Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0007744

Physical Location & Seq

Genomic Coordinates Reverse (-)

5619310 .. 5624217

Gene Structure 4908 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1049100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1221 bp

ATGAGACCCAACATCGTTTCCGAGGCAGGACTGCAAACTAGGTTGAGGCAATGGTGGGAAAGCATTCCTTTTCTTACTTCAGCGGTGGTCGGCGTGTGCGCGACTATTTACTTGGTTTGCCTCCTGGTTGGATATGACTCCTTTTACGAAGTGTGCTTTTCACCCTCTGATGTTGTATCCCGATTCCAAGTCTACAGGATTTTCACCTCCATTATCTTCCATGGTTCAGTGCTGCATGTGTTGTTCAACATGATGGCATTAGTTCCTTTGGGTTCTGAGTTGGAGAGAATCATGGGAACTGTCCGCTTGTTGTACATGATCATTCTATTGGCCATAAGCAATGCAGTATTACATCTTCTGATTGCGCTTTTGGTATCCTATAACCCTATTCACCCTTCTCAGTACCTCATGGATGAATGTGCAATAGGCTTCTCGGGAATCTTGTTTTCTATGATTGTTATAGAGACAAGTCTGAGTGGACACCAGTCTAGAAGCGTGTTTGGACTCTTCAATGTACCTGCTAAATGGTATGCATGGATCTTGCTGGTAGTGTTCCAAGTTGTTATGCCAAATGTTTCTTTACTGGGACACCTATGTGGCATTTTGTCTGGCTTTGCTTATACTTACAGCTTATTAAATTTCCTCATTCCGGGAACATCCTTCTATTCTAAAATTGAGGCCTCCTCATTGTTTTCAAGTTGTGTGAGGAGGCCTAAATTTATTGTGTGCACTGGTGGGAATCCTTCTGCCTTCATCCCTACATATTCAACCCAAGGTACACCCTCTAGTGGATTCTCTGCTGGAAGTATCTGGAGAAACTTGTCTTCATGGATCCCACAGAGGGAAACATCTGTACAGTCAGCACAAGATGGTCACATGTTTCCTGGGAGAGGAAGAACACTTGGATCTGGTCGAGGTACTATTTCTGATGCTGATTCAGAATCAAACTTACAGGCTAGACTCTTGGACAATAGCAACTCAGAACGTCCATCAGATGCGGCAGTTATTGGTACAGGACAGAATTTATCTGATGGAAGGCAGGCAACACTAAATAATGCAGCGGCAGAAATTCCTGTACACCATCAGGATTATGTTGCATCTGAAGAAGAAATCCAAAGGCTTGTATCAATGGGTTTTGAGAGGACACAGGTAGAAGTTGCCCTAGCAGCTGCAGATGGGGATCTTAATGTGGCAGTGGAGATTCTGAGCCAACAGGGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Rhomboid UBA

Protein Analysis

407

Amino Acids

44.37

Weight (kDa)

5.71

Isoelectric Point (pI)

45.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DER1	PF04511	22 - 209	1.5e-08	Der1-like family
Rhomboid	PF01694	62 - 212	1.6e-27	Rhomboid domain
UBA	PF00627	368 - 402	3.7e-10	UBA/TS-N domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007744)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G58460 AT3G58460
fragaria_vesca	FvH4_6g21260 FvH4_6g21260 FvH4_6g21260 FvH4_6g21260 FvH4_6g21260 FvH4_6g21260 FvH4_6g21260
malus_domestica	MD12G1049100.v1.1 MD14G1048000.v1.1
prunus_persica	Prupe.7G087100_v2.0.a1
pyrus_communis	pycom12g04400 pycom14g03950
rosa_chinensis	RchiOBHm_Chr3g0477721
rosa_laevigata	RLG00000023695
rosa_multiflora	Rmu_sc0006187.1_g000017
rosa_roxburghii	Rroxscaffold_6G00403720
rosa_rugosa	Rorug03G0162900
rosa_samantha	Rh3AG213200 Rh3BG246900 Rh3CG240700 Rh3DG240000
rosa_wichuraiana	Rw3G019300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	526
AccI	GTMKAC	1 cut(s)	192
AccII	CGCG	1 cut(s)	101
AciI	CCGC	4 cut(s)	83, 304, 998, 1061
AclWI	GGATC	5 cut(s)	545, 826, 839, 913, 1188
AcoI	YGGCCR	1 cut(s)	330
AcsI	RAATTY	4 cut(s)	637, 716, 1021, 1068
AcuI	CTGAAG	2 cut(s)	63, 1122
AfaI	GTAC	8 cut(s)	314, 404, 516, 778, 855, 919, 1012, 1077
AfiI	CCNNNNNNNGG	4 cut(s)	649, 788, 841, 890
AflIII	ACRYGT	1 cut(s)	876
AgsI	TTSAA	4 cut(s)	247, 511, 696, 768
AjnI	CCWGG	2 cut(s)	123, 883
AleI	CACNNNNGTG	1 cut(s)	594
AluBI	AGCT	2 cut(s)	630, 1169
AluI	AGCT	2 cut(s)	630, 1169
Alw21I	GWGCWC	1 cut(s)	731
Alw26I	GTCTC	1 cut(s)	458
Alw44I	GTGCAC	1 cut(s)	727
AlwI	GGATC	5 cut(s)	545, 826, 839, 913, 1188
Ama87I	CYCGRG	1 cut(s)	433
AoxI	GGCC	3 cut(s)	330, 678, 710
ApaLI	GTGCAC	1 cut(s)	727
ApeKI	GCWGC	4 cut(s)	232, 1058, 1166, 1169
ApoI	RAATTY	4 cut(s)	637, 716, 1021, 1068
Asp700I	GAANNNNTTC	1 cut(s)	63
AspLEI	GCGC	2 cut(s)	101, 367
AsuC2I	CCSGG	1 cut(s)	651
AsuHPI	GGTGA	3 cut(s)	153, 196, 383
AvaI	CYCGRG	1 cut(s)	433
BaeGI	GKGCMC	1 cut(s)	731
BalI	TGGCCA	1 cut(s)	332
BamHI	GGATCC	1 cut(s)	831
BarI	GAAGNNNNNNTAC	2 cut(s)	339, 371
BbsI	GAAGAC	1 cut(s)	816
Bbv12I	GWGCWC	1 cut(s)	731
BbvI	GCAGC	4 cut(s)	219, 1070, 1156, 1178
BccI	CCATC	6 cut(s)	247, 863, 997, 1025, 1089, 1169
BciT130I	CCWGG	2 cut(s)	125, 885
BciVI	GTATCC	2 cut(s)	187, 385
BclI	TGATCA	1 cut(s)	318
BcnI	CCSGG	1 cut(s)	651
BcoDI	GTCTC	1 cut(s)	458
BfaI	CTAG	5 cut(s)	39, 489, 786, 957, 1163
BfmI	CTRYAG	2 cut(s)	193, 1170
BfuAI	ACCTGC	1 cut(s)	526
BfuI	GTATCC	2 cut(s)	187, 385
BglI	GCCNNNNNGGC	1 cut(s)	1215
BisI	GCNGC	6 cut(s)	233, 999, 1059, 1062, 1167, 1170
BlsI	GCNGC	6 cut(s)	234, 1000, 1060, 1063, 1168, 1171
Bme1390I	CCNGG	3 cut(s)	125, 651, 885
BmeT110I	CYCGRG	1 cut(s)	433
BmiI	GGNNCC	1 cut(s)	833
BmrFI	CCNGG	3 cut(s)	125, 651, 885
BmrI	ACTGGG	1 cut(s)	593
BmsI	GCATC	3 cut(s)	919, 985, 1106
BmuI	ACTGGG	1 cut(s)	593
BoxI	GACNNNNGTC	1 cut(s)	484
BpiI	GAAGAC	1 cut(s)	816
BplI	GAGNNNNNCTC	2 cut(s)	668, 700
BpmI	CTGGAG	1 cut(s)	832
BpuMI	CCSGG	1 cut(s)	651
BsaBI	GATNNNNATC	1 cut(s)	1179
BsaJI	CCNNGG	4 cut(s)	21, 220, 772, 884
Bsc4I	CCNNNNNNNGG	4 cut(s)	649, 788, 841, 890
Bse1I	ACTGG	3 cut(s)	484, 588, 736
Bse3DI	GCAATG	2 cut(s)	56, 346
Bse8I	GATNNNNATC	1 cut(s)	1179
BseBI	CCWGG	2 cut(s)	125, 885
BseDI	CCNNGG	4 cut(s)	21, 220, 772, 884
BseGI	GGATG	3 cut(s)	418, 656, 753
BseJI	GATNNNNATC	1 cut(s)	1179
BseLI	CCNNNNNNNGG	4 cut(s)	649, 788, 841, 890
BseMI	GCAATG	2 cut(s)	56, 346
BseMII	CTCAG	5 cut(s)	267, 413, 464, 993, 1196
BseNI	ACTGG	3 cut(s)	484, 588, 736
BseRI	GAGGAG	2 cut(s)	673, 721
BseSI	GKGCMC	1 cut(s)	731
BseXI	GCAGC	4 cut(s)	219, 1070, 1156, 1178
Bsh1236I	CGCG	1 cut(s)	101
BshFI	GGCC	3 cut(s)	332, 680, 712
BsiHKAI	GWGCWC	1 cut(s)	731
BsiHKCI	CYCGRG	1 cut(s)	433
BsiSI	CCGG	1 cut(s)	650
BslFI	GGGAC	1 cut(s)	600
BslI	CCNNNNNNNGG	4 cut(s)	649, 788, 841, 890
BsmAI	GTCTC	1 cut(s)	458
BsmFI	GGGAC	1 cut(s)	600
BsmI	GAATGC	1 cut(s)	63
BsnI	GGCC	3 cut(s)	332, 680, 712
BsoBI	CYCGRG	1 cut(s)	433
Bsp1286I	GDGCHC	1 cut(s)	731
Bsp1407I	TGTACA	3 cut(s)	312, 853, 1075
Bsp143I	GATC	5 cut(s)	318, 537, 831, 905, 1180
Bsp19I	CCATGG	1 cut(s)	220
BspACI	CCGC	4 cut(s)	83, 304, 998, 1061
BspANI	GGCC	3 cut(s)	332, 680, 712
BspCNI	CTCAG	5 cut(s)	268, 412, 465, 992, 1197
BspFNI	CGCG	1 cut(s)	101
BspLI	GGNNCC	1 cut(s)	833
BspMAI	CTGCAG	1 cut(s)	1174
BspMI	ACCTGC	1 cut(s)	526
BspPI	GGATC	5 cut(s)	545, 826, 839, 913, 1188
BsrDI	GCAATG	2 cut(s)	56, 346
BsrGI	TGTACA	3 cut(s)	312, 853, 1075
BsrI	ACTGG	3 cut(s)	484, 588, 736
BssECI	CCNNGG	4 cut(s)	21, 220, 772, 884
BssMI	GATC	5 cut(s)	318, 537, 831, 905, 1180
BssT1I	CCWWGG	2 cut(s)	220, 772
Bst2UI	CCWGG	2 cut(s)	125, 885
Bst4CI	ACNGT	2 cut(s)	301, 858
Bst6I	CTCTTC	1 cut(s)	512
BstAUI	TGTACA	3 cut(s)	312, 853, 1075
BstC8I	GCNNGC	1 cut(s)	1041
BstDEI	CTNAG	5 cut(s)	276, 399, 473, 979, 1205
BstDSI	CCRYGG	1 cut(s)	220
BstENI	CCTNNNNNAGG	1 cut(s)	888
BstF5I	GGATG	3 cut(s)	418, 656, 753
BstFNI	CGCG	1 cut(s)	101
BstHHI	GCGC	2 cut(s)	101, 367
BstKTI	GATC	5 cut(s)	321, 540, 834, 908, 1183
BstMAI	GTCTC	1 cut(s)	458
BstMBI	GATC	5 cut(s)	318, 537, 831, 905, 1180
BstMWI	GCNNNNNNNGC	2 cut(s)	1166, 1215
BstNI	CCWGG	2 cut(s)	125, 885
BstNSI	RCATGY	2 cut(s)	239, 880
BstPAI	GACNNNNGTC	1 cut(s)	484
BstSCI	CCNGG	3 cut(s)	123, 649, 883
BstSFI	CTRYAG	2 cut(s)	193, 1170
BstSLI	GKGCMC	1 cut(s)	731
BstUI	CGCG	1 cut(s)	101
BstV1I	GCAGC	4 cut(s)	219, 1070, 1156, 1178
BstV2I	GAAGAC	1 cut(s)	816
BstX2I	RGATCY	4 cut(s)	537, 831, 905, 1180
BstYI	RGATCY	4 cut(s)	537, 831, 905, 1180
BsuI	GTATCC	2 cut(s)	187, 385
BsuRI	GGCC	3 cut(s)	332, 680, 712
BtgI	CCRYGG	1 cut(s)	220
BtsCI	GGATG	3 cut(s)	418, 656, 753
BtsI	GCAGTG	1 cut(s)	1200
BtsIMutI	CAGTG	3 cut(s)	234, 729, 1200
BveI	ACCTGC	1 cut(s)	526
Cac8I	GCNNGC	1 cut(s)	1041
CfoI	GCGC	2 cut(s)	101, 367
Csp6I	GTAC	8 cut(s)	313, 403, 515, 777, 854, 918, 1011, 1076
CviAII	CATG	9 cut(s)	221, 236, 250, 292, 316, 409, 534, 828, 877
CviQI	GTAC	8 cut(s)	313, 403, 515, 777, 854, 918, 1011, 1076
DdeI	CTNAG	5 cut(s)	276, 399, 473, 979, 1205
DpnI	GATC	5 cut(s)	320, 539, 833, 907, 1182
DpnII	GATC	5 cut(s)	318, 537, 831, 905, 1180
EaeI	YGGCCR	1 cut(s)	330
Eam1104I	CTCTTC	1 cut(s)	512
EarI	CTCTTC	1 cut(s)	512
Eco130I	CCWWGG	2 cut(s)	220, 772
Eco147I	AGGCCT	2 cut(s)	680, 712
Eco57I	CTGAAG	2 cut(s)	63, 1122
Eco88I	CYCGRG	1 cut(s)	433
EcoNI	CCTNNNNNAGG	1 cut(s)	888
EcoRII	CCWGG	2 cut(s)	123, 883
EcoT14I	CCWWGG	2 cut(s)	220, 772
EcoT22I	ATGCAT	1 cut(s)	535
ErhI	CCWWGG	2 cut(s)	220, 772
FaeI	CATG	9 cut(s)	224, 239, 253, 295, 319, 412, 537, 831, 880
FalI	AAGNNNNNCTT	2 cut(s)	52, 84
FaqI	GGGAC	1 cut(s)	600
FatI	CATG	9 cut(s)	220, 235, 249, 291, 315, 408, 533, 827, 876
FbaI	TGATCA	1 cut(s)	318
FblI	GTMKAC	1 cut(s)	192
Fnu4HI	GCNGC	6 cut(s)	233, 999, 1059, 1062, 1167, 1170
FokI	GGATG	3 cut(s)	425, 643, 740
Fsp4HI	GCNGC	6 cut(s)	233, 999, 1059, 1062, 1167, 1170
FspBI	CTAG	5 cut(s)	39, 489, 786, 957, 1163
GlaI	GCGC	2 cut(s)	100, 366
GluI	GCNGC	6 cut(s)	233, 999, 1059, 1062, 1167, 1170
GsuI	CTGGAG	1 cut(s)	832
HaeIII	GGCC	3 cut(s)	332, 680, 712
HapII	CCGG	1 cut(s)	650
HhaI	GCGC	2 cut(s)	101, 367
Hin1II	CATG	9 cut(s)	224, 239, 253, 295, 319, 412, 537, 831, 880
Hin6I	GCGC	2 cut(s)	99, 365
HinP1I	GCGC	2 cut(s)	99, 365
HpaII	CCGG	1 cut(s)	650
HphI	GGTGA	3 cut(s)	153, 196, 383
Hpy166II	GTNNAC	5 cut(s)	193, 479, 729, 779, 1078
Hpy188III	TCNNGA	5 cut(s)	180, 435, 489, 811, 1085
Hpy8I	GTNNAC	5 cut(s)	193, 479, 729, 779, 1078
HpyAV	CCTTC	5 cut(s)	405, 670, 753, 760, 1029
HpyCH4III	ACNGT	2 cut(s)	301, 858
HpyCH4IV	ACGT	1 cut(s)	985
HpyCH4V	TGCA	9 cut(s)	34, 235, 344, 422, 533, 729, 1058, 1097, 1172
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1166, 1215
HpyF3I	CTNAG	5 cut(s)	276, 399, 473, 979, 1205
HpySE526I	ACGT	1 cut(s)	985
Hsp92II	CATG	9 cut(s)	224, 239, 253, 295, 319, 412, 537, 831, 880
HspAI	GCGC	2 cut(s)	99, 365
Ksp22I	TGATCA	1 cut(s)	318
Kzo9I	GATC	5 cut(s)	318, 537, 831, 905, 1180
Lsp1109I	GCAGC	4 cut(s)	219, 1070, 1156, 1178
LweI	GCATC	3 cut(s)	919, 985, 1106
MaeI	CTAG	5 cut(s)	39, 489, 786, 957, 1163
MaeII	ACGT	1 cut(s)	985
MaeIII	GTNAC	1 cut(s)	872
MalI	GATC	5 cut(s)	320, 539, 833, 907, 1182
MboI	GATC	5 cut(s)	318, 537, 831, 905, 1180
MboII	GAAGA	7 cut(s)	208, 347, 499, 816, 906, 1115, 1118
MflI	RGATCY	4 cut(s)	537, 831, 905, 1180
MhlI	GDGCHC	1 cut(s)	731
MlsI	TGGCCA	1 cut(s)	332
MluCI	AATT	5 cut(s)	637, 672, 716, 1021, 1068
MluNI	TGGCCA	1 cut(s)	332
MlyI	GAGTC	3 cut(s)	131, 498, 954
MmeI	TCCRAC	2 cut(s)	109, 261
Mox20I	TGGCCA	1 cut(s)	332
Mph1103I	ATGCAT	1 cut(s)	535
MroXI	GAANNNNTTC	1 cut(s)	63
MscI	TGGCCA	1 cut(s)	332
MseI	TTAA	2 cut(s)	635, 1185
MslI	CAYNNNNRTG	1 cut(s)	594
Msp20I	TGGCCA	1 cut(s)	332
MspA1I	CMGCKG	3 cut(s)	83, 1061, 1169
MspI	CCGG	1 cut(s)	650
MspR9I	CCNGG	3 cut(s)	125, 651, 885
Mva1269I	GAATGC	1 cut(s)	63
MvaI	CCWGG	2 cut(s)	125, 885
MvnI	CGCG	1 cut(s)	101
MwoI	GCNNNNNNNGC	2 cut(s)	1166, 1215
NciI	CCSGG	1 cut(s)	651
NcoI	CCATGG	1 cut(s)	220
NdeII	GATC	5 cut(s)	318, 537, 831, 905, 1180
NlaIII	CATG	9 cut(s)	224, 239, 253, 295, 319, 412, 537, 831, 880
NlaIV	GGNNCC	1 cut(s)	833
NmuCI	GTSAC	1 cut(s)	872
NsiI	ATGCAT	1 cut(s)	535
NspI	RCATGY	2 cut(s)	239, 880
OliI	CACNNNNGTG	1 cut(s)	594
PceI	AGGCCT	2 cut(s)	680, 712
PciI	ACATGT	1 cut(s)	876
PctI	GAATGC	1 cut(s)	63
PdmI	GAANNNNTTC	1 cut(s)	63
PfeI	GAWTC	8 cut(s)	183, 288, 438, 739, 792, 935, 941, 1201
PfoI	TCCNGGA	1 cut(s)	649
PkrI	GCNGC	6 cut(s)	234, 1000, 1060, 1063, 1168, 1171
PleI	GAGTC	3 cut(s)	131, 498, 954
PpsI	GAGTC	3 cut(s)	131, 498, 954
PscI	ACATGT	1 cut(s)	876
PshAI	GACNNNNGTC	1 cut(s)	484
Psp6I	CCWGG	2 cut(s)	123, 883
PspGI	CCWGG	2 cut(s)	123, 883
PspN4I	GGNNCC	1 cut(s)	833
PstI	CTGCAG	1 cut(s)	1174
PsuI	RGATCY	4 cut(s)	537, 831, 905, 1180
PvuII	CAGCTG	1 cut(s)	1169
RsaI	GTAC	8 cut(s)	314, 404, 516, 778, 855, 919, 1012, 1077
RsaNI	GTAC	8 cut(s)	313, 403, 515, 777, 854, 918, 1011, 1076
RseI	CAYNNNNRTG	1 cut(s)	594
SaqAI	TTAA	2 cut(s)	635, 1185
SatI	GCNGC	6 cut(s)	233, 999, 1059, 1062, 1167, 1170
Sau3AI	GATC	5 cut(s)	318, 537, 831, 905, 1180
SchI	GAGTC	3 cut(s)	131, 498, 954
ScrFI	CCNGG	3 cut(s)	125, 651, 885
SduI	GDGCHC	1 cut(s)	731
SfaNI	GCATC	3 cut(s)	919, 985, 1106
SfcI	CTRYAG	2 cut(s)	193, 1170
SmiMI	CAYNNNNRTG	1 cut(s)	594
Sse9I	AATT	5 cut(s)	637, 672, 716, 1021, 1068
SseBI	AGGCCT	2 cut(s)	680, 712
SsiI	CCGC	4 cut(s)	83, 304, 998, 1061
SspMI	CTAG	5 cut(s)	39, 489, 786, 957, 1163
StuI	AGGCCT	2 cut(s)	680, 712
StyD4I	CCNGG	3 cut(s)	123, 649, 883
StyI	CCWWGG	2 cut(s)	220, 772
TaaI	ACNGT	2 cut(s)	301, 858
TaiI	ACGT	1 cut(s)	988
TaqI	TCGA	1 cut(s)	913
TasI	AATT	5 cut(s)	637, 672, 716, 1021, 1068
TatI	WGTACW	3 cut(s)	312, 853, 1075
TauI	GCSGC	2 cut(s)	1001, 1064
TfiI	GAWTC	8 cut(s)	183, 288, 438, 739, 792, 935, 941, 1201
Tru1I	TTAA	2 cut(s)	635, 1185
Tru9I	TTAA	2 cut(s)	635, 1185
TscAI	CASTG	3 cut(s)	234, 736, 1200
TseFI	GTSAC	1 cut(s)	872
TseI	GCWGC	4 cut(s)	232, 1058, 1166, 1169
Tsp45I	GTSAC	1 cut(s)	872
TspDTI	ATGAA	3 cut(s)	429, 742, 816
TspRI	CASTG	3 cut(s)	234, 736, 1200
VneI	GTGCAC	1 cut(s)	727
XagI	CCTNNNNNAGG	1 cut(s)	888
XapI	RAATTY	4 cut(s)	637, 716, 1021, 1068
XbaI	TCTAGA	1 cut(s)	488
XceI	RCATGY	2 cut(s)	239, 880
XmiI	GTMKAC	1 cut(s)	192
XmnI	GAANNNNTTC	1 cut(s)	63
XspI	CTAG	5 cut(s)	39, 489, 786, 957, 1163
Zsp2I	ATGCAT	1 cut(s)	535

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.