Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0007565

Physical Location & Seq

Genomic Coordinates Forward (+)

26423630 .. 26426729

Gene Structure 3100 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G021780.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1590 bp

ATGGGTAATTGCTGTGTGACCCCTCCCCAAACGGGTTCGCCCTTAAAGAACAAGAAGAACAAGCCGAACCCATTTGCCATAGACTACGCCGTCACCCATGGCGGCAGCAAGCTCTCCGTCTTGAAGGACCCAACTGGCACCGAAATCGAGCAGACGTATGAACTCGGCCGCGAGCTCGGCCGCGGAGAATTCGGAATCACCTACCTCTGTACTGACAAGGTCACCAGCGAGAAGTTCGCTTGCAAATCGATTTCGAAGCAGAAGCTGCGGACGGCTGTGGACATCGAGGATGTGAGGAGGGAGGTTGAGATCATGAAGCATTTGCCTAACCACCCCAATATTGTGAGCTTGAAAGATACGTATGAGGATGATAATGCGGTCCATCTCGTCATGGAGCTCTGTGAGGGCGGTGAGCTCTTCGATAGGATCGTCTCCAGGGGGCATTACACCGAGCGTGCCGCTGCCGCTGTCACTAAGACTATTGTCGAAGTTGTGCAGATGTGCCACAAGCACGGTGTGATGCATCGGGATCTCAAACCTGAGAACTTTTTATTTGCAAACAAGAAGGAAACAGCGGCTTTGAAGGCAATTGATTTCGGGTTGTCGGTCTTCTTTAAGCCTGGTGAAAGATTTAGTGAAATTGTTGGAAGTCCATACTACATGGCTCCCGAGGTGCTAAGGCGCAATTATGGTCCTGAAGTTGATGTGTGGAGTGCTGGAGTTATACTTTACATTTTACTTTGCGGTGTTCCACCTTTCTGGGCAGAAACTGAACAGGGAGTTGCACAAGCAATTATACGGTCTGTTGTAGATTTTAAGAGGGACCCCTGGCCAAAGGTTTCTGATAATGCAAAAGACCTTGTGAAGAAGATGCTTAATCCTGACCCGAAGCAGAGGCTTACAGCTCAGCAAGTTCTAGATCATACTTGGTTGCAAAATGCAAAGAAAGCTCCAAATGTTTCTTTAGGTGAAACAGTGAGAGCAAGGCTCAAGCAATTCTCTGTAATGAACAAGCTTAAGAAAAGTGCACTCAAGGTTATAGCTGAGCATTTGTCACAGGATGAAGTTGCTGGCATACAAGAGGGATTTAAGATCATGGATACTAGCAACAACGGCAAGATTAACATTGATGAGCTAAGAATTGGGCTACATAAACTTGGCCATCAGATTCCTGATACTGATCTTCATATCCTAATGGAAGCTGGTGATGTAGATAATGATGGGTATCTGGACTATGGAGAGTTTGTAGCCATTTCTGTTCACCTAAGAAGGATGGGCAATGATGATGAGCACCTTCGCAAAGCATTCGACTTCTTTGATCAAAACAAAAGTGGGTACATTGAAGTTGAGGAGTTGCGAACTGCCTTGGCTCCTGAAGTTGATGATAACGTTGAAGATGTTATTAGTGCCATTATCAATGACGTGGATACAGACAAGGATGGAAAAATAAGTTACGAGGAGTTTGCCGCCATGATGAAGGCCGGCACAGATTGGAGAAAAGCGTCAAGGCAGTATTCACGAGAGCGGTTCAACAGTCTGAGTTTGACATTGATGAGGGATGGATCGTTGGAGGGTAAAACTGAGAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0004697 GO:0004698 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009506 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0030054 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8 Pkinase

Protein Analysis

529

Amino Acids

59.48

Weight (kDa)

5.83

Isoelectric Point (pI)

35.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	53 - 311	1.3e-80	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	54 - 306	1.7e-27	Protein tyrosine and serine/threonine kinase
Kinase-like	PF14531	142 - 299	8.8e-06	Kinase-like
EF-hand_7	PF13499	359 - 417	2.9e-08	EF-hand domain pair
EF-hand_7	PF13499	430 - 493	5.5e-17	EF-hand domain pair
EF-hand_1	PF00036	432 - 456	4.2e-06	EF hand domain
EF-hand_6	PF13405	432 - 456	1.4e-06	EF-hand domain
EF-hand_1	PF00036	469 - 494	2.4e-07	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0007565)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41860 AT2G41860 AT3G57530 AT3G57530
fragaria_vesca	FvH4_6g23540 FvH4_6g23540
malus_domestica	MD12G1072600.v1.1 MD14G1069700.v1.1
prunus_persica	Prupe.7G064300_v2.0.a1
pyrus_communis	pycom12g06130
rosa_chinensis	RchiOBHm_Chr3g0481541 RchiOBHm_Chr3g0481591
rosa_laevigata	RLG00000023387
rosa_multiflora	Rmu_sc0010846.1_g000001 Rmu_sc0017309.1_g000002 Rmu_sc0019423.1_g000002 Rmu_sc0039298.1_g000001
rosa_roxburghii	Rroxscaffold_6G00400340
rosa_rugosa	Rorug03G0192500
rosa_samantha	Rh3AG243800 Rh3BG279000 Rh3CG274000 Rh3DG270700
rosa_wichuraiana	Rw3G021780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	89
AccB1I	GGYRCC	1 cut(s)	137
AccBSI	CCGCTC	1 cut(s)	1525
AccII	CGCG	2 cut(s)	171, 183
AclI	AACGTT	1 cut(s)	1389
AclWI	GGATC	3 cut(s)	434, 537, 1570
AcoI	YGGCCR	4 cut(s)	166, 178, 830, 1159
AcsI	RAATTY	1 cut(s)	188
AcuI	CTGAAG	2 cut(s)	717, 1395
AdeI	CACNNNGTG	1 cut(s)	517
AfaI	GTAC	2 cut(s)	211, 1337
AfiI	CCNNNNNNNGG	1 cut(s)	32
AflII	CTTAAG	1 cut(s)	1016
AgsI	TTSAA	6 cut(s)	124, 352, 583, 1343, 1394, 1531
AjiI	CACGTC	1 cut(s)	1423
AjnI	CCWGG	3 cut(s)	434, 619, 827
Alw21I	GWGCWC	5 cut(s)	177, 399, 417, 1030, 1293
Alw26I	GTCTC	1 cut(s)	436
Alw44I	GTGCAC	1 cut(s)	1026
AlwI	GGATC	3 cut(s)	434, 537, 1570
AlwNI	CAGNNNCTG	2 cut(s)	265, 770
Ama87I	CYCGRG	1 cut(s)	668
AoxI	GGCC	5 cut(s)	166, 178, 830, 1159, 1479
ApaLI	GTGCAC	1 cut(s)	1026
ApeKI	GCWGC	3 cut(s)	105, 265, 461
ApoI	RAATTY	1 cut(s)	188
AspLEI	GCGC	1 cut(s)	684
AspS9I	GGNCC	4 cut(s)	127, 379, 692, 823
AsuHPI	GGTGA	8 cut(s)	85, 190, 214, 422, 635, 980, 1217, 1253
AsuII	TTCGAA	1 cut(s)	254
AvaI	CYCGRG	1 cut(s)	668
AvaII	GGWCC	4 cut(s)	127, 379, 692, 823
BaeGI	GKGCMC	1 cut(s)	1030
BalI	TGGCCA	2 cut(s)	832, 1161
BanI	GGYRCC	1 cut(s)	137
BanII	GRGCYC	3 cut(s)	177, 399, 417
BauI	CACGAG	1 cut(s)	1518
BbsI	GAAGAC	1 cut(s)	601
Bbv12I	GWGCWC	5 cut(s)	177, 399, 417, 1030, 1293
BbvI	GCAGC	3 cut(s)	117, 252, 448
BccI	CCATC	6 cut(s)	390, 1170, 1214, 1267, 1433, 1553
BceAI	ACGGC	3 cut(s)	74, 288, 1129
BcgI	CGANNNNNNTGC	6 cut(s)	127, 161, 1288, 1322, 1445, 1479
BciT130I	CCWGG	3 cut(s)	436, 621, 829
BciVI	GTATCC	2 cut(s)	1093, 1420
BclI	TGATCA	1 cut(s)	1318
BcoDI	GTCTC	1 cut(s)	436
BfaI	CTAG	2 cut(s)	917, 1104
BfrI	CTTAAG	1 cut(s)	1016
BfuI	GTATCC	2 cut(s)	1093, 1420
BlpI	GCTNAGC	2 cut(s)	906, 1044
Bme1390I	CCNGG	3 cut(s)	436, 621, 829
Bme18I	GGWCC	4 cut(s)	127, 379, 692, 823
BmeT110I	CYCGRG	1 cut(s)	668
BmgBI	CACGTC	1 cut(s)	1423
BmgT120I	GGNCC	4 cut(s)	127, 379, 692, 823
BmiI	GGNNCC	6 cut(s)	129, 139, 666, 824, 825, 1371
BmrFI	CCNGG	3 cut(s)	436, 621, 829
BmsI	GCATC	3 cut(s)	510, 532, 861
BoxI	GACNNNNGTC	1 cut(s)	482
BpiI	GAAGAC	1 cut(s)	601
BplI	GAGNNNNNCTC	2 cut(s)	972, 1004
BpmI	CTGGAG	2 cut(s)	418, 738
Bpu10I	CCTNAGC	1 cut(s)	677
Bpu1102I	GCTNAGC	2 cut(s)	906, 1044
Bpu14I	TTCGAA	1 cut(s)	254
BpuEI	CTTGAG	2 cut(s)	974, 1016
Bsa29I	ATCGAT	1 cut(s)	248
BsaAI	YACGTR	1 cut(s)	360
BsaBI	GATNNNNATC	2 cut(s)	1179, 1224
BsaJI	CCNNGG	6 cut(s)	97, 181, 435, 669, 827, 1365
BsaXI	ACNNNNNCTCC	2 cut(s)	1230, 1260
Bsc4I	CCNNNNNNNGG	1 cut(s)	32
Bse118I	RCCGGY	1 cut(s)	1481
Bse1I	ACTGG	1 cut(s)	139
Bse3DI	GCAATG	1 cut(s)	1285
Bse8I	GATNNNNATC	2 cut(s)	1179, 1224
BseBI	CCWGG	3 cut(s)	436, 621, 829
BseCI	ATCGAT	1 cut(s)	248
BseDI	CCNNGG	6 cut(s)	97, 181, 435, 669, 827, 1365
BseGI	GGATG	6 cut(s)	295, 373, 1066, 1278, 1444, 1564
BseJI	GATNNNNATC	2 cut(s)	1179, 1224
BseLI	CCNNNNNNNGG	1 cut(s)	32
BseMI	GCAATG	1 cut(s)	1285
BseMII	CTCAG	5 cut(s)	531, 920, 1035, 1529, 1572
BseNI	ACTGG	1 cut(s)	139
BseRI	GAGGAG	3 cut(s)	310, 1364, 1472
BseSI	GKGCMC	1 cut(s)	1030
BseX3I	CGGCCG	2 cut(s)	166, 178
BseXI	GCAGC	3 cut(s)	117, 252, 448
BsgI	GTGCAG	1 cut(s)	515
Bsh1236I	CGCG	2 cut(s)	171, 183
Bsh1285I	CGRYCG	2 cut(s)	169, 181
BshFI	GGCC	5 cut(s)	168, 180, 832, 1161, 1481
BshNI	GGYRCC	1 cut(s)	137
BshVI	ATCGAT	1 cut(s)	248
BsiEI	CGRYCG	2 cut(s)	169, 181
BsiHKAI	GWGCWC	5 cut(s)	177, 399, 417, 1030, 1293
BsiHKCI	CYCGRG	1 cut(s)	668
BsiSI	CCGG	1 cut(s)	1482
BslFI	GGGAC	1 cut(s)	836
BslI	CCNNNNNNNGG	1 cut(s)	32
BsmAI	GTCTC	1 cut(s)	436
BsmBI	CGTCTC	1 cut(s)	436
BsmFI	GGGAC	1 cut(s)	836
BsmI	GAATGC	1 cut(s)	1304
BsnI	GGCC	5 cut(s)	168, 180, 832, 1161, 1481
BsoBI	CYCGRG	1 cut(s)	668
Bsp119I	TTCGAA	1 cut(s)	254
Bsp1286I	GDGCHC	5 cut(s)	177, 399, 417, 1030, 1293
Bsp143I	GATC	8 cut(s)	309, 426, 529, 919, 1092, 1180, 1318, 1562
Bsp1720I	GCTNAGC	2 cut(s)	906, 1044
Bsp19I	CCATGG	1 cut(s)	97
BspANI	GGCC	5 cut(s)	168, 180, 832, 1161, 1481
BspCNI	CTCAG	5 cut(s)	532, 919, 1036, 1530, 1573
BspDI	ATCGAT	1 cut(s)	248
BspFNI	CGCG	2 cut(s)	171, 183
BspHI	TCATGA	1 cut(s)	312
BspLI	GGNNCC	6 cut(s)	129, 139, 666, 824, 825, 1371
BspPI	GGATC	3 cut(s)	434, 537, 1570
BspQI	GCTCTTC	1 cut(s)	422
BspT104I	TTCGAA	1 cut(s)	254
BspT107I	GGYRCC	1 cut(s)	137
BspTI	CTTAAG	1 cut(s)	1016
BsrBI	CCGCTC	1 cut(s)	1525
BsrDI	GCAATG	1 cut(s)	1285
BsrFI	RCCGGY	1 cut(s)	1481
BsrI	ACTGG	1 cut(s)	139
BssAI	RCCGGY	1 cut(s)	1481
BssECI	CCNNGG	6 cut(s)	97, 181, 435, 669, 827, 1365
BssMI	GATC	8 cut(s)	309, 426, 529, 919, 1092, 1180, 1318, 1562
BssSI	CACGAG	1 cut(s)	1518
BssT1I	CCWWGG	2 cut(s)	97, 1365
Bst2BI	CACGAG	1 cut(s)	1518
Bst2UI	CCWGG	3 cut(s)	436, 621, 829
Bst4CI	ACNGT	4 cut(s)	515, 801, 976, 1535
Bst6I	CTCTTC	1 cut(s)	422
BstAFI	CTTAAG	1 cut(s)	1016
BstAPI	GCANNNNNTGC	1 cut(s)	265
BstBAI	YACGTR	1 cut(s)	360
BstBI	TTCGAA	1 cut(s)	254
BstC8I	GCNNGC	6 cut(s)	110, 173, 241, 456, 1072, 1483
BstDEI	CTNAG	9 cut(s)	474, 540, 677, 906, 1044, 1136, 1265, 1538, 1581
BstDSI	CCRYGG	2 cut(s)	97, 181
BstEII	GGTNACC	1 cut(s)	220
BstF5I	GGATG	6 cut(s)	295, 373, 1066, 1278, 1444, 1564
BstFNI	CGCG	2 cut(s)	171, 183
BstHHI	GCGC	1 cut(s)	684
BstKTI	GATC	8 cut(s)	312, 429, 532, 922, 1095, 1183, 1321, 1565
BstMAI	GTCTC	1 cut(s)	436
BstMBI	GATC	8 cut(s)	309, 426, 529, 919, 1092, 1180, 1318, 1562
BstMCI	CGRYCG	2 cut(s)	169, 181
BstMWI	GCNNNNNNNGC	6 cut(s)	177, 265, 464, 584, 947, 1113
BstNI	CCWGG	3 cut(s)	436, 621, 829
BstPAI	GACNNNNGTC	1 cut(s)	482
BstPI	GGTNACC	1 cut(s)	220
BstSCI	CCNGG	3 cut(s)	434, 619, 827
BstSLI	GKGCMC	1 cut(s)	1030
BstSNI	TACGTA	1 cut(s)	360
BstUI	CGCG	2 cut(s)	171, 183
BstV1I	GCAGC	3 cut(s)	117, 252, 448
BstV2I	GAAGAC	1 cut(s)	601
BstX2I	RGATCY	1 cut(s)	529
BstXI	CCANNNNNNTGG	1 cut(s)	759
BstYI	RGATCY	1 cut(s)	529
BstZI	CGGCCG	2 cut(s)	166, 178
Bsu15I	ATCGAT	1 cut(s)	248
BsuI	GTATCC	2 cut(s)	1093, 1420
BsuRI	GGCC	5 cut(s)	168, 180, 832, 1161, 1481
BsuTUI	ATCGAT	1 cut(s)	248
BtgI	CCRYGG	2 cut(s)	97, 181
BtrI	CACGTC	1 cut(s)	1423
BtsCI	GGATG	6 cut(s)	295, 373, 1066, 1278, 1444, 1564
BtsIMutI	CAGTG	1 cut(s)	981
Cac8I	GCNNGC	6 cut(s)	110, 173, 241, 456, 1072, 1483
CaiI	CAGNNNCTG	2 cut(s)	265, 770
CciI	TCATGA	1 cut(s)	312
CfoI	GCGC	1 cut(s)	684
Cfr10I	RCCGGY	1 cut(s)	1481
Cfr13I	GGNCC	4 cut(s)	127, 379, 692, 823
Cfr42I	CCGCGG	1 cut(s)	184
ClaI	ATCGAT	1 cut(s)	248
CseI	GACGC	1 cut(s)	1491
Csp6I	GTAC	2 cut(s)	210, 1336
CviAII	CATG	6 cut(s)	98, 313, 391, 661, 1096, 1471
CviQI	GTAC	2 cut(s)	210, 1336
DdeI	CTNAG	9 cut(s)	474, 540, 677, 906, 1044, 1136, 1265, 1538, 1581
DpnI	GATC	8 cut(s)	311, 428, 531, 921, 1094, 1182, 1320, 1564
DpnII	GATC	8 cut(s)	309, 426, 529, 919, 1092, 1180, 1318, 1562
DraIII	CACNNNGTG	1 cut(s)	517
DrdI	GACNNNNNNGTC	1 cut(s)	89
DseDI	GACNNNNNNGTC	1 cut(s)	89
EaeI	YGGCCR	4 cut(s)	166, 178, 830, 1159
EagI	CGGCCG	2 cut(s)	166, 178
Eam1104I	CTCTTC	1 cut(s)	422
EarI	CTCTTC	1 cut(s)	422
Ecl136II	GAGCTC	3 cut(s)	175, 397, 415
EclXI	CGGCCG	2 cut(s)	166, 178
Eco105I	TACGTA	1 cut(s)	360
Eco130I	CCWWGG	2 cut(s)	97, 1365
Eco24I	GRGCYC	3 cut(s)	177, 399, 417
Eco47I	GGWCC	4 cut(s)	127, 379, 692, 823
Eco52I	CGGCCG	2 cut(s)	166, 178
Eco53kI	GAGCTC	3 cut(s)	175, 397, 415
Eco57I	CTGAAG	2 cut(s)	717, 1395
Eco88I	CYCGRG	1 cut(s)	668
Eco91I	GGTNACC	1 cut(s)	220
EcoICRI	GAGCTC	3 cut(s)	175, 397, 415
EcoO109I	RGGNCCY	2 cut(s)	127, 823
EcoO65I	GGTNACC	1 cut(s)	220
EcoRI	GAATTC	1 cut(s)	188
EcoRII	CCWGG	3 cut(s)	434, 619, 827
EcoT14I	CCWWGG	2 cut(s)	97, 1365
EcoT22I	ATGCAT	1 cut(s)	525
EcoT38I	GRGCYC	3 cut(s)	177, 399, 417
ErhI	CCWWGG	2 cut(s)	97, 1365
Esp3I	CGTCTC	1 cut(s)	436
FaeI	CATG	6 cut(s)	101, 316, 394, 664, 1099, 1474
FaqI	GGGAC	1 cut(s)	836
FatI	CATG	6 cut(s)	97, 312, 390, 660, 1095, 1470
FbaI	TGATCA	1 cut(s)	1318
FokI	GGATG	6 cut(s)	302, 380, 1073, 1285, 1451, 1571
FriOI	GRGCYC	3 cut(s)	177, 399, 417
FspBI	CTAG	2 cut(s)	917, 1104
GlaI	GCGC	1 cut(s)	683
GsuI	CTGGAG	2 cut(s)	418, 738
HaeIII	GGCC	5 cut(s)	168, 180, 832, 1161, 1481
HapII	CCGG	1 cut(s)	1482
HgaI	GACGC	1 cut(s)	1491
HhaI	GCGC	1 cut(s)	684
Hin1II	CATG	6 cut(s)	101, 316, 394, 664, 1099, 1474
Hin6I	GCGC	1 cut(s)	682
HinP1I	GCGC	1 cut(s)	682
HindIII	AAGCTT	1 cut(s)	1013
HinfI	GANTC	2 cut(s)	195, 1168
HpaII	CCGG	1 cut(s)	1482
HphI	GGTGA	8 cut(s)	85, 190, 214, 422, 635, 980, 1217, 1253
Hpy166II	GTNNAC	3 cut(s)	280, 1028, 1261
Hpy188I	TCNGA	4 cut(s)	194, 844, 1167, 1539
Hpy8I	GTNNAC	3 cut(s)	280, 1028, 1261
HpyAV	CCTTC	6 cut(s)	118, 559, 577, 1263, 1304, 1471
HpyCH4III	ACNGT	4 cut(s)	515, 801, 976, 1535
HpyCH4IV	ACGT	4 cut(s)	155, 359, 1389, 1422
HpyCH4V	TGCA	9 cut(s)	243, 496, 523, 557, 785, 851, 934, 941, 1028
HpyF10VI	GCNNNNNNNGC	6 cut(s)	177, 265, 464, 584, 947, 1113
HpyF3I	CTNAG	9 cut(s)	474, 540, 677, 906, 1044, 1136, 1265, 1538, 1581
HpySE526I	ACGT	4 cut(s)	155, 359, 1389, 1422
Hsp92II	CATG	6 cut(s)	101, 316, 394, 664, 1099, 1474
HspAI	GCGC	1 cut(s)	682
KflI	GGGWCCC	1 cut(s)	823
KroI	GCCGGC	1 cut(s)	1481
KroNI	GCCGGC	1 cut(s)	1483
Ksp22I	TGATCA	1 cut(s)	1318
KspI	CCGCGG	1 cut(s)	184
Kzo9I	GATC	8 cut(s)	309, 426, 529, 919, 1092, 1180, 1318, 1562
LguI	GCTCTTC	1 cut(s)	422
LmnI	GCTCC	4 cut(s)	394, 670, 955, 1375
Lsp1109I	GCAGC	3 cut(s)	117, 252, 448
LweI	GCATC	3 cut(s)	510, 532, 861
MaeI	CTAG	2 cut(s)	917, 1104
MaeII	ACGT	4 cut(s)	155, 359, 1389, 1422
MaeIII	GTNAC	6 cut(s)	16, 91, 220, 469, 1053, 1451
MalI	GATC	8 cut(s)	311, 428, 531, 921, 1094, 1182, 1320, 1564
MbiI	CCGCTC	1 cut(s)	1525
MboI	GATC	8 cut(s)	309, 426, 529, 919, 1092, 1180, 1318, 1562
MboII	GAAGA	7 cut(s)	67, 409, 601, 877, 880, 1175, 1406
MfeI	CAATTG	1 cut(s)	588
MflI	RGATCY	1 cut(s)	529
MhlI	GDGCHC	5 cut(s)	177, 399, 417, 1030, 1293
MlsI	TGGCCA	2 cut(s)	832, 1161
MluCI	AATT	8 cut(s)	7, 188, 588, 639, 685, 792, 995, 1140
MluNI	TGGCCA	2 cut(s)	832, 1161
MmeI	TCCRAC	2 cut(s)	625, 1548
Mox20I	TGGCCA	2 cut(s)	832, 1161
Mph1103I	ATGCAT	1 cut(s)	525
MroNI	GCCGGC	1 cut(s)	1481
MscI	TGGCCA	2 cut(s)	832, 1161
MseI	TTAA	8 cut(s)	44, 615, 816, 876, 1017, 1089, 1122, 1588
Msp20I	TGGCCA	2 cut(s)	832, 1161
MspA1I	CMGCKG	4 cut(s)	183, 461, 467, 575
MspCI	CTTAAG	1 cut(s)	1016
MspI	CCGG	1 cut(s)	1482
MspR9I	CCNGG	3 cut(s)	436, 621, 829
MunI	CAATTG	1 cut(s)	588
Mva1269I	GAATGC	1 cut(s)	1304
MvaI	CCWGG	3 cut(s)	436, 621, 829
MvnI	CGCG	2 cut(s)	171, 183
MwoI	GCNNNNNNNGC	6 cut(s)	177, 265, 464, 584, 947, 1113
NaeI	GCCGGC	1 cut(s)	1483
NcoI	CCATGG	1 cut(s)	97
NdeII	GATC	8 cut(s)	309, 426, 529, 919, 1092, 1180, 1318, 1562
NgoMIV	GCCGGC	1 cut(s)	1481
NlaIII	CATG	6 cut(s)	101, 316, 394, 664, 1099, 1474
NlaIV	GGNNCC	6 cut(s)	129, 139, 666, 824, 825, 1371
NmeAIII	GCCGAG	2 cut(s)	144, 156
NmuCI	GTSAC	5 cut(s)	16, 91, 220, 469, 1053
NsiI	ATGCAT	1 cut(s)	525
NspV	TTCGAA	1 cut(s)	254
PagI	TCATGA	1 cut(s)	312
PciSI	GCTCTTC	1 cut(s)	422
PcsI	WCGNNNNNNNCGW	1 cut(s)	426
PctI	GAATGC	1 cut(s)	1304
PdiI	GCCGGC	1 cut(s)	1483
PfeI	GAWTC	2 cut(s)	195, 1168
PflFI	GACNNNGTC	1 cut(s)	218
Ppu21I	YACGTR	1 cut(s)	360
PpuMI	RGGWCCY	2 cut(s)	127, 823
PshAI	GACNNNNGTC	1 cut(s)	482
Psp124BI	GAGCTC	3 cut(s)	177, 399, 417
Psp1406I	AACGTT	1 cut(s)	1389
Psp5II	RGGWCCY	2 cut(s)	127, 823
Psp6I	CCWGG	3 cut(s)	434, 619, 827
PspEI	GGTNACC	1 cut(s)	220
PspGI	CCWGG	3 cut(s)	434, 619, 827
PspN4I	GGNNCC	6 cut(s)	129, 139, 666, 824, 825, 1371
PspPI	GGNCC	4 cut(s)	127, 379, 692, 823
PspPPI	RGGWCCY	2 cut(s)	127, 823
PstNI	CAGNNNCTG	2 cut(s)	265, 770
PsuI	RGATCY	1 cut(s)	529
PsyI	GACNNNGTC	1 cut(s)	218
RsaI	GTAC	2 cut(s)	211, 1337
RsaNI	GTAC	2 cut(s)	210, 1336
SacI	GAGCTC	3 cut(s)	177, 399, 417
SacII	CCGCGG	1 cut(s)	184
SapI	GCTCTTC	1 cut(s)	422
SaqAI	TTAA	8 cut(s)	44, 615, 816, 876, 1017, 1089, 1122, 1588
Sau3AI	GATC	8 cut(s)	309, 426, 529, 919, 1092, 1180, 1318, 1562
Sau96I	GGNCC	4 cut(s)	127, 379, 692, 823
ScrFI	CCNGG	3 cut(s)	436, 621, 829
SduI	GDGCHC	5 cut(s)	177, 399, 417, 1030, 1293
SfaNI	GCATC	3 cut(s)	510, 532, 861
Sfr303I	CCGCGG	1 cut(s)	184
SfuI	TTCGAA	1 cut(s)	254
SgrBI	CCGCGG	1 cut(s)	184
SinI	GGWCC	4 cut(s)	127, 379, 692, 823
SmlI	CTYRAG	3 cut(s)	989, 1016, 1031
SmoI	CTYRAG	3 cut(s)	989, 1016, 1031
SnaBI	TACGTA	1 cut(s)	360
Sse9I	AATT	8 cut(s)	7, 188, 588, 639, 685, 792, 995, 1140
SspI	AATATT	1 cut(s)	340
SspMI	CTAG	2 cut(s)	917, 1104
SstI	GAGCTC	3 cut(s)	177, 399, 417
StyD4I	CCNGG	3 cut(s)	434, 619, 827
StyI	CCWWGG	2 cut(s)	97, 1365
TaaI	ACNGT	4 cut(s)	515, 801, 976, 1535
TaiI	ACGT	4 cut(s)	158, 362, 1392, 1425
TaqI	TCGA	7 cut(s)	147, 248, 254, 285, 420, 486, 1308
TaqII	GACCGA	1 cut(s)	595
TasI	AATT	8 cut(s)	7, 188, 588, 639, 685, 792, 995, 1140
TatI	WGTACW	1 cut(s)	209
TauI	GCSGC	7 cut(s)	105, 171, 183, 461, 467, 578, 1469
TfiI	GAWTC	2 cut(s)	195, 1168
Tru1I	TTAA	8 cut(s)	44, 615, 816, 876, 1017, 1089, 1122, 1588
Tru9I	TTAA	8 cut(s)	44, 615, 816, 876, 1017, 1089, 1122, 1588
TscAI	CASTG	1 cut(s)	981
TseFI	GTSAC	5 cut(s)	16, 91, 220, 469, 1053
TseI	GCWGC	3 cut(s)	105, 265, 461
Tsp45I	GTSAC	5 cut(s)	16, 91, 220, 469, 1053
TspDTI	ATGAA	6 cut(s)	174, 329, 1022, 1077, 1175, 1490
TspGWI	ACGGA	1 cut(s)	106
TspRI	CASTG	1 cut(s)	981
Tth111I	GACNNNGTC	1 cut(s)	218
Vha464I	CTTAAG	1 cut(s)	1016
VneI	GTGCAC	1 cut(s)	1026
VpaK11BI	GGWCC	4 cut(s)	127, 379, 692, 823
XapI	RAATTY	1 cut(s)	188
XbaI	TCTAGA	1 cut(s)	916
XspI	CTAG	2 cut(s)	917, 1104
Zsp2I	ATGCAT	1 cut(s)	525

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.