Rroxscaffold_6G00403720

Der1-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0007744
Physical Location & Seq
Forward (+)
26192926 .. 26198586
5661 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00403720.1

Sequence Viewer

Length: 1314 bp
ATGAGGCCTAACATCGTTTCCGAGGCAGGAGTGCAAACTAGGTTGGCACAATGGTGGGAGGGTCTTCCTTTCCTTACTTCTGTGGTGGTTGTTGTTTGCACAATTATTTACTTGGTTTGCCTCTTGGCTGGATACGACTCCTTCTATGAAATATGCTTTTTGCCGTCTGCAGTTATCTCACGATTCCAAGTGTACAGGATTTTCACTGCCATTCTGTTTCATGGTTCACTGCTTCATGTGTTGTTCAACATGATGGCATTGGTTCCTTTGGGTTCTGAGTTGGAGAGAATTATGGGATCTGTCCGCTTGTTGTACATAATCATTCTGTTGGCTACAACCAATGCGCTGCTCCATCTTATGATTGCACTTTTGGTGGCCTATAATCCTATTAAGCCTTTTGACTATTTGATGCATGAGTGTGCAATAGGCTTCTCAGGAATCTTGTTTTCTATGATTGTAATAGAGACAAGTCTGAGTGGACAGCAATACAGAAGTGTGTTTGGGCTCTTTAATGTGCCTGCTAAATGGTATGCATGGATCTTACTGGTAGTGTTCCAACTTGTTATGTCTAATGTTTCATTACTGGGACACCTGTGTGGTATTCTGTCTGGCTTCGCATATAGTTATGGCTTGTTCAATTTCCTCATTCCTGGACCATCCTTCTATTCCAAAATTGAGGCCTCCTCGTTGCTTTCAACTTGTGTGAGGCGGCCCAAATTTATTTTGTGCACTGGTGGAAATCCCTCTGCCTTCATCCCTACGTATTCAAGCCAAGATCTCATTGTGTATTATAAGAGTCGTAATATGATATACTTTCTTAACCAGTGGATTATTTTCGGGAAATTTGTGGAGAAACTTATCTTCATGGATCCCACAGAGGGAAACATCTTCACAGGCCATTTGGAATATGACACTGTTCTTGATTTCATATGCATTAGTAGTATATTTTGTGTGCAGGCAACACAAGACAGTCACATATTTCCTGGGAGGGGTAGGACACTGGGGTCCGCTCAAGGTCAGGCTGTTTCTGATGTTGATTCAGAATCAAACTTACAGGCTAGACTGTTGGATAATAGTGACTCAGAACATCCATCTGATGCAGGACGGCAGTTATCTGATGGAAGGCAAACAGTAAATAATATGGTGGCAGCTGGAGCTGAAATTCCTGTTCACCATCAGGATTATGTGGCTTCTGAAGAAGAAATAGAAAAGCTTGTATCAATGGGTTTTGAAAGGACACAGGTTGAAGTTGCACTAGCAGCTGCAGATGGTGATATTAATGTGGCAGTTGAAATTCTGAGCCAACAGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid UBA

Protein Analysis

437

Amino Acids

48.56

Weight (kDa)

5.18

Isoelectric Point (pI)

43.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DER1 PF04511 22 - 209 1.5e-07 Der1-like family
Rhomboid PF01694 62 - 213 2e-26 Rhomboid domain
UBA PF00627 399 - 433 1.9e-09 UBA/TS-N domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 792
AasI GACNNNNNNGTC 1 cut(s) 1003
AccBSI CCGCTC 1 cut(s) 1010
AciI CCGC 3 cut(s) 304, 709, 1008
AclWI GGATC 4 cut(s) 304, 545, 863, 876
AcsI RAATTY 4 cut(s) 716, 842, 1161, 1293
AcuI CTGAAG 1 cut(s) 1215
AfaI GTAC 2 cut(s) 194, 314
AfiI CCNNNNNNNGG 2 cut(s) 878, 989
AgsI TTSAA 7 cut(s) 247, 637, 696, 768, 1232, 1247, 1292
AjnI CCWGG 2 cut(s) 649, 982
AleI CACNNNNGTG 2 cut(s) 52, 594
AluBI AGCT 4 cut(s) 1151, 1157, 1213, 1262
AluI AGCT 4 cut(s) 1151, 1157, 1213, 1262
Alw21I GWGCWC 1 cut(s) 731
Alw26I GTCTC 1 cut(s) 458
Alw44I GTGCAC 1 cut(s) 727
AlwI GGATC 4 cut(s) 304, 545, 863, 876
AoxI GGCC 5 cut(s) 5, 375, 678, 710, 895
ApaLI GTGCAC 1 cut(s) 727
ApeKI GCWGC 4 cut(s) 346, 1148, 1259, 1262
ApoI RAATTY 4 cut(s) 716, 842, 1161, 1293
AseI ATTAAT 1 cut(s) 1278
AspLEI GCGC 1 cut(s) 346
AspS9I GGNCC 3 cut(s) 653, 711, 1005
AsuHPI GGTGA 2 cut(s) 1163, 1283
AvaII GGWCC 2 cut(s) 653, 1005
BaeGI GKGCMC 1 cut(s) 731
BamHI GGATCC 1 cut(s) 868
BanII GRGCYC 1 cut(s) 507
BbsI GAAGAC 1 cut(s) 56
Bbv12I GWGCWC 1 cut(s) 731
BbvI GCAGC 4 cut(s) 333, 1160, 1249, 1271
BccI CCATC 7 cut(s) 247, 360, 664, 1099, 1112, 1182, 1262
BceAI ACGGC 2 cut(s) 148, 1121
BciT130I CCWGG 2 cut(s) 651, 984
BciVI GTATCC 1 cut(s) 125
BcoDI GTCTC 1 cut(s) 458
BfaI CTAG 3 cut(s) 39, 1059, 1256
BfmI CTRYAG 2 cut(s) 168, 1263
BfuI GTATCC 1 cut(s) 125
BglII AGATCT 1 cut(s) 775
BisI GCNGC 5 cut(s) 347, 710, 1149, 1260, 1263
BlsI GCNGC 5 cut(s) 348, 711, 1150, 1261, 1264
Bme1390I CCNGG 2 cut(s) 651, 984
Bme18I GGWCC 2 cut(s) 653, 1005
BmgT120I GGNCC 3 cut(s) 653, 711, 1005
BmiI GGNNCC 3 cut(s) 264, 870, 1006
BmrFI CCNGG 2 cut(s) 651, 984
BmrI ACTGGG 2 cut(s) 593, 1010
BmsI GCATC 2 cut(s) 399, 1087
BmuI ACTGGG 2 cut(s) 593, 1010
BpiI GAAGAC 1 cut(s) 56
BplI GAGNNNNNCTC 2 cut(s) 668, 700
BpmI CTGGAG 1 cut(s) 1173
BpuEI CTTGAG 1 cut(s) 996
BsaAI YACGTR 1 cut(s) 762
BsaJI CCNNGG 2 cut(s) 21, 983
Bsc4I CCNNNNNNNGG 2 cut(s) 878, 989
Bse1I ACTGG 5 cut(s) 549, 588, 736, 823, 1005
BseBI CCWGG 2 cut(s) 651, 984
BseDI CCNNGG 2 cut(s) 21, 983
BseGI GGATG 3 cut(s) 656, 753, 1087
BseLI CCNNNNNNNGG 2 cut(s) 878, 989
BseMII CTCAG 5 cut(s) 267, 447, 464, 1095, 1289
BseNI ACTGG 5 cut(s) 549, 588, 736, 823, 1005
BseRI GAGGAG 1 cut(s) 673
BseSI GKGCMC 1 cut(s) 731
BseXI GCAGC 4 cut(s) 333, 1160, 1249, 1271
BsgI GTGCAG 1 cut(s) 974
BshFI GGCC 5 cut(s) 7, 377, 680, 712, 897
BsiHKAI GWGCWC 1 cut(s) 731
BslFI GGGAC 1 cut(s) 600
BslI CCNNNNNNNGG 2 cut(s) 878, 989
BsmAI GTCTC 1 cut(s) 458
BsmFI GGGAC 1 cut(s) 600
BsnI GGCC 5 cut(s) 7, 377, 680, 712, 897
Bsp1286I GDGCHC 2 cut(s) 507, 731
Bsp1407I TGTACA 2 cut(s) 192, 312
Bsp143I GATC 4 cut(s) 296, 537, 775, 868
BspACI CCGC 3 cut(s) 304, 709, 1008
BspANI GGCC 5 cut(s) 7, 377, 680, 712, 897
BspCNI CTCAG 5 cut(s) 268, 446, 465, 1094, 1290
BspLI GGNNCC 3 cut(s) 264, 870, 1006
BspMAI CTGCAG 2 cut(s) 172, 1267
BspPI GGATC 4 cut(s) 304, 545, 863, 876
BsrBI CCGCTC 1 cut(s) 1010
BsrGI TGTACA 2 cut(s) 192, 312
BsrI ACTGG 5 cut(s) 549, 588, 736, 823, 1005
BssECI CCNNGG 2 cut(s) 21, 983
BssMI GATC 4 cut(s) 296, 537, 775, 868
Bst2UI CCWGG 2 cut(s) 651, 984
Bst4CI ACNGT 4 cut(s) 916, 971, 1065, 1132
BstAUI TGTACA 2 cut(s) 192, 312
BstBAI YACGTR 1 cut(s) 762
BstC8I GCNNGC 2 cut(s) 519, 957
BstDEI CTNAG 5 cut(s) 276, 433, 473, 1081, 1298
BstF5I GGATG 3 cut(s) 656, 753, 1087
BstHHI GCGC 1 cut(s) 346
BstKTI GATC 4 cut(s) 299, 540, 778, 871
BstMAI GTCTC 1 cut(s) 458
BstMBI GATC 4 cut(s) 296, 537, 775, 868
BstMWI GCNNNNNNNGC 2 cut(s) 1154, 1259
BstNI CCWGG 2 cut(s) 651, 984
BstSCI CCNGG 2 cut(s) 649, 982
BstSFI CTRYAG 2 cut(s) 168, 1263
BstSLI GKGCMC 1 cut(s) 731
BstSNI TACGTA 1 cut(s) 762
BstV1I GCAGC 4 cut(s) 333, 1160, 1249, 1271
BstV2I GAAGAC 1 cut(s) 56
BstX2I RGATCY 4 cut(s) 296, 537, 775, 868
BstYI RGATCY 4 cut(s) 296, 537, 775, 868
BsuI GTATCC 1 cut(s) 125
BsuRI GGCC 5 cut(s) 7, 377, 680, 712, 897
BtsCI GGATG 3 cut(s) 656, 753, 1087
BtsI GCAGTG 2 cut(s) 204, 227
BtsIMutI CAGTG 6 cut(s) 204, 227, 729, 830, 912, 998
Cac8I GCNNGC 2 cut(s) 519, 957
CfoI GCGC 1 cut(s) 346
Cfr13I GGNCC 3 cut(s) 653, 711, 1005
Csp6I GTAC 2 cut(s) 193, 313
CviAII CATG 6 cut(s) 221, 236, 250, 413, 534, 865
CviQI GTAC 2 cut(s) 193, 313
DdeI CTNAG 5 cut(s) 276, 433, 473, 1081, 1298
DpnI GATC 4 cut(s) 298, 539, 777, 870
DpnII GATC 4 cut(s) 296, 537, 775, 868
DrdI GACNNNNNNGTC 1 cut(s) 1003
DseDI GACNNNNNNGTC 1 cut(s) 1003
Eco105I TACGTA 1 cut(s) 762
Eco147I AGGCCT 2 cut(s) 7, 680
Eco24I GRGCYC 1 cut(s) 507
Eco47I GGWCC 2 cut(s) 653, 1005
Eco57I CTGAAG 1 cut(s) 1215
EcoRII CCWGG 2 cut(s) 649, 982
EcoT22I ATGCAT 3 cut(s) 414, 535, 935
EcoT38I GRGCYC 1 cut(s) 507
FaeI CATG 6 cut(s) 224, 239, 253, 416, 537, 868
FaqI GGGAC 1 cut(s) 600
FatI CATG 6 cut(s) 220, 235, 249, 412, 533, 864
FauNDI CATATG 1 cut(s) 929
Fnu4HI GCNGC 5 cut(s) 347, 710, 1149, 1260, 1263
FokI GGATG 3 cut(s) 643, 740, 1074
FriOI GRGCYC 1 cut(s) 507
Fsp4HI GCNGC 5 cut(s) 347, 710, 1149, 1260, 1263
FspBI CTAG 3 cut(s) 39, 1059, 1256
GlaI GCGC 1 cut(s) 345
GluI GCNGC 5 cut(s) 347, 710, 1149, 1260, 1263
GsuI CTGGAG 1 cut(s) 1173
HaeIII GGCC 5 cut(s) 7, 377, 680, 712, 897
HhaI GCGC 1 cut(s) 346
Hin1II CATG 6 cut(s) 224, 239, 253, 416, 537, 868
Hin6I GCGC 1 cut(s) 344
HinP1I GCGC 1 cut(s) 344
HindIII AAGCTT 1 cut(s) 1211
HinfI GANTC 7 cut(s) 137, 183, 438, 796, 1037, 1043, 1079
HphI GGTGA 2 cut(s) 1163, 1283
Hpy166II GTNNAC 5 cut(s) 193, 227, 479, 729, 1171
Hpy188III TCNNGA 5 cut(s) 180, 435, 838, 920, 1178
Hpy8I GTNNAC 5 cut(s) 193, 227, 479, 729, 1171
HpyAV CCTTC 4 cut(s) 151, 670, 760, 1116
HpyCH4III ACNGT 4 cut(s) 916, 971, 1065, 1132
HpyCH4IV ACGT 1 cut(s) 761
HpyF10VI GCNNNNNNNGC 2 cut(s) 1154, 1259
HpyF3I CTNAG 5 cut(s) 276, 433, 473, 1081, 1298
HpySE526I ACGT 1 cut(s) 761
Hsp92II CATG 6 cut(s) 224, 239, 253, 416, 537, 868
HspAI GCGC 1 cut(s) 344
Kzo9I GATC 4 cut(s) 296, 537, 775, 868
LmnI GCTCC 2 cut(s) 354, 1154
Lsp1109I GCAGC 4 cut(s) 333, 1160, 1249, 1271
LweI GCATC 2 cut(s) 399, 1087
MaeI CTAG 3 cut(s) 39, 1059, 1256
MaeII ACGT 1 cut(s) 761
MaeIII GTNAC 2 cut(s) 971, 1076
MalI GATC 4 cut(s) 298, 539, 777, 870
MbiI CCGCTC 1 cut(s) 1010
MboI GATC 4 cut(s) 296, 537, 775, 868
MboII GAAGA 5 cut(s) 56, 853, 880, 1208, 1211
MflI RGATCY 4 cut(s) 296, 537, 775, 868
MhlI GDGCHC 2 cut(s) 507, 731
MluCI AATT 8 cut(s) 102, 288, 637, 672, 716, 842, 1161, 1293
MlyI GAGTC 3 cut(s) 131, 805, 1073
MmeI TCCRAC 3 cut(s) 261, 580, 1047
Mph1103I ATGCAT 3 cut(s) 414, 535, 935
MseI TTAA 5 cut(s) 390, 510, 819, 1278, 1312
MslI CAYNNNNRTG 3 cut(s) 52, 417, 594
MspA1I CMGCKG 2 cut(s) 1151, 1262
MspR9I CCNGG 2 cut(s) 651, 984
MvaI CCWGG 2 cut(s) 651, 984
MwoI GCNNNNNNNGC 2 cut(s) 1154, 1259
NdeI CATATG 1 cut(s) 929
NdeII GATC 4 cut(s) 296, 537, 775, 868
NlaIII CATG 6 cut(s) 224, 239, 253, 416, 537, 868
NlaIV GGNNCC 3 cut(s) 264, 870, 1006
NmuCI GTSAC 2 cut(s) 971, 1076
NsiI ATGCAT 3 cut(s) 414, 535, 935
OliI CACNNNNGTG 2 cut(s) 52, 594
PceI AGGCCT 2 cut(s) 7, 680
PfeI GAWTC 4 cut(s) 183, 438, 1037, 1043
PfoI TCCNGGA 1 cut(s) 649
PkrI GCNGC 5 cut(s) 348, 711, 1150, 1261, 1264
PleI GAGTC 3 cut(s) 131, 804, 1073
PpsI GAGTC 3 cut(s) 131, 804, 1073
Ppu21I YACGTR 1 cut(s) 762
PshBI ATTAAT 1 cut(s) 1278
PsiI TTATAA 1 cut(s) 792
Psp6I CCWGG 2 cut(s) 649, 982
PspGI CCWGG 2 cut(s) 649, 982
PspN4I GGNNCC 3 cut(s) 264, 870, 1006
PspPI GGNCC 3 cut(s) 653, 711, 1005
PstI CTGCAG 2 cut(s) 172, 1267
PsuI RGATCY 4 cut(s) 296, 537, 775, 868
PvuII CAGCTG 2 cut(s) 1151, 1262
RsaI GTAC 2 cut(s) 194, 314
RsaNI GTAC 2 cut(s) 193, 313
RseI CAYNNNNRTG 3 cut(s) 52, 417, 594
SaqAI TTAA 5 cut(s) 390, 510, 819, 1278, 1312
SatI GCNGC 5 cut(s) 347, 710, 1149, 1260, 1263
Sau3AI GATC 4 cut(s) 296, 537, 775, 868
Sau96I GGNCC 3 cut(s) 653, 711, 1005
SchI GAGTC 3 cut(s) 131, 805, 1073
ScrFI CCNGG 2 cut(s) 651, 984
SduI GDGCHC 2 cut(s) 507, 731
SetI ASST 9 cut(s) 44, 594, 764, 1018, 1153, 1159, 1215, 1245, 1264
SfaNI GCATC 2 cut(s) 399, 1087
SfcI CTRYAG 2 cut(s) 168, 1263
SinI GGWCC 2 cut(s) 653, 1005
SmiMI CAYNNNNRTG 3 cut(s) 52, 417, 594
SmlI CTYRAG 1 cut(s) 1011
SmoI CTYRAG 1 cut(s) 1011
SnaBI TACGTA 1 cut(s) 762
Sse9I AATT 8 cut(s) 102, 288, 637, 672, 716, 842, 1161, 1293
SseBI AGGCCT 2 cut(s) 7, 680
SsiI CCGC 3 cut(s) 304, 709, 1008
SspMI CTAG 3 cut(s) 39, 1059, 1256
StuI AGGCCT 2 cut(s) 7, 680
StyD4I CCNGG 2 cut(s) 649, 982
TaaI ACNGT 4 cut(s) 916, 971, 1065, 1132
TaiI ACGT 1 cut(s) 764
TasI AATT 8 cut(s) 102, 288, 637, 672, 716, 842, 1161, 1293
TatI WGTACW 2 cut(s) 192, 312
TauI GCSGC 1 cut(s) 712
TfiI GAWTC 4 cut(s) 183, 438, 1037, 1043
Tru1I TTAA 5 cut(s) 390, 510, 819, 1278, 1312
Tru9I TTAA 5 cut(s) 390, 510, 819, 1278, 1312
TscAI CASTG 6 cut(s) 211, 234, 736, 830, 919, 1005
TseFI GTSAC 2 cut(s) 971, 1076
TseI GCWGC 4 cut(s) 346, 1148, 1259, 1262
Tsp45I GTSAC 2 cut(s) 971, 1076
TspDTI ATGAA 7 cut(s) 162, 209, 224, 567, 742, 853, 916
TspRI CASTG 6 cut(s) 211, 234, 736, 830, 919, 1005
VneI GTGCAC 1 cut(s) 727
VpaK11BI GGWCC 2 cut(s) 653, 1005
VspI ATTAAT 1 cut(s) 1278
XapI RAATTY 4 cut(s) 716, 842, 1161, 1293
XspI CTAG 3 cut(s) 39, 1059, 1256
Zsp2I ATGCAT 3 cut(s) 414, 535, 935
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.