Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0007565

Physical Location & Seq

Genomic Coordinates Forward (+)

8335796 .. 8339671

Gene Structure 3876 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1072600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1596 bp

ATGGGTAATTGCTGTGCGACCCCCCAGACGGGTTCTCCGTTGAAGGACAAGAAGAACAGGAAGAATCCATTTTCGACAAACTACGTCGCCGGCCACGGAAATGGTGGTTCCAAGCTCTATGTGCTGAAAGACCCTACTGGCGCTGAAATCGAGCAGAGATACGAACTGGGTCGGGAGCTCGGCCGTGGAGAGTTCGGAATTACCTACCTTTGCACCGATAAGGACTCCGGCGAGAAGTTCGCCTGTAAGTCGATTTCGAAGAGCAAGCTGAGGACAGCTGTGGATATAGAGGATGTAACGAGAGAAGTTGAGATCATGAAGCATTTGCCTAAGCACCCCAATATAGTGAGCTTGAAAGACACCTATGAAGATGACCATGCTGTCCACATTGTTATGGAGCTCTGTGAGGGCGGTGAGCTCTTCGATCGAATCGTGTCCAGAGGCCACTACACAGAGCGTGCTGCTGCCGCCGTCACAAAGACGATTGTCGAAGTTGTTCAGATGTGTCACAAGCACGGTGTGATTCACCGGGATCTCAAACCCGAGAACTTCTTGTTTGAAAACAAGAAAGAAACAGCACCTTTAAAGGCAATTGATTTTGGGTTGTCCGTATTCTTTAAACCTGGTGAAGTATTTAACGAAATTGTTGGAAGTCCCTATTACATGGCTCCTGAGGTGCTTAAACGCAACTATGGTCCTGAAATTGACGTGTGGAGTGCTGGAGTTATACTCTATATCTTACTTTGTGGTGTTCCACCATTTTGGGCAGAAACTGAACAAGGAGTTGCAGAAGCAATTATACGGTCTGTTGTAAATTTTAAGAAGGACCCTTGGCCTCTGGTTTCTGATAATGCGAAAGACCTTGTGAAGAAGATGCTTAATCCTGATCCAAAGCGGAGGCTTACAGCTCAGGAAGTTCTAGATCATCCTTGGTTACAAAATGCAAAGAAAGCTCCAAATGTTTCTTTAGGTGAAACTGTTCGGTCAAGGCTCAAGCAATTCTCTGTTATGAACAAGCTCAAGAAAAGCGCTCTCAAGGTTATAGCTGAGCATTTGTCGATGGAGGAAGTTGGTGGTATACAGGAGGGATTTAAGCTCATGGATATTAACAACAAGGGTAAGATTAACATTGACGAGCTAAGAGTTGGGTTGCATAAACTTGGCCATCAAATTCCTGATGGGGATCTTCATATTCTGATGGAAGCTGGTGATGTAGATAATGATGGATATCTAGACTATGGAGAATTTGTAGCCATCTCTGTTCACCTAAGAAGGATGGGCAATGATGAGGAGCATCTTCGCAAAGCATTTGAATTCTTCGACAAAAACCGGAATGGGTATATTGAAATTGAAGAGCTGCGAGATGCCATGAGTAATGAAGTTGATGATAACATTGAAGATGTTATCAATGCCATTATTAATGACGTAGATACGGACAAGGATGGACGAATAAGTTATGAGGAGTTTGCCGCAATGATGAAGGCCGGGACAGATTGGAGGAAAGCATCAAGGCAGTATTCGCGAGATCGGTTCAATAGTCTCAGTTTGAAATTGTTTAGGGATGGATCATTGGAAGGTAAAACGGAGAGTACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0004697 GO:0004698 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009506 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0030054 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8 Pkinase

Protein Analysis

532

Amino Acids

59.94

Weight (kDa)

5.88

Isoelectric Point (pI)

37.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	54 - 312	5.5e-80	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	55 - 307	2.3e-27	Protein tyrosine and serine/threonine kinase
Kinase-like	PF14531	143 - 300	2.7e-06	Kinase-like
EF-hand_7	PF13499	360 - 418	1.6e-07	EF-hand domain pair
EF-hand_7	PF13499	430 - 494	7.7e-18	EF-hand domain pair
EF-hand_1	PF00036	433 - 458	2.7e-07	EF hand domain
EF-hand_6	PF13405	433 - 458	8.3e-07	EF-hand domain
EF-hand_1	PF00036	469 - 495	4.3e-07	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007565)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41860 AT2G41860 AT3G57530 AT3G57530
fragaria_vesca	FvH4_6g23540 FvH4_6g23540
malus_domestica	MD12G1072600.v1.1 MD14G1069700.v1.1
prunus_persica	Prupe.7G064300_v2.0.a1
pyrus_communis	pycom12g06130
rosa_chinensis	RchiOBHm_Chr3g0481541 RchiOBHm_Chr3g0481591
rosa_laevigata	RLG00000023387
rosa_multiflora	Rmu_sc0010846.1_g000001 Rmu_sc0017309.1_g000002 Rmu_sc0019423.1_g000002 Rmu_sc0039298.1_g000001
rosa_roxburghii	Rroxscaffold_6G00400340
rosa_rugosa	Rorug03G0192500
rosa_samantha	Rh3AG243800 Rh3BG279000 Rh3CG274000 Rh3DG270700
rosa_wichuraiana	Rw3G021780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	380
AccI	GTMKAC	1 cut(s)	1078
AccII	CGCG	1 cut(s)	1522
AciI	CCGC	4 cut(s)	411, 468, 895, 1470
AclWI	GGATC	4 cut(s)	540, 881, 1191, 1573
AcoI	YGGCCR	3 cut(s)	91, 181, 1162
AcsI	RAATTY	4 cut(s)	814, 1170, 1244, 1313
AdeI	CACNNNGTG	1 cut(s)	520
AfaI	GTAC	1 cut(s)	1591
AfeI	AGCGCT	1 cut(s)	1030
AfiI	CCNNNNNNNGG	2 cut(s)	28, 29
AflIII	ACRYGT	1 cut(s)	708
AgsI	TTSAA	9 cut(s)	43, 355, 560, 1313, 1346, 1352, 1397, 1534, 1549
AjiI	CACGTC	1 cut(s)	709
AjnI	CCWGG	1 cut(s)	622
Alw21I	GWGCWC	3 cut(s)	180, 402, 420
Alw26I	GTCTC	1 cut(s)	1544
AlwI	GGATC	4 cut(s)	540, 881, 1191, 1573
AlwNI	CAGNNNCTG	1 cut(s)	773
Ama87I	CYCGRG	1 cut(s)	542
Aor51HI	AGCGCT	1 cut(s)	1030
AoxI	GGCC	6 cut(s)	91, 181, 442, 833, 1162, 1482
ApeKI	GCWGC	3 cut(s)	461, 464, 1357
ApoI	RAATTY	4 cut(s)	814, 1170, 1244, 1313
AseI	ATTAAT	1 cut(s)	1419
Asp700I	GAANNNNTTC	2 cut(s)	495, 978
AspLEI	GCGC	2 cut(s)	143, 1031
AspS9I	GGNCC	2 cut(s)	695, 826
AsuC2I	CCSGG	2 cut(s)	530, 1486
AsuHPI	GGTGA	6 cut(s)	425, 518, 638, 983, 1220, 1256
AsuII	TTCGAA	1 cut(s)	257
AvaI	CYCGRG	1 cut(s)	542
AvaII	GGWCC	2 cut(s)	695, 826
AxyI	CCTNAGG	1 cut(s)	672
BalI	TGGCCA	1 cut(s)	1164
BanII	GRGCYC	3 cut(s)	180, 402, 420
BarI	GAAGNNNNNNTAC	2 cut(s)	783, 815
Bbv12I	GWGCWC	3 cut(s)	180, 402, 420
BbvCI	CCTCAGC	1 cut(s)	269
BbvI	GCAGC	3 cut(s)	448, 451, 1344
BccI	CCATC	9 cut(s)	1054, 1172, 1173, 1192, 1217, 1262, 1270, 1436, 1556
BceAI	ACGGC	2 cut(s)	168, 455
BciT130I	CCWGG	1 cut(s)	624
BcnI	CCSGG	2 cut(s)	530, 1486
BcoDI	GTCTC	1 cut(s)	1544
BfaI	CTAG	2 cut(s)	920, 1232
BfoI	RGCGCY	2 cut(s)	144, 1032
BisI	GCNGC	5 cut(s)	462, 465, 468, 1358, 1470
BlpI	GCTNAGC	1 cut(s)	1047
BlsI	GCNGC	5 cut(s)	463, 466, 469, 1359, 1471
Bme1390I	CCNGG	3 cut(s)	530, 624, 1486
Bme18I	GGWCC	2 cut(s)	695, 826
BmeT110I	CYCGRG	1 cut(s)	542
BmgBI	CACGTC	1 cut(s)	709
BmgT120I	GGNCC	2 cut(s)	695, 826
BmiI	GGNNCC	3 cut(s)	109, 669, 828
BmrFI	CCNGG	3 cut(s)	530, 624, 1486
BmrI	ACTGGG	1 cut(s)	176
BmsI	GCATC	4 cut(s)	864, 1303, 1354, 1514
BmuI	ACTGGG	1 cut(s)	176
BoxI	GACNNNNGTC	1 cut(s)	485
BplI	GAGNNNNNCTC	2 cut(s)	714, 746
BpmI	CTGGAG	1 cut(s)	741
Bpu10I	CCTNAGC	3 cut(s)	269, 330, 909
Bpu1102I	GCTNAGC	1 cut(s)	1047
Bpu14I	TTCGAA	1 cut(s)	257
BpuEI	CTTGAG	3 cut(s)	977, 1004, 1019
BpuMI	CCSGG	2 cut(s)	530, 1486
BsaBI	GATNNNNATC	2 cut(s)	1182, 1227
BsaJI	CCNNGG	4 cut(s)	94, 184, 830, 929
BsaWI	WCCGGW	1 cut(s)	1329
BsaXI	ACNNNNNCTCC	4 cut(s)	19, 49, 1233, 1263
Bsc4I	CCNNNNNNNGG	2 cut(s)	28, 29
Bse118I	RCCGGY	1 cut(s)	89
Bse1I	ACTGG	2 cut(s)	142, 171
Bse21I	CCTNAGG	1 cut(s)	672
Bse3DI	GCAATG	2 cut(s)	1288, 1479
Bse8I	GATNNNNATC	2 cut(s)	1182, 1227
BseBI	CCWGG	1 cut(s)	624
BseDI	CCNNGG	4 cut(s)	94, 184, 830, 929
BseGI	GGATG	5 cut(s)	298, 925, 1281, 1447, 1567
BseJI	GATNNNNATC	2 cut(s)	1182, 1227
BseLI	CCNNNNNNNGG	2 cut(s)	28, 29
BseMI	GCAATG	2 cut(s)	1288, 1479
BseMII	CTCAG	5 cut(s)	260, 663, 923, 1038, 1555
BseNI	ACTGG	2 cut(s)	142, 171
BseRI	GAGGAG	2 cut(s)	1304, 1475
BseX3I	CGGCCG	1 cut(s)	181
BseXI	GCAGC	3 cut(s)	448, 451, 1344
Bsh1236I	CGCG	1 cut(s)	1522
Bsh1285I	CGRYCG	2 cut(s)	184, 427
BshFI	GGCC	6 cut(s)	93, 183, 444, 835, 1164, 1484
BsiEI	CGRYCG	2 cut(s)	184, 427
BsiHKAI	GWGCWC	3 cut(s)	180, 402, 420
BsiHKCI	CYCGRG	1 cut(s)	542
BsiSI	CCGG	5 cut(s)	90, 228, 529, 1330, 1485
BslFI	GGGAC	2 cut(s)	639, 1501
BslI	CCNNNNNNNGG	2 cut(s)	28, 29
BsmAI	GTCTC	1 cut(s)	1544
BsmFI	GGGAC	2 cut(s)	639, 1501
BsnI	GGCC	6 cut(s)	93, 183, 444, 835, 1164, 1484
BsoBI	CYCGRG	1 cut(s)	542
Bsp119I	TTCGAA	1 cut(s)	257
Bsp1286I	GDGCHC	3 cut(s)	180, 402, 420
Bsp143I	GATC	8 cut(s)	312, 424, 532, 886, 922, 1183, 1525, 1565
Bsp1720I	GCTNAGC	1 cut(s)	1047
Bsp68I	TCGCGA	1 cut(s)	1522
BspACI	CCGC	4 cut(s)	411, 468, 895, 1470
BspANI	GGCC	6 cut(s)	93, 183, 444, 835, 1164, 1484
BspCNI	CTCAG	5 cut(s)	261, 664, 922, 1039, 1554
BspFNI	CGCG	1 cut(s)	1522
BspHI	TCATGA	1 cut(s)	315
BspLI	GGNNCC	3 cut(s)	109, 669, 828
BspPI	GGATC	4 cut(s)	540, 881, 1191, 1573
BspQI	GCTCTTC	3 cut(s)	254, 425, 1347
BspT104I	TTCGAA	1 cut(s)	257
BsrDI	GCAATG	2 cut(s)	1288, 1479
BsrFI	RCCGGY	1 cut(s)	89
BsrI	ACTGG	2 cut(s)	142, 171
BssAI	RCCGGY	1 cut(s)	89
BssECI	CCNNGG	4 cut(s)	94, 184, 830, 929
BssMI	GATC	8 cut(s)	312, 424, 532, 886, 922, 1183, 1525, 1565
BssNAI	GTATAC	1 cut(s)	1079
BssT1I	CCWWGG	2 cut(s)	830, 929
Bst1107I	GTATAC	1 cut(s)	1079
Bst2UI	CCWGG	1 cut(s)	624
Bst4CI	ACNGT	3 cut(s)	518, 804, 979
Bst6I	CTCTTC	3 cut(s)	254, 425, 1347
BstBI	TTCGAA	1 cut(s)	257
BstC8I	GCNNGC	3 cut(s)	91, 266, 459
BstDEI	CTNAG	8 cut(s)	269, 330, 672, 909, 1047, 1139, 1268, 1541
BstDSI	CCRYGG	2 cut(s)	94, 184
BstF5I	GGATG	5 cut(s)	298, 925, 1281, 1447, 1567
BstFNI	CGCG	1 cut(s)	1522
BstH2I	RGCGCY	2 cut(s)	144, 1032
BstHHI	GCGC	2 cut(s)	143, 1031
BstKTI	GATC	8 cut(s)	315, 427, 535, 889, 925, 1186, 1528, 1568
BstMAI	GTCTC	1 cut(s)	1544
BstMBI	GATC	8 cut(s)	312, 424, 532, 886, 922, 1183, 1525, 1565
BstMCI	CGRYCG	2 cut(s)	184, 427
BstMWI	GCNNNNNNNGC	4 cut(s)	121, 467, 950, 1519
BstNI	CCWGG	1 cut(s)	624
BstPAI	GACNNNNGTC	1 cut(s)	485
BstSCI	CCNGG	3 cut(s)	528, 622, 1484
BstUI	CGCG	1 cut(s)	1522
BstV1I	GCAGC	3 cut(s)	448, 451, 1344
BstX2I	RGATCY	2 cut(s)	532, 1183
BstXI	CCANNNNNNTGG	2 cut(s)	101, 762
BstYI	RGATCY	2 cut(s)	532, 1183
BstZ17I	GTATAC	1 cut(s)	1079
BstZI	CGGCCG	1 cut(s)	181
Bsu36I	CCTNAGG	1 cut(s)	672
BsuRI	GGCC	6 cut(s)	93, 183, 444, 835, 1164, 1484
BtgI	CCRYGG	2 cut(s)	94, 184
BtrI	CACGTC	1 cut(s)	709
BtsCI	GGATG	5 cut(s)	298, 925, 1281, 1447, 1567
BtuMI	TCGCGA	1 cut(s)	1522
Cac8I	GCNNGC	3 cut(s)	91, 266, 459
CaiI	CAGNNNCTG	1 cut(s)	773
CciI	TCATGA	1 cut(s)	315
CfoI	GCGC	2 cut(s)	143, 1031
Cfr10I	RCCGGY	1 cut(s)	89
Cfr13I	GGNCC	2 cut(s)	695, 826
CsiI	ACCWGGT	1 cut(s)	622
Csp6I	GTAC	1 cut(s)	1590
CviAII	CATG	5 cut(s)	316, 377, 664, 1099, 1369
CviQI	GTAC	1 cut(s)	1590
DdeI	CTNAG	8 cut(s)	269, 330, 672, 909, 1047, 1139, 1268, 1541
DpnI	GATC	8 cut(s)	314, 426, 534, 888, 924, 1185, 1527, 1567
DpnII	GATC	8 cut(s)	312, 424, 532, 886, 922, 1183, 1525, 1565
DraI	TTTAAA	2 cut(s)	585, 619
DraIII	CACNNNGTG	1 cut(s)	520
DrdI	GACNNNNNNGTC	1 cut(s)	380
DseDI	GACNNNNNNGTC	1 cut(s)	380
EaeI	YGGCCR	3 cut(s)	91, 181, 1162
EagI	CGGCCG	1 cut(s)	181
Eam1104I	CTCTTC	3 cut(s)	254, 425, 1347
EarI	CTCTTC	3 cut(s)	254, 425, 1347
Ecl136II	GAGCTC	3 cut(s)	178, 400, 418
EclXI	CGGCCG	1 cut(s)	181
Eco130I	CCWWGG	2 cut(s)	830, 929
Eco24I	GRGCYC	3 cut(s)	180, 402, 420
Eco32I	GATATC	1 cut(s)	1229
Eco47I	GGWCC	2 cut(s)	695, 826
Eco47III	AGCGCT	1 cut(s)	1030
Eco52I	CGGCCG	1 cut(s)	181
Eco53kI	GAGCTC	3 cut(s)	178, 400, 418
Eco81I	CCTNAGG	1 cut(s)	672
Eco88I	CYCGRG	1 cut(s)	542
EcoICRI	GAGCTC	3 cut(s)	178, 400, 418
EcoO109I	RGGNCCY	1 cut(s)	826
EcoRI	GAATTC	1 cut(s)	1313
EcoRII	CCWGG	1 cut(s)	622
EcoRV	GATATC	1 cut(s)	1229
EcoT14I	CCWWGG	2 cut(s)	830, 929
EcoT38I	GRGCYC	3 cut(s)	180, 402, 420
ErhI	CCWWGG	2 cut(s)	830, 929
FaeI	CATG	5 cut(s)	319, 380, 667, 1102, 1372
FaqI	GGGAC	2 cut(s)	639, 1501
FatI	CATG	5 cut(s)	315, 376, 663, 1098, 1368
FblI	GTMKAC	1 cut(s)	1078
Fnu4HI	GCNGC	5 cut(s)	462, 465, 468, 1358, 1470
FokI	GGATG	5 cut(s)	305, 912, 1288, 1454, 1574
FriOI	GRGCYC	3 cut(s)	180, 402, 420
Fsp4HI	GCNGC	5 cut(s)	462, 465, 468, 1358, 1470
FspBI	CTAG	2 cut(s)	920, 1232
GlaI	GCGC	2 cut(s)	142, 1030
GluI	GCNGC	5 cut(s)	462, 465, 468, 1358, 1470
GsuI	CTGGAG	1 cut(s)	741
HaeII	RGCGCY	2 cut(s)	144, 1032
HaeIII	GGCC	6 cut(s)	93, 183, 444, 835, 1164, 1484
HapII	CCGG	5 cut(s)	90, 228, 529, 1330, 1485
HhaI	GCGC	2 cut(s)	143, 1031
Hin1II	CATG	5 cut(s)	319, 380, 667, 1102, 1372
Hin6I	GCGC	2 cut(s)	141, 1029
HinP1I	GCGC	2 cut(s)	141, 1029
HinfI	GANTC	4 cut(s)	64, 224, 429, 523
HpaII	CCGG	5 cut(s)	90, 228, 529, 1330, 1485
HphI	GGTGA	6 cut(s)	425, 518, 638, 983, 1220, 1256
Hpy166II	GTNNAC	3 cut(s)	385, 1079, 1264
Hpy188I	TCNGA	4 cut(s)	197, 501, 847, 1197
Hpy8I	GTNNAC	3 cut(s)	385, 1079, 1264
Hpy99I	CGWCG	1 cut(s)	89
HpyAV	CCTTC	5 cut(s)	37, 817, 1266, 1474, 1568
HpyCH4III	ACNGT	3 cut(s)	518, 804, 979
HpyCH4IV	ACGT	3 cut(s)	84, 708, 1425
HpyCH4V	TGCA	4 cut(s)	213, 788, 944, 1153
HpyF10VI	GCNNNNNNNGC	4 cut(s)	121, 467, 950, 1519
HpyF3I	CTNAG	8 cut(s)	269, 330, 672, 909, 1047, 1139, 1268, 1541
HpySE526I	ACGT	3 cut(s)	84, 708, 1425
Hsp92II	CATG	5 cut(s)	319, 380, 667, 1102, 1372
HspAI	GCGC	2 cut(s)	141, 1029
KroI	GCCGGC	1 cut(s)	89
KroNI	GCCGGC	1 cut(s)	91
Kzo9I	GATC	8 cut(s)	312, 424, 532, 886, 922, 1183, 1525, 1565
LguI	GCTCTTC	3 cut(s)	254, 425, 1347
LmnI	GCTCC	5 cut(s)	175, 397, 673, 958, 1291
Lsp1109I	GCAGC	3 cut(s)	448, 451, 1344
LweI	GCATC	4 cut(s)	864, 1303, 1354, 1514
MabI	ACCWGGT	1 cut(s)	622
MaeI	CTAG	2 cut(s)	920, 1232
MaeII	ACGT	3 cut(s)	84, 708, 1425
MaeIII	GTNAC	4 cut(s)	295, 472, 506, 933
MalI	GATC	8 cut(s)	314, 426, 534, 888, 924, 1185, 1527, 1567
MboI	GATC	8 cut(s)	312, 424, 532, 886, 922, 1183, 1525, 1565
MfeI	CAATTG	1 cut(s)	591
MflI	RGATCY	2 cut(s)	532, 1183
MhlI	GDGCHC	3 cut(s)	180, 402, 420
MlsI	TGGCCA	1 cut(s)	1164
MluNI	TGGCCA	1 cut(s)	1164
MlyI	GAGTC	1 cut(s)	218
MmeI	TCCRAC	1 cut(s)	628
Mox20I	TGGCCA	1 cut(s)	1164
MroNI	GCCGGC	1 cut(s)	89
MroXI	GAANNNNTTC	2 cut(s)	495, 978
MscI	TGGCCA	1 cut(s)	1164
MslI	CAYNNNNRTG	2 cut(s)	99, 392
Msp20I	TGGCCA	1 cut(s)	1164
MspA1I	CMGCKG	1 cut(s)	278
MspI	CCGG	5 cut(s)	90, 228, 529, 1330, 1485
MspR9I	CCNGG	3 cut(s)	530, 624, 1486
MunI	CAATTG	1 cut(s)	591
MvaI	CCWGG	1 cut(s)	624
MvnI	CGCG	1 cut(s)	1522
MwoI	GCNNNNNNNGC	4 cut(s)	121, 467, 950, 1519
NaeI	GCCGGC	1 cut(s)	91
NciI	CCSGG	2 cut(s)	530, 1486
NdeII	GATC	8 cut(s)	312, 424, 532, 886, 922, 1183, 1525, 1565
NgoMIV	GCCGGC	1 cut(s)	89
NlaIII	CATG	5 cut(s)	319, 380, 667, 1102, 1372
NlaIV	GGNNCC	3 cut(s)	109, 669, 828
NmeAIII	GCCGAG	1 cut(s)	159
NmuCI	GTSAC	2 cut(s)	472, 506
NruI	TCGCGA	1 cut(s)	1522
NspV	TTCGAA	1 cut(s)	257
PagI	TCATGA	1 cut(s)	315
PciSI	GCTCTTC	3 cut(s)	254, 425, 1347
PcsI	WCGNNNNNNNCGW	2 cut(s)	35, 429
PdiI	GCCGGC	1 cut(s)	91
PdmI	GAANNNNTTC	2 cut(s)	495, 978
PfeI	GAWTC	3 cut(s)	64, 429, 523
PkrI	GCNGC	5 cut(s)	463, 466, 469, 1359, 1471
Ple19I	CGATCG	1 cut(s)	427
PleI	GAGTC	1 cut(s)	218
PpsI	GAGTC	1 cut(s)	218
PpuMI	RGGWCCY	1 cut(s)	826
PshAI	GACNNNNGTC	1 cut(s)	485
PshBI	ATTAAT	1 cut(s)	1419
Psp124BI	GAGCTC	3 cut(s)	180, 402, 420
Psp5II	RGGWCCY	1 cut(s)	826
Psp6I	CCWGG	1 cut(s)	622
PspGI	CCWGG	1 cut(s)	622
PspN4I	GGNNCC	3 cut(s)	109, 669, 828
PspPI	GGNCC	2 cut(s)	695, 826
PspPPI	RGGWCCY	1 cut(s)	826
PstNI	CAGNNNCTG	1 cut(s)	773
PsuI	RGATCY	2 cut(s)	532, 1183
PvuI	CGATCG	1 cut(s)	427
PvuII	CAGCTG	1 cut(s)	278
RruI	TCGCGA	1 cut(s)	1522
RsaI	GTAC	1 cut(s)	1591
RsaNI	GTAC	1 cut(s)	1590
RseI	CAYNNNNRTG	2 cut(s)	99, 392
SacI	GAGCTC	3 cut(s)	180, 402, 420
SapI	GCTCTTC	3 cut(s)	254, 425, 1347
SatI	GCNGC	5 cut(s)	462, 465, 468, 1358, 1470
Sau3AI	GATC	8 cut(s)	312, 424, 532, 886, 922, 1183, 1525, 1565
Sau96I	GGNCC	2 cut(s)	695, 826
SchI	GAGTC	1 cut(s)	218
ScrFI	CCNGG	3 cut(s)	530, 624, 1486
SduI	GDGCHC	3 cut(s)	180, 402, 420
SexAI	ACCWGGT	1 cut(s)	622
SfaNI	GCATC	4 cut(s)	864, 1303, 1354, 1514
SfuI	TTCGAA	1 cut(s)	257
SinI	GGWCC	2 cut(s)	695, 826
SmiMI	CAYNNNNRTG	2 cut(s)	99, 392
SmlI	CTYRAG	3 cut(s)	992, 1019, 1034
SmoI	CTYRAG	3 cut(s)	992, 1019, 1034
SsiI	CCGC	4 cut(s)	411, 468, 895, 1470
SspMI	CTAG	2 cut(s)	920, 1232
SstI	GAGCTC	3 cut(s)	180, 402, 420
StyD4I	CCNGG	3 cut(s)	528, 622, 1484
StyI	CCWWGG	2 cut(s)	830, 929
TaaI	ACNGT	3 cut(s)	518, 804, 979
TaiI	ACGT	3 cut(s)	87, 711, 1428
TaqI	TCGA	9 cut(s)	74, 150, 251, 257, 423, 427, 489, 1058, 1320
TaqII	GACCGA	1 cut(s)	972
TatI	WGTACW	1 cut(s)	1589
TauI	GCSGC	2 cut(s)	470, 1472
TfiI	GAWTC	3 cut(s)	64, 429, 523
TseFI	GTSAC	2 cut(s)	472, 506
TseI	GCWGC	3 cut(s)	461, 464, 1357
Tsp45I	GTSAC	2 cut(s)	472, 506
TspDTI	ATGAA	6 cut(s)	332, 381, 1025, 1178, 1392, 1493
TspGWI	ACGGA	4 cut(s)	27, 111, 598, 1448
VpaK11BI	GGWCC	2 cut(s)	695, 826
VspI	ATTAAT	1 cut(s)	1419
XapI	RAATTY	4 cut(s)	814, 1170, 1244, 1313
XbaI	TCTAGA	2 cut(s)	919, 1231
XcmI	CCANNNNNNNNNTGG	1 cut(s)	101
XmiI	GTMKAC	1 cut(s)	1078
XmnI	GAANNNNTTC	2 cut(s)	495, 978
XspI	CTAG	2 cut(s)	920, 1232

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.