Rh3AG213200

Der1-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0007744
Physical Location & Seq
Reverse (-)
21515636 .. 21520833
5198 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG213200.1

Sequence Viewer

Length: 1215 bp
ATGAGGCCTAACATCGTTACCGAGGCAGGAGTGCAGACTAGGTTGGCACAATGGTGGGAGGGTCTTCCTTTCCTTACTTCTGCGGTGGTTGTTGTTTGCACAACTATTTACTTGGTTTGCCTCTTGGCCGGATATGACTCCTTCTATGAAATATGCTTTTTGCCGTCTGCAGTTATATCACGATTCCAAGTCTACAGGATTTTCACTGCCATTCTGTTTCATGGTTCGCTGCTTCATGTGTTGTTCAACATGATGGCTTTGGTTCCTTTGGGTTCTGAGTTGGAGAGAATTATGGGATCTGTCCGCTTGTTGTACATAATCATTCTGTTGGCTACAACCAATGCGCTGCTCCATCTTCTAATTGCACTTTTGGTGGCCTATAATCCTATTAAGCCTTTTGAGTATTTGATGCATGAGTGTGCAATAGGCTTCTCAGGAATCTTGTTTTCTATGATTGTAATAGAGACAAGTCTGAGTGGACAGCAATACAGAAGTGTGTTTGGGCTCTTTAATGTGCCTGCTAAATGGTATGCATGGATCTTACTGGTAGTGTTCCAACTTGTTATGTCTAATGTTTCATTACTGGGACACCTGTGTGGTATTCTGTCTGGCTTCGCATATAGTTACGGCTTGTTCAATTTCCTCATTCCTGGACCATCCTTCTATTCCAAAATTGAGGCCTCCTCGTTGCTTTCAACTTGTGTGAGGCGGCCTAAATTTATTTTGTGCACTGGTGGAAATCCCTCTGCCTTCATCCCTACGTATTCAAGCCAAGGTACCACGTCTAGTGGATTGTTTTCGGGAAATTTGTGGAGAAACTTATCTTCATGGATCCCACAGAGGGAAACATCTTCACAGGCAACACAAGACAGTCACATATTTCCTGGGAGGGGTAGGACACTAGGGTCCGCTCAAGGTCAGGCTGTTTCTGATGTTGATTCAGAATCAAACTTACAGGCTAGACTGTTGGATAATAGTGACTCAGAACATCCATCTGATGCAGGACGGCAGTTATCTGATGGAAGGCAAACAGTAAATAATACGGTGGCAGCTGGAGCTGAAATTCCTGTTCACCATCAGGATTATGTGGCTTCTGAAGAAGAAATACAAAAGCTTGTATCAATGGGTTTTGAAAGGACACAGGTTGAAGTTGCACTAGCAGCTGCAGATGGTGATATTAATGTGGCAGTCGAAATTCTGAGCCAACAGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid UBA

Protein Analysis

404

Amino Acids

44.3

Weight (kDa)

5.58

Isoelectric Point (pI)

45.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DER1 PF04511 22 - 209 1.8e-07 Der1-like family
Rhomboid PF01694 62 - 213 9e-27 Rhomboid domain
UBA PF00627 366 - 400 9.8e-10 UBA/TS-N domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 904
Acc65I GGTACC 1 cut(s) 776
AccB1I GGYRCC 1 cut(s) 776
AccBSI CCGCTC 1 cut(s) 911
AccI GTMKAC 1 cut(s) 192
AciI CCGC 4 cut(s) 83, 304, 709, 909
AclWI GGATC 4 cut(s) 304, 545, 826, 839
AcoI YGGCCR 1 cut(s) 126
AcsI RAATTY 4 cut(s) 716, 805, 1062, 1194
AcuI CTGAAG 1 cut(s) 1116
AfaI GTAC 2 cut(s) 314, 778
AfiI CCNNNNNNNGG 2 cut(s) 841, 890
AgsI TTSAA 6 cut(s) 247, 637, 696, 768, 1133, 1148
AjiI CACGTC 1 cut(s) 783
AjnI CCWGG 2 cut(s) 649, 883
AleI CACNNNNGTG 2 cut(s) 52, 594
AluBI AGCT 4 cut(s) 1052, 1058, 1114, 1163
AluI AGCT 4 cut(s) 1052, 1058, 1114, 1163
Alw21I GWGCWC 1 cut(s) 731
Alw26I GTCTC 1 cut(s) 458
Alw44I GTGCAC 1 cut(s) 727
AlwI GGATC 4 cut(s) 304, 545, 826, 839
AoxI GGCC 5 cut(s) 5, 126, 375, 678, 710
ApaLI GTGCAC 1 cut(s) 727
ApeKI GCWGC 5 cut(s) 229, 346, 1049, 1160, 1163
ApoI RAATTY 4 cut(s) 716, 805, 1062, 1194
AseI ATTAAT 1 cut(s) 1179
Asp718I GGTACC 1 cut(s) 776
AspLEI GCGC 1 cut(s) 346
AspS9I GGNCC 2 cut(s) 653, 906
AsuHPI GGTGA 2 cut(s) 1064, 1184
AvaII GGWCC 2 cut(s) 653, 906
BaeGI GKGCMC 1 cut(s) 731
BamHI GGATCC 1 cut(s) 831
BanI GGYRCC 1 cut(s) 776
BanII GRGCYC 1 cut(s) 507
BarI GAAGNNNNNNTAC 2 cut(s) 1089, 1121
BbsI GAAGAC 1 cut(s) 56
Bbv12I GWGCWC 1 cut(s) 731
BbvI GCAGC 5 cut(s) 216, 333, 1061, 1150, 1172
BccI CCATC 7 cut(s) 247, 360, 664, 1000, 1013, 1083, 1163
BceAI ACGGC 3 cut(s) 148, 643, 1022
BciT130I CCWGG 2 cut(s) 651, 885
BcoDI GTCTC 1 cut(s) 458
BfaI CTAG 5 cut(s) 39, 786, 902, 960, 1157
BfmI CTRYAG 3 cut(s) 168, 193, 1164
BisI GCNGC 6 cut(s) 230, 347, 710, 1050, 1161, 1164
BlsI GCNGC 6 cut(s) 231, 348, 711, 1051, 1162, 1165
Bme1390I CCNGG 2 cut(s) 651, 885
Bme18I GGWCC 2 cut(s) 653, 906
BmgBI CACGTC 1 cut(s) 783
BmgT120I GGNCC 2 cut(s) 653, 906
BmiI GGNNCC 4 cut(s) 264, 778, 833, 907
BmrFI CCNGG 2 cut(s) 651, 885
BmrI ACTGGG 1 cut(s) 593
BmsI GCATC 2 cut(s) 399, 988
BmuI ACTGGG 1 cut(s) 593
BpiI GAAGAC 1 cut(s) 56
BplI GAGNNNNNCTC 2 cut(s) 668, 700
BpmI CTGGAG 1 cut(s) 1074
BpuEI CTTGAG 1 cut(s) 897
BsaAI YACGTR 1 cut(s) 762
BsaJI CCNNGG 3 cut(s) 21, 772, 884
Bsc4I CCNNNNNNNGG 2 cut(s) 841, 890
Bse1I ACTGG 3 cut(s) 549, 588, 736
BseBI CCWGG 2 cut(s) 651, 885
BseDI CCNNGG 3 cut(s) 21, 772, 884
BseGI GGATG 3 cut(s) 656, 753, 988
BseLI CCNNNNNNNGG 2 cut(s) 841, 890
BseMII CTCAG 5 cut(s) 267, 447, 464, 996, 1190
BseNI ACTGG 3 cut(s) 549, 588, 736
BseRI GAGGAG 1 cut(s) 673
BseSI GKGCMC 1 cut(s) 731
BseXI GCAGC 5 cut(s) 216, 333, 1061, 1150, 1172
BsgI GTGCAG 1 cut(s) 53
BshFI GGCC 5 cut(s) 7, 128, 377, 680, 712
BshNI GGYRCC 1 cut(s) 776
BsiHKAI GWGCWC 1 cut(s) 731
BsiSI CCGG 1 cut(s) 129
BslFI GGGAC 1 cut(s) 600
BslI CCNNNNNNNGG 2 cut(s) 841, 890
BsmAI GTCTC 1 cut(s) 458
BsmFI GGGAC 1 cut(s) 600
BsnI GGCC 5 cut(s) 7, 128, 377, 680, 712
Bsp1286I GDGCHC 2 cut(s) 507, 731
Bsp1407I TGTACA 1 cut(s) 312
Bsp143I GATC 3 cut(s) 296, 537, 831
BspACI CCGC 4 cut(s) 83, 304, 709, 909
BspANI GGCC 5 cut(s) 7, 128, 377, 680, 712
BspCNI CTCAG 5 cut(s) 268, 446, 465, 995, 1191
BspLI GGNNCC 4 cut(s) 264, 778, 833, 907
BspMAI CTGCAG 2 cut(s) 172, 1168
BspPI GGATC 4 cut(s) 304, 545, 826, 839
BspT107I GGYRCC 1 cut(s) 776
BsrBI CCGCTC 1 cut(s) 911
BsrGI TGTACA 1 cut(s) 312
BsrI ACTGG 3 cut(s) 549, 588, 736
BssECI CCNNGG 3 cut(s) 21, 772, 884
BssMI GATC 3 cut(s) 296, 537, 831
BssT1I CCWWGG 1 cut(s) 772
Bst2UI CCWGG 2 cut(s) 651, 885
Bst4CI ACNGT 4 cut(s) 872, 966, 1033, 1045
BstAUI TGTACA 1 cut(s) 312
BstBAI YACGTR 1 cut(s) 762
BstC8I GCNNGC 1 cut(s) 519
BstDEI CTNAG 5 cut(s) 276, 433, 473, 982, 1199
BstF5I GGATG 3 cut(s) 656, 753, 988
BstHHI GCGC 1 cut(s) 346
BstKTI GATC 3 cut(s) 299, 540, 834
BstMAI GTCTC 1 cut(s) 458
BstMBI GATC 3 cut(s) 296, 537, 831
BstMWI GCNNNNNNNGC 2 cut(s) 1055, 1160
BstNI CCWGG 2 cut(s) 651, 885
BstSCI CCNGG 2 cut(s) 649, 883
BstSFI CTRYAG 3 cut(s) 168, 193, 1164
BstSLI GKGCMC 1 cut(s) 731
BstSNI TACGTA 1 cut(s) 762
BstV1I GCAGC 5 cut(s) 216, 333, 1061, 1150, 1172
BstV2I GAAGAC 1 cut(s) 56
BstX2I RGATCY 3 cut(s) 296, 537, 831
BstYI RGATCY 3 cut(s) 296, 537, 831
BsuRI GGCC 5 cut(s) 7, 128, 377, 680, 712
BtrI CACGTC 1 cut(s) 783
BtsCI GGATG 3 cut(s) 656, 753, 988
BtsI GCAGTG 1 cut(s) 204
BtsIMutI CAGTG 2 cut(s) 204, 729
Cac8I GCNNGC 1 cut(s) 519
CfoI GCGC 1 cut(s) 346
Cfr13I GGNCC 2 cut(s) 653, 906
Csp6I GTAC 2 cut(s) 313, 777
CviAII CATG 6 cut(s) 221, 236, 250, 413, 534, 828
CviQI GTAC 2 cut(s) 313, 777
DdeI CTNAG 5 cut(s) 276, 433, 473, 982, 1199
DpnI GATC 3 cut(s) 298, 539, 833
DpnII GATC 3 cut(s) 296, 537, 831
DrdI GACNNNNNNGTC 1 cut(s) 904
DseDI GACNNNNNNGTC 1 cut(s) 904
EaeI YGGCCR 1 cut(s) 126
Eco105I TACGTA 1 cut(s) 762
Eco130I CCWWGG 1 cut(s) 772
Eco147I AGGCCT 2 cut(s) 7, 680
Eco24I GRGCYC 1 cut(s) 507
Eco47I GGWCC 2 cut(s) 653, 906
Eco57I CTGAAG 1 cut(s) 1116
EcoRII CCWGG 2 cut(s) 649, 883
EcoT14I CCWWGG 1 cut(s) 772
EcoT22I ATGCAT 2 cut(s) 414, 535
EcoT38I GRGCYC 1 cut(s) 507
ErhI CCWWGG 1 cut(s) 772
FaeI CATG 6 cut(s) 224, 239, 253, 416, 537, 831
FaqI GGGAC 1 cut(s) 600
FatI CATG 6 cut(s) 220, 235, 249, 412, 533, 827
FblI GTMKAC 1 cut(s) 192
Fnu4HI GCNGC 6 cut(s) 230, 347, 710, 1050, 1161, 1164
FokI GGATG 3 cut(s) 643, 740, 975
FriOI GRGCYC 1 cut(s) 507
Fsp4HI GCNGC 6 cut(s) 230, 347, 710, 1050, 1161, 1164
FspBI CTAG 5 cut(s) 39, 786, 902, 960, 1157
GlaI GCGC 1 cut(s) 345
GluI GCNGC 6 cut(s) 230, 347, 710, 1050, 1161, 1164
GsuI CTGGAG 1 cut(s) 1074
HaeIII GGCC 5 cut(s) 7, 128, 377, 680, 712
HapII CCGG 1 cut(s) 129
HhaI GCGC 1 cut(s) 346
Hin1II CATG 6 cut(s) 224, 239, 253, 416, 537, 831
Hin6I GCGC 1 cut(s) 344
HinP1I GCGC 1 cut(s) 344
HindIII AAGCTT 1 cut(s) 1112
HinfI GANTC 6 cut(s) 137, 183, 438, 938, 944, 980
HpaII CCGG 1 cut(s) 129
HphI GGTGA 2 cut(s) 1064, 1184
Hpy166II GTNNAC 4 cut(s) 193, 479, 729, 1072
Hpy188I TCNGA 9 cut(s) 277, 474, 931, 943, 985, 997, 1018, 1096, 1200
Hpy188III TCNNGA 4 cut(s) 180, 435, 801, 1079
Hpy8I GTNNAC 4 cut(s) 193, 479, 729, 1072
HpyAV CCTTC 4 cut(s) 151, 670, 760, 1017
HpyCH4III ACNGT 4 cut(s) 872, 966, 1033, 1045
HpyCH4IV ACGT 2 cut(s) 761, 782
HpyF10VI GCNNNNNNNGC 2 cut(s) 1055, 1160
HpyF3I CTNAG 5 cut(s) 276, 433, 473, 982, 1199
HpySE526I ACGT 2 cut(s) 761, 782
Hsp92II CATG 6 cut(s) 224, 239, 253, 416, 537, 831
HspAI GCGC 1 cut(s) 344
KpnI GGTACC 1 cut(s) 780
Kzo9I GATC 3 cut(s) 296, 537, 831
LmnI GCTCC 2 cut(s) 354, 1055
Lsp1109I GCAGC 5 cut(s) 216, 333, 1061, 1150, 1172
LweI GCATC 2 cut(s) 399, 988
MaeI CTAG 5 cut(s) 39, 786, 902, 960, 1157
MaeII ACGT 2 cut(s) 761, 782
MaeIII GTNAC 4 cut(s) 16, 623, 872, 977
MalI GATC 3 cut(s) 298, 539, 833
MbiI CCGCTC 1 cut(s) 911
MboI GATC 3 cut(s) 296, 537, 831
MboII GAAGA 6 cut(s) 56, 347, 816, 843, 1109, 1112
MflI RGATCY 3 cut(s) 296, 537, 831
MhlI GDGCHC 2 cut(s) 507, 731
MluCI AATT 8 cut(s) 288, 360, 637, 672, 716, 805, 1062, 1194
MlyI GAGTC 2 cut(s) 131, 974
MmeI TCCRAC 3 cut(s) 261, 580, 948
Mph1103I ATGCAT 2 cut(s) 414, 535
MseI TTAA 4 cut(s) 390, 510, 1179, 1213
MslI CAYNNNNRTG 3 cut(s) 52, 417, 594
MspA1I CMGCKG 2 cut(s) 1052, 1163
MspI CCGG 1 cut(s) 129
MspR9I CCNGG 2 cut(s) 651, 885
MvaI CCWGG 2 cut(s) 651, 885
MwoI GCNNNNNNNGC 2 cut(s) 1055, 1160
NdeII GATC 3 cut(s) 296, 537, 831
NlaIII CATG 6 cut(s) 224, 239, 253, 416, 537, 831
NlaIV GGNNCC 4 cut(s) 264, 778, 833, 907
NmuCI GTSAC 2 cut(s) 872, 977
NsiI ATGCAT 2 cut(s) 414, 535
OliI CACNNNNGTG 2 cut(s) 52, 594
PceI AGGCCT 2 cut(s) 7, 680
PfeI GAWTC 4 cut(s) 183, 438, 938, 944
PfoI TCCNGGA 1 cut(s) 649
PkrI GCNGC 6 cut(s) 231, 348, 711, 1051, 1162, 1165
PleI GAGTC 2 cut(s) 131, 974
PpsI GAGTC 2 cut(s) 131, 974
Ppu21I YACGTR 1 cut(s) 762
PshBI ATTAAT 1 cut(s) 1179
Psp6I CCWGG 2 cut(s) 649, 883
PspGI CCWGG 2 cut(s) 649, 883
PspN4I GGNNCC 4 cut(s) 264, 778, 833, 907
PspPI GGNCC 2 cut(s) 653, 906
PstI CTGCAG 2 cut(s) 172, 1168
PsuI RGATCY 3 cut(s) 296, 537, 831
PvuII CAGCTG 2 cut(s) 1052, 1163
RsaI GTAC 2 cut(s) 314, 778
RsaNI GTAC 2 cut(s) 313, 777
RseI CAYNNNNRTG 3 cut(s) 52, 417, 594
SaqAI TTAA 4 cut(s) 390, 510, 1179, 1213
SatI GCNGC 6 cut(s) 230, 347, 710, 1050, 1161, 1164
Sau3AI GATC 3 cut(s) 296, 537, 831
Sau96I GGNCC 2 cut(s) 653, 906
SchI GAGTC 2 cut(s) 131, 974
ScrFI CCNGG 2 cut(s) 651, 885
SduI GDGCHC 2 cut(s) 507, 731
SfaNI GCATC 2 cut(s) 399, 988
SfcI CTRYAG 3 cut(s) 168, 193, 1164
SinI GGWCC 2 cut(s) 653, 906
SmiMI CAYNNNNRTG 3 cut(s) 52, 417, 594
SmlI CTYRAG 1 cut(s) 912
SmoI CTYRAG 1 cut(s) 912
SnaBI TACGTA 1 cut(s) 762
Sse9I AATT 8 cut(s) 288, 360, 637, 672, 716, 805, 1062, 1194
SseBI AGGCCT 2 cut(s) 7, 680
SsiI CCGC 4 cut(s) 83, 304, 709, 909
SspMI CTAG 5 cut(s) 39, 786, 902, 960, 1157
StuI AGGCCT 2 cut(s) 7, 680
StyD4I CCNGG 2 cut(s) 649, 883
StyI CCWWGG 1 cut(s) 772
TaaI ACNGT 4 cut(s) 872, 966, 1033, 1045
TaiI ACGT 2 cut(s) 764, 785
TaqI TCGA 1 cut(s) 1191
TasI AATT 8 cut(s) 288, 360, 637, 672, 716, 805, 1062, 1194
TatI WGTACW 1 cut(s) 312
TauI GCSGC 1 cut(s) 712
TfiI GAWTC 4 cut(s) 183, 438, 938, 944
Tru1I TTAA 4 cut(s) 390, 510, 1179, 1213
Tru9I TTAA 4 cut(s) 390, 510, 1179, 1213
TscAI CASTG 2 cut(s) 211, 736
TseFI GTSAC 2 cut(s) 872, 977
TseI GCWGC 5 cut(s) 229, 346, 1049, 1160, 1163
Tsp45I GTSAC 2 cut(s) 872, 977
TspDTI ATGAA 6 cut(s) 162, 209, 224, 567, 742, 816
TspRI CASTG 2 cut(s) 211, 736
VneI GTGCAC 1 cut(s) 727
VpaK11BI GGWCC 2 cut(s) 653, 906
VspI ATTAAT 1 cut(s) 1179
XapI RAATTY 4 cut(s) 716, 805, 1062, 1194
XmiI GTMKAC 1 cut(s) 192
XspI CTAG 5 cut(s) 39, 786, 902, 960, 1157
Zsp2I ATGCAT 2 cut(s) 414, 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.