MD14G1048000.v1.1

Der1-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007744
Physical Location & Seq
Reverse (-)
4593132 .. 4596083
2952 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1048000.v1.1.491

Sequence Viewer

Length: 663 bp
ATGTTGTATCCAAATTCCAAGGTATGCATGGATCTTGCTGGTAGTGTTCCAAGTTATACTTACAGCTTATTAAATTTCCTCATTCCGGGAACATCCTTCTATTCTAAAATTGAGGCCTCCTCATTGTTTTCAAGTTGTGTAAGGCGGCCTAAATTTATTGTGTGCACTGGTGGGAATCCTTCAGCCTTCATCCCTACATATTCAACCCAAGGTACACCATGTAGTGGATTCTCTGCTGGAAGTATCTGGAGAAACTTGTCTCCGTGGATCCCACGTAGGGAAACATCTGTACAGTCAGCACAAGACGGTCACAGGTTTCCTGGGAGGGGAAGAACACTTGCATCTGGTCGAAGTACTGTTTCTGATGTGGATTCAGAATCAAATTTACAGGCTAGACTCTTGGACAATAGCAACTCAGAACGTCCATCAGATGTGGCAGTTATTGGTACAGGACAGCAGTTATCTGATGGAAGGCGGGCAACACTAAATAGTGCAGCAGCAGAAATTCCTGTACACCACCAGATGCAGGATTATGTTGCTTCTGAAGAAGAAATCAAAAAGCTTGTATCAATGGGTTTTGAGAGGACACAGGTAGAAGTTGCCCTAGCAGCTGCAGATGGGGATCTTAACGTGGCAGCGGAAATTCTGAGCCAACAGGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid UBA

Protein Analysis

221

Amino Acids

23.67

Weight (kDa)

5.93

Isoelectric Point (pI)

48.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA PF00627 182 - 216 4e-09 UBA/TS-N domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 224
AciI CCGC 3 cut(s) 145, 475, 638
AclWI GGATC 4 cut(s) 39, 262, 275, 630
AcsI RAATTY 6 cut(s) 13, 73, 152, 382, 504, 642
AcuI CTGAAG 2 cut(s) 165, 564
AfaI GTAC 5 cut(s) 214, 291, 355, 448, 513
AfiI CCNNNNNNNGG 5 cut(s) 85, 224, 277, 326, 526
AgsI TTSAA 2 cut(s) 132, 204
AjnI CCWGG 1 cut(s) 319
AluBI AGCT 3 cut(s) 66, 562, 611
AluI AGCT 3 cut(s) 66, 562, 611
Alw21I GWGCWC 1 cut(s) 167
Alw26I GTCTC 1 cut(s) 264
Alw44I GTGCAC 1 cut(s) 163
AlwI GGATC 4 cut(s) 39, 262, 275, 630
AoxI GGCC 2 cut(s) 114, 146
ApaLI GTGCAC 1 cut(s) 163
ApeKI GCWGC 5 cut(s) 494, 497, 608, 611, 635
ApoI RAATTY 6 cut(s) 13, 73, 152, 382, 504, 642
AsuC2I CCSGG 1 cut(s) 87
BaeGI GKGCMC 1 cut(s) 167
BamHI GGATCC 1 cut(s) 267
Bbv12I GWGCWC 1 cut(s) 167
BbvI GCAGC 5 cut(s) 506, 509, 598, 620, 647
BccI CCATC 3 cut(s) 433, 461, 611
BciT130I CCWGG 1 cut(s) 321
BciVI GTATCC 1 cut(s) 18
BcnI CCSGG 1 cut(s) 87
BcoDI GTCTC 1 cut(s) 264
BfaI CTAG 2 cut(s) 393, 605
BfmI CTRYAG 1 cut(s) 612
BfuI GTATCC 1 cut(s) 18
BglI GCCNNNNNGGC 1 cut(s) 657
BisI GCNGC 6 cut(s) 146, 495, 498, 609, 612, 636
BlsI GCNGC 6 cut(s) 147, 496, 499, 610, 613, 637
BmcAI AGTACT 1 cut(s) 355
Bme1390I CCNGG 2 cut(s) 87, 321
BmiI GGNNCC 1 cut(s) 269
BmrFI CCNGG 2 cut(s) 87, 321
BmsI GCATC 2 cut(s) 350, 513
BplI GAGNNNNNCTC 2 cut(s) 104, 136
BpmI CTGGAG 1 cut(s) 268
BpuMI CCSGG 1 cut(s) 87
BsaAI YACGTR 1 cut(s) 275
BsaBI GATNNNNATC 1 cut(s) 621
BsaJI CCNNGG 4 cut(s) 18, 208, 263, 320
Bsc4I CCNNNNNNNGG 5 cut(s) 85, 224, 277, 326, 526
Bse1I ACTGG 1 cut(s) 172
Bse8I GATNNNNATC 1 cut(s) 621
BseBI CCWGG 1 cut(s) 321
BseDI CCNNGG 4 cut(s) 18, 208, 263, 320
BseGI GGATG 2 cut(s) 92, 189
BseJI GATNNNNATC 1 cut(s) 621
BseLI CCNNNNNNNGG 5 cut(s) 85, 224, 277, 326, 526
BseMII CTCAG 2 cut(s) 429, 638
BseNI ACTGG 1 cut(s) 172
BseRI GAGGAG 1 cut(s) 109
BseSI GKGCMC 1 cut(s) 167
BseXI GCAGC 5 cut(s) 506, 509, 598, 620, 647
BsgI GTGCAG 1 cut(s) 513
BshFI GGCC 2 cut(s) 116, 148
BsiHKAI GWGCWC 1 cut(s) 167
BsiSI CCGG 1 cut(s) 86
BslI CCNNNNNNNGG 5 cut(s) 85, 224, 277, 326, 526
BsmAI GTCTC 1 cut(s) 264
BsnI GGCC 2 cut(s) 116, 148
Bsp1286I GDGCHC 1 cut(s) 167
Bsp1407I TGTACA 2 cut(s) 289, 511
Bsp143I GATC 3 cut(s) 31, 267, 622
BspACI CCGC 3 cut(s) 145, 475, 638
BspANI GGCC 2 cut(s) 116, 148
BspCNI CTCAG 2 cut(s) 428, 639
BspLI GGNNCC 1 cut(s) 269
BspMAI CTGCAG 1 cut(s) 616
BspPI GGATC 4 cut(s) 39, 262, 275, 630
BsrGI TGTACA 2 cut(s) 289, 511
BsrI ACTGG 1 cut(s) 172
BssECI CCNNGG 4 cut(s) 18, 208, 263, 320
BssMI GATC 3 cut(s) 31, 267, 622
BssT1I CCWWGG 2 cut(s) 18, 208
Bst2UI CCWGG 1 cut(s) 321
Bst4CI ACNGT 3 cut(s) 294, 308, 358
BstAUI TGTACA 2 cut(s) 289, 511
BstBAI YACGTR 1 cut(s) 275
BstC8I GCNNGC 1 cut(s) 477
BstDEI CTNAG 2 cut(s) 415, 647
BstDSI CCRYGG 1 cut(s) 263
BstF5I GGATG 2 cut(s) 92, 189
BstKTI GATC 3 cut(s) 34, 270, 625
BstMAI GTCTC 1 cut(s) 264
BstMBI GATC 3 cut(s) 31, 267, 622
BstMWI GCNNNNNNNGC 2 cut(s) 608, 657
BstNI CCWGG 1 cut(s) 321
BstSCI CCNGG 2 cut(s) 85, 319
BstSFI CTRYAG 1 cut(s) 612
BstSLI GKGCMC 1 cut(s) 167
BstV1I GCAGC 5 cut(s) 506, 509, 598, 620, 647
BstX2I RGATCY 3 cut(s) 31, 267, 622
BstYI RGATCY 3 cut(s) 31, 267, 622
BsuI GTATCC 1 cut(s) 18
BsuRI GGCC 2 cut(s) 116, 148
BtgI CCRYGG 1 cut(s) 263
BtsCI GGATG 2 cut(s) 92, 189
BtsIMutI CAGTG 1 cut(s) 165
Cac8I GCNNGC 1 cut(s) 477
Csp6I GTAC 5 cut(s) 213, 290, 354, 447, 512
CviAII CATG 2 cut(s) 28, 219
CviJI RGCY 9 cut(s) 66, 116, 148, 185, 392, 562, 611, 651, 660
CviKI_1 RGCY 9 cut(s) 66, 116, 148, 185, 392, 562, 611, 651, 660
CviQI GTAC 5 cut(s) 213, 290, 354, 447, 512
DdeI CTNAG 2 cut(s) 415, 647
DpnI GATC 3 cut(s) 33, 269, 624
DpnII GATC 3 cut(s) 31, 267, 622
Eco130I CCWWGG 2 cut(s) 18, 208
Eco147I AGGCCT 1 cut(s) 116
Eco57I CTGAAG 2 cut(s) 165, 564
EcoRII CCWGG 1 cut(s) 319
EcoT14I CCWWGG 2 cut(s) 18, 208
EcoT22I ATGCAT 1 cut(s) 29
ErhI CCWWGG 2 cut(s) 18, 208
FaeI CATG 2 cut(s) 31, 222
FaiI YATR 6 cut(s) 25, 29, 57, 199, 220, 534
FalI AAGNNNNNCTT 2 cut(s) 43, 75
FatI CATG 2 cut(s) 27, 218
FauI CCCGC 1 cut(s) 468
Fnu4HI GCNGC 6 cut(s) 146, 495, 498, 609, 612, 636
FokI GGATG 2 cut(s) 79, 176
Fsp4HI GCNGC 6 cut(s) 146, 495, 498, 609, 612, 636
FspBI CTAG 2 cut(s) 393, 605
GluI GCNGC 6 cut(s) 146, 495, 498, 609, 612, 636
GsuI CTGGAG 1 cut(s) 268
HaeIII GGCC 2 cut(s) 116, 148
HapII CCGG 1 cut(s) 86
Hin1II CATG 2 cut(s) 31, 222
HindIII AAGCTT 1 cut(s) 560
HinfI GANTC 5 cut(s) 175, 228, 371, 377, 396
HpaII CCGG 1 cut(s) 86
Hpy166II GTNNAC 3 cut(s) 165, 215, 514
Hpy188I TCNGA 7 cut(s) 364, 376, 418, 430, 466, 544, 648
Hpy188III TCNNGA 1 cut(s) 247
Hpy8I GTNNAC 3 cut(s) 165, 215, 514
HpyAV CCTTC 4 cut(s) 106, 189, 196, 465
HpyCH4III ACNGT 3 cut(s) 294, 308, 358
HpyCH4IV ACGT 3 cut(s) 274, 421, 630
HpyCH4V TGCA 6 cut(s) 27, 165, 341, 494, 526, 614
HpyF10VI GCNNNNNNNGC 2 cut(s) 608, 657
HpyF3I CTNAG 2 cut(s) 415, 647
HpySE526I ACGT 3 cut(s) 274, 421, 630
Hsp92II CATG 2 cut(s) 31, 222
Kzo9I GATC 3 cut(s) 31, 267, 622
Lsp1109I GCAGC 5 cut(s) 506, 509, 598, 620, 647
LweI GCATC 2 cut(s) 350, 513
MaeI CTAG 2 cut(s) 393, 605
MaeII ACGT 3 cut(s) 274, 421, 630
MaeIII GTNAC 1 cut(s) 308
MalI GATC 3 cut(s) 33, 269, 624
MboI GATC 3 cut(s) 31, 267, 622
MboII GAAGA 3 cut(s) 342, 557, 560
MflI RGATCY 3 cut(s) 31, 267, 622
MhlI GDGCHC 1 cut(s) 167
MluCI AATT 7 cut(s) 13, 73, 108, 152, 382, 504, 642
MlyI GAGTC 1 cut(s) 390
MnlI CCTC 6 cut(s) 89, 106, 127, 130, 318, 576
Mph1103I ATGCAT 1 cut(s) 29
MseI TTAA 2 cut(s) 71, 627
MspA1I CMGCKG 2 cut(s) 611, 638
MspI CCGG 1 cut(s) 86
MspR9I CCNGG 2 cut(s) 87, 321
MvaI CCWGG 1 cut(s) 321
MwoI GCNNNNNNNGC 2 cut(s) 608, 657
NciI CCSGG 1 cut(s) 87
NdeII GATC 3 cut(s) 31, 267, 622
NlaIII CATG 2 cut(s) 31, 222
NlaIV GGNNCC 1 cut(s) 269
NmuCI GTSAC 1 cut(s) 308
NsiI ATGCAT 1 cut(s) 29
PceI AGGCCT 1 cut(s) 116
PfeI GAWTC 4 cut(s) 175, 228, 371, 377
PflMI CCANNNNNTGG 1 cut(s) 224
PfoI TCCNGGA 1 cut(s) 85
PkrI GCNGC 6 cut(s) 147, 496, 499, 610, 613, 637
PleI GAGTC 1 cut(s) 390
PpsI GAGTC 1 cut(s) 390
Ppu21I YACGTR 1 cut(s) 275
Psp6I CCWGG 1 cut(s) 319
PspGI CCWGG 1 cut(s) 319
PspN4I GGNNCC 1 cut(s) 269
PstI CTGCAG 1 cut(s) 616
PsuI RGATCY 3 cut(s) 31, 267, 622
PvuII CAGCTG 1 cut(s) 611
RsaI GTAC 5 cut(s) 214, 291, 355, 448, 513
RsaNI GTAC 5 cut(s) 213, 290, 354, 447, 512
SaqAI TTAA 2 cut(s) 71, 627
SatI GCNGC 6 cut(s) 146, 495, 498, 609, 612, 636
Sau3AI GATC 3 cut(s) 31, 267, 622
ScaI AGTACT 1 cut(s) 355
SchI GAGTC 1 cut(s) 390
ScrFI CCNGG 2 cut(s) 87, 321
SduI GDGCHC 1 cut(s) 167
SfaNI GCATC 2 cut(s) 350, 513
SfcI CTRYAG 1 cut(s) 612
Sse9I AATT 7 cut(s) 13, 73, 108, 152, 382, 504, 642
SseBI AGGCCT 1 cut(s) 116
SsiI CCGC 3 cut(s) 145, 475, 638
SspMI CTAG 2 cut(s) 393, 605
StuI AGGCCT 1 cut(s) 116
StyD4I CCNGG 2 cut(s) 85, 319
StyI CCWWGG 2 cut(s) 18, 208
TaaI ACNGT 3 cut(s) 294, 308, 358
TaiI ACGT 3 cut(s) 277, 424, 633
TaqI TCGA 1 cut(s) 349
TasI AATT 7 cut(s) 13, 73, 108, 152, 382, 504, 642
TatI WGTACW 3 cut(s) 289, 353, 511
TauI GCSGC 1 cut(s) 148
TfiI GAWTC 4 cut(s) 175, 228, 371, 377
Tru1I TTAA 2 cut(s) 71, 627
Tru9I TTAA 2 cut(s) 71, 627
TscAI CASTG 1 cut(s) 172
TseFI GTSAC 1 cut(s) 308
TseI GCWGC 5 cut(s) 494, 497, 608, 611, 635
Tsp45I GTSAC 1 cut(s) 308
TspDTI ATGAA 1 cut(s) 178
TspGWI ACGGA 1 cut(s) 252
TspRI CASTG 1 cut(s) 172
Van91I CCANNNNNTGG 1 cut(s) 224
VneI GTGCAC 1 cut(s) 163
XapI RAATTY 6 cut(s) 13, 73, 152, 382, 504, 642
XcmI CCANNNNNNNNNTGG 1 cut(s) 25
XspI CTAG 2 cut(s) 393, 605
ZrmI AGTACT 1 cut(s) 355
Zsp2I ATGCAT 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.