RchiOBHm_Chr3g0477721

Der1-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0007744
Physical Location & Seq
Reverse (-)
23653130 .. 23659143
6014 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44301

Sequence Viewer

Length: 1212 bp
ATGAGGCCTAACATCGTTTCCGAGGCAGGAGTGCAGACTAGGTTGGCACAATGGTGGGAGGGTCTTCCTTTCCTTACTTCTGCGGTGGTTGTTGTTTGCACAACTATTTACTTGGTTTGCCTCTTGGCCGGATATGACTCCTTCTATGAAATATGCTTTTTGCCGTCTGCAGTTATATCACGATTCCAAGTCTACAGGATTTTCACTGCCATTCTGTTTCATGGTTCGCTGCTTCATGTGTTGTTCAACATGATGGCTTTGGTTCCTTTGGGTTCTGAGTTGGAGAGAATTATGGGATCTGTCCGCTTGTTGTACATAATCATTCTGTTGGCTACAACCAATGCTCTGCTCCATCTTCTAATTGCACTTTTGGTGGCCTATAATCCTATTAAGCCTTTTGAGTATTTGATGCATGAGTGTGCAATAGGCTTCTCAGGAATCTTGTTTTCTATGATTGTAATAGAGACAAGTCTGAGTGGACAGCAATACAGAAGTGTGTTTGGGCTCTTTAATGTGCCTGCTAAATGGTATGCATGGATCTTACTGGTAGTGTTCCAACTTGTTATGTCTAATGTTTCATTACTGGGACACCTGTGTGGTATTCTGTCTGGCTTCGCATATAGTTACGGCTTGTTCAATTTCCTCATTCCTGGACCATCCTTCTATTCCAAAATTGAGGCCTCCTCGTTGCTTTCAACTTGTGTGAGGCGGCCTAAATTTATTTTGTGCACTGGTGGAAATCCCTCTGCCTTCATCCCTACGTATTCAAGCCAAGGTACCACGTCTAGTGGATTGTTTTCGGCAAATTTGTGGAGAAACTTATCTTCATGGATCCCACAGAGGGAAACATCTTCACAGGCAACACAAGACGACATATTTCCTGGGAGGGGTAGGACACTAGGGTCCGCTCAAGGTCAGGCTGTTTCTGATGTTGATTCAGAGTCAAACTTACAGGCTAGACTGTTGGATAATAGTGACTCAGAACATCCATCTGATGCAGGACGGCAGTTATCTGATGGAAGGCAAACAGTAAATAATACGGTGGCAGCTGGAGCTGAAATTCCTGTTCACCATCAGGATTATGTGGCTTCTGAAGAAGAAATACAAAAGCTTGTATCAATGGGTTTTGAAAGGACACAGGTTGAAGTTGCACTAGCAGCTGCAGATGGTGATATTAATGTGGCAGTCGAAATTCTGAGCCAACAGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid UBA

Protein Analysis

403

Amino Acids

44.19

Weight (kDa)

5.37

Isoelectric Point (pI)

44.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DER1 PF04511 22 - 209 1.8e-07 Der1-like family
Rhomboid PF01694 62 - 213 9e-27 Rhomboid domain
UBA PF00627 365 - 399 9.8e-10 UBA/TS-N domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 901
Acc65I GGTACC 1 cut(s) 776
AccB1I GGYRCC 1 cut(s) 776
AccBSI CCGCTC 1 cut(s) 908
AccI GTMKAC 1 cut(s) 192
AciI CCGC 4 cut(s) 83, 304, 709, 906
AclWI GGATC 4 cut(s) 304, 545, 826, 839
AcoI YGGCCR 1 cut(s) 126
AcsI RAATTY 4 cut(s) 716, 805, 1059, 1191
AcuI CTGAAG 1 cut(s) 1113
AfaI GTAC 2 cut(s) 314, 778
AfiI CCNNNNNNNGG 2 cut(s) 841, 887
AgsI TTSAA 6 cut(s) 247, 637, 696, 768, 1130, 1145
AjiI CACGTC 1 cut(s) 783
AjnI CCWGG 2 cut(s) 649, 880
AleI CACNNNNGTG 2 cut(s) 52, 594
AluBI AGCT 4 cut(s) 1049, 1055, 1111, 1160
AluI AGCT 4 cut(s) 1049, 1055, 1111, 1160
Alw21I GWGCWC 1 cut(s) 731
Alw26I GTCTC 1 cut(s) 458
Alw44I GTGCAC 1 cut(s) 727
AlwI GGATC 4 cut(s) 304, 545, 826, 839
AoxI GGCC 5 cut(s) 5, 126, 375, 678, 710
ApaLI GTGCAC 1 cut(s) 727
ApeKI GCWGC 4 cut(s) 229, 1046, 1157, 1160
ApoI RAATTY 4 cut(s) 716, 805, 1059, 1191
AseI ATTAAT 1 cut(s) 1176
Asp718I GGTACC 1 cut(s) 776
AspS9I GGNCC 2 cut(s) 653, 903
AsuHPI GGTGA 2 cut(s) 1061, 1181
AvaII GGWCC 2 cut(s) 653, 903
BaeGI GKGCMC 1 cut(s) 731
BamHI GGATCC 1 cut(s) 831
BanI GGYRCC 1 cut(s) 776
BanII GRGCYC 1 cut(s) 507
BarI GAAGNNNNNNTAC 2 cut(s) 1086, 1118
BbsI GAAGAC 1 cut(s) 56
Bbv12I GWGCWC 1 cut(s) 731
BbvI GCAGC 4 cut(s) 216, 1058, 1147, 1169
BccI CCATC 7 cut(s) 247, 360, 664, 997, 1010, 1080, 1160
BceAI ACGGC 3 cut(s) 148, 643, 1019
BciT130I CCWGG 2 cut(s) 651, 882
BcoDI GTCTC 1 cut(s) 458
BfaI CTAG 5 cut(s) 39, 786, 899, 957, 1154
BfmI CTRYAG 3 cut(s) 168, 193, 1161
BisI GCNGC 5 cut(s) 230, 710, 1047, 1158, 1161
BlsI GCNGC 5 cut(s) 231, 711, 1048, 1159, 1162
Bme1390I CCNGG 2 cut(s) 651, 882
Bme18I GGWCC 2 cut(s) 653, 903
BmgBI CACGTC 1 cut(s) 783
BmgT120I GGNCC 2 cut(s) 653, 903
BmiI GGNNCC 4 cut(s) 264, 778, 833, 904
BmrFI CCNGG 2 cut(s) 651, 882
BmrI ACTGGG 1 cut(s) 593
BmsI GCATC 2 cut(s) 399, 985
BmuI ACTGGG 1 cut(s) 593
BpiI GAAGAC 1 cut(s) 56
BplI GAGNNNNNCTC 2 cut(s) 668, 700
BpmI CTGGAG 1 cut(s) 1071
BpuEI CTTGAG 1 cut(s) 894
BsaAI YACGTR 1 cut(s) 762
BsaJI CCNNGG 3 cut(s) 21, 772, 881
Bsc4I CCNNNNNNNGG 2 cut(s) 841, 887
Bse1I ACTGG 3 cut(s) 549, 588, 736
BseBI CCWGG 2 cut(s) 651, 882
BseDI CCNNGG 3 cut(s) 21, 772, 881
BseGI GGATG 3 cut(s) 656, 753, 985
BseLI CCNNNNNNNGG 2 cut(s) 841, 887
BseMII CTCAG 5 cut(s) 267, 447, 464, 993, 1187
BseNI ACTGG 3 cut(s) 549, 588, 736
BseRI GAGGAG 1 cut(s) 673
BseSI GKGCMC 1 cut(s) 731
BseXI GCAGC 4 cut(s) 216, 1058, 1147, 1169
BsgI GTGCAG 1 cut(s) 53
BshFI GGCC 5 cut(s) 7, 128, 377, 680, 712
BshNI GGYRCC 1 cut(s) 776
BsiHKAI GWGCWC 1 cut(s) 731
BsiSI CCGG 1 cut(s) 129
BslFI GGGAC 1 cut(s) 600
BslI CCNNNNNNNGG 2 cut(s) 841, 887
BsmAI GTCTC 1 cut(s) 458
BsmFI GGGAC 1 cut(s) 600
BsnI GGCC 5 cut(s) 7, 128, 377, 680, 712
Bsp1286I GDGCHC 2 cut(s) 507, 731
Bsp1407I TGTACA 1 cut(s) 312
Bsp143I GATC 3 cut(s) 296, 537, 831
BspACI CCGC 4 cut(s) 83, 304, 709, 906
BspANI GGCC 5 cut(s) 7, 128, 377, 680, 712
BspCNI CTCAG 5 cut(s) 268, 446, 465, 992, 1188
BspLI GGNNCC 4 cut(s) 264, 778, 833, 904
BspMAI CTGCAG 2 cut(s) 172, 1165
BspPI GGATC 4 cut(s) 304, 545, 826, 839
BspT107I GGYRCC 1 cut(s) 776
BsrBI CCGCTC 1 cut(s) 908
BsrGI TGTACA 1 cut(s) 312
BsrI ACTGG 3 cut(s) 549, 588, 736
BssECI CCNNGG 3 cut(s) 21, 772, 881
BssMI GATC 3 cut(s) 296, 537, 831
BssT1I CCWWGG 1 cut(s) 772
Bst2UI CCWGG 2 cut(s) 651, 882
Bst4CI ACNGT 3 cut(s) 963, 1030, 1042
BstAUI TGTACA 1 cut(s) 312
BstBAI YACGTR 1 cut(s) 762
BstC8I GCNNGC 1 cut(s) 519
BstDEI CTNAG 5 cut(s) 276, 433, 473, 979, 1196
BstF5I GGATG 3 cut(s) 656, 753, 985
BstKTI GATC 3 cut(s) 299, 540, 834
BstMAI GTCTC 1 cut(s) 458
BstMBI GATC 3 cut(s) 296, 537, 831
BstMWI GCNNNNNNNGC 2 cut(s) 1052, 1157
BstNI CCWGG 2 cut(s) 651, 882
BstSCI CCNGG 2 cut(s) 649, 880
BstSFI CTRYAG 3 cut(s) 168, 193, 1161
BstSLI GKGCMC 1 cut(s) 731
BstSNI TACGTA 1 cut(s) 762
BstV1I GCAGC 4 cut(s) 216, 1058, 1147, 1169
BstV2I GAAGAC 1 cut(s) 56
BstX2I RGATCY 3 cut(s) 296, 537, 831
BstYI RGATCY 3 cut(s) 296, 537, 831
BsuRI GGCC 5 cut(s) 7, 128, 377, 680, 712
BtrI CACGTC 1 cut(s) 783
BtsCI GGATG 3 cut(s) 656, 753, 985
BtsI GCAGTG 1 cut(s) 204
BtsIMutI CAGTG 2 cut(s) 204, 729
Cac8I GCNNGC 1 cut(s) 519
Cfr13I GGNCC 2 cut(s) 653, 903
Csp6I GTAC 2 cut(s) 313, 777
CviAII CATG 6 cut(s) 221, 236, 250, 413, 534, 828
CviQI GTAC 2 cut(s) 313, 777
DdeI CTNAG 5 cut(s) 276, 433, 473, 979, 1196
DpnI GATC 3 cut(s) 298, 539, 833
DpnII GATC 3 cut(s) 296, 537, 831
DrdI GACNNNNNNGTC 1 cut(s) 901
DseDI GACNNNNNNGTC 1 cut(s) 901
EaeI YGGCCR 1 cut(s) 126
Eco105I TACGTA 1 cut(s) 762
Eco130I CCWWGG 1 cut(s) 772
Eco147I AGGCCT 2 cut(s) 7, 680
Eco24I GRGCYC 1 cut(s) 507
Eco47I GGWCC 2 cut(s) 653, 903
Eco57I CTGAAG 1 cut(s) 1113
EcoRII CCWGG 2 cut(s) 649, 880
EcoT14I CCWWGG 1 cut(s) 772
EcoT22I ATGCAT 2 cut(s) 414, 535
EcoT38I GRGCYC 1 cut(s) 507
ErhI CCWWGG 1 cut(s) 772
FaeI CATG 6 cut(s) 224, 239, 253, 416, 537, 831
FaqI GGGAC 1 cut(s) 600
FatI CATG 6 cut(s) 220, 235, 249, 412, 533, 827
FblI GTMKAC 1 cut(s) 192
Fnu4HI GCNGC 5 cut(s) 230, 710, 1047, 1158, 1161
FokI GGATG 3 cut(s) 643, 740, 972
FriOI GRGCYC 1 cut(s) 507
Fsp4HI GCNGC 5 cut(s) 230, 710, 1047, 1158, 1161
FspBI CTAG 5 cut(s) 39, 786, 899, 957, 1154
GluI GCNGC 5 cut(s) 230, 710, 1047, 1158, 1161
GsuI CTGGAG 1 cut(s) 1071
HaeIII GGCC 5 cut(s) 7, 128, 377, 680, 712
HapII CCGG 1 cut(s) 129
Hin1II CATG 6 cut(s) 224, 239, 253, 416, 537, 831
HindIII AAGCTT 1 cut(s) 1109
HinfI GANTC 6 cut(s) 137, 183, 438, 935, 941, 977
HpaII CCGG 1 cut(s) 129
HphI GGTGA 2 cut(s) 1061, 1181
Hpy166II GTNNAC 4 cut(s) 193, 479, 729, 1069
Hpy188III TCNNGA 3 cut(s) 180, 435, 1076
Hpy8I GTNNAC 4 cut(s) 193, 479, 729, 1069
HpyAV CCTTC 4 cut(s) 151, 670, 760, 1014
HpyCH4III ACNGT 3 cut(s) 963, 1030, 1042
HpyCH4IV ACGT 2 cut(s) 761, 782
HpyF10VI GCNNNNNNNGC 2 cut(s) 1052, 1157
HpyF3I CTNAG 5 cut(s) 276, 433, 473, 979, 1196
HpySE526I ACGT 2 cut(s) 761, 782
Hsp92II CATG 6 cut(s) 224, 239, 253, 416, 537, 831
KpnI GGTACC 1 cut(s) 780
Kzo9I GATC 3 cut(s) 296, 537, 831
LmnI GCTCC 2 cut(s) 354, 1052
Lsp1109I GCAGC 4 cut(s) 216, 1058, 1147, 1169
LweI GCATC 2 cut(s) 399, 985
MaeI CTAG 5 cut(s) 39, 786, 899, 957, 1154
MaeII ACGT 2 cut(s) 761, 782
MaeIII GTNAC 2 cut(s) 623, 974
MalI GATC 3 cut(s) 298, 539, 833
MbiI CCGCTC 1 cut(s) 908
MboI GATC 3 cut(s) 296, 537, 831
MboII GAAGA 6 cut(s) 56, 347, 816, 843, 1106, 1109
MflI RGATCY 3 cut(s) 296, 537, 831
MhlI GDGCHC 2 cut(s) 507, 731
MluCI AATT 8 cut(s) 288, 360, 637, 672, 716, 805, 1059, 1191
MlyI GAGTC 3 cut(s) 131, 950, 971
MmeI TCCRAC 3 cut(s) 261, 580, 945
Mph1103I ATGCAT 2 cut(s) 414, 535
MseI TTAA 4 cut(s) 390, 510, 1176, 1210
MslI CAYNNNNRTG 3 cut(s) 52, 417, 594
MspA1I CMGCKG 2 cut(s) 1049, 1160
MspI CCGG 1 cut(s) 129
MspR9I CCNGG 2 cut(s) 651, 882
MvaI CCWGG 2 cut(s) 651, 882
MwoI GCNNNNNNNGC 2 cut(s) 1052, 1157
NdeII GATC 3 cut(s) 296, 537, 831
NlaIII CATG 6 cut(s) 224, 239, 253, 416, 537, 831
NlaIV GGNNCC 4 cut(s) 264, 778, 833, 904
NmuCI GTSAC 1 cut(s) 974
NsiI ATGCAT 2 cut(s) 414, 535
OliI CACNNNNGTG 2 cut(s) 52, 594
PceI AGGCCT 2 cut(s) 7, 680
PfeI GAWTC 3 cut(s) 183, 438, 935
PfoI TCCNGGA 1 cut(s) 649
PkrI GCNGC 5 cut(s) 231, 711, 1048, 1159, 1162
PleI GAGTC 3 cut(s) 131, 949, 971
PpsI GAGTC 3 cut(s) 131, 949, 971
Ppu21I YACGTR 1 cut(s) 762
PshBI ATTAAT 1 cut(s) 1176
Psp6I CCWGG 2 cut(s) 649, 880
PspGI CCWGG 2 cut(s) 649, 880
PspN4I GGNNCC 4 cut(s) 264, 778, 833, 904
PspPI GGNCC 2 cut(s) 653, 903
PstI CTGCAG 2 cut(s) 172, 1165
PsuI RGATCY 3 cut(s) 296, 537, 831
PvuII CAGCTG 2 cut(s) 1049, 1160
RsaI GTAC 2 cut(s) 314, 778
RsaNI GTAC 2 cut(s) 313, 777
RseI CAYNNNNRTG 3 cut(s) 52, 417, 594
SaqAI TTAA 4 cut(s) 390, 510, 1176, 1210
SatI GCNGC 5 cut(s) 230, 710, 1047, 1158, 1161
Sau3AI GATC 3 cut(s) 296, 537, 831
Sau96I GGNCC 2 cut(s) 653, 903
SchI GAGTC 3 cut(s) 131, 950, 971
ScrFI CCNGG 2 cut(s) 651, 882
SduI GDGCHC 2 cut(s) 507, 731
SfaNI GCATC 2 cut(s) 399, 985
SfcI CTRYAG 3 cut(s) 168, 193, 1161
SinI GGWCC 2 cut(s) 653, 903
SmiMI CAYNNNNRTG 3 cut(s) 52, 417, 594
SmlI CTYRAG 1 cut(s) 909
SmoI CTYRAG 1 cut(s) 909
SnaBI TACGTA 1 cut(s) 762
Sse9I AATT 8 cut(s) 288, 360, 637, 672, 716, 805, 1059, 1191
SseBI AGGCCT 2 cut(s) 7, 680
SsiI CCGC 4 cut(s) 83, 304, 709, 906
SspMI CTAG 5 cut(s) 39, 786, 899, 957, 1154
StuI AGGCCT 2 cut(s) 7, 680
StyD4I CCNGG 2 cut(s) 649, 880
StyI CCWWGG 1 cut(s) 772
TaaI ACNGT 3 cut(s) 963, 1030, 1042
TaiI ACGT 2 cut(s) 764, 785
TaqI TCGA 1 cut(s) 1188
TasI AATT 8 cut(s) 288, 360, 637, 672, 716, 805, 1059, 1191
TatI WGTACW 1 cut(s) 312
TauI GCSGC 1 cut(s) 712
TfiI GAWTC 3 cut(s) 183, 438, 935
Tru1I TTAA 4 cut(s) 390, 510, 1176, 1210
Tru9I TTAA 4 cut(s) 390, 510, 1176, 1210
TscAI CASTG 2 cut(s) 211, 736
TseFI GTSAC 1 cut(s) 974
TseI GCWGC 4 cut(s) 229, 1046, 1157, 1160
Tsp45I GTSAC 1 cut(s) 974
TspDTI ATGAA 6 cut(s) 162, 209, 224, 567, 742, 816
TspRI CASTG 2 cut(s) 211, 736
VneI GTGCAC 1 cut(s) 727
VpaK11BI GGWCC 2 cut(s) 653, 903
VspI ATTAAT 1 cut(s) 1176
XapI RAATTY 4 cut(s) 716, 805, 1059, 1191
XmiI GTMKAC 1 cut(s) 192
XspI CTAG 5 cut(s) 39, 786, 899, 957, 1154
Zsp2I ATGCAT 2 cut(s) 414, 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.