Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003659

Physical Location & Seq

Genomic Coordinates Reverse (-)

5344241 .. 5363320

Gene Structure 19080 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1046700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1680 bp

ATGGCCATGGCGTCGTGGTTCTCTCGCCTCCGCCGTTCCTCACAGCGCTTCTTCTTCACCTCCTCCGCCCTCCGAACCTCCATGTCCACCAAGCTGGCGAGAACCCAAAATACAACGAACTCCGAGAGCCCGAAAACCCCATTTCTCTTGTGGTCGAGCTCGCTGCTTCCGGTCGCACTCGCAGTCTCAGCCGGATCGCTTGCCCTCCGCCCCCAATTGGACCCATCACTCTGTGAAGCCCCCAGTGCAAATTCTCGCAACGAGTTTGTTGTTAAAGGGCTGTGCAAGGAAGTCCCACAAGAGCTTGTTGATGAATTGAAGGATGTTTGTCAAGATAACATGACAATGGATTATGAGGAGAGGTATAACCATGGAAAACCGCAGAACAGCTTTCACAAAGCGGTTAATATTCCCGACATCGTTGTGTTTCCGGGGTCTGAAGAGCAAGTTTCTATGATAGTCAAGTCATGCGACAAGCATAAGGTTCCAATTGTACCATATGGCGGAGCTACATCCATTGAGGGTCACACCTTGTCTCCCAATGGAGGTGTTTGCATTGACATGTCATTAATGAATCATGTTAAAGCATTACATATTGAGGACATGGATGTTGTTGTTGAGCCTGGGATTGGATGGATGGAGCTTAATGAATACCTGGCTCCTTATGGTCTATTTTTTCCCCTGGATCCAGGGCCTGGTGCAACAATTGGAGGAATGTGCGCGACACGTTGCTCTGGTTCCTTAGCTGTTAGGTATGGAACCATGCGTGATAATGTCATCAGTCTCAAGGTAGTTCTTGCAAATGGAGATATTGTTAAGACTGCATCTCGTGCTAGAAAGAGTGCTGCAGGGTATGATTTGACCCGCCTGGTGATTGGAAGTGAAGGAACACTTGGTGTAATAACGGAAGTCACTCTACGCCTTCAGAAAATTCCGCAATACTCAGTGGTTGCTATGTGTAATTTCCCTTCAATTAAGGATGCAGCAGATGTTGCTATTGCTACTATGCTGTCGGGCATTCAGGTTTCACGGGTTGAACTACTGGATGAGGTTCAAGTGAGGGCAATCAACATTGCGAATGGGAAGAATTTTCTTGAAACTCCAACTTTAATGTTCGAATTTATAGGCACAGAGGCATATTCCCGTGAGCAAACCCATATAGTTCAACAGATTGTTTCTGAACACAATGGCTCAGACTTTGTTTTTGCGGAAGACCCTGAAGCTAAAAAGGAACTCTGGAAGATAAGGAAGGAGGCACTCTGGGCATGCTTTGCAATGGAGCCTAATTTTGAGGCAATGATTTCGGATGTATGTGTTCCTTTATCATGTCTTGCAGAATTGATATCAAGGTCCAAAGAACAGCTGGATGCATCAGAACTTGTTTGCACAGTTATTGCTCATGCTGGTGACGGGAACTTCCATACAGTGATTCTATTTGACCCTAACAATGAAGAACATCGACGAGAAGCAGAGAGACTGAACCATTTTATGGTCCATACTGCCTTGTCAATGGAAGGAACATGTACCGGTGAACATGGCGTTGGTACAGGAAAAATGAAGTATCTTGAGCAAGAACTGGGAATGGAGGCTTTGAAGACAATGAAAAGAATAAAAGCAGCATTAGATCCCAACAACACTATGAATCCAGGAAAGCTAATTCCTGCTCATGTTTGTTTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0003973 GO:0004457 GO:0004458 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006081 GO:0008144 GO:0008150 GO:0008152 GO:0008720 GO:0008891 GO:0009056 GO:0009438 GO:0009987 GO:0016491 GO:0016614 GO:0016616 GO:0016898 GO:0016899 GO:0017076 GO:0019154 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0042180 GO:0042182 GO:0042802 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044248 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046185 GO:0048037 GO:0050660 GO:0050662 GO:0051596 GO:0055114 GO:0071704 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00620 map00620

Pfam Domains

Protein Families

FAD-oxidase_C FAD_binding_4

Protein Analysis

560

Amino Acids

61.44

Weight (kDa)

5.67

Isoelectric Point (pI)

40.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_4	PF01565	138 - 274	1.4e-37	FAD binding domain
FAD-oxidase_C	PF02913	311 - 552	2e-70	FAD linked oxidases, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003659)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G06580
fragaria_vesca	FvH4_6g21120 FvH4_6g21120 FvH4_6g21120 FvH4_6g21120 FvH4_6g21120 FvH4_6g21120 FvH4_6g21120
malus_domestica	MD12G1046600.v1.1 MD12G1046700.v1.1 MD14G1045600.v1.1
prunus_persica	Prupe.7G088800_v2.0.a1 Prupe.7G088800_v2.0.a1 Prupe.7G088800_v2.0.a1 Prupe.7G088800_v2.0.a1 Prupe.7G088800_v2.0.a1 Prupe.7G088900_v2.0.a1 Prupe.7G088900_v2.0.a1 Prupe.7G088900_v2.0.a1 Prupe.7G088900_v2.0.a1
pyrus_communis	pycom12g04020 pycom12g04030
rosa_chinensis	RchiOBHm_Chr3g0477531
rosa_laevigata	RLG00000023711
rosa_multiflora	Rmu_co8386717.1_g000001 Rmu_sc0000775.1_g000016
rosa_roxburghii	Rroxscaffold_6G00403860 Rroxscaffold_6G00403870 Rroxscaffold_6G00404560
rosa_rugosa	Rorug03G0161800
rosa_samantha	Rh3AG211800 Rh3BG245600 Rh3CG239200 Rh3DG238700
rosa_wichuraiana	Rw3G019190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	695, 1489
AccII	CGCG	1 cut(s)	722
AciI	CCGC	9 cut(s)	31, 66, 208, 380, 401, 504, 865, 935, 1208
AclWI	GGATC	4 cut(s)	202, 680, 693, 1619
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	4 cut(s)	250, 930, 1087, 1118
AcuI	CTGAAG	3 cut(s)	459, 908, 1239
AcyI	GRCGYC	1 cut(s)	11
AdeI	CACNNNGTG	2 cut(s)	233, 896
AfaI	GTAC	3 cut(s)	495, 1525, 1546
AfeI	AGCGCT	1 cut(s)	47
AfiI	CCNNNNNNNGG	6 cut(s)	217, 503, 545, 629, 695, 1489
AflIII	ACRYGT	3 cut(s)	561, 725, 1520
AgeI	ACCGGT	1 cut(s)	1526
AgsI	TTSAA	7 cut(s)	319, 972, 1037, 1055, 1097, 1166, 1594
AjnI	CCWGG	7 cut(s)	622, 654, 681, 688, 694, 867, 1645
AjuI	GAANNNNNNNTTGG	4 cut(s)	876, 908, 1524, 1556
Alw21I	GWGCWC	1 cut(s)	161
Alw26I	GTCTC	4 cut(s)	190, 540, 788, 1468
AlwI	GGATC	4 cut(s)	202, 680, 693, 1619
AlwNI	CAGNNNCTG	1 cut(s)	695
Aor51HI	AGCGCT	1 cut(s)	47
AoxI	GGCC	2 cut(s)	3, 692
ApeKI	GCWGC	4 cut(s)	163, 845, 983, 1616
ApoI	RAATTY	4 cut(s)	250, 930, 1087, 1118
ArsI	GACNNNNNNTTYG	2 cut(s)	1187, 1219
AseI	ATTAAT	1 cut(s)	569
AsiGI	ACCGGT	1 cut(s)	1526
AspLEI	GCGC	2 cut(s)	48, 722
AspS9I	GGNCC	4 cut(s)	220, 692, 1350, 1492
AsuC2I	CCSGG	1 cut(s)	432
AsuHPI	GGTGA	4 cut(s)	49, 883, 1418, 1541
AsuII	TTCGAA	1 cut(s)	1116
AvaII	GGWCC	3 cut(s)	220, 1350, 1492
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	1 cut(s)	685
BanII	GRGCYC	2 cut(s)	131, 161
BarI	GAAGNNNNNNTAC	4 cut(s)	900, 932, 952, 984
BauI	CACGAG	1 cut(s)	828
BbsI	GAAGAC	2 cut(s)	1218, 1601
Bbv12I	GWGCWC	1 cut(s)	161
BbvI	GCAGC	4 cut(s)	150, 832, 995, 1628
BccI	CCATC	3 cut(s)	232, 627, 631
BceAI	ACGGC	1 cut(s)	18
BcgI	CGANNNNNNTGC	4 cut(s)	145, 179, 1284, 1318
BciT130I	CCWGG	7 cut(s)	624, 656, 683, 690, 696, 869, 1647
BcnI	CCSGG	1 cut(s)	432
BcoDI	GTCTC	4 cut(s)	190, 540, 788, 1468
BfaI	CTAG	1 cut(s)	834
BfmI	CTRYAG	1 cut(s)	846
BfoI	RGCGCY	1 cut(s)	49
BisI	GCNGC	4 cut(s)	164, 846, 984, 1617
BlsI	GCNGC	4 cut(s)	165, 847, 985, 1618
Bme1390I	CCNGG	8 cut(s)	432, 624, 656, 683, 690, 696, 869, 1647
Bme18I	GGWCC	3 cut(s)	220, 1350, 1492
BmgT120I	GGNCC	4 cut(s)	220, 692, 1350, 1492
BmiI	GGNNCC	7 cut(s)	222, 486, 660, 687, 739, 760, 1281
BmrFI	CCNGG	8 cut(s)	432, 624, 656, 683, 690, 696, 869, 1647
BmrI	ACTGGG	2 cut(s)	237, 1586
BmsI	GCATC	4 cut(s)	833, 970, 1357, 1379
BmuI	ACTGGG	2 cut(s)	237, 1586
BpiI	GAAGAC	2 cut(s)	1218, 1601
Bpu10I	CCTNAGC	1 cut(s)	742
Bpu14I	TTCGAA	1 cut(s)	1116
BpuEI	CTTGAG	2 cut(s)	770, 1586
BpuMI	CCSGG	1 cut(s)	432
BsaHI	GRCGYC	1 cut(s)	11
BsaJI	CCNNGG	6 cut(s)	6, 370, 431, 623, 681, 689
BsaWI	WCCGGW	2 cut(s)	169, 1526
BsaXI	ACNNNNNCTCC	4 cut(s)	520, 550, 798, 828
Bsc4I	CCNNNNNNNGG	6 cut(s)	217, 503, 545, 629, 695, 1489
Bse118I	RCCGGY	1 cut(s)	1526
Bse1I	ACTGG	3 cut(s)	243, 1047, 1581
Bse3DI	GCAATG	3 cut(s)	1071, 1281, 1302
BseBI	CCWGG	7 cut(s)	624, 656, 683, 690, 696, 869, 1647
BseDI	CCNNGG	6 cut(s)	6, 370, 431, 623, 681, 689
BseGI	GGATG	9 cut(s)	328, 512, 613, 638, 642, 985, 1051, 1312, 1372
BseLI	CCNNNNNNNGG	6 cut(s)	217, 503, 545, 629, 695, 1489
BseMI	GCAATG	3 cut(s)	1071, 1281, 1302
BseMII	CTCAG	3 cut(s)	201, 957, 1206
BseNI	ACTGG	3 cut(s)	243, 1047, 1581
BseRI	GAGGAG	2 cut(s)	52, 371
BseXI	GCAGC	4 cut(s)	150, 832, 995, 1628
Bsh1236I	CGCG	1 cut(s)	722
Bsh1285I	CGRYCG	1 cut(s)	174
BshFI	GGCC	2 cut(s)	5, 694
BshTI	ACCGGT	1 cut(s)	1526
BsiEI	CGRYCG	1 cut(s)	174
BsiHKAI	GWGCWC	1 cut(s)	161
BsiSI	CCGG	4 cut(s)	170, 192, 431, 1527
BslFI	GGGAC	1 cut(s)	278
BslI	CCNNNNNNNGG	6 cut(s)	217, 503, 545, 629, 695, 1489
BsmAI	GTCTC	4 cut(s)	190, 540, 788, 1468
BsmFI	GGGAC	1 cut(s)	278
BsmI	GAATGC	1 cut(s)	1017
BsnI	GGCC	2 cut(s)	5, 694
Bsp119I	TTCGAA	1 cut(s)	1116
Bsp1286I	GDGCHC	2 cut(s)	131, 161
Bsp143I	GATC	3 cut(s)	194, 685, 1624
Bsp19I	CCATGG	2 cut(s)	6, 370
BspACI	CCGC	9 cut(s)	31, 66, 208, 380, 401, 504, 865, 935, 1208
BspANI	GGCC	2 cut(s)	5, 694
BspCNI	CTCAG	3 cut(s)	200, 956, 1205
BspFNI	CGCG	1 cut(s)	722
BspLI	GGNNCC	7 cut(s)	222, 486, 660, 687, 739, 760, 1281
BspMAI	CTGCAG	1 cut(s)	850
BspPI	GGATC	4 cut(s)	202, 680, 693, 1619
BspQI	GCTCTTC	1 cut(s)	435
BspT104I	TTCGAA	1 cut(s)	1116
BsrDI	GCAATG	3 cut(s)	1071, 1281, 1302
BsrFI	RCCGGY	1 cut(s)	1526
BsrI	ACTGG	3 cut(s)	243, 1047, 1581
BssAI	RCCGGY	1 cut(s)	1526
BssECI	CCNNGG	6 cut(s)	6, 370, 431, 623, 681, 689
BssMI	GATC	3 cut(s)	194, 685, 1624
BssNI	GRCGYC	1 cut(s)	11
BssSI	CACGAG	1 cut(s)	828
BssT1I	CCWWGG	2 cut(s)	6, 370
Bst2BI	CACGAG	1 cut(s)	828
Bst2UI	CCWGG	7 cut(s)	624, 656, 683, 690, 696, 869, 1647
Bst4CI	ACNGT	2 cut(s)	1390, 1426
Bst6I	CTCTTC	1 cut(s)	435
BstACI	GRCGYC	1 cut(s)	11
BstAPI	GCANNNNNTGC	3 cut(s)	830, 992, 1271
BstBI	TTCGAA	1 cut(s)	1116
BstC8I	GCNNGC	4 cut(s)	96, 161, 201, 1267
BstDEI	CTNAG	4 cut(s)	187, 742, 943, 1192
BstDSI	CCRYGG	2 cut(s)	6, 370
BstF5I	GGATG	9 cut(s)	328, 512, 613, 638, 642, 985, 1051, 1312, 1372
BstFNI	CGCG	1 cut(s)	722
BstH2I	RGCGCY	1 cut(s)	49
BstHHI	GCGC	2 cut(s)	48, 722
BstKTI	GATC	3 cut(s)	197, 688, 1627
BstMAI	GTCTC	4 cut(s)	190, 540, 788, 1468
BstMBI	GATC	3 cut(s)	194, 685, 1624
BstMCI	CGRYCG	1 cut(s)	174
BstMWI	GCNNNNNNNGC	6 cut(s)	188, 245, 830, 992, 1262, 1271
BstNI	CCWGG	7 cut(s)	624, 656, 683, 690, 696, 869, 1647
BstNSI	RCATGY	3 cut(s)	565, 1269, 1524
BstSCI	CCNGG	8 cut(s)	430, 622, 654, 681, 688, 694, 867, 1645
BstSFI	CTRYAG	1 cut(s)	846
BstUI	CGCG	1 cut(s)	722
BstV1I	GCAGC	4 cut(s)	150, 832, 995, 1628
BstV2I	GAAGAC	2 cut(s)	1218, 1601
BstX2I	RGATCY	2 cut(s)	685, 1624
BstXI	CCANNNNNNTGG	1 cut(s)	94
BstYI	RGATCY	2 cut(s)	685, 1624
BsuRI	GGCC	2 cut(s)	5, 694
BtgI	CCRYGG	2 cut(s)	6, 370
BtsCI	GGATG	9 cut(s)	328, 512, 613, 638, 642, 985, 1051, 1312, 1372
BtsIMutI	CAGTG	3 cut(s)	250, 951, 1431
Cac8I	GCNNGC	4 cut(s)	96, 161, 201, 1267
CaiI	CAGNNNCTG	1 cut(s)	695
CfoI	GCGC	2 cut(s)	48, 722
Cfr10I	RCCGGY	1 cut(s)	1526
Cfr13I	GGNCC	4 cut(s)	220, 692, 1350, 1492
Csp6I	GTAC	3 cut(s)	494, 1524, 1545
CspAI	ACCGGT	1 cut(s)	1526
CviQI	GTAC	3 cut(s)	494, 1524, 1545
DdeI	CTNAG	4 cut(s)	187, 742, 943, 1192
DpnI	GATC	3 cut(s)	196, 687, 1626
DpnII	GATC	3 cut(s)	194, 685, 1624
DraIII	CACNNNGTG	2 cut(s)	233, 896
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	1 cut(s)	435
EarI	CTCTTC	1 cut(s)	435
EciI	GGCGGA	4 cut(s)	20, 55, 197, 519
Ecl136II	GAGCTC	1 cut(s)	159
Eco130I	CCWWGG	2 cut(s)	6, 370
Eco24I	GRGCYC	2 cut(s)	131, 161
Eco32I	GATATC	1 cut(s)	1344
Eco47I	GGWCC	3 cut(s)	220, 1350, 1492
Eco47III	AGCGCT	1 cut(s)	47
Eco53kI	GAGCTC	1 cut(s)	159
Eco57I	CTGAAG	3 cut(s)	459, 908, 1239
EcoICRI	GAGCTC	1 cut(s)	159
EcoO109I	RGGNCCY	1 cut(s)	692
EcoRII	CCWGG	7 cut(s)	622, 654, 681, 688, 694, 867, 1645
EcoRV	GATATC	1 cut(s)	1344
EcoT14I	CCWWGG	2 cut(s)	6, 370
EcoT22I	ATGCAT	1 cut(s)	1372
EcoT38I	GRGCYC	2 cut(s)	131, 161
ErhI	CCWWGG	2 cut(s)	6, 370
FalI	AAGNNNNNCTT	2 cut(s)	876, 908
FaqI	GGGAC	1 cut(s)	278
FauI	CCCGC	1 cut(s)	872
FauNDI	CATATG	1 cut(s)	499
Fnu4HI	GCNGC	4 cut(s)	164, 846, 984, 1617
FokI	GGATG	9 cut(s)	335, 499, 620, 645, 649, 992, 1058, 1319, 1379
FriOI	GRGCYC	2 cut(s)	131, 161
Fsp4HI	GCNGC	4 cut(s)	164, 846, 984, 1617
FspBI	CTAG	1 cut(s)	834
GlaI	GCGC	2 cut(s)	47, 721
GluI	GCNGC	4 cut(s)	164, 846, 984, 1617
HaeII	RGCGCY	1 cut(s)	49
HaeIII	GGCC	2 cut(s)	5, 694
HapII	CCGG	4 cut(s)	170, 192, 431, 1527
HhaI	GCGC	2 cut(s)	48, 722
Hin1I	GRCGYC	1 cut(s)	11
Hin6I	GCGC	2 cut(s)	46, 720
HinP1I	GCGC	2 cut(s)	46, 720
HinfI	GANTC	3 cut(s)	574, 1429, 1642
HpaII	CCGG	4 cut(s)	170, 192, 431, 1527
HphI	GGTGA	4 cut(s)	49, 883, 1418, 1541
Hpy166II	GTNNAC	2 cut(s)	87, 1532
Hpy188I	TCNGA	8 cut(s)	74, 124, 439, 927, 1180, 1195, 1306, 1375
Hpy188III	TCNNGA	5 cut(s)	332, 413, 1094, 1237, 1565
Hpy8I	GTNNAC	2 cut(s)	87, 1532
Hpy99I	CGWCG	2 cut(s)	16, 1464
HpyAV	CCTTC	6 cut(s)	313, 878, 932, 978, 1243, 1508
HpyCH4III	ACNGT	2 cut(s)	1390, 1426
HpyCH4IV	ACGT	1 cut(s)	727
HpyF10VI	GCNNNNNNNGC	6 cut(s)	188, 245, 830, 992, 1262, 1271
HpyF3I	CTNAG	4 cut(s)	187, 742, 943, 1192
HpySE526I	ACGT	1 cut(s)	727
Hsp92I	GRCGYC	1 cut(s)	11
HspAI	GCGC	2 cut(s)	46, 720
Kzo9I	GATC	3 cut(s)	194, 685, 1624
LguI	GCTCTTC	1 cut(s)	435
LmnI	GCTCC	4 cut(s)	506, 640, 664, 1279
Lsp1109I	GCAGC	4 cut(s)	150, 832, 995, 1628
LweI	GCATC	4 cut(s)	833, 970, 1357, 1379
MaeI	CTAG	1 cut(s)	834
MaeII	ACGT	1 cut(s)	727
MaeIII	GTNAC	3 cut(s)	524, 910, 1406
MalI	GATC	3 cut(s)	196, 687, 1626
MboI	GATC	3 cut(s)	194, 685, 1624
MboII	GAAGA	8 cut(s)	43, 46, 452, 1096, 1223, 1252, 1463, 1606
MfeI	CAATTG	3 cut(s)	215, 489, 705
MflI	RGATCY	2 cut(s)	685, 1624
MhlI	GDGCHC	2 cut(s)	131, 161
MlsI	TGGCCA	1 cut(s)	5
MluNI	TGGCCA	1 cut(s)	5
MmeI	TCCRAC	1 cut(s)	1127
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	1 cut(s)	1372
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	9 cut(s)	273, 405, 569, 582, 645, 816, 975, 1109, 1678
MslI	CAYNNNNRTG	4 cut(s)	344, 422, 560, 1404
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	1 cut(s)	1363
MspI	CCGG	4 cut(s)	170, 192, 431, 1527
MspR9I	CCNGG	8 cut(s)	432, 624, 656, 683, 690, 696, 869, 1647
MunI	CAATTG	3 cut(s)	215, 489, 705
Mva1269I	GAATGC	1 cut(s)	1017
MvaI	CCWGG	7 cut(s)	624, 656, 683, 690, 696, 869, 1647
MvnI	CGCG	1 cut(s)	722
MwoI	GCNNNNNNNGC	6 cut(s)	188, 245, 830, 992, 1262, 1271
NciI	CCSGG	1 cut(s)	432
NcoI	CCATGG	2 cut(s)	6, 370
NdeI	CATATG	1 cut(s)	499
NdeII	GATC	3 cut(s)	194, 685, 1624
NlaIV	GGNNCC	7 cut(s)	222, 486, 660, 687, 739, 760, 1281
NmuCI	GTSAC	3 cut(s)	524, 910, 1406
NsiI	ATGCAT	1 cut(s)	1372
NspI	RCATGY	3 cut(s)	565, 1269, 1524
NspV	TTCGAA	1 cut(s)	1116
PaeI	GCATGC	1 cut(s)	1269
PciI	ACATGT	2 cut(s)	561, 1520
PciSI	GCTCTTC	1 cut(s)	435
PcsI	WCGNNNNNNNCGW	1 cut(s)	31
PctI	GAATGC	1 cut(s)	1017
PfeI	GAWTC	3 cut(s)	574, 1429, 1642
PflMI	CCANNNNNTGG	2 cut(s)	695, 1489
PfoI	TCCNGGA	1 cut(s)	1645
PinAI	ACCGGT	1 cut(s)	1526
PkrI	GCNGC	4 cut(s)	165, 847, 985, 1618
PscI	ACATGT	2 cut(s)	561, 1520
PshBI	ATTAAT	1 cut(s)	569
Psp124BI	GAGCTC	1 cut(s)	161
Psp6I	CCWGG	7 cut(s)	622, 654, 681, 688, 694, 867, 1645
PspGI	CCWGG	7 cut(s)	622, 654, 681, 688, 694, 867, 1645
PspN4I	GGNNCC	7 cut(s)	222, 486, 660, 687, 739, 760, 1281
PspPI	GGNCC	4 cut(s)	220, 692, 1350, 1492
PsrI	GAACNNNNNNTAC	2 cut(s)	94, 126
PstI	CTGCAG	1 cut(s)	850
PstNI	CAGNNNCTG	1 cut(s)	695
PsuI	RGATCY	2 cut(s)	685, 1624
PvuII	CAGCTG	1 cut(s)	1363
RsaI	GTAC	3 cut(s)	495, 1525, 1546
RsaNI	GTAC	3 cut(s)	494, 1524, 1545
RseI	CAYNNNNRTG	4 cut(s)	344, 422, 560, 1404
SacI	GAGCTC	1 cut(s)	161
SapI	GCTCTTC	1 cut(s)	435
SaqAI	TTAA	9 cut(s)	273, 405, 569, 582, 645, 816, 975, 1109, 1678
SatI	GCNGC	4 cut(s)	164, 846, 984, 1617
Sau3AI	GATC	3 cut(s)	194, 685, 1624
Sau96I	GGNCC	4 cut(s)	220, 692, 1350, 1492
ScrFI	CCNGG	8 cut(s)	432, 624, 656, 683, 690, 696, 869, 1647
SduI	GDGCHC	2 cut(s)	131, 161
SfaNI	GCATC	4 cut(s)	833, 970, 1357, 1379
SfcI	CTRYAG	1 cut(s)	846
SfuI	TTCGAA	1 cut(s)	1116
SinI	GGWCC	3 cut(s)	220, 1350, 1492
SmiMI	CAYNNNNRTG	4 cut(s)	344, 422, 560, 1404
SmlI	CTYRAG	2 cut(s)	785, 1565
SmoI	CTYRAG	2 cut(s)	785, 1565
SphI	GCATGC	1 cut(s)	1269
SsiI	CCGC	9 cut(s)	31, 66, 208, 380, 401, 504, 865, 935, 1208
SspI	AATATT	1 cut(s)	409
SspMI	CTAG	1 cut(s)	834
SstI	GAGCTC	1 cut(s)	161
StyD4I	CCNGG	8 cut(s)	430, 622, 654, 681, 688, 694, 867, 1645
StyI	CCWWGG	2 cut(s)	6, 370
TaaI	ACNGT	2 cut(s)	1390, 1426
TaiI	ACGT	1 cut(s)	730
TaqI	TCGA	3 cut(s)	155, 1116, 1459
TfiI	GAWTC	3 cut(s)	574, 1429, 1642
Tru1I	TTAA	9 cut(s)	273, 405, 569, 582, 645, 816, 975, 1109, 1678
Tru9I	TTAA	9 cut(s)	273, 405, 569, 582, 645, 816, 975, 1109, 1678
TscAI	CASTG	3 cut(s)	250, 951, 1431
TseFI	GTSAC	3 cut(s)	524, 910, 1406
TseI	GCWGC	4 cut(s)	163, 845, 983, 1616
Tsp45I	GTSAC	3 cut(s)	524, 910, 1406
TspDTI	ATGAA	7 cut(s)	327, 587, 663, 1464, 1571, 1616, 1655
TspGWI	ACGGA	1 cut(s)	920
TspRI	CASTG	3 cut(s)	250, 951, 1431
Van91I	CCANNNNNTGG	2 cut(s)	695, 1489
VpaK11BI	GGWCC	3 cut(s)	220, 1350, 1492
VspI	ATTAAT	1 cut(s)	569
XapI	RAATTY	4 cut(s)	250, 930, 1087, 1118
XceI	RCATGY	3 cut(s)	565, 1269, 1524
XcmI	CCANNNNNNNNNTGG	2 cut(s)	147, 1360
XspI	CTAG	1 cut(s)	834
Zsp2I	ATGCAT	1 cut(s)	1372

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.