnI	GCTCC	4 cut(s)	500, 634, 658, 1273
Lsp1109I	GCAGC	6 cut(s)	144, 393, 826, 989, 1480, 1622
LweI	GCATC	5 cut(s)	14, 827, 964, 1351, 1373
MaeI	CTAG	2 cut(s)	828, 887
MaeII	ACGT	1 cut(s)	721
MaeIII	GTNAC	3 cut(s)	518, 904, 1400
MalI	GATC	3 cut(s)	190, 681, 1620
MbiI	CCGCTC	1 cut(s)	395
MboI	GATC	3 cut(s)	188, 679, 1618
MfeI	CAATTG	2 cut(s)	483, 699
MflI	RGATCY	2 cut(s)	679, 1618
MhlI	GDGCHC	2 cut(s)	125, 155
MmeI	TCCRAC	2 cut(s)	192, 1121
Mph1103I	ATGCAT	1 cut(s)	1366
MseI	TTAA	9 cut(s)	267, 399, 563, 576, 639, 810, 969, 1103, 1672
MslI	CAYNNNNRTG	4 cut(s)	338, 416, 554, 1398
MspA1I	CMGCKG	2 cut(s)	185, 1357
MspI	CCGG	1 cut(s)	164
MspR9I	CCNGG	7 cut(s)	618, 650, 677, 684, 690, 863, 1641
MunI	CAATTG	2 cut(s)	483, 699
Mva1269I	GAATGC	1 cut(s)	1011
MvaI	CCWGG	7 cut(s)	618, 650, 677, 684, 690, 863, 1641
MvnI	CGCG	1 cut(s)	716
MwoI	GCNNNNNNNGC	5 cut(s)	182, 824, 986, 1256, 1265
NcoI	CCATGG	1 cut(s)	364
NdeI	CATATG	1 cut(s)	493
NdeII	GATC	3 cut(s)	188, 679, 1618
NlaIV	GGNNCC	7 cut(s)	216, 480, 654, 681, 733, 754, 1275
NmuCI	GTSAC	3 cut(s)	518, 904, 1400
NsbI	TGCGCA	1 cut(s)	278
NsiI	ATGCAT	1 cut(s)	1366
NspI	RCATGY	3 cut(s)	559, 1263, 1518
NspV	TTCGAA	1 cut(s)	1110
PaeI	GCATGC	1 cut(s)	1263
PagI	TCATGA	1 cut(s)	1138
PciI	ACATGT	2 cut(s)	555, 1514
PciSI	GCTCTTC	1 cut(s)	429
PcsI	WCGNNNNNNNCGW	1 cut(s)	25
PctI	GAATGC	1 cut(s)	1011
PfeI	GAWTC	2 cut(s)	568, 1636
PflMI	CCANNNNNTGG	2 cut(s)	689, 1483
PfoI	TCCNGGA	1 cut(s)	1639
PkrI	GCNGC	6 cut(s)	159, 383, 841, 979, 1495, 1612
PscI	ACATGT	2 cut(s)	555, 1514
PshBI	ATTAAT	1 cut(s)	563
Psp124BI	GAGCTC	1 cut(s)	155
Psp6I	CCWGG	7 cut(s)	616, 648, 675, 682, 688, 861, 1639
PspGI	CCWGG	7 cut(s)	616, 648, 675, 682, 688, 861, 1639
PspN4I	GGNNCC	7 cut(s)	216, 480, 654, 681, 733, 754, 1275
PspPI	GGNCC	4 cut(s)	214, 686, 1344, 1486
PsrI	GAACNNNNNNTAC	2 cut(s)	88, 120
PstI	CTGCAG	1 cut(s)	844
PstNI	CAGNNNCTG	1 cut(s)	689
PsuI	RGATCY	2 cut(s)	679, 1618
PvuII	CAGCTG	2 cut(s)	185, 1357
RsaI	GTAC	3 cut(s)	489, 1519, 1540
RsaNI	GTAC	3 cut(s)	488, 1518, 1539
RseI	CAYNNNNRTG	4 cut(s)	338, 416, 554, 1398
SacI	GAGCTC	1 cut(s)	155
SapI	GCTCTTC	1 cut(s)	429
SaqAI	TTAA	9 cut(s)	267, 399, 563, 576, 639, 810, 969, 1103, 1672
SatI	GCNGC	6 cut(s)	158, 382, 840, 978, 1494, 1611
Sau3AI	GATC	3 cut(s)	188, 679, 1618
Sau96I	GGNCC	4 cut(s)	214, 686, 1344, 1486
ScrFI	CCNGG	7 cut(s)	618, 650, 677, 684, 690, 863, 1641
SduI	GDGCHC	2 cut(s)	125, 155
SfaNI	GCATC	5 cut(s)	14, 827, 964, 1351, 1373
SfcI	CTRYAG	1 cut(s)	840
SfuI	TTCGAA	1 cut(s)	1110
SinI	GGWCC	3 cut(s)	214, 1344, 1486
SmiMI	CAYNNNNRTG	4 cut(s)	338, 416, 554, 1398
SmlI	CTYRAG	3 cut(s)	512, 779, 1559
SmoI	CTYRAG	3 cut(s)	512, 779, 1559
SphI	GCATGC	1 cut(s)	1263
SsiI	CCGC	9 cut(s)	25, 60, 202, 374, 395, 498, 859, 929, 1202
SspI	AATATT	1 cut(s)	403
SspMI	CTAG	2 cut(s)	828, 887
SstI	GAGCTC	1 cut(s)	155
StyD4I	CCNGG	7 cut(s)	616, 648, 675, 682, 688, 861, 1639
StyI	CCWWGG	1 cut(s)	364
TaaI	ACNGT	1 cut(s)	1384
TaiI	ACGT	1 cut(s)	724
TaqI	TCGA	3 cut(s)	149, 1110, 1453
TfiI	GAWTC	2 cut(s)	568, 1636
Tru1I	TTAA	9 cut(s)	267, 399, 563, 576, 639, 810, 969, 1103, 1672
Tru9I	TTAA	9 cut(s)	267, 399, 563, 576, 639, 810, 969, 1103, 1672
TscAI	CASTG	1 cut(s)	945
TseFI	GTSAC	3 cut(s)	518, 904, 1400
TseI	GCWGC	6 cut(s)	157, 381, 839, 977, 1493, 1610
Tsp45I	GTSAC	3 cut(s)	518, 904, 1400
TspDTI	ATGAA	9 cut(s)	125, 321, 581, 657, 954, 1458, 1565, 1610, 1649
TspGWI	ACGGA	2 cut(s)	914, 1313
TspRI	CASTG	1 cut(s)	945
Van91I	CCANNNNNTGG	2 cut(s)	689, 1483
VpaK11BI	GGWCC	3 cut(s)	214, 1344, 1486
VspI	ATTAAT	1 cut(s)	563
XapI	RAATTY	4 cut(s)	244, 924, 1081, 1112
XceI	RCATGY	3 cut(s)	559, 1263, 1518
XcmI	CCANNNNNNNNNTGG	2 cut(s)	141, 1354
XspI	CTAG	2 cut(s)	828, 887
Zsp2I	ATGCAT	1 cut(s)	1366

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.